Rh4DG172500

gene silencing by RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001623
Physical Location & Seq
Reverse (-)
34378636 .. 34381364
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG172500.1

Sequence Viewer

Length: 2676 bp
ATGTCGACGAAGGCGTTAATTGTAGCAGGAGAAAAACCTCTTGAACAAATTAACAACGCATCTACTCGTATACGTGTTCCCATGAAGAGGTCTGGCATTGGATCTAGAGGTAAAAGGATTCCACTTCTTACTAACCATTTCAAGGTGGGAGTGAACAAATATACAGATGACTTTTACCACTATAGTATTGCAATGCAATATGATGACGGAACTCCAGTTGAAGGAAAAGGCATTGGATGCAAAGTTCTTGATAAAATTAAAGAGATATACGGCATAGAGCTGGCAAACAAAGAATTTGCGTATGATGGAGAGAAGAGCTTGTTTACTGTTGGTCAACTCCCTCATAAAAGACTTGATTTCATGATCGTATTGGATGACATGTCATTGAGCAGGTCTGGGGGATCCAGACGCGGTTACTTTTTAGAAGAGAGGGAGGGTGATAGGAAGAGAGTAAAGCAGTCATTTGGTTCCAAAACAATAAAGGTGCAGTTCAACTTTGCTGCTATAATCCCAATGCAAGCAATTGCGAATGCCTTACGAGGTCAGGACTCTGACCATTTTCAGGAAGCAGTTAGAGTTCTTGACATCATTCTTAGGCAGAATGCAGCAAAGCAGGGTTGTCTCCTTGTTCGGCAGTCATTCTTCCATAACAAGCCGAGGAGCTTTATAGAGCTGGGAGGAGGGGTGTTAGGCTGTAGGGGTTTACATTCAAGCTTCCGAGCCACCCAAGGTGGCCTATGTCTGAATATGGATGTATCCACTACAATGATTATAAAACCTGGTCCTGTTGTGAATTTCCTCATTGAGAACCAAAATGTCAAAACTCCTTACCAGATTGATTGGACTAAGGCTAAGAGGATGCTTAAAAATCTGAAGATTGTGACCTATGCCTCCAAGGAGTACAAAATCACTGGATTGAGTGATAAACCCTGCAGACAGCAGACCTTCTTTTTGAAATCAAAAAAAGGACAGAATGGAGATGGAGTAGAGATCACAGTGTGTGAATACTTTGCTCAGTATAAGCAGATGCAGTTACGCGATTCAGCAGATTTTCCATGCATTAATGTTGGAAAGCCAAAGAAACCATCCTACTTTCCTCTTGAGCTTTGTGACTTGGTTTCTTTGCAACGCTATACCAAGTCATTGTCCAATATCCAAAGGGCTTCACTAGTGGAGAAGTCTAGGCAGAAGCCCCAGGAGCGAATGTCAACTTTGCGTGATGCACTGAGGAGTAGCAAGTATGAGGCTGACGTGATGCTTCGTTCTGCTGGAATATCGATTGGTACAGAACTCATGCAGGTTGAAGGTCGTGTGTTGTCAGCCCCAAATTTGAAAGTGGGAAATGGAGATGTCATTTTTCCTAGGAATGGGAGATGGAATTTCAACAATAAAAAACTAGTAGATCCTGTAAAACTAGAACGCTGGGCTGTTGTGAATTTCTCTGCTCGCTGTGACATTCGGTACCTGGTTGACACTATGATAAAGTGTGGAGATATGAAAGGAATTTCCATCAAGCCTCCATTTGGTGTGTTTGAAGAGAGTATTCAGAACAGACGTCAGTCAGCTCCGGTTAGAGTAGAAAAGATGTTTGAATACATTAAGCCCAAACTTCCAGGACCACCACAGTTTTTGTTGTGCATTCTCCCAGAGAGGAAGAATTCTGAGATATATGGTCCATGGAAAAGGAAAAACCTTTCAGACATTGGGATTGCAACGCAGTGCATTGCACCTACGAAGGTGAACGATCAATACATCACTAACGTGCTCCTGAAAATTAATGCAAAGCTAGGTGGAATGAACTCCTTGCTAACTATGGAGCATTCTCCTTCTCTACCCTTGGTTTCCAAGTCCCCTACTCTGATATTAGGGATGGATGTGTCACATGGCTCACCTGGGCAGTCAAATATGCCTTCTATTGCAGCAGTGGTCAGTTCTAGGCACTGGCCTTGGATTTCTCGTTACCGAGCTGCAGTTCGTACTCAGTCACCAAAAGTAGAAATGATTGCCAATCTGTTCAAGCCTGTGTCTGATAAAGAGGACCAAGGCATAGTCAAGGAACTTTTGCTAGACTTTTATAATACTTCAAACTACAGGAAGCCGGAGCAGATTATTATCTTCAGGGATGGAGTGAGTGAATCACAGTTCAACCAAGTCTTGAATCTGGAATTGGATCAGATTATTAAGGCCTGCAAGTTCATTGACGAGAGCTGGTTGCCAAAGTTCATGGTTATTATTGCACAAAAGCGACATCACACGAAATTCTTTCAGGCTGGTGGTTCTTCTGACAATGTTCCACCTGGGACTATCGTTGACAACAAAGTCTGCGATCCAAGAAACAATGATTTCTACTTGTGTTCTCATGCTGGGATGATTGGAACAAGTCGGCCTACTCATTATCATGTTCTATATGATGAACTTGGCTTTTCAGCAGATGACTTGCAAGATCTAGTTCATTCTTTGTCGTATGTTTATCAAAGGAGCACGACGGCCGTATCTCTAGTTGCTCCAATATGCTATGCCCATCTAGCAGCAGCACAGATGAGTCAGTTCATTAGGTTTGATGAAATGTCAGAGACTGCCTCAAGCCAAGGTAGTGTTACTGTTGCTGGAGATGTGCCAGTGCAGGAATTACCCAGGCTACATAAAAATGTCGTCAACTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

891

Amino Acids

100.28

Weight (kDa)

9.41

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 38 - 198 2.5e-23 N-terminal domain of argonaute
ArgoL1 PF08699 210 - 259 1.9e-16 Argonaute linker 1 domain
PAZ PF02170 265 - 390 7.4e-25 PAZ domain
ArgoL2 PF16488 400 - 445 1.6e-09 Argonaute linker 2 domain
Piwi PF02171 544 - 851 2.4e-105 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 773, 2076
AasI GACNNNNNNGTC 1 cut(s) 2318
AatII GACGTC 1 cut(s) 1558
Acc36I ACCTGC 2 cut(s) 381, 1288
Acc65I GGTACC 1 cut(s) 1461
AccB1I GGYRCC 1 cut(s) 1461
AccI GTMKAC 2 cut(s) 5, 70
AccII CGCG 2 cut(s) 411, 1038
AciI CCGC 1 cut(s) 411
AclWI GGATC 6 cut(s) 109, 396, 409, 1397, 2178, 2321
AcoI YGGCCR 1 cut(s) 2487
AcsI RAATTY 8 cut(s) 293, 793, 1327, 1378, 1435, 1503, 1657, 2258
AcuI CTGAAG 2 cut(s) 893, 2101
AcyI GRCGYC 1 cut(s) 1555
AdeI CACNNNGTG 1 cut(s) 999
AfaI GTAC 4 cut(s) 902, 1285, 1463, 1978
AfiI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1367, 1523
AflIII ACRYGT 2 cut(s) 73, 378
AhlI ACTAGT 2 cut(s) 1168, 1396
AjiI CACGTC 1 cut(s) 1252
AjnI CCWGG 7 cut(s) 778, 1194, 1464, 1612, 1891, 2296, 2633
AjuI GAANNNNNNNTTGG 2 cut(s) 2150, 2182
AleI CACNNNNGTG 1 cut(s) 1760
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
Alw21I GWGCWC 2 cut(s) 1767, 2483
Alw26I GTCTC 2 cut(s) 626, 2567
AlwI GGATC 6 cut(s) 109, 396, 409, 1397, 2178, 2321
AlwNI CAGNNNCTG 1 cut(s) 2576
AoxI GGCC 5 cut(s) 733, 1943, 2184, 2384, 2487
ApeKI GCWGC 6 cut(s) 500, 605, 1919, 1967, 2528, 2531
ApoI RAATTY 8 cut(s) 293, 793, 1327, 1378, 1435, 1503, 1657, 2258
ArsI GACNNNNNNTTYG 2 cut(s) 934, 966
AseI ATTAAT 2 cut(s) 1062, 1776
Asp718I GGTACC 1 cut(s) 1461
AspA2I CCTAGG 1 cut(s) 1361
AspS9I GGNCC 4 cut(s) 782, 1616, 1673, 2038
AsuHPI GGTGA 4 cut(s) 449, 1750, 1881, 1977
AvaII GGWCC 4 cut(s) 782, 1616, 1673, 2038
AvrII CCTAGG 1 cut(s) 1361
BaeI ACNNNNGTAYC 2 cut(s) 2475, 2508
BamHI GGATCC 1 cut(s) 401
BanI GGYRCC 1 cut(s) 1461
Bbv12I GWGCWC 2 cut(s) 1767, 2483
BbvI GCAGC 6 cut(s) 487, 617, 1931, 1954, 2540, 2543
BccI CCATC 8 cut(s) 299, 974, 1093, 1368, 1517, 1864, 2117, 2529
BceAI ACGGC 3 cut(s) 286, 2474, 2502
BciT130I CCWGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
BciVI GTATCC 1 cut(s) 766
BcoDI GTCTC 2 cut(s) 626, 2567
BcuI ACTAGT 2 cut(s) 1168, 1396
BfmI CTRYAG 5 cut(s) 181, 694, 931, 1968, 2089
BfuAI ACCTGC 2 cut(s) 381, 1288
BfuI GTATCC 1 cut(s) 766
BglII AGATCT 1 cut(s) 2443
BisI GCNGC 6 cut(s) 501, 606, 1920, 1968, 2529, 2532
BlnI CCTAGG 1 cut(s) 1361
BlsI GCNGC 6 cut(s) 502, 607, 1921, 1969, 2530, 2533
Bme1390I CCNGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
Bme18I GGWCC 4 cut(s) 782, 1616, 1673, 2038
BmgBI CACGTC 1 cut(s) 1252
BmgT120I GGNCC 4 cut(s) 782, 1616, 1673, 2038
BmiI GGNNCC 3 cut(s) 403, 469, 1463
BmrFI CCNGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
BmsI GCATC 6 cut(s) 68, 227, 849, 1017, 1210, 1245
BoxI GACNNNNGTC 1 cut(s) 1558
BplI GAGNNNNNCTC 2 cut(s) 2564, 2596
BpmI CTGGAG 2 cut(s) 198, 2628
BpuEI CTTGAG 2 cut(s) 1121, 2566
Bsa29I ATCGAT 1 cut(s) 1277
BsaAI YACGTR 1 cut(s) 74
BsaBI GATNNNNATC 1 cut(s) 2111
BsaHI GRCGYC 1 cut(s) 1555
BsaWI WCCGGW 1 cut(s) 1567
BsaXI ACNNNNNCTCC 6 cut(s) 669, 699, 969, 999, 1190, 1220
Bsc4I CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1367, 1523
Bse1I ACTGG 4 cut(s) 215, 916, 1946, 2618
Bse3DI GCAATG 2 cut(s) 198, 1722
Bse8I GATNNNNATC 1 cut(s) 2111
BseBI CCWGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
BseCI ATCGAT 1 cut(s) 1277
BseGI GGATG 9 cut(s) 242, 379, 757, 864, 1085, 1875, 1879, 2128, 2373
BseJI GATNNNNATC 1 cut(s) 2111
BseLI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1367, 1523
BseMI GCAATG 2 cut(s) 198, 1722
BseMII CTCAG 4 cut(s) 1028, 1217, 1653, 1994
BseNI ACTGG 4 cut(s) 215, 916, 1946, 2618
BseRI GAGGAG 3 cut(s) 673, 693, 1243
BseX3I CGGCCG 1 cut(s) 2487
BseXI GCAGC 6 cut(s) 487, 617, 1931, 1954, 2540, 2543
BseYI CCCAGC 3 cut(s) 673, 1422, 2363
BsgI GTGCAG 2 cut(s) 506, 2642
Bsh1236I CGCG 2 cut(s) 411, 1038
Bsh1285I CGRYCG 1 cut(s) 2490
BshFI GGCC 5 cut(s) 735, 1945, 2186, 2386, 2489
BshNI GGYRCC 1 cut(s) 1461
BshVI ATCGAT 1 cut(s) 1277
BsiEI CGRYCG 1 cut(s) 2490
BsiHKAI GWGCWC 2 cut(s) 1767, 2483
BsiSI CCGG 2 cut(s) 1568, 2099
BslFI GGGAC 2 cut(s) 1834, 2314
BslI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1367, 1523
BsmAI GTCTC 2 cut(s) 626, 2567
BsmFI GGGAC 2 cut(s) 1834, 2314
BsmI GAATGC 4 cut(s) 535, 607, 1638, 1819
BsnI GGCC 5 cut(s) 735, 1945, 2186, 2386, 2489
Bsp1286I GDGCHC 2 cut(s) 1767, 2483
Bsp143I GATC 9 cut(s) 101, 363, 401, 990, 1402, 1744, 2170, 2326, 2443
Bsp19I CCATGG 1 cut(s) 1676
BspACI CCGC 1 cut(s) 411
BspANI GGCC 5 cut(s) 735, 1945, 2186, 2386, 2489
BspCNI CTCAG 4 cut(s) 1027, 1218, 1654, 1993
BspDI ATCGAT 1 cut(s) 1277
BspFNI CGCG 2 cut(s) 411, 1038
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 3 cut(s) 403, 469, 1463
BspMAI CTGCAG 2 cut(s) 935, 1972
BspMI ACCTGC 2 cut(s) 381, 1288
BspPI GGATC 6 cut(s) 109, 396, 409, 1397, 2178, 2321
BspQI GCTCTTC 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 1461
BsrDI GCAATG 2 cut(s) 198, 1722
BsrI ACTGG 4 cut(s) 215, 916, 1946, 2618
BssMI GATC 9 cut(s) 101, 363, 401, 990, 1402, 1744, 2170, 2326, 2443
BssNAI GTATAC 1 cut(s) 71
BssNI GRCGYC 1 cut(s) 1555
BssT1I CCWWGG 8 cut(s) 727, 894, 1361, 1676, 1836, 1946, 2041, 2587
Bst1107I GTATAC 1 cut(s) 71
Bst2UI CCWGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
Bst4CI ACNGT 5 cut(s) 328, 997, 1626, 2142, 2602
Bst6I CTCTTC 5 cut(s) 80, 308, 420, 440, 1530
BstACI GRCGYC 1 cut(s) 1555
BstAPI GCANNNNNTGC 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 282, 519, 1447, 2188
BstDEI CTNAG 7 cut(s) 593, 846, 852, 1014, 1226, 1662, 1980
BstDSI CCRYGG 1 cut(s) 1676
BstF5I GGATG 9 cut(s) 242, 379, 757, 864, 1085, 1875, 1879, 2128, 2373
BstFNI CGCG 2 cut(s) 411, 1038
BstKTI GATC 9 cut(s) 104, 366, 404, 993, 1405, 1747, 2173, 2329, 2446
BstMAI GTCTC 2 cut(s) 626, 2567
BstMBI GATC 9 cut(s) 101, 363, 401, 990, 1402, 1744, 2170, 2326, 2443
BstMCI CGRYCG 1 cut(s) 2490
BstMWI GCNNNNNNNGC 3 cut(s) 237, 1198, 2525
BstNI CCWGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
BstNSI RCATGY 1 cut(s) 382
BstPAI GACNNNNGTC 1 cut(s) 1558
BstSCI CCNGG 7 cut(s) 778, 1194, 1464, 1612, 1891, 2296, 2633
BstSFI CTRYAG 5 cut(s) 181, 694, 931, 1968, 2089
BstUI CGCG 2 cut(s) 411, 1038
BstV1I GCAGC 6 cut(s) 487, 617, 1931, 1954, 2540, 2543
BstX2I RGATCY 4 cut(s) 101, 401, 1402, 2443
BstYI RGATCY 4 cut(s) 101, 401, 1402, 2443
BstZ17I GTATAC 1 cut(s) 71
BstZI CGGCCG 1 cut(s) 2487
Bsu15I ATCGAT 1 cut(s) 1277
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 5 cut(s) 735, 1945, 2186, 2386, 2489
BsuTUI ATCGAT 1 cut(s) 1277
BtgI CCRYGG 1 cut(s) 1676
BtrI CACGTC 1 cut(s) 1252
BtsCI GGATG 9 cut(s) 242, 379, 757, 864, 1085, 1875, 1879, 2128, 2373
BtsI GCAGTG 2 cut(s) 1724, 1929
BtsIMutI CAGTG 7 cut(s) 909, 1002, 1223, 1724, 1929, 1939, 2625
BveI ACCTGC 2 cut(s) 381, 1288
Cac8I GCNNGC 4 cut(s) 282, 519, 1447, 2188
CaiI CAGNNNCTG 1 cut(s) 2576
CciI TCATGA 1 cut(s) 360
Cfr13I GGNCC 4 cut(s) 782, 1616, 1673, 2038
ClaI ATCGAT 1 cut(s) 1277
CseI GACGC 1 cut(s) 417
CsiI ACCWGGT 2 cut(s) 778, 1464
Csp6I GTAC 4 cut(s) 901, 1284, 1462, 1977
CspCI CAANNNNNGTGG 2 cut(s) 1611, 1646
CviQI GTAC 4 cut(s) 901, 1284, 1462, 1977
DdeI CTNAG 7 cut(s) 593, 846, 852, 1014, 1226, 1662, 1980
DpnI GATC 9 cut(s) 103, 365, 403, 992, 1404, 1746, 2172, 2328, 2445
DpnII GATC 9 cut(s) 101, 363, 401, 990, 1402, 1744, 2170, 2326, 2443
DraIII CACNNNGTG 1 cut(s) 999
DrdI GACNNNNNNGTC 1 cut(s) 2318
DseDI GACNNNNNNGTC 1 cut(s) 2318
EaeI YGGCCR 1 cut(s) 2487
EagI CGGCCG 1 cut(s) 2487
Eam1104I CTCTTC 5 cut(s) 80, 308, 420, 440, 1530
EarI CTCTTC 5 cut(s) 80, 308, 420, 440, 1530
EclXI CGGCCG 1 cut(s) 2487
Eco130I CCWWGG 8 cut(s) 727, 894, 1361, 1676, 1836, 1946, 2041, 2587
Eco147I AGGCCT 1 cut(s) 2186
Eco47I GGWCC 4 cut(s) 782, 1616, 1673, 2038
Eco52I CGGCCG 1 cut(s) 2487
Eco57I CTGAAG 2 cut(s) 893, 2101
EcoRI GAATTC 1 cut(s) 1657
EcoRII CCWGG 7 cut(s) 778, 1194, 1464, 1612, 1891, 2296, 2633
EcoT14I CCWWGG 8 cut(s) 727, 894, 1361, 1676, 1836, 1946, 2041, 2587
EcoT22I ATGCAT 1 cut(s) 1061
ErhI CCWWGG 8 cut(s) 727, 894, 1361, 1676, 1836, 1946, 2041, 2587
FaqI GGGAC 2 cut(s) 1834, 2314
FblI GTMKAC 2 cut(s) 5, 70
Fnu4HI GCNGC 6 cut(s) 501, 606, 1920, 1968, 2529, 2532
FokI GGATG 9 cut(s) 249, 386, 764, 871, 1072, 1882, 1886, 2135, 2380
Fsp4HI GCNGC 6 cut(s) 501, 606, 1920, 1968, 2529, 2532
GluI GCNGC 6 cut(s) 501, 606, 1920, 1968, 2529, 2532
GsaI CCCAGC 3 cut(s) 677, 1426, 2367
GsuI CTGGAG 2 cut(s) 198, 2628
HaeIII GGCC 5 cut(s) 735, 1945, 2186, 2386, 2489
HapII CCGG 2 cut(s) 1568, 2099
HgaI GACGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 1555
HincII GTYRAC 6 cut(s) 6, 335, 1209, 1471, 2311, 2656
HindII GTYRAC 6 cut(s) 6, 335, 1209, 1471, 2311, 2656
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 6 cut(s) 118, 548, 1040, 2135, 2158, 2542
HpaII CCGG 2 cut(s) 1568, 2099
HphI GGTGA 4 cut(s) 449, 1750, 1881, 1977
Hpy99I CGWCG 2 cut(s) 10, 2488
HpyAV CCTTC 7 cut(s) 4, 215, 955, 1298, 1729, 1836, 1920
HpyCH4III ACNGT 5 cut(s) 328, 997, 1626, 2142, 2602
HpyCH4IV ACGT 4 cut(s) 73, 1251, 1555, 1761
HpyF10VI GCNNNNNNNGC 3 cut(s) 237, 1198, 2525
HpyF3I CTNAG 7 cut(s) 593, 846, 852, 1014, 1226, 1662, 1980
HpySE526I ACGT 4 cut(s) 73, 1251, 1555, 1761
Hsp92I GRCGYC 1 cut(s) 1555
KpnI GGTACC 1 cut(s) 1465
Kzo9I GATC 9 cut(s) 101, 363, 401, 990, 1402, 1744, 2170, 2326, 2443
LguI GCTCTTC 1 cut(s) 308
LmnI GCTCC 8 cut(s) 660, 1198, 1570, 1770, 1816, 2101, 2478, 2509
Lsp1109I GCAGC 6 cut(s) 487, 617, 1931, 1954, 2540, 2543
LweI GCATC 6 cut(s) 68, 227, 849, 1017, 1210, 1245
MabI ACCWGGT 2 cut(s) 778, 1464
MaeII ACGT 4 cut(s) 73, 1251, 1555, 1761
MaeIII GTNAC 9 cut(s) 413, 880, 1032, 1109, 1451, 1878, 1958, 1983, 2596
MalI GATC 9 cut(s) 103, 365, 403, 992, 1404, 1746, 2172, 2328, 2445
MboI GATC 9 cut(s) 101, 363, 401, 990, 1402, 1744, 2170, 2326, 2443
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 4 cut(s) 101, 401, 1402, 2443
MhlI GDGCHC 2 cut(s) 1767, 2483
MlyI GAGTC 2 cut(s) 542, 2551
MmeI TCCRAC 1 cut(s) 1048
Mph1103I ATGCAT 1 cut(s) 1061
MseI TTAA 8 cut(s) 17, 51, 258, 864, 1062, 1599, 1776, 2181
MslI CAYNNNNRTG 4 cut(s) 764, 1760, 2397, 2646
MspI CCGG 2 cut(s) 1568, 2099
MspR9I CCNGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 4 cut(s) 535, 607, 1638, 1819
MvaI CCWGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
MvnI CGCG 2 cut(s) 411, 1038
MwoI GCNNNNNNNGC 3 cut(s) 237, 1198, 2525
NcoI CCATGG 1 cut(s) 1676
NdeII GATC 9 cut(s) 101, 363, 401, 990, 1402, 1744, 2170, 2326, 2443
NlaIV GGNNCC 3 cut(s) 403, 469, 1463
NmeAIII GCCGAG 1 cut(s) 681
NmuCI GTSAC 5 cut(s) 880, 1109, 1451, 1878, 1983
NsiI ATGCAT 1 cut(s) 1061
NspI RCATGY 1 cut(s) 382
OliI CACNNNNGTG 1 cut(s) 1760
PagI TCATGA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 2186
PciI ACATGT 1 cut(s) 378
PciSI GCTCTTC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 4 cut(s) 535, 607, 1638, 1819
PfeI GAWTC 4 cut(s) 118, 1040, 2135, 2158
PfoI TCCNGGA 1 cut(s) 1612
PkrI GCNGC 6 cut(s) 502, 607, 1921, 1969, 2530, 2533
PleI GAGTC 2 cut(s) 542, 2550
PpsI GAGTC 2 cut(s) 542, 2550
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 1 cut(s) 378
PshAI GACNNNNGTC 1 cut(s) 1558
PshBI ATTAAT 2 cut(s) 1062, 1776
PsiI TTATAA 2 cut(s) 773, 2076
Psp6I CCWGG 7 cut(s) 778, 1194, 1464, 1612, 1891, 2296, 2633
PspFI CCCAGC 3 cut(s) 673, 1422, 2363
PspGI CCWGG 7 cut(s) 778, 1194, 1464, 1612, 1891, 2296, 2633
PspN4I GGNNCC 3 cut(s) 403, 469, 1463
PspPI GGNCC 4 cut(s) 782, 1616, 1673, 2038
PsrI GAACNNNNNNTAC 2 cut(s) 1733, 1765
PstI CTGCAG 2 cut(s) 935, 1972
PstNI CAGNNNCTG 1 cut(s) 2576
PsuI RGATCY 4 cut(s) 101, 401, 1402, 2443
RsaI GTAC 4 cut(s) 902, 1285, 1463, 1978
RsaNI GTAC 4 cut(s) 901, 1284, 1462, 1977
RseI CAYNNNNRTG 4 cut(s) 764, 1760, 2397, 2646
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 308
SaqAI TTAA 8 cut(s) 17, 51, 258, 864, 1062, 1599, 1776, 2181
SatI GCNGC 6 cut(s) 501, 606, 1920, 1968, 2529, 2532
Sau3AI GATC 9 cut(s) 101, 363, 401, 990, 1402, 1744, 2170, 2326, 2443
Sau96I GGNCC 4 cut(s) 782, 1616, 1673, 2038
SchI GAGTC 2 cut(s) 542, 2551
ScrFI CCNGG 7 cut(s) 780, 1196, 1466, 1614, 1893, 2298, 2635
SduI GDGCHC 2 cut(s) 1767, 2483
SexAI ACCWGGT 2 cut(s) 778, 1464
SfaNI GCATC 6 cut(s) 68, 227, 849, 1017, 1210, 1245
SfcI CTRYAG 5 cut(s) 181, 694, 931, 1968, 2089
SinI GGWCC 4 cut(s) 782, 1616, 1673, 2038
SmiMI CAYNNNNRTG 4 cut(s) 764, 1760, 2397, 2646
SmlI CTYRAG 2 cut(s) 1100, 2581
SmoI CTYRAG 2 cut(s) 1100, 2581
SpeI ACTAGT 2 cut(s) 1168, 1396
SseBI AGGCCT 1 cut(s) 2186
SsiI CCGC 1 cut(s) 411
StuI AGGCCT 1 cut(s) 2186
StyD4I CCNGG 7 cut(s) 778, 1194, 1464, 1612, 1891, 2296, 2633
StyI CCWWGG 8 cut(s) 727, 894, 1361, 1676, 1836, 1946, 2041, 2587
TaaI ACNGT 5 cut(s) 328, 997, 1626, 2142, 2602
TaiI ACGT 4 cut(s) 76, 1254, 1558, 1764
TaqI TCGA 2 cut(s) 5, 1277
TatI WGTACW 1 cut(s) 900
TfiI GAWTC 4 cut(s) 118, 1040, 2135, 2158
Tru1I TTAA 8 cut(s) 17, 51, 258, 864, 1062, 1599, 1776, 2181
Tru9I TTAA 8 cut(s) 17, 51, 258, 864, 1062, 1599, 1776, 2181
TscAI CASTG 7 cut(s) 916, 1002, 1230, 1724, 1929, 1946, 2625
TseFI GTSAC 5 cut(s) 880, 1109, 1451, 1878, 1983
TseI GCWGC 6 cut(s) 500, 605, 1919, 1967, 2528, 2531
Tsp45I GTSAC 5 cut(s) 880, 1109, 1451, 1878, 1983
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 7 cut(s) 916, 1002, 1230, 1724, 1929, 1946, 2625
VpaK11BI GGWCC 4 cut(s) 782, 1616, 1673, 2038
VspI ATTAAT 2 cut(s) 1062, 1776
XapI RAATTY 8 cut(s) 293, 793, 1327, 1378, 1435, 1503, 1657, 2258
XbaI TCTAGA 1 cut(s) 104
XceI RCATGY 1 cut(s) 382
XmaJI CCTAGG 1 cut(s) 1361
XmiI GTMKAC 2 cut(s) 5, 70
ZraI GACGTC 1 cut(s) 1556
Zsp2I ATGCAT 1 cut(s) 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.