Rroxscaffold_5G00357050

gene silencing by RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001623
Physical Location & Seq
Reverse (-)
36537977 .. 36540655
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00357050.1

Sequence Viewer

Length: 2679 bp
ATGTCGACGAAGGCGTTAATTGTAGCAGGAGAAAAGCCTCTTGAACAAATTAACAACTCATCTACTCAAATACGTGTTCCCATGAAGAGGTCTGGCATTGGATCTAGAGGTAAAAGGATTCCACTTCTTACCAACCATTTCAAGGTGGGAGTGAACAATTATACAGATGACTTTTACCACTATAGTATTGCAATGCAATATGATGACGGAACTCCAGTTGATGGAAAAGGCATTGCATGCAAAGTTCTTGATAAAATTAAAGAGATATACGGCATGGAGCTGGCAAACAAAGAATTTGCTTATGATGGAGAGAAGAGCTTGTTTACTGTTGGTCAACTCCCTCATAAAAGACTTGATTTCATGATCGTATTGGATGACATGTCATTGAGAAGGTCTGGGGGATCCAGACGCGGTTACTTTCTAGAAGAGAGGGAGGGTGATATGAAGAGAGTAAAGCAGTCATTTGGTTCCAAAACAATAAAGGTGCAGTTCAACTTTGCTGCTATAATCCCAATGCAAGCAGTTGCGAATGCCTTACGAGGTCAGGACTCTGACCATTTTCAGGAAGCAGTTAGAGTTCTTGACATCATTCTAAGGCAGAATGCAGCAAAGCAGGGTTGTCTCCTTGTTCGGCAGTCATTCTTCCATAACAAGCCGAGGAGCTTTATAGAGTTGGGAGGAGGGCTGTTAGGCTGTAGGGGTTTCCATTCAAGCTTCCGGGCCACCCAAGGCGGCCTAAGTCTGAATATGGATGTATCCACAACAATGATTCTAAAACCTGGTCCTGTTGTGAATTTCCTCATGGAGAACCAACATATCAAAACTCCTTACCAGATTGATTGGACTAAGGCTAAGAGGATGCTTAAAAATCTGAAGATTGTGACCTATGCCTCCAAGAAGGAGTACAAAATCACTGGATTGAGTGATAAACCCTGCAGACAGCAGACCTTCTTTTTGAAATCAAAAAAAGGACAGAATGGAGATGGAGTAGAGATCACAGTGTGTGAATACTTTGCTCAGTATAAGCAGATGCAGTTACGCGATTCAGCAGATTTTCCATGCATTAATGTTGGAAAGCCAAAGAAACCATCCTACTTTCCACTTGAGCTTTGTGACTTGGTTTCTTTGCAACGCTATACCAAGTCCTTGTCCAATATCCAAAGGGCTTCACTAGTGGAGAAGTCTAGGCAGAAGCCCCAGGAACGAATGTCAACTTTGCGTGATGCACTGAGGAGTAGCAAGTATGAGGCTGACGTGATGCTTCGTTCTGCTGGAATATCGATTGGTACAGAACTCATGCAGGTTGAAGGTCGTGTGTTGTCAGCCCCAAATTTGAAAGTGGGAAATGGAGATGTCATTTTTCCTAGGAATGGGAGATGGAATTTCAACAATAAAAAACTAGTAGATCCTGTAAAACTAGAACGCTGGGCTGTTGTGAATTTCTCTGCTCGCTGTGACATTCGGTACCTGGTCGACACTATGATAAAGTGTGGAGATATGAAAGGAATTTCCATCAAGCCTCCATTTGGTGTGTTTGAAGAGAGTATTCAGAACAGACGTCAGTCAGCTCCGGTTAGAGTAGAAAAGATGTTTGAATGCATTAAGCCCAAACTTCCAGGACCACCACAGTTTTTGTTATGCATTCTCCCAGAGAGGAAGAATTCTGAGATATATGGTCCATGGAAAAGGAAAAACCTTTCAGACATTGGGATTGCAACGCAGTGCATTGCGCCTACGAAGGTGAACGATCAATACATCACTAACGTGCTCCTAAAAATTAATGCAAAGTTAGGTGGAATGAACTCCTTGCTAACTGTGGAGCATGCTCCTTCTCTACCCTTGGTTTCCAAGTCCCCTACTCTGATATTAGGGATGGATGTGTCACATGGCTCACCTGGGCAGTCAAATATGCCTTCTATTGCAGCAGTGGTCAGTTCTAGGCACTGGCCTTGGATTTCTCGTTACCGAGCTGCAGTTCGTACTCAGTCACCAAAAGTAGAAATCATTGCCAATCTGTTCAAGCCTGTGTCTGATAAAGAGGACCAAGGCATAGTCAAGGAACTTTTGCTAGACTTTTATAATACTTCAAACTACAGGAAGCCGGAGCAGATTATTATTTTCAGGGATGGAGTGAGTGAATCACAGTTCAACCAAGTCTTGAATCTGGAATTGGATCAGATTATTAAGGCCTGCAAGTTCATTGACGAGAGCTGGTCGCCAAAGTTCATGGTTATTATTGCACAAAAGAGACATCACACAAAATTCTTTCAGGCTGGTGGTTCTTCTGATAATGTTCCACCTGGGACTATCGTTGACAACAAAGTCTGTGATCCAAGAAACAATGATTTCTACTTGTGTTCTCACGCTGGGATAATTGGAACAAGTCGGCCAACTCATTATCATGTTCTATATGATGAACTTGGCTTTTCAGCAGATGACTTGCAAGAACTAGTTCATTCTTTGTCGTATGTTTATCAAAGGAGCACGACAGCCGTATCTCTAGTTGCTCCAATATGCTATGCCCATCTAGCAGCTGCACAGATGAGTCAGTTCATTAGGTTTGATGAAATGTCAGAGACTACCTCAAGCCAAGGTAGTGTTACTGTTGCTGAAGATGTGCCAGTGCAGGAATTACCCAAGCTACATAAAAATGTCGTCAACTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

892

Amino Acids

100.43

Weight (kDa)

9.36

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 38 - 198 1.4e-22 N-terminal domain of argonaute
ArgoL1 PF08699 210 - 258 8.7e-18 Argonaute linker 1 domain
PAZ PF02170 265 - 391 2.3e-25 PAZ domain
ArgoL2 PF16488 401 - 446 1.6e-09 Argonaute linker 2 domain
Piwi PF02171 545 - 852 3.2e-105 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2079
AasI GACNNNNNNGTC 1 cut(s) 2321
AatII GACGTC 1 cut(s) 1561
Acc36I ACCTGC 1 cut(s) 1291
Acc65I GGTACC 1 cut(s) 1464
AccB1I GGYRCC 1 cut(s) 1464
AccB7I CCANNNNNTGG 1 cut(s) 221
AccI GTMKAC 2 cut(s) 5, 1473
AccII CGCG 2 cut(s) 411, 1041
AciI CCGC 2 cut(s) 411, 732
AclWI GGATC 6 cut(s) 109, 396, 409, 1400, 2181, 2324
AcoI YGGCCR 1 cut(s) 2387
AcsI RAATTY 8 cut(s) 293, 793, 1330, 1381, 1438, 1506, 1660, 2261
AcuI CTGAAG 2 cut(s) 893, 2631
AcyI GRCGYC 1 cut(s) 1558
AdeI CACNNNGTG 1 cut(s) 1002
AfaI GTAC 4 cut(s) 905, 1288, 1466, 1981
AfiI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1370, 1526
AflIII ACRYGT 2 cut(s) 73, 378
AhlI ACTAGT 3 cut(s) 1171, 1399, 2449
AjiI CACGTC 1 cut(s) 1255
AjnI CCWGG 6 cut(s) 778, 1197, 1467, 1615, 1894, 2299
AjuI GAANNNNNNNTTGG 2 cut(s) 2153, 2185
AleI CACNNNNGTG 1 cut(s) 1763
Alw21I GWGCWC 2 cut(s) 1770, 2486
Alw26I GTCTC 3 cut(s) 626, 2242, 2570
AlwI GGATC 6 cut(s) 109, 396, 409, 1400, 2181, 2324
AoxI GGCC 5 cut(s) 720, 733, 1946, 2187, 2387
ApeKI GCWGC 6 cut(s) 500, 605, 1922, 1970, 2531, 2534
ApoI RAATTY 8 cut(s) 293, 793, 1330, 1381, 1438, 1506, 1660, 2261
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AseI ATTAAT 2 cut(s) 1065, 1779
Asp700I GAANNNNTTC 1 cut(s) 2451
Asp718I GGTACC 1 cut(s) 1464
AspA2I CCTAGG 1 cut(s) 1364
AspLEI GCGC 1 cut(s) 1732
AspS9I GGNCC 5 cut(s) 720, 782, 1619, 1676, 2041
AsuC2I CCSGG 1 cut(s) 719
AsuHPI GGTGA 4 cut(s) 449, 1753, 1884, 1980
AvaII GGWCC 4 cut(s) 782, 1619, 1676, 2041
AvrII CCTAGG 1 cut(s) 1364
BaeI ACNNNNGTAYC 2 cut(s) 2478, 2511
BamHI GGATCC 1 cut(s) 401
BanI GGYRCC 1 cut(s) 1464
Bbv12I GWGCWC 2 cut(s) 1770, 2486
BbvI GCAGC 6 cut(s) 487, 617, 1934, 1957, 2521, 2543
BccI CCATC 9 cut(s) 215, 299, 977, 1096, 1371, 1520, 1867, 2120, 2532
BceAI ACGGC 2 cut(s) 286, 2477
BciT130I CCWGG 6 cut(s) 780, 1199, 1469, 1617, 1896, 2301
BciVI GTATCC 1 cut(s) 766
BcnI CCSGG 1 cut(s) 719
BcoDI GTCTC 3 cut(s) 626, 2242, 2570
BcuI ACTAGT 3 cut(s) 1171, 1399, 2449
BfmI CTRYAG 5 cut(s) 181, 694, 934, 1971, 2092
BfuAI ACCTGC 1 cut(s) 1291
BfuI GTATCC 1 cut(s) 766
BisI GCNGC 7 cut(s) 501, 606, 733, 1923, 1971, 2532, 2535
BlnI CCTAGG 1 cut(s) 1364
BlsI GCNGC 7 cut(s) 502, 607, 734, 1924, 1972, 2533, 2536
Bme1390I CCNGG 7 cut(s) 719, 780, 1199, 1469, 1617, 1896, 2301
Bme18I GGWCC 4 cut(s) 782, 1619, 1676, 2041
BmgBI CACGTC 1 cut(s) 1255
BmgT120I GGNCC 5 cut(s) 720, 782, 1619, 1676, 2041
BmiI GGNNCC 3 cut(s) 403, 469, 1466
BmrFI CCNGG 7 cut(s) 719, 780, 1199, 1469, 1617, 1896, 2301
BmsI GCATC 4 cut(s) 849, 1020, 1213, 1248
BoxI GACNNNNGTC 1 cut(s) 1561
BplI GAGNNNNNCTC 2 cut(s) 2567, 2599
BpmI CTGGAG 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 1124, 2569
BpuMI CCSGG 1 cut(s) 719
Bsa29I ATCGAT 1 cut(s) 1280
BsaAI YACGTR 1 cut(s) 74
BsaHI GRCGYC 1 cut(s) 1558
BsaWI WCCGGW 1 cut(s) 1570
BsaXI ACNNNNNCTCC 2 cut(s) 972, 1002
Bsc4I CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1370, 1526
Bse1I ACTGG 4 cut(s) 215, 919, 1949, 2621
Bse3DI GCAATG 4 cut(s) 198, 231, 1725, 2004
BseBI CCWGG 6 cut(s) 780, 1199, 1469, 1617, 1896, 2301
BseCI ATCGAT 1 cut(s) 1280
BseGI GGATG 7 cut(s) 379, 757, 864, 1088, 1878, 1882, 2131
BseLI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1370, 1526
BseMI GCAATG 4 cut(s) 198, 231, 1725, 2004
BseMII CTCAG 4 cut(s) 1031, 1220, 1656, 1997
BseNI ACTGG 4 cut(s) 215, 919, 1949, 2621
BseRI GAGGAG 3 cut(s) 673, 693, 1246
BseXI GCAGC 6 cut(s) 487, 617, 1934, 1957, 2521, 2543
BseYI CCCAGC 2 cut(s) 1425, 2366
BsgI GTGCAG 3 cut(s) 506, 2520, 2645
Bsh1236I CGCG 2 cut(s) 411, 1041
BshFI GGCC 5 cut(s) 722, 735, 1948, 2189, 2389
BshNI GGYRCC 1 cut(s) 1464
BshVI ATCGAT 1 cut(s) 1280
BsiHKAI GWGCWC 2 cut(s) 1770, 2486
BsiSI CCGG 3 cut(s) 718, 1571, 2102
BslFI GGGAC 2 cut(s) 1837, 2317
BslI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1370, 1526
BsmAI GTCTC 3 cut(s) 626, 2242, 2570
BsmFI GGGAC 2 cut(s) 1837, 2317
BsmI GAATGC 4 cut(s) 535, 607, 1601, 1641
BsnI GGCC 5 cut(s) 722, 735, 1948, 2189, 2389
Bsp1286I GDGCHC 2 cut(s) 1770, 2486
Bsp143I GATC 8 cut(s) 101, 363, 401, 993, 1405, 1747, 2173, 2329
Bsp19I CCATGG 1 cut(s) 1679
BspACI CCGC 2 cut(s) 411, 732
BspANI GGCC 5 cut(s) 722, 735, 1948, 2189, 2389
BspCNI CTCAG 4 cut(s) 1030, 1221, 1657, 1996
BspDI ATCGAT 1 cut(s) 1280
BspFNI CGCG 2 cut(s) 411, 1041
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 3 cut(s) 403, 469, 1466
BspMAI CTGCAG 2 cut(s) 938, 1975
BspMI ACCTGC 1 cut(s) 1291
BspPI GGATC 6 cut(s) 109, 396, 409, 1400, 2181, 2324
BspQI GCTCTTC 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 1464
BsrDI GCAATG 4 cut(s) 198, 231, 1725, 2004
BsrI ACTGG 4 cut(s) 215, 919, 1949, 2621
BssMI GATC 8 cut(s) 101, 363, 401, 993, 1405, 1747, 2173, 2329
BssNI GRCGYC 1 cut(s) 1558
BssT1I CCWWGG 7 cut(s) 727, 1364, 1679, 1839, 1949, 2044, 2590
Bst2UI CCWGG 6 cut(s) 780, 1199, 1469, 1617, 1896, 2301
Bst4CI ACNGT 6 cut(s) 328, 1000, 1629, 1816, 2145, 2605
Bst6I CTCTTC 5 cut(s) 80, 308, 420, 440, 1533
BstACI GRCGYC 1 cut(s) 1558
BstAPI GCANNNNNTGC 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 6 cut(s) 238, 282, 519, 1450, 1824, 2191
BstDEI CTNAG 8 cut(s) 593, 737, 846, 852, 1017, 1229, 1665, 1983
BstDSI CCRYGG 1 cut(s) 1679
BstF5I GGATG 7 cut(s) 379, 757, 864, 1088, 1878, 1882, 2131
BstFNI CGCG 2 cut(s) 411, 1041
BstHHI GCGC 1 cut(s) 1732
BstKTI GATC 8 cut(s) 104, 366, 404, 996, 1408, 1750, 2176, 2332
BstMAI GTCTC 3 cut(s) 626, 2242, 2570
BstMBI GATC 8 cut(s) 101, 363, 401, 993, 1405, 1747, 2173, 2329
BstMWI GCNNNNNNNGC 2 cut(s) 237, 2528
BstNI CCWGG 6 cut(s) 780, 1199, 1469, 1617, 1896, 2301
BstNSI RCATGY 3 cut(s) 240, 382, 1826
BstPAI GACNNNNGTC 1 cut(s) 1561
BstSCI CCNGG 7 cut(s) 717, 778, 1197, 1467, 1615, 1894, 2299
BstSFI CTRYAG 5 cut(s) 181, 694, 934, 1971, 2092
BstUI CGCG 2 cut(s) 411, 1041
BstV1I GCAGC 6 cut(s) 487, 617, 1934, 1957, 2521, 2543
BstX2I RGATCY 3 cut(s) 101, 401, 1405
BstYI RGATCY 3 cut(s) 101, 401, 1405
Bsu15I ATCGAT 1 cut(s) 1280
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 5 cut(s) 722, 735, 1948, 2189, 2389
BsuTUI ATCGAT 1 cut(s) 1280
BtgI CCRYGG 1 cut(s) 1679
BtrI CACGTC 1 cut(s) 1255
BtsCI GGATG 7 cut(s) 379, 757, 864, 1088, 1878, 1882, 2131
BtsI GCAGTG 2 cut(s) 1727, 1932
BtsIMutI CAGTG 7 cut(s) 912, 1005, 1226, 1727, 1932, 1942, 2628
BveI ACCTGC 1 cut(s) 1291
Cac8I GCNNGC 6 cut(s) 238, 282, 519, 1450, 1824, 2191
CciI TCATGA 1 cut(s) 360
CfoI GCGC 1 cut(s) 1732
Cfr13I GGNCC 5 cut(s) 720, 782, 1619, 1676, 2041
ClaI ATCGAT 1 cut(s) 1280
CseI GACGC 1 cut(s) 417
CsiI ACCWGGT 2 cut(s) 778, 1467
Csp6I GTAC 4 cut(s) 904, 1287, 1465, 1980
CspCI CAANNNNNGTGG 2 cut(s) 1614, 1649
CviQI GTAC 4 cut(s) 904, 1287, 1465, 1980
DdeI CTNAG 8 cut(s) 593, 737, 846, 852, 1017, 1229, 1665, 1983
DpnI GATC 8 cut(s) 103, 365, 403, 995, 1407, 1749, 2175, 2331
DpnII GATC 8 cut(s) 101, 363, 401, 993, 1405, 1747, 2173, 2329
DraIII CACNNNGTG 1 cut(s) 1002
DrdI GACNNNNNNGTC 1 cut(s) 2321
DseDI GACNNNNNNGTC 1 cut(s) 2321
EaeI YGGCCR 1 cut(s) 2387
Eam1104I CTCTTC 5 cut(s) 80, 308, 420, 440, 1533
EarI CTCTTC 5 cut(s) 80, 308, 420, 440, 1533
Eco130I CCWWGG 7 cut(s) 727, 1364, 1679, 1839, 1949, 2044, 2590
Eco147I AGGCCT 1 cut(s) 2189
Eco47I GGWCC 4 cut(s) 782, 1619, 1676, 2041
Eco57I CTGAAG 2 cut(s) 893, 2631
EcoRI GAATTC 1 cut(s) 1660
EcoRII CCWGG 6 cut(s) 778, 1197, 1467, 1615, 1894, 2299
EcoT14I CCWWGG 7 cut(s) 727, 1364, 1679, 1839, 1949, 2044, 2590
EcoT22I ATGCAT 3 cut(s) 1064, 1601, 1643
ErhI CCWWGG 7 cut(s) 727, 1364, 1679, 1839, 1949, 2044, 2590
FaqI GGGAC 2 cut(s) 1837, 2317
FblI GTMKAC 2 cut(s) 5, 1473
Fnu4HI GCNGC 7 cut(s) 501, 606, 733, 1923, 1971, 2532, 2535
FokI GGATG 7 cut(s) 386, 764, 871, 1075, 1885, 1889, 2138
Fsp4HI GCNGC 7 cut(s) 501, 606, 733, 1923, 1971, 2532, 2535
GlaI GCGC 1 cut(s) 1731
GluI GCNGC 7 cut(s) 501, 606, 733, 1923, 1971, 2532, 2535
GsaI CCCAGC 2 cut(s) 1429, 2370
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 5 cut(s) 722, 735, 1948, 2189, 2389
HapII CCGG 3 cut(s) 718, 1571, 2102
HgaI GACGC 1 cut(s) 417
HhaI GCGC 1 cut(s) 1732
Hin1I GRCGYC 1 cut(s) 1558
Hin6I GCGC 1 cut(s) 1730
HinP1I GCGC 1 cut(s) 1730
HincII GTYRAC 6 cut(s) 6, 335, 1212, 1474, 2314, 2659
HindII GTYRAC 6 cut(s) 6, 335, 1212, 1474, 2314, 2659
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 7 cut(s) 118, 548, 769, 1043, 2138, 2161, 2545
HpaII CCGG 3 cut(s) 718, 1571, 2102
HphI GGTGA 4 cut(s) 449, 1753, 1884, 1980
Hpy166II GTNNAC 9 cut(s) 6, 154, 324, 335, 1212, 1474, 1744, 2314, 2659
Hpy8I GTNNAC 9 cut(s) 6, 154, 324, 335, 1212, 1474, 1744, 2314, 2659
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 8 cut(s) 4, 384, 892, 958, 1301, 1732, 1839, 1923
HpyCH4III ACNGT 6 cut(s) 328, 1000, 1629, 1816, 2145, 2605
HpyCH4IV ACGT 4 cut(s) 73, 1254, 1558, 1764
HpyF10VI GCNNNNNNNGC 2 cut(s) 237, 2528
HpyF3I CTNAG 8 cut(s) 593, 737, 846, 852, 1017, 1229, 1665, 1983
HpySE526I ACGT 4 cut(s) 73, 1254, 1558, 1764
Hsp92I GRCGYC 1 cut(s) 1558
HspAI GCGC 1 cut(s) 1730
KpnI GGTACC 1 cut(s) 1468
Kzo9I GATC 8 cut(s) 101, 363, 401, 993, 1405, 1747, 2173, 2329
LguI GCTCTTC 1 cut(s) 308
LmnI GCTCC 9 cut(s) 277, 660, 1573, 1773, 1819, 1831, 2104, 2481, 2512
Lsp1109I GCAGC 6 cut(s) 487, 617, 1934, 1957, 2521, 2543
LweI GCATC 4 cut(s) 849, 1020, 1213, 1248
MabI ACCWGGT 2 cut(s) 778, 1467
MaeII ACGT 4 cut(s) 73, 1254, 1558, 1764
MaeIII GTNAC 9 cut(s) 413, 880, 1035, 1112, 1454, 1881, 1961, 1986, 2599
MalI GATC 8 cut(s) 103, 365, 403, 995, 1407, 1749, 2175, 2331
MboI GATC 8 cut(s) 101, 363, 401, 993, 1405, 1747, 2173, 2329
MflI RGATCY 3 cut(s) 101, 401, 1405
MhlI GDGCHC 2 cut(s) 1770, 2486
MlyI GAGTC 2 cut(s) 542, 2554
MmeI TCCRAC 1 cut(s) 1051
Mph1103I ATGCAT 3 cut(s) 1064, 1601, 1643
MroXI GAANNNNTTC 1 cut(s) 2451
MseI TTAA 8 cut(s) 17, 51, 258, 864, 1065, 1602, 1779, 2184
MslI CAYNNNNRTG 4 cut(s) 764, 1763, 2400, 2649
MspA1I CMGCKG 1 cut(s) 2534
MspI CCGG 3 cut(s) 718, 1571, 2102
MspR9I CCNGG 7 cut(s) 719, 780, 1199, 1469, 1617, 1896, 2301
Mva1269I GAATGC 4 cut(s) 535, 607, 1601, 1641
MvaI CCWGG 6 cut(s) 780, 1199, 1469, 1617, 1896, 2301
MvnI CGCG 2 cut(s) 411, 1041
MwoI GCNNNNNNNGC 2 cut(s) 237, 2528
NciI CCSGG 1 cut(s) 719
NcoI CCATGG 1 cut(s) 1679
NdeII GATC 8 cut(s) 101, 363, 401, 993, 1405, 1747, 2173, 2329
NlaIV GGNNCC 3 cut(s) 403, 469, 1466
NmeAIII GCCGAG 1 cut(s) 681
NmuCI GTSAC 5 cut(s) 880, 1112, 1454, 1881, 1986
NsiI ATGCAT 3 cut(s) 1064, 1601, 1643
NspI RCATGY 3 cut(s) 240, 382, 1826
OliI CACNNNNGTG 1 cut(s) 1763
PaeI GCATGC 2 cut(s) 240, 1826
PagI TCATGA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 2189
PciI ACATGT 1 cut(s) 378
PciSI GCTCTTC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 4 cut(s) 535, 607, 1601, 1641
PdmI GAANNNNTTC 1 cut(s) 2451
PfeI GAWTC 5 cut(s) 118, 769, 1043, 2138, 2161
PflMI CCANNNNNTGG 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 1615
PkrI GCNGC 7 cut(s) 502, 607, 734, 1924, 1972, 2533, 2536
PleI GAGTC 2 cut(s) 542, 2553
PpsI GAGTC 2 cut(s) 542, 2553
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 1 cut(s) 378
PshAI GACNNNNGTC 1 cut(s) 1561
PshBI ATTAAT 2 cut(s) 1065, 1779
PsiI TTATAA 1 cut(s) 2079
Psp6I CCWGG 6 cut(s) 778, 1197, 1467, 1615, 1894, 2299
PspFI CCCAGC 2 cut(s) 1425, 2366
PspGI CCWGG 6 cut(s) 778, 1197, 1467, 1615, 1894, 2299
PspN4I GGNNCC 3 cut(s) 403, 469, 1466
PspPI GGNCC 5 cut(s) 720, 782, 1619, 1676, 2041
PsrI GAACNNNNNNTAC 2 cut(s) 1736, 1768
PstI CTGCAG 2 cut(s) 938, 1975
PsuI RGATCY 3 cut(s) 101, 401, 1405
PvuII CAGCTG 1 cut(s) 2534
RsaI GTAC 4 cut(s) 905, 1288, 1466, 1981
RsaNI GTAC 4 cut(s) 904, 1287, 1465, 1980
RseI CAYNNNNRTG 4 cut(s) 764, 1763, 2400, 2649
SalI GTCGAC 2 cut(s) 4, 1472
SapI GCTCTTC 1 cut(s) 308
SaqAI TTAA 8 cut(s) 17, 51, 258, 864, 1065, 1602, 1779, 2184
SatI GCNGC 7 cut(s) 501, 606, 733, 1923, 1971, 2532, 2535
Sau3AI GATC 8 cut(s) 101, 363, 401, 993, 1405, 1747, 2173, 2329
Sau96I GGNCC 5 cut(s) 720, 782, 1619, 1676, 2041
SchI GAGTC 2 cut(s) 542, 2554
ScrFI CCNGG 7 cut(s) 719, 780, 1199, 1469, 1617, 1896, 2301
SduI GDGCHC 2 cut(s) 1770, 2486
SexAI ACCWGGT 2 cut(s) 778, 1467
SfaNI GCATC 4 cut(s) 849, 1020, 1213, 1248
SfcI CTRYAG 5 cut(s) 181, 694, 934, 1971, 2092
SinI GGWCC 4 cut(s) 782, 1619, 1676, 2041
SmiMI CAYNNNNRTG 4 cut(s) 764, 1763, 2400, 2649
SmlI CTYRAG 2 cut(s) 1103, 2584
SmoI CTYRAG 2 cut(s) 1103, 2584
SpeI ACTAGT 3 cut(s) 1171, 1399, 2449
SphI GCATGC 2 cut(s) 240, 1826
SseBI AGGCCT 1 cut(s) 2189
SsiI CCGC 2 cut(s) 411, 732
StuI AGGCCT 1 cut(s) 2189
StyD4I CCNGG 7 cut(s) 717, 778, 1197, 1467, 1615, 1894, 2299
StyI CCWWGG 7 cut(s) 727, 1364, 1679, 1839, 1949, 2044, 2590
TaaI ACNGT 6 cut(s) 328, 1000, 1629, 1816, 2145, 2605
TaiI ACGT 4 cut(s) 76, 1257, 1561, 1767
TaqI TCGA 3 cut(s) 5, 1280, 1473
TatI WGTACW 1 cut(s) 903
TauI GCSGC 1 cut(s) 735
TfiI GAWTC 5 cut(s) 118, 769, 1043, 2138, 2161
Tru1I TTAA 8 cut(s) 17, 51, 258, 864, 1065, 1602, 1779, 2184
Tru9I TTAA 8 cut(s) 17, 51, 258, 864, 1065, 1602, 1779, 2184
TscAI CASTG 7 cut(s) 919, 1005, 1233, 1727, 1932, 1949, 2628
TseFI GTSAC 5 cut(s) 880, 1112, 1454, 1881, 1986
TseI GCWGC 6 cut(s) 500, 605, 1922, 1970, 2531, 2534
Tsp45I GTSAC 5 cut(s) 880, 1112, 1454, 1881, 1986
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 7 cut(s) 919, 1005, 1233, 1727, 1932, 1949, 2628
Van91I CCANNNNNTGG 1 cut(s) 221
VpaK11BI GGWCC 4 cut(s) 782, 1619, 1676, 2041
VspI ATTAAT 2 cut(s) 1065, 1779
XapI RAATTY 8 cut(s) 293, 793, 1330, 1381, 1438, 1506, 1660, 2261
XbaI TCTAGA 2 cut(s) 104, 421
XceI RCATGY 3 cut(s) 240, 382, 1826
XmaJI CCTAGG 1 cut(s) 1364
XmiI GTMKAC 2 cut(s) 5, 1473
XmnI GAANNNNTTC 1 cut(s) 2451
ZraI GACGTC 1 cut(s) 1559
Zsp2I ATGCAT 3 cut(s) 1064, 1601, 1643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.