FvH4_4g13080

gene silencing by RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001623
Physical Location & Seq
Reverse (-)
16698521 .. 16701187
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13080.t1

Sequence Viewer

Length: 2667 bp
ATGTCGACAAAGGGGTTGATTGTAGCCAGAGAAAAACCTTTTCAGCAAACTAAGAACGTATCTACTCCAATGCGTGTTCCCATGAGGAGGCCTGGCCTTGGATCTAGAGGCCAAAGGATTCCACTTCTTACCAACCATTTCCAGGTAGGAATGAACAAATCCAAAGATGACTTTTACCACTATAGTATTGCTATGCAATATGATGATGGTACTCCAGTTGATGGGAAATGTGTTGGACGCAAAGTTCTTGATAAAGTTAAAGAGGTTTACGGCAAGGAGCTAGCATACAAAGAATTTGCTTATGATGGAGAGAAGACCTTGTTTACTGGTGGACAACTCCCTCATAAAAGACTTGAATTCATGATAGTACTGGATGACATATCAGCGAGCACGTCTGGGGGCTCTGGACGCCAAGAGAGGGAGGGTAATAGGAAGAGAGTAAAGCAGTTCTTTCGATCCAAAAAAATAAAGGTGCAGTTCAACTTGGCTGCTATAATCCCAATGCAAGCTATTGCGAACGTCTTACGAGGTCAGAACTCTGGCCAGTTTCAGGAAGCAGTTAGAGTTCTTGACATTATCCTAAGGCAGAATGCAGCAAAGCAGGGTTGTCTCCTTGTTCGACAGTCTTTCTTCCATAACAGCCCGAGGAACTTCATAGAGGTTGGAGGAGGCGTGTTAGGCTGTAGGGGTTTCCATTCAAGCTTCCAGGCCACGCAAGGCGGCCTGTCTCTCAATATGGATGTATCCACTACAATGATTCTAAAGCCTGGTCCTGTTGTGAATTTCCTCATGGAGAACCAACATGTCAGAGATCCTTACCAGATTGATTGGTACAAGGCTAAGAGGATGCTGAAAAATCTGAAGATTGTGACCTATGCCTCCAAGAAGGAGTACAAAATCACTGGATTGAGTGATAAACCCTGCAGACAGCAGATGTTCTTTTTGAAAGCAAAGCGAGGAGACTATGGAGATGGAGTAGAGATCTCAGTTTATGAATATTATGCTCAGTATAAGCAGATGCCGTTACGCGATTCAGCAAACTTTCCATGCATTAATGTTGGAAAACCAAAGAAACCAACCTACTTTCCTCTTGAGCTTTGTGAATTGGTTTCTTTGCAACGCTATACCAAGTCCTTATCTAATGTCCAAAGGGTTTCACTAGTGGAGAAGTCTAGGCAGAAGCCGCCGGAGCGAATGTCAACTTTGCGTGATGGACTGAGGAGTAGTAAGTATGAGGAAGACATGATGCTTCGGTCTGCTGGAATATCGATTAGTACAGAGCTCATGCCGGTTGAAGGTCGTGTGTTGTCAGCTCCAAAGTTGAGAGTGGGAAATGGGGATGTCATTTTTCCTAGGAATGGCAAATGGAATTTCAACAATAAAACGCTAGTACATCCTGTAAAACTAGAACGGTGGGCTGTTGTGAACTTCTCTGCTCGCTGCGACATTCCGTACCTGGTGGACACTATGATGAGGTGTGCAGACATGAAAGGAATTTCCATGGATCCTCCATTTGATGTGTTTGAAGAGAATGTTCAGTACAGACGTCAGTCAGCTCCGGTTAGAGTAGAAAAGATGTTCGAATACGTTAAGTCCAAACTTCCAAGGCCACCGCAGTTATTGTTGTGCATTCTTCCAGAGAGGAAGAATTCCGAGCTATATGGTCCATGGAAAAGGAAGAACCTTTCGGACAATGGGATTGCAACACAGTGCATTGCACCTACGAAAGTGAACGATCAATACATCACCAATGTGCTTCTGAAGATAAATGCAAAGCTAGGTGGAATGAACTCCTTGCTAACTGTGGAGCATGTTCCTTCTCTACCCTTGGTTTCCAAGTCCCCCACTATGATATTAGGGATGGATGTGTCACATGGCTCACCTGGGCAGTCATATATGCCTTCTATTGCAGCAGCGGTCAGTTCTAGGCACTGGCCTTTGATTTCTCGTTACCGAGCTGCCGTTCGTACTCAGTCACCAAAAACCGAGATAATTGCTAATCTTTTCAATCCAGTGTCTGATAAAGTGGACAAAGGCATAGTCAACGAACTTTTGCTAGACTTCTACAACACTTCAAACCACAGGAAACCAGAGCAGATTATTATCTTCAGGGATGGAGTGAGTGAATCAGAGTTCAACCAAGTCTTGAATCTGGAATTGGATCAGATTATTAAAGCCTGCAAGTTCATTGAAGAGAGCTGGTCGCCGAAGTTCATGGTTATTATTGCACAAAAGAGACATCATACAAAGTTCTTTCAGGCTGGTGGTTCAGCAGATAATGTTCCCCCCGGGACTATCGTCGACAACAAAGTCTGTGATCCAAAGAACAACGATTTCTACATGTGTTCTCATGCTGGGACGCTTGGAACAAGTCGGCCTACTCATTATCATGTTCTATATGATGAACTTGGCTTTTCAGCTGATGACTTGCAAGAACTTGTGCATTCTTTGTCATATGTTTATCAAAGGAGCAGCACGGCCGTGTCTCTAGTTGCCCCAGTATGCTATGCCCATCTAGCAGCAGCACAGATGAGTCAGTTCATCAAGTTTGATGAAATGTCAGAGACTGGCTCAAGCCGAGGTGGTGTTACTTGTGCTGGACCTGTGCCAGTGCAGGAAATACCTAGGCTACATAAAAACGTCGTCAATTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

889

Amino Acids

100.23

Weight (kDa)

9.48

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 38 - 194 3.9e-23 N-terminal domain of argonaute
ArgoL1 PF08699 206 - 254 4.8e-16 Argonaute linker 1 domain
PAZ PF02170 261 - 387 5.8e-25 PAZ domain
ArgoL2 PF16488 397 - 442 8.2e-10 Argonaute linker 2 domain
Piwi PF02171 541 - 849 8.6e-103 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2309
AatII GACGTC 1 cut(s) 1549
AccB7I CCANNNNNTGG 1 cut(s) 221
AccI GTMKAC 2 cut(s) 5, 2301
AccII CGCG 1 cut(s) 1029
AciI CCGC 4 cut(s) 720, 1184, 1613, 1916
AclWI GGATC 7 cut(s) 109, 450, 806, 1499, 1512, 2169, 2312
AcoI YGGCCR 2 cut(s) 541, 2478
AcsI RAATTY 6 cut(s) 293, 356, 781, 1369, 1494, 1648
AcuI CTGAAG 3 cut(s) 881, 1781, 2092
AcyI GRCGYC 2 cut(s) 409, 1546
AfaI GTAC 9 cut(s) 211, 369, 833, 893, 1276, 1392, 1454, 1541, 1969
AfiI CCNNNNNNNGG 8 cut(s) 87, 98, 142, 221, 418, 550, 1295, 1358
AflIII ACRYGT 2 cut(s) 802, 2340
AhlI ACTAGT 1 cut(s) 1159
AjiI CACGTC 1 cut(s) 393
AjnI CCWGG 6 cut(s) 91, 141, 705, 766, 1455, 1882
AjuI GAANNNNNNNTTGG 2 cut(s) 2141, 2173
AleI CACNNNNGTG 2 cut(s) 1751, 2480
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
Alw21I GWGCWC 2 cut(s) 392, 1284
Alw26I GTCTC 6 cut(s) 614, 732, 954, 2230, 2490, 2558
AlwI GGATC 7 cut(s) 109, 450, 806, 1499, 1512, 2169, 2312
AlwNI CAGNNNCTG 2 cut(s) 2018, 2567
Ama87I CYCGRG 2 cut(s) 643, 2288
ApeKI GCWGC 9 cut(s) 488, 593, 1440, 1910, 1913, 1958, 2472, 2519, 2522
ApoI RAATTY 6 cut(s) 293, 356, 781, 1369, 1494, 1648
ArsI GACNNNNNNTTYG 2 cut(s) 2025, 2057
AseI ATTAAT 1 cut(s) 1053
AspA2I CCTAGG 2 cut(s) 1352, 2624
AspS9I GGNCC 3 cut(s) 770, 1664, 2600
AsuC2I CCSGG 2 cut(s) 2289, 2290
AsuHPI GGTGA 3 cut(s) 1738, 1872, 1968
AsuII TTCGAA 1 cut(s) 1581
AsuNHI GCTAGC 1 cut(s) 280
AvaI CYCGRG 2 cut(s) 643, 2288
AvaII GGWCC 3 cut(s) 770, 1664, 2600
AvrII CCTAGG 2 cut(s) 1352, 2624
AxyI CCTNAGG 1 cut(s) 581
BaeI ACNNNNGTAYC 2 cut(s) 201, 234
BalI TGGCCA 1 cut(s) 543
BamHI GGATCC 1 cut(s) 1504
BanII GRGCYC 2 cut(s) 404, 1284
BbsI GAAGAC 2 cut(s) 320, 1245
Bbv12I GWGCWC 2 cut(s) 392, 1284
BbvI GCAGC 9 cut(s) 475, 605, 1427, 1922, 1925, 1945, 2484, 2531, 2534
BccI CCATC 8 cut(s) 200, 215, 299, 965, 1205, 1855, 2108, 2520
BceAI ACGGC 5 cut(s) 286, 1006, 1946, 2465, 2493
BcgI CGANNNNNNTGC 4 cut(s) 434, 468, 1976, 2010
BciT130I CCWGG 6 cut(s) 93, 143, 707, 768, 1457, 1884
BciVI GTATCC 1 cut(s) 754
BcnI CCSGG 2 cut(s) 2289, 2290
BcoDI GTCTC 6 cut(s) 614, 732, 954, 2230, 2490, 2558
BcuI ACTAGT 1 cut(s) 1159
BfmI CTRYAG 3 cut(s) 181, 682, 922
BfuI GTATCC 1 cut(s) 754
BglII AGATCT 1 cut(s) 981
BlnI CCTAGG 2 cut(s) 1352, 2624
BmcAI AGTACT 1 cut(s) 369
Bme1390I CCNGG 8 cut(s) 93, 143, 707, 768, 1457, 1884, 2289, 2290
Bme18I GGWCC 3 cut(s) 770, 1664, 2600
BmeT110I CYCGRG 2 cut(s) 643, 2288
BmgBI CACGTC 1 cut(s) 393
BmgT120I GGNCC 3 cut(s) 770, 1664, 2600
BmiI GGNNCC 1 cut(s) 1506
BmrFI CCNGG 8 cut(s) 93, 143, 707, 768, 1457, 1884, 2289, 2290
BmrI ACTGGG 1 cut(s) 2492
BmsI GCATC 3 cut(s) 837, 1008, 1236
BmtI GCTAGC 1 cut(s) 284
BmuI ACTGGG 1 cut(s) 2492
BoxI GACNNNNGTC 2 cut(s) 1549, 2297
BpiI GAAGAC 2 cut(s) 320, 1245
BplI GAGNNNNNCTC 2 cut(s) 2555, 2587
BpmI CTGGAG 1 cut(s) 198
Bpu14I TTCGAA 1 cut(s) 1581
BpuEI CTTGAG 2 cut(s) 1112, 2557
BpuMI CCSGG 2 cut(s) 2289, 2290
Bsa29I ATCGAT 1 cut(s) 1268
BsaBI GATNNNNATC 1 cut(s) 2102
BsaHI GRCGYC 2 cut(s) 409, 1546
BsaWI WCCGGW 1 cut(s) 1558
BsaXI ACNNNNNCTCC 6 cut(s) 657, 687, 960, 990, 1181, 1211
Bsc4I CCNNNNNNNGG 8 cut(s) 87, 98, 142, 221, 418, 550, 1295, 1358
Bse118I RCCGGY 1 cut(s) 1288
Bse21I CCTNAGG 1 cut(s) 581
Bse3DI GCAATG 1 cut(s) 1713
Bse8I GATNNNNATC 1 cut(s) 2102
BseBI CCWGG 6 cut(s) 93, 143, 707, 768, 1457, 1884
BseCI ATCGAT 1 cut(s) 1268
BseGI GGATG 8 cut(s) 379, 745, 852, 1345, 1393, 1866, 1870, 2119
BseJI GATNNNNATC 1 cut(s) 2102
BseLI CCNNNNNNNGG 8 cut(s) 87, 98, 142, 221, 418, 550, 1295, 1358
BseMI GCAATG 1 cut(s) 1713
BseMII CTCAG 4 cut(s) 999, 1019, 1208, 1985
BseRI GAGGAG 4 cut(s) 100, 681, 972, 1234
BseX3I CGGCCG 1 cut(s) 2478
BseXI GCAGC 9 cut(s) 475, 605, 1427, 1922, 1925, 1945, 2484, 2531, 2534
BseYI CCCAGC 1 cut(s) 2354
BsgI GTGCAG 3 cut(s) 494, 1500, 2633
Bsh1236I CGCG 1 cut(s) 1029
Bsh1285I CGRYCG 1 cut(s) 2481
BshVI ATCGAT 1 cut(s) 1268
BsiEI CGRYCG 1 cut(s) 2481
BsiHKAI GWGCWC 2 cut(s) 392, 1284
BsiHKCI CYCGRG 2 cut(s) 643, 2288
BsiSI CCGG 4 cut(s) 1187, 1289, 1559, 2289
BslFI GGGAC 3 cut(s) 1825, 2305, 2371
BslI CCNNNNNNNGG 8 cut(s) 87, 98, 142, 221, 418, 550, 1295, 1358
BsmAI GTCTC 6 cut(s) 614, 732, 954, 2230, 2490, 2558
BsmFI GGGAC 3 cut(s) 1825, 2305, 2371
BsmI GAATGC 3 cut(s) 595, 1629, 2443
BsoBI CYCGRG 2 cut(s) 643, 2288
Bsp119I TTCGAA 1 cut(s) 1581
Bsp1286I GDGCHC 3 cut(s) 392, 404, 1284
Bsp143I GATC 8 cut(s) 101, 455, 811, 981, 1504, 1735, 2161, 2317
Bsp19I CCATGG 2 cut(s) 1500, 1667
BspACI CCGC 4 cut(s) 720, 1184, 1613, 1916
BspCNI CTCAG 4 cut(s) 998, 1018, 1209, 1984
BspDI ATCGAT 1 cut(s) 1268
BspFNI CGCG 1 cut(s) 1029
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 1 cut(s) 1506
BspMAI CTGCAG 1 cut(s) 926
BspOI GCTAGC 1 cut(s) 284
BspPI GGATC 7 cut(s) 109, 450, 806, 1499, 1512, 2169, 2312
BspT104I TTCGAA 1 cut(s) 1581
BsrDI GCAATG 1 cut(s) 1713
BsrFI RCCGGY 1 cut(s) 1288
BssAI RCCGGY 1 cut(s) 1288
BssMI GATC 8 cut(s) 101, 455, 811, 981, 1504, 1735, 2161, 2317
BssNI GRCGYC 2 cut(s) 409, 1546
BssT1I CCWWGG 7 cut(s) 97, 1352, 1500, 1604, 1667, 1827, 2624
Bst2UI CCWGG 6 cut(s) 93, 143, 707, 768, 1457, 1884
Bst4CI ACNGT 4 cut(s) 624, 1413, 1710, 1804
Bst6I CTCTTC 3 cut(s) 428, 1521, 2187
BstACI GRCGYC 2 cut(s) 409, 1546
BstBI TTCGAA 1 cut(s) 1581
BstC8I GCNNGC 5 cut(s) 282, 388, 507, 1438, 2179
BstDEI CTNAG 7 cut(s) 51, 581, 840, 985, 1005, 1217, 1971
BstDSI CCRYGG 2 cut(s) 1500, 1667
BstF5I GGATG 8 cut(s) 379, 745, 852, 1345, 1393, 1866, 1870, 2119
BstFNI CGCG 1 cut(s) 1029
BstKTI GATC 8 cut(s) 104, 458, 814, 984, 1507, 1738, 2164, 2320
BstMAI GTCTC 6 cut(s) 614, 732, 954, 2230, 2490, 2558
BstMBI GATC 8 cut(s) 101, 455, 811, 981, 1504, 1735, 2161, 2317
BstMCI CGRYCG 1 cut(s) 2481
BstMWI GCNNNNNNNGC 5 cut(s) 408, 678, 1183, 1189, 2516
BstNI CCWGG 6 cut(s) 93, 143, 707, 768, 1457, 1884
BstNSI RCATGY 3 cut(s) 806, 1814, 2344
BstPAI GACNNNNGTC 2 cut(s) 1549, 2297
BstSCI CCNGG 8 cut(s) 91, 141, 705, 766, 1455, 1882, 2287, 2288
BstSFI CTRYAG 3 cut(s) 181, 682, 922
BstUI CGCG 1 cut(s) 1029
BstV1I GCAGC 9 cut(s) 475, 605, 1427, 1922, 1925, 1945, 2484, 2531, 2534
BstV2I GAAGAC 2 cut(s) 320, 1245
BstX2I RGATCY 4 cut(s) 101, 811, 981, 1504
BstYI RGATCY 4 cut(s) 101, 811, 981, 1504
BstZI CGGCCG 1 cut(s) 2478
Bsu15I ATCGAT 1 cut(s) 1268
Bsu36I CCTNAGG 1 cut(s) 581
BsuI GTATCC 1 cut(s) 754
BsuTUI ATCGAT 1 cut(s) 1268
BtgI CCRYGG 2 cut(s) 1500, 1667
BtrI CACGTC 1 cut(s) 393
BtsCI GGATG 8 cut(s) 379, 745, 852, 1345, 1393, 1866, 1870, 2119
BtsIMutI CAGTG 5 cut(s) 900, 1715, 1930, 2019, 2616
Cac8I GCNNGC 5 cut(s) 282, 388, 507, 1438, 2179
CaiI CAGNNNCTG 2 cut(s) 2018, 2567
CciI TCATGA 1 cut(s) 360
Cfr10I RCCGGY 1 cut(s) 1288
Cfr13I GGNCC 3 cut(s) 770, 1664, 2600
Cfr9I CCCGGG 1 cut(s) 2288
ClaI ATCGAT 1 cut(s) 1268
CseI GACGC 3 cut(s) 246, 417, 2368
CsiI ACCWGGT 1 cut(s) 1455
Csp6I GTAC 9 cut(s) 210, 368, 832, 892, 1275, 1391, 1453, 1540, 1968
CviQI GTAC 9 cut(s) 210, 368, 832, 892, 1275, 1391, 1453, 1540, 1968
DdeI CTNAG 7 cut(s) 51, 581, 840, 985, 1005, 1217, 1971
DpnI GATC 8 cut(s) 103, 457, 813, 983, 1506, 1737, 2163, 2319
DpnII GATC 8 cut(s) 101, 455, 811, 981, 1504, 1735, 2161, 2317
DrdI GACNNNNNNGTC 1 cut(s) 2309
DseDI GACNNNNNNGTC 1 cut(s) 2309
EaeI YGGCCR 2 cut(s) 541, 2478
EagI CGGCCG 1 cut(s) 2478
Eam1104I CTCTTC 3 cut(s) 428, 1521, 2187
EarI CTCTTC 3 cut(s) 428, 1521, 2187
Ecl136II GAGCTC 1 cut(s) 1282
EclXI CGGCCG 1 cut(s) 2478
Eco130I CCWWGG 7 cut(s) 97, 1352, 1500, 1604, 1667, 1827, 2624
Eco147I AGGCCT 1 cut(s) 91
Eco24I GRGCYC 2 cut(s) 404, 1284
Eco47I GGWCC 3 cut(s) 770, 1664, 2600
Eco52I CGGCCG 1 cut(s) 2478
Eco53kI GAGCTC 1 cut(s) 1282
Eco57I CTGAAG 3 cut(s) 881, 1781, 2092
Eco81I CCTNAGG 1 cut(s) 581
Eco88I CYCGRG 2 cut(s) 643, 2288
EcoICRI GAGCTC 1 cut(s) 1282
EcoRI GAATTC 2 cut(s) 356, 1648
EcoRII CCWGG 6 cut(s) 91, 141, 705, 766, 1455, 1882
EcoT14I CCWWGG 7 cut(s) 97, 1352, 1500, 1604, 1667, 1827, 2624
EcoT22I ATGCAT 1 cut(s) 1052
EcoT38I GRGCYC 2 cut(s) 404, 1284
ErhI CCWWGG 7 cut(s) 97, 1352, 1500, 1604, 1667, 1827, 2624
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 3 cut(s) 1825, 2305, 2371
FauNDI CATATG 1 cut(s) 2455
FblI GTMKAC 2 cut(s) 5, 2301
FokI GGATG 8 cut(s) 386, 752, 859, 1352, 1380, 1873, 1877, 2126
FriOI GRGCYC 2 cut(s) 404, 1284
GsaI CCCAGC 1 cut(s) 2358
GsuI CTGGAG 1 cut(s) 198
HapII CCGG 4 cut(s) 1187, 1289, 1559, 2289
HgaI GACGC 3 cut(s) 246, 417, 2368
Hin1I GRCGYC 2 cut(s) 409, 1546
HincII GTYRAC 4 cut(s) 6, 1200, 2044, 2302
HindII GTYRAC 4 cut(s) 6, 1200, 2044, 2302
HindIII AAGCTT 1 cut(s) 700
HinfI GANTC 6 cut(s) 118, 757, 1031, 2126, 2149, 2533
HpaII CCGG 4 cut(s) 1187, 1289, 1559, 2289
HphI GGTGA 3 cut(s) 1738, 1872, 1968
Hpy99I CGWCG 2 cut(s) 2303, 2645
HpyAV CCTTC 4 cut(s) 880, 1289, 1827, 1911
HpyCH4III ACNGT 4 cut(s) 624, 1413, 1710, 1804
HpyCH4IV ACGT 6 cut(s) 57, 392, 519, 1546, 1587, 2640
HpyF10VI GCNNNNNNNGC 5 cut(s) 408, 678, 1183, 1189, 2516
HpyF3I CTNAG 7 cut(s) 51, 581, 840, 985, 1005, 1217, 1971
HpySE526I ACGT 6 cut(s) 57, 392, 519, 1546, 1587, 2640
Hsp92I GRCGYC 2 cut(s) 409, 1546
Kzo9I GATC 8 cut(s) 101, 455, 811, 981, 1504, 1735, 2161, 2317
LmnI GCTCC 6 cut(s) 277, 1189, 1318, 1561, 1807, 2469
Lsp1109I GCAGC 9 cut(s) 475, 605, 1427, 1922, 1925, 1945, 2484, 2531, 2534
LweI GCATC 3 cut(s) 837, 1008, 1236
MabI ACCWGGT 1 cut(s) 1455
MaeII ACGT 6 cut(s) 57, 392, 519, 1546, 1587, 2640
MaeIII GTNAC 6 cut(s) 868, 1023, 1869, 1949, 1974, 2587
MalI GATC 8 cut(s) 103, 457, 813, 983, 1506, 1737, 2163, 2319
MboI GATC 8 cut(s) 101, 455, 811, 981, 1504, 1735, 2161, 2317
MflI RGATCY 4 cut(s) 101, 811, 981, 1504
MhlI GDGCHC 3 cut(s) 392, 404, 1284
MlsI TGGCCA 1 cut(s) 543
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 1 cut(s) 2542
MmeI TCCRAC 3 cut(s) 214, 643, 1039
Mox20I TGGCCA 1 cut(s) 543
Mph1103I ATGCAT 1 cut(s) 1052
MscI TGGCCA 1 cut(s) 543
MseI TTAA 4 cut(s) 258, 1053, 1590, 2172
MslI CAYNNNNRTG 5 cut(s) 752, 1469, 1751, 2388, 2480
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 2 cut(s) 1916, 2420
MspI CCGG 4 cut(s) 1187, 1289, 1559, 2289
MspR9I CCNGG 8 cut(s) 93, 143, 707, 768, 1457, 1884, 2289, 2290
Mva1269I GAATGC 3 cut(s) 595, 1629, 2443
MvaI CCWGG 6 cut(s) 93, 143, 707, 768, 1457, 1884
MvnI CGCG 1 cut(s) 1029
MwoI GCNNNNNNNGC 5 cut(s) 408, 678, 1183, 1189, 2516
NciI CCSGG 2 cut(s) 2289, 2290
NcoI CCATGG 2 cut(s) 1500, 1667
NdeI CATATG 1 cut(s) 2455
NdeII GATC 8 cut(s) 101, 455, 811, 981, 1504, 1735, 2161, 2317
NheI GCTAGC 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 1506
NmeAIII GCCGAG 1 cut(s) 2603
NmuCI GTSAC 3 cut(s) 868, 1869, 1974
NsiI ATGCAT 1 cut(s) 1052
NspI RCATGY 3 cut(s) 806, 1814, 2344
NspV TTCGAA 1 cut(s) 1581
OliI CACNNNNGTG 2 cut(s) 1751, 2480
PagI TCATGA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 91
PciI ACATGT 2 cut(s) 802, 2340
PctI GAATGC 3 cut(s) 595, 1629, 2443
PfeI GAWTC 5 cut(s) 118, 757, 1031, 2126, 2149
PflMI CCANNNNNTGG 1 cut(s) 221
PleI GAGTC 1 cut(s) 2541
PpsI GAGTC 1 cut(s) 2541
PscI ACATGT 2 cut(s) 802, 2340
PshAI GACNNNNGTC 2 cut(s) 1549, 2297
PshBI ATTAAT 1 cut(s) 1053
Psp124BI GAGCTC 1 cut(s) 1284
Psp6I CCWGG 6 cut(s) 91, 141, 705, 766, 1455, 1882
PspFI CCCAGC 1 cut(s) 2354
PspGI CCWGG 6 cut(s) 91, 141, 705, 766, 1455, 1882
PspN4I GGNNCC 1 cut(s) 1506
PspPI GGNCC 3 cut(s) 770, 1664, 2600
PsrI GAACNNNNNNTAC 2 cut(s) 1724, 1756
PstI CTGCAG 1 cut(s) 926
PstNI CAGNNNCTG 2 cut(s) 2018, 2567
PsuI RGATCY 4 cut(s) 101, 811, 981, 1504
PvuII CAGCTG 1 cut(s) 2420
RsaI GTAC 9 cut(s) 211, 369, 833, 893, 1276, 1392, 1454, 1541, 1969
RsaNI GTAC 9 cut(s) 210, 368, 832, 892, 1275, 1391, 1453, 1540, 1968
RseI CAYNNNNRTG 5 cut(s) 752, 1469, 1751, 2388, 2480
SacI GAGCTC 1 cut(s) 1284
SalI GTCGAC 2 cut(s) 4, 2300
SaqAI TTAA 4 cut(s) 258, 1053, 1590, 2172
Sau3AI GATC 8 cut(s) 101, 455, 811, 981, 1504, 1735, 2161, 2317
Sau96I GGNCC 3 cut(s) 770, 1664, 2600
ScaI AGTACT 1 cut(s) 369
SchI GAGTC 1 cut(s) 2542
ScrFI CCNGG 8 cut(s) 93, 143, 707, 768, 1457, 1884, 2289, 2290
SduI GDGCHC 3 cut(s) 392, 404, 1284
SexAI ACCWGGT 1 cut(s) 1455
SfaNI GCATC 3 cut(s) 837, 1008, 1236
SfcI CTRYAG 3 cut(s) 181, 682, 922
SfuI TTCGAA 1 cut(s) 1581
SinI GGWCC 3 cut(s) 770, 1664, 2600
SmaI CCCGGG 1 cut(s) 2290
SmiMI CAYNNNNRTG 5 cut(s) 752, 1469, 1751, 2388, 2480
SmlI CTYRAG 2 cut(s) 1091, 2572
SmoI CTYRAG 2 cut(s) 1091, 2572
SpeI ACTAGT 1 cut(s) 1159
SseBI AGGCCT 1 cut(s) 91
SsiI CCGC 4 cut(s) 720, 1184, 1613, 1916
SspI AATATT 1 cut(s) 998
SstI GAGCTC 1 cut(s) 1284
StuI AGGCCT 1 cut(s) 91
StyD4I CCNGG 8 cut(s) 91, 141, 705, 766, 1455, 1882, 2287, 2288
StyI CCWWGG 7 cut(s) 97, 1352, 1500, 1604, 1667, 1827, 2624
TaaI ACNGT 4 cut(s) 624, 1413, 1710, 1804
TaiI ACGT 6 cut(s) 60, 395, 522, 1549, 1590, 2643
TaqI TCGA 6 cut(s) 5, 454, 619, 1268, 1581, 2301
TaqII GACCGA 1 cut(s) 1242
TatI WGTACW 5 cut(s) 367, 891, 1274, 1390, 1539
TauI GCSGC 2 cut(s) 723, 1186
TfiI GAWTC 5 cut(s) 118, 757, 1031, 2126, 2149
Tru1I TTAA 4 cut(s) 258, 1053, 1590, 2172
Tru9I TTAA 4 cut(s) 258, 1053, 1590, 2172
TscAI CASTG 5 cut(s) 907, 1715, 1937, 2019, 2616
TseFI GTSAC 3 cut(s) 868, 1869, 1974
TseI GCWGC 9 cut(s) 488, 593, 1440, 1910, 1913, 1958, 2472, 2519, 2522
Tsp45I GTSAC 3 cut(s) 868, 1869, 1974
TspGWI ACGGA 1 cut(s) 1440
TspMI CCCGGG 1 cut(s) 2288
TspRI CASTG 5 cut(s) 907, 1715, 1937, 2019, 2616
Van91I CCANNNNNTGG 1 cut(s) 221
VpaK11BI GGWCC 3 cut(s) 770, 1664, 2600
VspI ATTAAT 1 cut(s) 1053
XapI RAATTY 6 cut(s) 293, 356, 781, 1369, 1494, 1648
XbaI TCTAGA 1 cut(s) 104
XceI RCATGY 3 cut(s) 806, 1814, 2344
XmaI CCCGGG 1 cut(s) 2288
XmaJI CCTAGG 2 cut(s) 1352, 2624
XmiI GTMKAC 2 cut(s) 5, 2301
ZraI GACGTC 1 cut(s) 1547
ZrmI AGTACT 1 cut(s) 369
Zsp2I ATGCAT 1 cut(s) 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.