Rh1BG084900

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001623
Physical Location & Seq
Forward (+)
13409508 .. 13419488
9981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG084900.1

Sequence Viewer

Length: 1140 bp
ATGATGCTTCATTCTTCTGGAATATCGATTGGTACAGAATTTATGCAGGTTGAAGGCCGTGTTTTGTCAGCCCCAAGGTTGAAAGTGGGAAATGGAGAGGATTTTTCTCCTCGGAATGGGAGATGGAATTTCAACAATAAGAAATTAGTAGAACCTGTAAAACTAGAGCACTGGGCTGTTGTAAATTTCTCTGCTCGCTGTGACGTTCGCTTCCTGGTCAACACTATGATAAAGTGTGGAGGCATGAAAGGAATTTCCATCAACCCTCCGTTTGATGTGTTTGAAGAGAGCATTCAGAATAGACGTGAGTCAGCTCTGAGTAGAGTAGAAAAGATGTTTGAATATATTAAGTCCAAACTTCCGGGACCACCTCAGTTTTTGTTGTGCATTCTTCCAGAGAGGAAGAATTCTGACATATATGGTCCATGGAAAAGGAAAAACCTTTCAGACGTTGGGATTGTAACACAGTGTATTGCACCAACTAAAGTGAATGATCAATACATCACAAATGTGCTCCTGAAAATTAATACAAAGCTTGATGGAATCAACTCCTTGCTAATGGTGGAGCATTCTCCTTCTCTTCCCTTGGTTTCCAAGTCCCCCACTCTGATTTTAGGGATGGATGTGTCACATGGCTCACCTGGACGTTCCGATGTGCCTTCTATTGCAGCGTATTGCATATTCCACATTCTCTTAGAGATTGTTGCTGTACTATGTTACGAATTACATATTAAAAGGGGGAATTTTGTAGGTGGGACTATTATTGACAACAAAGTCTGTCATCCAAAGAACAATGATTTCTACTTATGTTCTCATGCTGGGATGATTGGAACAACTCGTCCTACTCATTATCATGTTCTATATGATGAACTTGGCTTCTCAGCGGATGACTTGCAAGAACTTGTTCATTCTTTATCATATGTTTACCAAAGGAGCACAACGGCCATATCTGTAGTTGCTCCGGTATGCTATGCCCATCTAGCAGCAGCACAGATGAGTCAGTTTATTAAGTTCGATGAAATATCAGAGACTGGTTCAAGCCAAGGTGGTTCTGGTGGTGTTACTGTTGCTGGAGCTGTGCCAGTGCAGGAGTTACCAAGGCTACATGAAAGTGTCGTCAACTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

379

Amino Acids

41.93

Weight (kDa)

7.61

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoL2 PF16488 4 - 28 1.1e-06 Argonaute linker 2 domain
ArgoMid PF16487 36 - 89 7.3e-07 Mid domain of argonaute
Piwi PF02171 127 - 226 2.1e-23 Piwi domain
Piwi PF02171 244 - 337 5.4e-37 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 773
Acc36I ACCTGC 1 cut(s) 37
AciI CCGC 1 cut(s) 884
AcoI YGGCCR 1 cut(s) 942
AcsI RAATTY 6 cut(s) 38, 127, 184, 252, 406, 742
AfaI GTAC 2 cut(s) 34, 711
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 6 cut(s) 53, 82, 133, 284, 341, 1038
AjiI CACGTC 1 cut(s) 305
AjnI CCWGG 2 cut(s) 213, 640
AleI CACNNNNGTG 1 cut(s) 509
AloI GAACNNNNNNTCC 2 cut(s) 823, 855
AluBI AGCT 3 cut(s) 314, 535, 1076
AluI AGCT 3 cut(s) 314, 535, 1076
Alw21I GWGCWC 3 cut(s) 171, 516, 938
Alw26I GTCTC 1 cut(s) 1022
AlwNI CAGNNNCTG 1 cut(s) 1031
AoxI GGCC 2 cut(s) 55, 942
ApeKI GCWGC 3 cut(s) 668, 983, 986
ApoI RAATTY 6 cut(s) 38, 127, 184, 252, 406, 742
AseI ATTAAT 1 cut(s) 525
Asp700I GAANNNNTTC 1 cut(s) 903
AspS9I GGNCC 2 cut(s) 365, 422
AsuC2I CCSGG 1 cut(s) 363
AsuHPI GGTGA 1 cut(s) 630
AvaII GGWCC 2 cut(s) 365, 422
Bbv12I GWGCWC 3 cut(s) 171, 516, 938
BbvI GCAGC 3 cut(s) 680, 995, 998
BccI CCATC 5 cut(s) 117, 266, 533, 613, 984
BceAI ACGGC 2 cut(s) 42, 957
BciT130I CCWGG 2 cut(s) 215, 642
BclI TGATCA 1 cut(s) 493
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 1022
BfaI CTAG 2 cut(s) 164, 980
BfmI CTRYAG 1 cut(s) 951
BfuAI ACCTGC 1 cut(s) 37
BisI GCNGC 3 cut(s) 669, 984, 987
BlsI GCNGC 3 cut(s) 670, 985, 988
Bme1390I CCNGG 3 cut(s) 215, 363, 642
Bme18I GGWCC 2 cut(s) 365, 422
BmgBI CACGTC 1 cut(s) 305
BmgT120I GGNCC 2 cut(s) 365, 422
BmiI GGNNCC 1 cut(s) 366
BmrFI CCNGG 3 cut(s) 215, 363, 642
BmrI ACTGGG 1 cut(s) 181
BmuI ACTGGG 1 cut(s) 181
BoxI GACNNNNGTC 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 1092
BpuMI CCSGG 1 cut(s) 363
Bsa29I ATCGAT 1 cut(s) 26
BsaJI CCNNGG 6 cut(s) 74, 110, 425, 585, 1042, 1097
BsaWI WCCGGW 1 cut(s) 961
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 3 cut(s) 176, 1036, 1082
BseBI CCWGG 2 cut(s) 215, 642
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 6 cut(s) 74, 110, 425, 585, 1042, 1097
BseGI GGATG 5 cut(s) 624, 628, 781, 828, 892
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 3 cut(s) 308, 386, 894
BseNI ACTGG 3 cut(s) 176, 1036, 1082
BseRI GAGGAG 1 cut(s) 99
BseXI GCAGC 3 cut(s) 680, 995, 998
BseYI CCCAGC 1 cut(s) 818
BsgI GTGCAG 1 cut(s) 1106
BshFI GGCC 2 cut(s) 57, 944
BshVI ATCGAT 1 cut(s) 26
BsiHKAI GWGCWC 3 cut(s) 171, 516, 938
BsiSI CCGG 2 cut(s) 362, 962
BslFI GGGAC 3 cut(s) 378, 583, 769
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 1022
BsmFI GGGAC 3 cut(s) 378, 583, 769
BsmI GAATGC 3 cut(s) 291, 387, 568
BsnI GGCC 2 cut(s) 57, 944
Bsp1286I GDGCHC 3 cut(s) 171, 516, 938
Bsp143I GATC 1 cut(s) 493
Bsp19I CCATGG 1 cut(s) 425
BspACI CCGC 1 cut(s) 884
BspANI GGCC 2 cut(s) 57, 944
BspCNI CTCAG 3 cut(s) 309, 385, 893
BspDI ATCGAT 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 366
BspMI ACCTGC 1 cut(s) 37
BsrI ACTGG 3 cut(s) 176, 1036, 1082
BssECI CCNNGG 6 cut(s) 74, 110, 425, 585, 1042, 1097
BssMI GATC 1 cut(s) 493
BssT1I CCWWGG 5 cut(s) 74, 425, 585, 1042, 1097
Bst2UI CCWGG 2 cut(s) 215, 642
Bst4CI ACNGT 2 cut(s) 468, 1066
Bst6I CTCTTC 2 cut(s) 279, 585
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 4 cut(s) 317, 372, 694, 880
BstDSI CCRYGG 1 cut(s) 425
BstF5I GGATG 5 cut(s) 624, 628, 781, 828, 892
BstKTI GATC 1 cut(s) 496
BstMAI GTCTC 1 cut(s) 1022
BstMBI GATC 1 cut(s) 493
BstMWI GCNNNNNNNGC 1 cut(s) 980
BstNI CCWGG 2 cut(s) 215, 642
BstPAI GACNNNNGTC 1 cut(s) 307
BstSCI CCNGG 3 cut(s) 213, 361, 640
BstSFI CTRYAG 1 cut(s) 951
BstV1I GCAGC 3 cut(s) 680, 995, 998
Bsu15I ATCGAT 1 cut(s) 26
BsuRI GGCC 2 cut(s) 57, 944
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 425
BtrI CACGTC 1 cut(s) 305
BtsCI GGATG 5 cut(s) 624, 628, 781, 828, 892
BtsIMutI CAGTG 3 cut(s) 169, 473, 1089
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 196
CaiI CAGNNNCTG 1 cut(s) 1031
Cfr13I GGNCC 2 cut(s) 365, 422
ClaI ATCGAT 1 cut(s) 26
Csp6I GTAC 2 cut(s) 33, 710
CviAII CATG 7 cut(s) 244, 426, 632, 815, 854, 1106, 1126
CviQI GTAC 2 cut(s) 33, 710
DdeI CTNAG 4 cut(s) 317, 372, 694, 880
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
DrdI GACNNNNNNGTC 1 cut(s) 773
DseDI GACNNNNNNGTC 1 cut(s) 773
EaeI YGGCCR 1 cut(s) 942
Eam1104I CTCTTC 2 cut(s) 279, 585
EarI CTCTTC 2 cut(s) 279, 585
Eco130I CCWWGG 5 cut(s) 74, 425, 585, 1042, 1097
Eco47I GGWCC 2 cut(s) 365, 422
EcoRI GAATTC 1 cut(s) 406
EcoRII CCWGG 2 cut(s) 213, 640
EcoT14I CCWWGG 5 cut(s) 74, 425, 585, 1042, 1097
ErhI CCWWGG 5 cut(s) 74, 425, 585, 1042, 1097
FaeI CATG 7 cut(s) 247, 429, 635, 818, 857, 1109, 1129
FaqI GGGAC 3 cut(s) 378, 583, 769
FatI CATG 7 cut(s) 243, 425, 631, 814, 853, 1105, 1125
FauNDI CATATG 1 cut(s) 919
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 3 cut(s) 669, 984, 987
FokI GGATG 5 cut(s) 631, 635, 768, 835, 899
Fsp4HI GCNGC 3 cut(s) 669, 984, 987
FspBI CTAG 2 cut(s) 164, 980
GluI GCNGC 3 cut(s) 669, 984, 987
GsaI CCCAGC 1 cut(s) 822
GsuI CTGGAG 1 cut(s) 1092
HaeIII GGCC 2 cut(s) 57, 944
HapII CCGG 2 cut(s) 362, 962
Hin1II CATG 7 cut(s) 247, 429, 635, 818, 857, 1109, 1129
HincII GTYRAC 2 cut(s) 220, 1120
HindII GTYRAC 2 cut(s) 220, 1120
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 3 cut(s) 308, 543, 997
HpaII CCGG 2 cut(s) 362, 962
HphI GGTGA 1 cut(s) 630
Hpy166II GTNNAC 3 cut(s) 220, 925, 1120
Hpy188I TCNGA 8 cut(s) 114, 297, 318, 412, 448, 609, 652, 1027
Hpy188III TCNNGA 3 cut(s) 18, 395, 517
Hpy8I GTNNAC 3 cut(s) 220, 925, 1120
HpyAV CCTTC 3 cut(s) 47, 585, 669
HpyCH4III ACNGT 2 cut(s) 468, 1066
HpyCH4IV ACGT 4 cut(s) 204, 304, 450, 646
HpyCH4V TGCA 7 cut(s) 46, 387, 476, 668, 678, 895, 1087
HpyF10VI GCNNNNNNNGC 1 cut(s) 980
HpyF3I CTNAG 4 cut(s) 317, 372, 694, 880
HpySE526I ACGT 4 cut(s) 204, 304, 450, 646
Hsp92II CATG 7 cut(s) 247, 429, 635, 818, 857, 1109, 1129
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 1 cut(s) 493
LmnI GCTCC 5 cut(s) 519, 565, 933, 964, 1073
Lsp1109I GCAGC 3 cut(s) 680, 995, 998
MaeI CTAG 2 cut(s) 164, 980
MaeII ACGT 4 cut(s) 204, 304, 450, 646
MaeIII GTNAC 6 cut(s) 200, 460, 627, 716, 1060, 1092
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 6 cut(s) 6, 296, 383, 415, 572, 1123
MhlI GDGCHC 3 cut(s) 171, 516, 938
MluCI AATT 9 cut(s) 38, 127, 143, 184, 252, 406, 522, 722, 742
MlyI GAGTC 2 cut(s) 317, 1006
MnlI CCTC 6 cut(s) 91, 120, 233, 276, 381, 393
MroXI GAANNNNTTC 1 cut(s) 903
MseI TTAA 4 cut(s) 348, 525, 732, 1008
MslI CAYNNNNRTG 3 cut(s) 509, 852, 1110
MspA1I CMGCKG 1 cut(s) 884
MspI CCGG 2 cut(s) 362, 962
MspR9I CCNGG 3 cut(s) 215, 363, 642
Mva1269I GAATGC 3 cut(s) 291, 387, 568
MvaI CCWGG 2 cut(s) 215, 642
MwoI GCNNNNNNNGC 1 cut(s) 980
NciI CCSGG 1 cut(s) 363
NcoI CCATGG 1 cut(s) 425
NdeI CATATG 1 cut(s) 919
NdeII GATC 1 cut(s) 493
NlaIII CATG 7 cut(s) 247, 429, 635, 818, 857, 1109, 1129
NlaIV GGNNCC 1 cut(s) 366
NmuCI GTSAC 2 cut(s) 200, 627
OliI CACNNNNGTG 1 cut(s) 509
PctI GAATGC 3 cut(s) 291, 387, 568
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 1 cut(s) 543
PfoI TCCNGGA 1 cut(s) 361
PkrI GCNGC 3 cut(s) 670, 985, 988
PleI GAGTC 2 cut(s) 316, 1005
PpsI GAGTC 2 cut(s) 316, 1005
PshAI GACNNNNGTC 1 cut(s) 307
PshBI ATTAAT 1 cut(s) 525
Psp6I CCWGG 2 cut(s) 213, 640
PspFI CCCAGC 1 cut(s) 818
PspGI CCWGG 2 cut(s) 213, 640
PspN4I GGNNCC 1 cut(s) 366
PspPI GGNCC 2 cut(s) 365, 422
PstNI CAGNNNCTG 1 cut(s) 1031
RsaI GTAC 2 cut(s) 34, 711
RsaNI GTAC 2 cut(s) 33, 710
RseI CAYNNNNRTG 3 cut(s) 509, 852, 1110
SaqAI TTAA 4 cut(s) 348, 525, 732, 1008
SatI GCNGC 3 cut(s) 669, 984, 987
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 2 cut(s) 365, 422
SchI GAGTC 2 cut(s) 317, 1006
ScrFI CCNGG 3 cut(s) 215, 363, 642
SduI GDGCHC 3 cut(s) 171, 516, 938
SfcI CTRYAG 1 cut(s) 951
SinI GGWCC 2 cut(s) 365, 422
SmiMI CAYNNNNRTG 3 cut(s) 509, 852, 1110
Sse9I AATT 9 cut(s) 38, 127, 143, 184, 252, 406, 522, 722, 742
SsiI CCGC 1 cut(s) 884
SspMI CTAG 2 cut(s) 164, 980
StyD4I CCNGG 3 cut(s) 213, 361, 640
StyI CCWWGG 5 cut(s) 74, 425, 585, 1042, 1097
TaaI ACNGT 2 cut(s) 468, 1066
TaiI ACGT 4 cut(s) 207, 307, 453, 649
TaqI TCGA 2 cut(s) 26, 1014
TasI AATT 9 cut(s) 38, 127, 143, 184, 252, 406, 522, 722, 742
TatI WGTACW 1 cut(s) 709
TfiI GAWTC 1 cut(s) 543
Tru1I TTAA 4 cut(s) 348, 525, 732, 1008
Tru9I TTAA 4 cut(s) 348, 525, 732, 1008
TscAI CASTG 3 cut(s) 176, 473, 1089
TseFI GTSAC 2 cut(s) 200, 627
TseI GCWGC 3 cut(s) 668, 983, 986
Tsp45I GTSAC 2 cut(s) 200, 627
TspDTI ATGAA 5 cut(s) 260, 882, 896, 1032, 1122
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 3 cut(s) 176, 473, 1089
VpaK11BI GGWCC 2 cut(s) 365, 422
VspI ATTAAT 1 cut(s) 525
XapI RAATTY 6 cut(s) 38, 127, 184, 252, 406, 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 1049
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 2 cut(s) 164, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.