pycom07g04070

gene silencing by RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001623
Physical Location & Seq
Forward (+)
3497644 .. 3500791
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g04070.3

Sequence Viewer

Length: 1194 bp
ATGAGCCTGGCAAAAGTAAGGAAAGCGAAAGATTGTAAATTTCAAAACGGAAATGATAACCGAGGCCCAAACGTGCTTTCCATGTGTATTCCTCCTGATGAGTTTGAATTTGATAGAGATGTACCTCTTTGTTACCTGCAGTTTACACCTACATTGTTTATCAATTGCGGGGTTGGCAGGTATAAGGTAATGGAGTCTTCCGATCCCAAAGGAGCTGAGCTTCCTCCTCCTCCCGCCATTATTCCTCCAAATGTTGTCCCAACTATGGCAGAAGGAAAATCTGTTGAAGTGTATAAGAAACCATCAACTTCTAAACTTGTTCCCATGTCAAGGCCAGGCTTTGGATCTAGAGGAAAAAGCATCCAACTAACAACTAACCATTTCAAAGTGGGAGTGAAAATTTCTGATGGCTACTTTTTCCATTATAGTATTGACATGTTTTATGAGGATGGAACCCCAGTTACAGCAAACGCTATTGGGCGCAAAGTTGTTGAAAAAGTTAAGGAGACATATGACAGGGATCTAGGACGAAAAGAATTTGCTTATGATGGACAGAAGAACTTGTTCACTGTTGGCTCCCTCCCTCATAAAAGACTCGACTTTGTGGTAGTATTGGACAACATCTCGTCAAGCAGTGGCTATTTTTCACGCTGGTCTAGGCGAATTGGGAATGTCGGAGGTAGTAGTAGTTACGACCCAGCTGAGAGTGGAAGCGATAGGAAGAGACTTAAGCGTTCATTTCGATCCAAAACAATAAAGGTGCTGATAAATTATGCTGCTAAAATCCCAATGCAAGCGATCGTAAATGGATCACGAGGTCAGGCTTCAAAGGAGTTTCAGGAAGCAGTTAGAGTTCTGGACATCATTCTAAGGCAGCATGCAGTAAAGCAGGGTTGTCTCCTTGTCCGACAGTCTTTCTTCCGTAACAATCCAAGGAACCCAGACTTGGGAGAAGGCTTGTCAGGCTGCTCGGGCTTCCATTCAAGTTTCCGAGCAACCCAAGCGGGTCTGTCCCTGAACATGGATGTATCCACTACAGTGATCATAAAACCAGGTCCCGTTTTGAACTTCCTCTTGGAGAACCAAAAAGTTAATCACCTTCGCCATATTGATTGGATCAAGGTACTTAAAAGTGATTTCATACAGTATTGGCATTGCATTGATATGACTTCATTCATGAGTCCTAGGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

398

Amino Acids

44.49

Weight (kDa)

9.62

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 144, 168
AccB7I CCANNNNNTGG 1 cut(s) 341
AciI CCGC 3 cut(s) 168, 234, 1004
AclWI GGATC 6 cut(s) 197, 352, 528, 738, 817, 1124
AcsI RAATTY 4 cut(s) 38, 107, 399, 536
AfaI GTAC 2 cut(s) 123, 1125
AfiI CCNNNNNNNGG 6 cut(s) 265, 330, 341, 946, 947, 1021
AflII CTTAAG 1 cut(s) 728
AflIII ACRYGT 1 cut(s) 435
AgsI TTSAA 8 cut(s) 44, 107, 287, 385, 494, 828, 984, 1066
AjnI CCWGG 3 cut(s) 6, 334, 1051
AjuI GAANNNNNNNTTGG 6 cut(s) 61, 93, 929, 961, 1058, 1090
AleI CACNNNNGTG 1 cut(s) 1037
AluBI AGCT 3 cut(s) 215, 220, 701
AluI AGCT 3 cut(s) 215, 220, 701
Alw26I GTCTC 3 cut(s) 500, 718, 902
AlwI GGATC 6 cut(s) 197, 352, 528, 738, 817, 1124
Ama87I CYCGRG 1 cut(s) 970
AoxI GGCC 2 cut(s) 64, 332
ApeKI GCWGC 3 cut(s) 776, 874, 966
ApoI RAATTY 4 cut(s) 38, 107, 399, 536
Asp700I GAANNNNTTC 1 cut(s) 563
AspA2I CCTAGG 1 cut(s) 1184
AspLEI GCGC 1 cut(s) 483
AspS9I GGNCC 2 cut(s) 65, 1055
AsuHPI GGTGA 1 cut(s) 1088
AvaI CYCGRG 1 cut(s) 970
AvaII GGWCC 1 cut(s) 1055
AvrII CCTAGG 1 cut(s) 1184
BauI CACGAG 1 cut(s) 813
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 3 cut(s) 763, 886, 953
BccI CCATC 4 cut(s) 310, 401, 443, 542
BciT130I CCWGG 3 cut(s) 8, 336, 1053
BciVI GTATCC 1 cut(s) 1039
BclI TGATCA 1 cut(s) 1041
BcoDI GTCTC 3 cut(s) 500, 718, 902
BfaI CTAG 4 cut(s) 348, 524, 657, 1185
BfmI CTRYAG 2 cut(s) 137, 1035
BfrI CTTAAG 1 cut(s) 728
BfuAI ACCTGC 2 cut(s) 144, 168
BfuI GTATCC 1 cut(s) 1039
BisI GCNGC 3 cut(s) 777, 875, 967
BlnI CCTAGG 1 cut(s) 1184
BlpI GCTNAGC 1 cut(s) 216
BlsI GCNGC 3 cut(s) 778, 876, 968
Bme1390I CCNGG 3 cut(s) 8, 336, 1053
Bme18I GGWCC 1 cut(s) 1055
BmeT110I CYCGRG 1 cut(s) 970
BmgT120I GGNCC 2 cut(s) 65, 1055
BmiI GGNNCC 4 cut(s) 454, 577, 938, 1057
BmrFI CCNGG 3 cut(s) 8, 336, 1053
BmrI ACTGGG 1 cut(s) 452
BmsI GCATC 1 cut(s) 369
BmuI ACTGGG 1 cut(s) 452
BpiI GAAGAC 1 cut(s) 189
Bpu1102I GCTNAGC 1 cut(s) 216
BsaJI CCNNGG 3 cut(s) 61, 932, 1184
Bsc4I CCNNNNNNNGG 6 cut(s) 265, 330, 341, 946, 947, 1021
Bse1I ACTGG 1 cut(s) 458
Bse3DI GCAATG 1 cut(s) 1153
BseBI CCWGG 3 cut(s) 8, 336, 1053
BseDI CCNNGG 3 cut(s) 61, 932, 1184
BseGI GGATG 3 cut(s) 360, 454, 1030
BseLI CCNNNNNNNGG 6 cut(s) 265, 330, 341, 946, 947, 1021
BseMI GCAATG 1 cut(s) 1153
BseMII CTCAG 2 cut(s) 207, 693
BseNI ACTGG 1 cut(s) 458
BseRI GAGGAG 2 cut(s) 216, 219
BseXI GCAGC 3 cut(s) 763, 886, 953
BseYI CCCAGC 1 cut(s) 697
Bsh1285I CGRYCG 1 cut(s) 801
BshFI GGCC 2 cut(s) 66, 334
BsiEI CGRYCG 1 cut(s) 801
BsiHKCI CYCGRG 1 cut(s) 970
BslFI GGGAC 3 cut(s) 242, 997, 1041
BslI CCNNNNNNNGG 6 cut(s) 265, 330, 341, 946, 947, 1021
BsmAI GTCTC 3 cut(s) 500, 718, 902
BsmFI GGGAC 3 cut(s) 242, 997, 1041
BsnI GGCC 2 cut(s) 66, 334
BsoBI CYCGRG 1 cut(s) 970
Bsp143I GATC 8 cut(s) 202, 344, 520, 743, 798, 809, 1041, 1116
Bsp1720I GCTNAGC 1 cut(s) 216
BspACI CCGC 3 cut(s) 168, 234, 1004
BspANI GGCC 2 cut(s) 66, 334
BspCNI CTCAG 2 cut(s) 208, 694
BspHI TCATGA 1 cut(s) 1176
BspLI GGNNCC 4 cut(s) 454, 577, 938, 1057
BspMAI CTGCAG 1 cut(s) 141
BspMI ACCTGC 2 cut(s) 144, 168
BspPI GGATC 6 cut(s) 197, 352, 528, 738, 817, 1124
BspTI CTTAAG 1 cut(s) 728
BsrDI GCAATG 1 cut(s) 1153
BsrI ACTGG 1 cut(s) 458
BssECI CCNNGG 3 cut(s) 61, 932, 1184
BssMI GATC 8 cut(s) 202, 344, 520, 743, 798, 809, 1041, 1116
BssSI CACGAG 1 cut(s) 813
BssT1I CCWWGG 2 cut(s) 932, 1184
Bst2BI CACGAG 1 cut(s) 813
Bst2UI CCWGG 3 cut(s) 8, 336, 1053
Bst4CI ACNGT 4 cut(s) 571, 912, 1039, 1146
Bst6I CTCTTC 1 cut(s) 716
BstAFI CTTAAG 1 cut(s) 728
BstC8I GCNNGC 2 cut(s) 795, 879
BstDEI CTNAG 3 cut(s) 216, 702, 869
BstF5I GGATG 3 cut(s) 360, 454, 1030
BstHHI GCGC 1 cut(s) 483
BstKTI GATC 8 cut(s) 205, 347, 523, 746, 801, 812, 1044, 1119
BstMAI GTCTC 3 cut(s) 500, 718, 902
BstMBI GATC 8 cut(s) 202, 344, 520, 743, 798, 809, 1041, 1116
BstMCI CGRYCG 1 cut(s) 801
BstMWI GCNNNNNNNGC 4 cut(s) 174, 963, 972, 1001
BstNI CCWGG 3 cut(s) 8, 336, 1053
BstNSI RCATGY 2 cut(s) 439, 881
BstSCI CCNGG 3 cut(s) 6, 334, 1051
BstSFI CTRYAG 2 cut(s) 137, 1035
BstV1I GCAGC 3 cut(s) 763, 886, 953
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 2 cut(s) 344, 520
BstYI RGATCY 2 cut(s) 344, 520
BsuI GTATCC 1 cut(s) 1039
BsuRI GGCC 2 cut(s) 66, 334
BtsCI GGATG 3 cut(s) 360, 454, 1030
BtsI GCAGTG 1 cut(s) 640
BtsIMutI CAGTG 3 cut(s) 567, 640, 1044
BveI ACCTGC 2 cut(s) 144, 168
Cac8I GCNNGC 2 cut(s) 795, 879
CciI TCATGA 1 cut(s) 1176
CfoI GCGC 1 cut(s) 483
Cfr13I GGNCC 2 cut(s) 65, 1055
CsiI ACCWGGT 1 cut(s) 1051
Csp6I GTAC 2 cut(s) 122, 1124
CviAII CATG 6 cut(s) 82, 325, 436, 878, 1021, 1177
CviQI GTAC 2 cut(s) 122, 1124
DdeI CTNAG 3 cut(s) 216, 702, 869
DpnI GATC 8 cut(s) 204, 346, 522, 745, 800, 811, 1043, 1118
DpnII GATC 8 cut(s) 202, 344, 520, 743, 798, 809, 1041, 1116
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
Eco130I CCWWGG 2 cut(s) 932, 1184
Eco47I GGWCC 1 cut(s) 1055
Eco88I CYCGRG 1 cut(s) 970
EcoO109I RGGNCCY 1 cut(s) 1055
EcoRII CCWGG 3 cut(s) 6, 334, 1051
EcoT14I CCWWGG 2 cut(s) 932, 1184
ErhI CCWWGG 2 cut(s) 932, 1184
FaeI CATG 6 cut(s) 85, 328, 439, 881, 1024, 1180
FaqI GGGAC 3 cut(s) 242, 997, 1041
FatI CATG 6 cut(s) 81, 324, 435, 877, 1020, 1176
FauI CCCGC 3 cut(s) 161, 241, 997
FauNDI CATATG 1 cut(s) 511
FbaI TGATCA 1 cut(s) 1041
Fnu4HI GCNGC 3 cut(s) 777, 875, 967
FokI GGATG 3 cut(s) 347, 461, 1037
Fsp4HI GCNGC 3 cut(s) 777, 875, 967
FspBI CTAG 4 cut(s) 348, 524, 657, 1185
GlaI GCGC 1 cut(s) 482
GluI GCNGC 3 cut(s) 777, 875, 967
GsaI CCCAGC 1 cut(s) 701
HaeIII GGCC 2 cut(s) 66, 334
HhaI GCGC 1 cut(s) 483
Hin1II CATG 6 cut(s) 85, 328, 439, 881, 1024, 1180
Hin6I GCGC 1 cut(s) 481
HinP1I GCGC 1 cut(s) 481
HinfI GANTC 3 cut(s) 194, 594, 1180
HphI GGTGA 1 cut(s) 1088
Hpy166II GTNNAC 2 cut(s) 144, 567
Hpy188I TCNGA 6 cut(s) 202, 406, 677, 908, 992, 1193
Hpy188III TCNNGA 6 cut(s) 95, 348, 813, 839, 857, 1177
Hpy8I GTNNAC 2 cut(s) 144, 567
HpyAV CCTTC 3 cut(s) 266, 947, 1109
HpyCH4III ACNGT 4 cut(s) 571, 912, 1039, 1146
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 4 cut(s) 139, 793, 881, 1158
HpyF10VI GCNNNNNNNGC 4 cut(s) 174, 963, 972, 1001
HpyF3I CTNAG 3 cut(s) 216, 702, 869
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 6 cut(s) 85, 328, 439, 881, 1024, 1180
HspAI GCGC 1 cut(s) 481
Ksp22I TGATCA 1 cut(s) 1041
Kzo9I GATC 8 cut(s) 202, 344, 520, 743, 798, 809, 1041, 1116
LmnI GCTCC 2 cut(s) 212, 581
Lsp1109I GCAGC 3 cut(s) 763, 886, 953
LweI GCATC 1 cut(s) 369
MabI ACCWGGT 1 cut(s) 1051
MaeI CTAG 4 cut(s) 348, 524, 657, 1185
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 4 cut(s) 131, 460, 689, 923
MalI GATC 8 cut(s) 204, 346, 522, 745, 800, 811, 1043, 1118
MboI GATC 8 cut(s) 202, 344, 520, 743, 798, 809, 1041, 1116
MboII GAAGA 4 cut(s) 189, 568, 733, 910
MfeI CAATTG 1 cut(s) 163
MflI RGATCY 2 cut(s) 344, 520
MluCI AATT 7 cut(s) 38, 107, 163, 399, 536, 663, 769
MlyI GAGTC 3 cut(s) 203, 588, 1189
MmeI TCCRAC 3 cut(s) 388, 655, 931
MroXI GAANNNNTTC 1 cut(s) 563
MseI TTAA 4 cut(s) 501, 729, 1092, 1128
MslI CAYNNNNRTG 2 cut(s) 1037, 1163
MspA1I CMGCKG 1 cut(s) 701
MspCI CTTAAG 1 cut(s) 728
MspR9I CCNGG 3 cut(s) 8, 336, 1053
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 3 cut(s) 8, 336, 1053
MwoI GCNNNNNNNGC 4 cut(s) 174, 963, 972, 1001
NdeI CATATG 1 cut(s) 511
NdeII GATC 8 cut(s) 202, 344, 520, 743, 798, 809, 1041, 1116
NlaIII CATG 6 cut(s) 85, 328, 439, 881, 1024, 1180
NlaIV GGNNCC 4 cut(s) 454, 577, 938, 1057
NspI RCATGY 2 cut(s) 439, 881
OliI CACNNNNGTG 1 cut(s) 1037
PaeI GCATGC 1 cut(s) 881
PagI TCATGA 1 cut(s) 1176
PciI ACATGT 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 341
PkrI GCNGC 3 cut(s) 778, 876, 968
Ple19I CGATCG 1 cut(s) 801
PleI GAGTC 3 cut(s) 202, 588, 1188
PpsI GAGTC 3 cut(s) 202, 588, 1188
PpuMI RGGWCCY 1 cut(s) 1055
PscI ACATGT 1 cut(s) 435
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 3 cut(s) 6, 334, 1051
PspFI CCCAGC 1 cut(s) 697
PspGI CCWGG 3 cut(s) 6, 334, 1051
PspN4I GGNNCC 4 cut(s) 454, 577, 938, 1057
PspPI GGNCC 2 cut(s) 65, 1055
PspPPI RGGWCCY 1 cut(s) 1055
PsrI GAACNNNNNNTAC 2 cut(s) 445, 477
PstI CTGCAG 1 cut(s) 141
PsuI RGATCY 2 cut(s) 344, 520
PvuI CGATCG 1 cut(s) 801
PvuII CAGCTG 1 cut(s) 701
RsaI GTAC 2 cut(s) 123, 1125
RsaNI GTAC 2 cut(s) 122, 1124
RseI CAYNNNNRTG 2 cut(s) 1037, 1163
SaqAI TTAA 4 cut(s) 501, 729, 1092, 1128
SatI GCNGC 3 cut(s) 777, 875, 967
Sau3AI GATC 8 cut(s) 202, 344, 520, 743, 798, 809, 1041, 1116
Sau96I GGNCC 2 cut(s) 65, 1055
SchI GAGTC 3 cut(s) 203, 588, 1189
ScrFI CCNGG 3 cut(s) 8, 336, 1053
SexAI ACCWGGT 1 cut(s) 1051
SfaNI GCATC 1 cut(s) 369
SfcI CTRYAG 2 cut(s) 137, 1035
SinI GGWCC 1 cut(s) 1055
SmiMI CAYNNNNRTG 2 cut(s) 1037, 1163
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SphI GCATGC 1 cut(s) 881
Sse9I AATT 7 cut(s) 38, 107, 163, 399, 536, 663, 769
SsiI CCGC 3 cut(s) 168, 234, 1004
SspMI CTAG 4 cut(s) 348, 524, 657, 1185
StyD4I CCNGG 3 cut(s) 6, 334, 1051
StyI CCWWGG 2 cut(s) 932, 1184
TaaI ACNGT 4 cut(s) 571, 912, 1039, 1146
TaiI ACGT 1 cut(s) 75
TaqI TCGA 2 cut(s) 597, 742
TasI AATT 7 cut(s) 38, 107, 163, 399, 536, 663, 769
Tru1I TTAA 4 cut(s) 501, 729, 1092, 1128
Tru9I TTAA 4 cut(s) 501, 729, 1092, 1128
TscAI CASTG 3 cut(s) 574, 640, 1044
TseI GCWGC 3 cut(s) 776, 874, 966
TspDTI ATGAA 4 cut(s) 726, 1129, 1161, 1165
TspGWI ACGGA 2 cut(s) 63, 911
TspRI CASTG 3 cut(s) 574, 640, 1044
Van91I CCANNNNNTGG 1 cut(s) 341
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 1055
XapI RAATTY 4 cut(s) 38, 107, 399, 536
XbaI TCTAGA 1 cut(s) 347
XceI RCATGY 2 cut(s) 439, 881
XmaJI CCTAGG 1 cut(s) 1184
XmnI GAANNNNTTC 1 cut(s) 563
XspI CTAG 4 cut(s) 348, 524, 657, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.