Rh1DG112200

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001623
Physical Location & Seq
Forward (+)
20717112 .. 20724249
7138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG112200.1

Sequence Viewer

Length: 468 bp
ATGAATGGAAGACAAGAAACACTGGATTTAATAATGAGCAGAGGTCACAGTCGTATACCAATCTATTCAGGAAATCTAAATAATATCATTGGCCTCATTTTGTTTTCTCTTTTCAGGGATGGAGTGAGTGAATCACAGTTCAACCAAGTCTTGAATGTAGAACTGGATCAGATTATTGAGGCCTGCAAGTTTCTTGACGAGAGCTGGTGTCCAAAGTCCATGGTTATTATTGCACAAAAGAATCATCATACAAAATTTTTTCAGGCTGGTGGTTCTCCTGACAATGTTCCACCTGGGACTATTATTGACAACAAAGTCTGTCATCCAAAGAACAATGATTTCTACTTATGTTCTCATGCTGGGATTATTGGAACAACTCGTCCTACTCATTATCATGTTCTATATGATGAACATGGCTTCTCAGCGGATGACTTGCAAGAACTTGTTCATTCTTTATCATATGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

155

Amino Acids

17.43

Weight (kDa)

5.77

Isoelectric Point (pI)

47.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Piwi PF02171 37 - 154 2e-48 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 55
AciI CCGC 1 cut(s) 425
AclWI GGATC 1 cut(s) 174
AcsI RAATTY 1 cut(s) 254
AgsI TTSAA 2 cut(s) 142, 154
AjnI CCWGG 1 cut(s) 292
AloI GAACNNNNNNTCC 2 cut(s) 364, 396
AluBI AGCT 1 cut(s) 204
AluI AGCT 1 cut(s) 204
AlwI GGATC 1 cut(s) 174
AoxI GGCC 2 cut(s) 91, 180
ApoI RAATTY 1 cut(s) 254
Asp700I GAANNNNTTC 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 16
BccI CCATC 1 cut(s) 113
BciT130I CCWGG 1 cut(s) 294
Bme1390I CCNGG 1 cut(s) 294
BmrFI CCNGG 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 16
BsaJI CCNNGG 2 cut(s) 219, 293
Bse1I ACTGG 2 cut(s) 27, 168
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 219, 293
BseGI GGATG 3 cut(s) 124, 322, 433
BseMII CTCAG 1 cut(s) 435
BseNI ACTGG 2 cut(s) 27, 168
BseYI CCCAGC 1 cut(s) 359
BshFI GGCC 2 cut(s) 93, 182
BslFI GGGAC 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 310
BsnI GGCC 2 cut(s) 93, 182
Bsp143I GATC 1 cut(s) 166
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 1 cut(s) 425
BspANI GGCC 2 cut(s) 93, 182
BspCNI CTCAG 1 cut(s) 434
BspPI GGATC 1 cut(s) 174
BsrI ACTGG 2 cut(s) 27, 168
BssECI CCNNGG 2 cut(s) 219, 293
BssMI GATC 1 cut(s) 166
BssNAI GTATAC 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 50, 138
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 1 cut(s) 421
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 3 cut(s) 124, 322, 433
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstNI CCWGG 1 cut(s) 294
BstSCI CCNGG 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 16
BstZ17I GTATAC 1 cut(s) 56
BsuRI GGCC 2 cut(s) 93, 182
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 3 cut(s) 124, 322, 433
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 184
CviAII CATG 4 cut(s) 220, 356, 395, 413
CviJI RGCY 5 cut(s) 93, 182, 204, 266, 417
CviKI_1 RGCY 5 cut(s) 93, 182, 204, 266, 417
DdeI CTNAG 1 cut(s) 421
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
DrdI GACNNNNNNGTC 1 cut(s) 314
DseDI GACNNNNNNGTC 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 219
Eco147I AGGCCT 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 4 cut(s) 223, 359, 398, 416
FaqI GGGAC 1 cut(s) 310
FatI CATG 4 cut(s) 219, 355, 394, 412
FauNDI CATATG 1 cut(s) 460
FblI GTMKAC 1 cut(s) 55
FokI GGATG 3 cut(s) 131, 309, 440
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 2 cut(s) 93, 182
Hin1II CATG 4 cut(s) 223, 359, 398, 416
HinfI GANTC 2 cut(s) 131, 241
Hpy166II GTNNAC 1 cut(s) 56
Hpy188I TCNGA 1 cut(s) 171
Hpy188III TCNNGA 4 cut(s) 69, 151, 194, 278
Hpy8I GTNNAC 1 cut(s) 56
HpyCH4III ACNGT 2 cut(s) 50, 138
HpyCH4V TGCA 3 cut(s) 186, 233, 436
HpyF3I CTNAG 1 cut(s) 421
Hsp92II CATG 4 cut(s) 223, 359, 398, 416
Kzo9I GATC 1 cut(s) 166
MaeIII GTNAC 1 cut(s) 44
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 1 cut(s) 21
MluCI AATT 1 cut(s) 254
MnlI CCTC 3 cut(s) 35, 104, 172
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 1 cut(s) 29
MslI CAYNNNNRTG 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 460
NdeII GATC 1 cut(s) 166
NlaIII CATG 4 cut(s) 223, 359, 398, 416
NmuCI GTSAC 1 cut(s) 44
PceI AGGCCT 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 2 cut(s) 131, 241
Psp6I CCWGG 1 cut(s) 292
PspFI CCCAGC 1 cut(s) 359
PspGI CCWGG 1 cut(s) 292
RseI CAYNNNNRTG 1 cut(s) 393
SaqAI TTAA 1 cut(s) 29
Sau3AI GATC 1 cut(s) 166
ScrFI CCNGG 1 cut(s) 294
SetI ASST 3 cut(s) 46, 206, 295
SmiMI CAYNNNNRTG 1 cut(s) 393
Sse9I AATT 1 cut(s) 254
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 1 cut(s) 425
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 2 cut(s) 50, 138
TasI AATT 1 cut(s) 254
TfiI GAWTC 2 cut(s) 131, 241
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 1 cut(s) 27
TseFI GTSAC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 3 cut(s) 17, 423, 437
TspRI CASTG 1 cut(s) 27
XapI RAATTY 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 55
XmnI GAANNNNTTC 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.