RLG00000008257

gene silencing by RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001623
Physical Location & Seq
Forward (+)
27862424 .. 27865102
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008257

Sequence Viewer

Length: 2586 bp
ATGTCGACGAAGGCGTTAATTGTAGCAGGAGAAAAACCTCTTGAACAAATTAACAATGCATCTACTCGAATACGTGTTCCCATGAAGAGGTCTGGCATTGGATCTAGAGGTAAAAGGATTCCACTTCTTACCAACCATTTCAAGGTGGGAGTGAACAAATATACAGATGACTTTTACCACTATAGTATTGCAATGCAATATGATGACGGAACTCCAGTTGATGGAAAAGGCATTGGATGCAAAGTTCTTGATAAAATTAAAGAGATATACGGCATGGAGCTGGCAAACAAAGAACTTGCTTATGATGGAGAGAAGAGCTTGTTTACTGTTGGCCAACTCCCTCATAAAAGACTTGACTTCATGATCGTATTGGATGACATGTCATTGAGCAGGTCTGGGGGATCCAGACGCGGTTACTTTTTAGAAGAGAGGAAGGGTGATAGGAAGAGAGTAAAGCAGTCGTTTGGTTCCAAAACAATAAAGGTGCAGTTCAACTTTGCTGCTATAATCCCAATGCAAGCAATTGCGAATGCCTTACGAGGTCAGGACTCTGACCATTTTCAGGAAGCAGTTAGAGTTCTTGACATCATTCTAAGGCAGAATGCAGCAAAGCAGGGTTGTCTCCTTGTTCGGCAGTCATTCTTCCATAACAAGCCGAGGAGCTTTATAGAGCTGGGAGGAGGGGTGTTAGGCTGTAGGGGTTTACATTCAAGCTTCCGGGCCACCCAAGGTGGCCTATGTCTGAATATGGATGTATCCACTACAATGATTCTAAAACCTGGTCCTGTTGTGAATTTCCTCATCGAGAACCAAAATGTCAAAACTCCTTACCAGATTGATTGGACTAAGGCTAAAAGGATGCTTAAAAATCTGAAGATTGTGACCTATGCCTCCAAGAAGGAGTACAAAATCACTGGATTGAGTGATAAACCCTGCAGACAGCAGACCTTCTTTTTGAATTCAAAAAAAGGACAGAATGGAGATGGAGTAGAGATCACAGTGTGTGAATACTTTGCTCAGTATAAGCAGATGCAGTTACGCGATTCAGCAGATTTTCCATGCATTAATATTGGAAAGCCAAAGAAACCATCCTACTTTCCTCTTGAGCTTTGTGACTTGGTTTCTTTGCAACGGTATACCAAGTCCTTGTCCAATATCCAAAGGGCTTCACTAGTGGAGAAGTCTAGGCAGAAGCCCCAGGAGCGAATGTCAACTTTGCGTGATGCACTGAGGAGTAGCAAGTATGAGGCTGACGTGATGCTTCGTTCTGCTGGAATATCGATTGGTACAGAACTCATGCAGGTTGAAGGTCGTGTGTTGTCAGCCCCAAATTTGAAAGTGGGAAACGGAGATGTCATTTTTCCTAGGAATGGGAGATGGAATTTCAACAATAAAAAACTAGTAGATCCTGTAAAACTAGAACGCTGGGCTGTTGTGAATTTCTCTGCTCGCTGTGACATTCGGTACCTGGTCGACACTATGATAAAGTGTGGAGATATGAAAGGAATTTCCATCAAGCCTCCATTTGGTGTGTTTGAAGAGAGTATTCAGAACAGACAGAGGAAGAATTCTGAGATATATGGTCCATGGAAAAGGAAAAACCTTTCAGACATTGGGATTGCAACGCAGTGCATTGCACCTACGAAGGTGAACGATCAATACATCACTAACGTGCTCCTGAAAATTAATGCAAAGCTAGGTGGAATGAACTCATTGCTAACTGTGGAGCATTCTCCTTCTCTACCCTTGGTTTCAAAGTCCCCTACTCTGATATTAGGGATGGATGTGTCACATGGCTCACCCGGGCAGTCAAATATGCCTTCTATTGCAGCAGTGGTCAGTTCTAGGCACTGGCCTTGGATTTCTCGTTACCGAGCTGCAGTTCGTACTCAGTCACCAAAAGTAGAAATGATTGCCAATCTGTTCAAGCCTGTGTCTGACAAAGAGGACCAAGGCATAGTCAAGGAACTTTTGCTAGACTTTTATAATACTTCAAACTACAGGAAGCCGGAGCAGATTATTATCTTCAGGGATGGAGTGAGTGAATCACAGTTCAACCAAGTCTTGAATCTGGAATTGGATCAGATTATTAAGGCCTGCAAGTTCATTGAGGAGAGCTGGTCGCCAAAGTTCATGGTTATTATTGCACAAAAGAGACATCACACAAAATTCTTTCAGGCTGGTAGTTCTTCTGATAATGTTCCACCTGGGACTATCGTTGACAACAAAGTCTGTGATGCAAGAAACAATGATTTCTACTTGTGTGCTCATGCTGGGATGATAGGAACAAGTCGGCCTACTCATTATCATGTTCTATATGATGAACTTGGCTTTTCAGCAGATGACTTGCAAGAACTAGTTCATTCTTTGTCGTATGTTTATCAAAGGAGCACGACGGCCGTATCTCTAGTTGCTCCAATATGCTACGCCCATCTAGCAGCAGCACAGATGAGTCAGTTCATTAGGTTTGATGAAATGTCAGAGACTGCCTCAAGCCAAGGTAGTGTTACTGTTGCTGGAGATGTGCCGGTGCAGGAATTACCCAGGCTACATAAAAATGTCGTCAACTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

862

Amino Acids

96.75

Weight (kDa)

9.44

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 38 - 198 3.4e-23 N-terminal domain of argonaute
ArgoL1 PF08699 210 - 258 4.1e-16 Argonaute linker 1 domain
PAZ PF02170 265 - 391 1.9e-25 PAZ domain
ArgoL2 PF16488 401 - 446 1.6e-09 Argonaute linker 2 domain
Piwi PF02171 521 - 821 1e-102 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1986
AasI GACNNNNNNGTC 1 cut(s) 2228
Acc36I ACCTGC 2 cut(s) 381, 1291
Acc65I GGTACC 1 cut(s) 1464
AccB1I GGYRCC 1 cut(s) 1464
AccB7I CCANNNNNTGG 1 cut(s) 221
AccI GTMKAC 3 cut(s) 5, 1136, 1473
AccII CGCG 2 cut(s) 411, 1041
AciI CCGC 1 cut(s) 411
AclWI GGATC 5 cut(s) 109, 396, 409, 1400, 2088
AcoI YGGCCR 2 cut(s) 331, 2397
AcsI RAATTY 8 cut(s) 793, 958, 1330, 1381, 1438, 1506, 1567, 2168
AcuI CTGAAG 2 cut(s) 893, 2011
AdeI CACNNNGTG 1 cut(s) 1002
AfaI GTAC 4 cut(s) 905, 1288, 1466, 1888
AfiI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1370, 1526
AflIII ACRYGT 2 cut(s) 73, 378
AhlI ACTAGT 3 cut(s) 1171, 1399, 2356
AjiI CACGTC 1 cut(s) 1255
AjnI CCWGG 5 cut(s) 778, 1197, 1467, 2206, 2543
AjuI GAANNNNNNNTTGG 2 cut(s) 2060, 2092
AleI CACNNNNGTG 1 cut(s) 1670
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
AluBI AGCT 9 cut(s) 280, 318, 663, 673, 714, 1108, 1696, 1877, 2118
AluI AGCT 9 cut(s) 280, 318, 663, 673, 714, 1108, 1696, 1877, 2118
Alw21I GWGCWC 3 cut(s) 1677, 2269, 2393
Alw26I GTCTC 3 cut(s) 626, 2149, 2477
AlwI GGATC 5 cut(s) 109, 396, 409, 1400, 2088
AlwNI CAGNNNCTG 1 cut(s) 2486
Ama87I CYCGRG 1 cut(s) 1802
AoxI GGCC 7 cut(s) 331, 720, 733, 1853, 2094, 2294, 2397
ApeKI GCWGC 6 cut(s) 500, 605, 1829, 1877, 2438, 2441
ApoI RAATTY 8 cut(s) 793, 958, 1330, 1381, 1438, 1506, 1567, 2168
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AseI ATTAAT 2 cut(s) 1065, 1686
Asp700I GAANNNNTTC 1 cut(s) 2358
Asp718I GGTACC 1 cut(s) 1464
AspA2I CCTAGG 1 cut(s) 1364
AspS9I GGNCC 4 cut(s) 720, 782, 1583, 1948
AsuC2I CCSGG 3 cut(s) 719, 1803, 1804
AsuHPI GGTGA 4 cut(s) 449, 1660, 1791, 1887
AvaI CYCGRG 1 cut(s) 1802
AvaII GGWCC 3 cut(s) 782, 1583, 1948
AvrII CCTAGG 1 cut(s) 1364
BaeI ACNNNNGTAYC 2 cut(s) 2385, 2418
BalI TGGCCA 1 cut(s) 333
BamHI GGATCC 1 cut(s) 401
BanI GGYRCC 1 cut(s) 1464
Bbv12I GWGCWC 3 cut(s) 1677, 2269, 2393
BbvI GCAGC 6 cut(s) 487, 617, 1841, 1864, 2450, 2453
BccI CCATC 9 cut(s) 215, 299, 977, 1096, 1371, 1520, 1774, 2027, 2439
BceAI ACGGC 3 cut(s) 286, 2384, 2412
BciT130I CCWGG 5 cut(s) 780, 1199, 1469, 2208, 2545
BciVI GTATCC 1 cut(s) 766
BcnI CCSGG 3 cut(s) 719, 1803, 1804
BcoDI GTCTC 3 cut(s) 626, 2149, 2477
BcuI ACTAGT 3 cut(s) 1171, 1399, 2356
BfmI CTRYAG 5 cut(s) 181, 694, 934, 1878, 1999
BfuAI ACCTGC 2 cut(s) 381, 1291
BfuI GTATCC 1 cut(s) 766
BisI GCNGC 6 cut(s) 501, 606, 1830, 1878, 2439, 2442
BlnI CCTAGG 1 cut(s) 1364
BlsI GCNGC 6 cut(s) 502, 607, 1831, 1879, 2440, 2443
Bme1390I CCNGG 8 cut(s) 719, 780, 1199, 1469, 1803, 1804, 2208, 2545
Bme18I GGWCC 3 cut(s) 782, 1583, 1948
BmeT110I CYCGRG 1 cut(s) 1802
BmgBI CACGTC 1 cut(s) 1255
BmgT120I GGNCC 4 cut(s) 720, 782, 1583, 1948
BmiI GGNNCC 3 cut(s) 403, 469, 1466
BmrFI CCNGG 8 cut(s) 719, 780, 1199, 1469, 1803, 1804, 2208, 2545
BmsI GCATC 7 cut(s) 68, 227, 849, 1020, 1213, 1248, 2227
BplI GAGNNNNNCTC 2 cut(s) 2474, 2506
BpmI CTGGAG 2 cut(s) 198, 2538
BpuEI CTTGAG 2 cut(s) 1124, 2476
BpuMI CCSGG 3 cut(s) 719, 1803, 1804
Bsa29I ATCGAT 1 cut(s) 1280
BsaAI YACGTR 1 cut(s) 74
BsaBI GATNNNNATC 1 cut(s) 2021
BsaXI ACNNNNNCTCC 8 cut(s) 669, 699, 972, 1002, 1193, 1223, 2105, 2135
Bsc4I CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1370, 1526
Bse118I RCCGGY 1 cut(s) 2527
Bse1I ACTGG 3 cut(s) 215, 919, 1856
Bse3DI GCAATG 3 cut(s) 198, 1632, 1712
Bse8I GATNNNNATC 1 cut(s) 2021
BseBI CCWGG 5 cut(s) 780, 1199, 1469, 2208, 2545
BseCI ATCGAT 1 cut(s) 1280
BseGI GGATG 9 cut(s) 242, 379, 757, 864, 1088, 1785, 1789, 2038, 2283
BseJI GATNNNNATC 1 cut(s) 2021
BseLI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1370, 1526
BseMI GCAATG 3 cut(s) 198, 1632, 1712
BseMII CTCAG 4 cut(s) 1031, 1220, 1563, 1904
BseNI ACTGG 3 cut(s) 215, 919, 1856
BseRI GAGGAG 4 cut(s) 673, 693, 1246, 2126
BseX3I CGGCCG 1 cut(s) 2397
BseXI GCAGC 6 cut(s) 487, 617, 1841, 1864, 2450, 2453
BseYI CCCAGC 3 cut(s) 673, 1425, 2273
BsgI GTGCAG 2 cut(s) 506, 2552
Bsh1236I CGCG 2 cut(s) 411, 1041
Bsh1285I CGRYCG 1 cut(s) 2400
BshFI GGCC 7 cut(s) 333, 722, 735, 1855, 2096, 2296, 2399
BshNI GGYRCC 1 cut(s) 1464
BshVI ATCGAT 1 cut(s) 1280
BsiEI CGRYCG 1 cut(s) 2400
BsiHKAI GWGCWC 3 cut(s) 1677, 2269, 2393
BsiHKCI CYCGRG 1 cut(s) 1802
BsiSI CCGG 4 cut(s) 718, 1803, 2009, 2528
BslFI GGGAC 2 cut(s) 1744, 2224
BslI CCNNNNNNNGG 6 cut(s) 87, 142, 221, 562, 1370, 1526
BsmAI GTCTC 3 cut(s) 626, 2149, 2477
BsmFI GGGAC 2 cut(s) 1744, 2224
BsmI GAATGC 3 cut(s) 535, 607, 1729
BsnI GGCC 7 cut(s) 333, 722, 735, 1855, 2096, 2296, 2399
BsoBI CYCGRG 1 cut(s) 1802
Bsp1286I GDGCHC 3 cut(s) 1677, 2269, 2393
Bsp143I GATC 7 cut(s) 101, 363, 401, 993, 1405, 1654, 2080
Bsp19I CCATGG 1 cut(s) 1586
BspACI CCGC 1 cut(s) 411
BspANI GGCC 7 cut(s) 333, 722, 735, 1855, 2096, 2296, 2399
BspCNI CTCAG 4 cut(s) 1030, 1221, 1564, 1903
BspDI ATCGAT 1 cut(s) 1280
BspFNI CGCG 2 cut(s) 411, 1041
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 3 cut(s) 403, 469, 1466
BspMAI CTGCAG 2 cut(s) 938, 1882
BspMI ACCTGC 2 cut(s) 381, 1291
BspPI GGATC 5 cut(s) 109, 396, 409, 1400, 2088
BspQI GCTCTTC 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 1464
BsrDI GCAATG 3 cut(s) 198, 1632, 1712
BsrFI RCCGGY 1 cut(s) 2527
BsrI ACTGG 3 cut(s) 215, 919, 1856
BssAI RCCGGY 1 cut(s) 2527
BssMI GATC 7 cut(s) 101, 363, 401, 993, 1405, 1654, 2080
BssNAI GTATAC 1 cut(s) 1137
BssT1I CCWWGG 7 cut(s) 727, 1364, 1586, 1746, 1856, 1951, 2497
Bst1107I GTATAC 1 cut(s) 1137
Bst2UI CCWGG 5 cut(s) 780, 1199, 1469, 2208, 2545
Bst4CI ACNGT 6 cut(s) 328, 1000, 1134, 1723, 2052, 2512
Bst6I CTCTTC 5 cut(s) 80, 308, 420, 440, 1533
BstAPI GCANNNNNTGC 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 4 cut(s) 282, 519, 1450, 2098
BstDEI CTNAG 6 cut(s) 593, 846, 1017, 1229, 1572, 1890
BstDSI CCRYGG 1 cut(s) 1586
BstF5I GGATG 9 cut(s) 242, 379, 757, 864, 1088, 1785, 1789, 2038, 2283
BstFNI CGCG 2 cut(s) 411, 1041
BstKTI GATC 7 cut(s) 104, 366, 404, 996, 1408, 1657, 2083
BstMAI GTCTC 3 cut(s) 626, 2149, 2477
BstMBI GATC 7 cut(s) 101, 363, 401, 993, 1405, 1654, 2080
BstMCI CGRYCG 1 cut(s) 2400
BstMWI GCNNNNNNNGC 3 cut(s) 237, 1201, 2435
BstNI CCWGG 5 cut(s) 780, 1199, 1469, 2208, 2545
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 8 cut(s) 717, 778, 1197, 1467, 1801, 1802, 2206, 2543
BstSFI CTRYAG 5 cut(s) 181, 694, 934, 1878, 1999
BstUI CGCG 2 cut(s) 411, 1041
BstV1I GCAGC 6 cut(s) 487, 617, 1841, 1864, 2450, 2453
BstX2I RGATCY 3 cut(s) 101, 401, 1405
BstYI RGATCY 3 cut(s) 101, 401, 1405
BstZ17I GTATAC 1 cut(s) 1137
BstZI CGGCCG 1 cut(s) 2397
Bsu15I ATCGAT 1 cut(s) 1280
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 7 cut(s) 333, 722, 735, 1855, 2096, 2296, 2399
BsuTUI ATCGAT 1 cut(s) 1280
BtgI CCRYGG 1 cut(s) 1586
BtrI CACGTC 1 cut(s) 1255
BtsCI GGATG 9 cut(s) 242, 379, 757, 864, 1088, 1785, 1789, 2038, 2283
BtsI GCAGTG 2 cut(s) 1634, 1839
BtsIMutI CAGTG 6 cut(s) 912, 1005, 1226, 1634, 1839, 1849
BveI ACCTGC 2 cut(s) 381, 1291
Cac8I GCNNGC 4 cut(s) 282, 519, 1450, 2098
CaiI CAGNNNCTG 1 cut(s) 2486
CciI TCATGA 1 cut(s) 360
Cfr10I RCCGGY 1 cut(s) 2527
Cfr13I GGNCC 4 cut(s) 720, 782, 1583, 1948
Cfr9I CCCGGG 1 cut(s) 1802
ClaI ATCGAT 1 cut(s) 1280
CseI GACGC 1 cut(s) 417
CsiI ACCWGGT 2 cut(s) 778, 1467
Csp6I GTAC 4 cut(s) 904, 1287, 1465, 1887
CviQI GTAC 4 cut(s) 904, 1287, 1465, 1887
DdeI CTNAG 6 cut(s) 593, 846, 1017, 1229, 1572, 1890
DpnI GATC 7 cut(s) 103, 365, 403, 995, 1407, 1656, 2082
DpnII GATC 7 cut(s) 101, 363, 401, 993, 1405, 1654, 2080
DraIII CACNNNGTG 1 cut(s) 1002
DrdI GACNNNNNNGTC 1 cut(s) 2228
DseDI GACNNNNNNGTC 1 cut(s) 2228
EaeI YGGCCR 2 cut(s) 331, 2397
EagI CGGCCG 1 cut(s) 2397
Eam1104I CTCTTC 5 cut(s) 80, 308, 420, 440, 1533
EarI CTCTTC 5 cut(s) 80, 308, 420, 440, 1533
EclXI CGGCCG 1 cut(s) 2397
Eco130I CCWWGG 7 cut(s) 727, 1364, 1586, 1746, 1856, 1951, 2497
Eco147I AGGCCT 1 cut(s) 2096
Eco47I GGWCC 3 cut(s) 782, 1583, 1948
Eco52I CGGCCG 1 cut(s) 2397
Eco57I CTGAAG 2 cut(s) 893, 2011
Eco88I CYCGRG 1 cut(s) 1802
EcoRI GAATTC 2 cut(s) 958, 1567
EcoRII CCWGG 5 cut(s) 778, 1197, 1467, 2206, 2543
EcoT14I CCWWGG 7 cut(s) 727, 1364, 1586, 1746, 1856, 1951, 2497
EcoT22I ATGCAT 2 cut(s) 61, 1064
ErhI CCWWGG 7 cut(s) 727, 1364, 1586, 1746, 1856, 1951, 2497
FaqI GGGAC 2 cut(s) 1744, 2224
FblI GTMKAC 3 cut(s) 5, 1136, 1473
Fnu4HI GCNGC 6 cut(s) 501, 606, 1830, 1878, 2439, 2442
FokI GGATG 9 cut(s) 249, 386, 764, 871, 1075, 1792, 1796, 2045, 2290
Fsp4HI GCNGC 6 cut(s) 501, 606, 1830, 1878, 2439, 2442
GluI GCNGC 6 cut(s) 501, 606, 1830, 1878, 2439, 2442
GsaI CCCAGC 3 cut(s) 677, 1429, 2277
GsuI CTGGAG 2 cut(s) 198, 2538
HaeIII GGCC 7 cut(s) 333, 722, 735, 1855, 2096, 2296, 2399
HapII CCGG 4 cut(s) 718, 1803, 2009, 2528
HgaI GACGC 1 cut(s) 417
HincII GTYRAC 5 cut(s) 6, 1212, 1474, 2221, 2566
HindII GTYRAC 5 cut(s) 6, 1212, 1474, 2221, 2566
HindIII AAGCTT 1 cut(s) 712
HinfI GANTC 7 cut(s) 118, 548, 769, 1043, 2045, 2068, 2452
HpaII CCGG 4 cut(s) 718, 1803, 2009, 2528
HphI GGTGA 4 cut(s) 449, 1660, 1791, 1887
Hpy99I CGWCG 2 cut(s) 10, 2398
HpyAV CCTTC 8 cut(s) 4, 427, 892, 958, 1301, 1639, 1746, 1830
HpyCH4III ACNGT 6 cut(s) 328, 1000, 1134, 1723, 2052, 2512
HpyCH4IV ACGT 3 cut(s) 73, 1254, 1671
HpyF10VI GCNNNNNNNGC 3 cut(s) 237, 1201, 2435
HpyF3I CTNAG 6 cut(s) 593, 846, 1017, 1229, 1572, 1890
HpySE526I ACGT 3 cut(s) 73, 1254, 1671
KpnI GGTACC 1 cut(s) 1468
Kzo9I GATC 7 cut(s) 101, 363, 401, 993, 1405, 1654, 2080
LguI GCTCTTC 1 cut(s) 308
LmnI GCTCC 8 cut(s) 277, 660, 1201, 1680, 1726, 2011, 2388, 2419
Lsp1109I GCAGC 6 cut(s) 487, 617, 1841, 1864, 2450, 2453
LweI GCATC 7 cut(s) 68, 227, 849, 1020, 1213, 1248, 2227
MabI ACCWGGT 2 cut(s) 778, 1467
MaeII ACGT 3 cut(s) 73, 1254, 1671
MaeIII GTNAC 9 cut(s) 413, 880, 1035, 1112, 1454, 1788, 1868, 1893, 2506
MalI GATC 7 cut(s) 103, 365, 403, 995, 1407, 1656, 2082
MboI GATC 7 cut(s) 101, 363, 401, 993, 1405, 1654, 2080
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 3 cut(s) 101, 401, 1405
MhlI GDGCHC 3 cut(s) 1677, 2269, 2393
MlsI TGGCCA 1 cut(s) 333
MluNI TGGCCA 1 cut(s) 333
MlyI GAGTC 2 cut(s) 542, 2461
Mox20I TGGCCA 1 cut(s) 333
Mph1103I ATGCAT 2 cut(s) 61, 1064
MroXI GAANNNNTTC 1 cut(s) 2358
MscI TGGCCA 1 cut(s) 333
MseI TTAA 7 cut(s) 17, 51, 258, 864, 1065, 1686, 2091
MslI CAYNNNNRTG 4 cut(s) 764, 1670, 2307, 2556
Msp20I TGGCCA 1 cut(s) 333
MspI CCGG 4 cut(s) 718, 1803, 2009, 2528
MspR9I CCNGG 8 cut(s) 719, 780, 1199, 1469, 1803, 1804, 2208, 2545
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 3 cut(s) 535, 607, 1729
MvaI CCWGG 5 cut(s) 780, 1199, 1469, 2208, 2545
MvnI CGCG 2 cut(s) 411, 1041
MwoI GCNNNNNNNGC 3 cut(s) 237, 1201, 2435
NciI CCSGG 3 cut(s) 719, 1803, 1804
NcoI CCATGG 1 cut(s) 1586
NdeII GATC 7 cut(s) 101, 363, 401, 993, 1405, 1654, 2080
NlaIV GGNNCC 3 cut(s) 403, 469, 1466
NmeAIII GCCGAG 1 cut(s) 681
NmuCI GTSAC 5 cut(s) 880, 1112, 1454, 1788, 1893
NsiI ATGCAT 2 cut(s) 61, 1064
NspI RCATGY 1 cut(s) 382
OliI CACNNNNGTG 1 cut(s) 1670
PagI TCATGA 1 cut(s) 360
PceI AGGCCT 1 cut(s) 2096
PciI ACATGT 1 cut(s) 378
PciSI GCTCTTC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 11
PctI GAATGC 3 cut(s) 535, 607, 1729
PdmI GAANNNNTTC 1 cut(s) 2358
PfeI GAWTC 5 cut(s) 118, 769, 1043, 2045, 2068
PflMI CCANNNNNTGG 1 cut(s) 221
PkrI GCNGC 6 cut(s) 502, 607, 1831, 1879, 2440, 2443
PleI GAGTC 2 cut(s) 542, 2460
PpsI GAGTC 2 cut(s) 542, 2460
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 1 cut(s) 378
PshBI ATTAAT 2 cut(s) 1065, 1686
PsiI TTATAA 1 cut(s) 1986
Psp6I CCWGG 5 cut(s) 778, 1197, 1467, 2206, 2543
PspFI CCCAGC 3 cut(s) 673, 1425, 2273
PspGI CCWGG 5 cut(s) 778, 1197, 1467, 2206, 2543
PspN4I GGNNCC 3 cut(s) 403, 469, 1466
PspPI GGNCC 4 cut(s) 720, 782, 1583, 1948
PsrI GAACNNNNNNTAC 2 cut(s) 1643, 1675
PstI CTGCAG 2 cut(s) 938, 1882
PstNI CAGNNNCTG 1 cut(s) 2486
PsuI RGATCY 3 cut(s) 101, 401, 1405
RsaI GTAC 4 cut(s) 905, 1288, 1466, 1888
RsaNI GTAC 4 cut(s) 904, 1287, 1465, 1887
RseI CAYNNNNRTG 4 cut(s) 764, 1670, 2307, 2556
SalI GTCGAC 2 cut(s) 4, 1472
SapI GCTCTTC 1 cut(s) 308
SaqAI TTAA 7 cut(s) 17, 51, 258, 864, 1065, 1686, 2091
SatI GCNGC 6 cut(s) 501, 606, 1830, 1878, 2439, 2442
Sau3AI GATC 7 cut(s) 101, 363, 401, 993, 1405, 1654, 2080
Sau96I GGNCC 4 cut(s) 720, 782, 1583, 1948
SchI GAGTC 2 cut(s) 542, 2461
ScrFI CCNGG 8 cut(s) 719, 780, 1199, 1469, 1803, 1804, 2208, 2545
SduI GDGCHC 3 cut(s) 1677, 2269, 2393
SexAI ACCWGGT 2 cut(s) 778, 1467
SfaNI GCATC 7 cut(s) 68, 227, 849, 1020, 1213, 1248, 2227
SfcI CTRYAG 5 cut(s) 181, 694, 934, 1878, 1999
SinI GGWCC 3 cut(s) 782, 1583, 1948
SmaI CCCGGG 1 cut(s) 1804
SmiMI CAYNNNNRTG 4 cut(s) 764, 1670, 2307, 2556
SmlI CTYRAG 2 cut(s) 1103, 2491
SmoI CTYRAG 2 cut(s) 1103, 2491
SpeI ACTAGT 3 cut(s) 1171, 1399, 2356
SseBI AGGCCT 1 cut(s) 2096
SsiI CCGC 1 cut(s) 411
SspI AATATT 1 cut(s) 1069
StuI AGGCCT 1 cut(s) 2096
StyD4I CCNGG 8 cut(s) 717, 778, 1197, 1467, 1801, 1802, 2206, 2543
StyI CCWWGG 7 cut(s) 727, 1364, 1586, 1746, 1856, 1951, 2497
TaaI ACNGT 6 cut(s) 328, 1000, 1134, 1723, 2052, 2512
TaiI ACGT 3 cut(s) 76, 1257, 1674
TaqI TCGA 5 cut(s) 5, 67, 804, 1280, 1473
TatI WGTACW 1 cut(s) 903
TfiI GAWTC 5 cut(s) 118, 769, 1043, 2045, 2068
Tru1I TTAA 7 cut(s) 17, 51, 258, 864, 1065, 1686, 2091
Tru9I TTAA 7 cut(s) 17, 51, 258, 864, 1065, 1686, 2091
TscAI CASTG 6 cut(s) 919, 1005, 1233, 1634, 1839, 1856
TseFI GTSAC 5 cut(s) 880, 1112, 1454, 1788, 1893
TseI GCWGC 6 cut(s) 500, 605, 1829, 1877, 2438, 2441
Tsp45I GTSAC 5 cut(s) 880, 1112, 1454, 1788, 1893
TspGWI ACGGA 2 cut(s) 222, 1362
TspMI CCCGGG 1 cut(s) 1802
TspRI CASTG 6 cut(s) 919, 1005, 1233, 1634, 1839, 1856
Van91I CCANNNNNTGG 1 cut(s) 221
VpaK11BI GGWCC 3 cut(s) 782, 1583, 1948
VspI ATTAAT 2 cut(s) 1065, 1686
XapI RAATTY 8 cut(s) 793, 958, 1330, 1381, 1438, 1506, 1567, 2168
XbaI TCTAGA 1 cut(s) 104
XceI RCATGY 1 cut(s) 382
XmaI CCCGGG 1 cut(s) 1802
XmaJI CCTAGG 1 cut(s) 1364
XmiI GTMKAC 3 cut(s) 5, 1136, 1473
XmnI GAANNNNTTC 1 cut(s) 2358
Zsp2I ATGCAT 2 cut(s) 61, 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.