AT5G21150

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001623
Physical Location & Seq
Forward (+)
7192239 .. 7198381
6143 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G21150.1

Sequence Viewer

Length: 2691 bp
ATGGATTCTGATGAACCGAATGGGAGTGGATTACCACCTCCACCACCTTTCGTTCCAGCAAATCTTGTCCCTGAAGTGGAGCCTGTAAAAAAGAACATTCTTCTCCCAATGGCTCGGCCTCGAGGCAGCGGCTCCAAAGGACAGAAGATTCCTCTTCTTACTAATCACTTTGGAGTCAAGTTCAACAAACCAAGCGGTTACTTCTTTCATTACAGTGTTGCTATCAATTATGAAGATGGCCGTCCAGTGGAGGCTAAAGGTATCGGCCGAAAGATTCTTGACAAAGTTCAGGAGACCTATCAAAGTGATTTGGGTGCCAAATACTTTGCTTATGATGGCGAGAAGACTCTCTTCACTGTTGGTGCTCTTCCCAGCAACAAACTTGACTTCTCTGTTGTTCTTGAAGAAATACCTTCTAGCAGAAATCACGCTGGAAATGATACAAATGATGCTGATAGAAAAAGATCAAGGCGTCCCAACCAAACTAAGAAATTCATGGTTGAGATAAGTTATGCTGCAAAGATCCCCATGCAGGCTATTGCAAGCGCTCTTCAAGGGAAGGAGACAGAGAATCTTCAAGACGCTCTGAGAGTGCTGGATATTATTTTGCGCCAGAGTGCAGCTAGGCAAGGTTGCCTCCTTGTTCGCCAGTCCTTTTTCCACAATGACGTAAAGAACTTTGTACCTATTGGTGGAGGTGTCAGTGGTTGCAGAGGGTTCCATTCAAGTTTCAGAACTACTCAGGGAGGCTTATCCCTGAATATTGACACTTCAACTACGATGATAGTACAACCTGGACCTGTAGTTGATTTCCTGCTTGCTAACCAGAACAAGAAAGATCCATACGGAATGGACTGGAACAAGGCTCGACGTGTCCTCAAGAATCTGAGAGTTCAAATTACTCTTTCCAATAGAGAATACAAGATAAGTGGACTAAGTGAACACAGCTGCAAAGATCAACTATTTACATGGAGGAAACCTAACGACAAGGGAGAATTTGAGGAGGTTGAGATCACAGTGCTCAATTACTATAAAGAGCGTAACATTGAAGTGCGTTATTCAGGTGACTTCCCTTGCATCAATGTTGGTAAGCCGAAGCGTCCCACTTACTTCCCCATTGAGTTCTGTAATCTTGTGTCTCTACAGCGATACACAAAATCGCTTACCAATTTTCAGAGGGCTGCCCTAGTTGAAAAGTCTAGGCAGAAGCCACCTGAAAGGATGGCCTCGCTAACCAAAGGTCTGAAGGACAGCAATTACAATGCCGACCCGGTATTGCAAGATAGTGGTGTTAGCATTATCACCAATTTTACCCAAGTTGAAGGCCGTATCTTACCAACACCAATGCTGAAAGTGGGCAAGGGAGAAAACCTTTCCCCAATCAAAGGAAAATGGAACTTTATGCGTAAGACACTCGCTGAGCCAACGACGGTTACTAGATGGGCTGTTGTGAACTTCTCTGCTCGCTGTGATACAAATACACTTATTCGTGACTTGATTAAATGTGGACGGGAGAAAGGAATTAATGTAGAGCCTCCATTCAAGGATGTCATCAACGAGAATCCTCAGTTTAGGAATGCACCAGCTACTGTGAGAGTAGAGAATATGTTTGAGCAGATAAAATCCAAACTCCCAAAGCCGCCTCTGTTCCTTCTTTGCATACTCGCTGAAAGGAAAAACTCTGATGTTTATGGCCCTTGGAAAAAAAAGAATCTTGTTGATCTTGGAATTGTGACTCAGTGCATTGCTCCCACCAGACTGAACGATCAGTATCTCACCAATGTTCTCCTGAAGATAAATGCCAAGCTTGGTGGATTGAATTCGTTGTTAGCTATGGAGCGCTCACCAGCAATGCCAAAAGTAACGCAAGTTCCTACCATCATTGTTGGGATGGATGTATCCCATGGTTCCCCTGGCCAGTCTGATATACCATCAATTGCTGCTGTTGTGAGCTCAAGACAATGGCCACTCATCTCAAAATATAAGGCATGTGTACGCACACAATCACGCAAAATGGAAATGATTGATAATCTCTTCAAACCCGTCAATGGCAAAGACGAAGGAATGTTCAGGGAACTCTTGTTAGACTTTTACTACAGTTCAGAGAATAGGAAACCAGAGCACATCATTATTTTCAGGGATGGTGTAAGCGAGTCTCAGTTCAATCAAGTTCTTAATATTGAATTGGATCAGATGATGCAGGCATGCAAGTTTCTTGATGATACGTGGCATCCGAAGTTTACAGTGATAGTTGCCCAGAAGAACCACCACACAAAGTTCTTCCAGTCTCGAGGCCCTGATAATGTTCCTCCAGGAACAATCATTGACAGCCAGATCTGTCACCCACGCAACTTTGATTTCTATCTCTGCGCCCATGCTGGCATGATTGGAACTACAAGGCCAACACATTACCATGTTCTGTATGACGAGATTGGGTTTGCCACAGACGACCTCCAAGAACTTGTGCATTCTCTGTCCTATGTCTACCAGAGGAGCACCACTGCGATCTCAGTCGTTGCACCTGTATGTTACGCTCATTTGGCAGCTGCACAGATGGGAACTGTGATGAAGTATGAAGAGTTGTCTGAGACTTCTTCGAGCCATGGAGGAATCACCACACCTGGAGCAGTCCCTGTGCCACCTATGCCGCAGCTGCACAATAATGTTTCAACCTCCATGTTCTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003006 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0007049 GO:0007275 GO:0008150 GO:0008152 GO:0008213 GO:0009553 GO:0009554 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009791 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009908 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010528 GO:0010529 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022414 GO:0030154 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032501 GO:0032502 GO:0034293 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035670 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0043934 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044703 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048236 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048519 GO:0048523 GO:0048533 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051321 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061458 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0090567 GO:0097159 GO:0098542 GO:0099402 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903046 GO:1903506 GO:1903507 GO:1904159 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

896

Amino Acids

100.52

Weight (kDa)

9.18

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 48 - 203 9.2e-33 N-terminal domain of argonaute
ArgoL1 PF08699 215 - 263 1.5e-17 Argonaute linker 1 domain
PAZ PF02170 269 - 396 8.9e-28 PAZ domain
ArgoL2 PF16488 406 - 451 1.8e-12 Argonaute linker 2 domain
Piwi PF02171 551 - 857 5.2e-104 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 314
AccI GTMKAC 1 cut(s) 2484
AciI CCGC 4 cut(s) 129, 195, 1640, 2648
AclWI GGATC 3 cut(s) 517, 833, 2196
AcoI YGGCCR 4 cut(s) 238, 265, 1915, 1964
AcsI RAATTY 3 cut(s) 491, 995, 1819
AcuI CTGAAG 3 cut(s) 93, 1265, 1811
AcyI GRCGYC 1 cut(s) 472
AfaI GTAC 3 cut(s) 684, 789, 1995
AfeI AGCGCT 2 cut(s) 547, 1841
AfiI CCNNNNNNNGG 6 cut(s) 76, 247, 532, 692, 1385, 2048
AflIII ACRYGT 1 cut(s) 871
AjiI CACGTC 1 cut(s) 872
AjnI CCWGG 4 cut(s) 793, 1912, 2311, 2620
AluBI AGCT 8 cut(s) 623, 948, 1586, 1807, 1832, 1953, 2546, 2653
AluI AGCT 8 cut(s) 623, 948, 1586, 1807, 1832, 1953, 2546, 2653
Alw21I GWGCWC 5 cut(s) 367, 1023, 1955, 2124, 2498
Alw26I GTCTC 6 cut(s) 287, 557, 1143, 2160, 2292, 2582
AlwI GGATC 3 cut(s) 517, 833, 2196
AlwNI CAGNNNCTG 2 cut(s) 1589, 2633
Ama87I CYCGRG 2 cut(s) 120, 2289
Aor51HI AGCGCT 2 cut(s) 547, 1841
ApoI RAATTY 3 cut(s) 491, 995, 1819
AseI ATTAAT 1 cut(s) 1524
AspLEI GCGC 4 cut(s) 548, 612, 1842, 2372
AspS9I GGNCC 3 cut(s) 797, 1694, 2294
AsuC2I CCSGG 1 cut(s) 1271
AsuHPI GGTGA 6 cut(s) 1076, 1294, 1768, 1836, 2333, 2605
AvaI CYCGRG 2 cut(s) 120, 2289
AvaII GGWCC 1 cut(s) 797
BalI TGGCCA 2 cut(s) 1917, 1966
BanI GGYRCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 1955
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 5 cut(s) 367, 1023, 1955, 2124, 2498
BccI CCATC 9 cut(s) 230, 329, 1216, 1434, 1885, 1886, 1939, 2135, 2548
BceAI ACGGC 2 cut(s) 225, 1311
BciT130I CCWGG 4 cut(s) 795, 1914, 2313, 2622
BciVI GTATCC 1 cut(s) 1909
BcnI CCSGG 1 cut(s) 1271
BcoDI GTCTC 6 cut(s) 287, 557, 1143, 2160, 2292, 2582
BfaI CTAG 5 cut(s) 417, 624, 1187, 1200, 1437
BfmI CTRYAG 3 cut(s) 801, 1142, 2095
BfoI RGCGCY 2 cut(s) 549, 1843
BfuI GTATCC 1 cut(s) 1909
BglII AGATCT 1 cut(s) 2334
BlpI GCTNAGC 1 cut(s) 1419
Bme1390I CCNGG 5 cut(s) 795, 1271, 1914, 2313, 2622
Bme18I GGWCC 1 cut(s) 797
BmeT110I CYCGRG 2 cut(s) 120, 2289
BmgBI CACGTC 1 cut(s) 872
BmgT120I GGNCC 3 cut(s) 797, 1694, 2294
BmiI GGNNCC 5 cut(s) 81, 133, 316, 719, 1909
BmrFI CCNGG 5 cut(s) 795, 1271, 1914, 2313, 2622
BmsI GCATC 4 cut(s) 439, 1086, 2187, 2239
BpiI GAAGAC 1 cut(s) 350
BpmI CTGGAG 2 cut(s) 2295, 2643
Bpu1102I GCTNAGC 1 cut(s) 1419
BpuEI CTTGAG 2 cut(s) 863, 1939
BpuMI CCSGG 1 cut(s) 1271
BsaAI YACGTR 1 cut(s) 2226
BsaBI GATNNNNATC 2 cut(s) 1770, 2361
BsaHI GRCGYC 1 cut(s) 472
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 4 cut(s) 1697, 1903, 1912, 2602
Bsc4I CCNNNNNNNGG 6 cut(s) 76, 247, 532, 692, 1385, 2048
Bse1I ACTGG 5 cut(s) 245, 649, 860, 1918, 2284
Bse3DI GCAATG 2 cut(s) 1743, 1857
Bse8I GATNNNNATC 2 cut(s) 1770, 2361
BseBI CCWGG 4 cut(s) 795, 1914, 2313, 2622
BseDI CCNNGG 4 cut(s) 1697, 1903, 1912, 2602
BseGI GGATG 6 cut(s) 1227, 1552, 1896, 1900, 2146, 2230
BseJI GATNNNNATC 2 cut(s) 1770, 2361
BseLI CCNNNNNNNGG 6 cut(s) 76, 247, 532, 692, 1385, 2048
BseMI GCAATG 2 cut(s) 1743, 1857
BseMII CTCAG 9 cut(s) 578, 755, 878, 1410, 1580, 1751, 2171, 2523, 2577
BseNI ACTGG 5 cut(s) 245, 649, 860, 1918, 2284
BseRI GAGGAG 2 cut(s) 1016, 2506
BseX3I CGGCCG 1 cut(s) 265
BseYI CCCAGC 1 cut(s) 371
BsgI GTGCAG 3 cut(s) 639, 2532, 2639
Bsh1285I CGRYCG 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 5 cut(s) 367, 1023, 1955, 2124, 2498
BsiHKCI CYCGRG 2 cut(s) 120, 2289
BsiSI CCGG 1 cut(s) 1271
BslFI GGGAC 4 cut(s) 53, 459, 1086, 2615
BslI CCNNNNNNNGG 6 cut(s) 76, 247, 532, 692, 1385, 2048
BsmAI GTCTC 6 cut(s) 287, 557, 1143, 2160, 2292, 2582
BsmFI GGGAC 4 cut(s) 53, 459, 1086, 2615
BsmI GAATGC 2 cut(s) 1582, 2467
Bso31I GGTCTC 1 cut(s) 287
BsoBI CYCGRG 2 cut(s) 120, 2289
Bsp1286I GDGCHC 5 cut(s) 367, 1023, 1955, 2124, 2498
Bsp1720I GCTNAGC 1 cut(s) 1419
Bsp19I CCATGG 2 cut(s) 1903, 2602
BspACI CCGC 4 cut(s) 129, 195, 1640, 2648
BspCNI CTCAG 9 cut(s) 579, 754, 879, 1411, 1579, 1750, 2170, 2522, 2578
BspLI GGNNCC 5 cut(s) 81, 133, 316, 719, 1909
BspPI GGATC 3 cut(s) 517, 833, 2196
BspQI GCTCTTC 2 cut(s) 372, 555
BspT107I GGYRCC 1 cut(s) 314
BspTNI GGTCTC 1 cut(s) 287
BsrDI GCAATG 2 cut(s) 1743, 1857
BsrI ACTGG 5 cut(s) 245, 649, 860, 1918, 2284
BssECI CCNNGG 4 cut(s) 1697, 1903, 1912, 2602
BssNI GRCGYC 1 cut(s) 472
BssT1I CCWWGG 3 cut(s) 1697, 1903, 2602
Bst2UI CCWGG 4 cut(s) 795, 1914, 2313, 2622
Bst4CI ACNGT 8 cut(s) 215, 358, 1018, 1432, 1591, 2099, 2245, 2563
Bst6I CTCTTC 6 cut(s) 159, 356, 372, 555, 2039, 2571
BstACI GRCGYC 1 cut(s) 472
BstBAI YACGTR 1 cut(s) 2226
BstC8I GCNNGC 7 cut(s) 534, 544, 819, 1465, 2202, 2206, 2380
BstDSI CCRYGG 2 cut(s) 1903, 2602
BstF5I GGATG 6 cut(s) 1227, 1552, 1896, 1900, 2146, 2230
BstH2I RGCGCY 2 cut(s) 549, 1843
BstHHI GCGC 4 cut(s) 548, 612, 1842, 2372
BstMAI GTCTC 6 cut(s) 287, 557, 1143, 2160, 2292, 2582
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 3 cut(s) 2540, 2644, 2653
BstNI CCWGG 4 cut(s) 795, 1914, 2313, 2622
BstNSI RCATGY 2 cut(s) 1992, 2208
BstSCI CCNGG 5 cut(s) 793, 1269, 1912, 2311, 2620
BstSFI CTRYAG 3 cut(s) 801, 1142, 2095
BstV2I GAAGAC 1 cut(s) 350
BstX2I RGATCY 3 cut(s) 522, 838, 2334
BstYI RGATCY 3 cut(s) 522, 838, 2334
BstZI CGGCCG 1 cut(s) 265
BsuI GTATCC 1 cut(s) 1909
BtgI CCRYGG 2 cut(s) 1903, 2602
BtrI CACGTC 1 cut(s) 872
BtsCI GGATG 6 cut(s) 1227, 1552, 1896, 1900, 2146, 2230
BtsI GCAGTG 1 cut(s) 2499
BtsIMutI CAGTG 8 cut(s) 220, 252, 354, 709, 1023, 1745, 2250, 2499
Cac8I GCNNGC 7 cut(s) 534, 544, 819, 1465, 2202, 2206, 2380
CaiI CAGNNNCTG 2 cut(s) 1589, 2633
CfoI GCGC 4 cut(s) 548, 612, 1842, 2372
Cfr13I GGNCC 3 cut(s) 797, 1694, 2294
CseI GACGC 3 cut(s) 461, 590, 1088
Csp6I GTAC 3 cut(s) 683, 788, 1994
CspCI CAANNNNNGTGG 4 cut(s) 910, 945, 1792, 1827
CviQI GTAC 3 cut(s) 683, 788, 1994
EaeI YGGCCR 4 cut(s) 238, 265, 1915, 1964
EagI CGGCCG 1 cut(s) 265
Eam1104I CTCTTC 6 cut(s) 159, 356, 372, 555, 2039, 2571
EarI CTCTTC 6 cut(s) 159, 356, 372, 555, 2039, 2571
Ecl136II GAGCTC 1 cut(s) 1953
EclXI CGGCCG 1 cut(s) 265
Eco130I CCWWGG 3 cut(s) 1697, 1903, 2602
Eco24I GRGCYC 1 cut(s) 1955
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 1 cut(s) 797
Eco47III AGCGCT 2 cut(s) 547, 1841
Eco52I CGGCCG 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 1953
Eco57I CTGAAG 3 cut(s) 93, 1265, 1811
Eco88I CYCGRG 2 cut(s) 120, 2289
EcoICRI GAGCTC 1 cut(s) 1953
EcoO109I RGGNCCY 1 cut(s) 2294
EcoRI GAATTC 1 cut(s) 1819
EcoRII CCWGG 4 cut(s) 793, 1912, 2311, 2620
EcoT14I CCWWGG 3 cut(s) 1697, 1903, 2602
EcoT38I GRGCYC 1 cut(s) 1955
ErhI CCWWGG 3 cut(s) 1697, 1903, 2602
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 4 cut(s) 53, 459, 1086, 2615
FblI GTMKAC 1 cut(s) 2484
FokI GGATG 6 cut(s) 1234, 1559, 1903, 1907, 2153, 2217
FriOI GRGCYC 1 cut(s) 1955
FspBI CTAG 5 cut(s) 417, 624, 1187, 1200, 1437
GlaI GCGC 4 cut(s) 547, 611, 1841, 2371
GsaI CCCAGC 1 cut(s) 375
GsuI CTGGAG 2 cut(s) 2295, 2643
HaeII RGCGCY 2 cut(s) 549, 1843
HapII CCGG 1 cut(s) 1271
HgaI GACGC 3 cut(s) 461, 590, 1088
HhaI GCGC 4 cut(s) 548, 612, 1842, 2372
Hin1I GRCGYC 1 cut(s) 472
Hin6I GCGC 4 cut(s) 546, 610, 1840, 2370
HinP1I GCGC 4 cut(s) 546, 610, 1840, 2370
HindIII AAGCTT 1 cut(s) 1805
HpaII CCGG 1 cut(s) 1271
HphI GGTGA 6 cut(s) 1076, 1294, 1768, 1836, 2333, 2605
Hpy166II GTNNAC 7 cut(s) 932, 941, 1453, 1508, 1994, 2241, 2485
Hpy8I GTNNAC 7 cut(s) 932, 941, 1453, 1508, 1994, 2241, 2485
Hpy99I CGWCG 2 cut(s) 873, 1432
HpyAV CCTTC 6 cut(s) 423, 553, 1240, 1316, 1661, 2054
HpyCH4III ACNGT 8 cut(s) 215, 358, 1018, 1432, 1591, 2099, 2245, 2563
HpyCH4IV ACGT 3 cut(s) 669, 871, 2225
HpyF10VI GCNNNNNNNGC 3 cut(s) 2540, 2644, 2653
HpySE526I ACGT 3 cut(s) 669, 871, 2225
Hsp92I GRCGYC 1 cut(s) 472
HspAI GCGC 4 cut(s) 546, 610, 1840, 2370
LguI GCTCTTC 2 cut(s) 372, 555
LmnI GCTCC 6 cut(s) 79, 137, 1753, 1837, 2493, 2624
LweI GCATC 4 cut(s) 439, 1086, 2187, 2239
MaeI CTAG 5 cut(s) 417, 624, 1187, 1200, 1437
MaeII ACGT 3 cut(s) 669, 871, 2225
MaeIII GTNAC 9 cut(s) 197, 1040, 1064, 1432, 1490, 1732, 1861, 2339, 2528
MfeI CAATTG 1 cut(s) 1935
MflI RGATCY 3 cut(s) 522, 838, 2334
MhlI GDGCHC 5 cut(s) 367, 1023, 1955, 2124, 2498
MlsI TGGCCA 2 cut(s) 1917, 1966
MluNI TGGCCA 2 cut(s) 1917, 1966
MlyI GAGTC 4 cut(s) 183, 340, 1729, 2162
Mox20I TGGCCA 2 cut(s) 1917, 1966
MscI TGGCCA 2 cut(s) 1917, 1966
MseI TTAA 3 cut(s) 1500, 1524, 2175
MslI CAYNNNNRTG 5 cut(s) 213, 1049, 2412, 2524, 2661
Msp20I TGGCCA 2 cut(s) 1917, 1966
MspA1I CMGCKG 4 cut(s) 129, 948, 2546, 2653
MspI CCGG 1 cut(s) 1271
MspR9I CCNGG 5 cut(s) 795, 1271, 1914, 2313, 2622
MunI CAATTG 1 cut(s) 1935
Mva1269I GAATGC 2 cut(s) 1582, 2467
MvaI CCWGG 4 cut(s) 795, 1914, 2313, 2622
MwoI GCNNNNNNNGC 3 cut(s) 2540, 2644, 2653
NciI CCSGG 1 cut(s) 1271
NcoI CCATGG 2 cut(s) 1903, 2602
NlaIV GGNNCC 5 cut(s) 81, 133, 316, 719, 1909
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 4 cut(s) 1064, 1490, 1732, 2339
NspI RCATGY 2 cut(s) 1992, 2208
PaeI GCATGC 1 cut(s) 2208
PaeR7I CTCGAG 2 cut(s) 120, 2289
PciSI GCTCTTC 2 cut(s) 372, 555
PcsI WCGNNNNNNNCGW 1 cut(s) 2231
PctI GAATGC 2 cut(s) 1582, 2467
PfeI GAWTC 8 cut(s) 5, 148, 274, 571, 883, 1561, 1711, 2610
PfoI TCCNGGA 1 cut(s) 2311
PleI GAGTC 4 cut(s) 182, 340, 1729, 2161
PpsI GAGTC 4 cut(s) 182, 340, 1729, 2161
Ppu21I YACGTR 1 cut(s) 2226
PshBI ATTAAT 1 cut(s) 1524
Psp124BI GAGCTC 1 cut(s) 1955
Psp6I CCWGG 4 cut(s) 793, 1912, 2311, 2620
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 4 cut(s) 793, 1912, 2311, 2620
PspN4I GGNNCC 5 cut(s) 81, 133, 316, 719, 1909
PspPI GGNCC 3 cut(s) 797, 1694, 2294
PspXI VCTCGAGB 1 cut(s) 120
PsrI GAACNNNNNNTAC 2 cut(s) 1854, 1886
PstNI CAGNNNCTG 2 cut(s) 1589, 2633
PsuI RGATCY 3 cut(s) 522, 838, 2334
PvuII CAGCTG 3 cut(s) 948, 2546, 2653
RsaI GTAC 3 cut(s) 684, 789, 1995
RsaNI GTAC 3 cut(s) 683, 788, 1994
RseI CAYNNNNRTG 5 cut(s) 213, 1049, 2412, 2524, 2661
SacI GAGCTC 1 cut(s) 1955
SapI GCTCTTC 2 cut(s) 372, 555
SaqAI TTAA 3 cut(s) 1500, 1524, 2175
Sau96I GGNCC 3 cut(s) 797, 1694, 2294
SchI GAGTC 4 cut(s) 183, 340, 1729, 2162
ScrFI CCNGG 5 cut(s) 795, 1271, 1914, 2313, 2622
SduI GDGCHC 5 cut(s) 367, 1023, 1955, 2124, 2498
SfaNI GCATC 4 cut(s) 439, 1086, 2187, 2239
SfcI CTRYAG 3 cut(s) 801, 1142, 2095
Sfr274I CTCGAG 2 cut(s) 120, 2289
SinI GGWCC 1 cut(s) 797
SlaI CTCGAG 2 cut(s) 120, 2289
SmiMI CAYNNNNRTG 5 cut(s) 213, 1049, 2412, 2524, 2661
SmlI CTYRAG 4 cut(s) 120, 878, 1954, 2289
SmoI CTYRAG 4 cut(s) 120, 878, 1954, 2289
SphI GCATGC 1 cut(s) 2208
SsiI CCGC 4 cut(s) 129, 195, 1640, 2648
SspI AATATT 2 cut(s) 763, 2179
SspMI CTAG 5 cut(s) 417, 624, 1187, 1200, 1437
SstI GAGCTC 1 cut(s) 1955
StyD4I CCNGG 5 cut(s) 793, 1269, 1912, 2311, 2620
StyI CCWWGG 3 cut(s) 1697, 1903, 2602
TaaI ACNGT 8 cut(s) 215, 358, 1018, 1432, 1591, 2099, 2245, 2563
TaiI ACGT 3 cut(s) 672, 874, 2228
TaqI TCGA 4 cut(s) 121, 868, 2290, 2597
TatI WGTACW 1 cut(s) 787
TauI GCSGC 3 cut(s) 132, 1642, 2650
TfiI GAWTC 8 cut(s) 5, 148, 274, 571, 883, 1561, 1711, 2610
Tru1I TTAA 3 cut(s) 1500, 1524, 2175
Tru9I TTAA 3 cut(s) 1500, 1524, 2175
TscAI CASTG 8 cut(s) 220, 252, 361, 709, 1023, 1745, 2250, 2506
TseFI GTSAC 4 cut(s) 1064, 1490, 1732, 2339
Tsp45I GTSAC 4 cut(s) 1064, 1490, 1732, 2339
TspDTI ATGAA 6 cut(s) 27, 197, 246, 484, 2582, 2589
TspGWI ACGGA 1 cut(s) 861
TspRI CASTG 8 cut(s) 220, 252, 361, 709, 1023, 1745, 2250, 2506
VpaK11BI GGWCC 1 cut(s) 797
VspI ATTAAT 1 cut(s) 1524
XapI RAATTY 3 cut(s) 491, 995, 1819
XceI RCATGY 2 cut(s) 1992, 2208
XcmI CCANNNNNNNNNTGG 1 cut(s) 1910
XhoI CTCGAG 2 cut(s) 120, 2289
XmiI GTMKAC 1 cut(s) 2484
XspI CTAG 5 cut(s) 417, 624, 1187, 1200, 1437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.