Rorug01G0088700

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001623
Physical Location & Seq
Forward (+)
14415588 .. 14416397
810 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0088700.1

Sequence Viewer

Length: 810 bp
ATGGAACCACAAACTCTGCAACTAATGGAGCTACAAACCCAGCAACAAATCGAGGATGTAGGAAACAACCAAGCAGCCGGGAGTAGTGCCAACACGGATTTCTGGCGAAGCCGTACCAGGTGGATTCCTACTCCAGATCAAATAAGAATCCTCAAGGATCTTTACTACGACAAGGGAGTTAAGTGCCCAACTACAGAGCACATTCACGAGATCTGTCTCCAGCTGAACCAGTATGGACAGGTTGAGGGCAAGAACTTTTACTTTTGGTTCCAGAACGTCAGGGCTCGAGAGAAGCAGATGAAGAGGTGCAATCAGGCTGCTCAAGTGCCCATGGGAACTAGTTCTCCTGGTACTGGTGGATCCATTGATCTCAATTTTGGGTCCACTGGTTCTACTTGTGCTGGTGGATCCATCGACATCAATTTTGGGCCAGCTGGTGGATCCATTGACATCAATTTTGGGTCCACTAGTTCTACTGGTGCTGGTGGATCCATTGATCTCAATTTTGGGCCATGGGTCGACTGCAATGTTGACCCGTATTTCTCCTCACCCTTCACTACTAGTACTACTTTCTTTGGCAGGAATGAAGAACAAACTTCCATGGAACAACGAGGAGAAGATTACCAGGAGATTGAAACTCTTCCTTTGTTCCCCATGCCCGGTGAGGACATCCTTGGCAACCTAAAGACTACTTCCGAGGGAGGTAGCTGCTCTGGCTACTATTCTGGTGGCTCGGACGGCTACGACGGTGGCTCTCACATTTCCCTTGAGCTCAGCCTCAACCCCTCCGGGGCTGCTGACTTGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

269

Amino Acids

29.13

Weight (kDa)

4.43

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 38 - 99 6.4e-08 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 437
AccI GTMKAC 1 cut(s) 519
AclWI GGATC 9 cut(s) 165, 354, 367, 402, 415, 435, 448, 483, 496
AfaI GTAC 3 cut(s) 115, 352, 565
AfiI CCNNNNNNNGG 4 cut(s) 353, 437, 659, 790
AgsI TTSAA 1 cut(s) 635
AhlI ACTAGT 3 cut(s) 338, 467, 560
AjnI CCWGG 3 cut(s) 116, 346, 624
AjuI GAANNNNNNNTTGG 2 cut(s) 83, 115
AloI GAACNNNNNNTCC 2 cut(s) 328, 360
AluBI AGCT 5 cut(s) 31, 223, 434, 708, 772
AluI AGCT 5 cut(s) 31, 223, 434, 708, 772
Alw21I GWGCWC 2 cut(s) 201, 774
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 9 cut(s) 165, 354, 367, 402, 415, 435, 448, 483, 496
Ama87I CYCGRG 1 cut(s) 285
AoxI GGCC 2 cut(s) 428, 509
ApeKI GCWGC 4 cut(s) 74, 317, 708, 794
ArsI GACNNNNNNTTYG 4 cut(s) 407, 439, 440, 472
Asp700I GAANNNNTTC 2 cut(s) 340, 639
AspS9I GGNCC 4 cut(s) 381, 428, 462, 509
AsuC2I CCSGG 3 cut(s) 79, 660, 790
AsuHPI GGTGA 2 cut(s) 540, 674
AvaI CYCGRG 1 cut(s) 285
AvaII GGWCC 2 cut(s) 381, 462
BaeGI GKGCMC 2 cut(s) 188, 330
BamHI GGATCC 4 cut(s) 359, 407, 440, 488
BanII GRGCYC 2 cut(s) 286, 774
BauI CACGAG 1 cut(s) 206
Bbv12I GWGCWC 2 cut(s) 201, 774
BbvI GCAGC 4 cut(s) 86, 304, 695, 781
BccI CCATC 1 cut(s) 419
BceAI ACGGC 2 cut(s) 96, 754
BciT130I CCWGG 3 cut(s) 118, 348, 626
BcnI CCSGG 3 cut(s) 79, 660, 790
BcoDI GTCTC 1 cut(s) 221
BcuI ACTAGT 3 cut(s) 338, 467, 560
BfaI CTAG 3 cut(s) 339, 468, 561
BfmI CTRYAG 1 cut(s) 192
BglII AGATCT 1 cut(s) 210
BisI GCNGC 4 cut(s) 75, 318, 709, 795
BlpI GCTNAGC 1 cut(s) 773
BlsI GCNGC 4 cut(s) 76, 319, 710, 796
BmcAI AGTACT 1 cut(s) 565
Bme1390I CCNGG 6 cut(s) 79, 118, 348, 626, 660, 790
Bme18I GGWCC 2 cut(s) 381, 462
BmeT110I CYCGRG 1 cut(s) 285
BmgT120I GGNCC 4 cut(s) 381, 428, 462, 509
BmiI GGNNCC 8 cut(s) 6, 269, 361, 382, 409, 442, 463, 490
BmrFI CCNGG 6 cut(s) 79, 118, 348, 626, 660, 790
BpmI CTGGAG 2 cut(s) 117, 203
Bpu1102I GCTNAGC 1 cut(s) 773
BpuEI CTTGAG 3 cut(s) 137, 306, 788
BpuMI CCSGG 3 cut(s) 79, 660, 790
BsaJI CCNNGG 6 cut(s) 330, 512, 600, 673, 696, 789
BsaXI ACNNNNNCTCC 2 cut(s) 328, 358
Bsc4I CCNNNNNNNGG 4 cut(s) 353, 437, 659, 790
Bse1I ACTGG 4 cut(s) 229, 358, 391, 481
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 3 cut(s) 118, 348, 626
BseDI CCNNGG 6 cut(s) 330, 512, 600, 673, 696, 789
BseGI GGATG 2 cut(s) 61, 669
BseLI CCNNNNNNNGG 4 cut(s) 353, 437, 659, 790
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 1 cut(s) 787
BseNI ACTGG 4 cut(s) 229, 358, 391, 481
BseRI GAGGAG 2 cut(s) 535, 627
BseSI GKGCMC 2 cut(s) 188, 330
BseXI GCAGC 4 cut(s) 86, 304, 695, 781
BseYI CCCAGC 1 cut(s) 39
BshFI GGCC 2 cut(s) 430, 511
BsiHKAI GWGCWC 2 cut(s) 201, 774
BsiHKCI CYCGRG 1 cut(s) 285
BsiSI CCGG 3 cut(s) 78, 660, 789
BslI CCNNNNNNNGG 4 cut(s) 353, 437, 659, 790
BsmAI GTCTC 1 cut(s) 221
BsnI GGCC 2 cut(s) 430, 511
BsoBI CYCGRG 1 cut(s) 285
Bsp1286I GDGCHC 5 cut(s) 188, 201, 286, 330, 774
Bsp143I GATC 9 cut(s) 136, 157, 210, 359, 367, 407, 440, 488, 496
Bsp1720I GCTNAGC 1 cut(s) 773
Bsp19I CCATGG 3 cut(s) 330, 512, 600
BspANI GGCC 2 cut(s) 430, 511
BspCNI CTCAG 1 cut(s) 786
BspLI GGNNCC 8 cut(s) 6, 269, 361, 382, 409, 442, 463, 490
BspPI GGATC 9 cut(s) 165, 354, 367, 402, 415, 435, 448, 483, 496
BsrDI GCAATG 1 cut(s) 532
BsrI ACTGG 4 cut(s) 229, 358, 391, 481
BssECI CCNNGG 6 cut(s) 330, 512, 600, 673, 696, 789
BssMI GATC 9 cut(s) 136, 157, 210, 359, 367, 407, 440, 488, 496
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 4 cut(s) 330, 512, 600, 673
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 3 cut(s) 118, 348, 626
Bst4CI ACNGT 1 cut(s) 749
Bst6I CTCTTC 2 cut(s) 296, 645
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 2 cut(s) 773, 807
BstDSI CCRYGG 3 cut(s) 330, 512, 600
BstF5I GGATG 2 cut(s) 61, 669
BstKTI GATC 9 cut(s) 139, 160, 213, 362, 370, 410, 443, 491, 499
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 9 cut(s) 136, 157, 210, 359, 367, 407, 440, 488, 496
BstMWI GCNNNNNNNGC 3 cut(s) 714, 738, 803
BstNI CCWGG 3 cut(s) 118, 348, 626
BstSCI CCNGG 6 cut(s) 77, 116, 346, 624, 658, 788
BstSFI CTRYAG 1 cut(s) 192
BstSLI GKGCMC 2 cut(s) 188, 330
BstV1I GCAGC 4 cut(s) 86, 304, 695, 781
BstX2I RGATCY 6 cut(s) 157, 210, 359, 407, 440, 488
BstYI RGATCY 6 cut(s) 157, 210, 359, 407, 440, 488
BsuRI GGCC 2 cut(s) 430, 511
BtgI CCRYGG 3 cut(s) 330, 512, 600
BtsCI GGATG 2 cut(s) 61, 669
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 1 cut(s) 432
Cfr13I GGNCC 4 cut(s) 381, 428, 462, 509
CsiI ACCWGGT 1 cut(s) 116
Csp6I GTAC 3 cut(s) 114, 351, 564
CviAII CATG 4 cut(s) 331, 513, 601, 655
CviQI GTAC 3 cut(s) 114, 351, 564
DdeI CTNAG 2 cut(s) 773, 807
DpnI GATC 9 cut(s) 138, 159, 212, 361, 369, 409, 442, 490, 498
DpnII GATC 9 cut(s) 136, 157, 210, 359, 367, 407, 440, 488, 496
Eam1104I CTCTTC 2 cut(s) 296, 645
EarI CTCTTC 2 cut(s) 296, 645
Ecl136II GAGCTC 1 cut(s) 772
Eco130I CCWWGG 4 cut(s) 330, 512, 600, 673
Eco24I GRGCYC 2 cut(s) 286, 774
Eco47I GGWCC 2 cut(s) 381, 462
Eco53kI GAGCTC 1 cut(s) 772
Eco88I CYCGRG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 772
EcoRII CCWGG 3 cut(s) 116, 346, 624
EcoT14I CCWWGG 4 cut(s) 330, 512, 600, 673
EcoT38I GRGCYC 2 cut(s) 286, 774
ErhI CCWWGG 4 cut(s) 330, 512, 600, 673
FaeI CATG 4 cut(s) 334, 516, 604, 658
FaiI YATR 5 cut(s) 234, 332, 514, 602, 656
FatI CATG 4 cut(s) 330, 512, 600, 654
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 75, 318, 709, 795
FokI GGATG 2 cut(s) 68, 656
FriOI GRGCYC 2 cut(s) 286, 774
Fsp4HI GCNGC 4 cut(s) 75, 318, 709, 795
FspBI CTAG 3 cut(s) 339, 468, 561
GluI GCNGC 4 cut(s) 75, 318, 709, 795
GsaI CCCAGC 1 cut(s) 43
GsuI CTGGAG 2 cut(s) 117, 203
HaeIII GGCC 2 cut(s) 430, 511
HapII CCGG 3 cut(s) 78, 660, 789
Hin1II CATG 4 cut(s) 334, 516, 604, 658
HincII GTYRAC 2 cut(s) 520, 532
HindII GTYRAC 2 cut(s) 520, 532
HinfI GANTC 2 cut(s) 124, 147
HpaII CCGG 3 cut(s) 78, 660, 789
HphI GGTGA 2 cut(s) 540, 674
Hpy166II GTNNAC 4 cut(s) 384, 465, 520, 532
Hpy188I TCNGA 2 cut(s) 697, 736
Hpy188III TCNNGA 4 cut(s) 134, 206, 271, 287
Hpy8I GTNNAC 4 cut(s) 384, 465, 520, 532
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 1 cut(s) 562
HpyCH4III ACNGT 1 cut(s) 749
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 19, 309, 525
HpyF10VI GCNNNNNNNGC 3 cut(s) 714, 738, 803
HpyF3I CTNAG 2 cut(s) 773, 807
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 4 cut(s) 334, 516, 604, 658
Kzo9I GATC 9 cut(s) 136, 157, 210, 359, 367, 407, 440, 488, 496
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 4 cut(s) 86, 304, 695, 781
MabI ACCWGGT 1 cut(s) 116
MaeI CTAG 3 cut(s) 339, 468, 561
MaeII ACGT 1 cut(s) 276
MalI GATC 9 cut(s) 138, 159, 212, 361, 369, 409, 442, 490, 498
MboI GATC 9 cut(s) 136, 157, 210, 359, 367, 407, 440, 488, 496
MboII GAAGA 4 cut(s) 313, 599, 629, 632
MflI RGATCY 6 cut(s) 157, 210, 359, 407, 440, 488
MhlI GDGCHC 5 cut(s) 188, 201, 286, 330, 774
MluCI AATT 4 cut(s) 373, 421, 454, 502
MroXI GAANNNNTTC 2 cut(s) 340, 639
MseI TTAA 1 cut(s) 180
MspA1I CMGCKG 2 cut(s) 223, 434
MspI CCGG 3 cut(s) 78, 660, 789
MspR9I CCNGG 6 cut(s) 79, 118, 348, 626, 660, 790
MvaI CCWGG 3 cut(s) 118, 348, 626
MwoI GCNNNNNNNGC 3 cut(s) 714, 738, 803
NciI CCSGG 3 cut(s) 79, 660, 790
NcoI CCATGG 3 cut(s) 330, 512, 600
NdeII GATC 9 cut(s) 136, 157, 210, 359, 367, 407, 440, 488, 496
NlaIII CATG 4 cut(s) 334, 516, 604, 658
NlaIV GGNNCC 8 cut(s) 6, 269, 361, 382, 409, 442, 463, 490
PaeR7I CTCGAG 1 cut(s) 285
PdmI GAANNNNTTC 2 cut(s) 340, 639
PfeI GAWTC 2 cut(s) 124, 147
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 4 cut(s) 76, 319, 710, 796
Psp124BI GAGCTC 1 cut(s) 774
Psp6I CCWGG 3 cut(s) 116, 346, 624
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 3 cut(s) 116, 346, 624
PspN4I GGNNCC 8 cut(s) 6, 269, 361, 382, 409, 442, 463, 490
PspPI GGNCC 4 cut(s) 381, 428, 462, 509
PsuI RGATCY 6 cut(s) 157, 210, 359, 407, 440, 488
PvuII CAGCTG 2 cut(s) 223, 434
RsaI GTAC 3 cut(s) 115, 352, 565
RsaNI GTAC 3 cut(s) 114, 351, 564
SacI GAGCTC 1 cut(s) 774
SalI GTCGAC 1 cut(s) 518
SaqAI TTAA 1 cut(s) 180
SatI GCNGC 4 cut(s) 75, 318, 709, 795
Sau3AI GATC 9 cut(s) 136, 157, 210, 359, 367, 407, 440, 488, 496
Sau96I GGNCC 4 cut(s) 381, 428, 462, 509
ScaI AGTACT 1 cut(s) 565
ScrFI CCNGG 6 cut(s) 79, 118, 348, 626, 660, 790
SduI GDGCHC 5 cut(s) 188, 201, 286, 330, 774
SexAI ACCWGGT 1 cut(s) 116
SfcI CTRYAG 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 285
SinI GGWCC 2 cut(s) 381, 462
SlaI CTCGAG 1 cut(s) 285
SmlI CTYRAG 4 cut(s) 152, 285, 321, 767
SmoI CTYRAG 4 cut(s) 152, 285, 321, 767
SpeI ACTAGT 3 cut(s) 338, 467, 560
Sse9I AATT 4 cut(s) 373, 421, 454, 502
SspMI CTAG 3 cut(s) 339, 468, 561
SstI GAGCTC 1 cut(s) 774
StyD4I CCNGG 6 cut(s) 77, 116, 346, 624, 658, 788
StyI CCWWGG 4 cut(s) 330, 512, 600, 673
TaaI ACNGT 1 cut(s) 749
TaiI ACGT 1 cut(s) 279
TaqI TCGA 4 cut(s) 51, 286, 414, 519
TasI AATT 4 cut(s) 373, 421, 454, 502
TatI WGTACW 1 cut(s) 563
TfiI GAWTC 2 cut(s) 124, 147
Tru1I TTAA 1 cut(s) 180
Tru9I TTAA 1 cut(s) 180
TscAI CASTG 1 cut(s) 391
TseI GCWGC 4 cut(s) 74, 317, 708, 794
TspDTI ATGAA 2 cut(s) 314, 600
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 381, 462
XhoI CTCGAG 1 cut(s) 285
XmiI GTMKAC 1 cut(s) 519
XmnI GAANNNNTTC 2 cut(s) 340, 639
XspI CTAG 3 cut(s) 339, 468, 561
ZrmI AGTACT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.