MD07G1052200.v1.1

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001623
Physical Location & Seq
Forward (+)
4819066 .. 4827543
8478 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1052200.v1.1.491

Sequence Viewer

Length: 2601 bp
ATGGCAAGGAGTGGGAATGGAACTAAAGGGCAGAAGATCCCTCTTCTGACCAACCATTTCAAAGTGGCAGTGAACAAGTCTGATGAGTACTTTTTCCATTACAGTATTGCGATGTCTTATGAAGATGGTACCCCCGTCGATGGGAAAGGCATTGGACGAAAGGTCATTGATAAAGTGAAACAGACATACGCAATGGAGCTTGCCGATAAAGAATTTGCTTATGACGGAGAGAAAAGCTTGTTTACCGTTGGCCCTCTTCCTCACAACAGACTTGAGTTCACAGTTGTACTGGATGACATCTCATCGACCAGGAGGACTGCAAGCCCAGGAGGTGGCTCCAACCGTGCTGACAGTGAGGGAGATCGGAAGAGAGTTAAGAAGCAATTTCAATCCAAACAATTAAATGTGCTGATCAACTATGCTACTAAAATCCCAATGCAAGCAATCGTGAATGCAATACGTGGCCAGGATTCAGAACATTTTCAGGAAGCGGTGAGGGTTCTTGATATCGTACTGAGGCAGAATGCAGCGAAACAGGGTTGTCTTCTTGTCCGACAGTCTTTCTTCCACAACAATCCAAGGAACTTCGCGGACTTGGGATCAGGTGTGTTAGGCTGCAGGGGTTTTCATTCAAGTTTCCGAGCCACCCAAGGGGGTCTATCTCTTAATATGGACGTATCCACTACAATGATTGTAAAACCTGGCCCCGTTCTAGACTTTCTGATGCTAAACCAGAACGTTAAAACTCCTTACCAGATCGATTGGACGAAGGCTAAGAGAATGCTGAAAAATCTGAGGATCACAACCTATTCATCTAAGATGGAGTACAAAATCACTGGACTGAGTGATAGACCATGCAAAGAGCAGAGGTTCTTCCTGAAATCGAAAAAAGGACAGGATGGTGATGGAGAAGAAATTACGGTTTCTAAATATTTTGCTGTCTATAAAAACCTACCATTGCGGGATTCAGCAGACTTCCCATGCATCAATGTTGGGAAACCAAAGAAGCCATCTTACTTTCCACTTGAGCTTTGCAACTTGGTTTCACTGCAACGCTATACAAAATCTTTAACCAATTTGCAAAGGGCTTCACTAGTGGAGAAGTCTCGGCAGAAGCCCCAGGAGAGGATGTCAGTTTTGCGTGATGCACTGAGGACTAGTAATTATGATGCTGACCTGATGCTTCGTTCTTCTGGAGTGTCCATTGGTGTTGACTTTGTGCATGTTGAAGGCCGTGTATTGCCAGCTCCAAAGCTAAAAGTTGGGAACGGAGAGGACTTTTTTCCGCGCAACGGGAGGTGGAATTTTAACAACAAGAAACTGGTACAACCTGTGACTATAGATCGTTGGGCTATTGTCAATTTCTCTGCTCGCTGTGACACTCGGAACCTGGTCAGCAATATGATGAAGTGCGGAAATATGAAAGGAATTGCCATAAAGGAACCGTTTATGGTATTTGATGAGAGTAACCAGCACAGACGTGAACCAGCTCCCGTTAGAGTGGATAAGATGTTTGAATACATAAAGTCAAAACTTCCAGGACCACCTCAACTTCTGTTGTGTATTCTTCCTGAGAGGAAGAATTCTGACATATATGGTCCATGGAAAAGGAAGAATCTTTCTGAGCTTGGGATTGTAACACAGTGCATTGCTCCTACAAAGGTGAATGATCAGTACATTACAAATGTGCTACTGAAAATCAATGCAAAGATGGGTGGAATGAATTCGTTGCTAAGTGTGGAGCATTCTCCTTCTATTCCATTGGTTTCCAAGCGCCCCACTCTGATTCTCGGTATGGATGTGTCACACGGCTCACCTGGGCGGTCAGACGTGCCTTCTATTGCAGCGGTGGTGAGTTCCAGGCACTGGCCCTTGATTTCTCGTTACCGAGCTGCTGTGCGTACTCAATCACCAAAAGTGGAAATGATAGCTTCTCTGTTCAAGCCTGTGTCAGATAAAGAGGATGATGGCATAATCAGGGAACTGTTGGTAGACTTTTATGCTACTTCAGGAAGCAGGAAGCCGGATCAGATAATTATTTTCAGGGATGGAGTGAGTGAATCACAGTTCAACCAAGTCTTGAATGTGGAACTGGATCAGATTATTGAGGCCTGCAAATTTCTTGATGAGACTTGGTCTCCAAAGTTCATGTTGATTGTTGCACAAAAGAATCATCACACCAAGTTCTTCCAGACGCATTCACCTGACAATGTTCCACCGGGAACCATCATAGACAACAAAGTCTGTCACCCCAAGAACAATGATTTCTACTTGTGCTCTCATGCTGGGATGATTGGGACTACTCGGCCTACCCATTATCATGTTCTGTATGATGAACTTGGGTTTTCAACGGATGACTTGCAAGAATTGGTTCATTCTCTGTCTTATGTGTACCAAAGAAGCACGACAGCCATATCCGTAGTTGCTCCAATCTGCTACGCCCATCTAGCTGCTGCCCAGATTTCCCAGTTCATCAAGTTTGATGAAATGTCGGAGACTGCCTCAAGCCATGGTGGTGGAATCACAGTTCCAGGTGCTGTTCCGGTGCCCGAGTTACCCAAGCTGCATAAAAATGTGATCAACTCCATGTTCTTCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

867

Amino Acids

97.19

Weight (kDa)

9.37

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 11 - 172 2.1e-27 N-terminal domain of argonaute
ArgoL1 PF08699 184 - 233 2.8e-18 Argonaute linker 1 domain
PAZ PF02170 238 - 365 1.3e-24 PAZ domain
ArgoL2 PF16488 375 - 420 7.3e-09 Argonaute linker 2 domain
ArgoMid PF16487 429 - 481 1.2e-06 Mid domain of argonaute
Piwi PF02171 519 - 826 3.6e-107 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2240
Acc65I GGTACC 1 cut(s) 128
AccB1I GGYRCC 2 cut(s) 128, 2545
AccB7I CCANNNNNTGG 2 cut(s) 332, 1866
AccI GTMKAC 1 cut(s) 1992
AccII CGCG 2 cut(s) 590, 1288
AciI CCGC 7 cut(s) 491, 590, 961, 1286, 1413, 1822, 1847
AclI AACGTT 1 cut(s) 738
AclWI GGATC 5 cut(s) 31, 607, 806, 2034, 2103
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 5 cut(s) 212, 1303, 1582, 1723, 2117
AcuI CTGAAG 1 cut(s) 1992
AfaI GTAC 9 cut(s) 89, 130, 288, 513, 827, 1326, 1676, 1903, 2393
AfiI CCNNNNNNNGG 7 cut(s) 140, 141, 332, 651, 1125, 1292, 1866
AgsI TTSAA 9 cut(s) 61, 389, 633, 1229, 1517, 1942, 2071, 2083, 2349
AhdI GACNNNNNGTC 1 cut(s) 161
AhlI ACTAGT 2 cut(s) 1093, 1157
AjiI CACGTC 2 cut(s) 1481, 1831
AleI CACNNNNGTG 1 cut(s) 1479
AloI GAACNNNNNNTCC 2 cut(s) 2511, 2543
Alw21I GWGCWC 1 cut(s) 2279
Alw26I GTCTC 4 cut(s) 1110, 2123, 2142, 2489
AlwI GGATC 5 cut(s) 31, 607, 806, 2034, 2103
AlwNI CAGNNNCTG 2 cut(s) 1866, 2537
Ama87I CYCGRG 1 cut(s) 2549
AoxI GGCC 7 cut(s) 250, 463, 703, 1231, 1868, 2109, 2306
ApeKI GCWGC 7 cut(s) 527, 615, 1844, 1892, 2450, 2453, 2563
ApoI RAATTY 5 cut(s) 212, 1303, 1582, 1723, 2117
Asp700I GAANNNNTTC 3 cut(s) 480, 1723, 2370
Asp718I GGTACC 1 cut(s) 128
AspLEI GCGC 2 cut(s) 1290, 1776
AspS9I GGNCC 5 cut(s) 251, 704, 1541, 1598, 1869
AsuC2I CCSGG 1 cut(s) 2220
AsuHPI GGTGA 8 cut(s) 505, 914, 1675, 1806, 1864, 1902, 2193, 2240
AvaI CYCGRG 1 cut(s) 2549
AvaII GGWCC 2 cut(s) 1541, 1598
BaeGI GKGCMC 1 cut(s) 2550
BaeI ACNNNNGTAYC 2 cut(s) 120, 153
BalI TGGCCA 1 cut(s) 465
BanI GGYRCC 2 cut(s) 128, 2545
BarI GAAGNNNNNNTAC 2 cut(s) 998, 1030
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 2279
BbvI GCAGC 7 cut(s) 539, 602, 1856, 1879, 2437, 2440, 2550
BceAI ACGGC 2 cut(s) 1218, 1825
BciVI GTATCC 1 cut(s) 688
BclI TGATCA 3 cut(s) 411, 1669, 2577
BcnI CCSGG 1 cut(s) 2220
BcoDI GTCTC 4 cut(s) 1110, 2123, 2142, 2489
BcuI ACTAGT 2 cut(s) 1093, 1157
BfaI CTAG 4 cut(s) 713, 1094, 1158, 2447
BfmI CTRYAG 2 cut(s) 616, 1338
BfoI RGCGCY 1 cut(s) 1777
BfuI GTATCC 1 cut(s) 688
BisI GCNGC 7 cut(s) 528, 616, 1845, 1893, 2451, 2454, 2564
BlsI GCNGC 7 cut(s) 529, 617, 1846, 1894, 2452, 2455, 2565
BmcAI AGTACT 1 cut(s) 89
Bme18I GGWCC 2 cut(s) 1541, 1598
BmeRI GACNNNNNGTC 1 cut(s) 161
BmeT110I CYCGRG 1 cut(s) 2549
BmgBI CACGTC 2 cut(s) 1481, 1831
BmgT120I GGNCC 5 cut(s) 251, 704, 1541, 1598, 1869
BmiI GGNNCC 7 cut(s) 130, 337, 706, 1388, 1443, 2224, 2547
BmrI ACTGGG 1 cut(s) 2461
BmsI GCATC 5 cut(s) 714, 993, 1135, 1159, 1170
BmuI ACTGGG 1 cut(s) 2461
BpiI GAAGAC 1 cut(s) 536
BplI GAGNNNNNCTC 2 cut(s) 2486, 2518
BpmI CTGGAG 1 cut(s) 1215
BpuEI CTTGAG 3 cut(s) 293, 1046, 2488
BpuMI CCSGG 1 cut(s) 2220
Bsa29I ATCGAT 1 cut(s) 759
BsaAI YACGTR 1 cut(s) 461
BsaI GGTCTC 1 cut(s) 2142
BsaJI CCNNGG 7 cut(s) 325, 578, 649, 1119, 1601, 1817, 2509
BsaWI WCCGGW 1 cut(s) 2542
BsaXI ACNNNNNCTCC 4 cut(s) 1115, 1145, 2122, 2152
Bsc4I CCNNNNNNNGG 7 cut(s) 140, 141, 332, 651, 1125, 1292, 1866
Bse1I ACTGG 6 cut(s) 294, 841, 1326, 1871, 2097, 2467
Bse3DI GCAATG 3 cut(s) 198, 956, 1647
BseCI ATCGAT 1 cut(s) 759
BseDI CCNNGG 7 cut(s) 325, 578, 649, 1119, 1601, 1817, 2509
BseGI GGATG 8 cut(s) 298, 904, 1134, 1804, 1969, 2053, 2295, 2359
BseLI CCNNNNNNNGG 7 cut(s) 140, 141, 332, 651, 1125, 1292, 1866
BseMI GCAATG 3 cut(s) 198, 956, 1647
BseMII CTCAG 6 cut(s) 506, 785, 833, 1142, 1563, 1614
BseNI ACTGG 6 cut(s) 294, 841, 1326, 1871, 2097, 2467
BseSI GKGCMC 1 cut(s) 2550
BseXI GCAGC 7 cut(s) 539, 602, 1856, 1879, 2437, 2440, 2550
BseYI CCCAGC 1 cut(s) 2285
Bsh1236I CGCG 2 cut(s) 590, 1288
BshFI GGCC 7 cut(s) 252, 465, 705, 1233, 1870, 2111, 2308
BshNI GGYRCC 2 cut(s) 128, 2545
BshVI ATCGAT 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 2279
BsiHKCI CYCGRG 1 cut(s) 2549
BsiSI CCGG 3 cut(s) 2024, 2219, 2543
BslFI GGGAC 1 cut(s) 2311
BslI CCNNNNNNNGG 7 cut(s) 140, 141, 332, 651, 1125, 1292, 1866
BsmAI GTCTC 4 cut(s) 1110, 2123, 2142, 2489
BsmFI GGGAC 1 cut(s) 2311
BsmI GAATGC 5 cut(s) 457, 529, 786, 1744, 2197
BsnI GGCC 7 cut(s) 252, 465, 705, 1233, 1870, 2111, 2308
Bso31I GGTCTC 1 cut(s) 2142
BsoBI CYCGRG 1 cut(s) 2549
Bsp1286I GDGCHC 2 cut(s) 2279, 2550
Bsp19I CCATGG 2 cut(s) 1601, 2509
BspACI CCGC 7 cut(s) 491, 590, 961, 1286, 1413, 1822, 1847
BspANI GGCC 7 cut(s) 252, 465, 705, 1233, 1870, 2111, 2308
BspCNI CTCAG 6 cut(s) 507, 786, 834, 1143, 1564, 1615
BspDI ATCGAT 1 cut(s) 759
BspFNI CGCG 2 cut(s) 590, 1288
BspLI GGNNCC 7 cut(s) 130, 337, 706, 1388, 1443, 2224, 2547
BspMAI CTGCAG 1 cut(s) 620
BspPI GGATC 5 cut(s) 31, 607, 806, 2034, 2103
BspT107I GGYRCC 2 cut(s) 128, 2545
BspTNI GGTCTC 1 cut(s) 2142
BsrDI GCAATG 3 cut(s) 198, 956, 1647
BsrI ACTGG 6 cut(s) 294, 841, 1326, 1871, 2097, 2467
BssECI CCNNGG 7 cut(s) 325, 578, 649, 1119, 1601, 1817, 2509
BssT1I CCWWGG 4 cut(s) 578, 649, 1601, 2509
Bst6I CTCTTC 3 cut(s) 48, 261, 362
BstBAI YACGTR 1 cut(s) 461
BstC8I GCNNGC 6 cut(s) 201, 322, 441, 1245, 1372, 2113
BstDEI CTNAG 9 cut(s) 515, 774, 794, 816, 842, 1151, 1572, 1623, 1733
BstDSI CCRYGG 2 cut(s) 1601, 2509
BstF5I GGATG 8 cut(s) 298, 904, 1134, 1804, 1969, 2053, 2295, 2359
BstFNI CGCG 2 cut(s) 590, 1288
BstH2I RGCGCY 1 cut(s) 1777
BstHHI GCGC 2 cut(s) 1290, 1776
BstMAI GTCTC 4 cut(s) 1110, 2123, 2142, 2489
BstMWI GCNNNNNNNGC 1 cut(s) 2447
BstNSI RCATGY 1 cut(s) 1226
BstSFI CTRYAG 2 cut(s) 616, 1338
BstSLI GKGCMC 1 cut(s) 2550
BstUI CGCG 2 cut(s) 590, 1288
BstV1I GCAGC 7 cut(s) 539, 602, 1856, 1879, 2437, 2440, 2550
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 1 cut(s) 36
BstXI CCANNNNNNTGG 1 cut(s) 2516
BstYI RGATCY 1 cut(s) 36
Bsu15I ATCGAT 1 cut(s) 759
BsuI GTATCC 1 cut(s) 688
BsuRI GGCC 7 cut(s) 252, 465, 705, 1233, 1870, 2111, 2308
BsuTUI ATCGAT 1 cut(s) 759
BtgI CCRYGG 2 cut(s) 1601, 2509
BtgZI GCGATG 1 cut(s) 125
BtrI CACGTC 2 cut(s) 1481, 1831
BtsCI GGATG 8 cut(s) 298, 904, 1134, 1804, 1969, 2053, 2295, 2359
BtsI GCAGTG 2 cut(s) 75, 1046
BtsIMutI CAGTG 7 cut(s) 75, 358, 834, 1046, 1148, 1649, 1864
Cac8I GCNNGC 6 cut(s) 201, 322, 441, 1245, 1372, 2113
CaiI CAGNNNCTG 2 cut(s) 1866, 2537
CfoI GCGC 2 cut(s) 1290, 1776
Cfr13I GGNCC 5 cut(s) 251, 704, 1541, 1598, 1869
ClaI ATCGAT 1 cut(s) 759
CseI GACGC 1 cut(s) 2203
CsiI ACCWGGT 1 cut(s) 1389
Csp6I GTAC 9 cut(s) 88, 129, 287, 512, 826, 1325, 1675, 1902, 2392
CviAII CATG 9 cut(s) 855, 981, 1223, 1602, 2149, 2282, 2321, 2510, 2587
CviQI GTAC 9 cut(s) 88, 129, 287, 512, 826, 1325, 1675, 1902, 2392
DdeI CTNAG 9 cut(s) 515, 774, 794, 816, 842, 1151, 1572, 1623, 1733
DrdI GACNNNNNNGTC 1 cut(s) 2240
DriI GACNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 2240
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 3 cut(s) 48, 261, 362
Eam1105I GACNNNNNGTC 1 cut(s) 161
EarI CTCTTC 3 cut(s) 48, 261, 362
Eco130I CCWWGG 4 cut(s) 578, 649, 1601, 2509
Eco147I AGGCCT 1 cut(s) 2111
Eco31I GGTCTC 1 cut(s) 2142
Eco32I GATATC 1 cut(s) 508
Eco47I GGWCC 2 cut(s) 1541, 1598
Eco57I CTGAAG 1 cut(s) 1992
Eco88I CYCGRG 1 cut(s) 2549
EcoRI GAATTC 2 cut(s) 1582, 1723
EcoRV GATATC 1 cut(s) 508
EcoT14I CCWWGG 4 cut(s) 578, 649, 1601, 2509
EcoT22I ATGCAT 1 cut(s) 986
ErhI CCWWGG 4 cut(s) 578, 649, 1601, 2509
FaeI CATG 9 cut(s) 858, 984, 1226, 1605, 2152, 2285, 2324, 2513, 2590
FaqI GGGAC 1 cut(s) 2311
FatI CATG 9 cut(s) 854, 980, 1222, 1601, 2148, 2281, 2320, 2509, 2586
FauI CCCGC 1 cut(s) 954
FbaI TGATCA 3 cut(s) 411, 1669, 2577
FblI GTMKAC 1 cut(s) 1992
Fnu4HI GCNGC 7 cut(s) 528, 616, 1845, 1893, 2451, 2454, 2564
FokI GGATG 8 cut(s) 305, 911, 1141, 1811, 1976, 2060, 2302, 2366
Fsp4HI GCNGC 7 cut(s) 528, 616, 1845, 1893, 2451, 2454, 2564
FspBI CTAG 4 cut(s) 713, 1094, 1158, 2447
GlaI GCGC 2 cut(s) 1289, 1775
GluI GCNGC 7 cut(s) 528, 616, 1845, 1893, 2451, 2454, 2564
GsaI CCCAGC 1 cut(s) 2289
GsuI CTGGAG 1 cut(s) 1215
HaeII RGCGCY 1 cut(s) 1777
HaeIII GGCC 7 cut(s) 252, 465, 705, 1233, 1870, 2111, 2308
HapII CCGG 3 cut(s) 2024, 2219, 2543
HgaI GACGC 1 cut(s) 2203
HhaI GCGC 2 cut(s) 1290, 1776
Hin1II CATG 9 cut(s) 858, 984, 1226, 1605, 2152, 2285, 2324, 2513, 2590
Hin6I GCGC 2 cut(s) 1288, 1774
HinP1I GCGC 2 cut(s) 1288, 1774
HincII GTYRAC 1 cut(s) 1213
HindII GTYRAC 1 cut(s) 1213
HindIII AAGCTT 1 cut(s) 235
HinfI GANTC 7 cut(s) 470, 965, 1615, 1786, 2060, 2170, 2520
HpaII CCGG 3 cut(s) 2024, 2219, 2543
HphI GGTGA 8 cut(s) 505, 914, 1675, 1806, 1864, 1902, 2193, 2240
Hpy166II GTNNAC 7 cut(s) 73, 243, 279, 1213, 1484, 1993, 2392
Hpy8I GTNNAC 7 cut(s) 73, 243, 279, 1213, 1484, 1993, 2392
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 4 cut(s) 763, 1223, 1761, 1845
HpyCH4IV ACGT 5 cut(s) 460, 675, 738, 1480, 1830
HpyF10VI GCNNNNNNNGC 1 cut(s) 2447
HpyF3I CTNAG 9 cut(s) 515, 774, 794, 816, 842, 1151, 1572, 1623, 1733
HpySE526I ACGT 5 cut(s) 460, 675, 738, 1480, 1830
Hsp92II CATG 9 cut(s) 858, 984, 1226, 1605, 2152, 2285, 2324, 2513, 2590
HspAI GCGC 2 cut(s) 1288, 1774
KpnI GGTACC 1 cut(s) 132
Ksp22I TGATCA 3 cut(s) 411, 1669, 2577
LmnI GCTCC 7 cut(s) 196, 341, 1252, 1495, 1657, 1741, 2431
Lsp1109I GCAGC 7 cut(s) 539, 602, 1856, 1879, 2437, 2440, 2550
LweI GCATC 5 cut(s) 714, 993, 1135, 1159, 1170
MabI ACCWGGT 1 cut(s) 1389
MaeI CTAG 4 cut(s) 713, 1094, 1158, 2447
MaeII ACGT 5 cut(s) 460, 675, 738, 1480, 1830
MaeIII GTNAC 8 cut(s) 1333, 1376, 1466, 1636, 1803, 1883, 2246, 2553
MflI RGATCY 1 cut(s) 36
MhlI GDGCHC 2 cut(s) 2279, 2550
MlsI TGGCCA 1 cut(s) 465
MluNI TGGCCA 1 cut(s) 465
MmeI TCCRAC 3 cut(s) 363, 577, 2472
Mox20I TGGCCA 1 cut(s) 465
Mph1103I ATGCAT 1 cut(s) 986
MroXI GAANNNNTTC 3 cut(s) 480, 1723, 2370
MscI TGGCCA 1 cut(s) 465
MseI TTAA 7 cut(s) 375, 401, 666, 741, 1070, 1308, 2599
MslI CAYNNNNRTG 5 cut(s) 686, 1479, 2319, 2514, 2571
Msp20I TGGCCA 1 cut(s) 465
MspA1I CMGCKG 1 cut(s) 1847
MspI CCGG 3 cut(s) 2024, 2219, 2543
Mva1269I GAATGC 5 cut(s) 457, 529, 786, 1744, 2197
MvnI CGCG 2 cut(s) 590, 1288
MwoI GCNNNNNNNGC 1 cut(s) 2447
NciI CCSGG 1 cut(s) 2220
NcoI CCATGG 2 cut(s) 1601, 2509
NlaIII CATG 9 cut(s) 858, 984, 1226, 1605, 2152, 2285, 2324, 2513, 2590
NlaIV GGNNCC 7 cut(s) 130, 337, 706, 1388, 1443, 2224, 2547
NmeAIII GCCGAG 2 cut(s) 1087, 2284
NmuCI GTSAC 4 cut(s) 1333, 1376, 1803, 2246
NsiI ATGCAT 1 cut(s) 986
NspI RCATGY 1 cut(s) 1226
OliI CACNNNNGTG 1 cut(s) 1479
PceI AGGCCT 1 cut(s) 2111
PctI GAATGC 5 cut(s) 457, 529, 786, 1744, 2197
PdmI GAANNNNTTC 3 cut(s) 480, 1723, 2370
PfeI GAWTC 7 cut(s) 470, 965, 1615, 1786, 2060, 2170, 2520
PflFI GACNNNGTC 1 cut(s) 2134
PflMI CCANNNNNTGG 2 cut(s) 332, 1866
PfoI TCCNGGA 1 cut(s) 1537
PkrI GCNGC 7 cut(s) 529, 617, 1846, 1894, 2452, 2455, 2565
Ppu21I YACGTR 1 cut(s) 461
Psp1406I AACGTT 1 cut(s) 738
PspFI CCCAGC 1 cut(s) 2285
PspN4I GGNNCC 7 cut(s) 130, 337, 706, 1388, 1443, 2224, 2547
PspPI GGNCC 5 cut(s) 251, 704, 1541, 1598, 1869
PstI CTGCAG 1 cut(s) 620
PstNI CAGNNNCTG 2 cut(s) 1866, 2537
PsuI RGATCY 1 cut(s) 36
PsyI GACNNNGTC 1 cut(s) 2134
RsaI GTAC 9 cut(s) 89, 130, 288, 513, 827, 1326, 1676, 1903, 2393
RsaNI GTAC 9 cut(s) 88, 129, 287, 512, 826, 1325, 1675, 1902, 2392
RseI CAYNNNNRTG 5 cut(s) 686, 1479, 2319, 2514, 2571
SaqAI TTAA 7 cut(s) 375, 401, 666, 741, 1070, 1308, 2599
SatI GCNGC 7 cut(s) 528, 616, 1845, 1893, 2451, 2454, 2564
Sau96I GGNCC 5 cut(s) 251, 704, 1541, 1598, 1869
ScaI AGTACT 1 cut(s) 89
SduI GDGCHC 2 cut(s) 2279, 2550
SexAI ACCWGGT 1 cut(s) 1389
SfaNI GCATC 5 cut(s) 714, 993, 1135, 1159, 1170
SfcI CTRYAG 2 cut(s) 616, 1338
SinI GGWCC 2 cut(s) 1541, 1598
SmiMI CAYNNNNRTG 5 cut(s) 686, 1479, 2319, 2514, 2571
SmlI CTYRAG 3 cut(s) 272, 1025, 2503
SmoI CTYRAG 3 cut(s) 272, 1025, 2503
SpeI ACTAGT 2 cut(s) 1093, 1157
SseBI AGGCCT 1 cut(s) 2111
SsiI CCGC 7 cut(s) 491, 590, 961, 1286, 1413, 1822, 1847
SspI AATATT 1 cut(s) 932
SspMI CTAG 4 cut(s) 713, 1094, 1158, 2447
StuI AGGCCT 1 cut(s) 2111
StyI CCWWGG 4 cut(s) 578, 649, 1601, 2509
TaiI ACGT 5 cut(s) 463, 678, 741, 1483, 1833
TaqI TCGA 4 cut(s) 138, 305, 759, 884
TatI WGTACW 4 cut(s) 87, 286, 825, 1674
TfiI GAWTC 7 cut(s) 470, 965, 1615, 1786, 2060, 2170, 2520
Tru1I TTAA 7 cut(s) 375, 401, 666, 741, 1070, 1308, 2599
Tru9I TTAA 7 cut(s) 375, 401, 666, 741, 1070, 1308, 2599
TscAI CASTG 7 cut(s) 75, 358, 841, 1053, 1155, 1649, 1871
TseFI GTSAC 4 cut(s) 1333, 1376, 1803, 2246
TseI GCWGC 7 cut(s) 527, 615, 1844, 1892, 2450, 2453, 2563
Tsp45I GTSAC 4 cut(s) 1333, 1376, 1803, 2246
TspGWI ACGGA 4 cut(s) 240, 1284, 2366, 2407
TspRI CASTG 7 cut(s) 75, 358, 841, 1053, 1155, 1649, 1871
Tth111I GACNNNGTC 1 cut(s) 2134
Van91I CCANNNNNTGG 2 cut(s) 332, 1866
VpaK11BI GGWCC 2 cut(s) 1541, 1598
XapI RAATTY 5 cut(s) 212, 1303, 1582, 1723, 2117
XbaI TCTAGA 1 cut(s) 712
XceI RCATGY 1 cut(s) 1226
XmiI GTMKAC 1 cut(s) 1992
XmnI GAANNNNTTC 3 cut(s) 480, 1723, 2370
XspI CTAG 4 cut(s) 713, 1094, 1158, 2447
ZrmI AGTACT 1 cut(s) 89
Zsp2I ATGCAT 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.