Rroxscaffold_7G00201260

Argonaute

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001623
Physical Location & Seq
Forward (+)
48851652 .. 48852800
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00201260.1

Sequence Viewer

Length: 366 bp
ATGGCCTCCAAGAGCATCTTGAAGGAGCTTAAGGATTTGCGAAGGATCCTCCCACTTCCTTCAGCACAGATTGCCATGCAATATGATGATGGAACCGAAGTTGACGGGAAAGGCATTAGACGTAAGGTTCTTGAAAAAGTAAAAGACACATATAGTGTGGGGCTGGAAAACAAGGATTTTGCTTATGATGGAGAGAAGAGCTTGTTTCTTTCTGTTGGGCAGCTCCCTCATAAAAAACTTGATTTCACGGTAGTACCGGATGACATATCATCAAGCAGATGTGTCAACTACAATGATTGTAAAACCTGGTCCTGTTTGGAATTTCCTCATGGAGAACCAGAATGTCAAAACTCCTTACCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

121

Amino Acids

13.65

Weight (kDa)

5.4

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 23 - 88 3.8e-06 N-terminal domain of argonaute
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 40, 53
AcsI RAATTY 1 cut(s) 320
AcuI CTGAAG 1 cut(s) 45
AfaI GTAC 1 cut(s) 255
AflII CTTAAG 1 cut(s) 29
AgsI TTSAA 2 cut(s) 22, 134
AjnI CCWGG 1 cut(s) 305
AluBI AGCT 3 cut(s) 28, 201, 223
AluI AGCT 3 cut(s) 28, 201, 223
AlwI GGATC 2 cut(s) 40, 53
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 220
ApoI RAATTY 1 cut(s) 320
AspS9I GGNCC 1 cut(s) 309
AvaII GGWCC 1 cut(s) 309
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BamHI GGATCC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 232
BccI CCATC 2 cut(s) 83, 182
BciT130I CCWGG 1 cut(s) 307
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 1 cut(s) 221
BlsI GCNGC 1 cut(s) 222
Bme1390I CCNGG 1 cut(s) 307
Bme18I GGWCC 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 309
BmiI GGNNCC 2 cut(s) 47, 94
BmrFI CCNGG 1 cut(s) 307
BmsI GCATC 1 cut(s) 24
BsaWI WCCGGW 2 cut(s) 256, 358
BseBI CCWGG 1 cut(s) 307
BseGI GGATG 1 cut(s) 265
BseXI GCAGC 1 cut(s) 232
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 2 cut(s) 257, 359
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 45
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 2 cut(s) 47, 94
BspPI GGATC 2 cut(s) 40, 53
BspQI GCTCTTC 1 cut(s) 191
BspTI CTTAAG 1 cut(s) 29
BssMI GATC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 307
Bst4CI ACNGT 1 cut(s) 250
Bst6I CTCTTC 1 cut(s) 191
BstAFI CTTAAG 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 71
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 1 cut(s) 48
BstMBI GATC 1 cut(s) 45
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 307
BstSCI CCNGG 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 45
BstYI RGATCY 1 cut(s) 45
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 265
Cfr13I GGNCC 1 cut(s) 309
CsiI ACCWGGT 1 cut(s) 305
Csp6I GTAC 1 cut(s) 254
CviAII CATG 2 cut(s) 76, 329
CviJI RGCY 5 cut(s) 5, 28, 163, 201, 223
CviKI_1 RGCY 5 cut(s) 5, 28, 163, 201, 223
CviQI GTAC 1 cut(s) 254
DpnI GATC 1 cut(s) 47
DpnII GATC 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 305
FaeI CATG 2 cut(s) 79, 332
FaiI YATR 8 cut(s) 77, 84, 151, 153, 186, 231, 266, 330
FalI AAGNNNNNCTT 1 cut(s) 34
FatI CATG 2 cut(s) 75, 328
Fnu4HI GCNGC 1 cut(s) 221
FokI GGATG 1 cut(s) 272
Fsp4HI GCNGC 1 cut(s) 221
GluI GCNGC 1 cut(s) 221
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 257, 359
Hin1II CATG 2 cut(s) 79, 332
HincII GTYRAC 2 cut(s) 103, 286
HindII GTYRAC 2 cut(s) 103, 286
HpaII CCGG 2 cut(s) 257, 359
Hpy166II GTNNAC 2 cut(s) 103, 286
Hpy188III TCNNGA 2 cut(s) 19, 131
Hpy8I GTNNAC 2 cut(s) 103, 286
HpyAV CCTTC 3 cut(s) 16, 36, 69
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 121
HpyCH4V TGCA 1 cut(s) 79
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 121
Hsp92II CATG 2 cut(s) 79, 332
Kzo9I GATC 1 cut(s) 45
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 2 cut(s) 25, 228
LpnPI CCDG 6 cut(s) 149, 270, 292, 319, 325, 351
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 1 cut(s) 24
MabI ACCWGGT 1 cut(s) 305
MaeII ACGT 1 cut(s) 121
MalI GATC 1 cut(s) 47
MboI GATC 1 cut(s) 45
MboII GAAGA 1 cut(s) 208
MflI RGATCY 1 cut(s) 45
MluCI AATT 1 cut(s) 320
MnlI CCTC 4 cut(s) 16, 59, 237, 336
MseI TTAA 1 cut(s) 30
MspCI CTTAAG 1 cut(s) 29
MspI CCGG 2 cut(s) 257, 359
MspR9I CCNGG 1 cut(s) 307
MvaI CCWGG 1 cut(s) 307
MwoI GCNNNNNNNGC 1 cut(s) 71
NdeII GATC 1 cut(s) 45
NlaIII CATG 2 cut(s) 79, 332
NlaIV GGNNCC 2 cut(s) 47, 94
PciSI GCTCTTC 1 cut(s) 191
PkrI GCNGC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 305
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 2 cut(s) 47, 94
PspPI GGNCC 1 cut(s) 309
PsuI RGATCY 1 cut(s) 45
RsaI GTAC 1 cut(s) 255
RsaNI GTAC 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 1 cut(s) 221
Sau3AI GATC 1 cut(s) 45
Sau96I GGNCC 1 cut(s) 309
ScrFI CCNGG 1 cut(s) 307
SetI ASST 6 cut(s) 30, 124, 129, 203, 225, 308
SexAI ACCWGGT 1 cut(s) 305
SfaNI GCATC 1 cut(s) 24
SinI GGWCC 1 cut(s) 309
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 1 cut(s) 320
StyD4I CCNGG 1 cut(s) 305
TaaI ACNGT 1 cut(s) 250
TaiI ACGT 1 cut(s) 124
TasI AATT 1 cut(s) 320
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TseI GCWGC 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 309
XapI RAATTY 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.