MD07G1052400.v1.1

gene silencing by RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001623
Physical Location & Seq
Forward (+)
4849015 .. 4854762
5748 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1052400.v1.1.491

Sequence Viewer

Length: 2892 bp
ATGTGTATTCCTCCTGATGAGTTAGTGGCAGGACAATTGTTTTGGAAGAGAAACTGGCTCAAGTTTGAATTTGGTAGAGATGTATCTCTTTGTTACCTGCAGTTTACACCTACATTGCTTATCAATTGCGGGGTTGGCAGGTATAAGGTAATGGAGTCTTCCGATCCCAAAGGAGCTGAGCTTCATCCTCCTCCCGCCATTATTCCTCCAAAAGTTGTCCCAACTGTGGCAGAAGGAAAATCTGTTGAAGTGTTTAAGAAACCATCGACTTCTAAACTTGTTCCCATGTCAAGGCCAGGCTTTGGATCTAGAGGAAAAAACATCCAACTAACAACTAACCATTTCAAAGTGGGAGTGAAAATTTCGGATGGCTACTTTTTCCATTATAGTATTGACATGTTTTATGAGGATGGAACCCCAGTTACAGCGAACGCTATTGGACGCAAAGTTGTTGAAAAAGTTAAAGAGACATATGACAGGGATCTAGGACGAAAAGAATTTGCTTATGATGGACAGAAGAACTTGTTTACTGTTGGCTCTCTCCCTCATAAAAGACTCGACTTTGTGGTAGTATTGGACAACATCTCGTCAAGCAGGCGAATTGGGAATGTTGGAGGTAGTAGTAGTTACGACCCAGCTGAGAGTGGAAGCGATAGGAAGAGACTTAAGCATTCATTTCAATCCAAAACAATAAAGGTGCTGATAAATTATGCTACTAAAATCCCAATGCAAGCGATCGTAAATGGATCACGAGGTCAGGATTCAAAGGAGTTTCAGGAAGCAGTTAGAGTTCTGGACATCATTCTAAGGCAGCATGCAGTAAAGCAGGGTTGTCTCCTTGTCCGACAGTCTTTCTTCCGTAACAATCCAAGGAATCCAGACTTGGGAGAAGGCTTGTCAGGCTGCTCGGGCTTCCATTCAAGTTTCCGAGCTACCCAAGCGGGTCTTTCCCTGAACATGGATGTATCCACTACAGTGATCATAAAACCAGGTCCCGTTTTGAACTTCCTCTTGGAGAACCAAAAAGTTAATCACCTTCGCCATATTGATTGGATCAAGGCTAAAAGGACGCTAAAAAATCTACGGATCAGGATCGAATCCTCTGGTATGGAGTACAAAATCACTGGATTGAGTGACGATTCCTGCAAAGAGCAGACGTTTTTTCTAAAAAAGAGTAAAGGACAAGATGGTGACGGAGAAGAAGTTACTGTTTATGACTACTTTGTTCGCCATAAACACATACCTGTACGTGAGTCAGCAGATTTTCCATGCATCAATGTTGGGAAAGCAAAGCGGCCATCTTTCTTTCCACTTGAGCTTTGTACCTTGGTTCCATTGCAACGCTATACCAAAGCCTTGTCCACTAAGCAAAGGTCTACACTAGTGGGGGAGTCCCGGCAGAAGCCCCAGGAGCGGATTTCAGTTTTGCAACGTGAGCTGAGAAGTAGTAACTACAATGCTGACCGGATGCTTCAATCTTCTGGAATATCAATCAGTCCTGAATTTATGCAGGTTGAAGGCCGTGTGCTGTCAGCCCCAAGGTTAAAAGTGGGCAATGGGAATGATTTTTTCCCCCGGAATGGGCGCTGGAATTTCAGCAATATGAGTTTTGTGGAACCGGTAAAAATTGAGACGTGGGCAATTGTCAACTTCTCTGATTTCCGTGACATTGATTTCCTGATCAAAACTATGTTGAAGTGTGGAGATATGAGAGGAATTGCCATACATCCTCCAAGTCATGTATTTCAAGAGAAAAATCAGCATAAGCGCGAGCCAGCTGCGGTCCGAGTGGACAAGGTGTTTGAAGAAAGAGGATTGAGAATTAAGAAAGAAATACCAAAGCTTCTGCTGTGTATTCTTCCTGAGAGGAATAACATATATGGTCCATGGAAAAGGAAATGTCTCTCCGAGGTCGGGGTTATAACACAGTGCATTATCTGGAAGGGTAAAAGGATGAATGATCAGTATATCACAAATGTGCTCCTCAAAATCAATGCTAAGCTTGGTGGAATGAACACCTATCTAACAGCGGAGTATTCTCGTTCTATACCTATGGCTTCCAGGTCTCCTACTATGATTTTGGGTATGGATGTGTCCCATGGCTCACCTGGGCGGTCAGATGTTCCTTCTATTGCAGTGGTGGTTGGTTCGAGGAAATGGCCCTCAATTTCTCATTATCGCGCCTCAGTTCGTACTCAATCACCGAAAGTAGAAATGATTGCTTCTCTGTTCAAGCCTGTGTCAGAAAAAGATGATGAGGGCATAATCAGGGAACTGCTACTAGACTTTTATCGTTCATCAAACAGCTGTAAGCCGGCTCAGATAATAATTTTCAGGGATGGAACGAGCGAATCACAGTTCGACCAAGTCTTGAATGATGAAATGAGCCAGATCATTCAGGCCTGCAAGTTTCTTGATGAGGGGTGGTCTCCAAAGTTCATGGTAATTGTAGCACAAAAGAATCACCATACAAAATTCTTCCAGACCGGTTCTCCTCATAATGTTCCACCGGGGACTATCATTGACAGAACAGTTTGTCATCCAACTAACAATGATTTCTACCTGTGTGCTCATGCTGGGATGATTGGGACTACACGACCTACTCATTATCATGTTCTACTTGATCAACTTGGCTATTCAGCAGATGACTTGCAAGAACTTGTTCACTCTTTATCGTATGTGTTCCAAAGGAGTACCACAGCCATATCAGTAGTTGCTCCGATACGCTATGCTCATCTGGCAGCCGCTCAGATGTCGCAGTTCATTGACTTCGATGAAATGTCGGAGACTTCCTCAAGCCGAGGTGGTAGTGTTACAGTAGCTGGTGGTGTGGCGGTGCCTGAATTACCAAAGCTGCATGAAGATGTCAAGAACTACATGTTCTTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

964

Amino Acids

108.46

Weight (kDa)

9.37

Isoelectric Point (pI)

45.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 106 - 269 2.9e-24 N-terminal domain of argonaute
ArgoL1 PF08699 281 - 329 5.2e-15 Argonaute linker 1 domain
PAZ PF02170 335 - 461 2.3e-24 PAZ domain
ArgoL2 PF16488 472 - 516 4.2e-10 Argonaute linker 2 domain
ArgoMid PF16487 525 - 575 1.4e-06 Mid domain of argonaute
Piwi PF02171 616 - 922 1.2e-100 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1922
Acc36I ACCTGC 3 cut(s) 105, 129, 1501
AccB1I GGYRCC 1 cut(s) 2836
AccB7I CCANNNNNTGG 1 cut(s) 302
AccBSI CCGCTC 2 cut(s) 1414, 2747
AccI GTMKAC 1 cut(s) 1376
AccII CGCG 2 cut(s) 1770, 2181
AclWI GGATC 7 cut(s) 158, 313, 489, 754, 1061, 1094, 1100
AcoI YGGCCR 1 cut(s) 1295
AcsI RAATTY 6 cut(s) 68, 360, 497, 1502, 1591, 2474
AfaI GTAC 5 cut(s) 1115, 1248, 1324, 2194, 2695
AfiI CCNNNNNNNGG 9 cut(s) 226, 291, 302, 884, 958, 1413, 1580, 1581, 1914
AflII CTTAAG 1 cut(s) 665
AflIII ACRYGT 2 cut(s) 396, 2877
AgeI ACCGGT 2 cut(s) 1618, 2486
AhlI ACTAGT 1 cut(s) 1381
AjiI CACGTC 1 cut(s) 1635
AjnI CCWGG 5 cut(s) 295, 988, 1407, 2060, 2107
AjuI GAANNNNNNNTTGG 4 cut(s) 866, 898, 995, 1027
AleI CACNNNNGTG 2 cut(s) 974, 1976
Alw21I GWGCWC 2 cut(s) 1983, 2572
Alw26I GTCTC 8 cut(s) 461, 655, 839, 1625, 1907, 2070, 2433, 2780
AlwI GGATC 7 cut(s) 158, 313, 489, 754, 1061, 1094, 1100
AlwNI CAGNNNCTG 1 cut(s) 2822
Ama87I CYCGRG 1 cut(s) 907
AoxI GGCC 5 cut(s) 293, 1295, 1519, 2159, 2400
ApeKI GCWGC 5 cut(s) 811, 903, 1778, 2741, 2854
ApoI RAATTY 6 cut(s) 68, 360, 497, 1502, 1591, 2474
ArsI GACNNNNNNTTYG 4 cut(s) 24, 56, 1784, 1816
AsiGI ACCGGT 2 cut(s) 1618, 2486
Asp700I GAANNNNTTC 1 cut(s) 2661
AspLEI GCGC 3 cut(s) 1587, 1770, 2183
AspS9I GGNCC 4 cut(s) 992, 1783, 1883, 2160
AsuC2I CCSGG 3 cut(s) 1396, 1576, 2511
AsuHPI GGTGA 5 cut(s) 1025, 1202, 2097, 2193, 2456
AvaI CYCGRG 1 cut(s) 907
AvaII GGWCC 3 cut(s) 992, 1783, 1883
BanI GGYRCC 1 cut(s) 2836
BauI CACGAG 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 2 cut(s) 1983, 2572
BbvI GCAGC 5 cut(s) 823, 890, 1765, 2753, 2841
BccI CCATC 7 cut(s) 271, 362, 404, 503, 1181, 1306, 2333
BceAI ACGGC 1 cut(s) 1506
BcgI CGANNNNNNTGC 2 cut(s) 1760, 1794
BciT130I CCWGG 5 cut(s) 297, 990, 1409, 2062, 2109
BciVI GTATCC 1 cut(s) 976
BclI TGATCA 4 cut(s) 978, 1680, 1960, 2623
BcnI CCSGG 3 cut(s) 1396, 1576, 2511
BcoDI GTCTC 8 cut(s) 461, 655, 839, 1625, 1907, 2070, 2433, 2780
BcuI ACTAGT 1 cut(s) 1381
BfaI CTAG 4 cut(s) 309, 485, 1382, 2282
BfmI CTRYAG 2 cut(s) 98, 972
BfoI RGCGCY 1 cut(s) 1588
BfrI CTTAAG 1 cut(s) 665
BfuAI ACCTGC 3 cut(s) 105, 129, 1501
BfuI GTATCC 1 cut(s) 976
BisI GCNGC 7 cut(s) 812, 904, 1295, 1779, 2742, 2745, 2855
BlpI GCTNAGC 2 cut(s) 177, 1998
BlsI GCNGC 7 cut(s) 813, 905, 1296, 1780, 2743, 2746, 2856
Bme1390I CCNGG 8 cut(s) 297, 990, 1396, 1409, 1576, 2062, 2109, 2511
Bme18I GGWCC 3 cut(s) 992, 1783, 1883
BmeT110I CYCGRG 1 cut(s) 907
BmgBI CACGTC 1 cut(s) 1635
BmgT120I GGNCC 4 cut(s) 992, 1783, 1883, 2160
BmiI GGNNCC 5 cut(s) 415, 994, 1332, 1617, 2838
BmrFI CCNGG 8 cut(s) 297, 990, 1396, 1409, 1576, 2062, 2109, 2511
BmrI ACTGGG 1 cut(s) 413
BmsI GCATC 2 cut(s) 1281, 1458
BmuI ACTGGG 1 cut(s) 413
BpiI GAAGAC 1 cut(s) 150
BplI GAGNNNNNCTC 2 cut(s) 2777, 2809
Bpu1102I GCTNAGC 2 cut(s) 177, 1998
BpuEI CTTGAG 3 cut(s) 44, 1334, 2779
BpuMI CCSGG 3 cut(s) 1396, 1576, 2511
BsaAI YACGTR 1 cut(s) 1250
BsaBI GATNNNNATC 1 cut(s) 1091
BsaI GGTCTC 2 cut(s) 2070, 2433
BsaWI WCCGGW 3 cut(s) 1464, 1618, 2486
BsaXI ACNNNNNCTCC 4 cut(s) 1188, 1218, 2476, 2506
Bsc4I CCNNNNNNNGG 9 cut(s) 226, 291, 302, 884, 958, 1413, 1580, 1581, 1914
Bse118I RCCGGY 3 cut(s) 1618, 2314, 2486
Bse1I ACTGG 3 cut(s) 59, 419, 1129
Bse3DI GCAATG 3 cut(s) 113, 1334, 1561
Bse8I GATNNNNATC 1 cut(s) 1091
BseBI CCWGG 5 cut(s) 297, 990, 1409, 2062, 2109
BseJI GATNNNNATC 1 cut(s) 1091
BseLI CCNNNNNNNGG 9 cut(s) 226, 291, 302, 884, 958, 1413, 1580, 1581, 1914
BseMI GCAATG 3 cut(s) 113, 1334, 1561
BseMII CTCAG 7 cut(s) 168, 630, 1430, 1854, 2199, 2333, 2762
BseNI ACTGG 3 cut(s) 59, 419, 1129
BseRI GAGGAG 3 cut(s) 180, 1973, 2484
BseXI GCAGC 5 cut(s) 823, 890, 1765, 2753, 2841
BseYI CCCAGC 2 cut(s) 634, 2576
Bsh1236I CGCG 2 cut(s) 1770, 2181
Bsh1285I CGRYCG 1 cut(s) 738
BshFI GGCC 5 cut(s) 295, 1297, 1521, 2161, 2402
BshNI GGYRCC 1 cut(s) 2836
BshTI ACCGGT 2 cut(s) 1618, 2486
BsiEI CGRYCG 1 cut(s) 738
BsiHKAI GWGCWC 2 cut(s) 1983, 2572
BsiHKCI CYCGRG 1 cut(s) 907
BsiSI CCGG 7 cut(s) 1396, 1465, 1576, 1619, 2315, 2487, 2510
BslFI GGGAC 6 cut(s) 203, 978, 1378, 2080, 2527, 2602
BslI CCNNNNNNNGG 9 cut(s) 226, 291, 302, 884, 958, 1413, 1580, 1581, 1914
BsmAI GTCTC 8 cut(s) 461, 655, 839, 1625, 1907, 2070, 2433, 2780
BsmBI CGTCTC 1 cut(s) 1625
BsmFI GGGAC 6 cut(s) 203, 978, 1378, 2080, 2527, 2602
BsmI GAATGC 1 cut(s) 670
BsnI GGCC 5 cut(s) 295, 1297, 1521, 2161, 2402
Bso31I GGTCTC 2 cut(s) 2070, 2433
BsoBI CYCGRG 1 cut(s) 907
Bsp1286I GDGCHC 2 cut(s) 1983, 2572
Bsp1720I GCTNAGC 2 cut(s) 177, 1998
Bsp19I CCATGG 2 cut(s) 1886, 2098
BspANI GGCC 5 cut(s) 295, 1297, 1521, 2161, 2402
BspCNI CTCAG 7 cut(s) 169, 631, 1431, 1855, 2198, 2332, 2761
BspFNI CGCG 2 cut(s) 1770, 2181
BspLI GGNNCC 5 cut(s) 415, 994, 1332, 1617, 2838
BspMAI CTGCAG 1 cut(s) 102
BspMI ACCTGC 3 cut(s) 105, 129, 1501
BspPI GGATC 7 cut(s) 158, 313, 489, 754, 1061, 1094, 1100
BspT107I GGYRCC 1 cut(s) 2836
BspTI CTTAAG 1 cut(s) 665
BspTNI GGTCTC 2 cut(s) 2070, 2433
BsrBI CCGCTC 2 cut(s) 1414, 2747
BsrDI GCAATG 3 cut(s) 113, 1334, 1561
BsrFI RCCGGY 3 cut(s) 1618, 2314, 2486
BsrI ACTGG 3 cut(s) 59, 419, 1129
BssAI RCCGGY 3 cut(s) 1618, 2314, 2486
BssSI CACGAG 1 cut(s) 750
BssT1I CCWWGG 5 cut(s) 869, 1326, 1538, 1886, 2098
Bst2BI CACGAG 1 cut(s) 750
Bst2UI CCWGG 5 cut(s) 297, 990, 1409, 2062, 2109
Bst4CI ACNGT 9 cut(s) 226, 532, 849, 976, 1210, 1929, 2358, 2533, 2818
Bst6I CTCTTC 2 cut(s) 41, 653
BstAFI CTTAAG 1 cut(s) 665
BstBAI YACGTR 1 cut(s) 1250
BstC8I GCNNGC 7 cut(s) 596, 732, 816, 1772, 1776, 2316, 2404
BstDSI CCRYGG 2 cut(s) 1886, 2098
BstFNI CGCG 2 cut(s) 1770, 2181
BstH2I RGCGCY 1 cut(s) 1588
BstHHI GCGC 3 cut(s) 1587, 1770, 2183
BstMAI GTCTC 8 cut(s) 461, 655, 839, 1625, 1907, 2070, 2433, 2780
BstMCI CGRYCG 1 cut(s) 738
BstMWI GCNNNNNNNGC 7 cut(s) 135, 900, 909, 938, 1411, 1435, 2738
BstNI CCWGG 5 cut(s) 297, 990, 1409, 2062, 2109
BstNSI RCATGY 3 cut(s) 400, 818, 2881
BstSCI CCNGG 8 cut(s) 295, 988, 1394, 1407, 1574, 2060, 2107, 2509
BstSFI CTRYAG 2 cut(s) 98, 972
BstUI CGCG 2 cut(s) 1770, 2181
BstV1I GCAGC 5 cut(s) 823, 890, 1765, 2753, 2841
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 2 cut(s) 305, 481
BstYI RGATCY 2 cut(s) 305, 481
BsuI GTATCC 1 cut(s) 976
BsuRI GGCC 5 cut(s) 295, 1297, 1521, 2161, 2402
BtgI CCRYGG 2 cut(s) 1886, 2098
BtrI CACGTC 1 cut(s) 1635
BtsI GCAGTG 1 cut(s) 2142
BtsIMutI CAGTG 4 cut(s) 981, 1122, 1934, 2142
BveI ACCTGC 3 cut(s) 105, 129, 1501
Cac8I GCNNGC 7 cut(s) 596, 732, 816, 1772, 1776, 2316, 2404
CaiI CAGNNNCTG 1 cut(s) 2822
CfoI GCGC 3 cut(s) 1587, 1770, 2183
Cfr10I RCCGGY 3 cut(s) 1618, 2314, 2486
Cfr13I GGNCC 4 cut(s) 992, 1783, 1883, 2160
CpoI CGGWCCG 1 cut(s) 1783
CseI GACGC 2 cut(s) 450, 1078
CsiI ACCWGGT 1 cut(s) 988
Csp6I GTAC 5 cut(s) 1114, 1247, 1323, 2193, 2694
CspAI ACCGGT 2 cut(s) 1618, 2486
CspI CGGWCCG 1 cut(s) 1783
CviQI GTAC 5 cut(s) 1114, 1247, 1323, 2193, 2694
EaeI YGGCCR 1 cut(s) 1295
Eam1104I CTCTTC 2 cut(s) 41, 653
EarI CTCTTC 2 cut(s) 41, 653
Eco130I CCWWGG 5 cut(s) 869, 1326, 1538, 1886, 2098
Eco147I AGGCCT 1 cut(s) 2402
Eco31I GGTCTC 2 cut(s) 2070, 2433
Eco47I GGWCC 3 cut(s) 992, 1783, 1883
Eco88I CYCGRG 1 cut(s) 907
EcoO109I RGGNCCY 1 cut(s) 992
EcoRII CCWGG 5 cut(s) 295, 988, 1407, 2060, 2107
EcoT14I CCWWGG 5 cut(s) 869, 1326, 1538, 1886, 2098
EcoT22I ATGCAT 1 cut(s) 1274
ErhI CCWWGG 5 cut(s) 869, 1326, 1538, 1886, 2098
Esp3I CGTCTC 1 cut(s) 1625
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FaqI GGGAC 6 cut(s) 203, 978, 1378, 2080, 2527, 2602
FauI CCCGC 3 cut(s) 122, 202, 934
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 4 cut(s) 978, 1680, 1960, 2623
FblI GTMKAC 1 cut(s) 1376
Fnu4HI GCNGC 7 cut(s) 812, 904, 1295, 1779, 2742, 2745, 2855
Fsp4HI GCNGC 7 cut(s) 812, 904, 1295, 1779, 2742, 2745, 2855
FspBI CTAG 4 cut(s) 309, 485, 1382, 2282
GlaI GCGC 3 cut(s) 1586, 1769, 2182
GluI GCNGC 7 cut(s) 812, 904, 1295, 1779, 2742, 2745, 2855
GsaI CCCAGC 2 cut(s) 638, 2580
HaeII RGCGCY 1 cut(s) 1588
HaeIII GGCC 5 cut(s) 295, 1297, 1521, 2161, 2402
HapII CCGG 7 cut(s) 1396, 1465, 1576, 1619, 2315, 2487, 2510
HgaI GACGC 2 cut(s) 450, 1078
HhaI GCGC 3 cut(s) 1587, 1770, 2183
Hin6I GCGC 3 cut(s) 1585, 1768, 2181
HinP1I GCGC 3 cut(s) 1585, 1768, 2181
HincII GTYRAC 1 cut(s) 1648
HindII GTYRAC 1 cut(s) 1648
HindIII AAGCTT 2 cut(s) 1841, 2000
HpaII CCGG 7 cut(s) 1396, 1465, 1576, 1619, 2315, 2487, 2510
HphI GGTGA 5 cut(s) 1025, 1202, 2097, 2193, 2456
Hpy166II GTNNAC 7 cut(s) 105, 528, 1362, 1377, 1648, 1792, 2665
Hpy8I GTNNAC 7 cut(s) 105, 528, 1362, 1377, 1648, 1792, 2665
HpyAV CCTTC 6 cut(s) 227, 884, 1046, 1511, 1936, 2136
HpyCH4III ACNGT 9 cut(s) 226, 532, 849, 976, 1210, 1929, 2358, 2533, 2818
HpyCH4IV ACGT 4 cut(s) 1157, 1249, 1432, 1634
HpyF10VI GCNNNNNNNGC 7 cut(s) 135, 900, 909, 938, 1411, 1435, 2738
HpySE526I ACGT 4 cut(s) 1157, 1249, 1432, 1634
HspAI GCGC 3 cut(s) 1585, 1768, 2181
KroI GCCGGC 1 cut(s) 2314
KroNI GCCGGC 1 cut(s) 2316
Ksp22I TGATCA 4 cut(s) 978, 1680, 1960, 2623
LmnI GCTCC 4 cut(s) 173, 1411, 1986, 2722
Lsp1109I GCAGC 5 cut(s) 823, 890, 1765, 2753, 2841
LweI GCATC 2 cut(s) 1281, 1458
MabI ACCWGGT 1 cut(s) 988
MaeI CTAG 4 cut(s) 309, 485, 1382, 2282
MaeII ACGT 4 cut(s) 1157, 1249, 1432, 1634
MbiI CCGCTC 2 cut(s) 1414, 2747
MfeI CAATTG 3 cut(s) 35, 124, 1641
MflI RGATCY 2 cut(s) 305, 481
MhlI GDGCHC 2 cut(s) 1983, 2572
MlyI GAGTC 4 cut(s) 164, 549, 1262, 1400
MmeI TCCRAC 5 cut(s) 349, 592, 868, 2567, 2763
Mph1103I ATGCAT 1 cut(s) 1274
MroNI GCCGGC 1 cut(s) 2314
MroXI GAANNNNTTC 1 cut(s) 2661
MseI TTAA 6 cut(s) 255, 462, 666, 1029, 1544, 1824
MslI CAYNNNNRTG 4 cut(s) 974, 1976, 2610, 2862
MspA1I CMGCKG 4 cut(s) 638, 1778, 2030, 2307
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 7 cut(s) 1396, 1465, 1576, 1619, 2315, 2487, 2510
MspR9I CCNGG 8 cut(s) 297, 990, 1396, 1409, 1576, 2062, 2109, 2511
MunI CAATTG 3 cut(s) 35, 124, 1641
Mva1269I GAATGC 1 cut(s) 670
MvaI CCWGG 5 cut(s) 297, 990, 1409, 2062, 2109
MvnI CGCG 2 cut(s) 1770, 2181
MwoI GCNNNNNNNGC 7 cut(s) 135, 900, 909, 938, 1411, 1435, 2738
NaeI GCCGGC 1 cut(s) 2316
NciI CCSGG 3 cut(s) 1396, 1576, 2511
NcoI CCATGG 2 cut(s) 1886, 2098
NdeI CATATG 1 cut(s) 472
NgoMIV GCCGGC 1 cut(s) 2314
NlaIV GGNNCC 5 cut(s) 415, 994, 1332, 1617, 2838
NmeAIII GCCGAG 1 cut(s) 2825
NmuCI GTSAC 3 cut(s) 1133, 1190, 1664
NsiI ATGCAT 1 cut(s) 1274
NspI RCATGY 3 cut(s) 400, 818, 2881
OliI CACNNNNGTG 2 cut(s) 974, 1976
PaeI GCATGC 1 cut(s) 818
PceI AGGCCT 1 cut(s) 2402
PciI ACATGT 2 cut(s) 396, 2877
PctI GAATGC 1 cut(s) 670
PdiI GCCGGC 1 cut(s) 2316
PdmI GAANNNNTTC 1 cut(s) 2661
PfeI GAWTC 6 cut(s) 761, 874, 1097, 1139, 2351, 2461
PflFI GACNNNGTC 1 cut(s) 2366
PflMI CCANNNNNTGG 1 cut(s) 302
PinAI ACCGGT 2 cut(s) 1618, 2486
PkrI GCNGC 7 cut(s) 813, 905, 1296, 1780, 2743, 2746, 2856
Ple19I CGATCG 1 cut(s) 738
PleI GAGTC 4 cut(s) 163, 549, 1261, 1399
PpsI GAGTC 4 cut(s) 163, 549, 1261, 1399
Ppu21I YACGTR 1 cut(s) 1250
PpuMI RGGWCCY 1 cut(s) 992
PscI ACATGT 2 cut(s) 396, 2877
PsiI TTATAA 1 cut(s) 1922
Psp5II RGGWCCY 1 cut(s) 992
Psp6I CCWGG 5 cut(s) 295, 988, 1407, 2060, 2107
PspFI CCCAGC 2 cut(s) 634, 2576
PspGI CCWGG 5 cut(s) 295, 988, 1407, 2060, 2107
PspN4I GGNNCC 5 cut(s) 415, 994, 1332, 1617, 2838
PspPI GGNCC 4 cut(s) 992, 1783, 1883, 2160
PspPPI RGGWCCY 1 cut(s) 992
PsrI GAACNNNNNNTAC 2 cut(s) 406, 438
PstI CTGCAG 1 cut(s) 102
PstNI CAGNNNCTG 1 cut(s) 2822
PsuI RGATCY 2 cut(s) 305, 481
PsyI GACNNNGTC 1 cut(s) 2366
PvuI CGATCG 1 cut(s) 738
PvuII CAGCTG 3 cut(s) 638, 1778, 2307
RsaI GTAC 5 cut(s) 1115, 1248, 1324, 2194, 2695
RsaNI GTAC 5 cut(s) 1114, 1247, 1323, 2193, 2694
RseI CAYNNNNRTG 4 cut(s) 974, 1976, 2610, 2862
Rsr2I CGGWCCG 1 cut(s) 1783
RsrII CGGWCCG 1 cut(s) 1783
SaqAI TTAA 6 cut(s) 255, 462, 666, 1029, 1544, 1824
SatI GCNGC 7 cut(s) 812, 904, 1295, 1779, 2742, 2745, 2855
Sau96I GGNCC 4 cut(s) 992, 1783, 1883, 2160
SchI GAGTC 4 cut(s) 164, 549, 1262, 1400
ScrFI CCNGG 8 cut(s) 297, 990, 1396, 1409, 1576, 2062, 2109, 2511
SduI GDGCHC 2 cut(s) 1983, 2572
SexAI ACCWGGT 1 cut(s) 988
SfaNI GCATC 2 cut(s) 1281, 1458
SfcI CTRYAG 2 cut(s) 98, 972
SinI GGWCC 3 cut(s) 992, 1783, 1883
SmiMI CAYNNNNRTG 4 cut(s) 974, 1976, 2610, 2862
SmlI CTYRAG 4 cut(s) 59, 665, 1313, 2794
SmoI CTYRAG 4 cut(s) 59, 665, 1313, 2794
SpeI ACTAGT 1 cut(s) 1381
SphI GCATGC 1 cut(s) 818
SseBI AGGCCT 1 cut(s) 2402
SspMI CTAG 4 cut(s) 309, 485, 1382, 2282
StuI AGGCCT 1 cut(s) 2402
StyD4I CCNGG 8 cut(s) 295, 988, 1394, 1407, 1574, 2060, 2107, 2509
StyI CCWWGG 5 cut(s) 869, 1326, 1538, 1886, 2098
TaaI ACNGT 9 cut(s) 226, 532, 849, 976, 1210, 1929, 2358, 2533, 2818
TaiI ACGT 4 cut(s) 1160, 1252, 1435, 1637
TaqI TCGA 6 cut(s) 266, 558, 1095, 2150, 2361, 2772
TatI WGTACW 1 cut(s) 1113
TauI GCSGC 2 cut(s) 1297, 2747
TfiI GAWTC 6 cut(s) 761, 874, 1097, 1139, 2351, 2461
Tru1I TTAA 6 cut(s) 255, 462, 666, 1029, 1544, 1824
Tru9I TTAA 6 cut(s) 255, 462, 666, 1029, 1544, 1824
TscAI CASTG 4 cut(s) 981, 1129, 1934, 2142
TseFI GTSAC 3 cut(s) 1133, 1190, 1664
TseI GCWGC 5 cut(s) 811, 903, 1778, 2741, 2854
Tsp45I GTSAC 3 cut(s) 1133, 1190, 1664
TspGWI ACGGA 4 cut(s) 848, 1099, 1209, 1652
TspRI CASTG 4 cut(s) 981, 1129, 1934, 2142
Tth111I GACNNNGTC 1 cut(s) 2366
Van91I CCANNNNNTGG 1 cut(s) 302
Vha464I CTTAAG 1 cut(s) 665
VpaK11BI GGWCC 3 cut(s) 992, 1783, 1883
XapI RAATTY 6 cut(s) 68, 360, 497, 1502, 1591, 2474
XbaI TCTAGA 1 cut(s) 308
XceI RCATGY 3 cut(s) 400, 818, 2881
XcmI CCANNNNNNNNNTGG 1 cut(s) 2105
XmiI GTMKAC 1 cut(s) 1376
XmnI GAANNNNTTC 1 cut(s) 2661
XspI CTAG 4 cut(s) 309, 485, 1382, 2282
Zsp2I ATGCAT 1 cut(s) 1274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.