FvH4_1g04640

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001179
Physical Location & Seq
Reverse (-)
2458246 .. 2460791
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04640.t2

Sequence Viewer

Length: 1476 bp
ATGTTAACATGTCAGGAGCTGCTGAAGATGTGTGCTTCGGTATGGCATCCTCTAATCGGCACATTCATGATATCCGTAATGGCTACATACTTGACTGGTGTGTGGGAGCTCAGCATCGTGCATGCTGCTCAAATTCTCAATATTTATTCCGGGGCCGTTGCCATATTGCCGTTGCTTATGCAATTAATTTTCAAAGAAGAGACGCTGGTTAGCCATTCCATGGTTTTGCTCACCAGATTTGCCTATACCACTGGTTTTGCAATGTTGACAATGTCAGCACCACCAATTCTTTCCTTGGCAACCGGCACTTGCAGAGAAGAGGAGGCAGAGTGCATTGGTCAAGTACAAAAAATTCTGTTTTACATAGGATTAACTCTGATAGCTGTTGCAGATTGTGTTCACGAGGTCTCAGCAGGAGAATACTCCTTGACCAATCTGTTGACATCAGCGAGTGACACGGCAGACGAATGGTTTGATGAACAGTACAATGCTTATGGACAGACACATCAGCCGTCATTTTGCCCTACTTTTTGTGTGGCATTAGTGTCTCGGACTAGTTTAACCCTCATCCCGGTAGTAGCAATCCTTGCGATTGGTTATATAGAGGCGTGGTGGATTAAGTTTGGGATCCCAGCCTTGTTTGCCGTAGCATCGTTAGTTGTTATCTTGTTCAGTAGTAAAGTAAATCATCGACCGCCGCAGCAGCCTTCACCTCTACAAGAGTTTTTTTTAGAAGTGATATCTTCTGAGGAAGGAATCTCTCCCCTATGGGTAGTTCCGATATGCATCAACTTTCTCCTCATAAGTGTTGTATTTTCTATGGGAAATACGTTCTTCATTGAACAAGCAACCGACCTGAATCTCTATTTTTGGTTTTTTGAGTTATCAATTACTGCACTTCCATTGATCCAGACGGCAACCAAACTACTTCTGCATATCCCAATGGAGTTGTGTTCTTTAGAGCCCTTGCTTGGGACTTCTGTGTCAATGATATTTGCAACACTGTGTTGCATCACTGCTGCAAAAGTTGAGACCCGAAGGTTGAGCGTGGTTAGCACCGAGGGTGTTATTGATGAGCCTGACGTGATCATTTCTATGTCTATAGGTTGGTTGATTCCACAGTTTATTCTCCTCGGAATGGCTGAAGGCTTTTCGGGGTCGTGCATGGCCCAGTTCTTCAAACAATTACCGGGCCGTGCATCCAAATACTCAGAAGGCATTGCTCAGAGTGTTAATGGAGTCGGAGTTTTGTGCGGTGTGTTGTCAGTTTACATTGTAGGTCAGATACAACCCGCTTGGTTCCAGAGCACATTAAACACGAGTCGTCTGGATAATTACTACTGGACCTTGGCTGCACTATCTGCTGCCAATCTGGTCTTGTTTCCTTTTGGGATATTGTTATACTTAACTGGAATCCCGCAAATTCACCGAGACATATTGAAGGAACGGTTTCAAATGCTGCTATTCTGGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

492

Amino Acids

54.28

Weight (kDa)

4.88

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 982
AccB7I CCANNNNNTGG 1 cut(s) 220
AciI CCGC 5 cut(s) 695, 698, 1254, 1293, 1418
AclWI GGATC 3 cut(s) 622, 635, 901
AcsI RAATTY 3 cut(s) 132, 351, 1422
AcuI CTGAAG 2 cut(s) 44, 1164
AfaI GTAC 2 cut(s) 345, 485
AfiI CCNNNNNNNGG 6 cut(s) 56, 220, 571, 971, 972, 1138
AflIII ACRYGT 1 cut(s) 8
AgsI TTSAA 5 cut(s) 193, 842, 1180, 1441, 1454
AhlI ACTAGT 1 cut(s) 554
AjiI CACGTC 2 cut(s) 1084, 1473
AluBI AGCT 3 cut(s) 19, 109, 383
AluI AGCT 3 cut(s) 19, 109, 383
Alw21I GWGCWC 2 cut(s) 111, 1310
Alw26I GTCTC 5 cut(s) 194, 412, 552, 1025, 1425
AlwI GGATC 3 cut(s) 622, 635, 901
AlwNI CAGNNNCTG 1 cut(s) 19
AoxI GGCC 3 cut(s) 153, 1167, 1192
ApeKI GCWGC 8 cut(s) 19, 125, 700, 703, 1019, 1352, 1364, 1459
ApoI RAATTY 3 cut(s) 132, 351, 1422
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AseI ATTAAT 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 1449
AspS9I GGNCC 4 cut(s) 153, 1168, 1192, 1344
AsuC2I CCSGG 3 cut(s) 151, 572, 1191
AsuHPI GGTGA 3 cut(s) 223, 702, 1418
AvaII GGWCC 1 cut(s) 1344
BamHI GGATCC 1 cut(s) 627
BanII GRGCYC 2 cut(s) 111, 966
BauI CACGAG 2 cut(s) 401, 1318
Bbv12I GWGCWC 2 cut(s) 111, 1310
BbvI GCAGC 8 cut(s) 6, 112, 712, 715, 1006, 1339, 1351, 1446
BceAI ACGGC 7 cut(s) 140, 154, 474, 496, 629, 930, 1179
BclI TGATCA 1 cut(s) 1086
BcnI CCSGG 3 cut(s) 151, 572, 1191
BcoDI GTCTC 5 cut(s) 194, 412, 552, 1025, 1425
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 1 cut(s) 555
BfmI CTRYAG 1 cut(s) 1101
BisI GCNGC 9 cut(s) 20, 126, 698, 701, 704, 1020, 1353, 1365, 1460
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 9 cut(s) 21, 127, 699, 702, 705, 1021, 1354, 1366, 1461
Bme1390I CCNGG 3 cut(s) 151, 572, 1191
Bme18I GGWCC 1 cut(s) 1344
BmgBI CACGTC 2 cut(s) 1084, 1473
BmgT120I GGNCC 4 cut(s) 153, 1168, 1192, 1344
BmiI GGNNCC 3 cut(s) 154, 629, 1301
BmrFI CCNGG 3 cut(s) 151, 572, 1191
BmrI ACTGGG 1 cut(s) 1165
BmsI GCATC 6 cut(s) 55, 123, 659, 795, 1020, 1208
BmuI ACTGGG 1 cut(s) 1165
Bpu1102I GCTNAGC 1 cut(s) 110
BpuMI CCSGG 3 cut(s) 151, 572, 1191
BsaBI GATNNNNATC 1 cut(s) 785
BsaI GGTCTC 2 cut(s) 412, 1025
BsaJI CCNNGG 6 cut(s) 150, 219, 294, 1059, 1132, 1347
BsaXI ACNNNNNCTCC 4 cut(s) 314, 344, 1230, 1260
Bsc4I CCNNNNNNNGG 6 cut(s) 56, 220, 571, 971, 972, 1138
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 5 cut(s) 100, 256, 1171, 1346, 1414
Bse3DI GCAATG 2 cut(s) 267, 1218
Bse8I GATNNNNATC 1 cut(s) 785
BseDI CCNNGG 6 cut(s) 150, 219, 294, 1059, 1132, 1347
BseGI GGATG 3 cut(s) 46, 567, 1199
BseJI GATNNNNATC 1 cut(s) 785
BseLI CCNNNNNNNGG 6 cut(s) 56, 220, 571, 971, 972, 1138
BseMI GCAATG 2 cut(s) 267, 1218
BseMII CTCAG 5 cut(s) 124, 423, 738, 1224, 1238
BseNI ACTGG 5 cut(s) 100, 256, 1171, 1346, 1414
BseRI GAGGAG 3 cut(s) 335, 788, 1121
BseXI GCAGC 8 cut(s) 6, 112, 712, 715, 1006, 1339, 1351, 1446
BseYI CCCAGC 1 cut(s) 631
BsgI GTGCAG 2 cut(s) 879, 1338
Bsh1285I CGRYCG 1 cut(s) 695
BshFI GGCC 3 cut(s) 155, 1169, 1194
BsiEI CGRYCG 1 cut(s) 695
BsiHKAI GWGCWC 2 cut(s) 111, 1310
BsiSI CCGG 4 cut(s) 150, 303, 572, 1190
BslFI GGGAC 1 cut(s) 988
BslI CCNNNNNNNGG 6 cut(s) 56, 220, 571, 971, 972, 1138
BsmAI GTCTC 5 cut(s) 194, 412, 552, 1025, 1425
BsmBI CGTCTC 1 cut(s) 194
BsmFI GGGAC 1 cut(s) 988
BsnI GGCC 3 cut(s) 155, 1169, 1194
Bso31I GGTCTC 2 cut(s) 412, 1025
Bsp1286I GDGCHC 3 cut(s) 111, 966, 1310
Bsp143I GATC 3 cut(s) 627, 906, 1086
Bsp1720I GCTNAGC 1 cut(s) 110
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 5 cut(s) 695, 698, 1254, 1293, 1418
BspANI GGCC 3 cut(s) 155, 1169, 1194
BspCNI CTCAG 5 cut(s) 123, 422, 739, 1223, 1237
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 154, 629, 1301
BspPI GGATC 3 cut(s) 622, 635, 901
BspTNI GGTCTC 2 cut(s) 412, 1025
BsrDI GCAATG 2 cut(s) 267, 1218
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 5 cut(s) 100, 256, 1171, 1346, 1414
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 6 cut(s) 150, 219, 294, 1059, 1132, 1347
BssMI GATC 3 cut(s) 627, 906, 1086
BssSI CACGAG 2 cut(s) 401, 1318
BssT1I CCWWGG 3 cut(s) 219, 294, 1347
Bst2BI CACGAG 2 cut(s) 401, 1318
Bst4CI ACNGT 4 cut(s) 483, 1005, 1122, 1449
Bst6I CTCTTC 2 cut(s) 192, 312
BstAPI GCANNNNNTGC 2 cut(s) 587, 1361
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 5 cut(s) 110, 409, 747, 1210, 1224
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 3 cut(s) 46, 567, 1199
BstKTI GATC 3 cut(s) 630, 909, 1089
BstMAI GTCTC 5 cut(s) 194, 412, 552, 1025, 1425
BstMBI GATC 3 cut(s) 627, 906, 1086
BstMCI CGRYCG 1 cut(s) 695
BstMWI GCNNNNNNNGC 5 cut(s) 587, 641, 703, 1053, 1361
BstNSI RCATGY 2 cut(s) 12, 125
BstSCI CCNGG 3 cut(s) 149, 570, 1189
BstSFI CTRYAG 1 cut(s) 1101
BstV1I GCAGC 8 cut(s) 6, 112, 712, 715, 1006, 1339, 1351, 1446
BstX2I RGATCY 1 cut(s) 627
BstYI RGATCY 1 cut(s) 627
BsuRI GGCC 3 cut(s) 155, 1169, 1194
BtgI CCRYGG 1 cut(s) 219
BtrI CACGTC 2 cut(s) 1084, 1473
BtsCI GGATG 3 cut(s) 46, 567, 1199
BtsI GCAGTG 1 cut(s) 1014
BtsIMutI CAGTG 3 cut(s) 249, 1001, 1014
Cac8I GCNNGC 1 cut(s) 123
CaiI CAGNNNCTG 1 cut(s) 19
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 4 cut(s) 153, 1168, 1192, 1344
CseI GACGC 1 cut(s) 211
Csp6I GTAC 2 cut(s) 344, 484
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 5 cut(s) 9, 67, 122, 220, 1165
CviQI GTAC 2 cut(s) 344, 484
DdeI CTNAG 5 cut(s) 110, 409, 747, 1210, 1224
DpnI GATC 3 cut(s) 629, 908, 1088
DpnII GATC 3 cut(s) 627, 906, 1086
DrdI GACNNNNNNGTC 1 cut(s) 982
DseDI GACNNNNNNGTC 1 cut(s) 982
Eam1104I CTCTTC 2 cut(s) 192, 312
EarI CTCTTC 2 cut(s) 192, 312
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 3 cut(s) 219, 294, 1347
Eco24I GRGCYC 2 cut(s) 111, 966
Eco31I GGTCTC 2 cut(s) 412, 1025
Eco32I GATATC 2 cut(s) 72, 741
Eco47I GGWCC 1 cut(s) 1344
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 2 cut(s) 44, 1164
EcoICRI GAGCTC 1 cut(s) 109
EcoRV GATATC 2 cut(s) 72, 741
EcoT14I CCWWGG 3 cut(s) 219, 294, 1347
EcoT22I ATGCAT 1 cut(s) 788
EcoT38I GRGCYC 2 cut(s) 111, 966
ErhI CCWWGG 3 cut(s) 219, 294, 1347
Esp3I CGTCTC 1 cut(s) 194
FaeI CATG 5 cut(s) 12, 70, 125, 223, 1168
FaqI GGGAC 1 cut(s) 988
FatI CATG 5 cut(s) 8, 66, 121, 219, 1164
FauI CCCGC 2 cut(s) 1300, 1425
FbaI TGATCA 1 cut(s) 1086
Fnu4HI GCNGC 9 cut(s) 20, 126, 698, 701, 704, 1020, 1353, 1365, 1460
FokI GGATG 3 cut(s) 33, 554, 1186
FriOI GRGCYC 2 cut(s) 111, 966
Fsp4HI GCNGC 9 cut(s) 20, 126, 698, 701, 704, 1020, 1353, 1365, 1460
FspBI CTAG 1 cut(s) 555
GluI GCNGC 9 cut(s) 20, 126, 698, 701, 704, 1020, 1353, 1365, 1460
GsaI CCCAGC 1 cut(s) 635
HaeIII GGCC 3 cut(s) 155, 1169, 1194
HapII CCGG 4 cut(s) 150, 303, 572, 1190
HgaI GACGC 1 cut(s) 211
Hin1II CATG 5 cut(s) 12, 70, 125, 223, 1168
HincII GTYRAC 3 cut(s) 6, 267, 441
HindII GTYRAC 3 cut(s) 6, 267, 441
HinfI GANTC 6 cut(s) 756, 859, 1114, 1239, 1321, 1413
HpaI GTTAAC 1 cut(s) 6
HpaII CCGG 4 cut(s) 150, 303, 572, 1190
HphI GGTGA 3 cut(s) 223, 702, 1418
Hpy166II GTNNAC 5 cut(s) 6, 267, 400, 441, 1270
Hpy188I TCNGA 9 cut(s) 378, 552, 748, 780, 1136, 1213, 1227, 1244, 1284
Hpy188III TCNNGA 7 cut(s) 14, 67, 401, 910, 1303, 1328, 1468
Hpy8I GTNNAC 5 cut(s) 6, 267, 400, 441, 1270
HpyAV CCTTC 6 cut(s) 717, 746, 1032, 1139, 1208, 1435
HpyCH4III ACNGT 4 cut(s) 483, 1005, 1122, 1449
HpyCH4IV ACGT 3 cut(s) 830, 1083, 1472
HpyF10VI GCNNNNNNNGC 5 cut(s) 587, 641, 703, 1053, 1361
HpyF3I CTNAG 5 cut(s) 110, 409, 747, 1210, 1224
HpySE526I ACGT 3 cut(s) 830, 1083, 1472
Hsp92II CATG 5 cut(s) 12, 70, 125, 223, 1168
Ksp22I TGATCA 1 cut(s) 1086
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 3 cut(s) 627, 906, 1086
LmnI GCTCC 2 cut(s) 16, 106
Lsp1109I GCAGC 8 cut(s) 6, 112, 712, 715, 1006, 1339, 1351, 1446
LweI GCATC 6 cut(s) 55, 123, 659, 795, 1020, 1208
MaeI CTAG 1 cut(s) 555
MaeII ACGT 3 cut(s) 830, 1083, 1472
MaeIII GTNAC 1 cut(s) 452
MalI GATC 3 cut(s) 629, 908, 1088
MboI GATC 3 cut(s) 627, 906, 1086
MboII GAAGA 6 cut(s) 37, 209, 329, 735, 826, 1168
MflI RGATCY 1 cut(s) 627
MhlI GDGCHC 3 cut(s) 111, 966, 1310
MluCI AATT 9 cut(s) 132, 182, 186, 285, 351, 888, 1184, 1333, 1422
MlyI GAGTC 2 cut(s) 1248, 1330
MmeI TCCRAC 1 cut(s) 1222
Mph1103I ATGCAT 1 cut(s) 788
MroXI GAANNNNTTC 1 cut(s) 1449
MseI TTAA 8 cut(s) 5, 185, 371, 560, 618, 1233, 1313, 1406
MslI CAYNNNNRTG 2 cut(s) 65, 1094
MspI CCGG 4 cut(s) 150, 303, 572, 1190
MspR9I CCNGG 3 cut(s) 151, 572, 1191
MwoI GCNNNNNNNGC 5 cut(s) 587, 641, 703, 1053, 1361
NciI CCSGG 3 cut(s) 151, 572, 1191
NcoI CCATGG 1 cut(s) 219
NdeII GATC 3 cut(s) 627, 906, 1086
NlaIII CATG 5 cut(s) 12, 70, 125, 223, 1168
NlaIV GGNNCC 3 cut(s) 154, 629, 1301
NmuCI GTSAC 1 cut(s) 452
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 2 cut(s) 12, 125
PaeI GCATGC 1 cut(s) 125
PagI TCATGA 1 cut(s) 66
PciI ACATGT 1 cut(s) 8
PdmI GAANNNNTTC 1 cut(s) 1449
PfeI GAWTC 4 cut(s) 756, 859, 1114, 1413
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 9 cut(s) 21, 127, 699, 702, 705, 1021, 1354, 1366, 1461
PleI GAGTC 2 cut(s) 1247, 1329
PpsI GAGTC 2 cut(s) 1247, 1329
PscI ACATGT 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 111
PspFI CCCAGC 1 cut(s) 631
PspN4I GGNNCC 3 cut(s) 154, 629, 1301
PspPI GGNCC 4 cut(s) 153, 1168, 1192, 1344
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 1 cut(s) 627
PsyI GACNNNGTC 1 cut(s) 271
RsaI GTAC 2 cut(s) 345, 485
RsaNI GTAC 2 cut(s) 344, 484
RseI CAYNNNNRTG 2 cut(s) 65, 1094
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 8 cut(s) 5, 185, 371, 560, 618, 1233, 1313, 1406
SatI GCNGC 9 cut(s) 20, 126, 698, 701, 704, 1020, 1353, 1365, 1460
Sau3AI GATC 3 cut(s) 627, 906, 1086
Sau96I GGNCC 4 cut(s) 153, 1168, 1192, 1344
SchI GAGTC 2 cut(s) 1248, 1330
ScrFI CCNGG 3 cut(s) 151, 572, 1191
SduI GDGCHC 3 cut(s) 111, 966, 1310
SfaNI GCATC 6 cut(s) 55, 123, 659, 795, 1020, 1208
SfcI CTRYAG 1 cut(s) 1101
SinI GGWCC 1 cut(s) 1344
SmiMI CAYNNNNRTG 2 cut(s) 65, 1094
SpeI ACTAGT 1 cut(s) 554
SphI GCATGC 1 cut(s) 125
Sse9I AATT 9 cut(s) 132, 182, 186, 285, 351, 888, 1184, 1333, 1422
SsiI CCGC 5 cut(s) 695, 698, 1254, 1293, 1418
SspI AATATT 1 cut(s) 142
SspMI CTAG 1 cut(s) 555
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 3 cut(s) 149, 570, 1189
StyI CCWWGG 3 cut(s) 219, 294, 1347
TaaI ACNGT 4 cut(s) 483, 1005, 1122, 1449
TaiI ACGT 3 cut(s) 833, 1086, 1475
TaqI TCGA 1 cut(s) 691
TasI AATT 9 cut(s) 132, 182, 186, 285, 351, 888, 1184, 1333, 1422
TatI WGTACW 2 cut(s) 343, 483
TauI GCSGC 1 cut(s) 700
TfiI GAWTC 4 cut(s) 756, 859, 1114, 1413
Tru1I TTAA 8 cut(s) 5, 185, 371, 560, 618, 1233, 1313, 1406
Tru9I TTAA 8 cut(s) 5, 185, 371, 560, 618, 1233, 1313, 1406
TscAI CASTG 3 cut(s) 256, 1008, 1021
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 8 cut(s) 19, 125, 700, 703, 1019, 1352, 1364, 1459
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 3 cut(s) 55, 492, 826
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 3 cut(s) 256, 1008, 1021
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 1344
VspI ATTAAT 1 cut(s) 185
XapI RAATTY 3 cut(s) 132, 351, 1422
XceI RCATGY 2 cut(s) 12, 125
XmnI GAANNNNTTC 1 cut(s) 1449
XspI CTAG 1 cut(s) 555
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.