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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 259 |
| AccII | CGCG | 3 cut(s) | 11, 128, 233 |
| AcoI | YGGCCR | 1 cut(s) | 123 |
| AjnI | CCWGG | 1 cut(s) | 26 |
| AoxI | GGCC | 2 cut(s) | 24, 123 |
| ApeKI | GCWGC | 1 cut(s) | 62 |
| AsuHPI | GGTGA | 1 cut(s) | 155 |
| BbvI | GCAGC | 1 cut(s) | 74 |
| BccI | CCATC | 1 cut(s) | 102 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 54, 88 |
| BciT130I | CCWGG | 1 cut(s) | 28 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 131 |
| BisI | GCNGC | 6 cut(s) | 63, 123, 126, 129, 132, 190 |
| BlsI | GCNGC | 6 cut(s) | 64, 124, 127, 130, 133, 191 |
| Bme1390I | CCNGG | 1 cut(s) | 28 |
| BmrFI | CCNGG | 1 cut(s) | 28 |
| BmsI | GCATC | 1 cut(s) | 26 |
| BpuEI | CTTGAG | 1 cut(s) | 239 |
| BsaJI | CCNNGG | 1 cut(s) | 126 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 168, 198 |
| BseBI | CCWGG | 1 cut(s) | 28 |
| BseDI | CCNNGG | 1 cut(s) | 126 |
| BseGI | GGATG | 1 cut(s) | 113 |
| BseX3I | CGGCCG | 1 cut(s) | 123 |
| BseXI | GCAGC | 1 cut(s) | 74 |
| Bsh1236I | CGCG | 3 cut(s) | 11, 128, 233 |
| Bsh1285I | CGRYCG | 2 cut(s) | 126, 237 |
| BshFI | GGCC | 2 cut(s) | 26, 125 |
| BsiEI | CGRYCG | 2 cut(s) | 126, 237 |
| BslFI | GGGAC | 1 cut(s) | 228 |
| BsmFI | GGGAC | 1 cut(s) | 228 |
| BsnI | GGCC | 2 cut(s) | 26, 125 |
| BspANI | GGCC | 2 cut(s) | 26, 125 |
| BspFNI | CGCG | 3 cut(s) | 11, 128, 233 |
| BsrBI | CCGCTC | 1 cut(s) | 259 |
| BssECI | CCNNGG | 1 cut(s) | 126 |
| Bst2UI | CCWGG | 1 cut(s) | 28 |
| BstC8I | GCNNGC | 1 cut(s) | 67 |
| BstDSI | CCRYGG | 1 cut(s) | 126 |
| BstF5I | GGATG | 1 cut(s) | 113 |
| BstFNI | CGCG | 3 cut(s) | 11, 128, 233 |
| BstMCI | CGRYCG | 2 cut(s) | 126, 237 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 23, 128, 131 |
| BstNI | CCWGG | 1 cut(s) | 28 |
| BstNSI | RCATGY | 1 cut(s) | 69 |
| BstSCI | CCNGG | 1 cut(s) | 26 |
| BstUI | CGCG | 3 cut(s) | 11, 128, 233 |
| BstV1I | GCAGC | 1 cut(s) | 74 |
| BstZI | CGGCCG | 1 cut(s) | 123 |
| BsuRI | GGCC | 2 cut(s) | 26, 125 |
| BtgI | CCRYGG | 1 cut(s) | 126 |
| BtsCI | GGATG | 1 cut(s) | 113 |
| Cac8I | GCNNGC | 1 cut(s) | 67 |
| CciNI | GCGGCCGC | 1 cut(s) | 123 |
| Cfr42I | CCGCGG | 1 cut(s) | 129 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 29 |
| CviAII | CATG | 1 cut(s) | 66 |
| CviJI | RGCY | 2 cut(s) | 26, 125 |
| CviKI_1 | RGCY | 2 cut(s) | 26, 125 |
| EaeI | YGGCCR | 1 cut(s) | 123 |
| EagI | CGGCCG | 1 cut(s) | 123 |
| EclXI | CGGCCG | 1 cut(s) | 123 |
| Eco52I | CGGCCG | 1 cut(s) | 123 |
| EcoRII | CCWGG | 1 cut(s) | 26 |
| FaeI | CATG | 1 cut(s) | 69 |
| FaiI | YATR | 1 cut(s) | 67 |
| FaqI | GGGAC | 1 cut(s) | 228 |
| FatI | CATG | 1 cut(s) | 65 |
| FauI | CCCGC | 1 cut(s) | 239 |
| Fnu4HI | GCNGC | 6 cut(s) | 63, 123, 126, 129, 132, 190 |
| FokI | GGATG | 1 cut(s) | 120 |
| Fsp4HI | GCNGC | 6 cut(s) | 63, 123, 126, 129, 132, 190 |
| GluI | GCNGC | 6 cut(s) | 63, 123, 126, 129, 132, 190 |
| HaeIII | GGCC | 2 cut(s) | 26, 125 |
| Hin1II | CATG | 1 cut(s) | 69 |
| HphI | GGTGA | 1 cut(s) | 155 |
| HpyAV | CCTTC | 3 cut(s) | 16, 236, 247 |
| HpyCH4V | TGCA | 2 cut(s) | 62, 69 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 23, 128, 131 |
| Hsp92II | CATG | 1 cut(s) | 69 |
| KspI | CCGCGG | 1 cut(s) | 129 |
| LmnI | GCTCC | 1 cut(s) | 256 |
| LpnPI | CCDG | 4 cut(s) | 13, 40, 152, 189 |
| Lsp1109I | GCAGC | 1 cut(s) | 74 |
| LweI | GCATC | 1 cut(s) | 26 |
| MbiI | CCGCTC | 1 cut(s) | 259 |
| MboII | GAAGA | 1 cut(s) | 64 |
| MnlI | CCTC | 7 cut(s) | 98, 133, 187, 205, 214, 218, 257 |
| MspA1I | CMGCKG | 1 cut(s) | 128 |
| MspR9I | CCNGG | 1 cut(s) | 28 |
| MvaI | CCWGG | 1 cut(s) | 28 |
| MvnI | CGCG | 3 cut(s) | 11, 128, 233 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 23, 128, 131 |
| NlaIII | CATG | 1 cut(s) | 69 |
| NotI | GCGGCCGC | 1 cut(s) | 123 |
| NspI | RCATGY | 1 cut(s) | 69 |
| PaeI | GCATGC | 1 cut(s) | 69 |
| PkrI | GCNGC | 6 cut(s) | 64, 124, 127, 130, 133, 191 |
| Psp6I | CCWGG | 1 cut(s) | 26 |
| PspGI | CCWGG | 1 cut(s) | 26 |
| SacII | CCGCGG | 1 cut(s) | 129 |
| SatI | GCNGC | 6 cut(s) | 63, 123, 126, 129, 132, 190 |
| ScrFI | CCNGG | 1 cut(s) | 28 |
| SetI | ASST | 2 cut(s) | 32, 198 |
| SfaNI | GCATC | 1 cut(s) | 26 |
| Sfr303I | CCGCGG | 1 cut(s) | 129 |
| SgrBI | CCGCGG | 1 cut(s) | 129 |
| SmlI | CTYRAG | 1 cut(s) | 218 |
| SmoI | CTYRAG | 1 cut(s) | 218 |
| SphI | GCATGC | 1 cut(s) | 69 |
| SspI | AATATT | 1 cut(s) | 58 |
| StyD4I | CCNGG | 1 cut(s) | 26 |
| TaqI | TCGA | 4 cut(s) | 20, 74, 198, 237 |
| TauI | GCSGC | 5 cut(s) | 125, 128, 131, 134, 192 |
| TseI | GCWGC | 1 cut(s) | 62 |
| TspDTI | ATGAA | 1 cut(s) | 255 |
| XceI | RCATGY | 1 cut(s) | 69 |