POT family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 227 |
| AcuI | CTGAAG | 1 cut(s) | 90 |
| AfaI | GTAC | 2 cut(s) | 5, 265 |
| AgsI | TTSAA | 4 cut(s) | 34, 140, 150, 176 |
| BclI | TGATCA | 2 cut(s) | 25, 99 |
| BfmI | CTRYAG | 1 cut(s) | 294 |
| BmcAI | AGTACT | 1 cut(s) | 265 |
| BmsI | GCATC | 3 cut(s) | 52, 284, 310 |
| Bse1I | ACTGG | 1 cut(s) | 236 |
| BseNI | ACTGG | 1 cut(s) | 236 |
| Bsp143I | GATC | 4 cut(s) | 25, 48, 99, 300 |
| BspACI | CCGC | 1 cut(s) | 227 |
| BsrI | ACTGG | 1 cut(s) | 236 |
| BssMI | GATC | 4 cut(s) | 25, 48, 99, 300 |
| BstKTI | GATC | 4 cut(s) | 28, 51, 102, 303 |
| BstMBI | GATC | 4 cut(s) | 25, 48, 99, 300 |
| BstSFI | CTRYAG | 1 cut(s) | 294 |
| BtsIMutI | CAGTG | 1 cut(s) | 66 |
| Csp6I | GTAC | 2 cut(s) | 4, 264 |
| CviAII | CATG | 1 cut(s) | 15 |
| CviJI | RGCY | 3 cut(s) | 133, 192, 211 |
| CviKI_1 | RGCY | 3 cut(s) | 133, 192, 211 |
| CviQI | GTAC | 2 cut(s) | 4, 264 |
| DpnI | GATC | 4 cut(s) | 27, 50, 101, 302 |
| DpnII | GATC | 4 cut(s) | 25, 48, 99, 300 |
| Eco57I | CTGAAG | 1 cut(s) | 90 |
| FaeI | CATG | 1 cut(s) | 18 |
| FaiI | YATR | 5 cut(s) | 16, 89, 104, 122, 220 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 200, 232, 253, 285 |
| FatI | CATG | 1 cut(s) | 14 |
| FbaI | TGATCA | 2 cut(s) | 25, 99 |
| Hin1II | CATG | 1 cut(s) | 18 |
| HinfI | GANTC | 2 cut(s) | 34, 290 |
| Hpy188I | TCNGA | 1 cut(s) | 25 |
| Hpy188III | TCNNGA | 2 cut(s) | 187, 279 |
| HpyAV | CCTTC | 3 cut(s) | 37, 156, 223 |
| HpyCH4V | TGCA | 4 cut(s) | 120, 275, 317, 323 |
| Hsp92II | CATG | 1 cut(s) | 18 |
| Ksp22I | TGATCA | 2 cut(s) | 25, 99 |
| Kzo9I | GATC | 4 cut(s) | 25, 48, 99, 300 |
| LpnPI | CCDG | 1 cut(s) | 249 |
| LweI | GCATC | 3 cut(s) | 52, 284, 310 |
| MalI | GATC | 4 cut(s) | 27, 50, 101, 302 |
| MboI | GATC | 4 cut(s) | 25, 48, 99, 300 |
| MboII | GAAGA | 2 cut(s) | 83, 106 |
| MfeI | CAATTG | 1 cut(s) | 29 |
| MluCI | AATT | 4 cut(s) | 29, 256, 307, 325 |
| MnlI | CCTC | 3 cut(s) | 203, 213, 244 |
| MseI | TTAA | 2 cut(s) | 260, 328 |
| MslI | CAYNNNNRTG | 2 cut(s) | 115, 125 |
| MunI | CAATTG | 1 cut(s) | 29 |
| NdeII | GATC | 4 cut(s) | 25, 48, 99, 300 |
| NlaIII | CATG | 1 cut(s) | 18 |
| PfeI | GAWTC | 2 cut(s) | 34, 290 |
| RsaI | GTAC | 2 cut(s) | 5, 265 |
| RsaNI | GTAC | 2 cut(s) | 4, 264 |
| RseI | CAYNNNNRTG | 2 cut(s) | 115, 125 |
| SaqAI | TTAA | 2 cut(s) | 260, 328 |
| Sau3AI | GATC | 4 cut(s) | 25, 48, 99, 300 |
| ScaI | AGTACT | 1 cut(s) | 265 |
| SetI | ASST | 1 cut(s) | 205 |
| SfaNI | GCATC | 3 cut(s) | 52, 284, 310 |
| SfcI | CTRYAG | 1 cut(s) | 294 |
| SgeI | CNNG | 7 cut(s) | 27, 50, 185, 199, 248, 282, 291 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 115, 125 |
| Sse9I | AATT | 4 cut(s) | 29, 256, 307, 325 |
| SsiI | CCGC | 1 cut(s) | 227 |
| TaqI | TCGA | 2 cut(s) | 51, 186 |
| TasI | AATT | 4 cut(s) | 29, 256, 307, 325 |
| TatI | WGTACW | 2 cut(s) | 3, 263 |
| TfiI | GAWTC | 2 cut(s) | 34, 290 |
| Tru1I | TTAA | 2 cut(s) | 260, 328 |
| Tru9I | TTAA | 2 cut(s) | 260, 328 |
| TscAI | CASTG | 1 cut(s) | 73 |
| TspDTI | ATGAA | 3 cut(s) | 90, 95, 143 |
| TspRI | CASTG | 1 cut(s) | 73 |
| ZrmI | AGTACT | 1 cut(s) | 265 |