FvH4_3g33690

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001179
Physical Location & Seq
Reverse (-)
29208038 .. 29210507
2470 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33690.t1

Sequence Viewer

Length: 1812 bp
ATGACGAATTTACTGACTGGATATGCAATGAGCATAATGATTACATACTTGAATAGTGTATGGAAGTTCAATCTCACAGATGCTGCTGCCGTCGTCAATGTGTATGCCGGCGCAGTGGCTGTAATGCCTATGGGAATGCTTGTGCTTGTTGATAATGTAATCGGTAGCTATAGGTTGATCATACTCTGCGGTTGTGCTTACACCATGGGTTTTCTCCTCTTGACCATGTCAACACCACCAGTTCTTTATAGGGCAACAGGCACTTGCAGTGCATATGAGGCCGAGTGCATTGGCAAAGTGCAAAAGATTCTCTTTTACACAGCTTTGCCTCTAATAGCTCTGGGAGCCTCTAGTCGCTTTATGGTTGATGCATTCAGGAAAGAGCAGTTGTTCAAGCCACTATTACAAGGCGCCAAAAAGATGAAGTTCTTTGCTCTCTCAGTGTGCCCTAAGAAATTTAGGTTGATTTCATTTCAAATTCTGATCTCTGCCCTAGTACTTGGGGCAACTGCTTATATAAAACCTTGGTCACTTAGGTTTGGTTTGCCTGCTACATTTACCTTGCTTGCAACAATTACACTATTGACAAGTACTATACGTCCACTCAAAGCACAAAGGAACCTTTTCATCAGGGAAGAAATCATAAGCATATTGCGGGTGAGTCCTATATGTATCTTCCTGATTCCAATCGGTGTCGTATCGTCACTCGGAAATACATATTTCACTGAACAAGCAAGCAAGATGAATCACAGGGTTGGATCGTTAAAGGTTTCTGTTCTTTTCCTTACAATGCCCCGAAGCTTGGTCAGATCATTGTTTAGCCAATCAAATTGTTGTGCTAAGATTATTACTCCCAGAATTGGGATTTCGTTATCGATGGCATTGGCGATTGTATGTTGTATCACAGCTGCGTTAGTGGAAAACAGAAGGTTGGGAGTGGTTAAGAGACATGGTTTACTTGACAAGCCTGATGAACCAGTTCCCATGACCATGTTTTGGTTGCTTCCACAGTTTCTTCTGGTTGGAGGCGTTTGTGGGCTTTCTGAGAAGAGCATTGCTCTTTTCTACACTGATGTGGTCCTTCGGTGGCCGAAGTTATTAAAAGATGTTGATATGCAAGCAAAGGAAAAATCCAAGTCCCCCATGGATGAAGCAAAAGTATTCAAGCCTAAAAAGAATGATGAGGACGACGGCAGTCAGGAAAAACAAGAATCAAAGAGTACAAAAAAGGATGATGATGAGGAGAACATTGATCCCGAAAGGGAAAAACAAAAACTGAAGCGTGTATACATAGATTTCTTTGTTGATGCTCTTGAAGGAGTGGGAAATATAGGCAGTGTGGTGATAGTCTATGTAGTGGAAGAGATAAAACCTACTTGGTTTCAAGAAACGGTAAACAATAGTCGTTTGGATAATTACTACTGGACCTTGGCTGCATTGACTGCAACAAATTTGGTAATTTTTATTCCAGTAATATTCTACCTGTTGAGGGTTAATGCTGAGGCTGGGGCAGAAGTTGTTGACTTAGACGATGAGGAAGAAGAGGTTGATGCAGCCGGTGAGGATTTTGTCGTTGAAGCAGCGGCTGAGAGAGTAGAGCAGGGTGAGGCAGCCGGTGAGGAAGGAAAGGCCGGTGCAGCCGATGAGGAAGGGAAGGCTGGTGCAGCCGATGAGGAAGGAAAGGTTACACCAATCTATGTGGAAGCAAAACTCGATATGCCTGAGCAGAAAGAAGAAAAGAAGAAGGGAAAAAATCAGTTGGGTGATATGGATGAGAAGCAGAAGAAAGAACAAAGGGATATGAATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

604

Amino Acids

66.94

Weight (kDa)

5.83

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 410
AccB7I CCANNNNNTGG 1 cut(s) 996
AccI GTMKAC 1 cut(s) 1287
AciI CCGC 3 cut(s) 189, 655, 1583
AclWI GGATC 2 cut(s) 766, 1247
AcoI YGGCCR 1 cut(s) 1088
AcsI RAATTY 4 cut(s) 7, 455, 477, 1450
AcuI CTGAAG 1 cut(s) 1298
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 3 cut(s) 498, 592, 1222
AfiI CCNNNNNNNGG 5 cut(s) 802, 860, 861, 996, 1489
AgsI TTSAA 8 cut(s) 52, 70, 394, 476, 1165, 1316, 1385, 1577
AleI CACNNNNGTG 1 cut(s) 1073
AluBI AGCT 5 cut(s) 168, 323, 338, 801, 908
AluI AGCT 5 cut(s) 168, 323, 338, 801, 908
Alw26I GTCTC 1 cut(s) 940
AlwI GGATC 2 cut(s) 766, 1247
AlwNI CAGNNNCTG 3 cut(s) 83, 119, 1586
AoxI GGCC 3 cut(s) 279, 1088, 1629
ApeKI GCWGC 9 cut(s) 83, 86, 908, 1433, 1553, 1580, 1610, 1637, 1664
ApoI RAATTY 4 cut(s) 7, 455, 477, 1450
Asp700I GAANNNNTTC 2 cut(s) 623, 978
AspLEI GCGC 2 cut(s) 113, 413
AspS9I GGNCC 2 cut(s) 1078, 1425
AsuHPI GGTGA 6 cut(s) 670, 1354, 1571, 1616, 1628, 1775
AvaII GGWCC 2 cut(s) 1078, 1425
BaeGI GKGCMC 1 cut(s) 449
BanI GGYRCC 1 cut(s) 410
BarI GAAGNNNNNNTAC 2 cut(s) 1271, 1303
BbvCI CCTCAGC 1 cut(s) 1500
BbvI GCAGC 9 cut(s) 70, 73, 895, 1420, 1565, 1592, 1622, 1649, 1676
BccI CCATC 1 cut(s) 871
BceAI ACGGC 2 cut(s) 74, 1207
BclI TGATCA 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 940
BfaI CTAG 2 cut(s) 351, 494
BfmI CTRYAG 1 cut(s) 169
BfoI RGCGCY 1 cut(s) 414
BmcAI AGTACT 2 cut(s) 498, 592
Bme18I GGWCC 2 cut(s) 1078, 1425
BmgT120I GGNCC 2 cut(s) 1078, 1425
BmiI GGNNCC 3 cut(s) 346, 412, 620
BmsI GCATC 4 cut(s) 70, 358, 1297, 1540
BoxI GACNNNNGTC 1 cut(s) 1194
BplI GAGNNNNNCTC 2 cut(s) 1042, 1074
Bpu10I CCTNAGC 2 cut(s) 1500, 1722
Bsa29I ATCGAT 1 cut(s) 875
BsaBI GATNNNNATC 2 cut(s) 686, 1803
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 4 cut(s) 204, 524, 1143, 1428
Bsc4I CCNNNNNNNGG 5 cut(s) 802, 860, 861, 996, 1489
Bse118I RCCGGY 4 cut(s) 107, 1556, 1613, 1631
Bse1I ACTGG 5 cut(s) 22, 239, 977, 1427, 1469
Bse3DI GCAATG 2 cut(s) 33, 1053
Bse8I GATNNNNATC 2 cut(s) 686, 1803
BseCI ATCGAT 1 cut(s) 875
BseDI CCNNGG 4 cut(s) 204, 524, 1143, 1428
BseGI GGATG 3 cut(s) 1153, 1237, 1777
BseJI GATNNNNATC 2 cut(s) 686, 1803
BseLI CCNNNNNNNGG 5 cut(s) 802, 860, 861, 996, 1489
BseMI GCAATG 2 cut(s) 33, 1053
BseMII CTCAG 5 cut(s) 453, 1035, 1491, 1578, 1713
BseNI ACTGG 5 cut(s) 22, 239, 977, 1427, 1469
BseRI GAGGAG 2 cut(s) 206, 1256
BseSI GKGCMC 1 cut(s) 449
BseXI GCAGC 9 cut(s) 70, 73, 895, 1420, 1565, 1592, 1622, 1649, 1676
BseYI CCCAGC 1 cut(s) 1505
BsgI GTGCAG 2 cut(s) 1656, 1683
BshFI GGCC 3 cut(s) 281, 1090, 1631
BshNI GGYRCC 1 cut(s) 410
BshVI ATCGAT 1 cut(s) 875
BsiSI CCGG 4 cut(s) 108, 1557, 1614, 1632
BslFI GGGAC 1 cut(s) 1123
BslI CCNNNNNNNGG 5 cut(s) 802, 860, 861, 996, 1489
BsmAI GTCTC 1 cut(s) 940
BsmFI GGGAC 1 cut(s) 1123
BsmI GAATGC 2 cut(s) 141, 371
BsnI GGCC 3 cut(s) 281, 1090, 1631
Bsp1286I GDGCHC 1 cut(s) 449
Bsp143I GATC 5 cut(s) 177, 483, 758, 809, 1252
Bsp19I CCATGG 2 cut(s) 204, 1143
BspACI CCGC 3 cut(s) 189, 655, 1583
BspANI GGCC 3 cut(s) 281, 1090, 1631
BspCNI CTCAG 5 cut(s) 452, 1036, 1492, 1579, 1714
BspDI ATCGAT 1 cut(s) 875
BspLI GGNNCC 3 cut(s) 346, 412, 620
BspPI GGATC 2 cut(s) 766, 1247
BspQI GCTCTTC 1 cut(s) 1043
BspT107I GGYRCC 1 cut(s) 410
BsrDI GCAATG 2 cut(s) 33, 1053
BsrFI RCCGGY 4 cut(s) 107, 1556, 1613, 1631
BsrI ACTGG 5 cut(s) 22, 239, 977, 1427, 1469
BssAI RCCGGY 4 cut(s) 107, 1556, 1613, 1631
BssECI CCNNGG 4 cut(s) 204, 524, 1143, 1428
BssMI GATC 5 cut(s) 177, 483, 758, 809, 1252
BssNAI GTATAC 1 cut(s) 1288
BssNI GRCGYC 1 cut(s) 411
BssT1I CCWWGG 4 cut(s) 204, 524, 1143, 1428
Bst1107I GTATAC 1 cut(s) 1288
Bst4CI ACNGT 2 cut(s) 1011, 1393
Bst6I CTCTTC 3 cut(s) 1043, 1356, 1536
BstACI GRCGYC 1 cut(s) 411
BstAPI GCANNNNNTGC 1 cut(s) 1442
BstC8I GCNNGC 5 cut(s) 109, 549, 567, 736, 1119
BstDEI CTNAG 9 cut(s) 439, 450, 533, 840, 1044, 1500, 1525, 1587, 1722
BstDSI CCRYGG 2 cut(s) 204, 1143
BstF5I GGATG 3 cut(s) 1153, 1237, 1777
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 2 cut(s) 113, 413
BstKTI GATC 5 cut(s) 180, 486, 761, 812, 1255
BstMAI GTCTC 1 cut(s) 940
BstMBI GATC 5 cut(s) 177, 483, 758, 809, 1252
BstMWI GCNNNNNNNGC 5 cut(s) 278, 344, 1442, 1637, 1664
BstPAI GACNNNNGTC 1 cut(s) 1194
BstSFI CTRYAG 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 449
BstV1I GCAGC 9 cut(s) 70, 73, 895, 1420, 1565, 1592, 1622, 1649, 1676
BstZ17I GTATAC 1 cut(s) 1288
Bsu15I ATCGAT 1 cut(s) 875
BsuRI GGCC 3 cut(s) 281, 1090, 1631
BsuTUI ATCGAT 1 cut(s) 875
BtgI CCRYGG 2 cut(s) 204, 1143
BtsCI GGATG 3 cut(s) 1153, 1237, 1777
BtsI GCAGTG 3 cut(s) 120, 274, 1342
BtsIMutI CAGTG 6 cut(s) 120, 274, 447, 723, 1068, 1342
Cac8I GCNNGC 5 cut(s) 109, 549, 567, 736, 1119
CaiI CAGNNNCTG 3 cut(s) 83, 119, 1586
CfoI GCGC 2 cut(s) 113, 413
Cfr10I RCCGGY 4 cut(s) 107, 1556, 1613, 1631
Cfr13I GGNCC 2 cut(s) 1078, 1425
ClaI ATCGAT 1 cut(s) 875
Csp6I GTAC 3 cut(s) 497, 591, 1221
CspCI CAANNNNNGTGG 2 cut(s) 1680, 1715
CviAII CATG 6 cut(s) 205, 226, 950, 985, 991, 1144
CviQI GTAC 3 cut(s) 497, 591, 1221
DdeI CTNAG 9 cut(s) 439, 450, 533, 840, 1044, 1500, 1525, 1587, 1722
DinI GGCGCC 1 cut(s) 412
DpnI GATC 5 cut(s) 179, 485, 760, 811, 1254
DpnII GATC 5 cut(s) 177, 483, 758, 809, 1252
EaeI YGGCCR 1 cut(s) 1088
Eam1104I CTCTTC 3 cut(s) 1043, 1356, 1536
EarI CTCTTC 3 cut(s) 1043, 1356, 1536
Eco130I CCWWGG 4 cut(s) 204, 524, 1143, 1428
Eco47I GGWCC 2 cut(s) 1078, 1425
Eco57I CTGAAG 1 cut(s) 1298
EcoT14I CCWWGG 4 cut(s) 204, 524, 1143, 1428
EcoT22I ATGCAT 1 cut(s) 373
EgeI GGCGCC 1 cut(s) 412
EheI GGCGCC 1 cut(s) 412
ErhI CCWWGG 4 cut(s) 204, 524, 1143, 1428
FaeI CATG 6 cut(s) 208, 229, 953, 988, 994, 1147
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 1 cut(s) 1123
FatI CATG 6 cut(s) 204, 225, 949, 984, 990, 1143
FauI CCCGC 1 cut(s) 648
FauNDI CATATG 1 cut(s) 274
FbaI TGATCA 1 cut(s) 177
FblI GTMKAC 1 cut(s) 1287
FokI GGATG 3 cut(s) 1160, 1244, 1784
FspBI CTAG 2 cut(s) 351, 494
GlaI GCGC 2 cut(s) 112, 412
GsaI CCCAGC 1 cut(s) 1509
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 3 cut(s) 281, 1090, 1631
HapII CCGG 4 cut(s) 108, 1557, 1614, 1632
HhaI GCGC 2 cut(s) 113, 413
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 6 cut(s) 208, 229, 953, 988, 994, 1147
Hin6I GCGC 2 cut(s) 111, 411
HinP1I GCGC 2 cut(s) 111, 411
HincII GTYRAC 2 cut(s) 231, 1522
HindII GTYRAC 2 cut(s) 231, 1522
HindIII AAGCTT 1 cut(s) 799
HinfI GANTC 6 cut(s) 307, 661, 682, 745, 1211, 1804
HpaII CCGG 4 cut(s) 108, 1557, 1614, 1632
HphI GGTGA 6 cut(s) 670, 1354, 1571, 1616, 1628, 1775
Hpy166II GTNNAC 6 cut(s) 231, 602, 956, 1288, 1396, 1522
Hpy188I TCNGA 4 cut(s) 483, 710, 809, 1045
Hpy188III TCNNGA 7 cut(s) 220, 376, 679, 1199, 1256, 1313, 1385
Hpy8I GTNNAC 6 cut(s) 231, 602, 956, 1288, 1396, 1522
Hpy99I CGWCG 2 cut(s) 95, 1193
HpyAV CCTTC 8 cut(s) 921, 1091, 1310, 1616, 1643, 1648, 1670, 1738
HpyCH4III ACNGT 2 cut(s) 1011, 1393
HpyCH4IV ACGT 1 cut(s) 598
HpyF10VI GCNNNNNNNGC 5 cut(s) 278, 344, 1442, 1637, 1664
HpyF3I CTNAG 9 cut(s) 439, 450, 533, 840, 1044, 1500, 1525, 1587, 1722
HpySE526I ACGT 1 cut(s) 598
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 6 cut(s) 208, 229, 953, 988, 994, 1147
HspAI GCGC 2 cut(s) 111, 411
KasI GGCGCC 1 cut(s) 410
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 177
Kzo9I GATC 5 cut(s) 177, 483, 758, 809, 1252
LguI GCTCTTC 1 cut(s) 1043
LmnI GCTCC 1 cut(s) 344
Lsp1109I GCAGC 9 cut(s) 70, 73, 895, 1420, 1565, 1592, 1622, 1649, 1676
LweI GCATC 4 cut(s) 70, 358, 1297, 1540
MaeI CTAG 2 cut(s) 351, 494
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 3 cut(s) 528, 702, 1684
MalI GATC 5 cut(s) 179, 485, 760, 811, 1254
MboI GATC 5 cut(s) 177, 483, 758, 809, 1252
MhlI GDGCHC 1 cut(s) 449
MluCI AATT 9 cut(s) 7, 455, 477, 573, 829, 858, 1414, 1450, 1458
Mly113I GGCGCC 1 cut(s) 411
MlyI GAGTC 1 cut(s) 670
MmeI TCCRAC 2 cut(s) 736, 1003
Mph1103I ATGCAT 1 cut(s) 373
MroNI GCCGGC 1 cut(s) 107
MroXI GAANNNNTTC 2 cut(s) 623, 978
MseI TTAA 5 cut(s) 764, 942, 1100, 1494, 1810
MslI CAYNNNNRTG 2 cut(s) 989, 1073
MspA1I CMGCKG 2 cut(s) 908, 1583
MspI CCGG 4 cut(s) 108, 1557, 1614, 1632
Mva1269I GAATGC 2 cut(s) 141, 371
MwoI GCNNNNNNNGC 5 cut(s) 278, 344, 1442, 1637, 1664
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 1 cut(s) 411
NcoI CCATGG 2 cut(s) 204, 1143
NdeI CATATG 1 cut(s) 274
NdeII GATC 5 cut(s) 177, 483, 758, 809, 1252
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 6 cut(s) 208, 229, 953, 988, 994, 1147
NlaIV GGNNCC 3 cut(s) 346, 412, 620
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 2 cut(s) 528, 702
NsiI ATGCAT 1 cut(s) 373
OliI CACNNNNGTG 1 cut(s) 1073
PciSI GCTCTTC 1 cut(s) 1043
PctI GAATGC 2 cut(s) 141, 371
PdiI GCCGGC 1 cut(s) 109
PdmI GAANNNNTTC 2 cut(s) 623, 978
PfeI GAWTC 5 cut(s) 307, 682, 745, 1211, 1804
PflFI GACNNNGTC 1 cut(s) 226
PflMI CCANNNNNTGG 1 cut(s) 996
PleI GAGTC 1 cut(s) 669
PluTI GGCGCC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 669
PshAI GACNNNNGTC 1 cut(s) 1194
PspFI CCCAGC 1 cut(s) 1505
PspN4I GGNNCC 3 cut(s) 346, 412, 620
PspPI GGNCC 2 cut(s) 1078, 1425
PstNI CAGNNNCTG 3 cut(s) 83, 119, 1586
PsyI GACNNNGTC 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 3 cut(s) 498, 592, 1222
RsaNI GTAC 3 cut(s) 497, 591, 1221
RseI CAYNNNNRTG 2 cut(s) 989, 1073
SapI GCTCTTC 1 cut(s) 1043
SaqAI TTAA 5 cut(s) 764, 942, 1100, 1494, 1810
Sau3AI GATC 5 cut(s) 177, 483, 758, 809, 1252
Sau96I GGNCC 2 cut(s) 1078, 1425
ScaI AGTACT 2 cut(s) 498, 592
SchI GAGTC 1 cut(s) 670
SduI GDGCHC 1 cut(s) 449
SfaNI GCATC 4 cut(s) 70, 358, 1297, 1540
SfcI CTRYAG 1 cut(s) 169
SfoI GGCGCC 1 cut(s) 412
SinI GGWCC 2 cut(s) 1078, 1425
SmiMI CAYNNNNRTG 2 cut(s) 989, 1073
Sse9I AATT 9 cut(s) 7, 455, 477, 573, 829, 858, 1414, 1450, 1458
SsiI CCGC 3 cut(s) 189, 655, 1583
SspDI GGCGCC 1 cut(s) 410
SspI AATATT 1 cut(s) 1476
SspMI CTAG 2 cut(s) 351, 494
StyI CCWWGG 4 cut(s) 204, 524, 1143, 1428
TaaI ACNGT 2 cut(s) 1011, 1393
TaiI ACGT 1 cut(s) 601
TaqI TCGA 2 cut(s) 875, 1713
TasI AATT 9 cut(s) 7, 455, 477, 573, 829, 858, 1414, 1450, 1458
TatI WGTACW 3 cut(s) 496, 590, 1220
TauI GCSGC 1 cut(s) 1586
TfiI GAWTC 5 cut(s) 307, 682, 745, 1211, 1804
Tru1I TTAA 5 cut(s) 764, 942, 1100, 1494, 1810
Tru9I TTAA 5 cut(s) 764, 942, 1100, 1494, 1810
TscAI CASTG 6 cut(s) 120, 274, 447, 730, 1075, 1342
TseFI GTSAC 2 cut(s) 528, 702
TseI GCWGC 9 cut(s) 83, 86, 908, 1433, 1553, 1580, 1610, 1637, 1664
Tsp45I GTSAC 2 cut(s) 528, 702
TspDTI ATGAA 6 cut(s) 437, 459, 616, 758, 987, 1164
TspRI CASTG 6 cut(s) 120, 274, 447, 730, 1075, 1342
Tth111I GACNNNGTC 1 cut(s) 226
Van91I CCANNNNNTGG 1 cut(s) 996
VpaK11BI GGWCC 2 cut(s) 1078, 1425
XapI RAATTY 4 cut(s) 7, 455, 477, 1450
XcmI CCANNNNNNNNNTGG 1 cut(s) 1141
XmiI GTMKAC 1 cut(s) 1287
XmnI GAANNNNTTC 2 cut(s) 623, 978
XspI CTAG 2 cut(s) 351, 494
ZrmI AGTACT 2 cut(s) 498, 592
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.