Rh5AG400000

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001179
Physical Location & Seq
Reverse (-)
69291344 .. 69294817
3474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG400000.1

Sequence Viewer

Length: 1362 bp
ATGATTTTGGTTCAATGGATGCAGGGGTTTTTCCTTTTGACCATGTCAACACCACCAGTTCTTTATAGGGCAACAGGCACTTGTGGCGCATATGAGGCCGAGTGCATTGGCAAAGTACAAAAGATTCTCTTTTACACAGCCTTAACTCTAATAGCTCTGGGAGCCTCTGGTCGCTTTATGGTTCGTGCATTCAGAGAAGAGCAGTTGATCAAGCCACTATTAGAAGGCGCCAAAAAAATGAAGTTCTTTGCTCTGTCAGTGTGCCCTAAGAAAGTAAGGTTGCTGTCATTTCAAATTCTGGTCTCCACCCTGGTACTTGGGACAACTGCTTATTTAAAGCCTTGGTCGCTTAGGTTTGGGTCCCCTGCTATATTTACCTTGCTTGCAACAATTACTTTATTGACAAGTACTATACGTCCACACAAAGCACAAAGAAACCTTTTCACCAGGGAAGAAATCAGAAGCATCTTGCGCATGAGTCCTATATGTATTTTCCTTATTCCAATTGGTGTTGTATCGTCTCTTGGAAATACTTATTTCACTGAACAAGCAAGCAAGATGAATCACAGGGTTGGATCCTTAAAGGTTTCTGTTCTTTTCCTTATAGTGCCCCGAAGTTTGATGAGAAAATGGTTTAGCCAATCAAAATATTGTGCTAATAAGATGATTACTCCAAGAATTGGGATTTCATTGTCGATGGCATTGGCGATTCTATGTTGTATCACAGCTGCATTAGTGGAAAACAGAAGGTTGGGCGTGGTTAAGAGACATGGTTTACTTAACAAGCCCGATGAGACAGTTCCCATGACCATGTTCTGGTTGCTTCCACAGTTTCTTCTCGTTGGAGGAGTTTCTGGGCTTTCCGAGAAGTGCATTGCTCTTTTCTACACTGATGTGGTGCTTCACTGGCCGAAAATAGAAAAAGATGTCGATATACAAGCAAATGACAAACCCAATTCCAAAGAACAAAACATTAAGTCCCCCATGGAACAGGCAAACGAAACACTCAAGCCTACAGAAAAAAATGATGAGGACCCCAATCAGGCAAAACAAGAAACAAAGAGTACGGAAAAAGATGGTGAGGGGCACATTGATCCCGAAAGGGCAAAACAAAAATCCAAGCGTCAATACATAGAGTTTTTTGTTGATGCTCTTGAGGGAATGGGAAATATAGGCAGTGTGGTGTTGGTCTATGTAGTGGGTGTAATAAAACCTACTTGGTTCCAAGAAACGGTAAACCGAAGTCATTTGGATAATTATTACTGGACTTTGGCTGCATTGACTGCAGCTAATTTGATATTGTTTATTCCAGTAATATTCTACCTGTTGAGGTCAAAGAAATTCACTGCATTGTTTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

453

Amino Acids

51.1

Weight (kDa)

9.62

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 150 - 282 1.3e-07 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 227
AccB7I CCANNNNNTGG 2 cut(s) 680, 816
AclWI GGATC 3 cut(s) 570, 583, 1088
AcoI YGGCCR 1 cut(s) 908
AcsI RAATTY 2 cut(s) 294, 1340
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 4 cut(s) 117, 315, 409, 1066
AfiI CCNNNNNNNGG 4 cut(s) 680, 816, 1042, 1231
AgsI TTSAA 2 cut(s) 14, 293
AjnI CCWGG 2 cut(s) 309, 446
AleI CACNNNNGTG 1 cut(s) 893
AluBI AGCT 3 cut(s) 155, 728, 1289
AluI AGCT 3 cut(s) 155, 728, 1289
Alw26I GTCTC 4 cut(s) 307, 525, 760, 788
AlwI GGATC 3 cut(s) 570, 583, 1088
AoxI GGCC 2 cut(s) 96, 908
ApeKI GCWGC 3 cut(s) 728, 1274, 1286
ApoI RAATTY 2 cut(s) 294, 1340
AspLEI GCGC 3 cut(s) 89, 230, 474
AspS9I GGNCC 2 cut(s) 360, 1033
AsuHPI GGTGA 2 cut(s) 436, 1091
AvaII GGWCC 2 cut(s) 360, 1033
BaeGI GKGCMC 3 cut(s) 266, 612, 1089
BamHI GGATCC 1 cut(s) 575
BanI GGYRCC 1 cut(s) 227
BbvI GCAGC 3 cut(s) 715, 1261, 1298
BccI CCATC 2 cut(s) 691, 1070
BciT130I CCWGG 2 cut(s) 311, 448
BclI TGATCA 1 cut(s) 207
BcoDI GTCTC 4 cut(s) 307, 525, 760, 788
BfmI CTRYAG 2 cut(s) 1014, 1284
BfoI RGCGCY 1 cut(s) 231
BisI GCNGC 3 cut(s) 729, 1275, 1287
BlsI GCNGC 3 cut(s) 730, 1276, 1288
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 2 cut(s) 311, 448
Bme18I GGWCC 2 cut(s) 360, 1033
BmgT120I GGNCC 2 cut(s) 360, 1033
BmiI GGNNCC 7 cut(s) 163, 229, 361, 362, 577, 1035, 1223
BmrFI CCNGG 2 cut(s) 311, 448
BmsI GCATC 3 cut(s) 9, 474, 1138
Bpu10I CCTNAGC 1 cut(s) 350
BpuEI CTTGAG 2 cut(s) 992, 1175
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 307
BsaJI CCNNGG 4 cut(s) 309, 341, 447, 984
Bsc4I CCNNNNNNNGG 4 cut(s) 680, 816, 1042, 1231
Bse1I ACTGG 4 cut(s) 56, 911, 1268, 1310
Bse3DI GCAATG 1 cut(s) 873
BseBI CCWGG 2 cut(s) 311, 448
BseDI CCNNGG 4 cut(s) 309, 341, 447, 984
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 4 cut(s) 680, 816, 1042, 1231
BseMI GCAATG 1 cut(s) 873
BseNI ACTGG 4 cut(s) 56, 911, 1268, 1310
BseRI GAGGAG 1 cut(s) 861
BseSI GKGCMC 3 cut(s) 266, 612, 1089
BseXI GCAGC 3 cut(s) 715, 1261, 1298
BshFI GGCC 2 cut(s) 98, 910
BshNI GGYRCC 1 cut(s) 227
BslFI GGGAC 3 cut(s) 334, 346, 964
BslI CCNNNNNNNGG 4 cut(s) 680, 816, 1042, 1231
BsmAI GTCTC 4 cut(s) 307, 525, 760, 788
BsmBI CGTCTC 1 cut(s) 525
BsmFI GGGAC 3 cut(s) 334, 346, 964
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 2 cut(s) 98, 910
Bso31I GGTCTC 1 cut(s) 307
Bsp1286I GDGCHC 3 cut(s) 266, 612, 1089
Bsp143I GATC 3 cut(s) 207, 575, 1093
Bsp19I CCATGG 1 cut(s) 984
BspANI GGCC 2 cut(s) 98, 910
BspLI GGNNCC 7 cut(s) 163, 229, 361, 362, 577, 1035, 1223
BspMAI CTGCAG 1 cut(s) 1288
BspPI GGATC 3 cut(s) 570, 583, 1088
BspQI GCTCTTC 1 cut(s) 192
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 307
BsrDI GCAATG 1 cut(s) 873
BsrI ACTGG 4 cut(s) 56, 911, 1268, 1310
BssECI CCNNGG 4 cut(s) 309, 341, 447, 984
BssMI GATC 3 cut(s) 207, 575, 1093
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 341, 984
Bst2UI CCWGG 2 cut(s) 311, 448
Bst4CI ACNGT 3 cut(s) 799, 831, 1234
Bst6I CTCTTC 1 cut(s) 192
BstACI GRCGYC 1 cut(s) 228
BstAPI GCANNNNNTGC 2 cut(s) 1283, 1355
BstC8I GCNNGC 2 cut(s) 384, 553
BstDEI CTNAG 2 cut(s) 267, 350
BstDSI CCRYGG 1 cut(s) 984
BstF5I GGATG 1 cut(s) 24
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 3 cut(s) 89, 230, 474
BstKTI GATC 3 cut(s) 210, 578, 1096
BstMAI GTCTC 4 cut(s) 307, 525, 760, 788
BstMBI GATC 3 cut(s) 207, 575, 1093
BstMWI GCNNNNNNNGC 8 cut(s) 84, 95, 161, 346, 471, 907, 1283, 1355
BstNI CCWGG 2 cut(s) 311, 448
BstSCI CCNGG 2 cut(s) 309, 446
BstSFI CTRYAG 2 cut(s) 1014, 1284
BstSLI GKGCMC 3 cut(s) 266, 612, 1089
BstV1I GCAGC 3 cut(s) 715, 1261, 1298
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BsuRI GGCC 2 cut(s) 98, 910
BtgI CCRYGG 1 cut(s) 984
BtsCI GGATG 1 cut(s) 24
BtsI GCAGTG 2 cut(s) 1183, 1344
BtsIMutI CAGTG 6 cut(s) 264, 540, 888, 904, 1183, 1344
Cac8I GCNNGC 2 cut(s) 384, 553
CfoI GCGC 3 cut(s) 89, 230, 474
Cfr13I GGNCC 2 cut(s) 360, 1033
CseI GACGC 1 cut(s) 1112
Csp6I GTAC 4 cut(s) 116, 314, 408, 1065
CviAII CATG 6 cut(s) 43, 475, 770, 805, 811, 985
CviQI GTAC 4 cut(s) 116, 314, 408, 1065
DdeI CTNAG 2 cut(s) 267, 350
DinI GGCGCC 1 cut(s) 229
DpnI GATC 3 cut(s) 209, 577, 1095
DpnII GATC 3 cut(s) 207, 575, 1093
DraI TTTAAA 1 cut(s) 336
EaeI YGGCCR 1 cut(s) 908
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 341, 984
Eco31I GGTCTC 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 360, 1033
EcoO109I RGGNCCY 2 cut(s) 360, 1033
EcoRII CCWGG 2 cut(s) 309, 446
EcoT14I CCWWGG 2 cut(s) 341, 984
EgeI GGCGCC 1 cut(s) 229
EheI GGCGCC 1 cut(s) 229
ErhI CCWWGG 2 cut(s) 341, 984
Esp3I CGTCTC 1 cut(s) 525
FaeI CATG 6 cut(s) 46, 478, 773, 808, 814, 988
FalI AAGNNNNNCTT 2 cut(s) 113, 145
FaqI GGGAC 3 cut(s) 334, 346, 964
FatI CATG 6 cut(s) 42, 474, 769, 804, 810, 984
FauNDI CATATG 1 cut(s) 91
FbaI TGATCA 1 cut(s) 207
Fnu4HI GCNGC 3 cut(s) 729, 1275, 1287
FokI GGATG 1 cut(s) 31
Fsp4HI GCNGC 3 cut(s) 729, 1275, 1287
FspI TGCGCA 1 cut(s) 473
GlaI GCGC 3 cut(s) 88, 229, 473
GluI GCNGC 3 cut(s) 729, 1275, 1287
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 2 cut(s) 98, 910
HgaI GACGC 1 cut(s) 1112
HhaI GCGC 3 cut(s) 89, 230, 474
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 6 cut(s) 46, 478, 773, 808, 814, 988
Hin6I GCGC 3 cut(s) 87, 228, 472
HinP1I GCGC 3 cut(s) 87, 228, 472
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 4 cut(s) 124, 478, 562, 709
HphI GGTGA 2 cut(s) 436, 1091
Hpy166II GTNNAC 4 cut(s) 48, 419, 776, 1237
Hpy188I TCNGA 3 cut(s) 194, 461, 865
Hpy188III TCNNGA 2 cut(s) 1097, 1154
Hpy8I GTNNAC 4 cut(s) 48, 419, 776, 1237
HpyAV CCTTC 2 cut(s) 218, 741
HpyCH4III ACNGT 3 cut(s) 799, 831, 1234
HpyCH4IV ACGT 1 cut(s) 415
HpyCH4V TGCA 9 cut(s) 22, 105, 188, 386, 731, 873, 1277, 1286, 1349
HpyF10VI GCNNNNNNNGC 8 cut(s) 84, 95, 161, 346, 471, 907, 1283, 1355
HpyF3I CTNAG 2 cut(s) 267, 350
HpySE526I ACGT 1 cut(s) 415
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 6 cut(s) 46, 478, 773, 808, 814, 988
HspAI GCGC 3 cut(s) 87, 228, 472
KasI GGCGCC 1 cut(s) 227
KflI GGGWCCC 1 cut(s) 360
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 3 cut(s) 207, 575, 1093
LguI GCTCTTC 1 cut(s) 192
LmnI GCTCC 1 cut(s) 161
Lsp1109I GCAGC 3 cut(s) 715, 1261, 1298
LweI GCATC 3 cut(s) 9, 474, 1138
MaeII ACGT 1 cut(s) 415
MalI GATC 3 cut(s) 209, 577, 1095
MboI GATC 3 cut(s) 207, 575, 1093
MboII GAAGA 3 cut(s) 209, 464, 827
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 3 cut(s) 266, 612, 1089
MluCI AATT 8 cut(s) 294, 390, 504, 678, 955, 1255, 1291, 1340
Mly113I GGCGCC 1 cut(s) 228
MlyI GAGTC 1 cut(s) 487
MmeI TCCRAC 2 cut(s) 553, 823
MnlI CCTC 7 cut(s) 88, 175, 839, 1024, 1075, 1150, 1323
MseI TTAA 6 cut(s) 143, 335, 581, 762, 780, 975
MslI CAYNNNNRTG 2 cut(s) 809, 893
MspA1I CMGCKG 1 cut(s) 728
MspR9I CCNGG 2 cut(s) 311, 448
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 188
MvaI CCWGG 2 cut(s) 311, 448
MwoI GCNNNNNNNGC 8 cut(s) 84, 95, 161, 346, 471, 907, 1283, 1355
NarI GGCGCC 1 cut(s) 228
NcoI CCATGG 1 cut(s) 984
NdeI CATATG 1 cut(s) 91
NdeII GATC 3 cut(s) 207, 575, 1093
NlaIII CATG 6 cut(s) 46, 478, 773, 808, 814, 988
NlaIV GGNNCC 7 cut(s) 163, 229, 361, 362, 577, 1035, 1223
NmeAIII GCCGAG 1 cut(s) 124
NsbI TGCGCA 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 893
PciSI GCTCTTC 1 cut(s) 192
PctI GAATGC 1 cut(s) 188
PfeI GAWTC 3 cut(s) 124, 562, 709
PflFI GACNNNGTC 1 cut(s) 43
PflMI CCANNNNNTGG 2 cut(s) 680, 816
PkrI GCNGC 3 cut(s) 730, 1276, 1288
PleI GAGTC 1 cut(s) 486
PluTI GGCGCC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 486
PpuMI RGGWCCY 2 cut(s) 360, 1033
Psp5II RGGWCCY 2 cut(s) 360, 1033
Psp6I CCWGG 2 cut(s) 309, 446
PspGI CCWGG 2 cut(s) 309, 446
PspN4I GGNNCC 7 cut(s) 163, 229, 361, 362, 577, 1035, 1223
PspPI GGNCC 2 cut(s) 360, 1033
PspPPI RGGWCCY 2 cut(s) 360, 1033
PstI CTGCAG 1 cut(s) 1288
PsuI RGATCY 1 cut(s) 575
PsyI GACNNNGTC 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 728
RsaI GTAC 4 cut(s) 117, 315, 409, 1066
RsaNI GTAC 4 cut(s) 116, 314, 408, 1065
RseI CAYNNNNRTG 2 cut(s) 809, 893
SapI GCTCTTC 1 cut(s) 192
SaqAI TTAA 6 cut(s) 143, 335, 581, 762, 780, 975
SatI GCNGC 3 cut(s) 729, 1275, 1287
Sau3AI GATC 3 cut(s) 207, 575, 1093
Sau96I GGNCC 2 cut(s) 360, 1033
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 2 cut(s) 311, 448
SduI GDGCHC 3 cut(s) 266, 612, 1089
SfaNI GCATC 3 cut(s) 9, 474, 1138
SfcI CTRYAG 2 cut(s) 1014, 1284
SfoI GGCGCC 1 cut(s) 229
SinI GGWCC 2 cut(s) 360, 1033
SmiMI CAYNNNNRTG 2 cut(s) 809, 893
SmlI CTYRAG 2 cut(s) 1007, 1154
SmoI CTYRAG 2 cut(s) 1007, 1154
Sse9I AATT 8 cut(s) 294, 390, 504, 678, 955, 1255, 1291, 1340
SspDI GGCGCC 1 cut(s) 227
SspI AATATT 2 cut(s) 650, 1317
StyD4I CCNGG 2 cut(s) 309, 446
StyI CCWWGG 2 cut(s) 341, 984
TaaI ACNGT 3 cut(s) 799, 831, 1234
TaiI ACGT 1 cut(s) 418
TaqI TCGA 2 cut(s) 695, 930
TasI AATT 8 cut(s) 294, 390, 504, 678, 955, 1255, 1291, 1340
TatI WGTACW 2 cut(s) 115, 407
TfiI GAWTC 3 cut(s) 124, 562, 709
Tru1I TTAA 6 cut(s) 143, 335, 581, 762, 780, 975
Tru9I TTAA 6 cut(s) 143, 335, 581, 762, 780, 975
TscAI CASTG 6 cut(s) 264, 547, 895, 911, 1183, 1351
TseI GCWGC 3 cut(s) 728, 1274, 1286
TspDTI ATGAA 3 cut(s) 254, 575, 678
TspGWI ACGGA 1 cut(s) 1082
TspRI CASTG 6 cut(s) 264, 547, 895, 911, 1183, 1351
Tth111I GACNNNGTC 1 cut(s) 43
Van91I CCANNNNNTGG 2 cut(s) 680, 816
VpaK11BI GGWCC 2 cut(s) 360, 1033
XapI RAATTY 2 cut(s) 294, 1340
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.