Rorug05G0338900

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001179
Physical Location & Seq
Reverse (-)
41154878 .. 41155877
1000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0338900.1

Sequence Viewer

Length: 723 bp
ATGGCAGACCCTTCTCGGCCCGTCACCGGCTACCCAGCCCCGCCCTACGCCCAGCCCTACCCCGCCAACAACGGCCAGCCCAACGGCTCCTACCCCAACTACCCAGTCTACCAGCGCGGCGGCCCCTACAACGCGTACACCGCACGCTCCGCCTTCCTCCGCCGCTTCGTCATCGCCATGGTCTTCCTCTTCGTCATCATCGGCTCCCTCCTCCTCATGATCTGGCTCATCCTCCGCCCCAAGATCCCCGACTTCCGAGTCGACTCCTTCACCGTCACCAACCTCAACGTCTCCTCCTCCCGCCAGTCCCTCACCGGCACCTGGTCCGTCGGCTTCGACGTCTTCAACCCCAACAAGAAGATGACCATCGAGTACGACGAGATCGGCTCCTCCGTCTTCTACCGCTCCGGCTTCATTTCCGAGACCCGAATCCCGCCGTTCCGGCAGGGAAAGAGAGTCCGCAGCGCCGTGAACGCGACTTTCTCGGCGGCGAACTCGTTCGTCGACGATAGGGTGGCGAGTGGGATCGACGCGGACAGGGCGGCGCGTGGGGCGGTGAGTTTCAATATCAAGCTGCTGGCTAGGGTTCAGTTTCGGAAGGGCGGGTGGAGGCTGAGGCGGCGGCTGCTGAGGGTGCTGTGCCGTGACGTGCCGCTTACGATTTCGAATAATGGGACCGGGACGATGACCGGCGGAGGTAGAGATTGCGGCGTTGCTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

240

Amino Acids

26.59

Weight (kDa)

10.64

Isoelectric Point (pI)

49.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AatII GACGTC 1 cut(s) 342
AccB1I GGYRCC 1 cut(s) 317
AccBSI CCGCTC 1 cut(s) 405
AccI GTMKAC 3 cut(s) 108, 261, 504
AccII CGCG 5 cut(s) 117, 134, 476, 533, 547
AclWI GGATC 2 cut(s) 238, 533
AcoI YGGCCR 1 cut(s) 73
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 2 cut(s) 137, 374
AfiI CCNNNNNNNGG 2 cut(s) 26, 321
AflIII ACRYGT 1 cut(s) 132
AgsI TTSAA 2 cut(s) 346, 565
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 1 cut(s) 574
AluI AGCT 1 cut(s) 574
Alw26I GTCTC 2 cut(s) 295, 416
AlwI GGATC 2 cut(s) 238, 533
AoxI GGCC 3 cut(s) 17, 73, 121
ApeKI GCWGC 3 cut(s) 462, 574, 625
Asp700I GAANNNNTTC 1 cut(s) 497
AspLEI GCGC 3 cut(s) 117, 467, 547
AspS9I GGNCC 4 cut(s) 18, 122, 324, 675
AsuC2I CCSGG 1 cut(s) 679
AsuHPI GGTGA 5 cut(s) 16, 262, 268, 304, 568
AsuII TTCGAA 1 cut(s) 665
AvaII GGWCC 2 cut(s) 324, 675
BanI GGYRCC 1 cut(s) 317
BbsI GAAGAC 3 cut(s) 175, 334, 388
BbvCI CCTCAGC 2 cut(s) 614, 629
BbvI GCAGC 3 cut(s) 474, 561, 612
BccI CCATC 1 cut(s) 374
BceAI ACGGC 5 cut(s) 88, 100, 421, 452, 627
BciT130I CCWGG 1 cut(s) 322
BcnI CCSGG 1 cut(s) 679
BcoDI GTCTC 2 cut(s) 295, 416
BfaI CTAG 1 cut(s) 582
BfoI RGCGCY 1 cut(s) 468
BglI GCCNNNNNGGC 1 cut(s) 442
Bme1390I CCNGG 2 cut(s) 322, 679
Bme18I GGWCC 2 cut(s) 324, 675
BmgBI CACGTC 1 cut(s) 649
BmgT120I GGNCC 4 cut(s) 18, 122, 324, 675
BmiI GGNNCC 6 cut(s) 88, 124, 205, 319, 388, 676
BmrFI CCNGG 2 cut(s) 322, 679
BmrI ACTGGG 1 cut(s) 98
BmuI ACTGGG 1 cut(s) 98
BpiI GAAGAC 3 cut(s) 175, 334, 388
BplI GAGNNNNNCTC 2 cut(s) 371, 403
Bpu10I CCTNAGC 2 cut(s) 614, 629
Bpu14I TTCGAA 1 cut(s) 665
BpuMI CCSGG 1 cut(s) 679
BsaBI GATNNNNATC 1 cut(s) 365
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 416
BsaJI CCNNGG 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 321
Bse118I RCCGGY 3 cut(s) 26, 314, 689
Bse1I ACTGG 2 cut(s) 104, 304
Bse8I GATNNNNATC 1 cut(s) 365
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 1 cut(s) 228
BseJI GATNNNNATC 1 cut(s) 365
BseLI CCNNNNNNNGG 2 cut(s) 26, 321
BseMII CTCAG 2 cut(s) 605, 620
BseNI ACTGG 2 cut(s) 104, 304
BseRI GAGGAG 5 cut(s) 200, 203, 283, 286, 379
BseXI GCAGC 3 cut(s) 474, 561, 612
BseYI CCCAGC 2 cut(s) 34, 51
Bsh1236I CGCG 5 cut(s) 117, 134, 476, 533, 547
BshFI GGCC 3 cut(s) 19, 75, 123
BshNI GGYRCC 1 cut(s) 317
BsiSI CCGG 6 cut(s) 27, 315, 408, 442, 678, 690
BslFI GGGAC 3 cut(s) 292, 688, 694
BslI CCNNNNNNNGG 2 cut(s) 26, 321
BsmAI GTCTC 2 cut(s) 295, 416
BsmBI CGTCTC 1 cut(s) 295
BsmFI GGGAC 3 cut(s) 292, 688, 694
BsnI GGCC 3 cut(s) 19, 75, 123
Bso31I GGTCTC 1 cut(s) 416
Bsp119I TTCGAA 1 cut(s) 665
Bsp143I GATC 4 cut(s) 219, 243, 381, 525
Bsp19I CCATGG 1 cut(s) 177
BspANI GGCC 3 cut(s) 19, 75, 123
BspCNI CTCAG 2 cut(s) 606, 621
BspFNI CGCG 5 cut(s) 117, 134, 476, 533, 547
BspHI TCATGA 1 cut(s) 216
BspLI GGNNCC 6 cut(s) 88, 124, 205, 319, 388, 676
BspPI GGATC 2 cut(s) 238, 533
BspT104I TTCGAA 1 cut(s) 665
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 416
BsrBI CCGCTC 1 cut(s) 405
BsrFI RCCGGY 3 cut(s) 26, 314, 689
BsrI ACTGG 2 cut(s) 104, 304
BssAI RCCGGY 3 cut(s) 26, 314, 689
BssECI CCNNGG 1 cut(s) 177
BssMI GATC 4 cut(s) 219, 243, 381, 525
BssNI GRCGYC 1 cut(s) 339
BssT1I CCWWGG 1 cut(s) 177
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 1 cut(s) 194
BstACI GRCGYC 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 77, 145, 579
BstDEI CTNAG 2 cut(s) 614, 629
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 5 cut(s) 117, 134, 476, 533, 547
BstH2I RGCGCY 1 cut(s) 468
BstHHI GCGC 3 cut(s) 117, 467, 547
BstKTI GATC 4 cut(s) 222, 246, 384, 528
BstMAI GTCTC 2 cut(s) 295, 416
BstMBI GATC 4 cut(s) 219, 243, 381, 525
BstMWI GCNNNNNNNGC 9 cut(s) 140, 149, 442, 473, 539, 551, 619, 625, 634
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 2 cut(s) 320, 677
BstUI CGCG 5 cut(s) 117, 134, 476, 533, 547
BstV1I GCAGC 3 cut(s) 474, 561, 612
BstV2I GAAGAC 3 cut(s) 175, 334, 388
BstX2I RGATCY 1 cut(s) 243
BstYI RGATCY 1 cut(s) 243
BsuRI GGCC 3 cut(s) 19, 75, 123
BtgI CCRYGG 1 cut(s) 177
BtgZI GCGATG 1 cut(s) 157
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 1 cut(s) 228
Cac8I GCNNGC 3 cut(s) 77, 145, 579
CciI TCATGA 1 cut(s) 216
CfoI GCGC 3 cut(s) 117, 467, 547
Cfr10I RCCGGY 3 cut(s) 26, 314, 689
Cfr13I GGNCC 4 cut(s) 18, 122, 324, 675
CseI GACGC 1 cut(s) 539
CsiI ACCWGGT 1 cut(s) 320
Csp6I GTAC 2 cut(s) 136, 373
CviAII CATG 2 cut(s) 178, 217
CviQI GTAC 2 cut(s) 136, 373
DdeI CTNAG 2 cut(s) 614, 629
DpnI GATC 4 cut(s) 221, 245, 383, 527
DpnII GATC 4 cut(s) 219, 243, 381, 525
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EciI GGCGGA 4 cut(s) 139, 149, 224, 708
Eco130I CCWWGG 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 416
Eco47I GGWCC 2 cut(s) 324, 675
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 177
Esp3I CGTCTC 1 cut(s) 295
FaeI CATG 2 cut(s) 181, 220
FaiI YATR 3 cut(s) 179, 218, 721
FaqI GGGAC 3 cut(s) 292, 688, 694
FatI CATG 2 cut(s) 177, 216
FauI CCCGC 5 cut(s) 48, 70, 308, 441, 596
FblI GTMKAC 3 cut(s) 108, 261, 504
FokI GGATG 1 cut(s) 215
FspBI CTAG 1 cut(s) 582
GlaI GCGC 3 cut(s) 116, 466, 546
GsaI CCCAGC 2 cut(s) 38, 55
HaeII RGCGCY 1 cut(s) 468
HaeIII GGCC 3 cut(s) 19, 75, 123
HapII CCGG 6 cut(s) 27, 315, 408, 442, 678, 690
HgaI GACGC 1 cut(s) 539
HhaI GCGC 3 cut(s) 117, 467, 547
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 2 cut(s) 181, 220
Hin6I GCGC 3 cut(s) 115, 465, 545
HinP1I GCGC 3 cut(s) 115, 465, 545
HincII GTYRAC 2 cut(s) 262, 505
HindII GTYRAC 2 cut(s) 262, 505
HinfI GANTC 4 cut(s) 258, 263, 429, 456
HpaII CCGG 6 cut(s) 27, 315, 408, 442, 678, 690
HphI GGTGA 5 cut(s) 16, 262, 268, 304, 568
Hpy166II GTNNAC 5 cut(s) 109, 138, 262, 472, 505
Hpy188I TCNGA 3 cut(s) 257, 421, 597
Hpy188III TCNNGA 1 cut(s) 217
Hpy8I GTNNAC 5 cut(s) 109, 138, 262, 472, 505
Hpy99I CGWCG 6 cut(s) 332, 341, 380, 506, 509, 533
HpyAV CCTTC 4 cut(s) 21, 163, 277, 592
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 3 cut(s) 288, 339, 648
HpyF10VI GCNNNNNNNGC 9 cut(s) 140, 149, 442, 473, 539, 551, 619, 625, 634
HpyF3I CTNAG 2 cut(s) 614, 629
HpySE526I ACGT 3 cut(s) 288, 339, 648
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 2 cut(s) 181, 220
HspAI GCGC 3 cut(s) 115, 465, 545
Kzo9I GATC 4 cut(s) 219, 243, 381, 525
LmnI GCTCC 5 cut(s) 92, 152, 209, 392, 410
Lsp1109I GCAGC 3 cut(s) 474, 561, 612
MabI ACCWGGT 1 cut(s) 320
MaeI CTAG 1 cut(s) 582
MaeII ACGT 3 cut(s) 288, 339, 648
MaeIII GTNAC 3 cut(s) 22, 274, 644
MalI GATC 4 cut(s) 221, 245, 383, 527
MbiI CCGCTC 1 cut(s) 405
MboI GATC 4 cut(s) 219, 243, 381, 525
MboII GAAGA 5 cut(s) 175, 181, 334, 370, 388
MflI RGATCY 1 cut(s) 243
MluI ACGCGT 1 cut(s) 132
MlyI GAGTC 3 cut(s) 257, 267, 465
MroXI GAANNNNTTC 1 cut(s) 497
MslI CAYNNNNRTG 1 cut(s) 176
MspI CCGG 6 cut(s) 27, 315, 408, 442, 678, 690
MspR9I CCNGG 2 cut(s) 322, 679
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 5 cut(s) 117, 134, 476, 533, 547
MwoI GCNNNNNNNGC 9 cut(s) 140, 149, 442, 473, 539, 551, 619, 625, 634
NciI CCSGG 1 cut(s) 679
NcoI CCATGG 1 cut(s) 177
NdeII GATC 4 cut(s) 219, 243, 381, 525
NlaIII CATG 2 cut(s) 181, 220
NlaIV GGNNCC 6 cut(s) 88, 124, 205, 319, 388, 676
NmeAIII GCCGAG 1 cut(s) 464
NmuCI GTSAC 3 cut(s) 22, 274, 644
NspV TTCGAA 1 cut(s) 665
PagI TCATGA 1 cut(s) 216
PcsI WCGNNNNNNNCGW 3 cut(s) 336, 375, 390
PdmI GAANNNNTTC 1 cut(s) 497
PfeI GAWTC 1 cut(s) 429
PleI GAGTC 3 cut(s) 257, 266, 464
PpsI GAGTC 3 cut(s) 257, 266, 464
Psp6I CCWGG 1 cut(s) 320
PspFI CCCAGC 2 cut(s) 34, 51
PspGI CCWGG 1 cut(s) 320
PspN4I GGNNCC 6 cut(s) 88, 124, 205, 319, 388, 676
PspPI GGNCC 4 cut(s) 18, 122, 324, 675
PsuI RGATCY 1 cut(s) 243
RsaI GTAC 2 cut(s) 137, 374
RsaNI GTAC 2 cut(s) 136, 373
RseI CAYNNNNRTG 1 cut(s) 176
SalI GTCGAC 2 cut(s) 260, 503
Sau3AI GATC 4 cut(s) 219, 243, 381, 525
Sau96I GGNCC 4 cut(s) 18, 122, 324, 675
SchI GAGTC 3 cut(s) 257, 267, 465
ScrFI CCNGG 2 cut(s) 322, 679
SetI ASST 7 cut(s) 285, 291, 323, 342, 576, 651, 700
SexAI ACCWGGT 1 cut(s) 320
SfuI TTCGAA 1 cut(s) 665
SgrDI CGTCGACG 1 cut(s) 503
SinI GGWCC 2 cut(s) 324, 675
SmiMI CAYNNNNRTG 1 cut(s) 176
SspMI CTAG 1 cut(s) 582
StyD4I CCNGG 2 cut(s) 320, 677
StyI CCWWGG 1 cut(s) 177
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 3 cut(s) 291, 342, 651
TaqI TCGA 6 cut(s) 261, 336, 369, 504, 528, 665
TauI GCSGC 9 cut(s) 120, 123, 165, 491, 545, 622, 625, 655, 711
TfiI GAWTC 1 cut(s) 429
TseFI GTSAC 3 cut(s) 22, 274, 644
TseI GCWGC 3 cut(s) 462, 574, 625
Tsp45I GTSAC 3 cut(s) 22, 274, 644
TspDTI ATGAA 1 cut(s) 403
TspGWI ACGGA 2 cut(s) 316, 382
VpaK11BI GGWCC 2 cut(s) 324, 675
XmiI GTMKAC 3 cut(s) 108, 261, 504
XmnI GAANNNNTTC 1 cut(s) 497
XspI CTAG 1 cut(s) 582
ZraI GACGTC 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.