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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 145, 350 |
| AclWI | GGATC | 2 cut(s) | 90, 295 |
| AgsI | TTSAA | 1 cut(s) | 17 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 262, 294 |
| Alw26I | GTCTC | 1 cut(s) | 311 |
| AlwI | GGATC | 2 cut(s) | 90, 295 |
| ApeKI | GCWGC | 1 cut(s) | 179 |
| AsuHPI | GGTGA | 1 cut(s) | 272 |
| BbvI | GCAGC | 1 cut(s) | 191 |
| BccI | CCATC | 2 cut(s) | 17, 219 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 171, 205 |
| BcoDI | GTCTC | 1 cut(s) | 311 |
| BisI | GCNGC | 7 cut(s) | 102, 180, 240, 243, 246, 249, 307 |
| BlsI | GCNGC | 7 cut(s) | 103, 181, 241, 244, 247, 250, 308 |
| BpuEI | CTTGAG | 2 cut(s) | 151, 356 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 80, 110, 285, 315 |
| BseGI | GGATG | 2 cut(s) | 28, 230 |
| BseRI | GAGGAG | 1 cut(s) | 391 |
| BseXI | GCAGC | 1 cut(s) | 191 |
| Bsh1236I | CGCG | 2 cut(s) | 145, 350 |
| Bsh1285I | CGRYCG | 1 cut(s) | 354 |
| BsiEI | CGRYCG | 1 cut(s) | 354 |
| BslFI | GGGAC | 2 cut(s) | 140, 345 |
| BsmAI | GTCTC | 1 cut(s) | 311 |
| BsmFI | GGGAC | 2 cut(s) | 140, 345 |
| Bsp143I | GATC | 2 cut(s) | 82, 287 |
| BspFNI | CGCG | 2 cut(s) | 145, 350 |
| BspPI | GGATC | 2 cut(s) | 90, 295 |
| BssMI | GATC | 2 cut(s) | 82, 287 |
| Bst6I | CTCTTC | 1 cut(s) | 12 |
| BstC8I | GCNNGC | 1 cut(s) | 184 |
| BstF5I | GGATG | 2 cut(s) | 28, 230 |
| BstFNI | CGCG | 2 cut(s) | 145, 350 |
| BstKTI | GATC | 2 cut(s) | 85, 290 |
| BstMAI | GTCTC | 1 cut(s) | 311 |
| BstMBI | GATC | 2 cut(s) | 82, 287 |
| BstMCI | CGRYCG | 1 cut(s) | 354 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 245, 248 |
| BstNSI | RCATGY | 1 cut(s) | 186 |
| BstUI | CGCG | 2 cut(s) | 145, 350 |
| BstV1I | GCAGC | 1 cut(s) | 191 |
| BtsCI | GGATG | 2 cut(s) | 28, 230 |
| Cac8I | GCNNGC | 1 cut(s) | 184 |
| CviAII | CATG | 1 cut(s) | 183 |
| DpnI | GATC | 2 cut(s) | 84, 289 |
| DpnII | GATC | 2 cut(s) | 82, 287 |
| Eam1104I | CTCTTC | 1 cut(s) | 12 |
| EarI | CTCTTC | 1 cut(s) | 12 |
| Eco32I | GATATC | 1 cut(s) | 386 |
| EcoRV | GATATC | 1 cut(s) | 386 |
| FaeI | CATG | 1 cut(s) | 186 |
| FaiI | YATR | 2 cut(s) | 59, 184 |
| FaqI | GGGAC | 2 cut(s) | 140, 345 |
| FatI | CATG | 1 cut(s) | 182 |
| FauI | CCCGC | 2 cut(s) | 33, 356 |
| Fnu4HI | GCNGC | 7 cut(s) | 102, 180, 240, 243, 246, 249, 307 |
| FokI | GGATG | 2 cut(s) | 35, 237 |
| Fsp4HI | GCNGC | 7 cut(s) | 102, 180, 240, 243, 246, 249, 307 |
| GluI | GCNGC | 7 cut(s) | 102, 180, 240, 243, 246, 249, 307 |
| Hin1II | CATG | 1 cut(s) | 186 |
| HphI | GGTGA | 1 cut(s) | 272 |
| Hpy166II | GTNNAC | 1 cut(s) | 8 |
| Hpy188III | TCNNGA | 3 cut(s) | 86, 291, 316 |
| Hpy8I | GTNNAC | 1 cut(s) | 8 |
| HpyAV | CCTTC | 2 cut(s) | 353, 364 |
| HpyCH4V | TGCA | 2 cut(s) | 179, 186 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 245, 248 |
| Hsp92II | CATG | 1 cut(s) | 186 |
| Kzo9I | GATC | 2 cut(s) | 82, 287 |
| LpnPI | CCDG | 4 cut(s) | 64, 101, 269, 306 |
| Lsp1109I | GCAGC | 1 cut(s) | 191 |
| MalI | GATC | 2 cut(s) | 84, 289 |
| MboI | GATC | 2 cut(s) | 82, 287 |
| MboII | GAAGA | 2 cut(s) | 29, 181 |
| MluCI | AATT | 1 cut(s) | 12 |
| MvnI | CGCG | 2 cut(s) | 145, 350 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 245, 248 |
| NdeII | GATC | 2 cut(s) | 82, 287 |
| NlaIII | CATG | 1 cut(s) | 186 |
| NspI | RCATGY | 1 cut(s) | 186 |
| PaeI | GCATGC | 1 cut(s) | 186 |
| PkrI | GCNGC | 7 cut(s) | 103, 181, 241, 244, 247, 250, 308 |
| SatI | GCNGC | 7 cut(s) | 102, 180, 240, 243, 246, 249, 307 |
| Sau3AI | GATC | 2 cut(s) | 82, 287 |
| SetI | ASST | 2 cut(s) | 110, 315 |
| SmlI | CTYRAG | 2 cut(s) | 130, 335 |
| SmoI | CTYRAG | 2 cut(s) | 130, 335 |
| SphI | GCATGC | 1 cut(s) | 186 |
| Sse9I | AATT | 1 cut(s) | 12 |
| SspI | AATATT | 1 cut(s) | 175 |
| TaqI | TCGA | 4 cut(s) | 110, 191, 315, 354 |
| TasI | AATT | 1 cut(s) | 12 |
| TauI | GCSGC | 6 cut(s) | 104, 242, 245, 248, 251, 309 |
| TseI | GCWGC | 1 cut(s) | 179 |
| TspDTI | ATGAA | 1 cut(s) | 372 |
| XceI | RCATGY | 1 cut(s) | 186 |