POT family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 125 |
| AciI | CCGC | 2 cut(s) | 90, 148 |
| AcoI | YGGCCR | 1 cut(s) | 165 |
| AcsI | RAATTY | 2 cut(s) | 447, 493 |
| AfaI | GTAC | 3 cut(s) | 59, 134, 338 |
| AflII | CTTAAG | 1 cut(s) | 367 |
| AgsI | TTSAA | 2 cut(s) | 97, 288 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 105, 137 |
| AluBI | AGCT | 1 cut(s) | 372 |
| AluI | AGCT | 1 cut(s) | 372 |
| Alw21I | GWGCWC | 1 cut(s) | 298 |
| Alw44I | GTGCAC | 1 cut(s) | 294 |
| AoxI | GGCC | 1 cut(s) | 165 |
| ApaLI | GTGCAC | 1 cut(s) | 294 |
| ApoI | RAATTY | 2 cut(s) | 447, 493 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 21 |
| AsuHPI | GGTGA | 2 cut(s) | 122, 233 |
| AsuNHI | GCTAGC | 1 cut(s) | 86 |
| BaeGI | GKGCMC | 1 cut(s) | 298 |
| BalI | TGGCCA | 1 cut(s) | 167 |
| BanI | GGYRCC | 1 cut(s) | 125 |
| BauI | CACGAG | 1 cut(s) | 408 |
| BbsI | GAAGAC | 1 cut(s) | 24 |
| Bbv12I | GWGCWC | 1 cut(s) | 298 |
| BccI | CCATC | 3 cut(s) | 76, 176, 435 |
| BfaI | CTAG | 2 cut(s) | 87, 161 |
| BfmI | CTRYAG | 1 cut(s) | 26 |
| BfrI | CTTAAG | 1 cut(s) | 367 |
| BmiI | GGNNCC | 1 cut(s) | 127 |
| BmtI | GCTAGC | 1 cut(s) | 90 |
| BpiI | GAAGAC | 1 cut(s) | 24 |
| BpuEI | CTTGAG | 1 cut(s) | 449 |
| BsaJI | CCNNGG | 1 cut(s) | 504 |
| Bse1I | ACTGG | 1 cut(s) | 50 |
| BseDI | CCNNGG | 1 cut(s) | 504 |
| BseGI | GGATG | 1 cut(s) | 440 |
| BseMII | CTCAG | 2 cut(s) | 81, 362 |
| BseNI | ACTGG | 1 cut(s) | 50 |
| BseSI | GKGCMC | 1 cut(s) | 298 |
| BshFI | GGCC | 1 cut(s) | 167 |
| BshNI | GGYRCC | 1 cut(s) | 125 |
| BsiHKAI | GWGCWC | 1 cut(s) | 298 |
| BsnI | GGCC | 1 cut(s) | 167 |
| Bsp1286I | GDGCHC | 1 cut(s) | 298 |
| Bsp1407I | TGTACA | 1 cut(s) | 132 |
| BspACI | CCGC | 2 cut(s) | 90, 148 |
| BspANI | GGCC | 1 cut(s) | 167 |
| BspCNI | CTCAG | 2 cut(s) | 80, 361 |
| BspLI | GGNNCC | 1 cut(s) | 127 |
| BspMAI | CTGCAG | 1 cut(s) | 30 |
| BspOI | GCTAGC | 1 cut(s) | 90 |
| BspT107I | GGYRCC | 1 cut(s) | 125 |
| BspTI | CTTAAG | 1 cut(s) | 367 |
| BsrGI | TGTACA | 1 cut(s) | 132 |
| BsrI | ACTGG | 1 cut(s) | 50 |
| BssECI | CCNNGG | 1 cut(s) | 504 |
| BssSI | CACGAG | 1 cut(s) | 408 |
| BssT1I | CCWWGG | 1 cut(s) | 504 |
| Bst2BI | CACGAG | 1 cut(s) | 408 |
| Bst4CI | ACNGT | 3 cut(s) | 130, 284, 293 |
| BstAFI | CTTAAG | 1 cut(s) | 367 |
| BstAUI | TGTACA | 1 cut(s) | 132 |
| BstC8I | GCNNGC | 1 cut(s) | 88 |
| BstDEI | CTNAG | 2 cut(s) | 67, 348 |
| BstF5I | GGATG | 1 cut(s) | 440 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 156 |
| BstNSI | RCATGY | 1 cut(s) | 445 |
| BstSFI | CTRYAG | 1 cut(s) | 26 |
| BstSLI | GKGCMC | 1 cut(s) | 298 |
| BstV2I | GAAGAC | 1 cut(s) | 24 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 546 |
| BsuRI | GGCC | 1 cut(s) | 167 |
| BtsCI | GGATG | 1 cut(s) | 440 |
| BtsI | GCAGTG | 1 cut(s) | 157 |
| BtsIMutI | CAGTG | 1 cut(s) | 157 |
| Cac8I | GCNNGC | 1 cut(s) | 88 |
| Csp6I | GTAC | 3 cut(s) | 58, 133, 337 |
| CviAII | CATG | 5 cut(s) | 46, 245, 313, 442, 540 |
| CviJI | RGCY | 9 cut(s) | 86, 120, 167, 270, 324, 372, 407, 469, 532 |
| CviKI_1 | RGCY | 9 cut(s) | 86, 120, 167, 270, 324, 372, 407, 469, 532 |
| CviQI | GTAC | 3 cut(s) | 58, 133, 337 |
| DdeI | CTNAG | 2 cut(s) | 67, 348 |
| EaeI | YGGCCR | 1 cut(s) | 165 |
| Eco130I | CCWWGG | 1 cut(s) | 504 |
| EcoT14I | CCWWGG | 1 cut(s) | 504 |
| ErhI | CCWWGG | 1 cut(s) | 504 |
| FaeI | CATG | 5 cut(s) | 49, 248, 316, 445, 543 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 46, 78 |
| FatI | CATG | 5 cut(s) | 45, 244, 312, 441, 539 |
| FokI | GGATG | 1 cut(s) | 447 |
| FspBI | CTAG | 2 cut(s) | 87, 161 |
| HaeIII | GGCC | 1 cut(s) | 167 |
| Hin1II | CATG | 5 cut(s) | 49, 248, 316, 445, 543 |
| HincII | GTYRAC | 2 cut(s) | 250, 280 |
| HindII | GTYRAC | 2 cut(s) | 250, 280 |
| HinfI | GANTC | 3 cut(s) | 93, 238, 414 |
| HpaI | GTTAAC | 1 cut(s) | 250 |
| HphI | GGTGA | 2 cut(s) | 122, 233 |
| Hpy166II | GTNNAC | 4 cut(s) | 133, 250, 280, 296 |
| Hpy188I | TCNGA | 4 cut(s) | 70, 233, 460, 514 |
| Hpy188III | TCNNGA | 3 cut(s) | 172, 329, 485 |
| Hpy8I | GTNNAC | 4 cut(s) | 133, 250, 280, 296 |
| HpyAV | CCTTC | 2 cut(s) | 107, 228 |
| HpyCH4III | ACNGT | 3 cut(s) | 130, 284, 293 |
| HpyCH4IV | ACGT | 1 cut(s) | 252 |
| HpyCH4V | TGCA | 3 cut(s) | 28, 296, 395 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 156 |
| HpyF3I | CTNAG | 2 cut(s) | 67, 348 |
| HpySE526I | ACGT | 1 cut(s) | 252 |
| Hsp92II | CATG | 5 cut(s) | 49, 248, 316, 445, 543 |
| KspAI | GTTAAC | 1 cut(s) | 250 |
| LpnPI | CCDG | 1 cut(s) | 63 |
| MaeI | CTAG | 2 cut(s) | 87, 161 |
| MaeII | ACGT | 1 cut(s) | 252 |
| MaeIII | GTNAC | 2 cut(s) | 32, 437 |
| MboII | GAAGA | 2 cut(s) | 29, 473 |
| MhlI | GDGCHC | 1 cut(s) | 298 |
| MlsI | TGGCCA | 1 cut(s) | 167 |
| MluCI | AATT | 3 cut(s) | 104, 447, 493 |
| MluNI | TGGCCA | 1 cut(s) | 167 |
| MmeI | TCCRAC | 1 cut(s) | 379 |
| MnlI | CCTC | 4 cut(s) | 110, 211, 473, 480 |
| Mox20I | TGGCCA | 1 cut(s) | 167 |
| MroXI | GAANNNNTTC | 1 cut(s) | 21 |
| MscI | TGGCCA | 1 cut(s) | 167 |
| MseI | TTAA | 2 cut(s) | 249, 368 |
| Msp20I | TGGCCA | 1 cut(s) | 167 |
| MspCI | CTTAAG | 1 cut(s) | 367 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 156 |
| NheI | GCTAGC | 1 cut(s) | 86 |
| NlaIII | CATG | 5 cut(s) | 49, 248, 316, 445, 543 |
| NlaIV | GGNNCC | 1 cut(s) | 127 |
| NmuCI | GTSAC | 1 cut(s) | 437 |
| NspI | RCATGY | 1 cut(s) | 445 |
| PdmI | GAANNNNTTC | 1 cut(s) | 21 |
| PfeI | GAWTC | 3 cut(s) | 93, 238, 414 |
| PspN4I | GGNNCC | 1 cut(s) | 127 |
| PstI | CTGCAG | 1 cut(s) | 30 |
| RsaI | GTAC | 3 cut(s) | 59, 134, 338 |
| RsaNI | GTAC | 3 cut(s) | 58, 133, 337 |
| SaqAI | TTAA | 2 cut(s) | 249, 368 |
| SduI | GDGCHC | 1 cut(s) | 298 |
| SetI | ASST | 7 cut(s) | 59, 222, 255, 358, 374, 465, 510 |
| SfcI | CTRYAG | 1 cut(s) | 26 |
| SmlI | CTYRAG | 2 cut(s) | 367, 464 |
| SmoI | CTYRAG | 2 cut(s) | 367, 464 |
| Sse9I | AATT | 3 cut(s) | 104, 447, 493 |
| SsiI | CCGC | 2 cut(s) | 90, 148 |
| SspI | AATATT | 1 cut(s) | 384 |
| SspMI | CTAG | 2 cut(s) | 87, 161 |
| StyI | CCWWGG | 1 cut(s) | 504 |
| TaaI | ACNGT | 3 cut(s) | 130, 284, 293 |
| TaiI | ACGT | 1 cut(s) | 255 |
| TasI | AATT | 3 cut(s) | 104, 447, 493 |
| TatI | WGTACW | 2 cut(s) | 132, 336 |
| TfiI | GAWTC | 3 cut(s) | 93, 238, 414 |
| Tru1I | TTAA | 2 cut(s) | 249, 368 |
| Tru9I | TTAA | 2 cut(s) | 249, 368 |
| TscAI | CASTG | 1 cut(s) | 157 |
| TseFI | GTSAC | 1 cut(s) | 437 |
| Tsp45I | GTSAC | 1 cut(s) | 437 |
| TspDTI | ATGAA | 1 cut(s) | 301 |
| TspRI | CASTG | 1 cut(s) | 157 |
| Vha464I | CTTAAG | 1 cut(s) | 367 |
| VneI | GTGCAC | 1 cut(s) | 294 |
| XapI | RAATTY | 2 cut(s) | 447, 493 |
| XceI | RCATGY | 1 cut(s) | 445 |
| XmnI | GAANNNNTTC | 1 cut(s) | 21 |
| XspI | CTAG | 2 cut(s) | 87, 161 |