Prupe.6G272500_v2.0.a1

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001179
Physical Location & Seq
Reverse (-)
25784012 .. 25785647
1636 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G272500.1

Sequence Viewer

Length: 1359 bp
ATGTGGACTGATCTACTAGCTGCATATGCAATGTGGATGACCATGGCATACTTAACTGACGCATGGAAGCTCAACCTCAAGCACGCCGCTGCAATTGTCAATTTGTTCTGGGGCATCGTGGCCATAATGCCAGTGGGCTTGCAATTTGTTGTGGATACTTTCATGGGTTACTATTGGATGGTCTTGCTCTCCAGTTTTTCCTACATCGCTGGCTTAGGTTTTCTGTCAATGTTAACACCACCAGACCTTGCTGCTGCAACAGCCACTTGCAGCGCATATGATCCCGAGTGCATCGGCCAAGGGCAAAAAATCCTCTTTTTCACAGCTTTAGCTCTAATAGCTGTGGGATTTTCTGGTCACTTAGTCTCCGTACCACAATTCATGGCTGACCAGGACTCACACTCAAACCATAGAGACAGCATGCTCTGTCACTTGTTCATAAGCTTTACAGTGATCCACGTCCCCATAGCTGGAGCTTTTGCTATTTATTATATAAAGCCTTGGTCAATGAGGTATGGCATCCCTGCCATATGTACCTTGGTAGCAACACTTATTTTCTTGACTGGTTCGTGCTCATATCGCACTTATAGACCATACGGCAGCCCTCTCACTGTTCTGTTTAGGGTCTTTGTGGCCTCTGCCTCCAAAATTTTCCAGAAACACCCCAGAGACTCCTCTCATCTCTACGAGAGACGTGATGATTATTACTTGATACCTCACACTCGTCGCCTCAGGTGCCTAGACAAGGCTGCAATCATATTAGCCACCCAACCTCTACAGCAACAAGAAAATAATAGTTGGAGACTTTGTAGAGTCACAGAAGTGGAAGAAACCAAAAGCGTCCTATGCATGATTCCCATATGCATGACACTTATCCTAATTGGTGTTGTATCTTCAATTGGAACTATTCCTCTTCCAATCTTAGTATGGTTCTATACTCTTGCCAAACAACTATTTGGAAAACTTAACTTCCAAATTGCAGAACTAACAAGGTATGGTCCCCGAATTGGAATTGCCGTGTCAATGATTTTTGGAATTCTATGCTCAATCACAGCTGCAAAAGTGGAGACACGAAGGCTGGGCGTGGTTAAGAGCCACGGTCTAATCGACAAGCCTGAGGAAACGGTTCCCATGAGCATGTTTTGGTTGCTTCCACAGTTTCTTCTCCTCGGAGGCCTTGAAGGGATTGCAGAAAGTAGCATTGATTTGTTCTTAGTTAAGCAAGTCCCTCCATCCATTGATCAGTGCATGGTTCGGTTTTGTGTTGCTTTTTTGGGAGTGGGGAGTATAGGCGGTGTCTTGTCTGTTTATGTAGTGGGTGAGATAAGTGGACAGGGAGGGAAACCCAGTTGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

453

Amino Acids

50.23

Weight (kDa)

8.52

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 735
AciI CCGC 2 cut(s) 87, 1293
AclWI GGATC 2 cut(s) 275, 448
AcoI YGGCCR 2 cut(s) 120, 295
AcsI RAATTY 2 cut(s) 648, 1035
AfaI GTAC 2 cut(s) 372, 535
AfiI CCNNNNNNNGG 3 cut(s) 470, 745, 1007
AgsI TTSAA 2 cut(s) 897, 1181
AjiI CACGTC 2 cut(s) 460, 695
AjnI CCWGG 1 cut(s) 390
AleI CACNNNNGTG 1 cut(s) 821
AluBI AGCT 9 cut(s) 20, 70, 326, 332, 341, 444, 470, 476, 1055
AluI AGCT 9 cut(s) 20, 70, 326, 332, 341, 444, 470, 476, 1055
Alw21I GWGCWC 1 cut(s) 575
Alw26I GTCTC 6 cut(s) 370, 408, 663, 685, 796, 1061
AlwI GGATC 2 cut(s) 275, 448
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 4 cut(s) 120, 295, 633, 1174
ApeKI GCWGC 8 cut(s) 20, 89, 251, 254, 270, 600, 749, 1055
ApoI RAATTY 2 cut(s) 648, 1035
Asp700I GAANNNNTTC 1 cut(s) 1125
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 998
AsuHPI GGTGA 1 cut(s) 1331
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 998
AxyI CCTNAGG 2 cut(s) 731, 1116
BalI TGGCCA 1 cut(s) 122
BanI GGYRCC 1 cut(s) 735
Bbv12I GWGCWC 1 cut(s) 575
BbvI GCAGC 8 cut(s) 7, 76, 238, 241, 282, 612, 736, 1042
BccI CCATC 2 cut(s) 172, 1240
BceAI ACGGC 2 cut(s) 613, 1001
BciT130I CCWGG 1 cut(s) 392
BciVI GTATCC 1 cut(s) 148
BclI TGATCA 1 cut(s) 1240
BcoDI GTCTC 6 cut(s) 370, 408, 663, 685, 796, 1061
BfaI CTAG 2 cut(s) 17, 740
BfmI CTRYAG 1 cut(s) 776
BfuI GTATCC 1 cut(s) 148
BisI GCNGC 9 cut(s) 21, 87, 90, 252, 255, 271, 601, 750, 1056
BlsI GCNGC 9 cut(s) 22, 88, 91, 253, 256, 272, 602, 751, 1057
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 1 cut(s) 998
BmeT110I CYCGRG 1 cut(s) 284
BmgBI CACGTC 2 cut(s) 460, 695
BmgT120I GGNCC 1 cut(s) 998
BmiI GGNNCC 3 cut(s) 737, 1000, 1128
BmrFI CCNGG 1 cut(s) 392
BmrI ACTGGG 1 cut(s) 1341
BmsI GCATC 3 cut(s) 123, 300, 528
BmuI ACTGGG 1 cut(s) 1341
BpmI CTGGAG 2 cut(s) 175, 492
Bpu10I CCTNAGC 1 cut(s) 214
BpuEI CTTGAG 1 cut(s) 62
BsaJI CCNNGG 6 cut(s) 42, 298, 500, 537, 1096, 1168
BsaXI ACNNNNNCTCC 4 cut(s) 350, 380, 793, 823
Bsc4I CCNNNNNNNGG 3 cut(s) 470, 745, 1007
Bse1I ACTGG 4 cut(s) 131, 192, 568, 1347
Bse21I CCTNAGG 2 cut(s) 731, 1116
Bse3DI GCAATG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 392
BseDI CCNNGG 6 cut(s) 42, 298, 500, 537, 1096, 1168
BseGI GGATG 4 cut(s) 42, 183, 519, 1232
BseLI CCNNNNNNNGG 3 cut(s) 470, 745, 1007
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 2 cut(s) 745, 1107
BseNI ACTGG 4 cut(s) 131, 192, 568, 1347
BseRI GAGGAG 2 cut(s) 664, 1157
BseXI GCAGC 8 cut(s) 7, 76, 238, 241, 282, 612, 736, 1042
BseYI CCCAGC 1 cut(s) 1078
BshFI GGCC 4 cut(s) 122, 297, 635, 1176
BshNI GGYRCC 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 575
BsiHKCI CYCGRG 1 cut(s) 284
BslFI GGGAC 3 cut(s) 446, 984, 1211
BslI CCNNNNNNNGG 3 cut(s) 470, 745, 1007
BsmAI GTCTC 6 cut(s) 370, 408, 663, 685, 796, 1061
BsmBI CGTCTC 1 cut(s) 685
BsmFI GGGAC 3 cut(s) 446, 984, 1211
BsnI GGCC 4 cut(s) 122, 297, 635, 1176
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 1 cut(s) 575
Bsp143I GATC 4 cut(s) 10, 280, 453, 1240
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 2 cut(s) 87, 1293
BspANI GGCC 4 cut(s) 122, 297, 635, 1176
BspCNI CTCAG 2 cut(s) 744, 1108
BspLI GGNNCC 3 cut(s) 737, 1000, 1128
BspPI GGATC 2 cut(s) 275, 448
BspT107I GGYRCC 1 cut(s) 735
BsrDI GCAATG 1 cut(s) 36
BsrI ACTGG 4 cut(s) 131, 192, 568, 1347
BssECI CCNNGG 6 cut(s) 42, 298, 500, 537, 1096, 1168
BssMI GATC 4 cut(s) 10, 280, 453, 1240
BssT1I CCWWGG 4 cut(s) 42, 298, 500, 537
Bst2UI CCWGG 1 cut(s) 392
Bst4CI ACNGT 5 cut(s) 451, 613, 1100, 1126, 1158
Bst6I CTCTTC 1 cut(s) 918
BstC8I GCNNGC 4 cut(s) 84, 140, 211, 422
BstDEI CTNAG 6 cut(s) 214, 361, 731, 922, 1116, 1213
BstDSI CCRYGG 2 cut(s) 42, 1096
BstENI CCTNNNNNAGG 1 cut(s) 743
BstF5I GGATG 4 cut(s) 42, 183, 519, 1232
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 4 cut(s) 13, 283, 456, 1243
BstMAI GTCTC 6 cut(s) 370, 408, 663, 685, 796, 1061
BstMBI GATC 4 cut(s) 10, 280, 453, 1240
BstMWI GCNNNNNNNGC 6 cut(s) 26, 260, 338, 579, 735, 846
BstNI CCWGG 1 cut(s) 392
BstNSI RCATGY 2 cut(s) 424, 1141
BstSCI CCNGG 1 cut(s) 390
BstSFI CTRYAG 1 cut(s) 776
BstV1I GCAGC 8 cut(s) 7, 76, 238, 241, 282, 612, 736, 1042
Bsu36I CCTNAGG 2 cut(s) 731, 1116
BsuI GTATCC 1 cut(s) 148
BsuRI GGCC 4 cut(s) 122, 297, 635, 1176
BtgI CCRYGG 2 cut(s) 42, 1096
BtgZI GCGATG 1 cut(s) 190
BtrI CACGTC 2 cut(s) 460, 695
BtsCI GGATG 4 cut(s) 42, 183, 519, 1232
BtsIMutI CAGTG 4 cut(s) 138, 456, 609, 1250
Cac8I GCNNGC 4 cut(s) 84, 140, 211, 422
CfoI GCGC 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 998
CseI GACGC 2 cut(s) 68, 829
Csp6I GTAC 2 cut(s) 371, 534
CviQI GTAC 2 cut(s) 371, 534
DdeI CTNAG 6 cut(s) 214, 361, 731, 922, 1116, 1213
DpnI GATC 4 cut(s) 12, 282, 455, 1242
DpnII GATC 4 cut(s) 10, 280, 453, 1240
EaeI YGGCCR 2 cut(s) 120, 295
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
Eco130I CCWWGG 4 cut(s) 42, 298, 500, 537
Eco147I AGGCCT 1 cut(s) 1176
Eco47I GGWCC 1 cut(s) 998
Eco81I CCTNAGG 2 cut(s) 731, 1116
Eco88I CYCGRG 1 cut(s) 284
EcoNI CCTNNNNNAGG 1 cut(s) 743
EcoRI GAATTC 1 cut(s) 1035
EcoRII CCWGG 1 cut(s) 390
EcoT14I CCWWGG 4 cut(s) 42, 298, 500, 537
EcoT22I ATGCAT 2 cut(s) 851, 866
ErhI CCWWGG 4 cut(s) 42, 298, 500, 537
Esp3I CGTCTC 1 cut(s) 685
FaqI GGGAC 3 cut(s) 446, 984, 1211
FauNDI CATATG 4 cut(s) 25, 277, 530, 860
FbaI TGATCA 1 cut(s) 1240
Fnu4HI GCNGC 9 cut(s) 21, 87, 90, 252, 255, 271, 601, 750, 1056
FokI GGATG 4 cut(s) 49, 190, 506, 1219
Fsp4HI GCNGC 9 cut(s) 21, 87, 90, 252, 255, 271, 601, 750, 1056
FspBI CTAG 2 cut(s) 17, 740
GlaI GCGC 1 cut(s) 274
GluI GCNGC 9 cut(s) 21, 87, 90, 252, 255, 271, 601, 750, 1056
GsaI CCCAGC 1 cut(s) 1082
GsuI CTGGAG 2 cut(s) 175, 492
HaeIII GGCC 4 cut(s) 122, 297, 635, 1176
HgaI GACGC 2 cut(s) 68, 829
HhaI GCGC 1 cut(s) 275
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 442
HinfI GANTC 4 cut(s) 395, 671, 813, 853
HpaI GTTAAC 1 cut(s) 234
HphI GGTGA 1 cut(s) 1331
Hpy166II GTNNAC 3 cut(s) 6, 234, 1331
Hpy188I TCNGA 1 cut(s) 1172
Hpy188III TCNNGA 3 cut(s) 284, 559, 655
Hpy8I GTNNAC 3 cut(s) 6, 234, 1331
Hpy99I CGWCG 1 cut(s) 729
HpyAV CCTTC 2 cut(s) 1068, 1175
HpyCH4III ACNGT 5 cut(s) 451, 613, 1100, 1126, 1158
HpyCH4IV ACGT 2 cut(s) 459, 694
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 260, 338, 579, 735, 846
HpyF3I CTNAG 6 cut(s) 214, 361, 731, 922, 1116, 1213
HpySE526I ACGT 2 cut(s) 459, 694
HspAI GCGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 1240
KspAI GTTAAC 1 cut(s) 234
Kzo9I GATC 4 cut(s) 10, 280, 453, 1240
LmnI GCTCC 1 cut(s) 473
Lsp1109I GCAGC 8 cut(s) 7, 76, 238, 241, 282, 612, 736, 1042
LweI GCATC 3 cut(s) 123, 300, 528
MaeI CTAG 2 cut(s) 17, 740
MaeII ACGT 2 cut(s) 459, 694
MaeIII GTNAC 4 cut(s) 167, 356, 428, 814
MalI GATC 4 cut(s) 12, 282, 455, 1242
MboI GATC 4 cut(s) 10, 280, 453, 1240
MboII GAAGA 4 cut(s) 839, 885, 905, 1154
MfeI CAATTG 2 cut(s) 93, 897
MhlI GDGCHC 1 cut(s) 575
MlsI TGGCCA 1 cut(s) 122
MluNI TGGCCA 1 cut(s) 122
MlyI GAGTC 3 cut(s) 389, 665, 822
MmeI TCCRAC 1 cut(s) 779
Mox20I TGGCCA 1 cut(s) 122
Mph1103I ATGCAT 2 cut(s) 851, 866
MroXI GAANNNNTTC 1 cut(s) 1125
MscI TGGCCA 1 cut(s) 122
MseI TTAA 5 cut(s) 53, 233, 966, 1089, 1218
MslI CAYNNNNRTG 3 cut(s) 821, 863, 1136
Msp20I TGGCCA 1 cut(s) 122
MspA1I CMGCKG 2 cut(s) 89, 1055
MspR9I CCNGG 1 cut(s) 392
MunI CAATTG 2 cut(s) 93, 897
MvaI CCWGG 1 cut(s) 392
MwoI GCNNNNNNNGC 6 cut(s) 26, 260, 338, 579, 735, 846
NcoI CCATGG 1 cut(s) 42
NdeI CATATG 4 cut(s) 25, 277, 530, 860
NdeII GATC 4 cut(s) 10, 280, 453, 1240
NlaIV GGNNCC 3 cut(s) 737, 1000, 1128
NmuCI GTSAC 3 cut(s) 356, 428, 814
NsiI ATGCAT 2 cut(s) 851, 866
NspI RCATGY 2 cut(s) 424, 1141
OliI CACNNNNGTG 1 cut(s) 821
PaeI GCATGC 1 cut(s) 424
PceI AGGCCT 1 cut(s) 1176
PcsI WCGNNNNNNNCGW 1 cut(s) 1104
PdmI GAANNNNTTC 1 cut(s) 1125
PfeI GAWTC 1 cut(s) 853
PkrI GCNGC 9 cut(s) 22, 88, 91, 253, 256, 272, 602, 751, 1057
PleI GAGTC 3 cut(s) 389, 665, 821
PpsI GAGTC 3 cut(s) 389, 665, 821
Psp6I CCWGG 1 cut(s) 390
PspFI CCCAGC 1 cut(s) 1078
PspGI CCWGG 1 cut(s) 390
PspN4I GGNNCC 3 cut(s) 737, 1000, 1128
PspPI GGNCC 1 cut(s) 998
PvuII CAGCTG 1 cut(s) 1055
RsaI GTAC 2 cut(s) 372, 535
RsaNI GTAC 2 cut(s) 371, 534
RseI CAYNNNNRTG 3 cut(s) 821, 863, 1136
SaqAI TTAA 5 cut(s) 53, 233, 966, 1089, 1218
SatI GCNGC 9 cut(s) 21, 87, 90, 252, 255, 271, 601, 750, 1056
Sau3AI GATC 4 cut(s) 10, 280, 453, 1240
Sau96I GGNCC 1 cut(s) 998
SchI GAGTC 3 cut(s) 389, 665, 822
ScrFI CCNGG 1 cut(s) 392
SduI GDGCHC 1 cut(s) 575
SfaNI GCATC 3 cut(s) 123, 300, 528
SfcI CTRYAG 1 cut(s) 776
SinI GGWCC 1 cut(s) 998
SmiMI CAYNNNNRTG 3 cut(s) 821, 863, 1136
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SphI GCATGC 1 cut(s) 424
SseBI AGGCCT 1 cut(s) 1176
SsiI CCGC 2 cut(s) 87, 1293
SspMI CTAG 2 cut(s) 17, 740
StuI AGGCCT 1 cut(s) 1176
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 4 cut(s) 42, 298, 500, 537
TaaI ACNGT 5 cut(s) 451, 613, 1100, 1126, 1158
TaiI ACGT 2 cut(s) 462, 697
TaqI TCGA 1 cut(s) 1107
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 1 cut(s) 853
Tru1I TTAA 5 cut(s) 53, 233, 966, 1089, 1218
Tru9I TTAA 5 cut(s) 53, 233, 966, 1089, 1218
TscAI CASTG 4 cut(s) 138, 456, 616, 1250
TseFI GTSAC 3 cut(s) 356, 428, 814
TseI GCWGC 8 cut(s) 20, 89, 251, 254, 270, 600, 749, 1055
Tsp45I GTSAC 3 cut(s) 356, 428, 814
TspDTI ATGAA 3 cut(s) 151, 370, 427
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 4 cut(s) 138, 456, 616, 1250
VpaK11BI GGWCC 1 cut(s) 998
XagI CCTNNNNNAGG 1 cut(s) 743
XapI RAATTY 2 cut(s) 648, 1035
XceI RCATGY 2 cut(s) 424, 1141
XcmI CCANNNNNNNNNTGG 3 cut(s) 130, 535, 924
XmnI GAANNNNTTC 1 cut(s) 1125
XspI CTAG 2 cut(s) 17, 740
Zsp2I ATGCAT 2 cut(s) 851, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.