MD04G1148300.v1.1

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001179
Physical Location & Seq
Reverse (-)
23666705 .. 23668537
1833 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1148300.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGTGGGCGGATATACTTGCTGCATATGCAATGTGGATGACCATGGCATACTTAACAGATGCATGGAAGCTCAGTCCCACCCACGCGGCTGGAATTCTCAATATTTTCTGGGGCATGGTGGCCATAATGCCAGTGGGCTTGCAGTTTCTTGTGGATGCTTTCATGGGTAACTTCTGGATGGTCTTAGTTTCCAGTTTTTCCTACTGTGTGGGCTTAGGTTTTTTGTCAATGTCAACGCCACCAGTCCTTACCGGGGCAACAGGCACTTGCAGCGCATATGAGCCCGAGTGCATTGGCCAAGCACAAAAGGCCCTCTTTTTCACAGCGTTAGCCCTAATAGCTGTGGGAGTTTGCGGTCACTTGACCTCCTTAGGGACGTTCATTGCTGAGCAGTTCATGCGCCCAGACGAAGACGCAGACTCAGACCTTGAGGAGGGCAGGCTCTTGCAATTTTTCCTCAGCTGCTTAGCCTCCGTTATGGCAATCCTCGTCCCTATAGCTGGAGTTTTTGCAGCTTATTTTATAAAGCCTTGGTCAATTAGGTATGGCATCCCAGCCATATGTACCTTGGTAGCTCTACTTATTTTCTTAACTGGTTCGCGCTCGTATCACACTCCTAGAGGACAAGGCAGCCCTCTCACAACTCTGATTAGGGTCTTTGTGGCCTCAGCCTCTAAAATTTTCCAGAAATGCCCGAGAGATGCCTCTCAGCTCTATGAGAGACATGATGTTGATTATTGCATACCTCACACTAACCACCTCAGGTGCCTAGACAAGGCTGCCATTTTATCAGCCACCCAACCTCTAGAGCAACAACAAAATAATAGGTGGAGACTTTGCAGAGTCACAGAAGTGGAAGAAACCAAAAGCATCCTATGCATGATTCCTCTATGCACAACCTTTATCCTATTGGGTGTTGTAACTTCAATCGGAAATACCTACTTCATCGAGCAAGCAATCCACATGAACCGCAAAGTTGGGCGAATAAAGGTTCCTCTTCCATTGCTTCTATGGTTCTATGACCAAGCCAAACAATTTTGTGGAAAACTCACACAACTTTTAGGACTAACAAGGTATGGTCCCCCAATTGGAATTGGTGTATCAATGATTTTTGCAATTCTATGTTGCATCACGGCAGCGAAAGTGGAGACACGAAGGCTAGGCGTGGTTGCAAGCCATGGTCTAATTGACAAGCCGGAGGAAACAATTTCCATGACCATATTTTGGTTGCTTCCACAGTTTCTTCTCCTAGGAGGTGCTGATGGGATTGCAGAAAATAGCATTGCTTTGTTCTTCATTAATCAGGTTCCTCCGTCCATGGCGCAGTACATGGTTATGTTTGCCATTGCTGTTTTTGGAGCTGGGACAATGGGCAGTACTTTGTCGGTTCATTTAGTTGGTAAGATAAGTGGAAGGGGAGGGAGATCCAGTTGGTTTCAAGACACTTTGAACAAAAGTCGTTTGGATAATTACTATTGGACCTTGGCTGCACTGGCTGCAGCTAATCTTGTTTTCTATATTTTGATGAGCTTTTGGTATGCTTATAACGATTCGAAATCGGAGGAGGAAGAAGATTCTGGTGCAACTGGTCAGGAACTCGAATTTCAAGTGGAGTGCTCCTGTTGCTGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

545

Amino Acids

60.03

Weight (kDa)

6.02

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 58 - 478 1.4e-34 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 524, 1545
AccB1I GGYRCC 1 cut(s) 765
AccB7I CCANNNNNTGG 1 cut(s) 1224
AccII CGCG 2 cut(s) 86, 601
AciI CCGC 4 cut(s) 8, 86, 354, 970
AclWI GGATC 1 cut(s) 1419
AcoI YGGCCR 2 cut(s) 120, 295
AcsI RAATTY 3 cut(s) 93, 678, 1601
AfaI GTAC 3 cut(s) 565, 1328, 1378
AfiI CCNNNNNNNGG 7 cut(s) 253, 372, 433, 500, 775, 1088, 1224
AgsI TTSAA 4 cut(s) 927, 1439, 1450, 1607
AleI CACNNNNGTG 1 cut(s) 851
Alw21I GWGCWC 1 cut(s) 1619
Alw26I GTCTC 3 cut(s) 715, 826, 1142
AlwI GGATC 1 cut(s) 1419
Ama87I CYCGRG 2 cut(s) 284, 694
AoxI GGCC 4 cut(s) 120, 295, 309, 663
ApoI RAATTY 3 cut(s) 93, 678, 1601
AseI ATTAAT 1 cut(s) 1299
AspA2I CCTAGG 1 cut(s) 1249
AspLEI GCGC 4 cut(s) 275, 402, 603, 1324
AspS9I GGNCC 3 cut(s) 310, 1079, 1479
AsuC2I CCSGG 1 cut(s) 253
AsuII TTCGAA 1 cut(s) 1553
AvaI CYCGRG 2 cut(s) 284, 694
AvaII GGWCC 2 cut(s) 1079, 1479
AvrII CCTAGG 1 cut(s) 1249
AxyI CCTNAGG 2 cut(s) 370, 761
BalI TGGCCA 2 cut(s) 122, 297
BanI GGYRCC 1 cut(s) 765
BanII GRGCYC 1 cut(s) 285
BbsI GAAGAC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 1619
BbvCI CCTCAGC 2 cut(s) 458, 667
BccI CCATC 2 cut(s) 172, 1256
BceAI ACGGC 1 cut(s) 1149
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 3 cut(s) 715, 826, 1142
BfaI CTAG 5 cut(s) 618, 770, 806, 1160, 1250
BfmI CTRYAG 2 cut(s) 495, 1497
BlnI CCTAGG 1 cut(s) 1249
BlpI GCTNAGC 2 cut(s) 387, 466
BmcAI AGTACT 1 cut(s) 1378
Bme1390I CCNGG 1 cut(s) 253
Bme18I GGWCC 2 cut(s) 1079, 1479
BmeT110I CYCGRG 2 cut(s) 284, 694
BmgT120I GGNCC 3 cut(s) 310, 1079, 1479
BmiI GGNNCC 4 cut(s) 767, 993, 1081, 1308
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 6 cut(s) 49, 145, 558, 691, 879, 1137
BpiI GAAGAC 1 cut(s) 417
BpmI CTGGAG 1 cut(s) 522
Bpu10I CCTNAGC 3 cut(s) 214, 458, 667
Bpu1102I GCTNAGC 2 cut(s) 387, 466
Bpu14I TTCGAA 1 cut(s) 1553
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 253
BsaJI CCNNGG 8 cut(s) 42, 252, 530, 567, 1177, 1249, 1317, 1482
BsaXI ACNNNNNCTCC 4 cut(s) 350, 380, 1414, 1444
Bsc4I CCNNNNNNNGG 7 cut(s) 253, 372, 433, 500, 775, 1088, 1224
Bse1I ACTGG 7 cut(s) 131, 192, 242, 598, 1428, 1497, 1591
Bse21I CCTNAGG 2 cut(s) 370, 761
Bse3DI GCAATG 5 cut(s) 36, 381, 1001, 1281, 1344
BseDI CCNNGG 8 cut(s) 42, 252, 530, 567, 1177, 1249, 1317, 1482
BseGI GGATG 5 cut(s) 42, 160, 183, 549, 870
BseLI CCNNNNNNNGG 7 cut(s) 253, 372, 433, 500, 775, 1088, 1224
BseMI GCAATG 5 cut(s) 36, 381, 1001, 1281, 1344
BseMII CTCAG 7 cut(s) 85, 378, 435, 472, 681, 722, 775
BseNI ACTGG 7 cut(s) 131, 192, 242, 598, 1428, 1497, 1591
BseRI GAGGAG 2 cut(s) 446, 1577
BseYI CCCAGC 2 cut(s) 553, 1361
BsgI GTGCAG 1 cut(s) 1473
Bsh1236I CGCG 2 cut(s) 86, 601
BshFI GGCC 4 cut(s) 122, 297, 311, 665
BshNI GGYRCC 1 cut(s) 765
BsiHKAI GWGCWC 1 cut(s) 1619
BsiHKCI CYCGRG 2 cut(s) 284, 694
BsiSI CCGG 2 cut(s) 252, 1196
BslFI GGGAC 5 cut(s) 60, 388, 476, 1065, 1378
BslI CCNNNNNNNGG 7 cut(s) 253, 372, 433, 500, 775, 1088, 1224
BsmAI GTCTC 3 cut(s) 715, 826, 1142
BsmFI GGGAC 5 cut(s) 60, 388, 476, 1065, 1378
BsnI GGCC 4 cut(s) 122, 297, 311, 665
BsoBI CYCGRG 2 cut(s) 284, 694
Bsp119I TTCGAA 1 cut(s) 1553
Bsp1286I GDGCHC 2 cut(s) 285, 1619
Bsp143I GATC 1 cut(s) 1424
Bsp1720I GCTNAGC 2 cut(s) 387, 466
Bsp19I CCATGG 3 cut(s) 42, 1177, 1317
BspACI CCGC 4 cut(s) 8, 86, 354, 970
BspANI GGCC 4 cut(s) 122, 297, 311, 665
BspCNI CTCAG 7 cut(s) 84, 379, 434, 471, 680, 721, 774
BspFNI CGCG 2 cut(s) 86, 601
BspLI GGNNCC 4 cut(s) 767, 993, 1081, 1308
BspMAI CTGCAG 1 cut(s) 1501
BspPI GGATC 1 cut(s) 1419
BspT104I TTCGAA 1 cut(s) 1553
BspT107I GGYRCC 1 cut(s) 765
BsrDI GCAATG 5 cut(s) 36, 381, 1001, 1281, 1344
BsrI ACTGG 7 cut(s) 131, 192, 242, 598, 1428, 1497, 1591
BssECI CCNNGG 8 cut(s) 42, 252, 530, 567, 1177, 1249, 1317, 1482
BssMI GATC 1 cut(s) 1424
BssT1I CCWWGG 7 cut(s) 42, 530, 567, 1177, 1249, 1317, 1482
Bst4CI ACNGT 2 cut(s) 206, 1239
Bst6I CTCTTC 1 cut(s) 1002
BstAPI GCANNNNNTGC 3 cut(s) 397, 876, 1496
BstBI TTCGAA 1 cut(s) 1553
BstC8I GCNNGC 4 cut(s) 140, 440, 954, 1174
BstDSI CCRYGG 3 cut(s) 42, 1177, 1317
BstENI CCTNNNNNAGG 2 cut(s) 431, 773
BstF5I GGATG 5 cut(s) 42, 160, 183, 549, 870
BstFNI CGCG 2 cut(s) 86, 601
BstHHI GCGC 4 cut(s) 275, 402, 603, 1324
BstKTI GATC 1 cut(s) 1427
BstMAI GTCTC 3 cut(s) 715, 826, 1142
BstMBI GATC 1 cut(s) 1424
BstMWI GCNNNNNNNGC 9 cut(s) 26, 270, 308, 338, 397, 876, 1493, 1496, 1623
BstSCI CCNGG 1 cut(s) 251
BstSFI CTRYAG 2 cut(s) 495, 1497
BstUI CGCG 2 cut(s) 86, 601
BstV2I GAAGAC 1 cut(s) 417
BstX2I RGATCY 1 cut(s) 1424
BstXI CCANNNNNNTGG 1 cut(s) 89
BstYI RGATCY 1 cut(s) 1424
Bsu36I CCTNAGG 2 cut(s) 370, 761
BsuRI GGCC 4 cut(s) 122, 297, 311, 665
BtgI CCRYGG 3 cut(s) 42, 1177, 1317
BtsCI GGATG 5 cut(s) 42, 160, 183, 549, 870
BtsIMutI CAGTG 2 cut(s) 138, 1490
Cac8I GCNNGC 4 cut(s) 140, 440, 954, 1174
CfoI GCGC 4 cut(s) 275, 402, 603, 1324
Cfr13I GGNCC 3 cut(s) 310, 1079, 1479
CseI GACGC 1 cut(s) 422
Csp6I GTAC 3 cut(s) 564, 1327, 1377
CviQI GTAC 3 cut(s) 564, 1327, 1377
DpnI GATC 1 cut(s) 1426
DpnII GATC 1 cut(s) 1424
EaeI YGGCCR 2 cut(s) 120, 295
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 7 cut(s) 42, 530, 567, 1177, 1249, 1317, 1482
Eco24I GRGCYC 1 cut(s) 285
Eco47I GGWCC 2 cut(s) 1079, 1479
Eco81I CCTNAGG 2 cut(s) 370, 761
Eco88I CYCGRG 2 cut(s) 284, 694
EcoNI CCTNNNNNAGG 2 cut(s) 431, 773
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 93
EcoT14I CCWWGG 7 cut(s) 42, 530, 567, 1177, 1249, 1317, 1482
EcoT22I ATGCAT 2 cut(s) 64, 881
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 7 cut(s) 42, 530, 567, 1177, 1249, 1317, 1482
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 5 cut(s) 60, 388, 476, 1065, 1378
FauNDI CATATG 3 cut(s) 25, 277, 560
FokI GGATG 5 cut(s) 49, 167, 190, 536, 857
FriOI GRGCYC 1 cut(s) 285
FspBI CTAG 5 cut(s) 618, 770, 806, 1160, 1250
GlaI GCGC 4 cut(s) 274, 401, 602, 1323
GsaI CCCAGC 2 cut(s) 557, 1365
GsuI CTGGAG 1 cut(s) 522
HaeIII GGCC 4 cut(s) 122, 297, 311, 665
HapII CCGG 2 cut(s) 252, 1196
HgaI GACGC 1 cut(s) 422
HhaI GCGC 4 cut(s) 275, 402, 603, 1324
Hin6I GCGC 4 cut(s) 273, 400, 601, 1322
HinP1I GCGC 4 cut(s) 273, 400, 601, 1322
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HinfI GANTC 5 cut(s) 419, 843, 883, 1550, 1574
HpaII CCGG 2 cut(s) 252, 1196
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 4 cut(s) 424, 648, 932, 1561
Hpy188III TCNNGA 5 cut(s) 175, 685, 806, 1439, 1592
Hpy8I GTNNAC 1 cut(s) 234
HpyAV CCTTC 2 cut(s) 1149, 1407
HpyCH4III ACNGT 2 cut(s) 206, 1239
HpyCH4IV ACGT 1 cut(s) 377
HpyF10VI GCNNNNNNNGC 9 cut(s) 26, 270, 308, 338, 397, 876, 1493, 1496, 1623
HpySE526I ACGT 1 cut(s) 377
HspAI GCGC 4 cut(s) 273, 400, 601, 1322
Kzo9I GATC 1 cut(s) 1424
LmnI GCTCC 2 cut(s) 1358, 1622
LweI GCATC 6 cut(s) 49, 145, 558, 691, 879, 1137
MaeI CTAG 5 cut(s) 618, 770, 806, 1160, 1250
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 4 cut(s) 167, 356, 844, 919
MalI GATC 1 cut(s) 1426
MboI GATC 1 cut(s) 1424
MboII GAAGA 7 cut(s) 422, 869, 989, 1235, 1285, 1580, 1583
MfeI CAATTG 1 cut(s) 1086
MflI RGATCY 1 cut(s) 1424
MhlI GDGCHC 2 cut(s) 285, 1619
MlsI TGGCCA 2 cut(s) 122, 297
MluNI TGGCCA 2 cut(s) 122, 297
MlyI GAGTC 2 cut(s) 413, 852
Mox20I TGGCCA 2 cut(s) 122, 297
Mph1103I ATGCAT 2 cut(s) 64, 881
MscI TGGCCA 2 cut(s) 122, 297
MseI TTAA 3 cut(s) 53, 590, 1299
MslI CAYNNNNRTG 2 cut(s) 851, 1334
Msp20I TGGCCA 2 cut(s) 122, 297
MspA1I CMGCKG 1 cut(s) 462
MspI CCGG 2 cut(s) 252, 1196
MspR9I CCNGG 1 cut(s) 253
MunI CAATTG 1 cut(s) 1086
MvnI CGCG 2 cut(s) 86, 601
MwoI GCNNNNNNNGC 9 cut(s) 26, 270, 308, 338, 397, 876, 1493, 1496, 1623
NciI CCSGG 1 cut(s) 253
NcoI CCATGG 3 cut(s) 42, 1177, 1317
NdeI CATATG 3 cut(s) 25, 277, 560
NdeII GATC 1 cut(s) 1424
NlaIV GGNNCC 4 cut(s) 767, 993, 1081, 1308
NmuCI GTSAC 2 cut(s) 356, 844
NsiI ATGCAT 2 cut(s) 64, 881
NspV TTCGAA 1 cut(s) 1553
OliI CACNNNNGTG 1 cut(s) 851
PfeI GAWTC 3 cut(s) 883, 1550, 1574
PflMI CCANNNNNTGG 1 cut(s) 1224
PleI GAGTC 2 cut(s) 413, 851
PpsI GAGTC 2 cut(s) 413, 851
PshBI ATTAAT 1 cut(s) 1299
PsiI TTATAA 2 cut(s) 524, 1545
PspFI CCCAGC 2 cut(s) 553, 1361
PspN4I GGNNCC 4 cut(s) 767, 993, 1081, 1308
PspPI GGNCC 3 cut(s) 310, 1079, 1479
PstI CTGCAG 1 cut(s) 1501
PsuI RGATCY 1 cut(s) 1424
PvuII CAGCTG 1 cut(s) 462
RsaI GTAC 3 cut(s) 565, 1328, 1378
RsaNI GTAC 3 cut(s) 564, 1327, 1377
RseI CAYNNNNRTG 2 cut(s) 851, 1334
SaqAI TTAA 3 cut(s) 53, 590, 1299
Sau3AI GATC 1 cut(s) 1424
Sau96I GGNCC 3 cut(s) 310, 1079, 1479
ScaI AGTACT 1 cut(s) 1378
SchI GAGTC 2 cut(s) 413, 852
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 2 cut(s) 285, 1619
SfaNI GCATC 6 cut(s) 49, 145, 558, 691, 879, 1137
SfcI CTRYAG 2 cut(s) 495, 1497
SfuI TTCGAA 1 cut(s) 1553
SinI GGWCC 2 cut(s) 1079, 1479
SmiMI CAYNNNNRTG 2 cut(s) 851, 1334
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
SsiI CCGC 4 cut(s) 8, 86, 354, 970
SspI AATATT 1 cut(s) 103
SspMI CTAG 5 cut(s) 618, 770, 806, 1160, 1250
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 7 cut(s) 42, 530, 567, 1177, 1249, 1317, 1482
TaaI ACNGT 2 cut(s) 206, 1239
TaiI ACGT 1 cut(s) 380
TaqI TCGA 3 cut(s) 948, 1553, 1599
TatI WGTACW 2 cut(s) 1326, 1376
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 3 cut(s) 883, 1550, 1574
Tru1I TTAA 3 cut(s) 53, 590, 1299
Tru9I TTAA 3 cut(s) 53, 590, 1299
TscAI CASTG 2 cut(s) 138, 1497
TseFI GTSAC 2 cut(s) 356, 844
Tsp45I GTSAC 2 cut(s) 356, 844
TspDTI ATGAA 7 cut(s) 151, 370, 385, 934, 980, 1285, 1379
TspGWI ACGGA 2 cut(s) 463, 1302
TspRI CASTG 2 cut(s) 138, 1497
Van91I CCANNNNNTGG 1 cut(s) 1224
VpaK11BI GGWCC 2 cut(s) 1079, 1479
VspI ATTAAT 1 cut(s) 1299
XagI CCTNNNNNAGG 2 cut(s) 431, 773
XapI RAATTY 3 cut(s) 93, 678, 1601
XbaI TCTAGA 1 cut(s) 805
XcmI CCANNNNNNNNNTGG 3 cut(s) 130, 565, 1008
XmaJI CCTAGG 1 cut(s) 1249
XspI CTAG 5 cut(s) 618, 770, 806, 1160, 1250
ZrmI AGTACT 1 cut(s) 1378
Zsp2I ATGCAT 2 cut(s) 64, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.