Prupe.6G272600_v2.0.a1

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001179
Physical Location & Seq
Reverse (-)
25786765 .. 25789407
2643 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G272600.1

Sequence Viewer

Length: 1680 bp
ATGCATAACATTTTCTTTAATTCTTTTGCTTCTGCTCCTAAACTGTATGTAGTGCTGACATGGGCGGATGTACTCGCTGTGTACGCAATGTGGATGACCATGGTATACTTAACGGAGGTATGGAAGCTCAATTTCACACATGCTGCTGCAATTGTCAATTTGTACTGGGGCATTGTAGCCGTACTACCAGTGGGCTTGCAATTTCTTGTGGATGCTTTCATGGGTAACTATTGGATGGTCTTGGTCTCCAGTTTTGCCTACAGTGCTGGCTTGGGTTTTTTGTCAATGTCAACACCACCAGTTCTTGCTGGGGCAACAGGCACTTGCAGCGCATATGAGCCGGAGTGCATAGGCCAAGGACAAAAAATCCTCTTTTATACAGCAGTAGCTCTAATAGCTATGGGAATGTCTGGTCATTTAACCTCATTAGGGGCATTCATTGCTGAGCAGTTTATGGACTCACAATCAAACCTTGAAGACAGCATGCCCTGTCACTTTTTCCTGAGCTTCTTGATGGTGATCCTCGTCCCTATAGCCGGAGTTTTTGCTATTTATTATATAAAGCCTTGGTCAATTAGGTATGGTATCTCAGCCATATGTACCTTGGTAGCAACACTTATTTTCTTGACTGGTTCGTGTTCATATCACACTCATAGAGCACAAGGCAGCCCTCTCACTACTCTGTTTAGGGTGTTTGCGGCCTCTGCCTCCAAAATATTCAGAAGATGCCCGAGAGATGCCTCTCAGCTCTATGAAAAACTTGATGATGGTTATCAGATATCTCATACTCGTCGCCTCAGGTGTCTAGACAAGGCTGCGATTATATTAGCCACCCAACCTCTAGAGCAACAACAAAATAATAGGTGGAGGCTTTGCAGAGTCACAGAAGTGGAAGAAACCAAAAGCATCCTATGCATGATTCCCATATGCACAACCTTTATCCTAATGGGTGTTGTATCTTCTATTGGAAATACCTACTTCATCGAGCAAGCAACCCACATGAATCGCAAAGTTGGACGCATAAAGGTTCCTCTTCCAATCCTTTTATGGTTCTATGACCATGCCAAACAACAATTTGCCAAAATCTACTTCCAAATTGGAGGACTAACAAGGTATGGCCCCACAATTGGAATTGCTGTGTCAATGATATTTGCAATTCTATGTTGTATCACAGCTGCAAAAGTGGAGACAAGAAGGTTAGGCGCGGTTAAGAGACACGGTCTAATTGACAAGCCTGAGGAAACAATTCCCATGAGCATCTTCTGGTTGCTTCCACAGTTTCTTCTCCTCGGAGGCCTTGATGGGATTGCAGACAATAGCATTGGTTGGTTCTTCATTAATCAGGTCCCTCCATCCATGGCTCGGTACATGGTTCTATTTGCTATTGCTCTTTTTGGAGTGGGGATTGTGGGCAGTGTCTTGTCAGTTTATCTAGTGGGTGAGATAAGTGGAAGGGAAGGGAAACCCAGTTGGTTTCAAGAGACTCTAAACAAGAGTCGGTTGGATCAATATTATTGGACCTTGGCTGCATTGGCTGCAGCTAATCTTGTTCTGTATGTTTTGATGAGCATTTGGTATGCTTATAATGATTCAAGGTCGGAGGACGACGAAGCACCTGATTATGGGGAAACTGCTGAGCCATTTGATGATAATGTGCAATGCTGCTGCTGTTGTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

560

Amino Acids

62.39

Weight (kDa)

6.78

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1584
AccI GTMKAC 1 cut(s) 105
AccII CGCG 1 cut(s) 1205
AciI CCGC 3 cut(s) 65, 698, 1205
AclWI GGATC 2 cut(s) 514, 1512
AfaI GTAC 6 cut(s) 72, 83, 164, 183, 601, 1367
AfiI CCNNNNNNNGG 5 cut(s) 429, 536, 1127, 1362, 1622
AgsI TTSAA 3 cut(s) 476, 1478, 1593
AjuI GAANNNNNNNTTGG 2 cut(s) 1073, 1105
AleI CACNNNNGTG 1 cut(s) 887
AluBI AGCT 7 cut(s) 127, 389, 398, 507, 748, 1175, 1541
AluI AGCT 7 cut(s) 127, 389, 398, 507, 748, 1175, 1541
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 4 cut(s) 250, 1181, 1207, 1475
AlwI GGATC 2 cut(s) 514, 1512
Ama87I CYCGRG 1 cut(s) 730
AoxI GGCC 4 cut(s) 352, 699, 1117, 1294
AseI ATTAAT 1 cut(s) 1338
Asp700I GAANNNNTTC 1 cut(s) 1245
AspLEI GCGC 2 cut(s) 332, 1205
AspS9I GGNCC 3 cut(s) 1118, 1345, 1518
AsuHPI GGTGA 2 cut(s) 529, 1451
AvaI CYCGRG 1 cut(s) 730
AvaII GGWCC 2 cut(s) 1345, 1518
AxyI CCTNAGG 2 cut(s) 797, 1236
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 1 cut(s) 661
BccI CCATC 5 cut(s) 229, 508, 761, 1295, 1360
BceAI ACGGC 1 cut(s) 164
BcoDI GTCTC 4 cut(s) 250, 1181, 1207, 1475
BfaI CTAG 3 cut(s) 806, 842, 1433
BfmI CTRYAG 3 cut(s) 259, 531, 1536
BlpI GCTNAGC 2 cut(s) 444, 1635
Bme18I GGWCC 2 cut(s) 1345, 1518
BmeT110I CYCGRG 1 cut(s) 730
BmgT120I GGNCC 3 cut(s) 1118, 1345, 1518
BmiI GGNNCC 3 cut(s) 1029, 1120, 1347
BmrI ACTGGG 2 cut(s) 175, 1461
BmsI GCATC 5 cut(s) 202, 716, 727, 915, 1266
BmuI ACTGGG 2 cut(s) 175, 1461
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 232
Bpu10I CCTNAGC 1 cut(s) 503
Bpu1102I GCTNAGC 2 cut(s) 444, 1635
BsaBI GATNNNNATC 2 cut(s) 518, 771
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 7 cut(s) 99, 355, 566, 603, 1288, 1356, 1521
Bsc4I CCNNNNNNNGG 5 cut(s) 429, 536, 1127, 1362, 1622
Bse1I ACTGG 6 cut(s) 170, 188, 249, 299, 634, 1467
Bse21I CCTNAGG 2 cut(s) 797, 1236
Bse3DI GCAATG 3 cut(s) 93, 438, 1664
Bse8I GATNNNNATC 2 cut(s) 518, 771
BseDI CCNNGG 7 cut(s) 99, 355, 566, 603, 1288, 1356, 1521
BseGI GGATG 6 cut(s) 73, 99, 217, 240, 906, 1352
BseJI GATNNNNATC 2 cut(s) 518, 771
BseLI CCNNNNNNNGG 5 cut(s) 429, 536, 1127, 1362, 1622
BseMI GCAATG 3 cut(s) 93, 438, 1664
BseMII CTCAG 7 cut(s) 435, 494, 603, 758, 811, 1227, 1626
BseNI ACTGG 6 cut(s) 170, 188, 249, 299, 634, 1467
BseRI GAGGAG 1 cut(s) 1277
BseYI CCCAGC 1 cut(s) 308
Bsh1236I CGCG 1 cut(s) 1205
BshFI GGCC 4 cut(s) 354, 701, 1119, 1296
BsiHKAI GWGCWC 1 cut(s) 661
BsiHKCI CYCGRG 1 cut(s) 730
BsiSI CCGG 2 cut(s) 341, 537
BslFI GGGAC 2 cut(s) 512, 1331
BslI CCNNNNNNNGG 5 cut(s) 429, 536, 1127, 1362, 1622
BsmAI GTCTC 4 cut(s) 250, 1181, 1207, 1475
BsmFI GGGAC 2 cut(s) 512, 1331
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 4 cut(s) 354, 701, 1119, 1296
Bso31I GGTCTC 1 cut(s) 250
BsoBI CYCGRG 1 cut(s) 730
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 2 cut(s) 519, 1504
Bsp1720I GCTNAGC 2 cut(s) 444, 1635
Bsp19I CCATGG 2 cut(s) 99, 1356
BspACI CCGC 3 cut(s) 65, 698, 1205
BspANI GGCC 4 cut(s) 354, 701, 1119, 1296
BspCNI CTCAG 7 cut(s) 436, 495, 602, 757, 810, 1228, 1627
BspFNI CGCG 1 cut(s) 1205
BspLI GGNNCC 3 cut(s) 1029, 1120, 1347
BspMAI CTGCAG 1 cut(s) 1540
BspPI GGATC 2 cut(s) 514, 1512
BspTNI GGTCTC 1 cut(s) 250
BsrDI GCAATG 3 cut(s) 93, 438, 1664
BsrI ACTGG 6 cut(s) 170, 188, 249, 299, 634, 1467
BssECI CCNNGG 7 cut(s) 99, 355, 566, 603, 1288, 1356, 1521
BssMI GATC 2 cut(s) 519, 1504
BssNAI GTATAC 1 cut(s) 106
BssT1I CCWWGG 6 cut(s) 99, 355, 566, 603, 1356, 1521
Bst1107I GTATAC 1 cut(s) 106
Bst4CI ACNGT 4 cut(s) 45, 263, 1220, 1278
Bst6I CTCTTC 1 cut(s) 1038
BstAPI GCANNNNNTGC 3 cut(s) 440, 912, 1535
BstC8I GCNNGC 4 cut(s) 197, 268, 485, 990
BstDEI CTNAG 7 cut(s) 444, 503, 589, 744, 797, 1236, 1635
BstDSI CCRYGG 2 cut(s) 99, 1356
BstF5I GGATG 6 cut(s) 73, 99, 217, 240, 906, 1352
BstFNI CGCG 1 cut(s) 1205
BstHHI GCGC 2 cut(s) 332, 1205
BstKTI GATC 2 cut(s) 522, 1507
BstMAI GTCTC 4 cut(s) 250, 1181, 1207, 1475
BstMBI GATC 2 cut(s) 519, 1504
BstMWI GCNNNNNNNGC 9 cut(s) 83, 263, 327, 395, 440, 704, 912, 1532, 1535
BstNSI RCATGY 2 cut(s) 143, 487
BstSFI CTRYAG 3 cut(s) 259, 531, 1536
BstUI CGCG 1 cut(s) 1205
BstV2I GAAGAC 1 cut(s) 483
BstZ17I GTATAC 1 cut(s) 106
Bsu36I CCTNAGG 2 cut(s) 797, 1236
BsuRI GGCC 4 cut(s) 354, 701, 1119, 1296
BtgI CCRYGG 2 cut(s) 99, 1356
BtsCI GGATG 6 cut(s) 73, 99, 217, 240, 906, 1352
BtsI GCAGTG 1 cut(s) 1420
BtsIMutI CAGTG 3 cut(s) 195, 268, 1420
Cac8I GCNNGC 4 cut(s) 197, 268, 485, 990
CfoI GCGC 2 cut(s) 332, 1205
Cfr13I GGNCC 3 cut(s) 1118, 1345, 1518
CseI GACGC 1 cut(s) 1026
Csp6I GTAC 6 cut(s) 71, 82, 163, 182, 600, 1366
CviQI GTAC 6 cut(s) 71, 82, 163, 182, 600, 1366
DdeI CTNAG 7 cut(s) 444, 503, 589, 744, 797, 1236, 1635
DpnI GATC 2 cut(s) 521, 1506
DpnII GATC 2 cut(s) 519, 1504
Eam1104I CTCTTC 1 cut(s) 1038
EarI CTCTTC 1 cut(s) 1038
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 6 cut(s) 99, 355, 566, 603, 1356, 1521
Eco147I AGGCCT 1 cut(s) 1296
Eco31I GGTCTC 1 cut(s) 250
Eco32I GATATC 1 cut(s) 780
Eco47I GGWCC 2 cut(s) 1345, 1518
Eco81I CCTNAGG 2 cut(s) 797, 1236
Eco88I CYCGRG 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 1345
EcoRV GATATC 1 cut(s) 780
EcoT14I CCWWGG 6 cut(s) 99, 355, 566, 603, 1356, 1521
EcoT22I ATGCAT 2 cut(s) 6, 917
ErhI CCWWGG 6 cut(s) 99, 355, 566, 603, 1356, 1521
FaqI GGGAC 2 cut(s) 512, 1331
FauNDI CATATG 3 cut(s) 334, 596, 926
FblI GTMKAC 1 cut(s) 105
FokI GGATG 6 cut(s) 80, 106, 224, 247, 893, 1339
FspBI CTAG 3 cut(s) 806, 842, 1433
GlaI GCGC 2 cut(s) 331, 1204
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 1 cut(s) 232
HaeIII GGCC 4 cut(s) 354, 701, 1119, 1296
HapII CCGG 2 cut(s) 341, 537
HgaI GACGC 1 cut(s) 1026
HhaI GCGC 2 cut(s) 332, 1205
Hin6I GCGC 2 cut(s) 330, 1203
HinP1I GCGC 2 cut(s) 330, 1203
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 7 cut(s) 458, 879, 919, 1003, 1483, 1495, 1589
HpaII CCGG 2 cut(s) 341, 537
HphI GGTGA 2 cut(s) 529, 1451
Hpy166II GTNNAC 3 cut(s) 82, 106, 291
Hpy188I TCNGA 4 cut(s) 722, 777, 1292, 1600
Hpy188III TCNNGA 6 cut(s) 502, 511, 625, 806, 842, 1478
Hpy8I GTNNAC 3 cut(s) 82, 106, 291
Hpy99I CGWCG 2 cut(s) 795, 1610
HpyAV CCTTC 3 cut(s) 1188, 1446, 1451
HpyCH4III ACNGT 4 cut(s) 45, 263, 1220, 1278
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 263, 327, 395, 440, 704, 912, 1532, 1535
HpyF3I CTNAG 7 cut(s) 444, 503, 589, 744, 797, 1236, 1635
HspAI GCGC 2 cut(s) 330, 1203
Kzo9I GATC 2 cut(s) 519, 1504
LmnI GCTCC 1 cut(s) 40
LweI GCATC 5 cut(s) 202, 716, 727, 915, 1266
MaeI CTAG 3 cut(s) 806, 842, 1433
MaeIII GTNAC 3 cut(s) 224, 491, 880
MalI GATC 2 cut(s) 521, 1506
MboI GATC 2 cut(s) 519, 1504
MboII GAAGA 8 cut(s) 488, 735, 905, 951, 1025, 1252, 1274, 1324
MfeI CAATTG 2 cut(s) 150, 1125
MhlI GDGCHC 1 cut(s) 661
MlyI GAGTC 4 cut(s) 452, 888, 1477, 1504
MmeI TCCRAC 3 cut(s) 994, 1482, 1578
Mph1103I ATGCAT 2 cut(s) 6, 917
MroXI GAANNNNTTC 1 cut(s) 1245
MseI TTAA 5 cut(s) 18, 110, 419, 1209, 1338
MslI CAYNNNNRTG 1 cut(s) 887
MspA1I CMGCKG 1 cut(s) 1175
MspI CCGG 2 cut(s) 341, 537
MunI CAATTG 2 cut(s) 150, 1125
Mva1269I GAATGC 1 cut(s) 434
MvnI CGCG 1 cut(s) 1205
MwoI GCNNNNNNNGC 9 cut(s) 83, 263, 327, 395, 440, 704, 912, 1532, 1535
NcoI CCATGG 2 cut(s) 99, 1356
NdeI CATATG 3 cut(s) 334, 596, 926
NdeII GATC 2 cut(s) 519, 1504
NlaIV GGNNCC 3 cut(s) 1029, 1120, 1347
NmuCI GTSAC 2 cut(s) 491, 880
NsiI ATGCAT 2 cut(s) 6, 917
NspI RCATGY 2 cut(s) 143, 487
OliI CACNNNNGTG 1 cut(s) 887
PaeI GCATGC 1 cut(s) 487
PceI AGGCCT 1 cut(s) 1296
PctI GAATGC 1 cut(s) 434
PdmI GAANNNNTTC 1 cut(s) 1245
PfeI GAWTC 3 cut(s) 919, 1003, 1589
PflFI GACNNNGTC 1 cut(s) 1218
PleI GAGTC 4 cut(s) 452, 887, 1477, 1503
PpsI GAGTC 4 cut(s) 452, 887, 1477, 1503
PpuMI RGGWCCY 1 cut(s) 1345
PshBI ATTAAT 1 cut(s) 1338
PsiI TTATAA 1 cut(s) 1584
Psp5II RGGWCCY 1 cut(s) 1345
PspFI CCCAGC 1 cut(s) 308
PspN4I GGNNCC 3 cut(s) 1029, 1120, 1347
PspPI GGNCC 3 cut(s) 1118, 1345, 1518
PspPPI RGGWCCY 1 cut(s) 1345
PstI CTGCAG 1 cut(s) 1540
PsyI GACNNNGTC 1 cut(s) 1218
PvuII CAGCTG 1 cut(s) 1175
RsaI GTAC 6 cut(s) 72, 83, 164, 183, 601, 1367
RsaNI GTAC 6 cut(s) 71, 82, 163, 182, 600, 1366
RseI CAYNNNNRTG 1 cut(s) 887
SaqAI TTAA 5 cut(s) 18, 110, 419, 1209, 1338
Sau3AI GATC 2 cut(s) 519, 1504
Sau96I GGNCC 3 cut(s) 1118, 1345, 1518
SchI GAGTC 4 cut(s) 452, 888, 1477, 1504
SduI GDGCHC 1 cut(s) 661
SfaNI GCATC 5 cut(s) 202, 716, 727, 915, 1266
SfcI CTRYAG 3 cut(s) 259, 531, 1536
SinI GGWCC 2 cut(s) 1345, 1518
SmiMI CAYNNNNRTG 1 cut(s) 887
SphI GCATGC 1 cut(s) 487
SseBI AGGCCT 1 cut(s) 1296
SsiI CCGC 3 cut(s) 65, 698, 1205
SspI AATATT 2 cut(s) 717, 1511
SspMI CTAG 3 cut(s) 806, 842, 1433
StuI AGGCCT 1 cut(s) 1296
StyI CCWWGG 6 cut(s) 99, 355, 566, 603, 1356, 1521
TaaI ACNGT 4 cut(s) 45, 263, 1220, 1278
TaqI TCGA 1 cut(s) 984
TatI WGTACW 2 cut(s) 70, 162
TauI GCSGC 1 cut(s) 701
TfiI GAWTC 3 cut(s) 919, 1003, 1589
Tru1I TTAA 5 cut(s) 18, 110, 419, 1209, 1338
Tru9I TTAA 5 cut(s) 18, 110, 419, 1209, 1338
TscAI CASTG 3 cut(s) 195, 268, 1420
TseFI GTSAC 2 cut(s) 491, 880
Tsp45I GTSAC 2 cut(s) 491, 880
TspDTI ATGAA 7 cut(s) 208, 427, 630, 768, 970, 1016, 1324
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 3 cut(s) 195, 268, 1420
Tth111I GACNNNGTC 1 cut(s) 1218
VpaK11BI GGWCC 2 cut(s) 1345, 1518
VspI ATTAAT 1 cut(s) 1338
XbaI TCTAGA 2 cut(s) 805, 841
XceI RCATGY 2 cut(s) 143, 487
XcmI CCANNNNNNNNNTGG 2 cut(s) 601, 1044
XmiI GTMKAC 1 cut(s) 105
XmnI GAANNNNTTC 1 cut(s) 1245
XspI CTAG 3 cut(s) 806, 842, 1433
Zsp2I ATGCAT 2 cut(s) 6, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.