Rroxscaffold_1G00019490

POT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001179
Physical Location & Seq
Forward (+)
23814556 .. 23818546
3991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019490.1

Sequence Viewer

Length: 1452 bp
ATGGCCTCATCTAATACTCGGATGATTCAGATGATGGTTTGGGTGAAAATCGCTGTTCTGCAGAAGACGTATTTACTGACTGGATATGCAATGAGCACAATGATTACATACTTGACTAGTGTATGGAAGTTAAATCTCACAGATGCTGCCGCTGTCGTCAATGTGTATGCCGGCGCAGTGGCCGTAATGCCACTGGGCATGCTTTTTGTTGTTGATACTGTAATCGGGGTTTTCCTCTTGACCATGTCAACACCACCAGTTCTTTATAGGGCAACAGGAACTTGTGGCGTATTTGAGGCCGAGTGCATTGGCAAAGTACAAAAGATTCTCTTTTACACAGCCTTAACTCTAATAGCTCTGGGGGCCTCTGGTCGCTTTATGGTTCATGCATTCAGAGAAGAGCAGTTGATCAAGCCACTATTAGAAGGCGCCAAAAAAATGAAGTTCTTTGCTCTGTCAGTGTGCCCTAAGAAAGTAAGGTTGCTGTCATTTCAAATTCTGGTCTCCACCCTGGTACTCGGGACAACTGCTTATTTAAAGCCTTGGTCGCTTAGGTTTGGGTTCCCTGCTATATTTACCTTGCTTGCAACAATTACTTTATTGACAAGTACTATACATCCACACAAAGCACAAAGGAACCTTTTCACCAGGGAAGAAATCAGAAGCATCTTGCGCATGAGTCCTATATGTATTTTCCTTATTCCAATTGGTGTTGTATCGTCTCTTGGAAATACTTATTTCACTGAACAAGCAAGCAAGATGAATCACAAGGTTGGATCCTTAAAGGTTTCTGTTCTTTTCCTTATAGTGCCCCGAACTGCATTAGTGGAAAACAGAAGGTTGGGCGTGGTTAAGAGACATGGTTTACTTAACAAGCCCAATGAGACAGTTCCCATGACCATGTTCTGGTTGCTTCCACAGTTTCTTCTCGTTGGAGGCGTTTCTGGGCTTTCCGAGAAGTGCATTGCTCTTTTCTACACTGATGAGGTGCTTCACTGGCCGAAAATAGAAAAAGATGTCGATATACAAGCAAAGGACAAACCCAATTCCAAAGAACAAAACATTAAGTCCCCCATGGAACAGGCAAACGAAACACTCAAGCCTACAGAAAAGAATGATGAGGACCCCAATCAGGCAAAACAAGAAACAAAGAGTACAGAAAAAGATGGTGAGGGGCACATTGATCCCGAAAGGGCAAAACAAATGTCCAAGCGTCAATACATAGAGTTTTTTGTTGATGCTCTTGAGGGAATGGGAAATATAGGCAGTGTGGTGTTGGTCTATGTAGTGGGTGAAATAAAACCTAATTGGTTCCAAGAATCGGTAAACCGAAGTCATTTGGATAATTATTACTGGACTTTGGCTGCATTGACTGCAGCTAATTTGATATTGTTTATTCCAGTAATATTCTACCTGTTGAGGTCAAAGAAATTCACTGCATTGTTTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

483

Amino Acids

54.18

Weight (kDa)

9.43

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 428
AccB7I CCANNNNNTGG 1 cut(s) 906
AciI CCGC 1 cut(s) 150
AclWI GGATC 3 cut(s) 771, 784, 1178
AcoI YGGCCR 2 cut(s) 180, 998
AcsI RAATTY 2 cut(s) 495, 1430
AcyI GRCGYC 1 cut(s) 429
AfaI GTAC 4 cut(s) 318, 516, 610, 1156
AfiI CCNNNNNNNGG 3 cut(s) 906, 1132, 1321
AgsI TTSAA 1 cut(s) 494
AhlI ACTAGT 1 cut(s) 116
AjnI CCWGG 2 cut(s) 510, 647
AluBI AGCT 2 cut(s) 356, 1379
AluI AGCT 2 cut(s) 356, 1379
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 4 cut(s) 508, 726, 850, 878
AlwI GGATC 3 cut(s) 771, 784, 1178
AlwNI CAGNNNCTG 1 cut(s) 146
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 5 cut(s) 3, 180, 297, 363, 998
ApeKI GCWGC 3 cut(s) 146, 1364, 1376
ApoI RAATTY 2 cut(s) 495, 1430
ArsI GACNNNNNNTTYG 2 cut(s) 1190, 1222
Asp700I GAANNNNTTC 1 cut(s) 641
AspLEI GCGC 3 cut(s) 176, 431, 675
AspS9I GGNCC 2 cut(s) 363, 1123
AsuHPI GGTGA 4 cut(s) 55, 637, 1181, 1304
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 1 cut(s) 1123
BaeGI GKGCMC 3 cut(s) 467, 813, 1179
BamHI GGATCC 1 cut(s) 776
BanI GGYRCC 1 cut(s) 428
BbsI GAAGAC 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 133, 1351, 1388
BccI CCATC 2 cut(s) 28, 1160
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 2 cut(s) 512, 649
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 4 cut(s) 508, 726, 850, 878
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 3 cut(s) 59, 1104, 1374
BfoI RGCGCY 1 cut(s) 432
BisI GCNGC 4 cut(s) 147, 150, 1365, 1377
BlsI GCNGC 4 cut(s) 148, 151, 1366, 1378
BmcAI AGTACT 1 cut(s) 610
Bme1390I CCNGG 2 cut(s) 512, 649
Bme18I GGWCC 1 cut(s) 1123
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 2 cut(s) 363, 1123
BmiI GGNNCC 7 cut(s) 364, 430, 563, 638, 778, 1125, 1313
BmrFI CCNGG 2 cut(s) 512, 649
BmrI ACTGGG 1 cut(s) 203
BmsI GCATC 3 cut(s) 133, 675, 1228
BmuI ACTGGG 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 71
Bpu10I CCTNAGC 1 cut(s) 551
BpuEI CTTGAG 2 cut(s) 1082, 1265
BsaHI GRCGYC 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 508
BsaJI CCNNGG 4 cut(s) 510, 542, 648, 1074
Bsc4I CCNNNNNNNGG 3 cut(s) 906, 1132, 1321
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 6 cut(s) 85, 198, 257, 1001, 1358, 1400
Bse3DI GCAATG 2 cut(s) 96, 963
BseBI CCWGG 2 cut(s) 512, 649
BseDI CCNNGG 4 cut(s) 510, 542, 648, 1074
BseGI GGATG 2 cut(s) 27, 616
BseLI CCNNNNNNNGG 3 cut(s) 906, 1132, 1321
BseMI GCAATG 2 cut(s) 96, 963
BseNI ACTGG 6 cut(s) 85, 198, 257, 1001, 1358, 1400
BseSI GKGCMC 3 cut(s) 467, 813, 1179
BseXI GCAGC 3 cut(s) 133, 1351, 1388
BshFI GGCC 5 cut(s) 5, 182, 299, 365, 1000
BshNI GGYRCC 1 cut(s) 428
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 1 cut(s) 171
BslFI GGGAC 2 cut(s) 535, 1054
BslI CCNNNNNNNGG 3 cut(s) 906, 1132, 1321
BsmAI GTCTC 4 cut(s) 508, 726, 850, 878
BsmBI CGTCTC 1 cut(s) 726
BsmFI GGGAC 2 cut(s) 535, 1054
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 5 cut(s) 5, 182, 299, 365, 1000
Bso31I GGTCTC 1 cut(s) 508
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 4 cut(s) 98, 467, 813, 1179
Bsp143I GATC 3 cut(s) 408, 776, 1183
Bsp19I CCATGG 1 cut(s) 1074
BspACI CCGC 1 cut(s) 150
BspANI GGCC 5 cut(s) 5, 182, 299, 365, 1000
BspLI GGNNCC 7 cut(s) 364, 430, 563, 638, 778, 1125, 1313
BspMAI CTGCAG 2 cut(s) 63, 1378
BspPI GGATC 3 cut(s) 771, 784, 1178
BspQI GCTCTTC 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 508
BsrDI GCAATG 2 cut(s) 96, 963
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 6 cut(s) 85, 198, 257, 1001, 1358, 1400
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 4 cut(s) 510, 542, 648, 1074
BssMI GATC 3 cut(s) 408, 776, 1183
BssNI GRCGYC 1 cut(s) 429
BssT1I CCWWGG 2 cut(s) 542, 1074
Bst2UI CCWGG 2 cut(s) 512, 649
Bst4CI ACNGT 3 cut(s) 220, 889, 921
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 429
BstAPI GCANNNNNTGC 2 cut(s) 1373, 1445
BstC8I GCNNGC 4 cut(s) 172, 200, 585, 754
BstDEI CTNAG 2 cut(s) 468, 551
BstDSI CCRYGG 1 cut(s) 1074
BstF5I GGATG 2 cut(s) 27, 616
BstH2I RGCGCY 1 cut(s) 432
BstHHI GCGC 3 cut(s) 176, 431, 675
BstKTI GATC 3 cut(s) 411, 779, 1186
BstMAI GTCTC 4 cut(s) 508, 726, 850, 878
BstMBI GATC 3 cut(s) 408, 776, 1183
BstMWI GCNNNNNNNGC 6 cut(s) 362, 547, 672, 997, 1373, 1445
BstNI CCWGG 2 cut(s) 512, 649
BstNSI RCATGY 1 cut(s) 202
BstSCI CCNGG 2 cut(s) 510, 647
BstSFI CTRYAG 3 cut(s) 59, 1104, 1374
BstSLI GKGCMC 3 cut(s) 467, 813, 1179
BstV1I GCAGC 3 cut(s) 133, 1351, 1388
BstV2I GAAGAC 1 cut(s) 71
BstX2I RGATCY 1 cut(s) 776
BstYI RGATCY 1 cut(s) 776
BsuRI GGCC 5 cut(s) 5, 182, 299, 365, 1000
BtgI CCRYGG 1 cut(s) 1074
BtsCI GGATG 2 cut(s) 27, 616
BtsI GCAGTG 3 cut(s) 183, 1273, 1434
BtsIMutI CAGTG 8 cut(s) 183, 191, 465, 741, 978, 994, 1273, 1434
Cac8I GCNNGC 4 cut(s) 172, 200, 585, 754
CaiI CAGNNNCTG 1 cut(s) 146
CfoI GCGC 3 cut(s) 176, 431, 675
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 2 cut(s) 363, 1123
CseI GACGC 1 cut(s) 1202
Csp6I GTAC 4 cut(s) 317, 515, 609, 1155
CviAII CATG 8 cut(s) 199, 244, 386, 676, 860, 895, 901, 1075
CviQI GTAC 4 cut(s) 317, 515, 609, 1155
DdeI CTNAG 2 cut(s) 468, 551
DinI GGCGCC 1 cut(s) 430
DpnI GATC 3 cut(s) 410, 778, 1185
DpnII GATC 3 cut(s) 408, 776, 1183
DraI TTTAAA 1 cut(s) 537
EaeI YGGCCR 2 cut(s) 180, 998
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco130I CCWWGG 2 cut(s) 542, 1074
Eco31I GGTCTC 1 cut(s) 508
Eco47I GGWCC 1 cut(s) 1123
Eco88I CYCGRG 1 cut(s) 518
EcoO109I RGGNCCY 2 cut(s) 363, 1123
EcoRII CCWGG 2 cut(s) 510, 647
EcoT14I CCWWGG 2 cut(s) 542, 1074
EcoT22I ATGCAT 1 cut(s) 391
EgeI GGCGCC 1 cut(s) 430
EheI GGCGCC 1 cut(s) 430
ErhI CCWWGG 2 cut(s) 542, 1074
Esp3I CGTCTC 1 cut(s) 726
FaeI CATG 8 cut(s) 202, 247, 389, 679, 863, 898, 904, 1078
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 2 cut(s) 535, 1054
FatI CATG 8 cut(s) 198, 243, 385, 675, 859, 894, 900, 1074
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 4 cut(s) 147, 150, 1365, 1377
FokI GGATG 2 cut(s) 34, 603
Fsp4HI GCNGC 4 cut(s) 147, 150, 1365, 1377
FspBI CTAG 1 cut(s) 117
FspI TGCGCA 1 cut(s) 674
GlaI GCGC 3 cut(s) 175, 430, 674
GluI GCNGC 4 cut(s) 147, 150, 1365, 1377
HaeII RGCGCY 1 cut(s) 432
HaeIII GGCC 5 cut(s) 5, 182, 299, 365, 1000
HapII CCGG 1 cut(s) 171
HgaI GACGC 1 cut(s) 1202
HhaI GCGC 3 cut(s) 176, 431, 675
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 8 cut(s) 202, 247, 389, 679, 863, 898, 904, 1078
Hin6I GCGC 3 cut(s) 174, 429, 673
HinP1I GCGC 3 cut(s) 174, 429, 673
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
HinfI GANTC 5 cut(s) 25, 325, 679, 763, 1319
HpaII CCGG 1 cut(s) 171
HphI GGTGA 4 cut(s) 55, 637, 1181, 1304
Hpy166II GTNNAC 3 cut(s) 249, 866, 1327
Hpy188I TCNGA 5 cut(s) 21, 30, 395, 662, 955
Hpy188III TCNNGA 4 cut(s) 238, 520, 1187, 1244
Hpy8I GTNNAC 3 cut(s) 249, 866, 1327
HpyAV CCTTC 2 cut(s) 419, 831
HpyCH4III ACNGT 3 cut(s) 220, 889, 921
HpyCH4IV ACGT 1 cut(s) 68
HpyF10VI GCNNNNNNNGC 6 cut(s) 362, 547, 672, 997, 1373, 1445
HpyF3I CTNAG 2 cut(s) 468, 551
HpySE526I ACGT 1 cut(s) 68
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 8 cut(s) 202, 247, 389, 679, 863, 898, 904, 1078
HspAI GCGC 3 cut(s) 174, 429, 673
KasI GGCGCC 1 cut(s) 428
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 3 cut(s) 408, 776, 1183
LguI GCTCTTC 1 cut(s) 393
Lsp1109I GCAGC 3 cut(s) 133, 1351, 1388
LweI GCATC 3 cut(s) 133, 675, 1228
MaeI CTAG 1 cut(s) 117
MaeII ACGT 1 cut(s) 68
MalI GATC 3 cut(s) 410, 778, 1185
MboI GATC 3 cut(s) 408, 776, 1183
MboII GAAGA 4 cut(s) 76, 410, 665, 917
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 1 cut(s) 776
MhlI GDGCHC 4 cut(s) 98, 467, 813, 1179
MluCI AATT 8 cut(s) 495, 591, 705, 1045, 1306, 1345, 1381, 1430
Mly113I GGCGCC 1 cut(s) 429
MlyI GAGTC 1 cut(s) 688
MmeI TCCRAC 2 cut(s) 754, 913
Mph1103I ATGCAT 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 170
MroXI GAANNNNTTC 1 cut(s) 641
MseI TTAA 7 cut(s) 131, 344, 536, 782, 852, 870, 1065
MslI CAYNNNNRTG 1 cut(s) 899
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 2 cut(s) 512, 649
MunI CAATTG 1 cut(s) 705
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 2 cut(s) 512, 649
MwoI GCNNNNNNNGC 6 cut(s) 362, 547, 672, 997, 1373, 1445
NaeI GCCGGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 429
NcoI CCATGG 1 cut(s) 1074
NdeII GATC 3 cut(s) 408, 776, 1183
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 8 cut(s) 202, 247, 389, 679, 863, 898, 904, 1078
NlaIV GGNNCC 7 cut(s) 364, 430, 563, 638, 778, 1125, 1313
NmeAIII GCCGAG 1 cut(s) 325
NsbI TGCGCA 1 cut(s) 674
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 1 cut(s) 202
PaeI GCATGC 1 cut(s) 202
PciSI GCTCTTC 1 cut(s) 393
PcsI WCGNNNNNNNCGW 1 cut(s) 936
PctI GAATGC 1 cut(s) 389
PdiI GCCGGC 1 cut(s) 172
PdmI GAANNNNTTC 1 cut(s) 641
PfeI GAWTC 4 cut(s) 25, 325, 763, 1319
PflFI GACNNNGTC 1 cut(s) 244
PflMI CCANNNNNTGG 1 cut(s) 906
PkrI GCNGC 4 cut(s) 148, 151, 1366, 1378
PleI GAGTC 1 cut(s) 687
PluTI GGCGCC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 687
PpuMI RGGWCCY 1 cut(s) 1123
Psp5II RGGWCCY 1 cut(s) 1123
Psp6I CCWGG 2 cut(s) 510, 647
PspGI CCWGG 2 cut(s) 510, 647
PspN4I GGNNCC 7 cut(s) 364, 430, 563, 638, 778, 1125, 1313
PspPI GGNCC 2 cut(s) 363, 1123
PspPPI RGGWCCY 1 cut(s) 1123
PstI CTGCAG 2 cut(s) 63, 1378
PstNI CAGNNNCTG 1 cut(s) 146
PsuI RGATCY 1 cut(s) 776
PsyI GACNNNGTC 1 cut(s) 244
RsaI GTAC 4 cut(s) 318, 516, 610, 1156
RsaNI GTAC 4 cut(s) 317, 515, 609, 1155
RseI CAYNNNNRTG 1 cut(s) 899
SapI GCTCTTC 1 cut(s) 393
SaqAI TTAA 7 cut(s) 131, 344, 536, 782, 852, 870, 1065
SatI GCNGC 4 cut(s) 147, 150, 1365, 1377
Sau3AI GATC 3 cut(s) 408, 776, 1183
Sau96I GGNCC 2 cut(s) 363, 1123
ScaI AGTACT 1 cut(s) 610
SchI GAGTC 1 cut(s) 688
ScrFI CCNGG 2 cut(s) 512, 649
SduI GDGCHC 4 cut(s) 98, 467, 813, 1179
SfaNI GCATC 3 cut(s) 133, 675, 1228
SfcI CTRYAG 3 cut(s) 59, 1104, 1374
SfoI GGCGCC 1 cut(s) 430
SinI GGWCC 1 cut(s) 1123
SmiMI CAYNNNNRTG 1 cut(s) 899
SmlI CTYRAG 2 cut(s) 1097, 1244
SmoI CTYRAG 2 cut(s) 1097, 1244
SpeI ACTAGT 1 cut(s) 116
SphI GCATGC 1 cut(s) 202
Sse9I AATT 8 cut(s) 495, 591, 705, 1045, 1306, 1345, 1381, 1430
SsiI CCGC 1 cut(s) 150
SspDI GGCGCC 1 cut(s) 428
SspI AATATT 1 cut(s) 1407
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 2 cut(s) 510, 647
StyI CCWWGG 2 cut(s) 542, 1074
TaaI ACNGT 3 cut(s) 220, 889, 921
TaiI ACGT 1 cut(s) 71
TaqI TCGA 1 cut(s) 1020
TasI AATT 8 cut(s) 495, 591, 705, 1045, 1306, 1345, 1381, 1430
TatI WGTACW 3 cut(s) 316, 608, 1154
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 4 cut(s) 25, 325, 763, 1319
Tru1I TTAA 7 cut(s) 131, 344, 536, 782, 852, 870, 1065
Tru9I TTAA 7 cut(s) 131, 344, 536, 782, 852, 870, 1065
TscAI CASTG 8 cut(s) 183, 198, 465, 748, 985, 1001, 1273, 1441
TseI GCWGC 3 cut(s) 146, 1364, 1376
TspDTI ATGAA 3 cut(s) 374, 455, 776
TspRI CASTG 8 cut(s) 183, 198, 465, 748, 985, 1001, 1273, 1441
Tth111I GACNNNGTC 1 cut(s) 244
Van91I CCANNNNNTGG 1 cut(s) 906
VpaK11BI GGWCC 1 cut(s) 1123
XapI RAATTY 2 cut(s) 495, 1430
XceI RCATGY 1 cut(s) 202
XmnI GAANNNNTTC 1 cut(s) 641
XspI CTAG 1 cut(s) 117
ZrmI AGTACT 1 cut(s) 610
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.