FvH4_1g22630

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001539
Physical Location & Seq
Reverse (-)
14557979 .. 14559828
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g22630.t1

Sequence Viewer

Length: 1203 bp
ATGACTAAGCTTACAAGTTGTTTTGCGATAGTTGTGTTCATTTCTCTACATATTCTATACCAATCATCTGCCTCTGGTGATCAGAACAGTACAAGTACATATAATGTTCTCAAGTTTGGAGCGAAGCGCAATGGTGTAACCGACTCAACCCAAGCTTTTCTCGAAACCTGGTCTGCAGCATGTGGCACCTCTTCAGATTCCAGTGAAATATATGTACCAAAGGGGAGGTATTTGCTTGGCGCTATGGCCTTTAAAGGTGACTGCAACTCACATGTCACTTTTCGGATTGATGGAACACTCGTGGCTTCGCCTGATTACCGAGTATTAGGCCAAGCGAACAACTGGTTCAGCTTTGAAGGAGTTAGCGGGGTGACAATAATCGGAGGGGCACTTGATGCCAAGGGCTCCGCCCTGTGGGCTTGCAAGTTAAGCACTAAAACCACTAGCTGTCCTAATGGAGCCACGACTTTGAGTTTCACAAACTCCAAGGACATTAGAGTTGAAGGACTAATGTCATTGAACAGCCAAATGTTTCACATTGTGATAAACGGCTGCCAAGATGTACTCATTCAAGGCGTCAAAGTCATTGCTGCTGGCAATAGCCCAAACACCGACGGCATCCATGTCCAGTTCTCAAAAAATGTTGCAATCTTCGACACCTCCATCAAAACTGGGGACGATTGTGTCTCTATTGGCCCCGGCACCAAGGACTTGTGGATGGAACGCATATTTTGCGGACCTGGCCATGGCATTAGCATTGGGAGCCTGGCCAAAGATTTGGTAGAGGAAGGAGTCCAAAATGTAACACTCAAAAATGCGATTTTTAAGGGTACCCAAAATGGTCTGAGGATCAAATCCTGGGCAAGACCCAGCACAGGGTTTGTACAAGGTGTTCAATTCCTTGACGTCAAAATGATTAATGTCCAAAACCCCATTATAATCGACCAAAATTACTGTCCACACAACCTCAACTGTCCTGGTCAGGTATCAGGTGTCAAAGTTAGTGATGTACTGTACCAGAACATTAGAGGAACATCAGCAACAGCAGTCGCAATAAAACTTGATTGTAGTGCAGAAAATCCATGCAGCGGAGTAAGACTGGAAAACGTGAATTTGACTTGCCGGAAGCAAGCGATTCAATCATATTGTACGAATGTAAATGGAAAGAGTGTTGGCACTGTGCAAACAAGCAGTTGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

401

Amino Acids

42.66

Weight (kDa)

8.44

Isoelectric Point (pI)

24.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 35 - 79 7.3e-06 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 66 - 388 1.7e-86 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 938
AasI GACNNNNNNGTC 1 cut(s) 683
AatII GACGTC 1 cut(s) 909
Acc65I GGTACC 1 cut(s) 830
AccB1I GGYRCC 3 cut(s) 185, 701, 830
AciI CCGC 4 cut(s) 366, 408, 735, 1089
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 2 cut(s) 742, 768
AcsI RAATTY 1 cut(s) 1111
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 2 cut(s) 576, 906
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 9 cut(s) 91, 97, 216, 564, 832, 885, 1011, 1016, 1150
AfiI CCNNNNNNNGG 5 cut(s) 414, 746, 875, 876, 1088
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 6 cut(s) 356, 503, 520, 572, 896, 1139
AjnI CCWGG 5 cut(s) 167, 739, 765, 857, 976
AluBI AGCT 4 cut(s) 10, 155, 351, 447
AluI AGCT 4 cut(s) 10, 155, 351, 447
Alw26I GTCTC 1 cut(s) 691
AlwI GGATC 1 cut(s) 857
AoxI GGCC 5 cut(s) 246, 328, 694, 742, 768
ApeKI GCWGC 4 cut(s) 176, 552, 590, 1086
ApoI RAATTY 1 cut(s) 1111
ArsI GACNNNNNNTTYG 2 cut(s) 940, 972
AseI ATTAAT 1 cut(s) 918
Asp718I GGTACC 1 cut(s) 830
AspLEI GCGC 2 cut(s) 129, 242
AspS9I GGNCC 2 cut(s) 695, 737
AsuC2I CCSGG 1 cut(s) 699
AsuHPI GGTGA 3 cut(s) 89, 269, 382
AvaII GGWCC 1 cut(s) 737
BaeGI GKGCMC 1 cut(s) 391
BalI TGGCCA 2 cut(s) 744, 770
BanI GGYRCC 3 cut(s) 185, 701, 830
BanII GRGCYC 1 cut(s) 407
BauI CACGAG 1 cut(s) 299
BbvI GCAGC 4 cut(s) 188, 539, 577, 1098
BccI CCATC 3 cut(s) 284, 671, 712
BceAI ACGGC 2 cut(s) 565, 631
BciT130I CCWGG 5 cut(s) 169, 741, 767, 859, 978
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 1 cut(s) 699
BcoDI GTCTC 1 cut(s) 691
BfaI CTAG 1 cut(s) 444
BfmI CTRYAG 1 cut(s) 174
BfoI RGCGCY 1 cut(s) 243
BglI GCCNNNNNGGC 1 cut(s) 416
BisI GCNGC 4 cut(s) 177, 553, 591, 1087
BlsI GCNGC 4 cut(s) 178, 554, 592, 1088
Bme1390I CCNGG 6 cut(s) 169, 699, 741, 767, 859, 978
Bme18I GGWCC 1 cut(s) 737
BmgT120I GGNCC 2 cut(s) 695, 737
BmiI GGNNCC 7 cut(s) 187, 406, 460, 697, 703, 764, 832
BmrFI CCNGG 6 cut(s) 169, 699, 741, 767, 859, 978
BmrI ACTGGG 1 cut(s) 681
BmsI GCATC 2 cut(s) 385, 627
BmuI ACTGGG 1 cut(s) 681
BoxI GACNNNNGTC 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 699
BsaHI GRCGYC 2 cut(s) 576, 906
BsaJI CCNNGG 6 cut(s) 399, 486, 697, 705, 745, 858
Bsc4I CCNNNNNNNGG 5 cut(s) 414, 746, 875, 876, 1088
Bse1I ACTGG 5 cut(s) 201, 347, 628, 676, 1104
Bse3DI GCAATG 2 cut(s) 136, 585
BseBI CCWGG 5 cut(s) 169, 741, 767, 859, 978
BseDI CCNNGG 6 cut(s) 399, 486, 697, 705, 745, 858
BseGI GGATG 2 cut(s) 618, 723
BseLI CCNNNNNNNGG 5 cut(s) 414, 746, 875, 876, 1088
BseMI GCAATG 2 cut(s) 136, 585
BseMII CTCAG 1 cut(s) 836
BseNI ACTGG 5 cut(s) 201, 347, 628, 676, 1104
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 4 cut(s) 188, 539, 577, 1098
BseYI CCCAGC 1 cut(s) 869
BsgI GTGCAG 1 cut(s) 1092
BshFI GGCC 5 cut(s) 248, 330, 696, 744, 770
BshNI GGYRCC 3 cut(s) 185, 701, 830
BsiSI CCGG 2 cut(s) 699, 1123
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 5 cut(s) 414, 746, 875, 876, 1088
BsmAI GTCTC 1 cut(s) 691
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 5 cut(s) 248, 330, 696, 744, 770
Bsp1286I GDGCHC 2 cut(s) 391, 407
Bsp1407I TGTACA 1 cut(s) 883
Bsp143I GATC 2 cut(s) 79, 849
Bsp19I CCATGG 1 cut(s) 745
BspACI CCGC 4 cut(s) 366, 408, 735, 1089
BspANI GGCC 5 cut(s) 248, 330, 696, 744, 770
BspCNI CTCAG 1 cut(s) 837
BspLI GGNNCC 7 cut(s) 187, 406, 460, 697, 703, 764, 832
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 1 cut(s) 857
BspT107I GGYRCC 3 cut(s) 185, 701, 830
BsrDI GCAATG 2 cut(s) 136, 585
BsrGI TGTACA 1 cut(s) 883
BsrI ACTGG 5 cut(s) 201, 347, 628, 676, 1104
BssECI CCNNGG 6 cut(s) 399, 486, 697, 705, 745, 858
BssMI GATC 2 cut(s) 79, 849
BssNI GRCGYC 2 cut(s) 576, 906
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 4 cut(s) 399, 486, 705, 745
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 5 cut(s) 169, 741, 767, 859, 978
Bst4CI ACNGT 5 cut(s) 89, 956, 974, 1014, 1180
Bst6I CTCTTC 1 cut(s) 196
BstACI GRCGYC 2 cut(s) 576, 906
BstAPI GCANNNNNTGC 2 cut(s) 395, 732
BstAUI TGTACA 1 cut(s) 883
BstC8I GCNNGC 3 cut(s) 421, 595, 1131
BstDEI CTNAG 2 cut(s) 6, 845
BstDSI CCRYGG 1 cut(s) 745
BstF5I GGATG 2 cut(s) 618, 723
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 2 cut(s) 129, 242
BstKTI GATC 2 cut(s) 82, 852
BstMAI GTCTC 1 cut(s) 691
BstMBI GATC 2 cut(s) 79, 849
BstMWI GCNNNNNNNGC 6 cut(s) 395, 416, 429, 732, 741, 762
BstNI CCWGG 5 cut(s) 169, 741, 767, 859, 978
BstNSI RCATGY 2 cut(s) 183, 275
BstPAI GACNNNNGTC 1 cut(s) 511
BstSCI CCNGG 6 cut(s) 167, 697, 739, 765, 857, 976
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 4 cut(s) 188, 539, 577, 1098
BstXI CCANNNNNNTGG 1 cut(s) 778
BsuRI GGCC 5 cut(s) 248, 330, 696, 744, 770
BtgI CCRYGG 1 cut(s) 745
BtsCI GGATG 2 cut(s) 618, 723
BtsIMutI CAGTG 2 cut(s) 208, 1176
Cac8I GCNNGC 3 cut(s) 421, 595, 1131
CfoI GCGC 2 cut(s) 129, 242
Cfr13I GGNCC 2 cut(s) 695, 737
CseI GACGC 1 cut(s) 565
CsiI ACCWGGT 1 cut(s) 167
Csp6I GTAC 9 cut(s) 90, 96, 215, 563, 831, 884, 1010, 1015, 1149
CviAII CATG 5 cut(s) 180, 272, 623, 746, 1083
CviQI GTAC 9 cut(s) 90, 96, 215, 563, 831, 884, 1010, 1015, 1149
DdeI CTNAG 2 cut(s) 6, 845
DpnI GATC 2 cut(s) 81, 851
DpnII GATC 2 cut(s) 79, 849
DraI TTTAAA 1 cut(s) 253
DraIII CACNNNGTG 1 cut(s) 541
DrdI GACNNNNNNGTC 1 cut(s) 683
DseDI GACNNNNNNGTC 1 cut(s) 683
EaeI YGGCCR 2 cut(s) 742, 768
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
EciI GGCGGA 1 cut(s) 397
Eco130I CCWWGG 4 cut(s) 399, 486, 705, 745
Eco24I GRGCYC 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 177
EcoRII CCWGG 5 cut(s) 167, 739, 765, 857, 976
EcoT14I CCWWGG 4 cut(s) 399, 486, 705, 745
EcoT38I GRGCYC 1 cut(s) 407
ErhI CCWWGG 4 cut(s) 399, 486, 705, 745
FaeI CATG 5 cut(s) 183, 275, 626, 749, 1086
FaqI GGGAC 1 cut(s) 689
FatI CATG 5 cut(s) 179, 271, 622, 745, 1082
FauI CCCGC 1 cut(s) 359
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 4 cut(s) 177, 553, 591, 1087
FokI GGATG 2 cut(s) 605, 730
FriOI GRGCYC 1 cut(s) 407
Fsp4HI GCNGC 4 cut(s) 177, 553, 591, 1087
FspBI CTAG 1 cut(s) 444
GlaI GCGC 2 cut(s) 128, 241
GluI GCNGC 4 cut(s) 177, 553, 591, 1087
GsaI CCCAGC 1 cut(s) 873
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 5 cut(s) 248, 330, 696, 744, 770
HapII CCGG 2 cut(s) 699, 1123
HgaI GACGC 1 cut(s) 565
HhaI GCGC 2 cut(s) 129, 242
Hin1I GRCGYC 2 cut(s) 576, 906
Hin1II CATG 5 cut(s) 183, 275, 626, 749, 1086
Hin6I GCGC 2 cut(s) 127, 240
HinP1I GCGC 2 cut(s) 127, 240
HindIII AAGCTT 2 cut(s) 8, 153
HinfI GANTC 4 cut(s) 143, 197, 792, 1135
HpaII CCGG 2 cut(s) 699, 1123
HphI GGTGA 3 cut(s) 89, 269, 382
Hpy166II GTNNAC 1 cut(s) 959
Hpy188I TCNGA 5 cut(s) 84, 196, 285, 383, 846
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 1 cut(s) 959
Hpy99I CGWCG 1 cut(s) 617
HpyAV CCTTC 3 cut(s) 350, 497, 782
HpyCH4III ACNGT 5 cut(s) 89, 956, 974, 1014, 1180
HpyCH4IV ACGT 2 cut(s) 906, 1107
HpyCH4V TGCA 7 cut(s) 176, 264, 423, 647, 1073, 1086, 1183
HpyF10VI GCNNNNNNNGC 6 cut(s) 395, 416, 429, 732, 741, 762
HpyF3I CTNAG 2 cut(s) 6, 845
HpySE526I ACGT 2 cut(s) 906, 1107
Hsp92I GRCGYC 2 cut(s) 576, 906
Hsp92II CATG 5 cut(s) 183, 275, 626, 749, 1086
HspAI GCGC 2 cut(s) 127, 240
KpnI GGTACC 1 cut(s) 834
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 2 cut(s) 79, 849
LmnI GCTCC 4 cut(s) 119, 410, 458, 762
Lsp1109I GCAGC 4 cut(s) 188, 539, 577, 1098
LweI GCATC 2 cut(s) 385, 627
MabI ACCWGGT 1 cut(s) 167
MaeI CTAG 1 cut(s) 444
MaeII ACGT 2 cut(s) 906, 1107
MaeIII GTNAC 5 cut(s) 136, 257, 274, 370, 802
MalI GATC 2 cut(s) 81, 851
MboI GATC 2 cut(s) 79, 849
MboII GAAGA 2 cut(s) 183, 643
MhlI GDGCHC 2 cut(s) 391, 407
MlsI TGGCCA 2 cut(s) 744, 770
MluCI AATT 3 cut(s) 896, 949, 1111
MluNI TGGCCA 2 cut(s) 744, 770
MlyI GAGTC 2 cut(s) 137, 801
MnlI CCTC 9 cut(s) 82, 199, 219, 377, 670, 778, 840, 977, 1022
Mox20I TGGCCA 2 cut(s) 744, 770
MscI TGGCCA 2 cut(s) 744, 770
MseI TTAA 4 cut(s) 252, 428, 825, 918
Msp20I TGGCCA 2 cut(s) 744, 770
MspA1I CMGCKG 1 cut(s) 1089
MspI CCGG 2 cut(s) 699, 1123
MspR9I CCNGG 6 cut(s) 169, 699, 741, 767, 859, 978
MvaI CCWGG 5 cut(s) 169, 741, 767, 859, 978
MwoI GCNNNNNNNGC 6 cut(s) 395, 416, 429, 732, 741, 762
NciI CCSGG 1 cut(s) 699
NcoI CCATGG 1 cut(s) 745
NdeII GATC 2 cut(s) 79, 849
NlaIII CATG 5 cut(s) 183, 275, 626, 749, 1086
NlaIV GGNNCC 7 cut(s) 187, 406, 460, 697, 703, 764, 832
NmuCI GTSAC 3 cut(s) 257, 274, 370
NspI RCATGY 2 cut(s) 183, 275
PciI ACATGT 1 cut(s) 271
PfeI GAWTC 2 cut(s) 197, 1135
PkrI GCNGC 4 cut(s) 178, 554, 592, 1088
PleI GAGTC 2 cut(s) 137, 800
PpsI GAGTC 2 cut(s) 137, 800
PscI ACATGT 1 cut(s) 271
PshAI GACNNNNGTC 1 cut(s) 511
PshBI ATTAAT 1 cut(s) 918
PsiI TTATAA 1 cut(s) 938
Psp6I CCWGG 5 cut(s) 167, 739, 765, 857, 976
PspFI CCCAGC 1 cut(s) 869
PspGI CCWGG 5 cut(s) 167, 739, 765, 857, 976
PspN4I GGNNCC 7 cut(s) 187, 406, 460, 697, 703, 764, 832
PspPI GGNCC 2 cut(s) 695, 737
PsrI GAACNNNNNNTAC 2 cut(s) 876, 908
PstI CTGCAG 1 cut(s) 178
RsaI GTAC 9 cut(s) 91, 97, 216, 564, 832, 885, 1011, 1016, 1150
RsaNI GTAC 9 cut(s) 90, 96, 215, 563, 831, 884, 1010, 1015, 1149
SaqAI TTAA 4 cut(s) 252, 428, 825, 918
SatI GCNGC 4 cut(s) 177, 553, 591, 1087
Sau3AI GATC 2 cut(s) 79, 849
Sau96I GGNCC 2 cut(s) 695, 737
SchI GAGTC 2 cut(s) 137, 801
ScrFI CCNGG 6 cut(s) 169, 699, 741, 767, 859, 978
SduI GDGCHC 2 cut(s) 391, 407
SexAI ACCWGGT 1 cut(s) 167
SfaNI GCATC 2 cut(s) 385, 627
SfcI CTRYAG 1 cut(s) 174
SinI GGWCC 1 cut(s) 737
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 3 cut(s) 896, 949, 1111
SsiI CCGC 4 cut(s) 366, 408, 735, 1089
SspMI CTAG 1 cut(s) 444
StyD4I CCNGG 6 cut(s) 167, 697, 739, 765, 857, 976
StyI CCWWGG 4 cut(s) 399, 486, 705, 745
TaaI ACNGT 5 cut(s) 89, 956, 974, 1014, 1180
TaiI ACGT 2 cut(s) 909, 1110
TaqI TCGA 3 cut(s) 162, 654, 942
TasI AATT 3 cut(s) 896, 949, 1111
TatI WGTACW 5 cut(s) 89, 95, 562, 883, 1009
TfiI GAWTC 2 cut(s) 197, 1135
Tru1I TTAA 4 cut(s) 252, 428, 825, 918
Tru9I TTAA 4 cut(s) 252, 428, 825, 918
TscAI CASTG 2 cut(s) 208, 1183
TseFI GTSAC 3 cut(s) 257, 274, 370
TseI GCWGC 4 cut(s) 176, 552, 590, 1086
Tsp45I GTSAC 3 cut(s) 257, 274, 370
TspDTI ATGAA 1 cut(s) 28
TspRI CASTG 2 cut(s) 208, 1183
VpaK11BI GGWCC 1 cut(s) 737
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 1 cut(s) 1111
XceI RCATGY 2 cut(s) 183, 275
XspI CTAG 1 cut(s) 444
ZraI GACGTC 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.