RchiOBHm_Chr2g0116641

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001539
Physical Location & Seq
Reverse (-)
28964960 .. 28966530
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48967

Sequence Viewer

Length: 978 bp
ATGACTAAGCTTACAAGTTGTTTTCCCACTGTTGTATTCATTTTTCTGCATATTCTATACCAATCATTTGCTTTTGGTGATGAGACGACTACGAGGACTTATGATGTTCTGAAGTTTGGAGCTAAGCGCAATGGTGTAACCAATTCAACCCAAGCTTTTCTTGATGCTTGGTCTGCAGACTGTGGCTCTGCCGCAGATACCACTGAGATATATGTGCCAAAAGCGGAGACTTTGAGTTTTACAAACTCCAAGGACATTAGAGCTGAAGGACTAATGTCACTAAACAGCCAAATGTTTCACATTGTGATAAACGGCCGCCGAGATGTACTCATTCAAAGCGTCAAAGTCATTGCCGCTGGAAATAGCCCAAACACTGATGGCATCCATGTCCAGTTTTCCAGATATGTTGCAATAGCTTCGACACCTCCATCAAAACTGGGGACGATTGTGTCTCTATTGGCCCCGGCACCAAGGAATTGTGGATGGAACGCATATCATGCGGACCTGGCCATGGCATTAGGTAAGTTAAACATAGGGAGTTTGGCCAAAGACTTGGAAGAGGAAGGAATCCAAAATGTAACACTGAAAAATGCGATTTTCAAGGGTACTACAAATGGTCTCAGGATAAAGTCCTGGGCAAGACCCAGCACCGGGTTTGTGCAAGGTGTCCGATCCCTTGATGTTGAAATGATTAATGTCCAAAACCCCATTGTAATTTACCAGAATTACTGCCCACACAACCTCAACTGTCCTGGTCAGGTATCAGATGTCAAAGTTAACGACGTGTTGTACCAGAACATTCGGGGGACATCAGCAACAGCAGTCGCCATAAAATTTGATTTTAGTGCAGCAAATCCATGCAGTGGAGTGAAACTCGACAACGTGCATTTGACTTGCCGGAAGCAAGTGGTACAATCAGATTGTACCAATGTAAATGTAAAGAGTGTTGGCACTGTGCAACCAAGCAGTTGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

325

Amino Acids

35.23

Weight (kDa)

8.68

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 74 - 314 1.4e-41 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 448
AccB1I GGYRCC 1 cut(s) 466
AccB7I CCANNNNNTGG 1 cut(s) 863
AciI CCGC 5 cut(s) 192, 224, 316, 354, 500
AclWI GGATC 1 cut(s) 666
AcoI YGGCCR 3 cut(s) 313, 507, 543
AcsI RAATTY 1 cut(s) 833
AcuI CTGAAG 2 cut(s) 131, 285
AdeI CACNNNGTG 1 cut(s) 304
AfaI GTAC 5 cut(s) 327, 607, 791, 912, 925
AfiI CCNNNNNNNGG 4 cut(s) 511, 650, 651, 863
AflIII ACRYGT 1 cut(s) 783
AgsI TTSAA 4 cut(s) 147, 335, 601, 686
AjiI CACGTC 1 cut(s) 784
AjnI CCWGG 3 cut(s) 504, 632, 751
AluBI AGCT 5 cut(s) 10, 122, 155, 263, 416
AluI AGCT 5 cut(s) 10, 122, 155, 263, 416
Alw26I GTCTC 4 cut(s) 77, 221, 456, 623
AlwI GGATC 1 cut(s) 666
AoxI GGCC 4 cut(s) 313, 459, 507, 543
ApeKI GCWGC 1 cut(s) 848
ApoI RAATTY 1 cut(s) 833
AseI ATTAAT 1 cut(s) 693
AspLEI GCGC 1 cut(s) 129
AspS9I GGNCC 2 cut(s) 460, 502
AsuC2I CCSGG 2 cut(s) 464, 652
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 502
BalI TGGCCA 2 cut(s) 509, 545
BanI GGYRCC 1 cut(s) 466
BbvI GCAGC 1 cut(s) 860
BccI CCATC 3 cut(s) 371, 436, 477
BceAI ACGGC 1 cut(s) 328
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciT130I CCWGG 3 cut(s) 506, 634, 753
BcnI CCSGG 2 cut(s) 464, 652
BcoDI GTCTC 4 cut(s) 77, 221, 456, 623
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 4 cut(s) 192, 316, 354, 849
BlpI GCTNAGC 1 cut(s) 123
BlsI GCNGC 4 cut(s) 193, 317, 355, 850
Bme1390I CCNGG 5 cut(s) 464, 506, 634, 652, 753
Bme18I GGWCC 1 cut(s) 502
BmgBI CACGTC 1 cut(s) 784
BmgT120I GGNCC 2 cut(s) 460, 502
BmiI GGNNCC 2 cut(s) 462, 468
BmrFI CCNGG 5 cut(s) 464, 506, 634, 652, 753
BmrI ACTGGG 1 cut(s) 446
BmsI GCATC 2 cut(s) 154, 390
BmuI ACTGGG 1 cut(s) 446
BoxI GACNNNNGTC 1 cut(s) 274
BplI GAGNNNNNCTC 4 cut(s) 312, 344, 858, 890
Bpu1102I GCTNAGC 1 cut(s) 123
BpuMI CCSGG 2 cut(s) 464, 652
BsaI GGTCTC 1 cut(s) 623
BsaJI CCNNGG 5 cut(s) 249, 462, 470, 510, 633
Bsc4I CCNNNNNNNGG 4 cut(s) 511, 650, 651, 863
Bse1I ACTGG 2 cut(s) 391, 441
Bse3DI GCAATG 2 cut(s) 136, 348
BseBI CCWGG 3 cut(s) 506, 634, 753
BseDI CCNNGG 5 cut(s) 249, 462, 470, 510, 633
BseGI GGATG 2 cut(s) 381, 488
BseLI CCNNNNNNNGG 4 cut(s) 511, 650, 651, 863
BseMI GCAATG 2 cut(s) 136, 348
BseMII CTCAG 2 cut(s) 195, 634
BseNI ACTGG 2 cut(s) 391, 441
BseX3I CGGCCG 1 cut(s) 313
BseXI GCAGC 1 cut(s) 860
BseYI CCCAGC 1 cut(s) 644
BsgI GTGCAG 1 cut(s) 867
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 4 cut(s) 315, 461, 509, 545
BshNI GGYRCC 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 316
BsiSI CCGG 3 cut(s) 464, 651, 898
BslFI GGGAC 2 cut(s) 454, 820
BslI CCNNNNNNNGG 4 cut(s) 511, 650, 651, 863
BsmAI GTCTC 4 cut(s) 77, 221, 456, 623
BsmBI CGTCTC 1 cut(s) 77
BsmFI GGGAC 2 cut(s) 454, 820
BsnI GGCC 4 cut(s) 315, 461, 509, 545
Bso31I GGTCTC 1 cut(s) 623
Bsp143I GATC 1 cut(s) 671
Bsp1720I GCTNAGC 1 cut(s) 123
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 5 cut(s) 192, 224, 316, 354, 500
BspANI GGCC 4 cut(s) 315, 461, 509, 545
BspCNI CTCAG 2 cut(s) 196, 633
BspLI GGNNCC 2 cut(s) 462, 468
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 466
BspTNI GGTCTC 1 cut(s) 623
BsrDI GCAATG 2 cut(s) 136, 348
BsrI ACTGG 2 cut(s) 391, 441
BssECI CCNNGG 5 cut(s) 249, 462, 470, 510, 633
BssMI GATC 1 cut(s) 671
BssT1I CCWWGG 3 cut(s) 249, 470, 510
Bst2UI CCWGG 3 cut(s) 506, 634, 753
Bst4CI ACNGT 4 cut(s) 31, 182, 749, 955
Bst6I CTCTTC 1 cut(s) 552
BstAPI GCANNNNNTGC 1 cut(s) 497
BstDEI CTNAG 4 cut(s) 6, 123, 204, 620
BstDSI CCRYGG 1 cut(s) 510
BstF5I GGATG 2 cut(s) 381, 488
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 1 cut(s) 674
BstMAI GTCTC 4 cut(s) 77, 221, 456, 623
BstMBI GATC 1 cut(s) 671
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 3 cut(s) 173, 497, 506
BstNI CCWGG 3 cut(s) 506, 634, 753
BstPAI GACNNNNGTC 1 cut(s) 274
BstSCI CCNGG 5 cut(s) 462, 504, 632, 650, 751
BstSFI CTRYAG 1 cut(s) 174
BstV1I GCAGC 1 cut(s) 860
BstXI CCANNNNNNTGG 1 cut(s) 553
BstZI CGGCCG 1 cut(s) 313
BsuRI GGCC 4 cut(s) 315, 461, 509, 545
BtgI CCRYGG 1 cut(s) 510
BtrI CACGTC 1 cut(s) 784
BtsCI GGATG 2 cut(s) 381, 488
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 6 cut(s) 27, 201, 372, 581, 868, 951
CfoI GCGC 1 cut(s) 129
Cfr13I GGNCC 2 cut(s) 460, 502
CseI GACGC 1 cut(s) 328
Csp6I GTAC 5 cut(s) 326, 606, 790, 911, 924
CviAII CATG 4 cut(s) 386, 497, 511, 858
CviQI GTAC 5 cut(s) 326, 606, 790, 911, 924
DdeI CTNAG 4 cut(s) 6, 123, 204, 620
DpnI GATC 1 cut(s) 673
DpnII GATC 1 cut(s) 671
DraIII CACNNNGTG 1 cut(s) 304
DrdI GACNNNNNNGTC 1 cut(s) 448
DseDI GACNNNNNNGTC 1 cut(s) 448
EaeI YGGCCR 3 cut(s) 313, 507, 543
EagI CGGCCG 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EclXI CGGCCG 1 cut(s) 313
Eco130I CCWWGG 3 cut(s) 249, 470, 510
Eco31I GGTCTC 1 cut(s) 623
Eco47I GGWCC 1 cut(s) 502
Eco52I CGGCCG 1 cut(s) 313
Eco57I CTGAAG 2 cut(s) 131, 285
EcoRII CCWGG 3 cut(s) 504, 632, 751
EcoT14I CCWWGG 3 cut(s) 249, 470, 510
ErhI CCWWGG 3 cut(s) 249, 470, 510
Esp3I CGTCTC 1 cut(s) 77
FaeI CATG 4 cut(s) 389, 500, 514, 861
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FaqI GGGAC 2 cut(s) 454, 820
FatI CATG 4 cut(s) 385, 496, 510, 857
Fnu4HI GCNGC 4 cut(s) 192, 316, 354, 849
FokI GGATG 2 cut(s) 368, 495
Fsp4HI GCNGC 4 cut(s) 192, 316, 354, 849
GlaI GCGC 1 cut(s) 128
GluI GCNGC 4 cut(s) 192, 316, 354, 849
GsaI CCCAGC 1 cut(s) 648
HaeIII GGCC 4 cut(s) 315, 461, 509, 545
HapII CCGG 3 cut(s) 464, 651, 898
HgaI GACGC 1 cut(s) 328
HhaI GCGC 1 cut(s) 129
Hin1II CATG 4 cut(s) 389, 500, 514, 861
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HindIII AAGCTT 2 cut(s) 8, 153
HinfI GANTC 1 cut(s) 567
HpaI GTTAAC 1 cut(s) 778
HpaII CCGG 3 cut(s) 464, 651, 898
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 778
Hpy188I TCNGA 4 cut(s) 111, 671, 766, 919
Hpy188III TCNNGA 3 cut(s) 161, 399, 622
Hpy8I GTNNAC 1 cut(s) 778
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 2 cut(s) 260, 557
HpyCH4III ACNGT 4 cut(s) 31, 182, 749, 955
HpyCH4IV ACGT 2 cut(s) 783, 882
HpyCH4V TGCA 8 cut(s) 49, 176, 410, 661, 848, 861, 886, 958
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 497, 506
HpyF3I CTNAG 4 cut(s) 6, 123, 204, 620
HpySE526I ACGT 2 cut(s) 783, 882
Hsp92II CATG 4 cut(s) 389, 500, 514, 861
HspAI GCGC 1 cut(s) 127
KspAI GTTAAC 1 cut(s) 778
Kzo9I GATC 1 cut(s) 671
LmnI GCTCC 1 cut(s) 119
Lsp1109I GCAGC 1 cut(s) 860
LweI GCATC 2 cut(s) 154, 390
MaeII ACGT 2 cut(s) 783, 882
MaeIII GTNAC 3 cut(s) 136, 276, 577
MalI GATC 1 cut(s) 673
MboI GATC 1 cut(s) 671
MboII GAAGA 1 cut(s) 569
MlsI TGGCCA 2 cut(s) 509, 545
MluCI AATT 5 cut(s) 142, 475, 714, 724, 833
MluNI TGGCCA 2 cut(s) 509, 545
MnlI CCTC 4 cut(s) 87, 435, 553, 752
Mox20I TGGCCA 2 cut(s) 509, 545
MscI TGGCCA 2 cut(s) 509, 545
MseI TTAA 3 cut(s) 527, 693, 777
Msp20I TGGCCA 2 cut(s) 509, 545
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 3 cut(s) 464, 651, 898
MspR9I CCNGG 5 cut(s) 464, 506, 634, 652, 753
MvaI CCWGG 3 cut(s) 506, 634, 753
MwoI GCNNNNNNNGC 3 cut(s) 173, 497, 506
NciI CCSGG 2 cut(s) 464, 652
NcoI CCATGG 1 cut(s) 510
NdeII GATC 1 cut(s) 671
NlaIII CATG 4 cut(s) 389, 500, 514, 861
NlaIV GGNNCC 2 cut(s) 462, 468
NmeAIII GCCGAG 1 cut(s) 344
NmuCI GTSAC 1 cut(s) 276
PfeI GAWTC 1 cut(s) 567
PflMI CCANNNNNTGG 1 cut(s) 863
PkrI GCNGC 4 cut(s) 193, 317, 355, 850
PshAI GACNNNNGTC 1 cut(s) 274
PshBI ATTAAT 1 cut(s) 693
Psp6I CCWGG 3 cut(s) 504, 632, 751
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 3 cut(s) 504, 632, 751
PspN4I GGNNCC 2 cut(s) 462, 468
PspPI GGNCC 2 cut(s) 460, 502
PstI CTGCAG 1 cut(s) 178
RsaI GTAC 5 cut(s) 327, 607, 791, 912, 925
RsaNI GTAC 5 cut(s) 326, 606, 790, 911, 924
SaqAI TTAA 3 cut(s) 527, 693, 777
SatI GCNGC 4 cut(s) 192, 316, 354, 849
Sau3AI GATC 1 cut(s) 671
Sau96I GGNCC 2 cut(s) 460, 502
ScrFI CCNGG 5 cut(s) 464, 506, 634, 652, 753
SfaNI GCATC 2 cut(s) 154, 390
SfcI CTRYAG 1 cut(s) 174
SinI GGWCC 1 cut(s) 502
Sse9I AATT 5 cut(s) 142, 475, 714, 724, 833
SsiI CCGC 5 cut(s) 192, 224, 316, 354, 500
StyD4I CCNGG 5 cut(s) 462, 504, 632, 650, 751
StyI CCWWGG 3 cut(s) 249, 470, 510
TaaI ACNGT 4 cut(s) 31, 182, 749, 955
TaiI ACGT 2 cut(s) 786, 885
TaqI TCGA 2 cut(s) 419, 876
TasI AATT 5 cut(s) 142, 475, 714, 724, 833
TatI WGTACW 1 cut(s) 325
TauI GCSGC 3 cut(s) 194, 318, 356
TfiI GAWTC 1 cut(s) 567
Tru1I TTAA 3 cut(s) 527, 693, 777
Tru9I TTAA 3 cut(s) 527, 693, 777
TscAI CASTG 6 cut(s) 34, 208, 379, 588, 868, 958
TseFI GTSAC 1 cut(s) 276
TseI GCWGC 1 cut(s) 848
Tsp45I GTSAC 1 cut(s) 276
TspDTI ATGAA 1 cut(s) 28
TspRI CASTG 6 cut(s) 34, 208, 379, 588, 868, 958
Van91I CCANNNNNTGG 1 cut(s) 863
VpaK11BI GGWCC 1 cut(s) 502
VspI ATTAAT 1 cut(s) 693
XapI RAATTY 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.