Rw2G020860

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001539
Physical Location & Seq
Reverse (-)
28584217 .. 28590271
6055 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020860.1

Sequence Viewer

Length: 1137 bp
ATGATTCAGCTTACAAGGTGTTTTGCCACGGTTGTATTCCTTTTTCTTACAATAATACACCAATCGTCTGCTGCTGACGAGACTACAATAACTTCTTACAATGTCCTGAGCTTTGGAGCTAAGCGTAATGGTGTAACGGATTCCACCCAAGCTTTTCTTGATGCTTGGGCTGCTGCCTCCTTTAAAGGTGACTGCAAAAGCTCACATGTCACTTTTCGGATTGATGGAACACTCGTGGCTTCGCCTGATTACCGAGTACTAGGCCAAGCTAATAACTGGTTCAGTTTTGAAGGAGTTACTGGGGTAAACATTGTTGGAGGAGCACTTGATGCTAAGGGTTCCGCCTTGTGGGCTTGCAAATTAAGTACCGAAACCACCAGCTGTCCTAATGGAGCCACGACTTTGAGTTTTACAAACTCAAAAGACATTAGAGTTGAAGGACTAATGTCATTGAACAGCCAAATGTTTCACATTGTGATAAACGGCTGCCAGGATGTACTCATTCGAGGCGTCAAAATTATTGCTGCTGGCAATAGCCCTAACACCGACGGCATTCATGTCCAATTTTCCAAAAATGTTGCAATCTTCGACACCTCCTTGAAAACTGGGGACGACTGTGTCTCCATTGGCCCTGGGACCAAGGACTTGTGGATGGAACGCATATCATGCGGACCTGGCCACGGCATTAGTATAGGGAGCCTAGCCAAAGACTTGGAAGAGGAAGGAGTACAAAACGTAACAGTTAAAAATGCAACTTTTAAGGGTACCACAAATGGGCTGAGGATCAAATCTTGGGCGAGACCTAGCACCGGGTTTGTCCAAGGTGTTAGATTCCTTGATGTCGTAATGCTTAATGTCCAAAATCCCATCGTAATTGACCAGAATTACTGCCCACACAACCTCAACTGTCCTGGTAAGGTATCTGGTGTCAAAGTCAGCGATGTACTGTACCAGAACATTCGAGGCACATCGACAACAGCAGTTGCAGTAAAATTTGATTGCAGTGCAACAAATCCATGCAGTGGGATAAGACTCGAAAATGTGAATTTGACTTGCCGGAAGCAAGTAGTTCAATCATACTGTGCAAATGTAAATGGAAAGAGTGTTGGCACTATTCAACCAAGCAGTTGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

378

Amino Acids

40.36

Weight (kDa)

8.35

Isoelectric Point (pI)

22.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 59 - 365 3.5e-83 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
Acc65I GGTACC 1 cut(s) 764
AccB1I GGYRCC 1 cut(s) 764
AccB7I CCANNNNNTGG 1 cut(s) 1022
AciI CCGC 2 cut(s) 342, 669
AclWI GGATC 1 cut(s) 791
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 2 cut(s) 992, 1045
AcyI GRCGYC 1 cut(s) 510
AdeI CACNNNGTG 1 cut(s) 475
AfaI GTAC 7 cut(s) 258, 367, 498, 729, 766, 945, 950
AfiI CCNNNNNNNGG 5 cut(s) 348, 680, 774, 809, 1022
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 6 cut(s) 290, 437, 454, 601, 1073, 1118
AjnI CCWGG 4 cut(s) 489, 631, 673, 910
AluBI AGCT 7 cut(s) 10, 111, 119, 152, 201, 269, 381
AluI AGCT 7 cut(s) 10, 111, 119, 152, 201, 269, 381
Alw21I GWGCWC 1 cut(s) 325
Alw26I GTCTC 3 cut(s) 74, 625, 793
AlwI GGATC 1 cut(s) 791
AoxI GGCC 3 cut(s) 262, 628, 676
ApeKI GCWGC 5 cut(s) 71, 170, 173, 486, 524
ApoI RAATTY 2 cut(s) 992, 1045
Asp718I GGTACC 1 cut(s) 764
AspS9I GGNCC 3 cut(s) 629, 636, 671
AsuC2I CCSGG 1 cut(s) 811
AsuHPI GGTGA 1 cut(s) 200
AvaII GGWCC 2 cut(s) 636, 671
BalI TGGCCA 1 cut(s) 678
BanI GGYRCC 1 cut(s) 764
BauI CACGAG 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 325
BbvCI CCTCAGC 1 cut(s) 779
BbvI GCAGC 5 cut(s) 58, 157, 160, 473, 511
BccI CCATC 3 cut(s) 218, 646, 875
BceAI ACGGC 3 cut(s) 499, 565, 697
BciT130I CCWGG 4 cut(s) 491, 633, 675, 912
BcnI CCSGG 1 cut(s) 811
BcoDI GTCTC 3 cut(s) 74, 625, 793
BfaI CTAG 3 cut(s) 260, 701, 804
BglI GCCNNNNNGGC 1 cut(s) 350
BisI GCNGC 5 cut(s) 72, 171, 174, 487, 525
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 5 cut(s) 73, 172, 175, 488, 526
BmcAI AGTACT 1 cut(s) 258
Bme1390I CCNGG 5 cut(s) 491, 633, 675, 811, 912
Bme18I GGWCC 2 cut(s) 636, 671
BmgT120I GGNCC 3 cut(s) 629, 636, 671
BmiI GGNNCC 5 cut(s) 340, 394, 637, 698, 766
BmrFI CCNGG 5 cut(s) 491, 633, 675, 811, 912
BmrI ACTGGG 2 cut(s) 309, 615
BmsI GCATC 2 cut(s) 151, 319
BmuI ACTGGG 2 cut(s) 309, 615
BoxI GACNNNNGTC 1 cut(s) 445
Bpu10I CCTNAGC 3 cut(s) 107, 333, 779
Bpu1102I GCTNAGC 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 811
BsaHI GRCGYC 1 cut(s) 510
BsaI GGTCTC 1 cut(s) 793
BsaJI CCNNGG 6 cut(s) 27, 631, 632, 639, 679, 820
BsaXI ACNNNNNCTCC 2 cut(s) 605, 635
Bsc4I CCNNNNNNNGG 5 cut(s) 348, 680, 774, 809, 1022
Bse1I ACTGG 3 cut(s) 281, 304, 610
BseBI CCWGG 4 cut(s) 491, 633, 675, 912
BseDI CCNNGG 6 cut(s) 27, 631, 632, 639, 679, 820
BseGI GGATG 2 cut(s) 499, 657
BseLI CCNNNNNNNGG 5 cut(s) 348, 680, 774, 809, 1022
BseMII CTCAG 2 cut(s) 98, 770
BseNI ACTGG 3 cut(s) 281, 304, 610
BseRI GAGGAG 1 cut(s) 333
BseXI GCAGC 5 cut(s) 58, 157, 160, 473, 511
BshFI GGCC 3 cut(s) 264, 630, 678
BshNI GGYRCC 1 cut(s) 764
BsiHKAI GWGCWC 1 cut(s) 325
BsiSI CCGG 2 cut(s) 810, 1057
BslFI GGGAC 2 cut(s) 623, 649
BslI CCNNNNNNNGG 5 cut(s) 348, 680, 774, 809, 1022
BsmAI GTCTC 3 cut(s) 74, 625, 793
BsmFI GGGAC 2 cut(s) 623, 649
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 3 cut(s) 264, 630, 678
Bso31I GGTCTC 1 cut(s) 793
Bsp1286I GDGCHC 1 cut(s) 325
Bsp143I GATC 1 cut(s) 783
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 2 cut(s) 342, 669
BspANI GGCC 3 cut(s) 264, 630, 678
BspCNI CTCAG 2 cut(s) 99, 771
BspLI GGNNCC 5 cut(s) 340, 394, 637, 698, 766
BspPI GGATC 1 cut(s) 791
BspT107I GGYRCC 1 cut(s) 764
BspTNI GGTCTC 1 cut(s) 793
BsrI ACTGG 3 cut(s) 281, 304, 610
BssECI CCNNGG 6 cut(s) 27, 631, 632, 639, 679, 820
BssMI GATC 1 cut(s) 783
BssNI GRCGYC 1 cut(s) 510
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 639, 820
Bst2BI CACGAG 1 cut(s) 233
Bst2UI CCWGG 4 cut(s) 491, 633, 675, 912
Bst4CI ACNGT 6 cut(s) 31, 617, 742, 908, 948, 1082
Bst6I CTCTTC 1 cut(s) 711
BstACI GRCGYC 1 cut(s) 510
BstAPI GCANNNNNTGC 2 cut(s) 329, 666
BstC8I GCNNGC 2 cut(s) 355, 529
BstDEI CTNAG 4 cut(s) 107, 120, 333, 779
BstDSI CCRYGG 2 cut(s) 27, 679
BstF5I GGATG 2 cut(s) 499, 657
BstKTI GATC 1 cut(s) 786
BstMAI GTCTC 3 cut(s) 74, 625, 793
BstMBI GATC 1 cut(s) 783
BstMWI GCNNNNNNNGC 5 cut(s) 170, 329, 350, 666, 675
BstNI CCWGG 4 cut(s) 491, 633, 675, 912
BstNSI RCATGY 1 cut(s) 209
BstPAI GACNNNNGTC 1 cut(s) 445
BstSCI CCNGG 5 cut(s) 489, 631, 673, 809, 910
BstV1I GCAGC 5 cut(s) 58, 157, 160, 473, 511
BstXI CCANNNNNNTGG 1 cut(s) 712
BsuRI GGCC 3 cut(s) 264, 630, 678
BtgI CCRYGG 2 cut(s) 27, 679
BtgZI GCGATG 1 cut(s) 954
BtsCI GGATG 2 cut(s) 499, 657
BtsI GCAGTG 2 cut(s) 1009, 1027
BtsIMutI CAGTG 2 cut(s) 1009, 1027
Cac8I GCNNGC 2 cut(s) 355, 529
Cfr13I GGNCC 3 cut(s) 629, 636, 671
CseI GACGC 1 cut(s) 499
Csp6I GTAC 7 cut(s) 257, 366, 497, 728, 765, 944, 949
CviAII CATG 4 cut(s) 206, 557, 666, 1017
CviQI GTAC 7 cut(s) 257, 366, 497, 728, 765, 944, 949
DdeI CTNAG 4 cut(s) 107, 120, 333, 779
DpnI GATC 1 cut(s) 785
DpnII GATC 1 cut(s) 783
DraI TTTAAA 1 cut(s) 184
DraIII CACNNNGTG 1 cut(s) 475
DrdI GACNNNNNNGTC 1 cut(s) 617
DseDI GACNNNNNNGTC 1 cut(s) 617
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
EciI GGCGGA 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 639, 820
Eco31I GGTCTC 1 cut(s) 793
Eco47I GGWCC 2 cut(s) 636, 671
EcoRII CCWGG 4 cut(s) 489, 631, 673, 910
EcoT14I CCWWGG 2 cut(s) 639, 820
ErhI CCWWGG 2 cut(s) 639, 820
FaeI CATG 4 cut(s) 209, 560, 669, 1020
FaiI YATR 7 cut(s) 207, 558, 662, 667, 692, 1018, 1078
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FaqI GGGAC 2 cut(s) 623, 649
FatI CATG 4 cut(s) 205, 556, 665, 1016
Fnu4HI GCNGC 5 cut(s) 72, 171, 174, 487, 525
FokI GGATG 2 cut(s) 506, 664
Fsp4HI GCNGC 5 cut(s) 72, 171, 174, 487, 525
FspBI CTAG 3 cut(s) 260, 701, 804
GluI GCNGC 5 cut(s) 72, 171, 174, 487, 525
HaeIII GGCC 3 cut(s) 264, 630, 678
HapII CCGG 2 cut(s) 810, 1057
HgaI GACGC 1 cut(s) 499
Hin1I GRCGYC 1 cut(s) 510
Hin1II CATG 4 cut(s) 209, 560, 669, 1020
HindIII AAGCTT 1 cut(s) 150
HinfI GANTC 4 cut(s) 4, 140, 831, 1032
HpaII CCGG 2 cut(s) 810, 1057
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 1 cut(s) 219
Hpy188III TCNNGA 2 cut(s) 106, 158
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 3 cut(s) 284, 431, 716
HpyCH4III ACNGT 6 cut(s) 31, 617, 742, 908, 948, 1082
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 9 cut(s) 195, 357, 581, 752, 986, 1002, 1007, 1020, 1085
HpyF10VI GCNNNNNNNGC 5 cut(s) 170, 329, 350, 666, 675
HpyF3I CTNAG 4 cut(s) 107, 120, 333, 779
HpySE526I ACGT 1 cut(s) 735
Hsp92I GRCGYC 1 cut(s) 510
Hsp92II CATG 4 cut(s) 209, 560, 669, 1020
KpnI GGTACC 1 cut(s) 768
Kzo9I GATC 1 cut(s) 783
LmnI GCTCC 4 cut(s) 116, 320, 392, 696
Lsp1109I GCAGC 5 cut(s) 58, 157, 160, 473, 511
LweI GCATC 2 cut(s) 151, 319
MaeI CTAG 3 cut(s) 260, 701, 804
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 5 cut(s) 133, 188, 208, 295, 736
MalI GATC 1 cut(s) 785
MboI GATC 1 cut(s) 783
MboII GAAGA 2 cut(s) 577, 728
MhlI GDGCHC 1 cut(s) 325
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 7 cut(s) 359, 516, 563, 873, 883, 992, 1045
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 1 cut(s) 1026
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 8 cut(s) 187, 311, 500, 604, 712, 774, 911, 956
Mox20I TGGCCA 1 cut(s) 678
MscI TGGCCA 1 cut(s) 678
MseI TTAA 5 cut(s) 183, 362, 744, 759, 852
Msp20I TGGCCA 1 cut(s) 678
MspA1I CMGCKG 1 cut(s) 381
MspI CCGG 2 cut(s) 810, 1057
MspR9I CCNGG 5 cut(s) 491, 633, 675, 811, 912
Mva1269I GAATGC 1 cut(s) 552
MvaI CCWGG 4 cut(s) 491, 633, 675, 912
MwoI GCNNNNNNNGC 5 cut(s) 170, 329, 350, 666, 675
NciI CCSGG 1 cut(s) 811
NdeII GATC 1 cut(s) 783
NlaIII CATG 4 cut(s) 209, 560, 669, 1020
NlaIV GGNNCC 5 cut(s) 340, 394, 637, 698, 766
NmuCI GTSAC 2 cut(s) 188, 208
NspI RCATGY 1 cut(s) 209
PasI CCCWGGG 1 cut(s) 632
PciI ACATGT 1 cut(s) 205
PctI GAATGC 1 cut(s) 552
PfeI GAWTC 3 cut(s) 4, 140, 831
PflFI GACNNNGTC 1 cut(s) 617
PflMI CCANNNNNTGG 1 cut(s) 1022
PkrI GCNGC 5 cut(s) 73, 172, 175, 488, 526
PleI GAGTC 1 cut(s) 1026
PpsI GAGTC 1 cut(s) 1026
PscI ACATGT 1 cut(s) 205
PshAI GACNNNNGTC 1 cut(s) 445
Psp6I CCWGG 4 cut(s) 489, 631, 673, 910
PspGI CCWGG 4 cut(s) 489, 631, 673, 910
PspN4I GGNNCC 5 cut(s) 340, 394, 637, 698, 766
PspPI GGNCC 3 cut(s) 629, 636, 671
PsyI GACNNNGTC 1 cut(s) 617
PvuII CAGCTG 1 cut(s) 381
RsaI GTAC 7 cut(s) 258, 367, 498, 729, 766, 945, 950
RsaNI GTAC 7 cut(s) 257, 366, 497, 728, 765, 944, 949
SaqAI TTAA 5 cut(s) 183, 362, 744, 759, 852
SatI GCNGC 5 cut(s) 72, 171, 174, 487, 525
Sau3AI GATC 1 cut(s) 783
Sau96I GGNCC 3 cut(s) 629, 636, 671
ScaI AGTACT 1 cut(s) 258
SchI GAGTC 1 cut(s) 1026
ScrFI CCNGG 5 cut(s) 491, 633, 675, 811, 912
SduI GDGCHC 1 cut(s) 325
SfaNI GCATC 2 cut(s) 151, 319
SinI GGWCC 2 cut(s) 636, 671
Sse9I AATT 7 cut(s) 359, 516, 563, 873, 883, 992, 1045
SsiI CCGC 2 cut(s) 342, 669
SspMI CTAG 3 cut(s) 260, 701, 804
StyD4I CCNGG 5 cut(s) 489, 631, 673, 809, 910
StyI CCWWGG 2 cut(s) 639, 820
TaaI ACNGT 6 cut(s) 31, 617, 742, 908, 948, 1082
TaiI ACGT 1 cut(s) 738
TaqI TCGA 5 cut(s) 505, 588, 961, 971, 1035
TasI AATT 7 cut(s) 359, 516, 563, 873, 883, 992, 1045
TatI WGTACW 4 cut(s) 256, 496, 727, 943
TfiI GAWTC 3 cut(s) 4, 140, 831
Tru1I TTAA 5 cut(s) 183, 362, 744, 759, 852
Tru9I TTAA 5 cut(s) 183, 362, 744, 759, 852
TscAI CASTG 2 cut(s) 1009, 1027
TseFI GTSAC 2 cut(s) 188, 208
TseI GCWGC 5 cut(s) 71, 170, 173, 486, 524
Tsp45I GTSAC 2 cut(s) 188, 208
TspDTI ATGAA 1 cut(s) 545
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 2 cut(s) 1009, 1027
Tth111I GACNNNGTC 1 cut(s) 617
Van91I CCANNNNNTGG 1 cut(s) 1022
VpaK11BI GGWCC 2 cut(s) 636, 671
XapI RAATTY 2 cut(s) 992, 1045
XceI RCATGY 1 cut(s) 209
XspI CTAG 3 cut(s) 260, 701, 804
ZrmI AGTACT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.