RLG00000018307

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001539
Physical Location & Seq
Reverse (-)
28168742 .. 28170250
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018307

Sequence Viewer

Length: 1212 bp
ATGCTAAGCAAAATGATTCAGCTTACAAGGTGTTTTACCACAGTTGTATTCCTTATTCTTACAATAATACACCAATCGTCTGCTGCTGATCAGACTACAATCACTTACAATGTCCTGAGCTTTGGAGCTAAGCGTAACGGTGTAACGGATTCCACCCAAGCTTTTCTTGATGCATGGAGTGCTGTATGTGAAGCCTCCACGGATTCCTCTGAGATATATGTACCGAAGGGGAGGTATTTGCTTGGCGCTATGGCCTTTAAAGGTGACTGCAAAAGCTCACATATGACTTTTCGGATTGATGGAACACTCGTGGCTTCACCTGATTACCGAGTACTAGGCCAAGCTAACAACTGGTTCAGTTTTCAAGGAGTTACTGGGGTAAACATTGTCGGAGGGGCGCTTGATGCTAAGGGTTCCGCCTTGTGGGCTTGCAAATTAAGTACCGAAACCACCAGCTGTCCTAATGGATCCACGACTTTGAGTTTTACAAACTCAAAAGACATTAGAGTTGAAGGACTAATGTCATTGAACAGCCAAATGTTTCACAATGTGATAAATGGCTGCCAGGATGTACTCATTCAAGGCGTCAAAATTATTGCTGCTGGCAAAAGCCCTAACACCGACGGCATTCATGTCCAATTTTCTAAAAATGTTGCAATCTTCGACACCTCCATCAAAACTGGGGACGACTGTGTCTCTATTGGCCCTGGGACCAAGGACTTGTGGATGGAACGCATATCATGCGGACCTGGCCACGGCATTAGCATAGGGAGCCTGGCCAAAGACTTAGAAGAGGAAGGAGTACAAAACGTAACAGTTAAAAATGCAATTTTTAAGGGTACAACAAATGGGCTGAGGATCAAATCTTGGGCAAGACCTAGCACCGGGTTTGTCCAAGGTGTTCGATTCCTTGATGTCGTAATGCTTAATGTCCAAAATCCCATCGTAATTGACCAGAATTACTGCCCACACAACCTCAACTGTCCTGGTCAGGTATCTGGTGTCAAAGTTCGCGATGTACTGTACCAGAACATTCGAGGCACATCGACAACAGCAGTTGCAGTAAAATTTGATTGCAGTGCAACAAATCCATGCAGTGGGATAAGACTCGAAAATGTGAATTTGACTTGCCGGAAGCAAGTAGTTCAATCATACTGTATGAATGTAAATGGAAAGAGTGTTGGCACTATTCAACCAAGCAGTTGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

404

Amino Acids

43.3

Weight (kDa)

8.57

Isoelectric Point (pI)

26.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 37 - 81 6.9e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 67 - 390 1.1e-85 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 692
AccB7I CCANNNNNTGG 1 cut(s) 1097
AccII CGCG 1 cut(s) 1014
AciI CCGC 2 cut(s) 417, 744
AclWI GGATC 3 cut(s) 462, 475, 866
AcoI YGGCCR 2 cut(s) 751, 777
AcsI RAATTY 2 cut(s) 1067, 1120
AcyI GRCGYC 1 cut(s) 585
AdeI CACNNNGTG 1 cut(s) 550
AfaI GTAC 8 cut(s) 222, 333, 442, 573, 804, 841, 1020, 1025
AfiI CCNNNNNNNGG 4 cut(s) 423, 755, 884, 1097
AgsI TTSAA 6 cut(s) 365, 512, 529, 581, 1148, 1193
AjnI CCWGG 5 cut(s) 564, 706, 748, 774, 985
AluBI AGCT 7 cut(s) 22, 120, 128, 161, 276, 344, 456
AluI AGCT 7 cut(s) 22, 120, 128, 161, 276, 344, 456
Alw26I GTCTC 1 cut(s) 700
AlwI GGATC 3 cut(s) 462, 475, 866
AoxI GGCC 5 cut(s) 252, 337, 703, 751, 777
ApeKI GCWGC 3 cut(s) 83, 561, 599
ApoI RAATTY 2 cut(s) 1067, 1120
AspLEI GCGC 2 cut(s) 248, 400
AspS9I GGNCC 3 cut(s) 704, 711, 746
AsuC2I CCSGG 1 cut(s) 886
AsuHPI GGTGA 2 cut(s) 275, 309
AvaII GGWCC 2 cut(s) 711, 746
BalI TGGCCA 2 cut(s) 753, 779
BamHI GGATCC 1 cut(s) 467
BauI CACGAG 1 cut(s) 308
BbvCI CCTCAGC 1 cut(s) 854
BbvI GCAGC 3 cut(s) 70, 548, 586
BccI CCATC 4 cut(s) 293, 680, 721, 950
BceAI ACGGC 2 cut(s) 640, 772
BciT130I CCWGG 5 cut(s) 566, 708, 750, 776, 987
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 1 cut(s) 886
BcoDI GTCTC 1 cut(s) 700
BfaI CTAG 2 cut(s) 335, 879
BfoI RGCGCY 2 cut(s) 249, 401
BglI GCCNNNNNGGC 1 cut(s) 425
BisI GCNGC 3 cut(s) 84, 562, 600
BlpI GCTNAGC 2 cut(s) 5, 129
BlsI GCNGC 3 cut(s) 85, 563, 601
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 6 cut(s) 566, 708, 750, 776, 886, 987
Bme18I GGWCC 2 cut(s) 711, 746
BmgT120I GGNCC 3 cut(s) 704, 711, 746
BmiI GGNNCC 4 cut(s) 415, 469, 712, 773
BmrFI CCNGG 6 cut(s) 566, 708, 750, 776, 886, 987
BmrI ACTGGG 2 cut(s) 384, 690
BmsI GCATC 2 cut(s) 160, 394
BmuI ACTGGG 2 cut(s) 384, 690
BoxI GACNNNNGTC 1 cut(s) 520
Bpu10I CCTNAGC 3 cut(s) 116, 408, 854
Bpu1102I GCTNAGC 2 cut(s) 5, 129
BpuMI CCSGG 1 cut(s) 886
BsaHI GRCGYC 1 cut(s) 585
BsaJI CCNNGG 6 cut(s) 198, 706, 707, 714, 754, 895
Bsc4I CCNNNNNNNGG 4 cut(s) 423, 755, 884, 1097
Bse1I ACTGG 3 cut(s) 356, 379, 685
BseBI CCWGG 5 cut(s) 566, 708, 750, 776, 987
BseDI CCNNGG 6 cut(s) 198, 706, 707, 714, 754, 895
BseGI GGATG 2 cut(s) 574, 732
BseLI CCNNNNNNNGG 4 cut(s) 423, 755, 884, 1097
BseMII CTCAG 3 cut(s) 107, 201, 845
BseNI ACTGG 3 cut(s) 356, 379, 685
BseXI GCAGC 3 cut(s) 70, 548, 586
Bsh1236I CGCG 1 cut(s) 1014
BshFI GGCC 5 cut(s) 254, 339, 705, 753, 779
BsiSI CCGG 2 cut(s) 885, 1132
BslFI GGGAC 2 cut(s) 698, 724
BslI CCNNNNNNNGG 4 cut(s) 423, 755, 884, 1097
BsmAI GTCTC 1 cut(s) 700
BsmFI GGGAC 2 cut(s) 698, 724
BsmI GAATGC 1 cut(s) 627
BsnI GGCC 5 cut(s) 254, 339, 705, 753, 779
Bsp143I GATC 3 cut(s) 88, 467, 858
Bsp1720I GCTNAGC 2 cut(s) 5, 129
Bsp68I TCGCGA 1 cut(s) 1014
BspACI CCGC 2 cut(s) 417, 744
BspANI GGCC 5 cut(s) 254, 339, 705, 753, 779
BspCNI CTCAG 3 cut(s) 108, 202, 846
BspFNI CGCG 1 cut(s) 1014
BspLI GGNNCC 4 cut(s) 415, 469, 712, 773
BspPI GGATC 3 cut(s) 462, 475, 866
BsrI ACTGG 3 cut(s) 356, 379, 685
BssECI CCNNGG 6 cut(s) 198, 706, 707, 714, 754, 895
BssMI GATC 3 cut(s) 88, 467, 858
BssNI GRCGYC 1 cut(s) 585
BssSI CACGAG 1 cut(s) 308
BssT1I CCWWGG 2 cut(s) 714, 895
Bst2BI CACGAG 1 cut(s) 308
Bst2UI CCWGG 5 cut(s) 566, 708, 750, 776, 987
Bst4CI ACNGT 7 cut(s) 43, 140, 692, 817, 983, 1023, 1157
Bst6I CTCTTC 1 cut(s) 786
BstACI GRCGYC 1 cut(s) 585
BstAPI GCANNNNNTGC 2 cut(s) 179, 741
BstC8I GCNNGC 2 cut(s) 430, 604
BstDEI CTNAG 7 cut(s) 5, 116, 129, 210, 408, 787, 854
BstDSI CCRYGG 2 cut(s) 198, 754
BstF5I GGATG 2 cut(s) 574, 732
BstFNI CGCG 1 cut(s) 1014
BstH2I RGCGCY 2 cut(s) 249, 401
BstHHI GCGC 2 cut(s) 248, 400
BstKTI GATC 3 cut(s) 91, 470, 861
BstMAI GTCTC 1 cut(s) 700
BstMBI GATC 3 cut(s) 88, 467, 858
BstMWI GCNNNNNNNGC 6 cut(s) 179, 404, 425, 741, 750, 771
BstNI CCWGG 5 cut(s) 566, 708, 750, 776, 987
BstPAI GACNNNNGTC 1 cut(s) 520
BstSCI CCNGG 6 cut(s) 564, 706, 748, 774, 884, 985
BstUI CGCG 1 cut(s) 1014
BstV1I GCAGC 3 cut(s) 70, 548, 586
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 5 cut(s) 254, 339, 705, 753, 779
BtgI CCRYGG 2 cut(s) 198, 754
BtgZI GCGATG 1 cut(s) 1029
BtsCI GGATG 2 cut(s) 574, 732
BtsI GCAGTG 2 cut(s) 1084, 1102
BtsIMutI CAGTG 2 cut(s) 1084, 1102
BtuMI TCGCGA 1 cut(s) 1014
Cac8I GCNNGC 2 cut(s) 430, 604
CfoI GCGC 2 cut(s) 248, 400
Cfr13I GGNCC 3 cut(s) 704, 711, 746
CseI GACGC 1 cut(s) 574
Csp6I GTAC 8 cut(s) 221, 332, 441, 572, 803, 840, 1019, 1024
CviAII CATG 4 cut(s) 174, 632, 741, 1092
CviQI GTAC 8 cut(s) 221, 332, 441, 572, 803, 840, 1019, 1024
DdeI CTNAG 7 cut(s) 5, 116, 129, 210, 408, 787, 854
DpnI GATC 3 cut(s) 90, 469, 860
DpnII GATC 3 cut(s) 88, 467, 858
DraI TTTAAA 1 cut(s) 259
DraIII CACNNNGTG 1 cut(s) 550
DrdI GACNNNNNNGTC 1 cut(s) 692
DseDI GACNNNNNNGTC 1 cut(s) 692
EaeI YGGCCR 2 cut(s) 751, 777
Eam1104I CTCTTC 1 cut(s) 786
EarI CTCTTC 1 cut(s) 786
EciI GGCGGA 1 cut(s) 406
Eco130I CCWWGG 2 cut(s) 714, 895
Eco47I GGWCC 2 cut(s) 711, 746
EcoRII CCWGG 5 cut(s) 564, 706, 748, 774, 985
EcoT14I CCWWGG 2 cut(s) 714, 895
EcoT22I ATGCAT 1 cut(s) 175
ErhI CCWWGG 2 cut(s) 714, 895
FaeI CATG 4 cut(s) 177, 635, 744, 1095
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 2 cut(s) 698, 724
FatI CATG 4 cut(s) 173, 631, 740, 1091
FauNDI CATATG 1 cut(s) 282
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 3 cut(s) 84, 562, 600
FokI GGATG 2 cut(s) 581, 739
Fsp4HI GCNGC 3 cut(s) 84, 562, 600
FspBI CTAG 2 cut(s) 335, 879
GlaI GCGC 2 cut(s) 247, 399
GluI GCNGC 3 cut(s) 84, 562, 600
HaeII RGCGCY 2 cut(s) 249, 401
HaeIII GGCC 5 cut(s) 254, 339, 705, 753, 779
HapII CCGG 2 cut(s) 885, 1132
HgaI GACGC 1 cut(s) 574
HhaI GCGC 2 cut(s) 248, 400
Hin1I GRCGYC 1 cut(s) 585
Hin1II CATG 4 cut(s) 177, 635, 744, 1095
Hin6I GCGC 2 cut(s) 246, 398
HinP1I GCGC 2 cut(s) 246, 398
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 5 cut(s) 16, 149, 203, 906, 1107
HpaII CCGG 2 cut(s) 885, 1132
HphI GGTGA 2 cut(s) 275, 309
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 93, 211, 294, 392
Hpy188III TCNNGA 3 cut(s) 115, 167, 1013
Hpy8I GTNNAC 1 cut(s) 382
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 3 cut(s) 220, 506, 791
HpyCH4III ACNGT 7 cut(s) 43, 140, 692, 817, 983, 1023, 1157
HpyCH4IV ACGT 1 cut(s) 810
HpyCH4V TGCA 9 cut(s) 173, 270, 432, 656, 827, 1061, 1077, 1082, 1095
HpyF10VI GCNNNNNNNGC 6 cut(s) 179, 404, 425, 741, 750, 771
HpyF3I CTNAG 7 cut(s) 5, 116, 129, 210, 408, 787, 854
HpySE526I ACGT 1 cut(s) 810
Hsp92I GRCGYC 1 cut(s) 585
Hsp92II CATG 4 cut(s) 177, 635, 744, 1095
HspAI GCGC 2 cut(s) 246, 398
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 3 cut(s) 88, 467, 858
LmnI GCTCC 2 cut(s) 125, 771
Lsp1109I GCAGC 3 cut(s) 70, 548, 586
LweI GCATC 2 cut(s) 160, 394
MaeI CTAG 2 cut(s) 335, 879
MaeII ACGT 1 cut(s) 810
MaeIII GTNAC 5 cut(s) 134, 142, 263, 370, 811
MalI GATC 3 cut(s) 90, 469, 860
MboI GATC 3 cut(s) 88, 467, 858
MboII GAAGA 2 cut(s) 652, 803
MflI RGATCY 1 cut(s) 467
MlsI TGGCCA 2 cut(s) 753, 779
MluCI AATT 8 cut(s) 434, 591, 638, 828, 948, 958, 1067, 1120
MluNI TGGCCA 2 cut(s) 753, 779
MlyI GAGTC 1 cut(s) 1101
MmeI TCCRAC 1 cut(s) 370
MnlI CCTC 9 cut(s) 205, 217, 225, 386, 679, 787, 849, 986, 1031
Mox20I TGGCCA 2 cut(s) 753, 779
Mph1103I ATGCAT 1 cut(s) 175
MscI TGGCCA 2 cut(s) 753, 779
MseI TTAA 5 cut(s) 258, 437, 819, 834, 927
Msp20I TGGCCA 2 cut(s) 753, 779
MspA1I CMGCKG 1 cut(s) 456
MspI CCGG 2 cut(s) 885, 1132
MspR9I CCNGG 6 cut(s) 566, 708, 750, 776, 886, 987
Mva1269I GAATGC 1 cut(s) 627
MvaI CCWGG 5 cut(s) 566, 708, 750, 776, 987
MvnI CGCG 1 cut(s) 1014
MwoI GCNNNNNNNGC 6 cut(s) 179, 404, 425, 741, 750, 771
NciI CCSGG 1 cut(s) 886
NdeI CATATG 1 cut(s) 282
NdeII GATC 3 cut(s) 88, 467, 858
NlaIII CATG 4 cut(s) 177, 635, 744, 1095
NlaIV GGNNCC 4 cut(s) 415, 469, 712, 773
NmuCI GTSAC 1 cut(s) 263
NruI TCGCGA 1 cut(s) 1014
NsiI ATGCAT 1 cut(s) 175
PasI CCCWGGG 1 cut(s) 707
PctI GAATGC 1 cut(s) 627
PfeI GAWTC 4 cut(s) 16, 149, 203, 906
PflFI GACNNNGTC 1 cut(s) 692
PflMI CCANNNNNTGG 1 cut(s) 1097
PkrI GCNGC 3 cut(s) 85, 563, 601
PleI GAGTC 1 cut(s) 1101
PpsI GAGTC 1 cut(s) 1101
PshAI GACNNNNGTC 1 cut(s) 520
Psp6I CCWGG 5 cut(s) 564, 706, 748, 774, 985
PspGI CCWGG 5 cut(s) 564, 706, 748, 774, 985
PspN4I GGNNCC 4 cut(s) 415, 469, 712, 773
PspPI GGNCC 3 cut(s) 704, 711, 746
PsuI RGATCY 1 cut(s) 467
PsyI GACNNNGTC 1 cut(s) 692
PvuII CAGCTG 1 cut(s) 456
RruI TCGCGA 1 cut(s) 1014
RsaI GTAC 8 cut(s) 222, 333, 442, 573, 804, 841, 1020, 1025
RsaNI GTAC 8 cut(s) 221, 332, 441, 572, 803, 840, 1019, 1024
SaqAI TTAA 5 cut(s) 258, 437, 819, 834, 927
SatI GCNGC 3 cut(s) 84, 562, 600
Sau3AI GATC 3 cut(s) 88, 467, 858
Sau96I GGNCC 3 cut(s) 704, 711, 746
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 1 cut(s) 1101
ScrFI CCNGG 6 cut(s) 566, 708, 750, 776, 886, 987
SfaNI GCATC 2 cut(s) 160, 394
SinI GGWCC 2 cut(s) 711, 746
Sse9I AATT 8 cut(s) 434, 591, 638, 828, 948, 958, 1067, 1120
SsiI CCGC 2 cut(s) 417, 744
SspMI CTAG 2 cut(s) 335, 879
StyD4I CCNGG 6 cut(s) 564, 706, 748, 774, 884, 985
StyI CCWWGG 2 cut(s) 714, 895
TaaI ACNGT 7 cut(s) 43, 140, 692, 817, 983, 1023, 1157
TaiI ACGT 1 cut(s) 813
TaqI TCGA 5 cut(s) 663, 904, 1036, 1046, 1110
TasI AATT 8 cut(s) 434, 591, 638, 828, 948, 958, 1067, 1120
TatI WGTACW 4 cut(s) 331, 571, 802, 1018
TfiI GAWTC 4 cut(s) 16, 149, 203, 906
Tru1I TTAA 5 cut(s) 258, 437, 819, 834, 927
Tru9I TTAA 5 cut(s) 258, 437, 819, 834, 927
TscAI CASTG 2 cut(s) 1084, 1102
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 3 cut(s) 83, 561, 599
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 2 cut(s) 620, 1175
TspGWI ACGGA 2 cut(s) 161, 215
TspRI CASTG 2 cut(s) 1084, 1102
Tth111I GACNNNGTC 1 cut(s) 692
Van91I CCANNNNNTGG 1 cut(s) 1097
VpaK11BI GGWCC 2 cut(s) 711, 746
XapI RAATTY 2 cut(s) 1067, 1120
XspI CTAG 2 cut(s) 335, 879
ZrmI AGTACT 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.