RLG00000018315

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001539
Physical Location & Seq
Reverse (-)
28231159 .. 28232661
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018315

Sequence Viewer

Length: 1206 bp
ATGGTGCAGCTAACAAGTTGTTTTTGCACACTTGCATTCATTCTTCTGCATATCTTATACCAGCCATCTATCGCTGCTGCTGCTGATGAGACCGCAACTATTTACAATGTCTTAAGCTTTGGAGCTAAACGCAATGGAGTAACTGATTCAACCCAAGCTTTTCTTGATGCATGGGCTGCTGCCTGTGCCTCCCTAGACTCAGCCGAGATAAATATACCAAAAGGGAGGTATTTGCTCAACTCTATGACCTTTAAAGGTGACTGCAAGAGCCCACAGATTACTTTCCGGATTGATGGAACGCTCGTAGCTTCACCTGATTACCGAGTCCTAGGCCAAGCCAACAATTGGCTCGGTTTTGAAGTTGTCAGCGGGGTAACCATTATTGGAGGAACACTTGACGCCAAGGGTTCTGCATTGTGGGCTTGCAAATTGAGCACCAAAACCTCCAATTGTCCTGATGGCGCCACGAGTTTGAGTTTTACGAACTCCAAAAACATTAGAGTCGAGGGGCTAATGTCATTGAACAGCCAGATGTACCACATTGTCATAAACGGCTGCCAAGATGTTCTCATTCAAGGCGTCAAAGTCATTGCTGCTGGCAATAGCCCAAACACCGATGGCATCCATGTCCAGTTCTCGAAAAATGTTGCAATCCTCGACACCTCCATCAAAACCGGGGACGATTGTGTCTCTATTGGTCCCGGCACCAAGGACTTATGGATGGAACACATATCATGCGGTCCTGGCCACGGCATTAGCATTGGGAGCCTAGCCAAGGACTTGGAAGAGGAAGGGGTACAAAATGTAACGGTTAAAGATGCGATTTTTAAGGGCACCCAAAATGGTGTGAGGATAAAGTCTTGGGCGAGACGTAGCAACGGGTTTGTCCAAGGTGTCCAATTTCTTGACGTTGAAATGGTTGATGTCCAAAACCCTATTGTAATTGACCAAAATTACTGCCCACACAACCTTAACTGCCCTGCTCAGGTATCAGGTGTGAAAATTAGTGATGTATTGTATCAAAACATTCGAGGAACATCAGCAACAGCAGTCGCAGTAAAATTTGCTTGTAGTGCAGCAAATCCTTGCAGTGGGATAAGACTCAAAAACGTGAATCTGGCTTGCCGGAATCAAGCTGTGCAATCATATTGTACGAATGCAATCGGAAAGGCTTACGGTACCGTTCAACCAAACAGTTGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

402

Amino Acids

42.69

Weight (kDa)

7.43

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 35 - 288 1.9e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 62 - 387 2.3e-86 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
Acc65I GGTACC 1 cut(s) 1178
AccB1I GGYRCC 4 cut(s) 461, 704, 833, 1178
AccB7I CCANNNNNTGG 1 cut(s) 345
AccIII TCCGGA 1 cut(s) 285
AciI CCGC 3 cut(s) 93, 369, 738
AcoI YGGCCR 1 cut(s) 745
AcsI RAATTY 1 cut(s) 1061
AcyI GRCGYC 3 cut(s) 399, 462, 579
AfaI GTAC 4 cut(s) 536, 798, 1153, 1180
AfiI CCNNNNNNNGG 5 cut(s) 345, 749, 775, 985, 1091
AflII CTTAAG 1 cut(s) 112
AgsI TTSAA 6 cut(s) 150, 359, 523, 575, 914, 1187
AjnI CCWGG 1 cut(s) 742
AluBI AGCT 6 cut(s) 10, 117, 125, 158, 308, 1136
AluI AGCT 6 cut(s) 10, 117, 125, 158, 308, 1136
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 3 cut(s) 83, 694, 862
Aor13HI TCCGGA 1 cut(s) 285
AoxI GGCC 2 cut(s) 331, 745
ApeKI GCWGC 9 cut(s) 7, 74, 77, 80, 176, 179, 555, 593, 1076
ApoI RAATTY 1 cut(s) 1061
Asp718I GGTACC 1 cut(s) 1178
AspA2I CCTAGG 1 cut(s) 328
AspLEI GCGC 1 cut(s) 464
AspS9I GGNCC 2 cut(s) 698, 740
AsuC2I CCSGG 2 cut(s) 676, 702
AsuHPI GGTGA 2 cut(s) 269, 303
AvaII GGWCC 2 cut(s) 698, 740
AvrII CCTAGG 1 cut(s) 328
BaeGI GKGCMC 1 cut(s) 836
BalI TGGCCA 1 cut(s) 747
BanI GGYRCC 4 cut(s) 461, 704, 833, 1178
BanII GRGCYC 1 cut(s) 272
BauI CACGAG 1 cut(s) 466
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 9 cut(s) 19, 61, 64, 67, 163, 166, 542, 580, 1088
BccI CCATC 6 cut(s) 73, 287, 452, 611, 674, 715
BceAI ACGGC 2 cut(s) 568, 766
BciT130I CCWGG 1 cut(s) 744
BcnI CCSGG 2 cut(s) 676, 702
BcoDI GTCTC 3 cut(s) 83, 694, 862
BfaI CTAG 3 cut(s) 194, 329, 770
BfoI RGCGCY 1 cut(s) 465
BfrI CTTAAG 1 cut(s) 112
BisI GCNGC 9 cut(s) 8, 75, 78, 81, 177, 180, 556, 594, 1077
BlnI CCTAGG 1 cut(s) 328
BlsI GCNGC 9 cut(s) 9, 76, 79, 82, 178, 181, 557, 595, 1078
Bme1390I CCNGG 3 cut(s) 676, 702, 744
Bme18I GGWCC 2 cut(s) 698, 740
BmgT120I GGNCC 2 cut(s) 698, 740
BmiI GGNNCC 6 cut(s) 463, 700, 706, 767, 835, 1180
BmrFI CCNGG 3 cut(s) 676, 702, 744
BmsI GCATC 3 cut(s) 157, 630, 808
Bpu10I CCTNAGC 1 cut(s) 984
BpuMI CCSGG 2 cut(s) 676, 702
BsaHI GRCGYC 3 cut(s) 399, 462, 579
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 7 cut(s) 328, 402, 675, 708, 748, 774, 889
BsaWI WCCGGW 1 cut(s) 285
Bsc4I CCNNNNNNNGG 5 cut(s) 345, 749, 775, 985, 1091
Bse1I ACTGG 1 cut(s) 631
Bse3DI GCAATG 2 cut(s) 139, 588
BseAI TCCGGA 1 cut(s) 285
BseBI CCWGG 1 cut(s) 744
BseDI CCNNGG 7 cut(s) 328, 402, 675, 708, 748, 774, 889
BseGI GGATG 2 cut(s) 621, 726
BseLI CCNNNNNNNGG 5 cut(s) 345, 749, 775, 985, 1091
BseMI GCAATG 2 cut(s) 139, 588
BseMII CTCAG 2 cut(s) 213, 998
BseNI ACTGG 1 cut(s) 631
BseSI GKGCMC 1 cut(s) 836
BseXI GCAGC 9 cut(s) 19, 61, 64, 67, 163, 166, 542, 580, 1088
BsgI GTGCAG 2 cut(s) 26, 1095
BshFI GGCC 2 cut(s) 333, 747
BshNI GGYRCC 4 cut(s) 461, 704, 833, 1178
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 4 cut(s) 286, 675, 702, 1126
BslFI GGGAC 2 cut(s) 684, 692
BslI CCNNNNNNNGG 5 cut(s) 345, 749, 775, 985, 1091
BsmAI GTCTC 3 cut(s) 83, 694, 862
BsmBI CGTCTC 1 cut(s) 862
BsmFI GGGAC 2 cut(s) 684, 692
BsmI GAATGC 2 cut(s) 35, 1162
BsnI GGCC 2 cut(s) 333, 747
Bso31I GGTCTC 1 cut(s) 83
Bsp1286I GDGCHC 3 cut(s) 272, 437, 836
Bsp13I TCCGGA 1 cut(s) 285
BspACI CCGC 3 cut(s) 93, 369, 738
BspANI GGCC 2 cut(s) 333, 747
BspCNI CTCAG 2 cut(s) 212, 997
BspEI TCCGGA 1 cut(s) 285
BspLI GGNNCC 6 cut(s) 463, 700, 706, 767, 835, 1180
BspT107I GGYRCC 4 cut(s) 461, 704, 833, 1178
BspTI CTTAAG 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 139, 588
BsrI ACTGG 1 cut(s) 631
BssECI CCNNGG 7 cut(s) 328, 402, 675, 708, 748, 774, 889
BssNI GRCGYC 3 cut(s) 399, 462, 579
BssSI CACGAG 1 cut(s) 466
BssT1I CCWWGG 5 cut(s) 328, 402, 708, 774, 889
Bst2BI CACGAG 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 744
Bst4CI ACNGT 4 cut(s) 811, 1178, 1183, 1196
Bst6I CTCTTC 1 cut(s) 780
BstACI GRCGYC 3 cut(s) 399, 462, 579
BstAFI CTTAAG 1 cut(s) 112
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 3 cut(s) 424, 598, 1123
BstDEI CTNAG 2 cut(s) 199, 984
BstDSI CCRYGG 1 cut(s) 748
BstEII GGTNACC 1 cut(s) 373
BstENI CCTNNNNNAGG 1 cut(s) 773
BstF5I GGATG 2 cut(s) 621, 726
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 1 cut(s) 464
BstMAI GTCTC 3 cut(s) 83, 694, 862
BstMWI GCNNNNNNNGC 8 cut(s) 80, 176, 185, 419, 432, 744, 765, 1073
BstNI CCWGG 1 cut(s) 744
BstPI GGTNACC 1 cut(s) 373
BstSCI CCNGG 3 cut(s) 674, 700, 742
BstSLI GKGCMC 1 cut(s) 836
BstV1I GCAGC 9 cut(s) 19, 61, 64, 67, 163, 166, 542, 580, 1088
BstXI CCANNNNNNTGG 1 cut(s) 781
BsuRI GGCC 2 cut(s) 333, 747
BtgI CCRYGG 1 cut(s) 748
BtsCI GGATG 2 cut(s) 621, 726
BtsI GCAGTG 1 cut(s) 1096
BtsIMutI CAGTG 1 cut(s) 1096
Cac8I GCNNGC 3 cut(s) 424, 598, 1123
CfoI GCGC 1 cut(s) 464
Cfr13I GGNCC 2 cut(s) 698, 740
CseI GACGC 2 cut(s) 407, 568
Csp6I GTAC 4 cut(s) 535, 797, 1152, 1179
CviAII CATG 3 cut(s) 171, 626, 735
CviQI GTAC 4 cut(s) 535, 797, 1152, 1179
DdeI CTNAG 2 cut(s) 199, 984
DinI GGCGCC 1 cut(s) 463
DraI TTTAAA 1 cut(s) 253
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 1 cut(s) 745
Eam1104I CTCTTC 1 cut(s) 780
EarI CTCTTC 1 cut(s) 780
Eco130I CCWWGG 5 cut(s) 328, 402, 708, 774, 889
Eco24I GRGCYC 1 cut(s) 272
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 698, 740
Eco91I GGTNACC 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 773
EcoO65I GGTNACC 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 742
EcoT14I CCWWGG 5 cut(s) 328, 402, 708, 774, 889
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 272
EgeI GGCGCC 1 cut(s) 463
EheI GGCGCC 1 cut(s) 463
ErhI CCWWGG 5 cut(s) 328, 402, 708, 774, 889
Esp3I CGTCTC 1 cut(s) 862
FaeI CATG 3 cut(s) 174, 629, 738
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FaqI GGGAC 2 cut(s) 684, 692
FatI CATG 3 cut(s) 170, 625, 734
FauI CCCGC 1 cut(s) 362
Fnu4HI GCNGC 9 cut(s) 8, 75, 78, 81, 177, 180, 556, 594, 1077
FokI GGATG 2 cut(s) 608, 733
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 9 cut(s) 8, 75, 78, 81, 177, 180, 556, 594, 1077
FspBI CTAG 3 cut(s) 194, 329, 770
GlaI GCGC 1 cut(s) 463
GluI GCNGC 9 cut(s) 8, 75, 78, 81, 177, 180, 556, 594, 1077
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 2 cut(s) 333, 747
HapII CCGG 4 cut(s) 286, 675, 702, 1126
HgaI GACGC 2 cut(s) 407, 568
HhaI GCGC 1 cut(s) 464
Hin1I GRCGYC 3 cut(s) 399, 462, 579
Hin1II CATG 3 cut(s) 174, 629, 738
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HindIII AAGCTT 2 cut(s) 115, 156
HinfI GANTC 7 cut(s) 146, 197, 324, 501, 1101, 1114, 1129
HpaII CCGG 4 cut(s) 286, 675, 702, 1126
HphI GGTGA 2 cut(s) 269, 303
Hpy188I TCNGA 1 cut(s) 1166
Hpy188III TCNNGA 5 cut(s) 164, 286, 455, 637, 905
HpyAV CCTTC 1 cut(s) 785
HpyCH4III ACNGT 4 cut(s) 811, 1178, 1183, 1196
HpyCH4IV ACGT 3 cut(s) 871, 909, 1110
HpyF10VI GCNNNNNNNGC 8 cut(s) 80, 176, 185, 419, 432, 744, 765, 1073
HpyF3I CTNAG 2 cut(s) 199, 984
HpySE526I ACGT 3 cut(s) 871, 909, 1110
Hsp92I GRCGYC 3 cut(s) 399, 462, 579
Hsp92II CATG 3 cut(s) 174, 629, 738
HspAI GCGC 1 cut(s) 462
KasI GGCGCC 1 cut(s) 461
Kpn2I TCCGGA 1 cut(s) 285
KpnI GGTACC 1 cut(s) 1182
LmnI GCTCC 2 cut(s) 122, 765
Lsp1109I GCAGC 9 cut(s) 19, 61, 64, 67, 163, 166, 542, 580, 1088
LweI GCATC 3 cut(s) 157, 630, 808
MaeI CTAG 3 cut(s) 194, 329, 770
MaeII ACGT 3 cut(s) 871, 909, 1110
MaeIII GTNAC 4 cut(s) 139, 257, 373, 805
MboII GAAGA 2 cut(s) 35, 797
MfeI CAATTG 2 cut(s) 343, 448
MhlI GDGCHC 3 cut(s) 272, 437, 836
MlsI TGGCCA 1 cut(s) 747
MluCI AATT 8 cut(s) 343, 428, 448, 899, 942, 952, 1002, 1061
MluNI TGGCCA 1 cut(s) 747
Mly113I GGCGCC 1 cut(s) 462
MlyI GAGTC 4 cut(s) 191, 333, 510, 1095
Mox20I TGGCCA 1 cut(s) 747
Mph1103I ATGCAT 1 cut(s) 172
MroI TCCGGA 1 cut(s) 285
MscI TGGCCA 1 cut(s) 747
MseI TTAA 5 cut(s) 113, 252, 813, 828, 972
Msp20I TGGCCA 1 cut(s) 747
MspA1I CMGCKG 1 cut(s) 369
MspCI CTTAAG 1 cut(s) 112
MspI CCGG 4 cut(s) 286, 675, 702, 1126
MspR9I CCNGG 3 cut(s) 676, 702, 744
MunI CAATTG 2 cut(s) 343, 448
Mva1269I GAATGC 2 cut(s) 35, 1162
MvaI CCWGG 1 cut(s) 744
MwoI GCNNNNNNNGC 8 cut(s) 80, 176, 185, 419, 432, 744, 765, 1073
NarI GGCGCC 1 cut(s) 462
NciI CCSGG 2 cut(s) 676, 702
NlaIII CATG 3 cut(s) 174, 629, 738
NlaIV GGNNCC 6 cut(s) 463, 700, 706, 767, 835, 1180
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 257
NsiI ATGCAT 1 cut(s) 172
PctI GAATGC 2 cut(s) 35, 1162
PfeI GAWTC 3 cut(s) 146, 1114, 1129
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 9 cut(s) 9, 76, 79, 82, 178, 181, 557, 595, 1078
PleI GAGTC 4 cut(s) 191, 332, 509, 1095
PluTI GGCGCC 1 cut(s) 465
PpsI GAGTC 4 cut(s) 191, 332, 509, 1095
Psp6I CCWGG 1 cut(s) 742
PspEI GGTNACC 1 cut(s) 373
PspGI CCWGG 1 cut(s) 742
PspN4I GGNNCC 6 cut(s) 463, 700, 706, 767, 835, 1180
PspPI GGNCC 2 cut(s) 698, 740
RsaI GTAC 4 cut(s) 536, 798, 1153, 1180
RsaNI GTAC 4 cut(s) 535, 797, 1152, 1179
SaqAI TTAA 5 cut(s) 113, 252, 813, 828, 972
SatI GCNGC 9 cut(s) 8, 75, 78, 81, 177, 180, 556, 594, 1077
Sau96I GGNCC 2 cut(s) 698, 740
SchI GAGTC 4 cut(s) 191, 333, 510, 1095
ScrFI CCNGG 3 cut(s) 676, 702, 744
SduI GDGCHC 3 cut(s) 272, 437, 836
SfaNI GCATC 3 cut(s) 157, 630, 808
SfoI GGCGCC 1 cut(s) 463
SinI GGWCC 2 cut(s) 698, 740
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 8 cut(s) 343, 428, 448, 899, 942, 952, 1002, 1061
SsiI CCGC 3 cut(s) 93, 369, 738
SspDI GGCGCC 1 cut(s) 461
SspMI CTAG 3 cut(s) 194, 329, 770
StyD4I CCNGG 3 cut(s) 674, 700, 742
StyI CCWWGG 5 cut(s) 328, 402, 708, 774, 889
TaaI ACNGT 4 cut(s) 811, 1178, 1183, 1196
TaiI ACGT 3 cut(s) 874, 912, 1113
TaqI TCGA 4 cut(s) 504, 638, 657, 1030
TasI AATT 8 cut(s) 343, 428, 448, 899, 942, 952, 1002, 1061
TfiI GAWTC 3 cut(s) 146, 1114, 1129
Tru1I TTAA 5 cut(s) 113, 252, 813, 828, 972
Tru9I TTAA 5 cut(s) 113, 252, 813, 828, 972
TscAI CASTG 1 cut(s) 1096
TseFI GTSAC 1 cut(s) 257
TseI GCWGC 9 cut(s) 7, 74, 77, 80, 176, 179, 555, 593, 1076
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 1 cut(s) 28
TspRI CASTG 1 cut(s) 1096
Van91I CCANNNNNTGG 1 cut(s) 345
Vha464I CTTAAG 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 698, 740
XagI CCTNNNNNAGG 1 cut(s) 773
XapI RAATTY 1 cut(s) 1061
XmaJI CCTAGG 1 cut(s) 328
XspI CTAG 3 cut(s) 194, 329, 770
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.