Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001539

Physical Location & Seq

Genomic Coordinates Forward (+)

13538362 .. 13539962

Gene Structure 1601 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1096500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1209 bp

ATGGATGAGGCTGCAAGTTGTTTTTCCAGAGCTAGCGTAGTACCAATCATGATTCTCTTTATCTTACACCAATCATTTATTGCTGAGAGTACTACATTAGTTCTCAATATCCTGAGCTTCGGAGCTAAGCCCAATGGAGTAACCGATTCCACCCAAGCTTTTATGGATGCATGGAGTGCAGCTTGTGCCTCTTCGGATTCCAGTACGATTAGGGTACCAAAAGGGATGTATTTGCTTCCGACTGCTATGGTTTTTAAAGGTGACAACTGCAAAAGCCCAGGTATTACTTTTTGGATTGATGGAACTCTCGTCGCTTCGTCTGACTACCAAATTCTTGGCCAAGCTGACAATTGGCTAAGCTTCGAAACAGTTACCGGCGTTTCCATTATTGGAGGTGCACTTGACGCCAAAGGCTCCTCCTTGTGGGCATGCAAGTTAGCGGGAAGCACTGATTGCCCCAGCGGAGCAACGAGTTTGAGTTTTACAAACTCCAAAAACATTAGGATCGACGGACTAATGTCGTTGAACAGCCAGATGTACCACATTGTTATAAACGGGTGCCAAGATGTTCACATTCAAGGAGTCAAAATCATTGCCGCCGGCAATAGCCCCAACACTGACGGCATCCATGTTCAATTGTCAAGAAATGTTGCAATCTTCGACACCTCCATCAAAACGGGAGATGACTGCGTCTCAATTGGCCCCGGCACCAAGGACTTGTGGATCGAGCACATATCGTGTGGACCAAGTCACGGCATTAGCATAGGGAGTTTGGGCAAAGACTTGGAAGAGGAAGGAGTTCAAAATGTGACAGTGAAGGATGCAATTTTCAAGGGTGGCCAAAATGGTTTGAGGATTAAGTCTTGGGCAAGGCCGAGCCACGGGTTTGTTCAAAGTGTCCAATTCCTTGACGTCGTCATGCACAATGTCCAAAATCCCATTATCATAGACCAAAATTACTGCCCACACAACGTCAACTGCCCTGGTCAGGTATCGGGTGTGAAAGTCAGTGATGTTTTGTTCCGAAACATTCAAGGAACATCGGCAACAGCAATCGCGGTAAATCTTGATTGCAGTGCGGCAAATCCTTGCAGAGGGATAAAACTCGAAAACGTGAGCTTGACCTGCAAGAAGCAAGTGGCTCAGTCATATTGTAGCAATGCAATTGGAAAGACTTCTGGCCTTGTACACCCAACTAGTTGTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Glyco_hydro_28

Protein Analysis

403

Amino Acids

42.55

Weight (kDa)

6.18

Isoelectric Point (pI)

38.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	35 - 289	2e-08	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	62 - 388	6.4e-82	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001539)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g22630
malus_domestica	MD12G1096500.v1.1
prunus_persica	Prupe.7G005500_v2.0.a1 Prupe.7G030700_v2.0.a1 Prupe.7G031900_v2.0.a1 Prupe.7G036000_v2.0.a1 Prupe.7G036300_v2.0.a1
pyrus_communis	pycom12g08540 pycom12g08550 pycom14g00130
rosa_chinensis	RchiOBHm_Chr2g0116641 RchiOBHm_Chr2g0116851 RchiOBHm_Chr2g0116891 RchiOBHm_Chr2g0116931 RchiOBHm_Chr2g0116951
rosa_laevigata	RLG00000018296 RLG00000018300 RLG00000018301 RLG00000018305 RLG00000018307 RLG00000018315 RLG00000032066
rosa_multiflora	Rmu_co8325457.1_g000001 Rmu_co8398115.1_g000001 Rmu_co8400735.1_g000001 Rmu_co8406615.1_g000001 Rmu_co8465195.1_g000001 Rmu_sc0002177.1_g000015 Rmu_sc0004942.1_g000013 Rmu_sc0011969.1_g000016 Rmu_sc0013102.1_g000010 Rmu_sc0016190.1_g000004 Rmu_ssc0000141.1_g000016 Rmu_ssc0000141.1_g000018 Rmu_ssc0000238.1_g000024 Rmu_ssc0000238.1_g000031
rosa_roxburghii	Rroxscaffold_2G00126930 Rroxscaffold_2G00126990 Rroxscaffold_6G00390380 Rroxscaffold_6G00390440 Rroxscaffold_6G00390460
rosa_rugosa	Rorug02G0208600 Rorug02G0208900 Rorug02G0208900
rosa_samantha	Rh2BG275400 Rh2BG275800 Rh2BG276100 Rh2BG276700
rosa_wichuraiana	Rw2G020840 Rw2G020860 Rw2G020890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	551
AatII	GACGTC	1 cut(s)	915
Acc36I	ACCTGC	1 cut(s)	1133
Acc65I	GGTACC	1 cut(s)	214
AccB1I	GGYRCC	3 cut(s)	214, 558, 707
AccII	CGCG	1 cut(s)	1058
AciI	CCGC	5 cut(s)	440, 462, 597, 1058, 1079
AclWI	GGATC	2 cut(s)	512, 731
AcoI	YGGCCR	2 cut(s)	337, 838
AcsI	RAATTY	1 cut(s)	330
AcyI	GRCGYC	2 cut(s)	405, 912
AfaI	GTAC	6 cut(s)	42, 91, 205, 216, 539, 1188
AfiI	CCNNNNNNNGG	5 cut(s)	423, 752, 881, 988, 1094
AgsI	TTSAA	7 cut(s)	526, 578, 635, 803, 832, 893, 1034
AhlI	ACTAGT	1 cut(s)	1196
AjnI	CCWGG	2 cut(s)	277, 982
AluBI	AGCT	8 cut(s)	32, 117, 125, 158, 182, 344, 360, 1119
AluI	AGCT	8 cut(s)	32, 117, 125, 158, 182, 344, 360, 1119
Alw21I	GWGCWC	2 cut(s)	400, 732
Alw26I	GTCTC	1 cut(s)	697
Alw44I	GTGCAC	1 cut(s)	396
AlwI	GGATC	2 cut(s)	512, 731
AoxI	GGCC	5 cut(s)	337, 700, 838, 872, 1180
ApaLI	GTGCAC	1 cut(s)	396
ApeKI	GCWGC	2 cut(s)	11, 179
ApoI	RAATTY	1 cut(s)	330
Asp700I	GAANNNNTTC	2 cut(s)	798, 1174
Asp718I	GGTACC	1 cut(s)	214
AspS9I	GGNCC	2 cut(s)	701, 743
AsuC2I	CCSGG	1 cut(s)	705
AsuHPI	GGTGA	1 cut(s)	272
AsuII	TTCGAA	1 cut(s)	363
AsuNHI	GCTAGC	1 cut(s)	32
AvaII	GGWCC	1 cut(s)	743
BaeGI	GKGCMC	1 cut(s)	400
BalI	TGGCCA	2 cut(s)	339, 840
BanI	GGYRCC	3 cut(s)	214, 558, 707
Bbv12I	GWGCWC	2 cut(s)	400, 732
BbvI	GCAGC	1 cut(s)	191
BccI	CCATC	2 cut(s)	293, 677
BceAI	ACGGC	2 cut(s)	637, 769
BciT130I	CCWGG	2 cut(s)	279, 984
BcnI	CCSGG	1 cut(s)	705
BcoDI	GTCTC	1 cut(s)	697
BcuI	ACTAGT	1 cut(s)	1196
BfaI	CTAG	2 cut(s)	33, 1197
BfuAI	ACCTGC	1 cut(s)	1133
BisI	GCNGC	4 cut(s)	12, 180, 597, 1080
BlpI	GCTNAGC	2 cut(s)	126, 356
BlsI	GCNGC	4 cut(s)	13, 181, 598, 1081
BmcAI	AGTACT	1 cut(s)	91
Bme1390I	CCNGG	3 cut(s)	279, 705, 984
Bme18I	GGWCC	1 cut(s)	743
BmgT120I	GGNCC	2 cut(s)	701, 743
BmiI	GGNNCC	5 cut(s)	216, 415, 560, 703, 709
BmrFI	CCNGG	3 cut(s)	279, 705, 984
BmsI	GCATC	3 cut(s)	157, 633, 811
BmtI	GCTAGC	1 cut(s)	36
BoxI	GACNNNNGTC	1 cut(s)	517
Bpu10I	CCTNAGC	1 cut(s)	113
Bpu1102I	GCTNAGC	2 cut(s)	126, 356
Bpu14I	TTCGAA	1 cut(s)	363
BpuMI	CCSGG	1 cut(s)	705
BsaHI	GRCGYC	2 cut(s)	405, 912
BsaJI	CCNNGG	5 cut(s)	277, 703, 711, 880, 982
Bsc4I	CCNNNNNNNGG	5 cut(s)	423, 752, 881, 988, 1094
Bse118I	RCCGGY	2 cut(s)	374, 599
Bse1I	ACTGG	1 cut(s)	201
Bse3DI	GCAATG	2 cut(s)	591, 1165
BseBI	CCWGG	2 cut(s)	279, 984
BseDI	CCNNGG	5 cut(s)	277, 703, 711, 880, 982
BseGI	GGATG	5 cut(s)	10, 172, 231, 624, 826
BseLI	CCNNNNNNNGG	5 cut(s)	423, 752, 881, 988, 1094
BseMI	GCAATG	2 cut(s)	591, 1165
BseMII	CTCAG	3 cut(s)	75, 104, 1157
BseNI	ACTGG	1 cut(s)	201
BseRI	GAGGAG	1 cut(s)	406
BseSI	GKGCMC	1 cut(s)	400
BseXI	GCAGC	1 cut(s)	191
BseYI	CCCAGC	1 cut(s)	458
BsgI	GTGCAG	1 cut(s)	198
Bsh1236I	CGCG	1 cut(s)	1058
BshFI	GGCC	5 cut(s)	339, 702, 840, 874, 1182
BshNI	GGYRCC	3 cut(s)	214, 558, 707
BsiHKAI	GWGCWC	2 cut(s)	400, 732
BsiSI	CCGG	3 cut(s)	375, 600, 705
BslI	CCNNNNNNNGG	5 cut(s)	423, 752, 881, 988, 1094
BsmAI	GTCTC	1 cut(s)	697
BsmBI	CGTCTC	1 cut(s)	697
BsnI	GGCC	5 cut(s)	339, 702, 840, 874, 1182
Bsp119I	TTCGAA	1 cut(s)	363
Bsp1286I	GDGCHC	2 cut(s)	400, 732
Bsp1407I	TGTACA	1 cut(s)	1186
Bsp143I	GATC	2 cut(s)	504, 723
Bsp1720I	GCTNAGC	2 cut(s)	126, 356
BspACI	CCGC	5 cut(s)	440, 462, 597, 1058, 1079
BspANI	GGCC	5 cut(s)	339, 702, 840, 874, 1182
BspCNI	CTCAG	3 cut(s)	76, 105, 1156
BspFNI	CGCG	1 cut(s)	1058
BspHI	TCATGA	1 cut(s)	48
BspLI	GGNNCC	5 cut(s)	216, 415, 560, 703, 709
BspMI	ACCTGC	1 cut(s)	1133
BspOI	GCTAGC	1 cut(s)	36
BspPI	GGATC	2 cut(s)	512, 731
BspT104I	TTCGAA	1 cut(s)	363
BspT107I	GGYRCC	3 cut(s)	214, 558, 707
BsrDI	GCAATG	2 cut(s)	591, 1165
BsrFI	RCCGGY	2 cut(s)	374, 599
BsrGI	TGTACA	1 cut(s)	1186
BsrI	ACTGG	1 cut(s)	201
BssAI	RCCGGY	2 cut(s)	374, 599
BssECI	CCNNGG	5 cut(s)	277, 703, 711, 880, 982
BssMI	GATC	2 cut(s)	504, 723
BssNI	GRCGYC	2 cut(s)	405, 912
BssT1I	CCWWGG	1 cut(s)	711
Bst2UI	CCWGG	2 cut(s)	279, 984
Bst4CI	ACNGT	2 cut(s)	370, 814
Bst6I	CTCTTC	2 cut(s)	196, 783
BstACI	GRCGYC	2 cut(s)	405, 912
BstAPI	GCANNNNNTGC	3 cut(s)	176, 185, 453
BstAUI	TGTACA	1 cut(s)	1186
BstBI	TTCGAA	1 cut(s)	363
BstC8I	GCNNGC	3 cut(s)	34, 430, 601
BstDEI	CTNAG	5 cut(s)	84, 113, 126, 356, 1143
BstDSI	CCRYGG	1 cut(s)	880
BstENI	CCTNNNNNAGG	1 cut(s)	1092
BstF5I	GGATG	5 cut(s)	10, 172, 231, 624, 826
BstFNI	CGCG	1 cut(s)	1058
BstKTI	GATC	2 cut(s)	507, 726
BstMAI	GTCTC	1 cut(s)	697
BstMBI	GATC	2 cut(s)	504, 723
BstMWI	GCNNNNNNNGC	5 cut(s)	176, 185, 404, 453, 1125
BstNI	CCWGG	2 cut(s)	279, 984
BstNSI	RCATGY	1 cut(s)	432
BstPAI	GACNNNNGTC	1 cut(s)	517
BstSCI	CCNGG	3 cut(s)	277, 703, 982
BstSLI	GKGCMC	1 cut(s)	400
BstUI	CGCG	1 cut(s)	1058
BstV1I	GCAGC	1 cut(s)	191
BstXI	CCANNNNNNTGG	1 cut(s)	335
BsuRI	GGCC	5 cut(s)	339, 702, 840, 874, 1182
BtgI	CCRYGG	1 cut(s)	880
BtsCI	GGATG	5 cut(s)	10, 172, 231, 624, 826
BtsI	GCAGTG	1 cut(s)	1081
BtsIMutI	CAGTG	5 cut(s)	447, 615, 819, 1015, 1081
BveI	ACCTGC	1 cut(s)	1133
Cac8I	GCNNGC	3 cut(s)	34, 430, 601
CciI	TCATGA	1 cut(s)	48
Cfr10I	RCCGGY	2 cut(s)	374, 599
Cfr13I	GGNCC	2 cut(s)	701, 743
CseI	GACGC	2 cut(s)	413, 679
Csp6I	GTAC	6 cut(s)	41, 90, 204, 215, 538, 1187
CviAII	CATG	5 cut(s)	49, 171, 429, 629, 919
CviQI	GTAC	6 cut(s)	41, 90, 204, 215, 538, 1187
DdeI	CTNAG	5 cut(s)	84, 113, 126, 356, 1143
DpnI	GATC	2 cut(s)	506, 725
DpnII	GATC	2 cut(s)	504, 723
DraI	TTTAAA	1 cut(s)	256
EaeI	YGGCCR	2 cut(s)	337, 838
Eam1104I	CTCTTC	2 cut(s)	196, 783
EarI	CTCTTC	2 cut(s)	196, 783
Eco130I	CCWWGG	1 cut(s)	711
Eco47I	GGWCC	1 cut(s)	743
EcoNI	CCTNNNNNAGG	1 cut(s)	1092
EcoRII	CCWGG	2 cut(s)	277, 982
EcoT14I	CCWWGG	1 cut(s)	711
EcoT22I	ATGCAT	1 cut(s)	172
ErhI	CCWWGG	1 cut(s)	711
Esp3I	CGTCTC	1 cut(s)	697
FaeI	CATG	5 cut(s)	52, 174, 432, 632, 922
FatI	CATG	5 cut(s)	48, 170, 428, 628, 918
FauI	CCCGC	1 cut(s)	433
Fnu4HI	GCNGC	4 cut(s)	12, 180, 597, 1080
FokI	GGATG	5 cut(s)	17, 179, 238, 611, 833
Fsp4HI	GCNGC	4 cut(s)	12, 180, 597, 1080
FspBI	CTAG	2 cut(s)	33, 1197
GluI	GCNGC	4 cut(s)	12, 180, 597, 1080
GsaI	CCCAGC	1 cut(s)	462
HaeIII	GGCC	5 cut(s)	339, 702, 840, 874, 1182
HapII	CCGG	3 cut(s)	375, 600, 705
HgaI	GACGC	2 cut(s)	413, 679
Hin1I	GRCGYC	2 cut(s)	405, 912
Hin1II	CATG	5 cut(s)	52, 174, 432, 632, 922
HincII	GTYRAC	1 cut(s)	976
HindII	GTYRAC	1 cut(s)	976
HindIII	AAGCTT	2 cut(s)	156, 358
HinfI	GANTC	4 cut(s)	52, 146, 197, 582
HpaII	CCGG	3 cut(s)	375, 600, 705
HphI	GGTGA	1 cut(s)	272
Hpy166II	GTNNAC	5 cut(s)	398, 571, 743, 976, 1189
Hpy188I	TCNGA	5 cut(s)	122, 196, 240, 322, 1025
Hpy188III	TCNNGA	5 cut(s)	27, 49, 112, 642, 1067
Hpy8I	GTNNAC	5 cut(s)	398, 571, 743, 976, 1189
Hpy99I	CGWCG	3 cut(s)	314, 512, 917
HpyAV	CCTTC	2 cut(s)	788, 811
HpyCH4III	ACNGT	2 cut(s)	370, 814
HpyCH4IV	ACGT	3 cut(s)	912, 972, 1113
HpyF10VI	GCNNNNNNNGC	5 cut(s)	176, 185, 404, 453, 1125
HpyF3I	CTNAG	5 cut(s)	84, 113, 126, 356, 1143
HpySE526I	ACGT	3 cut(s)	912, 972, 1113
Hsp92I	GRCGYC	2 cut(s)	405, 912
Hsp92II	CATG	5 cut(s)	52, 174, 432, 632, 922
KpnI	GGTACC	1 cut(s)	218
KroI	GCCGGC	1 cut(s)	599
KroNI	GCCGGC	1 cut(s)	601
Kzo9I	GATC	2 cut(s)	504, 723
LmnI	GCTCC	3 cut(s)	122, 419, 464
Lsp1109I	GCAGC	1 cut(s)	191
LweI	GCATC	3 cut(s)	157, 633, 811
MaeI	CTAG	2 cut(s)	33, 1197
MaeII	ACGT	3 cut(s)	912, 972, 1113
MaeIII	GTNAC	5 cut(s)	139, 260, 370, 749, 808
MalI	GATC	2 cut(s)	506, 725
MboI	GATC	2 cut(s)	504, 723
MboII	GAAGA	3 cut(s)	183, 649, 800
MfeI	CAATTG	4 cut(s)	349, 635, 696, 1164
MhlI	GDGCHC	2 cut(s)	400, 732
MlsI	TGGCCA	2 cut(s)	339, 840
MluCI	AATT	8 cut(s)	330, 349, 635, 696, 825, 902, 955, 1164
MluNI	TGGCCA	2 cut(s)	339, 840
MlyI	GAGTC	1 cut(s)	591
MmeI	TCCRAC	1 cut(s)	263
MnlI	CCTC	7 cut(s)	199, 386, 427, 676, 784, 846, 1088
Mox20I	TGGCCA	2 cut(s)	339, 840
Mph1103I	ATGCAT	1 cut(s)	172
MroNI	GCCGGC	1 cut(s)	599
MroXI	GAANNNNTTC	2 cut(s)	798, 1174
MscI	TGGCCA	2 cut(s)	339, 840
MseI	TTAA	2 cut(s)	255, 858
Msp20I	TGGCCA	2 cut(s)	339, 840
MspA1I	CMGCKG	1 cut(s)	462
MspI	CCGG	3 cut(s)	375, 600, 705
MspR9I	CCNGG	3 cut(s)	279, 705, 984
MunI	CAATTG	4 cut(s)	349, 635, 696, 1164
MvaI	CCWGG	2 cut(s)	279, 984
MvnI	CGCG	1 cut(s)	1058
MwoI	GCNNNNNNNGC	5 cut(s)	176, 185, 404, 453, 1125
NaeI	GCCGGC	1 cut(s)	601
NciI	CCSGG	1 cut(s)	705
NdeII	GATC	2 cut(s)	504, 723
NgoMIV	GCCGGC	1 cut(s)	599
NheI	GCTAGC	1 cut(s)	32
NlaIII	CATG	5 cut(s)	52, 174, 432, 632, 922
NlaIV	GGNNCC	5 cut(s)	216, 415, 560, 703, 709
NmeAIII	GCCGAG	1 cut(s)	900
NmuCI	GTSAC	3 cut(s)	260, 749, 808
NsiI	ATGCAT	1 cut(s)	172
NspI	RCATGY	1 cut(s)	432
NspV	TTCGAA	1 cut(s)	363
PaeI	GCATGC	1 cut(s)	432
PagI	TCATGA	1 cut(s)	48
PdiI	GCCGGC	1 cut(s)	601
PdmI	GAANNNNTTC	2 cut(s)	798, 1174
PfeI	GAWTC	3 cut(s)	52, 146, 197
PflFI	GACNNNGTC	3 cut(s)	689, 747, 914
PkrI	GCNGC	4 cut(s)	13, 181, 598, 1081
PleI	GAGTC	1 cut(s)	590
PpsI	GAGTC	1 cut(s)	590
PshAI	GACNNNNGTC	1 cut(s)	517
PsiI	TTATAA	1 cut(s)	551
Psp6I	CCWGG	2 cut(s)	277, 982
PspFI	CCCAGC	1 cut(s)	458
PspGI	CCWGG	2 cut(s)	277, 982
PspN4I	GGNNCC	5 cut(s)	216, 415, 560, 703, 709
PspPI	GGNCC	2 cut(s)	701, 743
PsyI	GACNNNGTC	3 cut(s)	689, 747, 914
RsaI	GTAC	6 cut(s)	42, 91, 205, 216, 539, 1188
RsaNI	GTAC	6 cut(s)	41, 90, 204, 215, 538, 1187
SaqAI	TTAA	2 cut(s)	255, 858
SatI	GCNGC	4 cut(s)	12, 180, 597, 1080
Sau3AI	GATC	2 cut(s)	504, 723
Sau96I	GGNCC	2 cut(s)	701, 743
ScaI	AGTACT	1 cut(s)	91
SchI	GAGTC	1 cut(s)	591
ScrFI	CCNGG	3 cut(s)	279, 705, 984
SduI	GDGCHC	2 cut(s)	400, 732
SfaNI	GCATC	3 cut(s)	157, 633, 811
SfuI	TTCGAA	1 cut(s)	363
SinI	GGWCC	1 cut(s)	743
SpeI	ACTAGT	1 cut(s)	1196
SphI	GCATGC	1 cut(s)	432
Sse9I	AATT	8 cut(s)	330, 349, 635, 696, 825, 902, 955, 1164
SsiI	CCGC	5 cut(s)	440, 462, 597, 1058, 1079
SspMI	CTAG	2 cut(s)	33, 1197
StyD4I	CCNGG	3 cut(s)	277, 703, 982
StyI	CCWWGG	1 cut(s)	711
TaaI	ACNGT	2 cut(s)	370, 814
TaiI	ACGT	3 cut(s)	915, 975, 1116
TaqI	TCGA	5 cut(s)	363, 507, 660, 726, 1107
TasI	AATT	8 cut(s)	330, 349, 635, 696, 825, 902, 955, 1164
TatI	WGTACW	2 cut(s)	89, 1186
TauI	GCSGC	2 cut(s)	599, 1082
TfiI	GAWTC	3 cut(s)	52, 146, 197
Tru1I	TTAA	2 cut(s)	255, 858
Tru9I	TTAA	2 cut(s)	255, 858
TscAI	CASTG	5 cut(s)	454, 622, 819, 1015, 1081
TseFI	GTSAC	3 cut(s)	260, 749, 808
TseI	GCWGC	2 cut(s)	11, 179
Tsp45I	GTSAC	3 cut(s)	260, 749, 808
TspGWI	ACGGA	1 cut(s)	525
TspRI	CASTG	5 cut(s)	454, 622, 819, 1015, 1081
Tth111I	GACNNNGTC	3 cut(s)	689, 747, 914
VneI	GTGCAC	1 cut(s)	396
VpaK11BI	GGWCC	1 cut(s)	743
XagI	CCTNNNNNAGG	1 cut(s)	1092
XapI	RAATTY	1 cut(s)	330
XceI	RCATGY	1 cut(s)	432
XmnI	GAANNNNTTC	2 cut(s)	798, 1174
XspI	CTAG	2 cut(s)	33, 1197
ZraI	GACGTC	1 cut(s)	913
ZrmI	AGTACT	1 cut(s)	91
Zsp2I	ATGCAT	1 cut(s)	172

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.