RLG00000018300

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001539
Physical Location & Seq
Reverse (-)
28094787 .. 28096960
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018300

Sequence Viewer

Length: 1140 bp
ATGACTAAGCTTACAAGTTATTTTGCCACAGCTGTATTCATTTTTATACATATTCTATCCCAATCATCTACTTCGGGTGATAAGACGACTACAAGAACTTATAATGTTTCGGAGTTTGGAGCCAAGTGCAATGGTGTAACCAATTCAACCCAAGCTTTTCTCAATGCTTGGTCAGTAGCCTGTGGTTCCTCCGCAGATTCCACTGAGATATATGTACCAAAGGGGAGGTATTTGCTCGGCGCTATAGCCTTTAAAGGCGACTGCAAAAGCTCACACATCAATTTTCGGATTGATGGAACACTCGTGGCTTCACCTGATTACCGAGTACTAGGCCATGCTAACAACTGGTTCAGCTTTGAAGGAGTTAGCGGGATAACCATTGTCGGAGGAGCACTTGATGCCAAAGGTTCCGCCTTATGGGCTTGCAAATTAAGTACCAAAACCACCAGTTGTCCTGATGGAGCCACGACTTTGAGTTTTACAAACTCCAAGGACATTAGAGTTGAAGGACTAATGTCACTGAACAGCCAAATGTTTCACATTGTGATAAACGGCTGCCAAGATGTACTGATTCAAGGCGTCAAAGTCATTGCCGCTGGAAATAGCCCAAACACTGATGGTATCCATGTCCAGTTTTCCAGAAATGTTGCAATCTTCGACACCTCCATCAAAACTGGGGACGATTGTGTCTCTATTGGCCCTGGCACCAAGGACTCGTGGATGGAACGCATATCATGCGGACCTGGGCATGGCATTAGCATAGGGAGCCTGGCCAAAGACTTGGAAGAGGAAGGAGTCCAAAATGTAACACTCAAAAATGCGATTTTCAAGGGTACCACAAATGGTCTCAGGACAAAGTCGTGGGCAAGACCCAGCACGGGGTTTGTGCAAGGTGTCCGATTCCTTGATGTTGAAATGATTAAAGTCCAAAACCCCATTGTAATTGACCAGAATTACTGTCCACACAACCTCAACTGTCCTGGTCAGGTCTTCATGGTCCTGAGTTCAAAGAACCAAAATGCACACTCAGATTGGGAAATTCCAGCTGTGTTTGAGGGAACGTTGAAAGTCACTGCTTCTGGTAAAATTGAATATTACTCATCATCAAGCTTATTAAGCATAGATGGCCACGTGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

380

Amino Acids

40.62

Weight (kDa)

7.08

Isoelectric Point (pI)

25.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 35 - 79 3.6e-06 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 66 - 323 2.8e-66 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 102
AasI GACNNNNNNGTC 1 cut(s) 686
Acc65I GGTACC 1 cut(s) 833
AccB1I GGYRCC 2 cut(s) 704, 833
AciI CCGC 5 cut(s) 192, 369, 411, 594, 738
AclI AACGTT 1 cut(s) 1061
AcoI YGGCCR 2 cut(s) 771, 1126
AcsI RAATTY 1 cut(s) 1038
AcvI CACGTG 1 cut(s) 1132
AcyI GRCGYC 1 cut(s) 579
AdeI CACNNNGTG 1 cut(s) 544
AfaI GTAC 5 cut(s) 216, 327, 436, 567, 835
AfiI CCNNNNNNNGG 4 cut(s) 417, 749, 878, 879
AflIII ACRYGT 1 cut(s) 1131
AgsI TTSAA 9 cut(s) 147, 359, 506, 575, 829, 914, 1008, 1066, 1091
AjnI CCWGG 4 cut(s) 700, 742, 768, 979
AluBI AGCT 7 cut(s) 10, 32, 155, 270, 354, 1046, 1110
AluI AGCT 7 cut(s) 10, 32, 155, 270, 354, 1046, 1110
Alw21I GWGCWC 1 cut(s) 394
Alw26I GTCTC 2 cut(s) 694, 851
AoxI GGCC 4 cut(s) 331, 697, 771, 1126
ApeKI GCWGC 1 cut(s) 555
ApoI RAATTY 1 cut(s) 1038
Asp718I GGTACC 1 cut(s) 833
AspLEI GCGC 1 cut(s) 242
AspS9I GGNCC 3 cut(s) 698, 740, 997
AsuHPI GGTGA 2 cut(s) 89, 303
AvaII GGWCC 2 cut(s) 740, 997
BalI TGGCCA 2 cut(s) 773, 1128
BanI GGYRCC 2 cut(s) 704, 833
BauI CACGAG 2 cut(s) 302, 715
BbrPI CACGTG 1 cut(s) 1132
BbsI GAAGAC 1 cut(s) 982
Bbv12I GWGCWC 1 cut(s) 394
BbvI GCAGC 1 cut(s) 542
BccI CCATC 6 cut(s) 287, 452, 611, 674, 715, 1118
BceAI ACGGC 1 cut(s) 568
BciT130I CCWGG 4 cut(s) 702, 744, 770, 981
BciVI GTATCC 1 cut(s) 632
BcoDI GTCTC 2 cut(s) 694, 851
BfaI CTAG 1 cut(s) 329
BfmI CTRYAG 1 cut(s) 243
BfoI RGCGCY 1 cut(s) 243
BfuI GTATCC 1 cut(s) 632
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 2 cut(s) 556, 594
BlsI GCNGC 2 cut(s) 557, 595
BmcAI AGTACT 1 cut(s) 327
Bme1390I CCNGG 4 cut(s) 702, 744, 770, 981
Bme18I GGWCC 2 cut(s) 740, 997
BmgT120I GGNCC 3 cut(s) 698, 740, 997
BmiI GGNNCC 7 cut(s) 121, 187, 409, 463, 706, 767, 835
BmrFI CCNGG 4 cut(s) 702, 744, 770, 981
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 1 cut(s) 388
BmuI ACTGGG 1 cut(s) 684
BoxI GACNNNNGTC 1 cut(s) 514
BpiI GAAGAC 1 cut(s) 982
BsaAI YACGTR 1 cut(s) 1132
BsaHI GRCGYC 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 4 cut(s) 489, 700, 708, 743
Bsc4I CCNNNNNNNGG 4 cut(s) 417, 749, 878, 879
Bse1I ACTGG 4 cut(s) 350, 447, 631, 679
Bse3DI GCAATG 2 cut(s) 136, 588
BseBI CCWGG 4 cut(s) 702, 744, 770, 981
BseDI CCNNGG 4 cut(s) 489, 700, 708, 743
BseGI GGATG 1 cut(s) 726
BseLI CCNNNNNNNGG 4 cut(s) 417, 749, 878, 879
BseMI GCAATG 2 cut(s) 136, 588
BseMII CTCAG 4 cut(s) 195, 862, 992, 1041
BseNI ACTGG 4 cut(s) 350, 447, 631, 679
BseRI GAGGAG 1 cut(s) 402
BseXI GCAGC 1 cut(s) 542
BseYI CCCAGC 1 cut(s) 872
BshFI GGCC 4 cut(s) 333, 699, 773, 1128
BshNI GGYRCC 2 cut(s) 704, 833
BsiHKAI GWGCWC 1 cut(s) 394
BslFI GGGAC 1 cut(s) 692
BslI CCNNNNNNNGG 4 cut(s) 417, 749, 878, 879
BsmAI GTCTC 2 cut(s) 694, 851
BsmFI GGGAC 1 cut(s) 692
BsnI GGCC 4 cut(s) 333, 699, 773, 1128
Bso31I GGTCTC 1 cut(s) 851
Bsp1286I GDGCHC 1 cut(s) 394
BspACI CCGC 5 cut(s) 192, 369, 411, 594, 738
BspANI GGCC 4 cut(s) 333, 699, 773, 1128
BspCNI CTCAG 4 cut(s) 196, 861, 993, 1040
BspHI TCATGA 1 cut(s) 1136
BspLI GGNNCC 7 cut(s) 121, 187, 409, 463, 706, 767, 835
BspT107I GGYRCC 2 cut(s) 704, 833
BspTNI GGTCTC 1 cut(s) 851
BsrDI GCAATG 2 cut(s) 136, 588
BsrI ACTGG 4 cut(s) 350, 447, 631, 679
BssECI CCNNGG 4 cut(s) 489, 700, 708, 743
BssNI GRCGYC 1 cut(s) 579
BssSI CACGAG 2 cut(s) 302, 715
BssT1I CCWWGG 2 cut(s) 489, 708
Bst2BI CACGAG 2 cut(s) 302, 715
Bst2UI CCWGG 4 cut(s) 702, 744, 770, 981
Bst4CI ACNGT 2 cut(s) 959, 977
Bst6I CTCTTC 1 cut(s) 780
BstACI GRCGYC 1 cut(s) 579
BstAPI GCANNNNNTGC 2 cut(s) 398, 735
BstBAI YACGTR 1 cut(s) 1132
BstC8I GCNNGC 1 cut(s) 424
BstDEI CTNAG 5 cut(s) 6, 204, 848, 1001, 1027
BstF5I GGATG 1 cut(s) 726
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstMAI GTCTC 2 cut(s) 694, 851
BstMWI GCNNNNNNNGC 6 cut(s) 398, 419, 735, 765, 1116, 1125
BstNI CCWGG 4 cut(s) 702, 744, 770, 981
BstPAI GACNNNNGTC 1 cut(s) 514
BstSCI CCNGG 4 cut(s) 700, 742, 768, 979
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 1 cut(s) 542
BstV2I GAAGAC 1 cut(s) 982
BstXI CCANNNNNNTGG 1 cut(s) 781
BsuI GTATCC 1 cut(s) 632
BsuRI GGCC 4 cut(s) 333, 699, 773, 1128
BtsCI GGATG 1 cut(s) 726
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 4 cut(s) 201, 518, 612, 1071
Cac8I GCNNGC 1 cut(s) 424
CciI TCATGA 1 cut(s) 1136
CfoI GCGC 1 cut(s) 242
Cfr13I GGNCC 3 cut(s) 698, 740, 997
CseI GACGC 1 cut(s) 568
Csp6I GTAC 5 cut(s) 215, 326, 435, 566, 834
CviAII CATG 6 cut(s) 335, 626, 735, 749, 994, 1137
CviQI GTAC 5 cut(s) 215, 326, 435, 566, 834
DdeI CTNAG 5 cut(s) 6, 204, 848, 1001, 1027
DraI TTTAAA 1 cut(s) 253
DraIII CACNNNGTG 1 cut(s) 544
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 2 cut(s) 771, 1126
Eam1104I CTCTTC 1 cut(s) 780
EarI CTCTTC 1 cut(s) 780
EciI GGCGGA 1 cut(s) 400
Eco130I CCWWGG 2 cut(s) 489, 708
Eco31I GGTCTC 1 cut(s) 851
Eco47I GGWCC 2 cut(s) 740, 997
Eco72I CACGTG 1 cut(s) 1132
EcoRII CCWGG 4 cut(s) 700, 742, 768, 979
EcoT14I CCWWGG 2 cut(s) 489, 708
ErhI CCWWGG 2 cut(s) 489, 708
FaeI CATG 6 cut(s) 338, 629, 738, 752, 997, 1140
FaqI GGGAC 1 cut(s) 692
FatI CATG 6 cut(s) 334, 625, 734, 748, 993, 1136
FauI CCCGC 1 cut(s) 362
Fnu4HI GCNGC 2 cut(s) 556, 594
FokI GGATG 1 cut(s) 733
Fsp4HI GCNGC 2 cut(s) 556, 594
FspBI CTAG 1 cut(s) 329
GlaI GCGC 1 cut(s) 241
GluI GCNGC 2 cut(s) 556, 594
GsaI CCCAGC 1 cut(s) 876
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 4 cut(s) 333, 699, 773, 1128
HgaI GACGC 1 cut(s) 568
HhaI GCGC 1 cut(s) 242
Hin1I GRCGYC 1 cut(s) 579
Hin1II CATG 6 cut(s) 338, 629, 738, 752, 997, 1140
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HindIII AAGCTT 3 cut(s) 8, 153, 1108
HinfI GANTC 5 cut(s) 197, 571, 713, 795, 900
HphI GGTGA 2 cut(s) 89, 303
Hpy166II GTNNAC 1 cut(s) 962
Hpy188I TCNGA 5 cut(s) 112, 288, 386, 899, 1030
Hpy188III TCNNGA 5 cut(s) 455, 639, 850, 1000, 1137
Hpy8I GTNNAC 1 cut(s) 962
HpyAV CCTTC 3 cut(s) 353, 500, 785
HpyCH4III ACNGT 2 cut(s) 959, 977
HpyCH4IV ACGT 2 cut(s) 1061, 1131
HpyCH4V TGCA 6 cut(s) 129, 264, 426, 650, 889, 1022
HpyF10VI GCNNNNNNNGC 6 cut(s) 398, 419, 735, 765, 1116, 1125
HpyF3I CTNAG 5 cut(s) 6, 204, 848, 1001, 1027
HpySE526I ACGT 2 cut(s) 1061, 1131
Hsp92I GRCGYC 1 cut(s) 579
Hsp92II CATG 6 cut(s) 338, 629, 738, 752, 997, 1140
HspAI GCGC 1 cut(s) 240
KpnI GGTACC 1 cut(s) 837
LmnI GCTCC 4 cut(s) 119, 389, 461, 765
Lsp1109I GCAGC 1 cut(s) 542
LweI GCATC 1 cut(s) 388
MaeI CTAG 1 cut(s) 329
MaeII ACGT 2 cut(s) 1061, 1131
MaeIII GTNAC 4 cut(s) 136, 516, 805, 1069
MboII GAAGA 3 cut(s) 646, 797, 982
MhlI GDGCHC 1 cut(s) 394
MlsI TGGCCA 2 cut(s) 773, 1128
MluCI AATT 7 cut(s) 142, 280, 428, 942, 952, 1038, 1086
MluNI TGGCCA 2 cut(s) 773, 1128
MlyI GAGTC 2 cut(s) 707, 804
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 7 cut(s) 199, 219, 380, 673, 781, 980, 1048
Mox20I TGGCCA 2 cut(s) 773, 1128
MscI TGGCCA 2 cut(s) 773, 1128
MseI TTAA 4 cut(s) 252, 431, 921, 1115
Msp20I TGGCCA 2 cut(s) 773, 1128
MspA1I CMGCKG 3 cut(s) 32, 596, 1046
MspR9I CCNGG 4 cut(s) 702, 744, 770, 981
MvaI CCWGG 4 cut(s) 702, 744, 770, 981
MwoI GCNNNNNNNGC 6 cut(s) 398, 419, 735, 765, 1116, 1125
NlaIII CATG 6 cut(s) 338, 629, 738, 752, 997, 1140
NlaIV GGNNCC 7 cut(s) 121, 187, 409, 463, 706, 767, 835
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 516, 1069
PagI TCATGA 1 cut(s) 1136
PfeI GAWTC 3 cut(s) 197, 571, 900
PflFI GACNNNGTC 1 cut(s) 856
PkrI GCNGC 2 cut(s) 557, 595
PleI GAGTC 2 cut(s) 707, 803
PmaCI CACGTG 1 cut(s) 1132
PmlI CACGTG 1 cut(s) 1132
PpsI GAGTC 2 cut(s) 707, 803
Ppu21I YACGTR 1 cut(s) 1132
PshAI GACNNNNGTC 1 cut(s) 514
PsiI TTATAA 1 cut(s) 102
Psp1406I AACGTT 1 cut(s) 1061
Psp6I CCWGG 4 cut(s) 700, 742, 768, 979
PspCI CACGTG 1 cut(s) 1132
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 4 cut(s) 700, 742, 768, 979
PspN4I GGNNCC 7 cut(s) 121, 187, 409, 463, 706, 767, 835
PspPI GGNCC 3 cut(s) 698, 740, 997
PsyI GACNNNGTC 1 cut(s) 856
PvuII CAGCTG 2 cut(s) 32, 1046
RsaI GTAC 5 cut(s) 216, 327, 436, 567, 835
RsaNI GTAC 5 cut(s) 215, 326, 435, 566, 834
SaqAI TTAA 4 cut(s) 252, 431, 921, 1115
SatI GCNGC 2 cut(s) 556, 594
Sau96I GGNCC 3 cut(s) 698, 740, 997
ScaI AGTACT 1 cut(s) 327
SchI GAGTC 2 cut(s) 707, 804
ScrFI CCNGG 4 cut(s) 702, 744, 770, 981
SduI GDGCHC 1 cut(s) 394
SfaNI GCATC 1 cut(s) 388
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 2 cut(s) 740, 997
Sse9I AATT 7 cut(s) 142, 280, 428, 942, 952, 1038, 1086
SsiI CCGC 5 cut(s) 192, 369, 411, 594, 738
SspI AATATT 1 cut(s) 1094
SspMI CTAG 1 cut(s) 329
StyD4I CCNGG 4 cut(s) 700, 742, 768, 979
StyI CCWWGG 2 cut(s) 489, 708
TaaI ACNGT 2 cut(s) 959, 977
TaiI ACGT 2 cut(s) 1064, 1134
TaqI TCGA 1 cut(s) 657
TasI AATT 7 cut(s) 142, 280, 428, 942, 952, 1038, 1086
TatI WGTACW 2 cut(s) 325, 565
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 3 cut(s) 197, 571, 900
Tru1I TTAA 4 cut(s) 252, 431, 921, 1115
Tru9I TTAA 4 cut(s) 252, 431, 921, 1115
TscAI CASTG 4 cut(s) 208, 525, 619, 1078
TseFI GTSAC 2 cut(s) 516, 1069
TseI GCWGC 1 cut(s) 555
Tsp45I GTSAC 2 cut(s) 516, 1069
TspDTI ATGAA 2 cut(s) 28, 982
TspRI CASTG 4 cut(s) 208, 525, 619, 1078
Tth111I GACNNNGTC 1 cut(s) 856
VpaK11BI GGWCC 2 cut(s) 740, 997
XapI RAATTY 1 cut(s) 1038
XspI CTAG 1 cut(s) 329
ZrmI AGTACT 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.