Rroxscaffold_2G00126990

Polygalacturonase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001539
Physical Location & Seq
Forward (+)
62406481 .. 62411055
4575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00126990.1

Sequence Viewer

Length: 489 bp
ATGGGCATCCATGTCCAGTTTTCCAGAAATGTTGCAATCTTCGACACCTCCATCAAAACTGAGGACGATTGTGTCTCTATTGGCCCTGGCACCAAGGAATTGTGGATGGAACGCATATCATGCGGACCTGGCCATGGCATTAGCATAGGGAGCCTGGCCAAAGACTTGGAAGAGGAAGGAGTCCAAAATGTAACACTGAAAAATGCAATTTTCAAGGATACTACAAATGGTCTCAGGATAAAGTCCTGGGCAAGACCCAGCACCGGGTTTGTGCAAGGTGTCCAATCCCTTGATGTTGAAATGATTAATGTCCAAAACCCCATTGTAATTGACCAGAATTACTGCCCACACAACCTCAACTGTCCTGGTCAGGTCTTCATGGTCCTTAGTTCAAAGAACATTTGGCAAACTCAGATTGGAAAATTAGATGTGTTTGAGGGAACTTTGAGAGTCAATGGTTCTGGTAATATGAATATTACTCTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

162

Amino Acids

17.8

Weight (kDa)

5.75

Isoelectric Point (pI)

26.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 2 - 119 1.3e-38 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
AccB1I GGYRCC 1 cut(s) 89
AciI CCGC 1 cut(s) 123
AcoI YGGCCR 2 cut(s) 130, 156
AfiI CCNNNNNNNGG 3 cut(s) 134, 263, 264
AgsI TTSAA 3 cut(s) 214, 299, 393
AjnI CCWGG 5 cut(s) 85, 127, 153, 245, 364
Alw26I GTCTC 2 cut(s) 79, 236
AoxI GGCC 3 cut(s) 82, 130, 156
AseI ATTAAT 1 cut(s) 306
AspS9I GGNCC 3 cut(s) 83, 125, 382
AsuC2I CCSGG 1 cut(s) 265
AvaII GGWCC 2 cut(s) 125, 382
BalI TGGCCA 2 cut(s) 132, 158
BanI GGYRCC 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 367
BccI CCATC 2 cut(s) 59, 100
BciT130I CCWGG 5 cut(s) 87, 129, 155, 247, 366
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 1 cut(s) 265
BcoDI GTCTC 2 cut(s) 79, 236
BfuI GTATCC 1 cut(s) 211
Bme1390I CCNGG 6 cut(s) 87, 129, 155, 247, 265, 366
Bme18I GGWCC 2 cut(s) 125, 382
BmgT120I GGNCC 3 cut(s) 83, 125, 382
BmiI GGNNCC 2 cut(s) 91, 152
BmrFI CCNGG 6 cut(s) 87, 129, 155, 247, 265, 366
BmsI GCATC 1 cut(s) 15
BpiI GAAGAC 1 cut(s) 367
BpuMI CCSGG 1 cut(s) 265
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 4 cut(s) 85, 93, 133, 246
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 263, 264
Bse1I ACTGG 1 cut(s) 16
BseBI CCWGG 5 cut(s) 87, 129, 155, 247, 366
BseDI CCNNGG 4 cut(s) 85, 93, 133, 246
BseGI GGATG 2 cut(s) 6, 111
BseLI CCNNNNNNNGG 3 cut(s) 134, 263, 264
BseMII CTCAG 3 cut(s) 51, 247, 425
BseNI ACTGG 1 cut(s) 16
BseYI CCCAGC 1 cut(s) 257
BshFI GGCC 3 cut(s) 84, 132, 158
BshNI GGYRCC 1 cut(s) 89
BsiSI CCGG 1 cut(s) 264
BslI CCNNNNNNNGG 3 cut(s) 134, 263, 264
BsmAI GTCTC 2 cut(s) 79, 236
BsnI GGCC 3 cut(s) 84, 132, 158
Bso31I GGTCTC 1 cut(s) 236
Bsp19I CCATGG 1 cut(s) 133
BspACI CCGC 1 cut(s) 123
BspANI GGCC 3 cut(s) 84, 132, 158
BspCNI CTCAG 3 cut(s) 52, 246, 424
BspLI GGNNCC 2 cut(s) 91, 152
BspT107I GGYRCC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 236
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 4 cut(s) 85, 93, 133, 246
BssT1I CCWWGG 2 cut(s) 93, 133
Bst2UI CCWGG 5 cut(s) 87, 129, 155, 247, 366
Bst4CI ACNGT 1 cut(s) 362
Bst6I CTCTTC 2 cut(s) 165, 486
BstAPI GCANNNNNTGC 1 cut(s) 120
BstDEI CTNAG 4 cut(s) 60, 233, 386, 411
BstDSI CCRYGG 1 cut(s) 133
BstF5I GGATG 2 cut(s) 6, 111
BstMAI GTCTC 2 cut(s) 79, 236
BstMWI GCNNNNNNNGC 3 cut(s) 120, 129, 150
BstNI CCWGG 5 cut(s) 87, 129, 155, 247, 366
BstSCI CCNGG 6 cut(s) 85, 127, 153, 245, 263, 364
BstV2I GAAGAC 1 cut(s) 367
BstXI CCANNNNNNTGG 1 cut(s) 166
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 3 cut(s) 84, 132, 158
BtgI CCRYGG 1 cut(s) 133
BtsCI GGATG 2 cut(s) 6, 111
BtsIMutI CAGTG 1 cut(s) 194
Cfr13I GGNCC 3 cut(s) 83, 125, 382
CviAII CATG 4 cut(s) 11, 120, 134, 379
CviJI RGCY 4 cut(s) 84, 132, 153, 158
CviKI_1 RGCY 4 cut(s) 84, 132, 153, 158
DdeI CTNAG 4 cut(s) 60, 233, 386, 411
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 2 cut(s) 130, 156
Eam1104I CTCTTC 2 cut(s) 165, 486
EarI CTCTTC 2 cut(s) 165, 486
Eco130I CCWWGG 2 cut(s) 93, 133
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 125, 382
EcoRII CCWGG 5 cut(s) 85, 127, 153, 245, 364
EcoT14I CCWWGG 2 cut(s) 93, 133
ErhI CCWWGG 2 cut(s) 93, 133
FaeI CATG 4 cut(s) 14, 123, 137, 382
FaiI YATR 7 cut(s) 12, 116, 121, 135, 146, 380, 470
FatI CATG 4 cut(s) 10, 119, 133, 378
FokI GGATG 1 cut(s) 118
GsaI CCCAGC 1 cut(s) 261
HaeIII GGCC 3 cut(s) 84, 132, 158
HapII CCGG 1 cut(s) 264
Hin1II CATG 4 cut(s) 14, 123, 137, 382
HinfI GANTC 2 cut(s) 180, 450
HpaII CCGG 1 cut(s) 264
Hpy188I TCNGA 1 cut(s) 414
Hpy188III TCNNGA 2 cut(s) 24, 235
HpyAV CCTTC 1 cut(s) 170
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4V TGCA 3 cut(s) 35, 206, 274
HpyF10VI GCNNNNNNNGC 3 cut(s) 120, 129, 150
HpyF3I CTNAG 4 cut(s) 60, 233, 386, 411
Hsp92II CATG 4 cut(s) 14, 123, 137, 382
LmnI GCTCC 1 cut(s) 150
LweI GCATC 1 cut(s) 15
MaeIII GTNAC 1 cut(s) 190
MboII GAAGA 4 cut(s) 31, 182, 367, 473
MlsI TGGCCA 2 cut(s) 132, 158
MluCI AATT 5 cut(s) 98, 207, 327, 337, 422
MluNI TGGCCA 2 cut(s) 132, 158
MlyI GAGTC 2 cut(s) 189, 459
MnlI CCTC 5 cut(s) 55, 58, 166, 365, 430
Mox20I TGGCCA 2 cut(s) 132, 158
MscI TGGCCA 2 cut(s) 132, 158
MseI TTAA 1 cut(s) 306
Msp20I TGGCCA 2 cut(s) 132, 158
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 6 cut(s) 87, 129, 155, 247, 265, 366
MvaI CCWGG 5 cut(s) 87, 129, 155, 247, 366
MwoI GCNNNNNNNGC 3 cut(s) 120, 129, 150
NciI CCSGG 1 cut(s) 265
NcoI CCATGG 1 cut(s) 133
NlaIII CATG 4 cut(s) 14, 123, 137, 382
NlaIV GGNNCC 2 cut(s) 91, 152
PleI GAGTC 2 cut(s) 188, 458
PpsI GAGTC 2 cut(s) 188, 458
PshBI ATTAAT 1 cut(s) 306
Psp6I CCWGG 5 cut(s) 85, 127, 153, 245, 364
PspFI CCCAGC 1 cut(s) 257
PspGI CCWGG 5 cut(s) 85, 127, 153, 245, 364
PspN4I GGNNCC 2 cut(s) 91, 152
PspPI GGNCC 3 cut(s) 83, 125, 382
SaqAI TTAA 1 cut(s) 306
Sau96I GGNCC 3 cut(s) 83, 125, 382
SchI GAGTC 2 cut(s) 189, 459
ScrFI CCNGG 6 cut(s) 87, 129, 155, 247, 265, 366
SetI ASST 5 cut(s) 50, 130, 280, 357, 375
SfaNI GCATC 1 cut(s) 15
SinI GGWCC 2 cut(s) 125, 382
Sse9I AATT 5 cut(s) 98, 207, 327, 337, 422
SsiI CCGC 1 cut(s) 123
SspI AATATT 1 cut(s) 475
StyD4I CCNGG 6 cut(s) 85, 127, 153, 245, 263, 364
StyI CCWWGG 2 cut(s) 93, 133
TaaI ACNGT 1 cut(s) 362
TaqI TCGA 1 cut(s) 42
TasI AATT 5 cut(s) 98, 207, 327, 337, 422
Tru1I TTAA 1 cut(s) 306
Tru9I TTAA 1 cut(s) 306
TscAI CASTG 1 cut(s) 201
TspDTI ATGAA 3 cut(s) 367, 473, 485
TspRI CASTG 1 cut(s) 201
VpaK11BI GGWCC 2 cut(s) 125, 382
VspI ATTAAT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.