FvH4_2g00671

No apical meristem-associated C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001696
Physical Location & Seq
Forward (+)
708938 .. 709768
831 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00671.t1

Sequence Viewer

Length: 744 bp
ATGGCAGATGTTAGGCAAAAGAAAGTTGTCCAACGAAAAGGAAATTTTTCGATCCAAGAAGATGAACTCTTATGTCATATATATCTTGAAATTTCTCAAGATCCTATCGATGGTGTTAACCAAAAGAAGGATAAAGTATGGGCGAGGATCACTGAATTGTGGAATCAAGATAAGGATGAGAATCAGACGAGAACATCCAAGTCTCTTCAAAGTCGATTTGTTGATCTTACTTACTGTGTAAGCAAATTTCGTGGTGCTGTTCGTCAAGTGGAAGATGACAATCCAAGCAGTGCAAATGAGAAAGATATTTGTGCCAAAGCAAAACAAATTCTACTAGATGATCCTAACTTCACAAAGAAGAAACTTCAGTTTGATCATGTGTGGCATATTCTTAAGGATGCTGAAAAGTGGGCCGATACAGGTAGAAGTTCAACAAGACCACGACAAAGAAATGATTCAAGTGAAGGATATGAATCAGCATCTCGCACATCACAATTGTCAGGTACACTACCATTTGATGTCGATTTAAATGCAGATGAAGACGAACAAGAGCTATATAATGATGAACTCGGCACGTCTCGTCGACCCATTGGTGTAAAGAAAGCAAAGCTGAAAAGGAAAGAGGCCAATGAGCAGACCAAGATTGCTAAACAAAACAGAGAATCCAATCAAGAATTGAAGGAGTTATTTGAAAAAAGTCTGCTAGAGAGAAATGCTTTTACTGTTAAGCACGAGCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM-associated

Protein Analysis

248

Amino Acids

28.72

Weight (kDa)

6.93

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM-associated PF14303 120 - 229 1.8e-11 No apical meristem-associated C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 583
AclWI GGATC 4 cut(s) 46, 95, 155, 335
AcsI RAATTY 4 cut(s) 43, 90, 245, 327
AcuI CTGAAG 1 cut(s) 350
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 2 cut(s) 110, 127
AflII CTTAAG 1 cut(s) 392
AgsI TTSAA 6 cut(s) 89, 209, 432, 459, 679, 692
AjiI CACGTC 1 cut(s) 576
AluBI AGCT 2 cut(s) 553, 610
AluI AGCT 2 cut(s) 553, 610
Alw26I GTCTC 2 cut(s) 207, 582
AlwI GGATC 4 cut(s) 46, 95, 155, 335
AoxI GGCC 2 cut(s) 411, 624
ApoI RAATTY 4 cut(s) 43, 90, 245, 327
Asp700I GAANNNNTTC 2 cut(s) 46, 454
AspS9I GGNCC 1 cut(s) 411
BauI CACGAG 1 cut(s) 731
BbsI GAAGAC 1 cut(s) 546
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 2 cut(s) 207, 582
BfaI CTAG 2 cut(s) 335, 704
BfrI CTTAAG 1 cut(s) 392
BmgBI CACGTC 1 cut(s) 576
BmgT120I GGNCC 1 cut(s) 411
BmsI GCATC 2 cut(s) 388, 488
BpiI GAAGAC 1 cut(s) 546
BpuEI CTTGAG 1 cut(s) 81
Bsa29I ATCGAT 1 cut(s) 108
BsaBI GATNNNNATC 3 cut(s) 180, 279, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 127
Bse8I GATNNNNATC 3 cut(s) 180, 279, 472
BseCI ATCGAT 1 cut(s) 108
BseGI GGATG 3 cut(s) 181, 194, 403
BseJI GATNNNNATC 3 cut(s) 180, 279, 472
BseLI CCNNNNNNNGG 2 cut(s) 110, 127
BshFI GGCC 2 cut(s) 413, 626
BshVI ATCGAT 1 cut(s) 108
BslI CCNNNNNNNGG 2 cut(s) 110, 127
BsmAI GTCTC 2 cut(s) 207, 582
BsmBI CGTCTC 1 cut(s) 582
BsnI GGCC 2 cut(s) 413, 626
Bsp143I GATC 6 cut(s) 51, 100, 147, 223, 340, 373
BspANI GGCC 2 cut(s) 413, 626
BspDI ATCGAT 1 cut(s) 108
BspPI GGATC 4 cut(s) 46, 95, 155, 335
BspTI CTTAAG 1 cut(s) 392
BssMI GATC 6 cut(s) 51, 100, 147, 223, 340, 373
BssSI CACGAG 1 cut(s) 731
Bst2BI CACGAG 1 cut(s) 731
Bst4CI ACNGT 2 cut(s) 236, 724
Bst6I CTCTTC 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 392
BstF5I GGATG 3 cut(s) 181, 194, 403
BstKTI GATC 6 cut(s) 54, 103, 150, 226, 343, 376
BstMAI GTCTC 2 cut(s) 207, 582
BstMBI GATC 6 cut(s) 51, 100, 147, 223, 340, 373
BstV2I GAAGAC 1 cut(s) 546
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 2 cut(s) 413, 626
BsuTUI ATCGAT 1 cut(s) 108
BtrI CACGTC 1 cut(s) 576
BtsCI GGATG 3 cut(s) 181, 194, 403
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 2 cut(s) 150, 295
Cfr13I GGNCC 1 cut(s) 411
ClaI ATCGAT 1 cut(s) 108
Csp6I GTAC 1 cut(s) 504
CspCI CAANNNNNGTGG 2 cut(s) 232, 267
CviAII CATG 1 cut(s) 377
CviJI RGCY 4 cut(s) 413, 553, 610, 626
CviKI_1 RGCY 4 cut(s) 413, 553, 610, 626
CviQI GTAC 1 cut(s) 504
DpnI GATC 6 cut(s) 53, 102, 149, 225, 342, 375
DpnII GATC 6 cut(s) 51, 100, 147, 223, 340, 373
DraI TTTAAA 1 cut(s) 528
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Eco57I CTGAAG 1 cut(s) 350
Esp3I CGTCTC 1 cut(s) 582
FaeI CATG 1 cut(s) 380
FatI CATG 1 cut(s) 376
FbaI TGATCA 1 cut(s) 373
FblI GTMKAC 1 cut(s) 583
FokI GGATG 3 cut(s) 181, 188, 410
FspBI CTAG 2 cut(s) 335, 704
HaeIII GGCC 2 cut(s) 413, 626
Hin1II CATG 1 cut(s) 380
HincII GTYRAC 2 cut(s) 118, 584
HindII GTYRAC 2 cut(s) 118, 584
HinfI GANTC 5 cut(s) 163, 181, 455, 473, 662
HpaI GTTAAC 1 cut(s) 118
Hpy166II GTNNAC 3 cut(s) 118, 506, 584
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 4 cut(s) 86, 98, 167, 671
Hpy8I GTNNAC 3 cut(s) 118, 506, 584
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 3 cut(s) 121, 458, 673
HpyCH4III ACNGT 2 cut(s) 236, 724
HpyCH4IV ACGT 1 cut(s) 575
HpyCH4V TGCA 2 cut(s) 293, 533
HpySE526I ACGT 1 cut(s) 575
Hsp92II CATG 1 cut(s) 380
Ksp22I TGATCA 1 cut(s) 373
KspAI GTTAAC 1 cut(s) 118
Kzo9I GATC 6 cut(s) 51, 100, 147, 223, 340, 373
LpnPI CCDG 2 cut(s) 405, 486
LweI GCATC 2 cut(s) 388, 488
MaeI CTAG 2 cut(s) 335, 704
MaeII ACGT 1 cut(s) 575
MalI GATC 6 cut(s) 53, 102, 149, 225, 342, 375
MboI GATC 6 cut(s) 51, 100, 147, 223, 340, 373
MboII GAAGA 5 cut(s) 71, 197, 284, 370, 551
MfeI CAATTG 1 cut(s) 494
MflI RGATCY 1 cut(s) 100
MluCI AATT 7 cut(s) 43, 90, 155, 245, 327, 494, 674
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 2 cut(s) 138, 616
MroXI GAANNNNTTC 2 cut(s) 46, 454
MseI TTAA 4 cut(s) 117, 393, 527, 726
MspCI CTTAAG 1 cut(s) 392
MunI CAATTG 1 cut(s) 494
NdeII GATC 6 cut(s) 51, 100, 147, 223, 340, 373
NlaIII CATG 1 cut(s) 380
NmeAIII GCCGAG 1 cut(s) 549
PdmI GAANNNNTTC 2 cut(s) 46, 454
PfeI GAWTC 5 cut(s) 163, 181, 455, 473, 662
PspPI GGNCC 1 cut(s) 411
PsuI RGATCY 1 cut(s) 100
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
SalI GTCGAC 1 cut(s) 582
SaqAI TTAA 4 cut(s) 117, 393, 527, 726
Sau3AI GATC 6 cut(s) 51, 100, 147, 223, 340, 373
Sau96I GGNCC 1 cut(s) 411
SetI ASST 5 cut(s) 424, 505, 555, 578, 612
SfaNI GCATC 2 cut(s) 388, 488
SmiI ATTTAAAT 1 cut(s) 528
SmlI CTYRAG 2 cut(s) 96, 392
SmoI CTYRAG 2 cut(s) 96, 392
Sse9I AATT 7 cut(s) 43, 90, 155, 245, 327, 494, 674
SspI AATATT 1 cut(s) 740
SspMI CTAG 2 cut(s) 335, 704
SwaI ATTTAAAT 1 cut(s) 528
TaaI ACNGT 2 cut(s) 236, 724
TaiI ACGT 1 cut(s) 578
TaqI TCGA 5 cut(s) 50, 108, 214, 522, 583
TasI AATT 7 cut(s) 43, 90, 155, 245, 327, 494, 674
TfiI GAWTC 5 cut(s) 163, 181, 455, 473, 662
Tru1I TTAA 4 cut(s) 117, 393, 527, 726
Tru9I TTAA 4 cut(s) 117, 393, 527, 726
TscAI CASTG 2 cut(s) 157, 295
TspDTI ATGAA 4 cut(s) 78, 486, 552, 579
TspRI CASTG 2 cut(s) 157, 295
Vha464I CTTAAG 1 cut(s) 392
XapI RAATTY 4 cut(s) 43, 90, 245, 327
XmiI GTMKAC 1 cut(s) 583
XmnI GAANNNNTTC 2 cut(s) 46, 454
XspI CTAG 2 cut(s) 335, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.