FvH4_2g13881

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000751
Physical Location & Seq
Reverse (-)
12165347 .. 12166212
866 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13881.t1

Sequence Viewer

Length: 699 bp
ATGTCTTGCTTTGAGGTTGAAGGAAATCGCGGGGGAACCATGACACCAATATCCGAGTGGAAGTTGGAGCAGCACAAGCAGTTCTTGGATACTTTCATACTCATAGGCGGTGATAAAGAACAGGTGGGAGTACACACTGCCTTAAAGGCGGAGCTACTGAGCCTGGCGGGGTGTTCTTGTAGAACCTTTTTTCCAGACTTCGACATTACCTCACTTGAAATACTACAGAGAAGATCCCAAGCACTGTTTTCCTCTGACGAAGTCACCCTTGACAGCCTATACGTGGCACCTAAAAAGATGTCATCGTCTTGGACGCTGGAGCATCACCGGCGAGTAGTGAGGTCGGTGATACAGCTGGGTGGGCTTAACAGTAGCAAGACTACCGCAGAGGCCGTGCTGAAGTTGATGGATGAGGAGGACCGCCGCCAAGGCCTCACCGCAAAGATGATAGAGCACCACCTTGTCGGGTTGTATGGTTTGCAGAAAGAAACTCAACGGCTAATGGCTTTTTGTGAAAAAGAAAAGGAGCAATCGTTTTGGGATCTCCGGTACCTTGTTCGTAAAGGCCTATATTACGTCCTGTTCACAAAGATAAAAGAGAATCCACATCTCCGACAGCATCTGAAGCTCCTCAAACAAGTAAAGAAAGAAACCCTCATCTGCGAATTATTGGATCTTTACGATGACCAAAAACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

233

Amino Acids

26.82

Weight (kDa)

7.67

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 549
AccB1I GGYRCC 2 cut(s) 286, 549
AccII CGCG 1 cut(s) 30
AciI CCGC 8 cut(s) 30, 108, 149, 167, 384, 421, 424, 438
AclWI GGATC 3 cut(s) 228, 549, 681
AcuI CTGAAG 2 cut(s) 419, 644
AfaI GTAC 2 cut(s) 132, 551
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 2 cut(s) 20, 218
AjnI CCWGG 1 cut(s) 162
AloI GAACNNNNNNTCC 2 cut(s) 175, 207
AluBI AGCT 3 cut(s) 154, 355, 628
AluI AGCT 3 cut(s) 154, 355, 628
Alw21I GWGCWC 1 cut(s) 456
AlwI GGATC 3 cut(s) 228, 549, 681
AlwNI CAGNNNCTG 1 cut(s) 622
AoxI GGCC 3 cut(s) 390, 430, 565
ApeKI GCWGC 1 cut(s) 70
Asp718I GGTACC 1 cut(s) 549
AspS9I GGNCC 1 cut(s) 418
AsuHPI GGTGA 5 cut(s) 122, 256, 317, 358, 427
AvaII GGWCC 1 cut(s) 418
BanI GGYRCC 2 cut(s) 286, 549
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 1 cut(s) 82
BccI CCATC 1 cut(s) 400
BceAI ACGGC 2 cut(s) 377, 512
BciT130I CCWGG 1 cut(s) 164
BciVI GTATCC 1 cut(s) 82
BfmI CTRYAG 1 cut(s) 224
BfuI GTATCC 1 cut(s) 82
BglI GCCNNNNNGGC 2 cut(s) 146, 429
BisI GCNGC 2 cut(s) 71, 424
BlsI GCNGC 2 cut(s) 72, 425
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 3 cut(s) 37, 288, 551
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 2 cut(s) 331, 628
BpmI CTGGAG 1 cut(s) 338
BsaAI YACGTR 1 cut(s) 283
BsaJI CCNNGG 1 cut(s) 427
BsaWI WCCGGW 1 cut(s) 546
BsaXI ACNNNNNCTCC 2 cut(s) 518, 548
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse118I RCCGGY 1 cut(s) 327
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 149
BseRI GAGGAG 2 cut(s) 428, 620
BseXI GCAGC 1 cut(s) 82
BseYI CCCAGC 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 30
BshFI GGCC 3 cut(s) 392, 432, 567
BshNI GGYRCC 2 cut(s) 286, 549
BsiHKAI GWGCWC 1 cut(s) 456
BsiSI CCGG 2 cut(s) 328, 547
BslI CCNNNNNNNGG 1 cut(s) 283
BsnI GGCC 3 cut(s) 392, 432, 567
Bsp1286I GDGCHC 1 cut(s) 456
Bsp143I GATC 3 cut(s) 233, 541, 673
BspACI CCGC 8 cut(s) 30, 108, 149, 167, 384, 421, 424, 438
BspANI GGCC 3 cut(s) 392, 432, 567
BspCNI CTCAG 1 cut(s) 150
BspFNI CGCG 1 cut(s) 30
BspLI GGNNCC 3 cut(s) 37, 288, 551
BspPI GGATC 3 cut(s) 228, 549, 681
BspT107I GGYRCC 2 cut(s) 286, 549
BsrFI RCCGGY 1 cut(s) 327
BssAI RCCGGY 1 cut(s) 327
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 3 cut(s) 233, 541, 673
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 246, 371
BstBAI YACGTR 1 cut(s) 283
BstDEI CTNAG 2 cut(s) 158, 696
BstF5I GGATG 1 cut(s) 415
BstFNI CGCG 1 cut(s) 30
BstKTI GATC 3 cut(s) 236, 544, 676
BstMBI GATC 3 cut(s) 233, 541, 673
BstMWI GCNNNNNNNGC 6 cut(s) 76, 146, 328, 361, 429, 625
BstNI CCWGG 1 cut(s) 164
BstSCI CCNGG 1 cut(s) 162
BstSFI CTRYAG 1 cut(s) 224
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 1 cut(s) 82
BstX2I RGATCY 3 cut(s) 233, 541, 673
BstYI RGATCY 3 cut(s) 233, 541, 673
BsuI GTATCC 1 cut(s) 82
BsuRI GGCC 3 cut(s) 392, 432, 567
BtsCI GGATG 1 cut(s) 415
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 135, 242
CaiI CAGNNNCTG 1 cut(s) 622
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 1 cut(s) 418
CseI GACGC 1 cut(s) 322
Csp6I GTAC 2 cut(s) 131, 550
CviAII CATG 1 cut(s) 40
CviQI GTAC 2 cut(s) 131, 550
DdeI CTNAG 2 cut(s) 158, 696
DpnI GATC 3 cut(s) 235, 543, 675
DpnII GATC 3 cut(s) 233, 541, 673
EciI GGCGGA 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 427
Eco147I AGGCCT 2 cut(s) 432, 567
Eco47I GGWCC 1 cut(s) 418
Eco57I CTGAAG 2 cut(s) 419, 644
EcoRII CCWGG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 1 cut(s) 43
FaiI YATR 6 cut(s) 41, 98, 104, 280, 474, 571
FalI AAGNNNNNCTT 4 cut(s) 68, 100, 252, 284
FatI CATG 1 cut(s) 39
FauI CCCGC 2 cut(s) 23, 160
Fnu4HI GCNGC 2 cut(s) 71, 424
FokI GGATG 1 cut(s) 422
Fsp4HI GCNGC 2 cut(s) 71, 424
GluI GCNGC 2 cut(s) 71, 424
GsaI CCCAGC 1 cut(s) 359
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 3 cut(s) 392, 432, 567
HapII CCGG 2 cut(s) 328, 547
HgaI GACGC 1 cut(s) 322
Hin1II CATG 1 cut(s) 43
HinfI GANTC 1 cut(s) 601
HpaII CCGG 2 cut(s) 328, 547
HphI GGTGA 5 cut(s) 122, 256, 317, 358, 427
Hpy166II GTNNAC 2 cut(s) 133, 585
Hpy188I TCNGA 4 cut(s) 55, 256, 614, 624
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 2 cut(s) 133, 585
HpyAV CCTTC 1 cut(s) 14
HpyCH4III ACNGT 2 cut(s) 246, 371
HpyCH4IV ACGT 2 cut(s) 282, 576
HpyCH4V TGCA 1 cut(s) 481
HpyF10VI GCNNNNNNNGC 6 cut(s) 76, 146, 328, 361, 429, 625
HpyF3I CTNAG 2 cut(s) 158, 696
HpySE526I ACGT 2 cut(s) 282, 576
Hsp92II CATG 1 cut(s) 43
KpnI GGTACC 1 cut(s) 553
Kzo9I GATC 3 cut(s) 233, 541, 673
LmnI GCTCC 5 cut(s) 67, 151, 319, 526, 633
LpnPI CCDG 9 cut(s) 107, 149, 176, 207, 302, 341, 341, 560, 593
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 2 cut(s) 331, 628
MaeII ACGT 2 cut(s) 282, 576
MaeIII GTNAC 1 cut(s) 262
MalI GATC 3 cut(s) 235, 543, 675
MboI GATC 3 cut(s) 233, 541, 673
MboII GAAGA 1 cut(s) 243
MflI RGATCY 3 cut(s) 233, 541, 673
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 1 cut(s) 665
MmeI TCCRAC 2 cut(s) 45, 637
MseI TTAA 2 cut(s) 143, 366
MspA1I CMGCKG 1 cut(s) 355
MspI CCGG 2 cut(s) 328, 547
MspR9I CCNGG 1 cut(s) 164
MvaI CCWGG 1 cut(s) 164
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 6 cut(s) 76, 146, 328, 361, 429, 625
NdeII GATC 3 cut(s) 233, 541, 673
NlaIII CATG 1 cut(s) 43
NlaIV GGNNCC 3 cut(s) 37, 288, 551
NmuCI GTSAC 1 cut(s) 262
PceI AGGCCT 2 cut(s) 432, 567
PfeI GAWTC 1 cut(s) 601
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 2 cut(s) 72, 425
Ppu21I YACGTR 1 cut(s) 283
Psp6I CCWGG 1 cut(s) 162
PspFI CCCAGC 1 cut(s) 355
PspGI CCWGG 1 cut(s) 162
PspN4I GGNNCC 3 cut(s) 37, 288, 551
PspPI GGNCC 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 622
PsuI RGATCY 3 cut(s) 233, 541, 673
PsyI GACNNNGTC 1 cut(s) 260
PvuII CAGCTG 1 cut(s) 355
RsaI GTAC 2 cut(s) 132, 551
RsaNI GTAC 2 cut(s) 131, 550
SaqAI TTAA 2 cut(s) 143, 366
SatI GCNGC 2 cut(s) 71, 424
Sau3AI GATC 3 cut(s) 233, 541, 673
Sau96I GGNCC 1 cut(s) 418
ScrFI CCNGG 1 cut(s) 164
SduI GDGCHC 1 cut(s) 456
SfaNI GCATC 2 cut(s) 331, 628
SfcI CTRYAG 1 cut(s) 224
SgrAI CRCCGGYG 1 cut(s) 327
SinI GGWCC 1 cut(s) 418
Sse9I AATT 1 cut(s) 665
SseBI AGGCCT 2 cut(s) 432, 567
SsiI CCGC 8 cut(s) 30, 108, 149, 167, 384, 421, 424, 438
StuI AGGCCT 2 cut(s) 432, 567
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 2 cut(s) 246, 371
TaiI ACGT 2 cut(s) 285, 579
TaqI TCGA 1 cut(s) 201
TasI AATT 1 cut(s) 665
TatI WGTACW 1 cut(s) 130
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 1 cut(s) 601
Tru1I TTAA 2 cut(s) 143, 366
Tru9I TTAA 2 cut(s) 143, 366
TscAI CASTG 2 cut(s) 142, 249
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 1 cut(s) 70
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 85
TspRI CASTG 2 cut(s) 142, 249
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 1 cut(s) 418
XcmI CCANNNNNNNNNTGG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.