Rmu_sc0000271.1_g000010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000271.1
OG0000751
Physical Location & Seq
Forward (+)
50837 .. 51388
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000271.1_g000010.1.cds

Sequence Viewer

Length: 552 bp
atgttcgagtggacgttggagcaccacaagcaatgcatggatacttttatcctcttgggtggtgataaagaaacagttggagtacacaatgccttaaaggcggagctattgagcctggcagggtgttgttgtagaagcttttaccttgacttccacatttccttgcttagggtactacaaaaaaggtctgaggcattattttccagtaacaaagtcacccttgaaagactatacgtggcacctaaaaagatgtcattgtcatggatgttggaacatcaacaacaggttgtggaagcggtgatacaactcggtgggcttagcaatggcaagactaccccaaagtttgtgctgaagttgatagatgaggaagaccactgccaaggcctaaccgcaaagatgatagtgcaccaccttgtagggttgtatcatttgcaaaaggaaactcaacgactattggcttttggaatggtcggtggtgaaaaagaaacagagctgtcggttagggagctccagtaccttgtttttaaaaggattgccccaacaacgagataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

183

Amino Acids

20.88

Weight (kDa)

8.26

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AciI CCGC 3 cut(s) 101, 296, 390
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 3 cut(s) 84, 174, 515
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 1 cut(s) 224
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 4 cut(s) 106, 138, 493, 508
AluI AGCT 4 cut(s) 106, 138, 493, 508
Alw21I GWGCWC 3 cut(s) 24, 408, 510
Alw44I GTGCAC 1 cut(s) 404
AoxI GGCC 1 cut(s) 382
ApaLI GTGCAC 1 cut(s) 404
AsuHPI GGTGA 4 cut(s) 74, 208, 310, 488
BaeGI GKGCMC 1 cut(s) 408
BanI GGYRCC 1 cut(s) 238
BanII GRGCYC 1 cut(s) 510
BarI GAAGNNNNNNTAC 2 cut(s) 285, 317
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 3 cut(s) 24, 408, 510
BciT130I CCWGG 1 cut(s) 116
BciVI GTATCC 1 cut(s) 34
BfuI GTATCC 1 cut(s) 34
BglI GCCNNNNNGGC 1 cut(s) 98
BlpI GCTNAGC 1 cut(s) 317
Bme1390I CCNGG 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 167
Bpu1102I GCTNAGC 1 cut(s) 317
BsaAI YACGTR 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 379
BsaXI ACNNNNNCTCC 2 cut(s) 497, 527
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse1I ACTGG 2 cut(s) 204, 511
Bse3DI GCAATG 2 cut(s) 38, 328
BseBI CCWGG 1 cut(s) 116
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMI GCAATG 2 cut(s) 38, 328
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 204, 511
BseSI GKGCMC 1 cut(s) 408
BshFI GGCC 1 cut(s) 384
BshNI GGYRCC 1 cut(s) 238
BsiHKAI GWGCWC 3 cut(s) 24, 408, 510
BslI CCNNNNNNNGG 1 cut(s) 168
BsnI GGCC 1 cut(s) 384
Bsp1286I GDGCHC 3 cut(s) 24, 408, 510
Bsp1720I GCTNAGC 1 cut(s) 317
BspACI CCGC 3 cut(s) 101, 296, 390
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 238
BsrDI GCAATG 2 cut(s) 38, 328
BsrI ACTGG 2 cut(s) 204, 511
BssECI CCNNGG 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 379
Bst2UI CCWGG 1 cut(s) 116
Bst4CI ACNGT 1 cut(s) 76
BstBAI YACGTR 1 cut(s) 235
BstDEI CTNAG 3 cut(s) 167, 189, 317
BstENI CCTNNNNNAGG 1 cut(s) 166
BstF5I GGATG 1 cut(s) 270
BstMWI GCNNNNNNNGC 2 cut(s) 28, 98
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstSLI GKGCMC 1 cut(s) 408
BstV2I GAAGAC 1 cut(s) 375
BsuI GTATCC 1 cut(s) 34
BsuRI GGCC 1 cut(s) 384
BtsCI GGATG 1 cut(s) 270
BtsI GCAGTG 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 373
Csp6I GTAC 3 cut(s) 83, 173, 514
CviAII CATG 2 cut(s) 37, 261
CviJI RGCY 8 cut(s) 106, 114, 138, 316, 384, 458, 493, 508
CviKI_1 RGCY 8 cut(s) 106, 114, 138, 316, 384, 458, 493, 508
CviQI GTAC 3 cut(s) 83, 173, 514
DdeI CTNAG 3 cut(s) 167, 189, 317
DraI TTTAAA 1 cut(s) 526
EciI GGCGGA 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 1 cut(s) 379
Eco147I AGGCCT 1 cut(s) 384
Eco24I GRGCYC 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 371
EcoICRI GAGCTC 1 cut(s) 508
EcoNI CCTNNNNNAGG 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 379
EcoT22I ATGCAT 1 cut(s) 38
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 379
FaeI CATG 2 cut(s) 40, 264
FaiI YATR 3 cut(s) 38, 232, 262
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 2 cut(s) 36, 260
FokI GGATG 1 cut(s) 277
FriOI GRGCYC 1 cut(s) 510
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 1 cut(s) 384
Hin1II CATG 2 cut(s) 40, 264
HindIII AAGCTT 1 cut(s) 136
HphI GGTGA 4 cut(s) 74, 208, 310, 488
Hpy166II GTNNAC 3 cut(s) 12, 85, 406
Hpy188I TCNGA 1 cut(s) 190
Hpy8I GTNNAC 3 cut(s) 12, 85, 406
HpyCH4III ACNGT 1 cut(s) 76
HpyCH4IV ACGT 2 cut(s) 14, 234
HpyCH4V TGCA 3 cut(s) 36, 406, 433
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 98
HpyF3I CTNAG 3 cut(s) 167, 189, 317
HpySE526I ACGT 2 cut(s) 14, 234
Hsp92II CATG 2 cut(s) 40, 264
LmnI GCTCC 4 cut(s) 19, 103, 505, 513
LpnPI CCDG 6 cut(s) 101, 105, 128, 217, 269, 524
MaeII ACGT 2 cut(s) 14, 234
MaeIII GTNAC 2 cut(s) 206, 214
MboII GAAGA 1 cut(s) 380
MhlI GDGCHC 3 cut(s) 24, 408, 510
MmeI TCCRAC 2 cut(s) 58, 249
MnlI CCTC 3 cut(s) 62, 184, 358
Mph1103I ATGCAT 1 cut(s) 38
MseI TTAA 2 cut(s) 95, 525
MslI CAYNNNNRTG 1 cut(s) 259
MspR9I CCNGG 1 cut(s) 116
MvaI CCWGG 1 cut(s) 116
MwoI GCNNNNNNNGC 2 cut(s) 28, 98
NlaIII CATG 2 cut(s) 40, 264
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 38
PceI AGGCCT 1 cut(s) 384
Ppu21I YACGTR 1 cut(s) 235
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 240
RsaI GTAC 3 cut(s) 84, 174, 515
RsaNI GTAC 3 cut(s) 83, 173, 514
RseI CAYNNNNRTG 1 cut(s) 259
SacI GAGCTC 1 cut(s) 510
SaqAI TTAA 2 cut(s) 95, 525
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 3 cut(s) 24, 408, 510
SmiMI CAYNNNNRTG 1 cut(s) 259
SseBI AGGCCT 1 cut(s) 384
SsiI CCGC 3 cut(s) 101, 296, 390
SstI GAGCTC 1 cut(s) 510
StuI AGGCCT 1 cut(s) 384
StyD4I CCNGG 1 cut(s) 114
StyI CCWWGG 1 cut(s) 379
TaaI ACNGT 1 cut(s) 76
TaiI ACGT 2 cut(s) 17, 237
TaqI TCGA 1 cut(s) 6
TatI WGTACW 1 cut(s) 82
Tru1I TTAA 2 cut(s) 95, 525
Tru9I TTAA 2 cut(s) 95, 525
TscAI CASTG 1 cut(s) 380
TseFI GTSAC 1 cut(s) 214
Tsp45I GTSAC 1 cut(s) 214
TspRI CASTG 1 cut(s) 380
VneI GTGCAC 1 cut(s) 404
XagI CCTNNNNNAGG 1 cut(s) 166
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.