Rroxscaffold_3G00230670

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000751
Physical Location & Seq
Forward (+)
15593092 .. 15597576
4485 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00230670.1

Sequence Viewer

Length: 741 bp
ATGCCCAAAGTCCAGGACGTAAATGAGTGGCTAGAGTGGAGCATAGGTGCTTTGGATGTGATGCATCACCAGGCATCGGAGCTAAGATCTAAGGAACTGAGAGGCAAAGGAGATAGTGGCATTGAAGCCAAGACGTTGGAGCACCACAAGCAGTGCGTGGATACTTTTATCCTCTTGGGTGGTGATAAAGAAAAAGTTGGAGTACACAGTGCCTTAAAGTCGGAGCTGTTGAGCCTGGCAGGGTGTTTTTGTAGAAGCTTTTACCTTGACTTCCACATTTCCTTGCTTAGGGCACTACAAAAAAGGTCTGAAGCATTATTTTCCAGTAACAAAGTCACCCTTGAAAGCCTATACGTGGCACCTAAAAAGATGTCATTGTCATGGATGCTAGAGCATCAACAGCGGGTCGTGGAGGCGGTGATACAACTCGGTGGGCTTAGCAATGGCAAGACTACCCCAAAGTCCATGCTGAAGTTGATAGATGAGGAAGACCACCGCCAAGGCCTAACCGCAAAGATGATAGCGCACCACCTTGTAGGGTTGTATCATTTGCAAAAGGAAACTCAACGGCTAATGGCTTTTGGAATGGCCAGTGGTGAAAAAGAAACAGAGTTGTCGGTTAGGGAGCTCCAGTACCTTGTTTTTAAAGGATTGCCCCAACAACGAGATAACATTCTATACACAAATGTAAAAGATCCTCTACTTCGACAGTACCACATAGCCTATCAATCATTACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

246

Amino Acids

28.01

Weight (kDa)

8.33

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 358
AciI CCGC 4 cut(s) 403, 416, 496, 510
AclWI GGATC 1 cut(s) 689
AcoI YGGCCR 1 cut(s) 588
AcuI CTGAAG 2 cut(s) 330, 491
AfaI GTAC 3 cut(s) 204, 635, 713
AfiI CCNNNNNNNGG 3 cut(s) 76, 288, 355
AgsI TTSAA 2 cut(s) 125, 344
AjnI CCWGG 3 cut(s) 12, 69, 234
AluBI AGCT 4 cut(s) 82, 226, 258, 628
AluI AGCT 4 cut(s) 82, 226, 258, 628
Alw21I GWGCWC 2 cut(s) 144, 630
AlwI GGATC 1 cut(s) 689
AoxI GGCC 2 cut(s) 502, 588
AspLEI GCGC 1 cut(s) 526
AsuHPI GGTGA 5 cut(s) 59, 194, 328, 430, 608
BaeGI GKGCMC 1 cut(s) 295
BalI TGGCCA 1 cut(s) 590
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 144, 630
BceAI ACGGC 1 cut(s) 584
BciT130I CCWGG 3 cut(s) 14, 71, 236
BciVI GTATCC 1 cut(s) 154
BfaI CTAG 2 cut(s) 32, 389
BfuI GTATCC 1 cut(s) 154
BglII AGATCT 1 cut(s) 86
BlpI GCTNAGC 1 cut(s) 437
Bme1390I CCNGG 3 cut(s) 14, 71, 236
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 3 cut(s) 14, 71, 236
BmsI GCATC 5 cut(s) 51, 73, 83, 375, 403
BpiI GAAGAC 1 cut(s) 495
BpmI CTGGAG 1 cut(s) 614
Bpu10I CCTNAGC 1 cut(s) 287
Bpu1102I GCTNAGC 1 cut(s) 437
BsaAI YACGTR 1 cut(s) 355
BsaJI CCNNGG 1 cut(s) 499
BsaXI ACNNNNNCTCC 4 cut(s) 31, 61, 617, 647
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 288, 355
Bse1I ACTGG 3 cut(s) 324, 591, 631
Bse3DI GCAATG 1 cut(s) 448
BseBI CCWGG 3 cut(s) 14, 71, 236
BseDI CCNNGG 1 cut(s) 499
BseGI GGATG 2 cut(s) 61, 390
BseLI CCNNNNNNNGG 3 cut(s) 76, 288, 355
BseMI GCAATG 1 cut(s) 448
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 3 cut(s) 324, 591, 631
BseSI GKGCMC 1 cut(s) 295
BshFI GGCC 2 cut(s) 504, 590
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 144, 630
BslI CCNNNNNNNGG 3 cut(s) 76, 288, 355
BsnI GGCC 2 cut(s) 504, 590
Bsp1286I GDGCHC 3 cut(s) 144, 295, 630
Bsp143I GATC 2 cut(s) 86, 694
Bsp1720I GCTNAGC 1 cut(s) 437
BspACI CCGC 4 cut(s) 403, 416, 496, 510
BspANI GGCC 2 cut(s) 504, 590
BspCNI CTCAG 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 360
BspPI GGATC 1 cut(s) 689
BspT107I GGYRCC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 448
BsrI ACTGG 3 cut(s) 324, 591, 631
BssECI CCNNGG 1 cut(s) 499
BssMI GATC 2 cut(s) 86, 694
BssT1I CCWWGG 1 cut(s) 499
Bst2UI CCWGG 3 cut(s) 14, 71, 236
Bst4CI ACNGT 2 cut(s) 209, 711
BstBAI YACGTR 1 cut(s) 355
BstDEI CTNAG 5 cut(s) 83, 90, 98, 287, 437
BstENI CCTNNNNNAGG 1 cut(s) 286
BstF5I GGATG 2 cut(s) 61, 390
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 2 cut(s) 89, 697
BstMBI GATC 2 cut(s) 86, 694
BstMWI GCNNNNNNNGC 2 cut(s) 148, 400
BstNI CCWGG 3 cut(s) 14, 71, 236
BstSCI CCNGG 3 cut(s) 12, 69, 234
BstSLI GKGCMC 1 cut(s) 295
BstV2I GAAGAC 1 cut(s) 495
BstX2I RGATCY 2 cut(s) 86, 694
BstXI CCANNNNNNTGG 1 cut(s) 136
BstYI RGATCY 2 cut(s) 86, 694
BsuI GTATCC 1 cut(s) 154
BsuRI GGCC 2 cut(s) 504, 590
BtsCI GGATG 2 cut(s) 61, 390
BtsI GCAGTG 1 cut(s) 158
BtsIMutI CAGTG 3 cut(s) 158, 214, 598
CfoI GCGC 1 cut(s) 526
Csp6I GTAC 3 cut(s) 203, 634, 712
CviAII CATG 2 cut(s) 381, 466
CviQI GTAC 3 cut(s) 203, 634, 712
DdeI CTNAG 5 cut(s) 83, 90, 98, 287, 437
DpnI GATC 2 cut(s) 88, 696
DpnII GATC 2 cut(s) 86, 694
DraI TTTAAA 1 cut(s) 646
EaeI YGGCCR 1 cut(s) 588
Ecl136II GAGCTC 1 cut(s) 628
Eco130I CCWWGG 1 cut(s) 499
Eco147I AGGCCT 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 630
Eco53kI GAGCTC 1 cut(s) 628
Eco57I CTGAAG 2 cut(s) 330, 491
EcoICRI GAGCTC 1 cut(s) 628
EcoNI CCTNNNNNAGG 1 cut(s) 286
EcoRII CCWGG 3 cut(s) 12, 69, 234
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 630
ErhI CCWWGG 1 cut(s) 499
FaeI CATG 2 cut(s) 384, 469
FaiI YATR 6 cut(s) 44, 352, 382, 467, 679, 719
FalI AAGNNNNNCTT 2 cut(s) 324, 356
FatI CATG 2 cut(s) 380, 465
FauI CCCGC 1 cut(s) 396
FokI GGATG 2 cut(s) 68, 397
FriOI GRGCYC 1 cut(s) 630
FspBI CTAG 2 cut(s) 32, 389
GlaI GCGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 2 cut(s) 504, 590
HhaI GCGC 1 cut(s) 526
Hin1II CATG 2 cut(s) 384, 469
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HindIII AAGCTT 1 cut(s) 256
HphI GGTGA 5 cut(s) 59, 194, 328, 430, 608
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 3 cut(s) 79, 223, 310
Hpy8I GTNNAC 1 cut(s) 205
HpyCH4III ACNGT 2 cut(s) 209, 711
HpyCH4IV ACGT 3 cut(s) 18, 134, 354
HpyCH4V TGCA 2 cut(s) 64, 553
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 400
HpyF3I CTNAG 5 cut(s) 83, 90, 98, 287, 437
HpySE526I ACGT 3 cut(s) 18, 134, 354
Hsp92II CATG 2 cut(s) 384, 469
HspAI GCGC 1 cut(s) 524
Kzo9I GATC 2 cut(s) 86, 694
LmnI GCTCC 6 cut(s) 39, 79, 139, 223, 625, 633
LpnPI CCDG 9 cut(s) 26, 56, 83, 221, 225, 248, 337, 604, 644
LweI GCATC 5 cut(s) 51, 73, 83, 375, 403
MaeI CTAG 2 cut(s) 32, 389
MaeII ACGT 3 cut(s) 18, 134, 354
MaeIII GTNAC 2 cut(s) 326, 334
MalI GATC 2 cut(s) 88, 696
MboI GATC 2 cut(s) 86, 694
MboII GAAGA 1 cut(s) 500
MflI RGATCY 2 cut(s) 86, 694
MhlI GDGCHC 3 cut(s) 144, 295, 630
MlsI TGGCCA 1 cut(s) 590
MluNI TGGCCA 1 cut(s) 590
MmeI TCCRAC 3 cut(s) 117, 178, 201
MnlI CCTC 5 cut(s) 95, 182, 406, 478, 708
Mox20I TGGCCA 1 cut(s) 590
Mph1103I ATGCAT 1 cut(s) 66
MscI TGGCCA 1 cut(s) 590
MseI TTAA 2 cut(s) 215, 645
MslI CAYNNNNRTG 1 cut(s) 379
Msp20I TGGCCA 1 cut(s) 590
MspA1I CMGCKG 1 cut(s) 403
MspR9I CCNGG 3 cut(s) 14, 71, 236
MvaI CCWGG 3 cut(s) 14, 71, 236
MwoI GCNNNNNNNGC 2 cut(s) 148, 400
NdeII GATC 2 cut(s) 86, 694
NlaIII CATG 2 cut(s) 384, 469
NlaIV GGNNCC 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 66
PceI AGGCCT 1 cut(s) 504
PfoI TCCNGGA 1 cut(s) 12
Ppu21I YACGTR 1 cut(s) 355
Psp124BI GAGCTC 1 cut(s) 630
Psp6I CCWGG 3 cut(s) 12, 69, 234
PspGI CCWGG 3 cut(s) 12, 69, 234
PspN4I GGNNCC 1 cut(s) 360
PsuI RGATCY 2 cut(s) 86, 694
RsaI GTAC 3 cut(s) 204, 635, 713
RsaNI GTAC 3 cut(s) 203, 634, 712
RseI CAYNNNNRTG 1 cut(s) 379
SacI GAGCTC 1 cut(s) 630
SaqAI TTAA 2 cut(s) 215, 645
Sau3AI GATC 2 cut(s) 86, 694
ScrFI CCNGG 3 cut(s) 14, 71, 236
SduI GDGCHC 3 cut(s) 144, 295, 630
SfaNI GCATC 5 cut(s) 51, 73, 83, 375, 403
SmiMI CAYNNNNRTG 1 cut(s) 379
SseBI AGGCCT 1 cut(s) 504
SsiI CCGC 4 cut(s) 403, 416, 496, 510
SspMI CTAG 2 cut(s) 32, 389
SstI GAGCTC 1 cut(s) 630
StuI AGGCCT 1 cut(s) 504
StyD4I CCNGG 3 cut(s) 12, 69, 234
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 2 cut(s) 209, 711
TaiI ACGT 3 cut(s) 21, 137, 357
TaqI TCGA 1 cut(s) 706
TatI WGTACW 1 cut(s) 202
Tru1I TTAA 2 cut(s) 215, 645
Tru9I TTAA 2 cut(s) 215, 645
TscAI CASTG 3 cut(s) 158, 214, 598
TseFI GTSAC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 334
TspRI CASTG 3 cut(s) 158, 214, 598
XagI CCTNNNNNAGG 1 cut(s) 286
XspI CTAG 2 cut(s) 32, 389
Zsp2I ATGCAT 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.