Rorug07G0256300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000751
Physical Location & Seq
Reverse (-)
23945881 .. 23946948
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0256300.1

Sequence Viewer

Length: 1068 bp
ATGGAGGAGGTGATTGGGACTTTCGCAAGTAAGATGGCGTTGGAGGCTAATGTTGTTGAGGTGATCCTTCCGGAGGATGTCAACTCGGAGAAGCAGCGACGTTTCTACATGGTGGGGGAGTTGCTTACATGTAGATCCTATAACAAGGAGGCGCTGATCAGTACTCTGGGGGCGTTGTGGACTCCAAAGTCGGGGCCGGCAGATCGGTCAAAGTTGACGATCTGTACATTGGATGGGAGTGATCAGCTACTGATTTCTTTCAAGTTTGAATCGGATCTCAAATGGGTTGTCAAAGGATGCCCATGGACTTTTGAGAAGTCGTTGTTCGCGGTGGCGGTGATGAATGGTATGGAGGATCCTTTGAGGGTGTCTTTGGCCACCCAATATTTTTGGGTTAGGGTTCGTGGTCTACCGCCGAGTTACATGAAGGAAGATACAGGGAAAGGCATTGGGACGGCGATAGGGCATTATGTTCGAACCCAAGTTGACAGACAAGGTCTTTGCTTGGGTAGCTATCTTCGTTTCAGGGTGGGAATTAATGTGGAGGTGCCTCTAGTGAGTGAGGTGGATTTCAAGGCGGCGGGTTGGCCAGTGGGTAAACGGTATGGGGTGGAGTATGAGAGACTCCCCCACTTCTGTCTTTTCTGTGGGTTATTATCTCACACAGGCTTTCGGTGTCCGGACAGGGAGGCGGGGGTTGTGTCGGAGCCGGTCTATAGTGTGTTGCTGAACGCAGAGAAGAAAGAAAAATGGCTGGAGTTACAACGTCAGCAAGGGGAGCATTCCGGTGGACTCTCTGCACAACGTGGGGAGGGGCGGCGGTTTGGCTTATTCCCTCAGAAGAAAAGTGGTTGGTCGATGGTAGAGGTGGCAAACGGGCCTAGTTGTGCTTCGTGCCGTGCTTGGGCCGGCCCATTTATTATCGTGCCGGGCTCGTGCCGGCCCATTTACTTAAACATTAAGCCCGGTCCGGCCCATGGCCCGAGCCCATATCGTGCCGGGCCGTGCTCGTGCCGGGTCAATAAACGGGCCGTGCTCGTGCCTACCCACTATAAAGCACGATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

355

Amino Acids

39.49

Weight (kDa)

9.14

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 187
AccB1I GGYRCC 1 cut(s) 547
AccI GTMKAC 1 cut(s) 409
AccII CGCG 1 cut(s) 329
AccIII TCCGGA 2 cut(s) 70, 679
AciI CCGC 8 cut(s) 329, 335, 413, 578, 581, 692, 817, 820
AclWI GGATC 5 cut(s) 58, 129, 282, 350, 363
AcoI YGGCCR 2 cut(s) 375, 587
AdeI CACNNNGTG 1 cut(s) 806
AfaI GTAC 2 cut(s) 163, 226
AfiI CCNNNNNNNGG 4 cut(s) 73, 191, 904, 977
AflIII ACRYGT 1 cut(s) 128
AgsI TTSAA 3 cut(s) 262, 269, 574
AluBI AGCT 2 cut(s) 247, 513
AluI AGCT 2 cut(s) 247, 513
Alw21I GWGCWC 2 cut(s) 1010, 1038
Alw26I GTCTC 1 cut(s) 616
AlwI GGATC 5 cut(s) 58, 129, 282, 350, 363
AlwNI CAGNNNCTG 1 cut(s) 250
Ama87I CYCGRG 1 cut(s) 982
Aor13HI TCCGGA 2 cut(s) 70, 679
ApeKI GCWGC 1 cut(s) 94
ArsI GACNNNNNNTTYG 4 cut(s) 273, 305, 483, 515
AseI ATTAAT 1 cut(s) 537
AspLEI GCGC 1 cut(s) 154
AsuC2I CCSGG 4 cut(s) 930, 966, 1000, 1016
AsuHPI GGTGA 3 cut(s) 22, 73, 349
AsuII TTCGAA 1 cut(s) 475
AvaI CYCGRG 1 cut(s) 982
AvaII GGWCC 1 cut(s) 968
BalI TGGCCA 2 cut(s) 377, 589
BamHI GGATCC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 547
BanII GRGCYC 2 cut(s) 935, 989
BauI CACGAG 3 cut(s) 934, 1009, 1037
Bbv12I GWGCWC 2 cut(s) 1010, 1038
BbvI GCAGC 1 cut(s) 106
BccI CCATC 3 cut(s) 28, 227, 853
BceAI ACGGC 4 cut(s) 471, 882, 988, 1016
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BclI TGATCA 2 cut(s) 156, 241
BcnI CCSGG 4 cut(s) 930, 966, 1000, 1016
BcoDI GTCTC 1 cut(s) 616
BfaI CTAG 2 cut(s) 554, 882
BfmI CTRYAG 1 cut(s) 715
BfoI RGCGCY 1 cut(s) 155
BisI GCNGC 3 cut(s) 95, 579, 818
BlsI GCNGC 3 cut(s) 96, 580, 819
BmcAI AGTACT 1 cut(s) 163
Bme1390I CCNGG 4 cut(s) 930, 966, 1000, 1016
Bme18I GGWCC 1 cut(s) 968
BmeT110I CYCGRG 1 cut(s) 982
BmiI GGNNCC 4 cut(s) 195, 357, 549, 708
BmrFI CCNGG 4 cut(s) 930, 966, 1000, 1016
BmsI GCATC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 776
Bpu14I TTCGAA 1 cut(s) 475
BpuMI CCSGG 4 cut(s) 930, 966, 1000, 1016
BsaJI CCNNGG 2 cut(s) 302, 976
BsaWI WCCGGW 3 cut(s) 70, 679, 785
Bsc4I CCNNNNNNNGG 4 cut(s) 73, 191, 904, 977
Bse118I RCCGGY 4 cut(s) 196, 709, 908, 939
Bse1I ACTGG 1 cut(s) 590
BseAI TCCGGA 2 cut(s) 70, 679
BseDI CCNNGG 2 cut(s) 302, 976
BseGI GGATG 3 cut(s) 82, 238, 302
BseLI CCNNNNNNNGG 4 cut(s) 73, 191, 904, 977
BseMII CTCAG 1 cut(s) 851
BseNI ACTGG 1 cut(s) 590
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 106
BsgI GTGCAG 1 cut(s) 783
Bsh1236I CGCG 1 cut(s) 329
BshNI GGYRCC 1 cut(s) 547
BsiHKAI GWGCWC 2 cut(s) 1010, 1038
BsiHKCI CYCGRG 1 cut(s) 982
BslFI GGGAC 2 cut(s) 31, 466
BslI CCNNNNNNNGG 4 cut(s) 73, 191, 904, 977
BsmAI GTCTC 1 cut(s) 616
BsmFI GGGAC 2 cut(s) 31, 466
BsmI GAATGC 1 cut(s) 781
BsoBI CYCGRG 1 cut(s) 982
Bsp119I TTCGAA 1 cut(s) 475
Bsp1286I GDGCHC 4 cut(s) 935, 989, 1010, 1038
Bsp13I TCCGGA 2 cut(s) 70, 679
Bsp1407I TGTACA 1 cut(s) 224
Bsp143I GATC 8 cut(s) 63, 134, 156, 202, 219, 241, 274, 355
Bsp19I CCATGG 2 cut(s) 302, 976
BspACI CCGC 8 cut(s) 329, 335, 413, 578, 581, 692, 817, 820
BspCNI CTCAG 1 cut(s) 850
BspEI TCCGGA 2 cut(s) 70, 679
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 4 cut(s) 195, 357, 549, 708
BspPI GGATC 5 cut(s) 58, 129, 282, 350, 363
BspT104I TTCGAA 1 cut(s) 475
BspT107I GGYRCC 1 cut(s) 547
BsrFI RCCGGY 4 cut(s) 196, 709, 908, 939
BsrGI TGTACA 1 cut(s) 224
BsrI ACTGG 1 cut(s) 590
BssAI RCCGGY 4 cut(s) 196, 709, 908, 939
BssECI CCNNGG 2 cut(s) 302, 976
BssMI GATC 8 cut(s) 63, 134, 156, 202, 219, 241, 274, 355
BssSI CACGAG 3 cut(s) 934, 1009, 1037
BssT1I CCWWGG 2 cut(s) 302, 976
Bst2BI CACGAG 3 cut(s) 934, 1009, 1037
Bst4CI ACNGT 1 cut(s) 603
BstAUI TGTACA 1 cut(s) 224
BstBI TTCGAA 1 cut(s) 475
BstC8I GCNNGC 3 cut(s) 198, 910, 941
BstDEI CTNAG 1 cut(s) 837
BstDSI CCRYGG 2 cut(s) 302, 976
BstENI CCTNNNNNAGG 1 cut(s) 71
BstF5I GGATG 3 cut(s) 82, 238, 302
BstFNI CGCG 1 cut(s) 329
BstH2I RGCGCY 1 cut(s) 155
BstHHI GCGC 1 cut(s) 154
BstKTI GATC 8 cut(s) 66, 137, 159, 205, 222, 244, 277, 358
BstMAI GTCTC 1 cut(s) 616
BstMBI GATC 8 cut(s) 63, 134, 156, 202, 219, 241, 274, 355
BstMWI GCNNNNNNNGC 3 cut(s) 44, 510, 778
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 4 cut(s) 928, 964, 998, 1014
BstSFI CTRYAG 1 cut(s) 715
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 106
BstX2I RGATCY 3 cut(s) 134, 274, 355
BstYI RGATCY 3 cut(s) 134, 274, 355
BtgI CCRYGG 2 cut(s) 302, 976
BtsCI GGATG 3 cut(s) 82, 238, 302
BtsIMutI CAGTG 1 cut(s) 597
Cac8I GCNNGC 3 cut(s) 198, 910, 941
CaiI CAGNNNCTG 1 cut(s) 250
CfoI GCGC 1 cut(s) 154
Cfr10I RCCGGY 4 cut(s) 196, 709, 908, 939
CpoI CGGWCCG 1 cut(s) 968
Csp6I GTAC 2 cut(s) 162, 225
CspI CGGWCCG 1 cut(s) 968
CviAII CATG 5 cut(s) 109, 129, 303, 424, 977
CviQI GTAC 2 cut(s) 162, 225
DdeI CTNAG 1 cut(s) 837
DpnI GATC 8 cut(s) 65, 136, 158, 204, 221, 243, 276, 357
DpnII GATC 8 cut(s) 63, 134, 156, 202, 219, 241, 274, 355
DraIII CACNNNGTG 1 cut(s) 806
DrdI GACNNNNNNGTC 1 cut(s) 187
DseDI GACNNNNNNGTC 1 cut(s) 187
EaeI YGGCCR 2 cut(s) 375, 587
Eco130I CCWWGG 2 cut(s) 302, 976
Eco24I GRGCYC 2 cut(s) 935, 989
Eco47I GGWCC 1 cut(s) 968
Eco88I CYCGRG 1 cut(s) 982
EcoNI CCTNNNNNAGG 1 cut(s) 71
EcoT14I CCWWGG 2 cut(s) 302, 976
EcoT38I GRGCYC 2 cut(s) 935, 989
ErhI CCWWGG 2 cut(s) 302, 976
FaeI CATG 5 cut(s) 112, 132, 306, 427, 980
FaqI GGGAC 2 cut(s) 31, 466
FatI CATG 5 cut(s) 108, 128, 302, 423, 976
FauI CCCGC 2 cut(s) 574, 685
FbaI TGATCA 2 cut(s) 156, 241
FblI GTMKAC 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 95, 579, 818
FokI GGATG 3 cut(s) 89, 245, 309
FriOI GRGCYC 2 cut(s) 935, 989
FseI GGCCGGCC 1 cut(s) 912
Fsp4HI GCNGC 3 cut(s) 95, 579, 818
FspBI CTAG 2 cut(s) 554, 882
GlaI GCGC 1 cut(s) 153
GluI GCNGC 3 cut(s) 95, 579, 818
GsuI CTGGAG 1 cut(s) 776
HaeII RGCGCY 1 cut(s) 155
HhaI GCGC 1 cut(s) 154
Hin1II CATG 5 cut(s) 112, 132, 306, 427, 980
Hin6I GCGC 1 cut(s) 152
HinP1I GCGC 1 cut(s) 152
HincII GTYRAC 3 cut(s) 82, 216, 487
HindII GTYRAC 3 cut(s) 82, 216, 487
HinfI GANTC 4 cut(s) 181, 269, 624, 792
HphI GGTGA 3 cut(s) 22, 73, 349
Hpy166II GTNNAC 7 cut(s) 82, 180, 216, 410, 487, 599, 791
Hpy188I TCNGA 4 cut(s) 88, 274, 706, 840
Hpy188III TCNNGA 2 cut(s) 71, 680
Hpy8I GTNNAC 7 cut(s) 82, 180, 216, 410, 487, 599, 791
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 2 cut(s) 77, 421
HpyCH4III ACNGT 1 cut(s) 603
HpyCH4IV ACGT 3 cut(s) 100, 766, 805
HpyCH4V TGCA 1 cut(s) 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 510, 778
HpyF3I CTNAG 1 cut(s) 837
HpySE526I ACGT 3 cut(s) 100, 766, 805
Hsp92II CATG 5 cut(s) 112, 132, 306, 427, 980
HspAI GCGC 1 cut(s) 152
Kpn2I TCCGGA 2 cut(s) 70, 679
KroI GCCGGC 3 cut(s) 196, 908, 939
KroNI GCCGGC 3 cut(s) 198, 910, 941
Ksp22I TGATCA 2 cut(s) 156, 241
Kzo9I GATC 8 cut(s) 63, 134, 156, 202, 219, 241, 274, 355
LmnI GCTCC 2 cut(s) 706, 778
Lsp1109I GCAGC 1 cut(s) 106
LweI GCATC 1 cut(s) 287
MaeI CTAG 2 cut(s) 554, 882
MaeII ACGT 3 cut(s) 100, 766, 805
MaeIII GTNAC 2 cut(s) 419, 759
MalI GATC 8 cut(s) 65, 136, 158, 204, 221, 243, 276, 357
MboI GATC 8 cut(s) 63, 134, 156, 202, 219, 241, 274, 355
MboII GAAGA 4 cut(s) 443, 509, 751, 853
MflI RGATCY 3 cut(s) 134, 274, 355
MhlI GDGCHC 4 cut(s) 935, 989, 1010, 1038
MlsI TGGCCA 2 cut(s) 377, 589
MluCI AATT 1 cut(s) 534
MluNI TGGCCA 2 cut(s) 377, 589
MlyI GAGTC 3 cut(s) 175, 618, 786
MmeI TCCRAC 2 cut(s) 21, 684
Mox20I TGGCCA 2 cut(s) 377, 589
MroI TCCGGA 2 cut(s) 70, 679
MroNI GCCGGC 3 cut(s) 196, 908, 939
MscI TGGCCA 2 cut(s) 377, 589
MseI TTAA 4 cut(s) 537, 953, 960, 1066
MslI CAYNNNNRTG 1 cut(s) 786
Msp20I TGGCCA 2 cut(s) 377, 589
MspR9I CCNGG 4 cut(s) 930, 966, 1000, 1016
Mva1269I GAATGC 1 cut(s) 781
MvnI CGCG 1 cut(s) 329
MwoI GCNNNNNNNGC 3 cut(s) 44, 510, 778
NaeI GCCGGC 3 cut(s) 198, 910, 941
NciI CCSGG 4 cut(s) 930, 966, 1000, 1016
NcoI CCATGG 2 cut(s) 302, 976
NdeII GATC 8 cut(s) 63, 134, 156, 202, 219, 241, 274, 355
NgoMIV GCCGGC 3 cut(s) 196, 908, 939
NlaIII CATG 5 cut(s) 112, 132, 306, 427, 980
NlaIV GGNNCC 4 cut(s) 195, 357, 549, 708
NmeAIII GCCGAG 1 cut(s) 441
NspI RCATGY 1 cut(s) 132
NspV TTCGAA 1 cut(s) 475
PciI ACATGT 1 cut(s) 128
PctI GAATGC 1 cut(s) 781
PdiI GCCGGC 3 cut(s) 198, 910, 941
PfeI GAWTC 1 cut(s) 269
PflFI GACNNNGTC 1 cut(s) 495
PkrI GCNGC 3 cut(s) 96, 580, 819
PleI GAGTC 3 cut(s) 175, 618, 786
PpsI GAGTC 3 cut(s) 175, 618, 786
PscI ACATGT 1 cut(s) 128
PshBI ATTAAT 1 cut(s) 537
PspN4I GGNNCC 4 cut(s) 195, 357, 549, 708
PstNI CAGNNNCTG 1 cut(s) 250
PsuI RGATCY 3 cut(s) 134, 274, 355
PsyI GACNNNGTC 1 cut(s) 495
RigI GGCCGGCC 1 cut(s) 912
RsaI GTAC 2 cut(s) 163, 226
RsaNI GTAC 2 cut(s) 162, 225
RseI CAYNNNNRTG 1 cut(s) 786
Rsr2I CGGWCCG 1 cut(s) 968
RsrII CGGWCCG 1 cut(s) 968
SaqAI TTAA 4 cut(s) 537, 953, 960, 1066
SatI GCNGC 3 cut(s) 95, 579, 818
Sau3AI GATC 8 cut(s) 63, 134, 156, 202, 219, 241, 274, 355
ScaI AGTACT 1 cut(s) 163
SchI GAGTC 3 cut(s) 175, 618, 786
ScrFI CCNGG 4 cut(s) 930, 966, 1000, 1016
SduI GDGCHC 4 cut(s) 935, 989, 1010, 1038
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 1 cut(s) 715
SfuI TTCGAA 1 cut(s) 475
SinI GGWCC 1 cut(s) 968
SmiMI CAYNNNNRTG 1 cut(s) 786
Sse9I AATT 1 cut(s) 534
SsiI CCGC 8 cut(s) 329, 335, 413, 578, 581, 692, 817, 820
SspI AATATT 1 cut(s) 386
SspMI CTAG 2 cut(s) 554, 882
StyD4I CCNGG 4 cut(s) 928, 964, 998, 1014
StyI CCWWGG 2 cut(s) 302, 976
TaaI ACNGT 1 cut(s) 603
TaiI ACGT 3 cut(s) 103, 769, 808
TaqI TCGA 2 cut(s) 475, 857
TaqII GACCGA 1 cut(s) 195
TasI AATT 1 cut(s) 534
TatI WGTACW 2 cut(s) 161, 224
TauI GCSGC 2 cut(s) 581, 820
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 4 cut(s) 537, 953, 960, 1066
Tru9I TTAA 4 cut(s) 537, 953, 960, 1066
TscAI CASTG 1 cut(s) 597
TseI GCWGC 1 cut(s) 94
TspDTI ATGAA 2 cut(s) 356, 440
TspRI CASTG 1 cut(s) 597
Tth111I GACNNNGTC 1 cut(s) 495
VpaK11BI GGWCC 1 cut(s) 968
VspI ATTAAT 1 cut(s) 537
XagI CCTNNNNNAGG 1 cut(s) 71
XceI RCATGY 1 cut(s) 132
XmiI GTMKAC 1 cut(s) 409
XspI CTAG 2 cut(s) 554, 882
ZrmI AGTACT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.