RLG00000027399

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000751
Physical Location & Seq
Reverse (-)
11225301 .. 11226792
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027399

Sequence Viewer

Length: 462 bp
ATGGAACAACTACAGAAGCAAACTCAATCAGTTCTATTACGTCGTCCACCCCGAAAACCATCTTGTAAACTCAGTGTTTTGGTGGACGATTTGGTGAGAGACTTGGATGGTCTAATCATGGAACGTAAGCTGTCAACGTGGACACTCGAGCATCACAAGCGTGTGGTGGATGGTTTGGAAAAGGTGGGTGGCTTTGACATGGCAAACCCCATGGAGAAGACGGCGGTTAGGGTTTTGAGATTGATGGAAGAAGAGCGCCGCCAGGGTCTCACCGCATGGATGGTAACGAAGCATCTGATAGGGTTGCGCTATTTACAGAGGAGAACTCAGGAGGCCATTGTTAAAGTGGCAGAGAATAAAGCTAAACATGGCTACAGCGTCAGGAGGCCCAAAAAAGTGAAGCCTGTGAGCATCGACGACGACGGTGTCGTTCCGGGTGAGGTGTTGTTCAGCGGCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.49

Weight (kDa)

9.89

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
AciI CCGC 5 cut(s) 224, 259, 273, 453, 456
AjnI CCWGG 1 cut(s) 261
AleI CACNNNNGTG 1 cut(s) 159
AluBI AGCT 2 cut(s) 130, 362
AluI AGCT 2 cut(s) 130, 362
Alw26I GTCTC 2 cut(s) 93, 272
Ama87I CYCGRG 1 cut(s) 146
AoxI GGCC 2 cut(s) 333, 386
AspLEI GCGC 2 cut(s) 258, 309
AspS9I GGNCC 1 cut(s) 387
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 3 cut(s) 106, 262, 449
AvaI CYCGRG 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 224
BccI CCATC 5 cut(s) 67, 101, 164, 238, 274
BceAI ACGGC 1 cut(s) 237
BciT130I CCWGG 1 cut(s) 263
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 2 cut(s) 93, 272
BfmI CTRYAG 2 cut(s) 11, 373
BfoI RGCGCY 1 cut(s) 259
BisI GCNGC 3 cut(s) 259, 454, 457
BlsI GCNGC 3 cut(s) 260, 455, 458
Bme1390I CCNGG 2 cut(s) 263, 435
BmeT110I CYCGRG 1 cut(s) 146
BmgT120I GGNCC 1 cut(s) 387
BmrFI CCNGG 2 cut(s) 263, 435
BmsI GCATC 3 cut(s) 160, 301, 420
BpiI GAAGAC 1 cut(s) 224
BplI GAGNNNNNCTC 2 cut(s) 310, 342
BpuMI CCSGG 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 210, 262
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 2 cut(s) 210, 262
BseGI GGATG 3 cut(s) 112, 175, 285
BseMII CTCAG 2 cut(s) 85, 341
BseRI GAGGAG 1 cut(s) 334
BshFI GGCC 2 cut(s) 335, 388
BsiHKCI CYCGRG 1 cut(s) 146
BsiSI CCGG 1 cut(s) 434
BsmAI GTCTC 2 cut(s) 93, 272
BsnI GGCC 2 cut(s) 335, 388
Bso31I GGTCTC 1 cut(s) 272
BsoBI CYCGRG 1 cut(s) 146
Bsp19I CCATGG 1 cut(s) 210
BspACI CCGC 5 cut(s) 224, 259, 273, 453, 456
BspANI GGCC 2 cut(s) 335, 388
BspCNI CTCAG 2 cut(s) 84, 340
BspQI GCTCTTC 1 cut(s) 246
BspTNI GGTCTC 1 cut(s) 272
BssECI CCNNGG 2 cut(s) 210, 262
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 71, 327
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 3 cut(s) 112, 175, 285
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 2 cut(s) 258, 309
BstMAI GTCTC 2 cut(s) 93, 272
BstMWI GCNNNNNNNGC 1 cut(s) 157
BstNI CCWGG 1 cut(s) 263
BstSCI CCNGG 2 cut(s) 261, 433
BstSFI CTRYAG 2 cut(s) 11, 373
BstV2I GAAGAC 1 cut(s) 224
BsuRI GGCC 2 cut(s) 335, 388
BtgI CCRYGG 1 cut(s) 210
BtsCI GGATG 3 cut(s) 112, 175, 285
BtsIMutI CAGTG 1 cut(s) 79
CfoI GCGC 2 cut(s) 258, 309
Cfr13I GGNCC 1 cut(s) 387
CseI GACGC 1 cut(s) 367
CviAII CATG 5 cut(s) 118, 199, 211, 276, 368
CviJI RGCY 8 cut(s) 130, 192, 335, 362, 372, 388, 403, 459
CviKI_1 RGCY 8 cut(s) 130, 192, 335, 362, 372, 388, 403, 459
DdeI CTNAG 2 cut(s) 71, 327
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
FaeI CATG 5 cut(s) 121, 202, 214, 279, 371
FaiI YATR 5 cut(s) 119, 200, 212, 277, 369
FatI CATG 5 cut(s) 117, 198, 210, 275, 367
Fnu4HI GCNGC 3 cut(s) 259, 454, 457
FokI GGATG 3 cut(s) 119, 182, 292
Fsp4HI GCNGC 3 cut(s) 259, 454, 457
GlaI GCGC 2 cut(s) 257, 308
GluI GCNGC 3 cut(s) 259, 454, 457
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 2 cut(s) 335, 388
HapII CCGG 1 cut(s) 434
HgaI GACGC 1 cut(s) 367
HhaI GCGC 2 cut(s) 258, 309
Hin1II CATG 5 cut(s) 121, 202, 214, 279, 371
Hin6I GCGC 2 cut(s) 256, 307
HinP1I GCGC 2 cut(s) 256, 307
HincII GTYRAC 1 cut(s) 135
HindII GTYRAC 1 cut(s) 135
HpaII CCGG 1 cut(s) 434
HphI GGTGA 3 cut(s) 106, 262, 449
Hpy166II GTNNAC 5 cut(s) 47, 68, 85, 135, 141
Hpy188I TCNGA 1 cut(s) 297
Hpy188III TCNNGA 2 cut(s) 329, 382
Hpy8I GTNNAC 5 cut(s) 47, 68, 85, 135, 141
Hpy99I CGWCG 4 cut(s) 45, 419, 422, 425
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4IV ACGT 3 cut(s) 40, 124, 137
HpyF10VI GCNNNNNNNGC 1 cut(s) 157
HpyF3I CTNAG 2 cut(s) 71, 327
HpySE526I ACGT 3 cut(s) 40, 124, 137
Hsp92II CATG 5 cut(s) 121, 202, 214, 279, 371
HspAI GCGC 2 cut(s) 256, 307
LguI GCTCTTC 1 cut(s) 246
LpnPI CCDG 6 cut(s) 248, 275, 314, 367, 417, 447
LweI GCATC 3 cut(s) 160, 301, 420
MaeII ACGT 3 cut(s) 40, 124, 137
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 3 cut(s) 229, 260, 263
MnlI CCTC 4 cut(s) 312, 325, 378, 433
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 453
MspI CCGG 1 cut(s) 434
MspR9I CCNGG 2 cut(s) 263, 435
MvaI CCWGG 1 cut(s) 263
MwoI GCNNNNNNNGC 1 cut(s) 157
NciI CCSGG 1 cut(s) 435
NcoI CCATGG 1 cut(s) 210
NlaIII CATG 5 cut(s) 121, 202, 214, 279, 371
OliI CACNNNNGTG 1 cut(s) 159
PaeR7I CTCGAG 1 cut(s) 146
PciSI GCTCTTC 1 cut(s) 246
PcsI WCGNNNNNNNCGW 2 cut(s) 49, 426
PflFI GACNNNGTC 1 cut(s) 425
PkrI GCNGC 3 cut(s) 260, 455, 458
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspPI GGNCC 1 cut(s) 387
PspXI VCTCGAGB 1 cut(s) 146
PsyI GACNNNGTC 1 cut(s) 425
RseI CAYNNNNRTG 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 3 cut(s) 259, 454, 457
Sau96I GGNCC 1 cut(s) 387
ScrFI CCNGG 2 cut(s) 263, 435
SetI ASST 7 cut(s) 43, 127, 132, 140, 186, 364, 444
SfaNI GCATC 3 cut(s) 160, 301, 420
SfcI CTRYAG 2 cut(s) 11, 373
Sfr274I CTCGAG 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 146
SmoI CTYRAG 1 cut(s) 146
SsiI CCGC 5 cut(s) 224, 259, 273, 453, 456
StyD4I CCNGG 2 cut(s) 261, 433
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 1 cut(s) 425
TaiI ACGT 3 cut(s) 43, 127, 140
TaqI TCGA 2 cut(s) 147, 414
TauI GCSGC 3 cut(s) 261, 456, 459
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 1 cut(s) 79
TspRI CASTG 1 cut(s) 79
Tth111I GACNNNGTC 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.