Rh1AG333100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000751
Physical Location & Seq
Forward (+)
56800398 .. 56801328
931 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG333100.1

Sequence Viewer

Length: 585 bp
ATGGAAGTCACCGGCGGAGAAACCAGAAGAGACCCTTTGATCATAGGGTGGACGCTGGAGCACCACAAGCAGTTTTTAGAGTCGGTGGTGCGCAACGGTGGTTTTAACAAACCCTCAATTACTCCAATAGTAGTGGTGGACGTGCTGAACAAGGTGAACGGTCTTCGCCCGTATCCTATTGTGTATCACATGTTCTGGTTCCGAAAATTCCACAAAGAGAATGAATCCCTATTCAAGAAGTTGGCTGAGTTTGATTCTTTGGTCAGAGATTTTGCAGGCCTAGGAGTGGAAGGAGCAAAGTGCTGGTCGTCATGGACAGTAGAGCATTACAAGGAGGTGGTGGAGGCTATTATACAGCTGGGTGGCCTAGGCAACGAAACACCAGCGAAGCTCCTGGAGTTGGTGGACCGCCGCCTTGGCCTCACAGTAGAGATTCTAGAGCACATACTTTCGAGGTTGGAAACTTTGCAGGAAAGAACTCAAGAAGCTACGTATGGCCACGGCGGTCCACTGCGGCCGGGCAGGCAGAGAGTGAGTGGTAATGTAGTTCATCCTTACACTCGAGAGAAAAATAATAAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

22.21

Weight (kDa)

8.99

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 92
AciI CCGC 5 cut(s) 15, 409, 412, 504, 514
AcoI YGGCCR 2 cut(s) 496, 515
AcsI RAATTY 1 cut(s) 206
AfiI CCNNNNNNNGG 2 cut(s) 286, 400
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 1 cut(s) 235
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 1 cut(s) 393
AluBI AGCT 3 cut(s) 358, 391, 488
AluI AGCT 3 cut(s) 358, 391, 488
Alw21I GWGCWC 2 cut(s) 63, 444
Alw26I GTCTC 1 cut(s) 24
Ama87I CYCGRG 1 cut(s) 561
AoxI GGCC 5 cut(s) 277, 364, 418, 496, 515
ApoI RAATTY 1 cut(s) 206
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AspA2I CCTAGG 2 cut(s) 280, 367
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 2 cut(s) 406, 506
AsuC2I CCSGG 1 cut(s) 519
AsuHPI GGTGA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 2 cut(s) 406, 506
AvrII CCTAGG 2 cut(s) 280, 367
BalI TGGCCA 1 cut(s) 498
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 2 cut(s) 63, 444
BceAI ACGGC 1 cut(s) 517
BciT130I CCWGG 1 cut(s) 395
BciVI GTATCC 1 cut(s) 183
BclI TGATCA 1 cut(s) 39
BcnI CCSGG 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 3 cut(s) 281, 368, 437
BfuI GTATCC 1 cut(s) 183
BglI GCCNNNNNGGC 2 cut(s) 417, 523
BisI GCNGC 2 cut(s) 412, 515
BlnI CCTAGG 2 cut(s) 280, 367
BlsI GCNGC 2 cut(s) 413, 516
Bme1390I CCNGG 2 cut(s) 395, 519
Bme18I GGWCC 2 cut(s) 406, 506
BmeT110I CYCGRG 1 cut(s) 561
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 406, 506
BmiI GGNNCC 1 cut(s) 200
BmrFI CCNGG 2 cut(s) 395, 519
BpiI GAAGAC 1 cut(s) 155
BpmI CTGGAG 2 cut(s) 77, 416
BpuEI CTTGAG 1 cut(s) 465
BpuMI CCSGG 1 cut(s) 519
BsaAI YACGTR 1 cut(s) 492
BsaI GGTCTC 1 cut(s) 24
BsaJI CCNNGG 4 cut(s) 280, 367, 415, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 400
Bse118I RCCGGY 1 cut(s) 11
BseBI CCWGG 1 cut(s) 395
BseDI CCNNGG 4 cut(s) 280, 367, 415, 499
BseGI GGATG 1 cut(s) 550
BseLI CCNNNNNNNGG 2 cut(s) 286, 400
BseMII CTCAG 1 cut(s) 237
BseX3I CGGCCG 1 cut(s) 515
BseYI CCCAGC 1 cut(s) 358
Bsh1285I CGRYCG 1 cut(s) 518
BshFI GGCC 5 cut(s) 279, 366, 420, 498, 517
BsiEI CGRYCG 1 cut(s) 518
BsiHKAI GWGCWC 2 cut(s) 63, 444
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 2 cut(s) 12, 518
BslI CCNNNNNNNGG 2 cut(s) 286, 400
BsmAI GTCTC 1 cut(s) 24
BsnI GGCC 5 cut(s) 279, 366, 420, 498, 517
Bso31I GGTCTC 1 cut(s) 24
BsoBI CYCGRG 1 cut(s) 561
Bsp1286I GDGCHC 2 cut(s) 63, 444
Bsp143I GATC 1 cut(s) 39
BspACI CCGC 5 cut(s) 15, 409, 412, 504, 514
BspANI GGCC 5 cut(s) 279, 366, 420, 498, 517
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 24
BsrFI RCCGGY 1 cut(s) 11
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 4 cut(s) 280, 367, 415, 499
BssMI GATC 1 cut(s) 39
BssT1I CCWWGG 3 cut(s) 280, 367, 415
Bst2UI CCWGG 1 cut(s) 395
Bst4CI ACNGT 4 cut(s) 98, 161, 319, 427
Bst6I CTCTTC 1 cut(s) 22
BstBAI YACGTR 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 277, 524
BstDEI CTNAG 1 cut(s) 246
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 1 cut(s) 550
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 1 cut(s) 42
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 1 cut(s) 39
BstMCI CGRYCG 1 cut(s) 518
BstMWI GCNNNNNNNGC 3 cut(s) 67, 417, 523
BstNI CCWGG 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 393, 517
BstSNI TACGTA 1 cut(s) 492
BstV2I GAAGAC 1 cut(s) 155
BstZI CGGCCG 1 cut(s) 515
BsuI GTATCC 1 cut(s) 183
BsuRI GGCC 5 cut(s) 279, 366, 420, 498, 517
BtgI CCRYGG 1 cut(s) 499
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 1 cut(s) 550
BtsI GCAGTG 1 cut(s) 509
BtsIMutI CAGTG 1 cut(s) 509
Cac8I GCNNGC 2 cut(s) 277, 524
CfoI GCGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 406, 506
CseI GACGC 1 cut(s) 61
CspCI CAANNNNNGTGG 2 cut(s) 114, 149
CviAII CATG 2 cut(s) 190, 312
DdeI CTNAG 1 cut(s) 246
DpnI GATC 1 cut(s) 41
DpnII GATC 1 cut(s) 39
EaeI YGGCCR 2 cut(s) 496, 515
EagI CGGCCG 1 cut(s) 515
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
EciI GGCGGA 1 cut(s) 30
EclXI CGGCCG 1 cut(s) 515
Eco105I TACGTA 1 cut(s) 492
Eco130I CCWWGG 3 cut(s) 280, 367, 415
Eco147I AGGCCT 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 24
Eco47I GGWCC 2 cut(s) 406, 506
Eco52I CGGCCG 1 cut(s) 515
Eco88I CYCGRG 1 cut(s) 561
EcoRII CCWGG 1 cut(s) 393
EcoT14I CCWWGG 3 cut(s) 280, 367, 415
ErhI CCWWGG 3 cut(s) 280, 367, 415
FaeI CATG 2 cut(s) 193, 315
FaiI YATR 6 cut(s) 44, 191, 313, 353, 446, 495
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FatI CATG 2 cut(s) 189, 311
FbaI TGATCA 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 412, 515
FokI GGATG 1 cut(s) 537
Fsp4HI GCNGC 2 cut(s) 412, 515
FspBI CTAG 3 cut(s) 281, 368, 437
FspI TGCGCA 1 cut(s) 92
GlaI GCGC 1 cut(s) 92
GluI GCNGC 2 cut(s) 412, 515
GsaI CCCAGC 1 cut(s) 362
GsuI CTGGAG 2 cut(s) 77, 416
HaeIII GGCC 5 cut(s) 279, 366, 420, 498, 517
HapII CCGG 2 cut(s) 12, 518
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 93
Hin1II CATG 2 cut(s) 193, 315
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 4 cut(s) 80, 224, 254, 433
HpaII CCGG 2 cut(s) 12, 518
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 5 cut(s) 51, 139, 157, 406, 509
Hpy188I TCNGA 2 cut(s) 203, 266
Hpy188III TCNNGA 4 cut(s) 235, 437, 482, 563
Hpy8I GTNNAC 5 cut(s) 51, 139, 157, 406, 509
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 4 cut(s) 98, 161, 319, 427
HpyCH4IV ACGT 2 cut(s) 141, 491
HpyCH4V TGCA 2 cut(s) 275, 469
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 417, 523
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 2 cut(s) 141, 491
Hsp92II CATG 2 cut(s) 193, 315
HspAI GCGC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 58, 293, 396
MaeI CTAG 3 cut(s) 281, 368, 437
MaeII ACGT 2 cut(s) 141, 491
MaeIII GTNAC 1 cut(s) 7
MalI GATC 1 cut(s) 41
MboI GATC 1 cut(s) 39
MboII GAAGA 2 cut(s) 39, 155
MhlI GDGCHC 2 cut(s) 63, 444
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 2 cut(s) 117, 206
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 1 cut(s) 438
MnlI CCTC 5 cut(s) 124, 328, 337, 431, 447
Mox20I TGGCCA 1 cut(s) 498
MscI TGGCCA 1 cut(s) 498
MseI TTAA 1 cut(s) 105
Msp20I TGGCCA 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 2 cut(s) 12, 518
MspR9I CCNGG 2 cut(s) 395, 519
MvaI CCWGG 1 cut(s) 395
MwoI GCNNNNNNNGC 3 cut(s) 67, 417, 523
NciI CCSGG 1 cut(s) 519
NdeII GATC 1 cut(s) 39
NlaIII CATG 2 cut(s) 193, 315
NlaIV GGNNCC 1 cut(s) 200
NmuCI GTSAC 1 cut(s) 7
NsbI TGCGCA 1 cut(s) 92
NspI RCATGY 1 cut(s) 193
PaeR7I CTCGAG 1 cut(s) 561
PceI AGGCCT 1 cut(s) 279
PciI ACATGT 1 cut(s) 189
PfeI GAWTC 3 cut(s) 224, 254, 433
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 2 cut(s) 413, 516
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
Ppu21I YACGTR 1 cut(s) 492
PscI ACATGT 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 393
PspFI CCCAGC 1 cut(s) 358
PspGI CCWGG 1 cut(s) 393
PspN4I GGNNCC 1 cut(s) 200
PspPI GGNCC 2 cut(s) 406, 506
PsrI GAACNNNNNNTAC 2 cut(s) 176, 208
PvuII CAGCTG 1 cut(s) 358
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 2 cut(s) 412, 515
Sau3AI GATC 1 cut(s) 39
Sau96I GGNCC 2 cut(s) 406, 506
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 395, 519
SduI GDGCHC 2 cut(s) 63, 444
SetI ASST 8 cut(s) 144, 156, 339, 360, 393, 458, 490, 494
Sfr274I CTCGAG 1 cut(s) 561
SgrAI CRCCGGYG 1 cut(s) 11
SinI GGWCC 2 cut(s) 406, 506
SlaI CTCGAG 1 cut(s) 561
SmlI CTYRAG 2 cut(s) 480, 561
SmoI CTYRAG 2 cut(s) 480, 561
SnaBI TACGTA 1 cut(s) 492
Sse9I AATT 2 cut(s) 117, 206
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 5 cut(s) 15, 409, 412, 504, 514
SspMI CTAG 3 cut(s) 281, 368, 437
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 393, 517
StyI CCWWGG 3 cut(s) 280, 367, 415
TaaI ACNGT 4 cut(s) 98, 161, 319, 427
TaiI ACGT 2 cut(s) 144, 494
TaqI TCGA 2 cut(s) 452, 562
TasI AATT 2 cut(s) 117, 206
TauI GCSGC 2 cut(s) 414, 517
TfiI GAWTC 3 cut(s) 224, 254, 433
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 1 cut(s) 516
TseFI GTSAC 1 cut(s) 7
Tsp45I GTSAC 1 cut(s) 7
TspDTI ATGAA 2 cut(s) 237, 539
TspRI CASTG 1 cut(s) 516
VpaK11BI GGWCC 2 cut(s) 406, 506
XapI RAATTY 1 cut(s) 206
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 561
XmaJI CCTAGG 2 cut(s) 280, 367
XspI CTAG 3 cut(s) 281, 368, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.