FvH4_3g00860

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000587
Physical Location & Seq
Forward (+)
438844 .. 448226
9383 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00860.t2

Sequence Viewer

Length: 1539 bp
ATGGAGCACAACAGGATTGGTGTTGATCCAGAACTTCATGAGAAACCAAACACATTCTGGACACAGTGCCATATATGTTCAGCGAAGTTTCAATACGACCGAGGGTTTGTGAATCGGGTGATCCGTTGTCAAAGATGCTATAACATATTTGATGCTCGTGAATTGGAAGGTGTTCATCAAGAATCTCTCAGGAATCAATTTCCTAATCATAAAGAGCTTCCAAAACAGTGTCCTCAGTCACAAGAACCCCCAAATACATTCTGGACACAGTGCCCTTCTTGTTCTGCAAAATTTCGATACTGCAGGGACACTCTAGGTCTGTTGCTTAGTTGTCGAATCTGCCGGAAAGCATTTGAGCCTCATGAATTGAAGAAAGATGTTCATCCAGCTGCTGATTTTAGTGGGGCTTCCAACTGTGAAAAGAGAGATGTCTTGCATGGGGTCGCACTGGGGAAACATGGTGTTGAAACGTTAGAATCTGATTCAATCCAGTCCAAATGTACAGGATCTTCGAGACATATGAACAAAAAAATAGGGAATTCTGAATCTGGTTACTGTTTGAAGTGTGAAAGAAGAGCTGGCAGTGATCCTGAACTCAGTGGAGGACACTGTAAGAAATATCGAAGCAAAAGGCAGAATCCTTCATCGATTTTATATGATGATGATGACTTTGTGCGACCCCTCAAAAGTTTGAGGAAGGGTCAATTTTCTGGTGCTGCTAAGAAGAAGAGGAATATTGCAGCCGCTGGTGTGCACAAGAAAGGGGCACGAAAAAATGTTGGATTCCCTGTGGGTAAAAATTTGCAAAACAAGAAAACAGAAGATTTTGAGGAAATTGAGGTTCCTGATCCTCAATTTCATAAGTTTGTAGTTGATGCAGATAGGTTAGCAAGTCTTTGTGAAGCTAATCAAATATGGGCTCTTTACGATCCAGCAGATGGGATGCCGAGATGGTATGTTTTTGTCGAGAAGGTCTTAACCACTGAATTTAAGCTGAGAATCAGATGGCTGGATGCCGATCCAGATGACCATGGTGAGATTAATTGGTCTAAAAAGCAGTTACCAGTTGCTTGTGGTAAGTTCAGACTTCGAGAGACTGAAGAAATTACAGATCACCTTAGTTTTTCTCATAAGATGCAGTACACAAAGGGGAGTCGTACAACCTCTGTTTTGGTATATCCTAGGAGGGGAGAAACTTGGGCCATCTACCAGAATTGGGATATTGGATGGAGTTCTGAGCCGGAAACTTACAAATATGAATTTGTTGAAGTTCTGTCAGATTTTGTGGAAGGTGTTGGAATTGCAGTTACTTATTTAGGCAAAGTAAAAGGATTTGTCAGTTTATTTCAGCAAACTGAGCAGCAGGGGTTTCAAGTACCGCCTGCTGAGCTATATAGATTTTCTCATCGCATTCCCTCTTTCAAGATGACTGGTCATGAAGGATGTGGTGTTCCCTCAGGATCCTTTGAGCTAGATCCTGCTGCTTTGCCCGGCGATGTTTTGGTTCGTGACGTTGATGATATGGATATAGATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

513

Amino Acids

58.43

Weight (kDa)

8.02

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 277 - 480 4.1e-72 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 938
AciI CCGC 2 cut(s) 744, 1379
AclI AACGTT 1 cut(s) 470
AcsI RAATTY 5 cut(s) 290, 538, 799, 986, 1259
AcuI CTGAAG 1 cut(s) 1119
AfaI GTAC 4 cut(s) 502, 1142, 1159, 1377
AfiI CCNNNNNNNGG 3 cut(s) 938, 1187, 1216
AgsI TTSAA 8 cut(s) 92, 370, 467, 486, 562, 1268, 1373, 1423
AloI GAACNNNNNNTCC 2 cut(s) 1434, 1466
AluBI AGCT 7 cut(s) 217, 389, 578, 905, 994, 1390, 1471
AluI AGCT 7 cut(s) 217, 389, 578, 905, 994, 1390, 1471
Alw21I GWGCWC 2 cut(s) 9, 756
Alw26I GTCTC 2 cut(s) 508, 1088
Alw44I GTGCAC 1 cut(s) 752
AlwNI CAGNNNCTG 2 cut(s) 392, 746
AoxI GGCC 1 cut(s) 1200
ApaLI GTGCAC 1 cut(s) 752
ApeKI GCWGC 5 cut(s) 389, 716, 740, 1360, 1481
ApoI RAATTY 5 cut(s) 290, 538, 799, 986, 1259
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AseI ATTAAT 1 cut(s) 1041
Asp700I GAANNNNTTC 1 cut(s) 171
AspA2I CCTAGG 1 cut(s) 1181
AspS9I GGNCC 1 cut(s) 1200
AsuC2I CCSGG 1 cut(s) 1491
AsuHPI GGTGA 3 cut(s) 130, 1046, 1106
AvrII CCTAGG 1 cut(s) 1181
AxyI CCTNAGG 1 cut(s) 1456
BaeGI GKGCMC 3 cut(s) 275, 756, 769
BamHI GGATCC 1 cut(s) 1460
BanII GRGCYC 1 cut(s) 922
BarI GAAGNNNNNNTAC 4 cut(s) 77, 109, 493, 525
BauI CACGAG 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 9, 756
BbvI GCAGC 5 cut(s) 376, 703, 752, 1372, 1468
BccI CCATC 5 cut(s) 932, 945, 999, 1211, 1221
BcnI CCSGG 1 cut(s) 1491
BcoDI GTCTC 2 cut(s) 508, 1088
BfaI CTAG 3 cut(s) 314, 1182, 1472
BfmI CTRYAG 1 cut(s) 301
BisI GCNGC 6 cut(s) 390, 717, 741, 744, 1361, 1482
BlnI CCTAGG 1 cut(s) 1181
BlpI GCTNAGC 1 cut(s) 1386
BlsI GCNGC 6 cut(s) 391, 718, 742, 745, 1362, 1483
Bme1390I CCNGG 1 cut(s) 1491
BmgT120I GGNCC 1 cut(s) 1200
BmiI GGNNCC 2 cut(s) 843, 1462
BmrFI CCNGG 1 cut(s) 1491
BmrI ACTGGG 1 cut(s) 458
BmsI GCATC 6 cut(s) 125, 142, 865, 933, 1003, 1125
BmuI ACTGGG 1 cut(s) 458
Bpu1102I GCTNAGC 1 cut(s) 1386
BpuMI CCSGG 1 cut(s) 1491
Bsa29I ATCGAT 1 cut(s) 647
BsaBI GATNNNNATC 2 cut(s) 381, 1017
BsaJI CCNNGG 3 cut(s) 100, 1030, 1181
Bsc4I CCNNNNNNNGG 3 cut(s) 938, 1187, 1216
Bse1I ACTGG 4 cut(s) 453, 490, 1064, 1435
Bse21I CCTNAGG 1 cut(s) 1456
Bse8I GATNNNNATC 2 cut(s) 381, 1017
BseCI ATCGAT 1 cut(s) 647
BseDI CCNNGG 3 cut(s) 100, 1030, 1181
BseGI GGATG 5 cut(s) 382, 948, 1018, 1232, 1448
BseJI GATNNNNATC 2 cut(s) 381, 1017
BseLI CCNNNNNNNGG 3 cut(s) 938, 1187, 1216
BseMII CTCAG 8 cut(s) 202, 248, 610, 986, 1227, 1347, 1377, 1470
BseNI ACTGG 4 cut(s) 453, 490, 1064, 1435
BseSI GKGCMC 3 cut(s) 275, 756, 769
BseXI GCAGC 5 cut(s) 376, 703, 752, 1372, 1468
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 1 cut(s) 1202
BshVI ATCGAT 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 2 cut(s) 9, 756
BsiSI CCGG 3 cut(s) 343, 1241, 1491
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 3 cut(s) 938, 1187, 1216
BsmAI GTCTC 2 cut(s) 508, 1088
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 1 cut(s) 1410
BsnI GGCC 1 cut(s) 1202
Bsp1286I GDGCHC 5 cut(s) 9, 275, 756, 769, 922
Bsp1407I TGTACA 1 cut(s) 500
Bsp1720I GCTNAGC 1 cut(s) 1386
Bsp19I CCATGG 1 cut(s) 1030
BspACI CCGC 2 cut(s) 744, 1379
BspANI GGCC 1 cut(s) 1202
BspCNI CTCAG 8 cut(s) 201, 247, 609, 987, 1228, 1348, 1378, 1469
BspDI ATCGAT 1 cut(s) 647
BspHI TCATGA 3 cut(s) 37, 361, 1435
BspLI GGNNCC 2 cut(s) 843, 1462
BspMAI CTGCAG 1 cut(s) 305
BspQI GCTCTTC 1 cut(s) 568
BsrGI TGTACA 1 cut(s) 500
BsrI ACTGG 4 cut(s) 453, 490, 1064, 1435
BssECI CCNNGG 3 cut(s) 100, 1030, 1181
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 2 cut(s) 1030, 1181
Bst2BI CACGAG 1 cut(s) 156
Bst4CI ACNGT 6 cut(s) 66, 228, 270, 416, 557, 611
Bst6I CTCTTC 2 cut(s) 568, 722
BstAUI TGTACA 1 cut(s) 500
BstC8I GCNNGC 2 cut(s) 580, 1383
BstDSI CCRYGG 1 cut(s) 1030
BstF5I GGATG 5 cut(s) 382, 948, 1018, 1232, 1448
BstMAI GTCTC 2 cut(s) 508, 1088
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 2 cut(s) 1357, 1387
BstSCI CCNGG 1 cut(s) 1489
BstSFI CTRYAG 1 cut(s) 301
BstSLI GKGCMC 3 cut(s) 275, 756, 769
BstV1I GCAGC 5 cut(s) 376, 703, 752, 1372, 1468
BstX2I RGATCY 3 cut(s) 506, 1460, 1474
BstYI RGATCY 3 cut(s) 506, 1460, 1474
Bsu15I ATCGAT 1 cut(s) 647
Bsu36I CCTNAGG 1 cut(s) 1456
BsuRI GGCC 1 cut(s) 1202
BsuTUI ATCGAT 1 cut(s) 647
BtgI CCRYGG 1 cut(s) 1030
BtgZI GCGATG 2 cut(s) 1391, 1509
BtsCI GGATG 5 cut(s) 382, 948, 1018, 1232, 1448
BtsI GCAGTG 1 cut(s) 589
BtsIMutI CAGTG 8 cut(s) 71, 233, 275, 446, 589, 604, 607, 981
Cac8I GCNNGC 2 cut(s) 580, 1383
CaiI CAGNNNCTG 2 cut(s) 392, 746
CciI TCATGA 3 cut(s) 37, 361, 1435
Cfr13I GGNCC 1 cut(s) 1200
ClaI ATCGAT 1 cut(s) 647
Csp6I GTAC 4 cut(s) 501, 1141, 1158, 1376
CviAII CATG 6 cut(s) 38, 362, 437, 458, 1031, 1436
CviQI GTAC 4 cut(s) 501, 1141, 1158, 1376
Eam1104I CTCTTC 2 cut(s) 568, 722
EarI CTCTTC 2 cut(s) 568, 722
Eco130I CCWWGG 2 cut(s) 1030, 1181
Eco24I GRGCYC 1 cut(s) 922
Eco57I CTGAAG 1 cut(s) 1119
Eco81I CCTNAGG 1 cut(s) 1456
EcoRI GAATTC 1 cut(s) 538
EcoT14I CCWWGG 2 cut(s) 1030, 1181
EcoT38I GRGCYC 1 cut(s) 922
ErhI CCWWGG 2 cut(s) 1030, 1181
FaeI CATG 6 cut(s) 41, 365, 440, 461, 1034, 1439
FaqI GGGAC 1 cut(s) 320
FatI CATG 6 cut(s) 37, 361, 436, 457, 1030, 1435
FauNDI CATATG 1 cut(s) 519
Fnu4HI GCNGC 6 cut(s) 390, 717, 741, 744, 1361, 1482
FokI GGATG 5 cut(s) 369, 955, 1025, 1239, 1455
FriOI GRGCYC 1 cut(s) 922
Fsp4HI GCNGC 6 cut(s) 390, 717, 741, 744, 1361, 1482
FspBI CTAG 3 cut(s) 314, 1182, 1472
GluI GCNGC 6 cut(s) 390, 717, 741, 744, 1361, 1482
HaeIII GGCC 1 cut(s) 1202
HapII CCGG 3 cut(s) 343, 1241, 1491
Hin1II CATG 6 cut(s) 41, 365, 440, 461, 1034, 1439
HpaII CCGG 3 cut(s) 343, 1241, 1491
HphI GGTGA 3 cut(s) 130, 1046, 1106
Hpy166II GTNNAC 2 cut(s) 754, 1143
Hpy188I TCNGA 6 cut(s) 481, 544, 1004, 1085, 1237, 1279
Hpy8I GTNNAC 2 cut(s) 754, 1143
HpyAV CCTTC 7 cut(s) 161, 285, 651, 691, 964, 1283, 1433
HpyCH4III ACNGT 6 cut(s) 66, 228, 270, 416, 557, 611
HpyCH4IV ACGT 2 cut(s) 470, 1512
HpyCH4V TGCA 9 cut(s) 287, 303, 436, 740, 754, 805, 878, 1138, 1304
HpyF10VI GCNNNNNNNGC 2 cut(s) 1357, 1387
HpySE526I ACGT 2 cut(s) 470, 1512
Hsp92II CATG 6 cut(s) 41, 365, 440, 461, 1034, 1439
LguI GCTCTTC 1 cut(s) 568
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 5 cut(s) 376, 703, 752, 1372, 1468
LweI GCATC 6 cut(s) 125, 142, 865, 933, 1003, 1125
MaeI CTAG 3 cut(s) 314, 1182, 1472
MaeII ACGT 2 cut(s) 470, 1512
MaeIII GTNAC 5 cut(s) 237, 551, 1059, 1306, 1508
MboII GAAGA 7 cut(s) 382, 501, 585, 736, 739, 833, 1112
MflI RGATCY 3 cut(s) 506, 1460, 1474
MhlI GDGCHC 5 cut(s) 9, 275, 756, 769, 922
MlyI GAGTC 1 cut(s) 1162
MmeI TCCRAC 3 cut(s) 435, 760, 1276
MroXI GAANNNNTTC 1 cut(s) 171
MseI TTAA 3 cut(s) 977, 990, 1041
MspA1I CMGCKG 2 cut(s) 389, 746
MspI CCGG 3 cut(s) 343, 1241, 1491
MspR9I CCNGG 1 cut(s) 1491
Mva1269I GAATGC 1 cut(s) 1410
MwoI GCNNNNNNNGC 2 cut(s) 1357, 1387
NciI CCSGG 1 cut(s) 1491
NcoI CCATGG 1 cut(s) 1030
NdeI CATATG 1 cut(s) 519
NlaIII CATG 6 cut(s) 41, 365, 440, 461, 1034, 1439
NlaIV GGNNCC 2 cut(s) 843, 1462
NmeAIII GCCGAG 1 cut(s) 972
NmuCI GTSAC 2 cut(s) 237, 1508
PagI TCATGA 3 cut(s) 37, 361, 1435
PciSI GCTCTTC 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PctI GAATGC 1 cut(s) 1410
PdmI GAANNNNTTC 1 cut(s) 171
PflMI CCANNNNNTGG 1 cut(s) 938
PkrI GCNGC 6 cut(s) 391, 718, 742, 745, 1362, 1483
PleI GAGTC 1 cut(s) 1161
PpsI GAGTC 1 cut(s) 1161
PshBI ATTAAT 1 cut(s) 1041
Psp1406I AACGTT 1 cut(s) 470
PspN4I GGNNCC 2 cut(s) 843, 1462
PspPI GGNCC 1 cut(s) 1200
PstI CTGCAG 1 cut(s) 305
PstNI CAGNNNCTG 2 cut(s) 392, 746
PsuI RGATCY 3 cut(s) 506, 1460, 1474
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 4 cut(s) 502, 1142, 1159, 1377
RsaNI GTAC 4 cut(s) 501, 1141, 1158, 1376
SapI GCTCTTC 1 cut(s) 568
SaqAI TTAA 3 cut(s) 977, 990, 1041
SatI GCNGC 6 cut(s) 390, 717, 741, 744, 1361, 1482
Sau96I GGNCC 1 cut(s) 1200
SchI GAGTC 1 cut(s) 1162
ScrFI CCNGG 1 cut(s) 1491
SduI GDGCHC 5 cut(s) 9, 275, 756, 769, 922
SfaNI GCATC 6 cut(s) 125, 142, 865, 933, 1003, 1125
SfcI CTRYAG 1 cut(s) 301
SsiI CCGC 2 cut(s) 744, 1379
SspI AATATT 1 cut(s) 736
SspMI CTAG 3 cut(s) 314, 1182, 1472
StyD4I CCNGG 1 cut(s) 1489
StyI CCWWGG 2 cut(s) 1030, 1181
TaaI ACNGT 6 cut(s) 66, 228, 270, 416, 557, 611
TaiI ACGT 2 cut(s) 473, 1515
TaqI TCGA 7 cut(s) 295, 334, 512, 622, 647, 966, 1090
TaqII GACCGA 1 cut(s) 114
TatI WGTACW 2 cut(s) 500, 1140
TauI GCSGC 1 cut(s) 746
Tru1I TTAA 3 cut(s) 977, 990, 1041
Tru9I TTAA 3 cut(s) 977, 990, 1041
TscAI CASTG 8 cut(s) 71, 233, 275, 453, 589, 604, 614, 988
TseFI GTSAC 2 cut(s) 237, 1508
TseI GCWGC 5 cut(s) 389, 716, 740, 1360, 1481
Tsp45I GTSAC 2 cut(s) 237, 1508
TspDTI ATGAA 9 cut(s) 26, 164, 371, 378, 536, 633, 848, 1272, 1452
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 8 cut(s) 71, 233, 275, 453, 589, 604, 614, 988
Van91I CCANNNNNTGG 1 cut(s) 938
VneI GTGCAC 1 cut(s) 752
VspI ATTAAT 1 cut(s) 1041
XapI RAATTY 5 cut(s) 290, 538, 799, 986, 1259
XcmI CCANNNNNNNNNTGG 2 cut(s) 54, 258
XmaJI CCTAGG 1 cut(s) 1181
XmnI GAANNNNTTC 1 cut(s) 171
XspI CTAG 3 cut(s) 314, 1182, 1472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.