Rh5DG010700

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000587
Physical Location & Seq
Forward (+)
742073 .. 742918
846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG010700.1

Sequence Viewer

Length: 846 bp
ATGCTAGATAAAGAGGAAAGCAACTTCAAAGCTGATGTCAGTTCTGCAGCAAATGTAGCCACTCTGGAGAGCATTGAGGTTCCTGATCCTCAATTTCATAAGTTTGTAGTTGATGCAGACATGTTGGCAAATCTTTGTCAAGCTAATCAAACATGGGCTCTTTATGATACAGCAGATGGCATGCCCAGATTCTATGCTATTGTCAAGAAGGTCCTCACCCCTGGATTTAAGCTGAAAGTCAGCTGGCTGGACGCGGATCCAGATGACCAAGGTGAGATTGATTGGTGTGAAAAGGAGTTGCCAGTTGCTTGCGGTAAGTTCAGACTTGGAGACTCTGCAGAAATTACGGATCACCTTATGTTTTCTCATCAGATGCACGCCACAACAGGGAGTCTTACAGGCTCTGTTTTGGTATATCCTAGGAAGGGAGAAACTTGGGCCATCTACCAGAATTGGGATATTGGGTGGAGTTCTGAGCCGGAAAAGCACGTATCTTACAAATATGAATTTGTTGAAGTCTTGTCAGATTTTGTGGAAGGTGATGGAATTGGAGTTTCTTATTTAGGCAAAGTAAAAGGATTTGTTAGTTTATTTCAGCAAACCGAGCAGCATGGGTTTCCAGTACCACCTGATGAGCTTTATAGATTTTCTCATCGAATTCCCTCTTTCAAGATGACTGGTCATGAAGGATATGGTGTTCCCTCAGGATCCTTTGAGCTAGATCCTGCTTCTTTGCCCTCCAGTATTTTGATTCATGATGTACGTGATTTGGAAATCGATAACAGAAGGAAGAATACTGAACCTAGTGATTTGTCTGGTGAATTTGTAGAAAGGTTGGTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

281

Amino Acids

31.56

Weight (kDa)

4.74

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 23 - 229 3e-75 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 254
AciI CCGC 2 cut(s) 254, 312
AclWI GGATC 7 cut(s) 80, 251, 264, 357, 702, 715, 716
AcsI RAATTY 3 cut(s) 506, 657, 821
AfaI GTAC 2 cut(s) 624, 762
AfiI CCNNNNNNNGG 3 cut(s) 387, 425, 454
AflIII ACRYGT 1 cut(s) 120
AgsI TTSAA 3 cut(s) 28, 515, 670
AjnI CCWGG 1 cut(s) 220
AloI GAACNNNNNNTCC 2 cut(s) 681, 713
AluBI AGCT 6 cut(s) 32, 143, 232, 243, 637, 718
AluI AGCT 6 cut(s) 32, 143, 232, 243, 637, 718
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 7 cut(s) 80, 251, 264, 357, 702, 715, 716
AlwNI CAGNNNCTG 1 cut(s) 404
AoxI GGCC 1 cut(s) 438
ApeKI GCWGC 2 cut(s) 47, 607
ApoI RAATTY 3 cut(s) 506, 657, 821
ArsI GACNNNNNNTTYG 2 cut(s) 21, 53
AspA2I CCTAGG 1 cut(s) 419
AspS9I GGNCC 2 cut(s) 211, 438
AsuHPI GGTGA 5 cut(s) 208, 284, 344, 551, 830
AvaII GGWCC 1 cut(s) 211
AvrII CCTAGG 1 cut(s) 419
AxyI CCTNAGG 1 cut(s) 703
BamHI GGATCC 2 cut(s) 256, 707
BanII GRGCYC 1 cut(s) 160
BarI GAAGNNNNNNTAC 2 cut(s) 778, 810
BbvI GCAGC 2 cut(s) 59, 619
BccI CCATC 3 cut(s) 170, 449, 536
BciT130I CCWGG 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 4 cut(s) 5, 420, 719, 804
BfmI CTRYAG 2 cut(s) 45, 336
BisI GCNGC 2 cut(s) 48, 608
BlnI CCTAGG 1 cut(s) 419
BlsI GCNGC 2 cut(s) 49, 609
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 211, 438
BmiI GGNNCC 3 cut(s) 81, 258, 709
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 103, 363
BpmI CTGGAG 2 cut(s) 86, 724
Bsa29I ATCGAT 1 cut(s) 777
BsaAI YACGTR 2 cut(s) 490, 764
BsaJI CCNNGG 3 cut(s) 220, 268, 419
Bsc4I CCNNNNNNNGG 3 cut(s) 387, 425, 454
Bse1I ACTGG 4 cut(s) 302, 620, 682, 741
Bse21I CCTNAGG 1 cut(s) 703
BseBI CCWGG 1 cut(s) 222
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 3 cut(s) 220, 268, 419
BseLI CCNNNNNNNGG 3 cut(s) 387, 425, 454
BseMII CTCAG 2 cut(s) 465, 717
BseNI ACTGG 4 cut(s) 302, 620, 682, 741
BseXI GCAGC 2 cut(s) 59, 619
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 1 cut(s) 440
BshVI ATCGAT 1 cut(s) 777
BsiSI CCGG 1 cut(s) 479
BslI CCNNNNNNNGG 3 cut(s) 387, 425, 454
BsmAI GTCTC 1 cut(s) 324
BsnI GGCC 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 160
Bsp143I GATC 5 cut(s) 85, 256, 349, 707, 721
BspACI CCGC 2 cut(s) 254, 312
BspANI GGCC 1 cut(s) 440
BspCNI CTCAG 2 cut(s) 466, 716
BspDI ATCGAT 1 cut(s) 777
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 2 cut(s) 682, 754
BspLI GGNNCC 3 cut(s) 81, 258, 709
BspMAI CTGCAG 2 cut(s) 49, 340
BspPI GGATC 7 cut(s) 80, 251, 264, 357, 702, 715, 716
BsrI ACTGG 4 cut(s) 302, 620, 682, 741
BssECI CCNNGG 3 cut(s) 220, 268, 419
BssMI GATC 5 cut(s) 85, 256, 349, 707, 721
BssT1I CCWWGG 2 cut(s) 268, 419
Bst2UI CCWGG 1 cut(s) 222
BstBAI YACGTR 2 cut(s) 490, 764
BstC8I GCNNGC 4 cut(s) 182, 245, 310, 378
BstDEI CTNAG 2 cut(s) 474, 703
BstFNI CGCG 1 cut(s) 254
BstKTI GATC 5 cut(s) 88, 259, 352, 710, 724
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 5 cut(s) 85, 256, 349, 707, 721
BstMWI GCNNNNNNNGC 3 cut(s) 56, 484, 604
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 2 cut(s) 124, 184
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 2 cut(s) 45, 336
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 2 cut(s) 59, 619
BstX2I RGATCY 3 cut(s) 256, 707, 721
BstYI RGATCY 3 cut(s) 256, 707, 721
Bsu15I ATCGAT 1 cut(s) 777
Bsu36I CCTNAGG 1 cut(s) 703
BsuRI GGCC 1 cut(s) 440
BsuTUI ATCGAT 1 cut(s) 777
Cac8I GCNNGC 4 cut(s) 182, 245, 310, 378
CaiI CAGNNNCTG 1 cut(s) 404
CciI TCATGA 2 cut(s) 682, 754
Cfr13I GGNCC 2 cut(s) 211, 438
ClaI ATCGAT 1 cut(s) 777
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 623, 761
CviAII CATG 6 cut(s) 121, 153, 181, 611, 683, 755
CviQI GTAC 2 cut(s) 623, 761
DdeI CTNAG 2 cut(s) 474, 703
DpnI GATC 5 cut(s) 87, 258, 351, 709, 723
DpnII GATC 5 cut(s) 85, 256, 349, 707, 721
Eco130I CCWWGG 2 cut(s) 268, 419
Eco24I GRGCYC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 211
Eco81I CCTNAGG 1 cut(s) 703
EcoO109I RGGNCCY 1 cut(s) 211
EcoRI GAATTC 1 cut(s) 657
EcoRII CCWGG 1 cut(s) 220
EcoT14I CCWWGG 2 cut(s) 268, 419
EcoT38I GRGCYC 1 cut(s) 160
ErhI CCWWGG 2 cut(s) 268, 419
FaeI CATG 6 cut(s) 124, 156, 184, 614, 686, 758
FatI CATG 6 cut(s) 120, 152, 180, 610, 682, 754
Fnu4HI GCNGC 2 cut(s) 48, 608
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 2 cut(s) 48, 608
FspBI CTAG 4 cut(s) 5, 420, 719, 804
GluI GCNGC 2 cut(s) 48, 608
GsuI CTGGAG 2 cut(s) 86, 724
HaeIII GGCC 1 cut(s) 440
HapII CCGG 1 cut(s) 479
HgaI GACGC 1 cut(s) 260
Hin1II CATG 6 cut(s) 124, 156, 184, 614, 686, 758
HinfI GANTC 4 cut(s) 189, 332, 391, 751
HpaII CCGG 1 cut(s) 479
HphI GGTGA 5 cut(s) 208, 284, 344, 551, 830
Hpy188I TCNGA 4 cut(s) 323, 372, 475, 526
Hpy188III TCNNGA 8 cut(s) 65, 83, 205, 260, 670, 683, 705, 755
HpyAV CCTTC 5 cut(s) 202, 418, 530, 680, 780
HpyCH4IV ACGT 2 cut(s) 489, 763
HpyCH4V TGCA 4 cut(s) 47, 116, 338, 376
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 484, 604
HpyF3I CTNAG 2 cut(s) 474, 703
HpySE526I ACGT 2 cut(s) 489, 763
Hsp92II CATG 6 cut(s) 124, 156, 184, 614, 686, 758
Kzo9I GATC 5 cut(s) 85, 256, 349, 707, 721
Lsp1109I GCAGC 2 cut(s) 59, 619
LweI GCATC 2 cut(s) 103, 363
MaeI CTAG 4 cut(s) 5, 420, 719, 804
MaeII ACGT 2 cut(s) 489, 763
MalI GATC 5 cut(s) 87, 258, 351, 709, 723
MboI GATC 5 cut(s) 85, 256, 349, 707, 721
MboII GAAGA 1 cut(s) 802
MflI RGATCY 3 cut(s) 256, 707, 721
MhlI GDGCHC 1 cut(s) 160
MluCI AATT 7 cut(s) 92, 342, 451, 506, 546, 657, 821
MlyI GAGTC 2 cut(s) 326, 400
MnlI CCTC 7 cut(s) 7, 70, 99, 224, 673, 712, 748
MseI TTAA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 243
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 3 cut(s) 56, 484, 604
NdeII GATC 5 cut(s) 85, 256, 349, 707, 721
NlaIII CATG 6 cut(s) 124, 156, 184, 614, 686, 758
NlaIV GGNNCC 3 cut(s) 81, 258, 709
NspI RCATGY 2 cut(s) 124, 184
PaeI GCATGC 1 cut(s) 184
PagI TCATGA 2 cut(s) 682, 754
PciI ACATGT 1 cut(s) 120
PfeI GAWTC 2 cut(s) 189, 751
PkrI GCNGC 2 cut(s) 49, 609
PleI GAGTC 2 cut(s) 326, 399
PpsI GAGTC 2 cut(s) 326, 399
Ppu21I YACGTR 2 cut(s) 490, 764
PpuMI RGGWCCY 1 cut(s) 211
PscI ACATGT 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 3 cut(s) 81, 258, 709
PspPI GGNCC 2 cut(s) 211, 438
PspPPI RGGWCCY 1 cut(s) 211
PstI CTGCAG 2 cut(s) 49, 340
PstNI CAGNNNCTG 1 cut(s) 404
PsuI RGATCY 3 cut(s) 256, 707, 721
PvuII CAGCTG 1 cut(s) 243
RsaI GTAC 2 cut(s) 624, 762
RsaNI GTAC 2 cut(s) 623, 761
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 2 cut(s) 48, 608
Sau3AI GATC 5 cut(s) 85, 256, 349, 707, 721
Sau96I GGNCC 2 cut(s) 211, 438
SchI GAGTC 2 cut(s) 326, 400
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 1 cut(s) 160
SfaNI GCATC 2 cut(s) 103, 363
SfcI CTRYAG 2 cut(s) 45, 336
SinI GGWCC 1 cut(s) 211
SphI GCATGC 1 cut(s) 184
Sse9I AATT 7 cut(s) 92, 342, 451, 506, 546, 657, 821
SsiI CCGC 2 cut(s) 254, 312
SspMI CTAG 4 cut(s) 5, 420, 719, 804
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 2 cut(s) 268, 419
TaiI ACGT 2 cut(s) 492, 766
TaqI TCGA 2 cut(s) 655, 777
TasI AATT 7 cut(s) 92, 342, 451, 506, 546, 657, 821
TfiI GAWTC 2 cut(s) 189, 751
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TseI GCWGC 2 cut(s) 47, 607
TspDTI ATGAA 4 cut(s) 86, 519, 699, 743
TspGWI ACGGA 1 cut(s) 362
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 3 cut(s) 506, 657, 821
XceI RCATGY 2 cut(s) 124, 184
XmaJI CCTAGG 1 cut(s) 419
XspI CTAG 4 cut(s) 5, 420, 719, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.