Rroxscaffold_6G00430260

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000587
Physical Location & Seq
Forward (+)
50412327 .. 50418070
5744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00430260.1

Sequence Viewer

Length: 2097 bp
ATGGAGTGCAACAAAGAGGAGGCGGTCAGAGCAATGCAACTTTCAGAAACAAAGATGCAAAACAAAGATTATATAGGAGCAATGAAGATGGCACAAAAGGCTCAGAGACTCTGCCCTGATCTCGAAAACATTTCTCGGTTACTTACGGTATGTGAAGTTCACTGTACAGCAGAAAACAAGCTAGGTGGATCCGAAATGGATTGGTACGGAATTCTTCAGATTCAACAATTTGATAATGATGTCACCATCAAGAAACAGTATAGAAAATTGGCACTTTTGCTTCATCCTGATAAGAATAAGTTTGCTGGTTCGGAGGCTGCTTTTAAGCTGATTGGGGAAGCAAACAGGGTGCTGTCGGACAAACAAAAACGTTCTATATATGACATTAAGTATAGAACTTTAGCAAGACCTGGTGGTCCAAAAACAACAGCTCATCAGTCAAATATTGATATCTTTGCTGCAACCAATTCTCGGAATAATCCTCAGTCACCTGTGCAACCAAACACATTCTGGACACAGTGCTACTATTGTTTCTCAAAGTTTCAATACTATAGAGACTTTCTGGGTCGGTTGCTCCGTTGTCAAAGATGCCGGAAAGCATTTGAAGCTCATGAATTTCGGGAAGGTGTGCATCCAGAATCTTTCAGAAATGACTTTCCGAATCATAAAGAGCCGCCAAGTCAGGGTCCTTCAAACATAGCCTCACAAAGTAATGGAGGAACTGAAAAAGGAAATTTCACAAGATTTCAGAATGGAGATGCAGCCTTCAAACCTGCGTCAAAGGTAGGAACTTCTGCTGACTTGAGTGGGGCTTCTGACGCTGAAAAGAAAGATATTAGGCGTGGGGTTGAAGTGGGGAAGCAAGGTGTTGAAACATCTAAATTTGGTCCAGTGAAGTCCAAAGATACAATAACTTCTAGAAATATGAACAAAGAAAGAGAGAATTCAACTTTTGAATTTAGAGAAAGTGTGAAGACTGGAAATAGAGCTACAGCTGATACTGAACGTACTGCTAAAAAGAAGAATGATAGTGTTTCTGAACTCGGTGGAGGCCGTCACCCTAGGAGATCTCCAAGGAACAAGCATAATCTTTCATCCCTCCTAGATGACAAGGATGATGATGATTTTGTGAGTCCCCCATTCAAAAGGTTGAGGAAGGGCCAATTGTGTAGTGCTGCTGAGGAGGGAAATAATGCAACTGTTGGTGGACATAAGAAAAATACAAGAGAAAAAGTAAGTTTTCCTCTAGAAGAAAATTTGCCAAAGAAGAAGAGTAATACTGAGGAATTTGAGTCGACGGTAAAGGGAGCCGCCATGTCAGATCATGATGAAAGCAACTTCAAAGCTGATTTCAGTCCTGTAGCTAATGTAGATGTTGCATCCACTCCGGGAGCCGTTGAAGTTCCTGATCCTCAATTCCATAAGTTTGTGCTTGATGAAGATAGTTTACCAAGTCTTTTTGAAGCCAATCAAATTTGGGCTCTTTATGATCCAGCAGATGACATGCCCAGATACTATGCTTTTGTCAAGAAGGTCCTCACCCCTGGATTTAAGCTGAAAATCAGATGGCTAGAGGCCAATCCAGATGACCAATGTGAGATTAATTGGTCCGACAAGGAGTTGCCAGTTGCTTGTGGCAAGTTCAAACTTGGGAAAACTCAAGAAATTAAAGGTCATCTTATGTTTTCTCATCAGATGCACTACAGAAAAGGAAGTGATAAAAAGTCTATTCTGGTATATCCTAGGAAGGGAGAAACTTGGGCCATGTACCAGAATTGGGATATTGGATGGAGTTCTGAGCCGGAAAAGCACATGCCCTACAAATATGAATTTGTTGAAGTCTTGTCTGATTTTGTCGAAGCTTTTGGAATTGGAGTTCGTTATTTAGGCAAAGTGAAAGGATTTGTCAGTTTATTTCAGCCAACTGAGCAGCACGGGGTTGTCATGTTTCAAGTACCAACTCATGAGCTATACAGATTTTCTCATCAGATTCCCTCTTTCAAGATGACCGGTTGTGAAGGATCTGGTGTTCCCCAGGATCCTTTGAGCTTGATCCTGCTTCTTTGCCCACCAGTATTTTTGATTCAAGTGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

698

Amino Acids

79.04

Weight (kDa)

8.75

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 128 4.5e-20 DnaJ domain
DUF3444 PF11926 465 - 672 7.5e-78 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 414
Acc36I ACCTGC 1 cut(s) 781
AccI GTMKAC 1 cut(s) 1295
AciI CCGC 3 cut(s) 23, 674, 1311
AclI AACGTT 1 cut(s) 370
AclWI GGATC 8 cut(s) 183, 196, 1403, 1484, 2029, 2033, 2046, 2047
AcuI CTGAAG 1 cut(s) 200
AfaI GTAC 5 cut(s) 166, 206, 1009, 1769, 1956
AfiI CCNNNNNNNGG 4 cut(s) 471, 683, 1748, 1777
AgeI ACCGGT 1 cut(s) 2009
AjnI CCWGG 3 cut(s) 409, 1543, 2034
AloI GAACNNNNNNTCC 4 cut(s) 1860, 1892, 2013, 2045
Alw26I GTCTC 2 cut(s) 100, 549
AlwI GGATC 8 cut(s) 183, 196, 1403, 1484, 2029, 2033, 2046, 2047
AoxI GGCC 4 cut(s) 1051, 1159, 1575, 1761
ApeKI GCWGC 5 cut(s) 317, 458, 761, 1175, 1930
AseI ATTAAT 1 cut(s) 1602
AsiGI ACCGGT 1 cut(s) 2009
AspA2I CCTAGG 2 cut(s) 1061, 1742
AspS9I GGNCC 7 cut(s) 416, 686, 887, 1159, 1534, 1608, 1761
AsuC2I CCSGG 1 cut(s) 1389
AsuHPI GGTGA 4 cut(s) 235, 480, 1049, 1531
AvaII GGWCC 5 cut(s) 416, 686, 887, 1534, 1608
AvrII CCTAGG 2 cut(s) 1061, 1742
BamHI GGATCC 2 cut(s) 188, 2038
BanII GRGCYC 1 cut(s) 1483
BarI GAAGNNNNNNTAC 2 cut(s) 1431, 1463
BbsI GAAGAC 1 cut(s) 980
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 5 cut(s) 304, 445, 773, 1162, 1942
BccI CCATC 4 cut(s) 82, 254, 1560, 1782
BceAI ACGGC 2 cut(s) 1038, 1379
BciT130I CCWGG 3 cut(s) 411, 1545, 2036
BcnI CCSGG 1 cut(s) 1389
BcoDI GTCTC 2 cut(s) 100, 549
BfaI CTAG 8 cut(s) 182, 918, 1062, 1103, 1247, 1571, 1743, 2095
BfmI CTRYAG 4 cut(s) 550, 990, 1359, 1702
BfuAI ACCTGC 1 cut(s) 781
BglII AGATCT 1 cut(s) 1067
BisI GCNGC 7 cut(s) 318, 459, 674, 762, 1176, 1311, 1931
BlnI CCTAGG 2 cut(s) 1061, 1742
BlsI GCNGC 7 cut(s) 319, 460, 675, 763, 1177, 1312, 1932
Bme1390I CCNGG 4 cut(s) 411, 1389, 1545, 2036
Bme18I GGWCC 5 cut(s) 416, 686, 887, 1534, 1608
BmgT120I GGNCC 7 cut(s) 416, 686, 887, 1159, 1534, 1608, 1761
BmiI GGNNCC 5 cut(s) 190, 687, 1309, 1393, 2040
BmrFI CCNGG 4 cut(s) 411, 1389, 1545, 2036
BmsI GCATC 6 cut(s) 45, 578, 640, 748, 1388, 1686
BpiI GAAGAC 1 cut(s) 980
Bpu10I CCTNAGC 1 cut(s) 1179
BpuEI CTTGAG 2 cut(s) 823, 1644
BpuMI CCSGG 1 cut(s) 1389
BsaJI CCNNGG 5 cut(s) 1061, 1073, 1543, 1742, 2034
BsaWI WCCGGW 1 cut(s) 2009
Bsc4I CCNNNNNNNGG 4 cut(s) 471, 683, 1748, 1777
Bse118I RCCGGY 1 cut(s) 2009
Bse1I ACTGG 4 cut(s) 890, 982, 1625, 2072
Bse3DI GCAATG 2 cut(s) 39, 87
BseBI CCWGG 3 cut(s) 411, 1545, 2036
BseDI CCNNGG 5 cut(s) 1061, 1073, 1543, 1742, 2034
BseGI GGATG 6 cut(s) 283, 631, 1094, 1120, 1379, 1793
BseLI CCNNNNNNNGG 4 cut(s) 471, 683, 1748, 1777
BseMI GCAATG 2 cut(s) 39, 87
BseMII CTCAG 6 cut(s) 116, 497, 1170, 1272, 1788, 1917
BseNI ACTGG 4 cut(s) 890, 982, 1625, 2072
BseRI GAGGAG 2 cut(s) 32, 1196
BseXI GCAGC 5 cut(s) 304, 445, 773, 1162, 1942
BshFI GGCC 4 cut(s) 1053, 1161, 1577, 1763
BshTI ACCGGT 1 cut(s) 2009
BsiSI CCGG 4 cut(s) 592, 1388, 1802, 2010
BslFI GGGAC 1 cut(s) 1119
BslI CCNNNNNNNGG 4 cut(s) 471, 683, 1748, 1777
BsmAI GTCTC 2 cut(s) 100, 549
BsmFI GGGAC 1 cut(s) 1119
BsnI GGCC 4 cut(s) 1053, 1161, 1577, 1763
Bsp1286I GDGCHC 1 cut(s) 1483
Bsp1407I TGTACA 1 cut(s) 164
BspACI CCGC 3 cut(s) 23, 674, 1311
BspANI GGCC 4 cut(s) 1053, 1161, 1577, 1763
BspCNI CTCAG 6 cut(s) 115, 496, 1171, 1273, 1789, 1918
BspHI TCATGA 3 cut(s) 610, 1324, 1963
BspLI GGNNCC 5 cut(s) 190, 687, 1309, 1393, 2040
BspMI ACCTGC 1 cut(s) 781
BspPI GGATC 8 cut(s) 183, 196, 1403, 1484, 2029, 2033, 2046, 2047
BsrDI GCAATG 2 cut(s) 39, 87
BsrFI RCCGGY 1 cut(s) 2009
BsrGI TGTACA 1 cut(s) 164
BsrI ACTGG 4 cut(s) 890, 982, 1625, 2072
BssAI RCCGGY 1 cut(s) 2009
BssECI CCNNGG 5 cut(s) 1061, 1073, 1543, 1742, 2034
BssT1I CCWWGG 3 cut(s) 1061, 1073, 1742
Bst2UI CCWGG 3 cut(s) 411, 1545, 2036
Bst4CI ACNGT 6 cut(s) 148, 164, 258, 519, 1201, 1300
Bst6I CTCTTC 1 cut(s) 1265
BstAUI TGTACA 1 cut(s) 164
BstDEI CTNAG 6 cut(s) 102, 483, 1179, 1281, 1797, 1926
BstF5I GGATG 6 cut(s) 283, 631, 1094, 1120, 1379, 1793
BstMAI GTCTC 2 cut(s) 100, 549
BstMWI GCNNNNNNNGC 6 cut(s) 29, 98, 605, 818, 1807, 1927
BstNI CCWGG 3 cut(s) 411, 1545, 2036
BstNSI RCATGY 2 cut(s) 1507, 1816
BstSCI CCNGG 4 cut(s) 409, 1387, 1543, 2034
BstSFI CTRYAG 4 cut(s) 550, 990, 1359, 1702
BstV1I GCAGC 5 cut(s) 304, 445, 773, 1162, 1942
BstV2I GAAGAC 1 cut(s) 980
BstX2I RGATCY 4 cut(s) 188, 1067, 2021, 2038
BstYI RGATCY 4 cut(s) 188, 1067, 2021, 2038
BsuRI GGCC 4 cut(s) 1053, 1161, 1577, 1763
BtsCI GGATG 6 cut(s) 283, 631, 1094, 1120, 1379, 1793
BtsIMutI CAGTG 3 cut(s) 160, 524, 897
BveI ACCTGC 1 cut(s) 781
CciI TCATGA 3 cut(s) 610, 1324, 1963
Cfr10I RCCGGY 1 cut(s) 2009
Cfr13I GGNCC 7 cut(s) 416, 686, 887, 1159, 1534, 1608, 1761
CseI GACGC 2 cut(s) 765, 827
CsiI ACCWGGT 1 cut(s) 409
Csp6I GTAC 5 cut(s) 165, 205, 1008, 1768, 1955
CspAI ACCGGT 1 cut(s) 2009
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 8 cut(s) 611, 1315, 1325, 1504, 1765, 1813, 1945, 1964
CviQI GTAC 5 cut(s) 165, 205, 1008, 1768, 1955
DdeI CTNAG 6 cut(s) 102, 483, 1179, 1281, 1797, 1926
DrdI GACNNNNNNGTC 1 cut(s) 414
DseDI GACNNNNNNGTC 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 1265
EarI CTCTTC 1 cut(s) 1265
Eco130I CCWWGG 3 cut(s) 1061, 1073, 1742
Eco24I GRGCYC 1 cut(s) 1483
Eco32I GATATC 1 cut(s) 451
Eco47I GGWCC 5 cut(s) 416, 686, 887, 1534, 1608
Eco57I CTGAAG 1 cut(s) 200
EcoO109I RGGNCCY 2 cut(s) 686, 1534
EcoRI GAATTC 2 cut(s) 210, 943
EcoRII CCWGG 3 cut(s) 409, 1543, 2034
EcoRV GATATC 1 cut(s) 451
EcoT14I CCWWGG 3 cut(s) 1061, 1073, 1742
EcoT38I GRGCYC 1 cut(s) 1483
ErhI CCWWGG 3 cut(s) 1061, 1073, 1742
FaeI CATG 8 cut(s) 614, 1318, 1328, 1507, 1768, 1816, 1948, 1967
FalI AAGNNNNNCTT 2 cut(s) 1662, 1694
FaqI GGGAC 1 cut(s) 1119
FatI CATG 8 cut(s) 610, 1314, 1324, 1503, 1764, 1812, 1944, 1963
FblI GTMKAC 1 cut(s) 1295
Fnu4HI GCNGC 7 cut(s) 318, 459, 674, 762, 1176, 1311, 1931
FokI GGATG 6 cut(s) 270, 618, 1081, 1127, 1366, 1800
FriOI GRGCYC 1 cut(s) 1483
Fsp4HI GCNGC 7 cut(s) 318, 459, 674, 762, 1176, 1311, 1931
FspBI CTAG 8 cut(s) 182, 918, 1062, 1103, 1247, 1571, 1743, 2095
GluI GCNGC 7 cut(s) 318, 459, 674, 762, 1176, 1311, 1931
HaeIII GGCC 4 cut(s) 1053, 1161, 1577, 1763
HapII CCGG 4 cut(s) 592, 1388, 1802, 2010
HgaI GACGC 2 cut(s) 765, 827
Hin1II CATG 8 cut(s) 614, 1318, 1328, 1507, 1768, 1816, 1948, 1967
HincII GTYRAC 1 cut(s) 1296
HindII GTYRAC 1 cut(s) 1296
HindIII AAGCTT 1 cut(s) 1860
HinfI GANTC 8 cut(s) 108, 220, 638, 661, 1132, 1292, 1990, 2083
HpaII CCGG 4 cut(s) 592, 1388, 1802, 2010
HphI GGTGA 4 cut(s) 235, 480, 1049, 1531
Hpy166II GTNNAC 4 cut(s) 160, 1208, 1296, 1448
Hpy8I GTNNAC 4 cut(s) 160, 1208, 1296, 1448
Hpy99I CGWCG 1 cut(s) 1300
HpyAV CCTTC 7 cut(s) 617, 699, 775, 1150, 1525, 1741, 2012
HpyCH4III ACNGT 6 cut(s) 148, 164, 258, 519, 1201, 1300
HpyCH4IV ACGT 2 cut(s) 370, 1006
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 98, 605, 818, 1807, 1927
HpyF3I CTNAG 6 cut(s) 102, 483, 1179, 1281, 1797, 1926
HpySE526I ACGT 2 cut(s) 370, 1006
Hsp92II CATG 8 cut(s) 614, 1318, 1328, 1507, 1768, 1816, 1948, 1967
LmnI GCTCC 4 cut(s) 77, 579, 1307, 1391
Lsp1109I GCAGC 5 cut(s) 304, 445, 773, 1162, 1942
LweI GCATC 6 cut(s) 45, 578, 640, 748, 1388, 1686
MabI ACCWGGT 1 cut(s) 409
MaeI CTAG 8 cut(s) 182, 918, 1062, 1103, 1247, 1571, 1743, 2095
MaeII ACGT 2 cut(s) 370, 1006
MaeIII GTNAC 4 cut(s) 138, 241, 486, 1055
MboII GAAGA 8 cut(s) 97, 206, 985, 1033, 1262, 1279, 1282, 1451
MfeI CAATTG 1 cut(s) 1163
MflI RGATCY 4 cut(s) 188, 1067, 2021, 2038
MhlI GDGCHC 1 cut(s) 1483
MlyI GAGTC 3 cut(s) 102, 1141, 1301
MmeI TCCRAC 2 cut(s) 336, 1635
MseI TTAA 5 cut(s) 324, 387, 1551, 1602, 1668
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 4 cut(s) 592, 1388, 1802, 2010
MspR9I CCNGG 4 cut(s) 411, 1389, 1545, 2036
MunI CAATTG 1 cut(s) 1163
MvaI CCWGG 3 cut(s) 411, 1545, 2036
MwoI GCNNNNNNNGC 6 cut(s) 29, 98, 605, 818, 1807, 1927
NciI CCSGG 1 cut(s) 1389
NlaIII CATG 8 cut(s) 614, 1318, 1328, 1507, 1768, 1816, 1948, 1967
NlaIV GGNNCC 5 cut(s) 190, 687, 1309, 1393, 2040
NmuCI GTSAC 3 cut(s) 241, 486, 1055
NspI RCATGY 2 cut(s) 1507, 1816
PagI TCATGA 3 cut(s) 610, 1324, 1963
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PfeI GAWTC 5 cut(s) 220, 638, 661, 1990, 2083
PfoI TCCNGGA 1 cut(s) 1387
PinAI ACCGGT 1 cut(s) 2009
PkrI GCNGC 7 cut(s) 319, 460, 675, 763, 1177, 1312, 1932
PleI GAGTC 3 cut(s) 102, 1140, 1300
PpsI GAGTC 3 cut(s) 102, 1140, 1300
PpuMI RGGWCCY 2 cut(s) 686, 1534
PshBI ATTAAT 1 cut(s) 1602
Psp1406I AACGTT 1 cut(s) 370
Psp5II RGGWCCY 2 cut(s) 686, 1534
Psp6I CCWGG 3 cut(s) 409, 1543, 2034
PspGI CCWGG 3 cut(s) 409, 1543, 2034
PspN4I GGNNCC 5 cut(s) 190, 687, 1309, 1393, 2040
PspPI GGNCC 7 cut(s) 416, 686, 887, 1159, 1534, 1608, 1761
PspPPI RGGWCCY 2 cut(s) 686, 1534
PsrI GAACNNNNNNTAC 2 cut(s) 141, 173
PsuI RGATCY 4 cut(s) 188, 1067, 2021, 2038
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 5 cut(s) 166, 206, 1009, 1769, 1956
RsaNI GTAC 5 cut(s) 165, 205, 1008, 1768, 1955
SalI GTCGAC 1 cut(s) 1294
SaqAI TTAA 5 cut(s) 324, 387, 1551, 1602, 1668
SatI GCNGC 7 cut(s) 318, 459, 674, 762, 1176, 1311, 1931
Sau96I GGNCC 7 cut(s) 416, 686, 887, 1159, 1534, 1608, 1761
SchI GAGTC 3 cut(s) 102, 1141, 1301
ScrFI CCNGG 4 cut(s) 411, 1389, 1545, 2036
SduI GDGCHC 1 cut(s) 1483
SexAI ACCWGGT 1 cut(s) 409
SfaNI GCATC 6 cut(s) 45, 578, 640, 748, 1388, 1686
SfcI CTRYAG 4 cut(s) 550, 990, 1359, 1702
SinI GGWCC 5 cut(s) 416, 686, 887, 1534, 1608
SmlI CTYRAG 2 cut(s) 802, 1659
SmoI CTYRAG 2 cut(s) 802, 1659
SsiI CCGC 3 cut(s) 23, 674, 1311
SspI AATATT 1 cut(s) 445
SspMI CTAG 8 cut(s) 182, 918, 1062, 1103, 1247, 1571, 1743, 2095
StyD4I CCNGG 4 cut(s) 409, 1387, 1543, 2034
StyI CCWWGG 3 cut(s) 1061, 1073, 1742
TaaI ACNGT 6 cut(s) 148, 164, 258, 519, 1201, 1300
TaiI ACGT 2 cut(s) 373, 1009
TaqI TCGA 3 cut(s) 123, 1295, 1857
TatI WGTACW 1 cut(s) 164
TauI GCSGC 2 cut(s) 676, 1313
TfiI GAWTC 5 cut(s) 220, 638, 661, 1990, 2083
Tru1I TTAA 5 cut(s) 324, 387, 1551, 1602, 1668
Tru9I TTAA 5 cut(s) 324, 387, 1551, 1602, 1668
TscAI CASTG 3 cut(s) 167, 524, 897
TseFI GTSAC 3 cut(s) 241, 486, 1055
TseI GCWGC 5 cut(s) 317, 458, 761, 1175, 1930
Tsp45I GTSAC 3 cut(s) 241, 486, 1055
TspDTI ATGAA 8 cut(s) 98, 272, 627, 941, 1083, 1344, 1452, 1842
TspGWI ACGGA 2 cut(s) 222, 566
TspRI CASTG 3 cut(s) 167, 524, 897
VpaK11BI GGWCC 5 cut(s) 416, 686, 887, 1534, 1608
VspI ATTAAT 1 cut(s) 1602
XbaI TCTAGA 2 cut(s) 917, 1246
XceI RCATGY 2 cut(s) 1507, 1816
XcmI CCANNNNNNNNNTGG 1 cut(s) 507
XmaJI CCTAGG 2 cut(s) 1061, 1742
XmiI GTMKAC 1 cut(s) 1295
XspI CTAG 8 cut(s) 182, 918, 1062, 1103, 1247, 1571, 1743, 2095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.