Rmu_co8484983.1_g000001

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8484983.1
OG0000587
Physical Location & Seq
Forward (+)
152 .. 2340
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8484983.1_g000001.1.cds

Sequence Viewer

Length: 2189 bp
atggagtgcaacaaagaggaggcggtcagagcaatgcaactttcagaaacaaagatgcaaaacaaagattatataggagcaatgaagacagcacaaaaggctcagagactcttccctgatctcgaaaacatttctcggttacttacggtatgtgaagttcactgtacagcagaaaacaagctaggtggatccgaaatggattggtacagaattcttcagattcaacaatttgataatgatgtcaccatcaagaaacagtatagaaaattggcacttttgcttcatcctgataagaataagtttgctggttcggaggctgcttttaagctgattggggaagcaaacagggtgctgtcggacaaacaaaaacgttctatatatgacattaagtatagaactttagcaagacatggtggatcaaaaacaacagctcatcagtcaaatattgatttctttgctgcaaccaattctcagaataatcctcagtcacctgtgcaaccaaacacattctggacacagtgctactattgtttctcaaagtttcaatactatagagactttctgggtcggttgctccgttgtcaaagatgccggaaagcatttgaagctcatgaatttcgggaaggtgtgcatccagaatctttcagaaatgactttccaaattataaagagccgacaagtcagggtccttcaaacgtagcctcacaaagtaatggaggaactgaaaaaggaaatttcacaagatttcagaatggagatgcagccttcaaacctgtgtcaaaggtaggaacttctgctgacttgagtggggcttctgatgctgaaaagaaagatatttggcgtggggttgaagtggggaagcaaggtgttgaaacatctaaatttggtccagtgaagtccaaagatacaataacttctagaaatatgaacaaagaaagagagaattcaacttttgaatttagagaaagtttgaagactggaaatagagctacagctgatactgaacgtgctgctaaaaagaagaatgatagtgtttctgaactcggtggaggccgtcaccctaggagatctccaaggaacaagcataatctttcatccctcctagatgacaatgatgatgatgattttgtgagtcccccattcaaaaggttgaggaagggccaattgtttagtgctgctgaggagggaaataatgcaactgttggtggacataagaaaaatacaagagaaaaagtaagatttcctctagaagaaaatttgccaaagaagaagagtaaaactgaggaatttgagttaacggtaaagggaaccgccatgtcagatcatgatgaaagcaacttcaaaggtgatttcagtcctgtagctaatgtagatgttgcatccactccgggagccattgaagttcctgatcctcaattccataagtttgtggttgatgaagatagtttgccaagtctttttgaagccaatcaaatatgggctctttatgatccagcagatggcatgcccagatattatgcttttgtcaagaaggtcctcacccctggatttaagctgaaaatcagatggctggaggccaatccagatgaccaaggtgagattgattggtccgagatggagttgccagttgcttgtggcaagttcaaacttgggaaaactcaagaaattaaagatcatcatatgttttctcatcagatgcactacagaaaaggaagtgataaaaagtctattctggtatatcctaggaagggagaaacttgggccatgtaccagaattgggatattggatggagttctgagccggaaaagcacacgccctacaaatatgaatttgttgaagtcttgtctgattttgtcgaagcttttggaattggagttcgttatttaggcaaagtgaaaggatttgtcagtttatttcagccaactgagcagcacggggttgtcatgtttcaagtaccaactcatgagctatacagattttctcatcagattccctctttcaagatgactggttgtgaaggatctggtgttcccccaggttcctttgagcttgatcctgcttctttgcccaccagtatttttgattcaagtgatctaggtaacttggaaatggatgacagaagcaagaaaactgaaactgatggtacgtcttgtgaaagggttggttctgtaactgcatgcacaggtat
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

729

Amino Acids

82.45

Weight (kDa)

8.09

Isoelectric Point (pI)

38.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AccB7I CCANNNNNTGG 1 cut(s) 1499
AciI CCGC 2 cut(s) 23, 1311
AclI AACGTT 1 cut(s) 370
AclWI GGATC 7 cut(s) 183, 196, 424, 1403, 1484, 2029, 2048
AcsI RAATTY 9 cut(s) 210, 614, 733, 881, 943, 956, 1255, 1286, 1829
AcuI CTGAAG 1 cut(s) 200
AfaI GTAC 5 cut(s) 166, 206, 1769, 1956, 2146
AfiI CCNNNNNNNGG 3 cut(s) 1499, 1748, 1777
AjnI CCWGG 2 cut(s) 1543, 2035
AloI GAACNNNNNNTCC 4 cut(s) 1860, 1892, 2013, 2045
Alw26I GTCTC 2 cut(s) 100, 549
AlwI GGATC 7 cut(s) 183, 196, 424, 1403, 1484, 2029, 2048
AoxI GGCC 4 cut(s) 1051, 1159, 1575, 1761
ApeKI GCWGC 6 cut(s) 317, 458, 761, 1010, 1175, 1930
ApoI RAATTY 9 cut(s) 210, 614, 733, 881, 943, 956, 1255, 1286, 1829
AspA2I CCTAGG 2 cut(s) 1061, 1742
AspS9I GGNCC 6 cut(s) 686, 887, 1159, 1534, 1608, 1761
AsuC2I CCSGG 1 cut(s) 1389
AsuHPI GGTGA 6 cut(s) 235, 480, 1049, 1358, 1531, 1607
AvaII GGWCC 4 cut(s) 686, 887, 1534, 1608
AvrII CCTAGG 2 cut(s) 1061, 1742
BamHI GGATCC 1 cut(s) 188
BanII GRGCYC 1 cut(s) 1483
BbsI GAAGAC 2 cut(s) 92, 980
BbvCI CCTCAGC 1 cut(s) 1179
BbvI GCAGC 6 cut(s) 304, 445, 773, 997, 1162, 1942
BccI CCATC 6 cut(s) 254, 1493, 1560, 1609, 1782, 2135
BceAI ACGGC 1 cut(s) 1038
BciT130I CCWGG 2 cut(s) 1545, 2037
BcnI CCSGG 1 cut(s) 1389
BcoDI GTCTC 2 cut(s) 100, 549
BfaI CTAG 7 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096
BfmI CTRYAG 4 cut(s) 550, 990, 1359, 1702
BglII AGATCT 1 cut(s) 1067
BisI GCNGC 6 cut(s) 318, 459, 762, 1011, 1176, 1931
BlnI CCTAGG 2 cut(s) 1061, 1742
BlsI GCNGC 6 cut(s) 319, 460, 763, 1012, 1177, 1932
Bme1390I CCNGG 3 cut(s) 1389, 1545, 2037
Bme18I GGWCC 4 cut(s) 686, 887, 1534, 1608
BmgT120I GGNCC 6 cut(s) 686, 887, 1159, 1534, 1608, 1761
BmiI GGNNCC 5 cut(s) 190, 687, 1309, 1393, 2041
BmrFI CCNGG 3 cut(s) 1389, 1545, 2037
BmsI GCATC 7 cut(s) 45, 578, 640, 748, 808, 1388, 1686
BpiI GAAGAC 2 cut(s) 92, 980
BpmI CTGGAG 1 cut(s) 1592
Bpu10I CCTNAGC 1 cut(s) 1179
BpuEI CTTGAG 2 cut(s) 823, 1644
BpuMI CCSGG 1 cut(s) 1389
BsaJI CCNNGG 6 cut(s) 1061, 1073, 1543, 1591, 1742, 2035
Bsc4I CCNNNNNNNGG 3 cut(s) 1499, 1748, 1777
Bse1I ACTGG 5 cut(s) 890, 982, 1625, 2014, 2073
Bse3DI GCAATG 2 cut(s) 39, 87
BseBI CCWGG 2 cut(s) 1545, 2037
BseDI CCNNGG 6 cut(s) 1061, 1073, 1543, 1591, 1742, 2035
BseGI GGATG 6 cut(s) 283, 631, 1094, 1379, 1793, 2119
BseLI CCNNNNNNNGG 3 cut(s) 1499, 1748, 1777
BseMI GCAATG 2 cut(s) 39, 87
BseMII CTCAG 7 cut(s) 116, 485, 497, 1170, 1272, 1788, 1917
BseNI ACTGG 5 cut(s) 890, 982, 1625, 2014, 2073
BseRI GAGGAG 2 cut(s) 32, 1196
BseXI GCAGC 6 cut(s) 304, 445, 773, 997, 1162, 1942
BshFI GGCC 4 cut(s) 1053, 1161, 1577, 1763
BsiSI CCGG 3 cut(s) 592, 1388, 1802
BslFI GGGAC 1 cut(s) 1119
BslI CCNNNNNNNGG 3 cut(s) 1499, 1748, 1777
BsmAI GTCTC 2 cut(s) 100, 549
BsmFI GGGAC 1 cut(s) 1119
BsnI GGCC 4 cut(s) 1053, 1161, 1577, 1763
Bsp1286I GDGCHC 1 cut(s) 1483
Bsp1407I TGTACA 1 cut(s) 164
BspACI CCGC 2 cut(s) 23, 1311
BspANI GGCC 4 cut(s) 1053, 1161, 1577, 1763
BspCNI CTCAG 7 cut(s) 115, 484, 496, 1171, 1273, 1789, 1918
BspHI TCATGA 3 cut(s) 610, 1324, 1963
BspLI GGNNCC 5 cut(s) 190, 687, 1309, 1393, 2041
BspPI GGATC 7 cut(s) 183, 196, 424, 1403, 1484, 2029, 2048
BsrDI GCAATG 2 cut(s) 39, 87
BsrGI TGTACA 1 cut(s) 164
BsrI ACTGG 5 cut(s) 890, 982, 1625, 2014, 2073
BssECI CCNNGG 6 cut(s) 1061, 1073, 1543, 1591, 1742, 2035
BssT1I CCWWGG 4 cut(s) 1061, 1073, 1591, 1742
Bst2UI CCWGG 2 cut(s) 1545, 2037
Bst4CI ACNGT 6 cut(s) 148, 164, 258, 519, 1201, 1300
Bst6I CTCTTC 2 cut(s) 116, 1265
BstAUI TGTACA 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 1505, 2179
BstDEI CTNAG 7 cut(s) 102, 471, 483, 1179, 1281, 1797, 1926
BstF5I GGATG 6 cut(s) 283, 631, 1094, 1379, 1793, 2119
BstMAI GTCTC 2 cut(s) 100, 549
BstMWI GCNNNNNNNGC 6 cut(s) 29, 98, 605, 818, 1807, 1927
BstNI CCWGG 2 cut(s) 1545, 2037
BstNSI RCATGY 2 cut(s) 1507, 2181
BstSCI CCNGG 3 cut(s) 1387, 1543, 2035
BstSFI CTRYAG 4 cut(s) 550, 990, 1359, 1702
BstV1I GCAGC 6 cut(s) 304, 445, 773, 997, 1162, 1942
BstV2I GAAGAC 2 cut(s) 92, 980
BstX2I RGATCY 3 cut(s) 188, 1067, 2021
BstYI RGATCY 3 cut(s) 188, 1067, 2021
BsuRI GGCC 4 cut(s) 1053, 1161, 1577, 1763
BtsCI GGATG 6 cut(s) 283, 631, 1094, 1379, 1793, 2119
BtsIMutI CAGTG 3 cut(s) 160, 524, 897
Cac8I GCNNGC 2 cut(s) 1505, 2179
CciI TCATGA 3 cut(s) 610, 1324, 1963
Cfr13I GGNCC 6 cut(s) 686, 887, 1159, 1534, 1608, 1761
Csp6I GTAC 5 cut(s) 165, 205, 1768, 1955, 2145
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
CviAII CATG 9 cut(s) 410, 611, 1315, 1325, 1504, 1765, 1945, 1964, 2178
CviQI GTAC 5 cut(s) 165, 205, 1768, 1955, 2145
DdeI CTNAG 7 cut(s) 102, 471, 483, 1179, 1281, 1797, 1926
Eam1104I CTCTTC 2 cut(s) 116, 1265
EarI CTCTTC 2 cut(s) 116, 1265
Eco130I CCWWGG 4 cut(s) 1061, 1073, 1591, 1742
Eco24I GRGCYC 1 cut(s) 1483
Eco47I GGWCC 4 cut(s) 686, 887, 1534, 1608
Eco57I CTGAAG 1 cut(s) 200
EcoO109I RGGNCCY 2 cut(s) 686, 1534
EcoRI GAATTC 2 cut(s) 210, 943
EcoRII CCWGG 2 cut(s) 1543, 2035
EcoT14I CCWWGG 4 cut(s) 1061, 1073, 1591, 1742
EcoT38I GRGCYC 1 cut(s) 1483
ErhI CCWWGG 4 cut(s) 1061, 1073, 1591, 1742
FaeI CATG 9 cut(s) 413, 614, 1318, 1328, 1507, 1768, 1948, 1967, 2181
FaqI GGGAC 1 cut(s) 1119
FatI CATG 9 cut(s) 409, 610, 1314, 1324, 1503, 1764, 1944, 1963, 2177
FauNDI CATATG 1 cut(s) 1680
Fnu4HI GCNGC 6 cut(s) 318, 459, 762, 1011, 1176, 1931
FokI GGATG 6 cut(s) 270, 618, 1081, 1366, 1800, 2126
FriOI GRGCYC 1 cut(s) 1483
Fsp4HI GCNGC 6 cut(s) 318, 459, 762, 1011, 1176, 1931
FspBI CTAG 7 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096
GluI GCNGC 6 cut(s) 318, 459, 762, 1011, 1176, 1931
GsuI CTGGAG 1 cut(s) 1592
HaeIII GGCC 4 cut(s) 1053, 1161, 1577, 1763
HapII CCGG 3 cut(s) 592, 1388, 1802
Hin1II CATG 9 cut(s) 413, 614, 1318, 1328, 1507, 1768, 1948, 1967, 2181
HincII GTYRAC 1 cut(s) 1296
HindII GTYRAC 1 cut(s) 1296
HindIII AAGCTT 1 cut(s) 1860
HinfI GANTC 6 cut(s) 108, 220, 638, 1132, 1990, 2084
HpaI GTTAAC 1 cut(s) 1296
HpaII CCGG 3 cut(s) 592, 1388, 1802
HphI GGTGA 6 cut(s) 235, 480, 1049, 1358, 1531, 1607
Hpy166II GTNNAC 3 cut(s) 160, 1208, 1296
Hpy8I GTNNAC 3 cut(s) 160, 1208, 1296
HpyAV CCTTC 7 cut(s) 617, 699, 775, 1150, 1525, 1741, 2012
HpyCH4III ACNGT 6 cut(s) 148, 164, 258, 519, 1201, 1300
HpyCH4IV ACGT 4 cut(s) 370, 696, 1006, 2147
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 98, 605, 818, 1807, 1927
HpyF3I CTNAG 7 cut(s) 102, 471, 483, 1179, 1281, 1797, 1926
HpySE526I ACGT 4 cut(s) 370, 696, 1006, 2147
Hsp92II CATG 9 cut(s) 413, 614, 1318, 1328, 1507, 1768, 1948, 1967, 2181
KspAI GTTAAC 1 cut(s) 1296
LmnI GCTCC 3 cut(s) 77, 579, 1391
Lsp1109I GCAGC 6 cut(s) 304, 445, 773, 997, 1162, 1942
LweI GCATC 7 cut(s) 45, 578, 640, 748, 808, 1388, 1686
MaeI CTAG 7 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096
MaeII ACGT 4 cut(s) 370, 696, 1006, 2147
MaeIII GTNAC 6 cut(s) 138, 241, 486, 1055, 2099, 2170
MboII GAAGA 9 cut(s) 97, 103, 206, 985, 1033, 1262, 1279, 1282, 1451
MfeI CAATTG 1 cut(s) 1163
MflI RGATCY 3 cut(s) 188, 1067, 2021
MhlI GDGCHC 1 cut(s) 1483
MlyI GAGTC 2 cut(s) 102, 1141
MmeI TCCRAC 1 cut(s) 336
MseI TTAA 5 cut(s) 324, 387, 1295, 1551, 1668
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 3 cut(s) 592, 1388, 1802
MspR9I CCNGG 3 cut(s) 1389, 1545, 2037
MunI CAATTG 1 cut(s) 1163
MvaI CCWGG 2 cut(s) 1545, 2037
MwoI GCNNNNNNNGC 6 cut(s) 29, 98, 605, 818, 1807, 1927
NciI CCSGG 1 cut(s) 1389
NdeI CATATG 1 cut(s) 1680
NlaIII CATG 9 cut(s) 413, 614, 1318, 1328, 1507, 1768, 1948, 1967, 2181
NlaIV GGNNCC 5 cut(s) 190, 687, 1309, 1393, 2041
NmuCI GTSAC 3 cut(s) 241, 486, 1055
NspI RCATGY 2 cut(s) 1507, 2181
PaeI GCATGC 2 cut(s) 1507, 2181
PagI TCATGA 3 cut(s) 610, 1324, 1963
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PfeI GAWTC 4 cut(s) 220, 638, 1990, 2084
PflMI CCANNNNNTGG 1 cut(s) 1499
PfoI TCCNGGA 1 cut(s) 1387
PkrI GCNGC 6 cut(s) 319, 460, 763, 1012, 1177, 1932
PleI GAGTC 2 cut(s) 102, 1140
PpsI GAGTC 2 cut(s) 102, 1140
PpuMI RGGWCCY 2 cut(s) 686, 1534
PsiI TTATAA 1 cut(s) 666
Psp1406I AACGTT 1 cut(s) 370
Psp5II RGGWCCY 2 cut(s) 686, 1534
Psp6I CCWGG 2 cut(s) 1543, 2035
PspGI CCWGG 2 cut(s) 1543, 2035
PspN4I GGNNCC 5 cut(s) 190, 687, 1309, 1393, 2041
PspPI GGNCC 6 cut(s) 686, 887, 1159, 1534, 1608, 1761
PspPPI RGGWCCY 2 cut(s) 686, 1534
PsrI GAACNNNNNNTAC 2 cut(s) 141, 173
PsuI RGATCY 3 cut(s) 188, 1067, 2021
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 5 cut(s) 166, 206, 1769, 1956, 2146
RsaNI GTAC 5 cut(s) 165, 205, 1768, 1955, 2145
SaqAI TTAA 5 cut(s) 324, 387, 1295, 1551, 1668
SatI GCNGC 6 cut(s) 318, 459, 762, 1011, 1176, 1931
Sau96I GGNCC 6 cut(s) 686, 887, 1159, 1534, 1608, 1761
SchI GAGTC 2 cut(s) 102, 1141
ScrFI CCNGG 3 cut(s) 1389, 1545, 2037
SduI GDGCHC 1 cut(s) 1483
SfaNI GCATC 7 cut(s) 45, 578, 640, 748, 808, 1388, 1686
SfcI CTRYAG 4 cut(s) 550, 990, 1359, 1702
SinI GGWCC 4 cut(s) 686, 887, 1534, 1608
SmlI CTYRAG 2 cut(s) 802, 1659
SmoI CTYRAG 2 cut(s) 802, 1659
SphI GCATGC 2 cut(s) 1507, 2181
SsiI CCGC 2 cut(s) 23, 1311
SspI AATATT 1 cut(s) 445
SspMI CTAG 7 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096
StyD4I CCNGG 3 cut(s) 1387, 1543, 2035
StyI CCWWGG 4 cut(s) 1061, 1073, 1591, 1742
TaaI ACNGT 6 cut(s) 148, 164, 258, 519, 1201, 1300
TaiI ACGT 4 cut(s) 373, 699, 1009, 2150
TaqI TCGA 2 cut(s) 123, 1857
TatI WGTACW 1 cut(s) 164
TfiI GAWTC 4 cut(s) 220, 638, 1990, 2084
Tru1I TTAA 5 cut(s) 324, 387, 1295, 1551, 1668
Tru9I TTAA 5 cut(s) 324, 387, 1295, 1551, 1668
TscAI CASTG 3 cut(s) 167, 524, 897
TseFI GTSAC 3 cut(s) 241, 486, 1055
TseI GCWGC 6 cut(s) 317, 458, 761, 1010, 1175, 1930
Tsp45I GTSAC 3 cut(s) 241, 486, 1055
TspDTI ATGAA 8 cut(s) 98, 272, 627, 941, 1083, 1344, 1452, 1842
TspGWI ACGGA 1 cut(s) 566
TspRI CASTG 3 cut(s) 167, 524, 897
Van91I CCANNNNNTGG 1 cut(s) 1499
VpaK11BI GGWCC 4 cut(s) 686, 887, 1534, 1608
XapI RAATTY 9 cut(s) 210, 614, 733, 881, 943, 956, 1255, 1286, 1829
XbaI TCTAGA 2 cut(s) 917, 1246
XceI RCATGY 2 cut(s) 1507, 2181
XcmI CCANNNNNNNNNTGG 1 cut(s) 507
XmaJI CCTAGG 2 cut(s) 1061, 1742
XspI CTAG 7 cut(s) 182, 918, 1062, 1103, 1247, 1743, 2096
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.