Rh5AG010200

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000587
Physical Location & Seq
Forward (+)
722955 .. 723932
978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG010200.1

Sequence Viewer

Length: 978 bp
ATGGGGCGCAAAGGGAGCGGTGTTGATACAGAAATTCAGGAGAAACCAAGCACATTCTGGACACAGTGTCCCATTTGTTGGGCAAAACTTCAATACTACACGGAGTTTCTGGATCGGTTGCTCCGTTGTCAAATATGCCATAACACATTTCAAGCTCGTGAATTGTTAGAAGATGTTGATGCAGAAGCTCTCAGGAATCAATGTCCAAACCATAAAGAGCCTCCAAAACAGTGTCCACAGGACCCCCCAAACACATTCTGGACACAGTGCTCCTTTTGTTGCGCAAAATTTCAATACTACAGTGATACTCTGGGTCGATTGCTTTGTTGTCAAATATGTCAGAAAGCATTTGAGGCTCATGAATTGAAGAACGATGTTCATCCAGAATTTTTCAGGAATCAATTTTCGAATACTGTGGAGCCTCCAAAACAGGGTCCATCAAACTTAGCCTCAGAAAGTAGTGGTGGAAGTAAAAAGGGAAATATCACAAGATTTCAAGACGGAGATGGAACCTTCAAAAGTTTGAGGAAGGGTCAATCTTCTGGTGTCGCTGAGAAGAAGAGGAATATTGGAGCTGCTGGTGTACATAAGAAAGAGGCAAGAGAAAATGTTAGTTTCCCTCTGGATAAAAATTTTCCAAACAAGAAGAGGAAAACAGAAGAATTTGAGTTAAAAGCAAAGGAAGCCACCATGCTAGATAAAGAGGAAAGCAACTTCAAAGCTGATGTCAGTTCTGCAGCAAATGTAGCCACTCTGGAGAGCATTGAGGTTCCTGATCCTCAATTTCATAAGTTTGTAGTTGATGCAGACATGTTGGCAAATCTTTGTCAAGCTAATCAAACATGGGCTCTTTATGATACAGCAGATGGCATGCCCAGATTCTATGCTATTGTCAAGAAGGTTCTCACCCCTGGATTTAAGCTGAAAGTCAGCTGGCTGGACGCGGATCCAGATGACCAAGGTGAGATTGATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

325

Amino Acids

36.83

Weight (kDa)

6.13

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 253 - 325 6.4e-19 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 283
AccB7I CCANNNNNTGG 1 cut(s) 78
AccBSI CCGCTC 1 cut(s) 18
AccII CGCG 1 cut(s) 944
AciI CCGC 2 cut(s) 18, 944
AclWI GGATC 4 cut(s) 120, 770, 941, 954
AcsI RAATTY 5 cut(s) 33, 287, 386, 631, 662
AfaI GTAC 1 cut(s) 585
AfiI CCNNNNNNNGG 2 cut(s) 78, 431
AflIII ACRYGT 1 cut(s) 810
AgsI TTSAA 7 cut(s) 92, 152, 293, 367, 497, 517, 718
AhdI GACNNNNNGTC 1 cut(s) 66
AjnI CCWGG 1 cut(s) 910
AluBI AGCT 7 cut(s) 155, 188, 575, 722, 833, 922, 933
AluI AGCT 7 cut(s) 155, 188, 575, 722, 833, 922, 933
Alw21I GWGCWC 1 cut(s) 272
AlwI GGATC 4 cut(s) 120, 770, 941, 954
ApeKI GCWGC 2 cut(s) 575, 737
ApoI RAATTY 5 cut(s) 33, 287, 386, 631, 662
ArsI GACNNNNNNTTYG 2 cut(s) 711, 743
AspLEI GCGC 2 cut(s) 9, 284
AspS9I GGNCC 2 cut(s) 241, 434
AsuHPI GGTGA 2 cut(s) 898, 974
AsuII TTCGAA 1 cut(s) 407
AvaII GGWCC 2 cut(s) 241, 434
BamHI GGATCC 1 cut(s) 946
BanII GRGCYC 1 cut(s) 850
BauI CACGAG 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 2 cut(s) 562, 749
BccI CCATC 3 cut(s) 445, 500, 860
BciT130I CCWGG 1 cut(s) 912
BfaI CTAG 1 cut(s) 695
BfmI CTRYAG 2 cut(s) 298, 735
BisI GCNGC 2 cut(s) 576, 738
BlsI GCNGC 2 cut(s) 577, 739
Bme1390I CCNGG 1 cut(s) 912
Bme18I GGWCC 2 cut(s) 241, 434
BmeRI GACNNNNNGTC 1 cut(s) 66
BmgT120I GGNCC 2 cut(s) 241, 434
BmiI GGNNCC 6 cut(s) 243, 420, 435, 511, 771, 948
BmrFI CCNGG 1 cut(s) 912
BmsI GCATC 2 cut(s) 169, 793
BpmI CTGGAG 1 cut(s) 776
Bpu14I TTCGAA 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 2 cut(s) 910, 958
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 431
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 1 cut(s) 912
BseDI CCNNGG 2 cut(s) 910, 958
BseGI GGATG 1 cut(s) 379
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 2 cut(s) 78, 431
BseMII CTCAG 3 cut(s) 205, 465, 543
BseXI GCAGC 2 cut(s) 562, 749
Bsh1236I CGCG 1 cut(s) 944
BsiHKAI GWGCWC 1 cut(s) 272
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 78, 431
BsmFI GGGAC 1 cut(s) 54
Bsp119I TTCGAA 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 272, 850
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 3 cut(s) 112, 775, 946
BspACI CCGC 2 cut(s) 18, 944
BspCNI CTCAG 3 cut(s) 204, 464, 544
BspFNI CGCG 1 cut(s) 944
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 6 cut(s) 243, 420, 435, 511, 771, 948
BspMAI CTGCAG 1 cut(s) 739
BspPI GGATC 4 cut(s) 120, 770, 941, 954
BspT104I TTCGAA 1 cut(s) 407
BsrBI CCGCTC 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 583
BssECI CCNNGG 2 cut(s) 910, 958
BssMI GATC 3 cut(s) 112, 775, 946
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 958
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 1 cut(s) 912
Bst4CI ACNGT 5 cut(s) 66, 231, 267, 302, 415
Bst6I CTCTTC 2 cut(s) 554, 641
BstAUI TGTACA 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 407
BstC8I GCNNGC 2 cut(s) 872, 935
BstDEI CTNAG 4 cut(s) 191, 445, 451, 552
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 944
BstHHI GCGC 2 cut(s) 9, 284
BstKTI GATC 3 cut(s) 115, 778, 949
BstMBI GATC 3 cut(s) 112, 775, 946
BstMWI GCNNNNNNNGC 4 cut(s) 15, 353, 683, 746
BstNI CCWGG 1 cut(s) 912
BstNSI RCATGY 2 cut(s) 814, 874
BstSCI CCNGG 1 cut(s) 910
BstSFI CTRYAG 2 cut(s) 298, 735
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 2 cut(s) 562, 749
BstX2I RGATCY 1 cut(s) 946
BstYI RGATCY 1 cut(s) 946
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 4 cut(s) 71, 236, 272, 307
Cac8I GCNNGC 2 cut(s) 872, 935
CciI TCATGA 1 cut(s) 358
CfoI GCGC 2 cut(s) 9, 284
Cfr13I GGNCC 2 cut(s) 241, 434
CseI GACGC 1 cut(s) 950
Csp6I GTAC 1 cut(s) 584
CviAII CATG 5 cut(s) 359, 691, 811, 843, 871
CviQI GTAC 1 cut(s) 584
DdeI CTNAG 4 cut(s) 191, 445, 451, 552
DpnI GATC 3 cut(s) 114, 777, 948
DpnII GATC 3 cut(s) 112, 775, 946
DriI GACNNNNNGTC 1 cut(s) 66
Eam1104I CTCTTC 2 cut(s) 554, 641
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 2 cut(s) 554, 641
Eco130I CCWWGG 1 cut(s) 958
Eco24I GRGCYC 1 cut(s) 850
Eco47I GGWCC 2 cut(s) 241, 434
EcoO109I RGGNCCY 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 910
EcoT14I CCWWGG 1 cut(s) 958
EcoT38I GRGCYC 1 cut(s) 850
ErhI CCWWGG 1 cut(s) 958
FaeI CATG 5 cut(s) 362, 694, 814, 846, 874
FaqI GGGAC 1 cut(s) 54
FatI CATG 5 cut(s) 358, 690, 810, 842, 870
Fnu4HI GCNGC 2 cut(s) 576, 738
FokI GGATG 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 850
Fsp4HI GCNGC 2 cut(s) 576, 738
FspBI CTAG 1 cut(s) 695
FspI TGCGCA 1 cut(s) 283
GlaI GCGC 2 cut(s) 8, 283
GluI GCNGC 2 cut(s) 576, 738
GsuI CTGGAG 1 cut(s) 776
HgaI GACGC 1 cut(s) 950
HhaI GCGC 2 cut(s) 9, 284
Hin1II CATG 5 cut(s) 362, 694, 814, 846, 874
Hin6I GCGC 2 cut(s) 7, 282
HinP1I GCGC 2 cut(s) 7, 282
HinfI GANTC 3 cut(s) 196, 397, 879
HphI GGTGA 2 cut(s) 898, 974
Hpy166II GTNNAC 2 cut(s) 236, 584
Hpy188I TCNGA 2 cut(s) 342, 454
Hpy8I GTNNAC 2 cut(s) 236, 584
HpyAV CCTTC 3 cut(s) 523, 523, 892
HpyCH4III ACNGT 5 cut(s) 66, 231, 267, 302, 415
HpyCH4V TGCA 3 cut(s) 182, 737, 806
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 353, 683, 746
HpyF3I CTNAG 4 cut(s) 191, 445, 451, 552
Hsp92II CATG 5 cut(s) 362, 694, 814, 846, 874
HspAI GCGC 2 cut(s) 7, 282
Kzo9I GATC 3 cut(s) 112, 775, 946
LmnI GCTCC 5 cut(s) 15, 126, 275, 418, 572
Lsp1109I GCAGC 2 cut(s) 562, 749
LweI GCATC 2 cut(s) 169, 793
MaeI CTAG 1 cut(s) 695
MalI GATC 3 cut(s) 114, 777, 948
MbiI CCGCTC 1 cut(s) 18
MboI GATC 3 cut(s) 112, 775, 946
MboII GAAGA 7 cut(s) 182, 379, 531, 568, 571, 658, 671
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 2 cut(s) 272, 850
MluCI AATT 9 cut(s) 33, 161, 287, 362, 386, 401, 631, 662, 782
MseI TTAA 2 cut(s) 671, 918
MspA1I CMGCKG 1 cut(s) 933
MspR9I CCNGG 1 cut(s) 912
MvaI CCWGG 1 cut(s) 912
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 4 cut(s) 15, 353, 683, 746
NdeII GATC 3 cut(s) 112, 775, 946
NlaIII CATG 5 cut(s) 362, 694, 814, 846, 874
NlaIV GGNNCC 6 cut(s) 243, 420, 435, 511, 771, 948
NsbI TGCGCA 1 cut(s) 283
NspI RCATGY 2 cut(s) 814, 874
NspV TTCGAA 1 cut(s) 407
PaeI GCATGC 1 cut(s) 874
PagI TCATGA 1 cut(s) 358
PciI ACATGT 1 cut(s) 810
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PfeI GAWTC 3 cut(s) 196, 397, 879
PflMI CCANNNNNTGG 1 cut(s) 78
PkrI GCNGC 2 cut(s) 577, 739
PpuMI RGGWCCY 1 cut(s) 241
PscI ACATGT 1 cut(s) 810
Psp5II RGGWCCY 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 910
PspGI CCWGG 1 cut(s) 910
PspN4I GGNNCC 6 cut(s) 243, 420, 435, 511, 771, 948
PspPI GGNCC 2 cut(s) 241, 434
PspPPI RGGWCCY 1 cut(s) 241
PstI CTGCAG 1 cut(s) 739
PsuI RGATCY 1 cut(s) 946
PvuII CAGCTG 1 cut(s) 933
RsaI GTAC 1 cut(s) 585
RsaNI GTAC 1 cut(s) 584
SaqAI TTAA 2 cut(s) 671, 918
SatI GCNGC 2 cut(s) 576, 738
Sau3AI GATC 3 cut(s) 112, 775, 946
Sau96I GGNCC 2 cut(s) 241, 434
ScrFI CCNGG 1 cut(s) 912
SduI GDGCHC 2 cut(s) 272, 850
SfaNI GCATC 2 cut(s) 169, 793
SfcI CTRYAG 2 cut(s) 298, 735
SfuI TTCGAA 1 cut(s) 407
SinI GGWCC 2 cut(s) 241, 434
SphI GCATGC 1 cut(s) 874
Sse9I AATT 9 cut(s) 33, 161, 287, 362, 386, 401, 631, 662, 782
SsiI CCGC 2 cut(s) 18, 944
SspI AATATT 1 cut(s) 568
SspMI CTAG 1 cut(s) 695
StyD4I CCNGG 1 cut(s) 910
StyI CCWWGG 1 cut(s) 958
TaaI ACNGT 5 cut(s) 66, 231, 267, 302, 415
TaqI TCGA 2 cut(s) 316, 407
TasI AATT 9 cut(s) 33, 161, 287, 362, 386, 401, 631, 662, 782
TatI WGTACW 1 cut(s) 583
TfiI GAWTC 3 cut(s) 196, 397, 879
Tru1I TTAA 2 cut(s) 671, 918
Tru9I TTAA 2 cut(s) 671, 918
TscAI CASTG 4 cut(s) 71, 236, 272, 307
TseI GCWGC 2 cut(s) 575, 737
TspDTI ATGAA 3 cut(s) 368, 375, 776
TspGWI ACGGA 3 cut(s) 113, 116, 516
TspRI CASTG 4 cut(s) 71, 236, 272, 307
Van91I CCANNNNNTGG 1 cut(s) 78
VpaK11BI GGWCC 2 cut(s) 241, 434
XapI RAATTY 5 cut(s) 33, 287, 386, 631, 662
XceI RCATGY 2 cut(s) 814, 874
XcmI CCANNNNNNNNNTGG 2 cut(s) 54, 255
XspI CTAG 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.