FvH4_6g04720

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000587
Physical Location & Seq
Forward (+)
2594702 .. 2595346
645 bp
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UTR
Exon/CDS
Intron
FvH4_6g04720.t1

Sequence Viewer

Length: 645 bp
ATGATAGGTCACCTTACTGCGGCTTCTGTGGATGGACCTAATAAGGCACTACAAAAATTAAGCTTCCCACTAGAAGAATGTTTGCCTAGCAAAAAGAGTAAAACTGGGGAACCTGTGCCAGATAATGATGAAACCAACTTCAAAGCTAATGTTAATCCTGTGCCAAATGTTGGTGTTGCATCCACACCAGAACTCATTGAGGTTCCTAATCCTCAATTTCATAAGTTTGTGCTTAATGACAAAACGTTGGTAGGTACTTTTAAAGCTAATCAAACATGGGCTCTTTTTGATGGTGAAGCAGATGGCATGCCCAGATACTATGCTTTTGTCAAGGAAGTATTAAACACCCCGGGATTTAAGCTGAAAATCGTCTTGCTGGATGCCAATCCAGATGACCATGGTGAGATTGATTGGTGCGAAAAGGATTTGCTAGTTGCCTGTGGTAAGTTCAGACTTGGGAGTACTGAAGAAGAAATCACAGATCATCGTATGTTTTCTCATCAGGTGCATTGCAGAAAAGGCAAGGGTGAAGGCTCTTTTCTGGTATATCCTAGGAAGGGTGAAACTTGGGCCATCTACCAGAACTGGGGTGTTGGATGGAGTTCTGAGCCGGAAAAGCATGTGCCCTACACAAGTCAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

215

Amino Acids

23.9

Weight (kDa)

5.66

Isoelectric Point (pI)

27.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 64 - 210 3.2e-46 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 170
AciI CCGC 1 cut(s) 20
AclI AACGTT 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 486
AfaI GTAC 2 cut(s) 256, 463
AfiI CCNNNNNNNGG 4 cut(s) 19, 170, 557, 586
AgsI TTSAA 1 cut(s) 142
AluBI AGCT 4 cut(s) 63, 146, 266, 361
AluI AGCT 4 cut(s) 63, 146, 266, 361
Ama87I CYCGRG 1 cut(s) 349
AoxI GGCC 1 cut(s) 570
AspA2I CCTAGG 1 cut(s) 551
AspS9I GGNCC 2 cut(s) 35, 570
AsuC2I CCSGG 2 cut(s) 350, 351
AsuHPI GGTGA 4 cut(s) 305, 413, 539, 572
AvaI CYCGRG 1 cut(s) 349
AvaII GGWCC 1 cut(s) 35
AvrII CCTAGG 1 cut(s) 551
BaeGI GKGCMC 1 cut(s) 627
BanII GRGCYC 1 cut(s) 283
BccI CCATC 5 cut(s) 26, 284, 296, 581, 591
BcnI CCSGG 2 cut(s) 350, 351
BfaI CTAG 4 cut(s) 71, 87, 431, 552
BisI GCNGC 1 cut(s) 21
BlnI CCTAGG 1 cut(s) 551
BlsI GCNGC 1 cut(s) 22
BmcAI AGTACT 1 cut(s) 463
Bme1390I CCNGG 2 cut(s) 350, 351
Bme18I GGWCC 1 cut(s) 35
BmeT110I CYCGRG 1 cut(s) 349
BmgT120I GGNCC 2 cut(s) 35, 570
BmiI GGNNCC 2 cut(s) 111, 204
BmrFI CCNGG 2 cut(s) 350, 351
BmrI ACTGGG 2 cut(s) 114, 595
BmsI GCATC 2 cut(s) 188, 370
BmuI ACTGGG 2 cut(s) 114, 595
BpuMI CCSGG 2 cut(s) 350, 351
BsaBI GATNNNNATC 1 cut(s) 384
BsaJI CCNNGG 4 cut(s) 348, 349, 397, 551
Bsc4I CCNNNNNNNGG 4 cut(s) 19, 170, 557, 586
Bse1I ACTGG 2 cut(s) 109, 590
Bse3DI GCAATG 1 cut(s) 508
Bse8I GATNNNNATC 1 cut(s) 384
BseDI CCNNGG 4 cut(s) 348, 349, 397, 551
BseGI GGATG 4 cut(s) 37, 179, 385, 602
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 4 cut(s) 19, 170, 557, 586
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 1 cut(s) 597
BseNI ACTGG 2 cut(s) 109, 590
BseSI GKGCMC 1 cut(s) 627
BshFI GGCC 1 cut(s) 572
BsiHKCI CYCGRG 1 cut(s) 349
BsiSI CCGG 2 cut(s) 350, 611
BslI CCNNNNNNNGG 4 cut(s) 19, 170, 557, 586
BsnI GGCC 1 cut(s) 572
BsoBI CYCGRG 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 283, 627
Bsp143I GATC 1 cut(s) 481
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 1 cut(s) 20
BspANI GGCC 1 cut(s) 572
BspCNI CTCAG 1 cut(s) 598
BspLI GGNNCC 2 cut(s) 111, 204
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 2 cut(s) 109, 590
BssECI CCNNGG 4 cut(s) 348, 349, 397, 551
BssMI GATC 1 cut(s) 481
BssT1I CCWWGG 2 cut(s) 397, 551
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 606
BstDSI CCRYGG 1 cut(s) 397
BstEII GGTNACC 1 cut(s) 8
BstF5I GGATG 4 cut(s) 37, 179, 385, 602
BstKTI GATC 1 cut(s) 484
BstMBI GATC 1 cut(s) 481
BstMWI GCNNNNNNNGC 2 cut(s) 519, 616
BstNSI RCATGY 2 cut(s) 310, 623
BstPI GGTNACC 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 348, 349
BstSLI GKGCMC 1 cut(s) 627
BsuRI GGCC 1 cut(s) 572
BtgI CCRYGG 1 cut(s) 397
BtsCI GGATG 4 cut(s) 37, 179, 385, 602
Cac8I GCNNGC 1 cut(s) 308
Cfr13I GGNCC 2 cut(s) 35, 570
Cfr9I CCCGGG 1 cut(s) 349
Csp6I GTAC 2 cut(s) 255, 462
CviAII CATG 4 cut(s) 276, 307, 398, 620
CviJI RGCY 9 cut(s) 23, 63, 146, 266, 281, 361, 534, 572, 610
CviKI_1 RGCY 9 cut(s) 23, 63, 146, 266, 281, 361, 534, 572, 610
CviQI GTAC 2 cut(s) 255, 462
DdeI CTNAG 1 cut(s) 606
DpnI GATC 1 cut(s) 483
DpnII GATC 1 cut(s) 481
DraI TTTAAA 1 cut(s) 262
Eco130I CCWWGG 2 cut(s) 397, 551
Eco24I GRGCYC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 35
Eco57I CTGAAG 1 cut(s) 486
Eco88I CYCGRG 1 cut(s) 349
Eco91I GGTNACC 1 cut(s) 8
EcoO65I GGTNACC 1 cut(s) 8
EcoT14I CCWWGG 2 cut(s) 397, 551
EcoT38I GRGCYC 1 cut(s) 283
ErhI CCWWGG 2 cut(s) 397, 551
FaeI CATG 4 cut(s) 279, 310, 401, 623
FaiI YATR 9 cut(s) 222, 277, 308, 321, 399, 491, 547, 621, 643
FatI CATG 4 cut(s) 275, 306, 397, 619
Fnu4HI GCNGC 1 cut(s) 21
FokI GGATG 4 cut(s) 44, 166, 392, 609
FriOI GRGCYC 1 cut(s) 283
Fsp4HI GCNGC 1 cut(s) 21
FspBI CTAG 4 cut(s) 71, 87, 431, 552
GluI GCNGC 1 cut(s) 21
HaeIII GGCC 1 cut(s) 572
HapII CCGG 2 cut(s) 350, 611
Hin1II CATG 4 cut(s) 279, 310, 401, 623
HindIII AAGCTT 1 cut(s) 61
HpaII CCGG 2 cut(s) 350, 611
HphI GGTGA 4 cut(s) 305, 413, 539, 572
Hpy188I TCNGA 2 cut(s) 452, 607
Hpy188III TCNNGA 1 cut(s) 389
HpyAV CCTTC 2 cut(s) 524, 550
HpyCH4IV ACGT 1 cut(s) 245
HpyCH4V TGCA 3 cut(s) 179, 508, 513
HpyF10VI GCNNNNNNNGC 2 cut(s) 519, 616
HpyF3I CTNAG 1 cut(s) 606
HpySE526I ACGT 1 cut(s) 245
Hsp92II CATG 4 cut(s) 279, 310, 401, 623
Kzo9I GATC 1 cut(s) 481
LweI GCATC 2 cut(s) 188, 370
MaeI CTAG 4 cut(s) 71, 87, 431, 552
MaeII ACGT 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 8
MalI GATC 1 cut(s) 483
MboI GATC 1 cut(s) 481
MboII GAAGA 3 cut(s) 86, 479, 482
MhlI GDGCHC 2 cut(s) 283, 627
MluCI AATT 2 cut(s) 56, 215
MmeI TCCRAC 1 cut(s) 574
MnlI CCTC 2 cut(s) 193, 222
MseI TTAA 6 cut(s) 59, 153, 234, 261, 341, 357
MspI CCGG 2 cut(s) 350, 611
MspR9I CCNGG 2 cut(s) 350, 351
MwoI GCNNNNNNNGC 2 cut(s) 519, 616
NciI CCSGG 2 cut(s) 350, 351
NcoI CCATGG 1 cut(s) 397
NdeII GATC 1 cut(s) 481
NlaIII CATG 4 cut(s) 279, 310, 401, 623
NlaIV GGNNCC 2 cut(s) 111, 204
NmuCI GTSAC 1 cut(s) 8
NspI RCATGY 2 cut(s) 310, 623
PaeI GCATGC 1 cut(s) 310
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 1 cut(s) 22
Psp1406I AACGTT 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 8
PspN4I GGNNCC 2 cut(s) 111, 204
PspPI GGNCC 2 cut(s) 35, 570
RsaI GTAC 2 cut(s) 256, 463
RsaNI GTAC 2 cut(s) 255, 462
SaqAI TTAA 6 cut(s) 59, 153, 234, 261, 341, 357
SatI GCNGC 1 cut(s) 21
Sau3AI GATC 1 cut(s) 481
Sau96I GGNCC 2 cut(s) 35, 570
ScaI AGTACT 1 cut(s) 463
ScrFI CCNGG 2 cut(s) 350, 351
SduI GDGCHC 2 cut(s) 283, 627
SfaNI GCATC 2 cut(s) 188, 370
SinI GGWCC 1 cut(s) 35
SmaI CCCGGG 1 cut(s) 351
SphI GCATGC 1 cut(s) 310
Sse9I AATT 2 cut(s) 56, 215
SsiI CCGC 1 cut(s) 20
SspMI CTAG 4 cut(s) 71, 87, 431, 552
StyD4I CCNGG 2 cut(s) 348, 349
StyI CCWWGG 2 cut(s) 397, 551
TaiI ACGT 1 cut(s) 248
TasI AATT 2 cut(s) 56, 215
TatI WGTACW 1 cut(s) 461
TauI GCSGC 1 cut(s) 23
Tru1I TTAA 6 cut(s) 59, 153, 234, 261, 341, 357
Tru9I TTAA 6 cut(s) 59, 153, 234, 261, 341, 357
TseFI GTSAC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 2 cut(s) 144, 209
TspMI CCCGGG 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 35
XceI RCATGY 2 cut(s) 310, 623
XmaI CCCGGG 1 cut(s) 349
XmaJI CCTAGG 1 cut(s) 551
XspI CTAG 4 cut(s) 71, 87, 431, 552
ZrmI AGTACT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.