Domain of unknown function (DUF3444)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 407 |
| AccB1I | GGYRCC | 1 cut(s) | 407 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1700 |
| AciI | CCGC | 2 cut(s) | 311, 788 |
| AclWI | GGATC | 5 cut(s) | 49, 196, 1685, 1775, 2177 |
| AcsI | RAATTY | 2 cut(s) | 514, 907 |
| AcuI | CTGAAG | 5 cut(s) | 200, 822, 917, 1026, 1881 |
| AfaI | GTAC | 5 cut(s) | 409, 503, 537, 596, 2224 |
| AfeI | AGCGCT | 1 cut(s) | 397 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 64, 704, 1700, 1952, 1975, 1981 |
| AgeI | ACCGGT | 1 cut(s) | 544 |
| AgsI | TTSAA | 5 cut(s) | 714, 759, 875, 912, 1038 |
| AhlI | ACTAGT | 1 cut(s) | 506 |
| AjnI | CCWGG | 5 cut(s) | 1025, 1744, 1766, 1961, 1974 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 691, 723 |
| AleI | CACNNNNGTG | 1 cut(s) | 509 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1602, 1634 |
| AluBI | AGCT | 8 cut(s) | 121, 328, 572, 695, 722, 1929, 2129, 2270 |
| AluI | AGCT | 8 cut(s) | 121, 328, 572, 695, 722, 1929, 2129, 2270 |
| Alw21I | GWGCWC | 2 cut(s) | 1635, 2272 |
| AlwI | GGATC | 5 cut(s) | 49, 196, 1685, 1775, 2177 |
| Aor51HI | AGCGCT | 1 cut(s) | 397 |
| AoxI | GGCC | 2 cut(s) | 1230, 1965 |
| ApaI | GGGCCC | 1 cut(s) | 1969 |
| ApoI | RAATTY | 2 cut(s) | 514, 907 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 609, 641 |
| AsiGI | ACCGGT | 1 cut(s) | 544 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2346 |
| Asp718I | GGTACC | 1 cut(s) | 407 |
| AspA2I | CCTAGG | 2 cut(s) | 640, 1946 |
| AspLEI | GCGC | 4 cut(s) | 398, 1284, 1855, 2075 |
| AspS9I | GGNCC | 6 cut(s) | 449, 668, 890, 1231, 1965, 1966 |
| AsuHPI | GGTGA | 7 cut(s) | 541, 974, 1756, 1768, 1808, 2221, 2302 |
| AsuII | TTCGAA | 1 cut(s) | 1139 |
| AvaII | GGWCC | 3 cut(s) | 449, 668, 890 |
| AvrII | CCTAGG | 2 cut(s) | 640, 1946 |
| BaeGI | GKGCMC | 1 cut(s) | 1969 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 493, 526 |
| BanI | GGYRCC | 1 cut(s) | 407 |
| BanII | GRGCYC | 3 cut(s) | 1779, 1969, 2272 |
| BauI | CACGAG | 1 cut(s) | 580 |
| Bbv12I | GWGCWC | 2 cut(s) | 1635, 2272 |
| BbvCI | CCTCAGC | 1 cut(s) | 117 |
| BccI | CCATC | 4 cut(s) | 82, 760, 1196, 1694 |
| BciT130I | CCWGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| BciVI | GTATCC | 1 cut(s) | 740 |
| BclI | TGATCA | 1 cut(s) | 1789 |
| BcuI | ACTAGT | 1 cut(s) | 506 |
| BfaI | CTAG | 5 cut(s) | 507, 641, 1173, 1947, 2359 |
| BfoI | RGCGCY | 2 cut(s) | 399, 2076 |
| BfuI | GTATCC | 1 cut(s) | 740 |
| BglII | AGATCT | 1 cut(s) | 1031 |
| BlnI | CCTAGG | 2 cut(s) | 640, 1946 |
| BlpI | GCTNAGC | 1 cut(s) | 2130 |
| BmcAI | AGTACT | 1 cut(s) | 2224 |
| Bme1390I | CCNGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| Bme18I | GGWCC | 3 cut(s) | 449, 668, 890 |
| BmgT120I | GGNCC | 6 cut(s) | 449, 668, 890, 1231, 1965, 1966 |
| BmiI | GGNNCC | 8 cut(s) | 409, 451, 669, 814, 1605, 1776, 1967, 2330 |
| BmrFI | CCNGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| BmrI | ACTGGG | 1 cut(s) | 315 |
| BmsI | GCATC | 3 cut(s) | 626, 643, 1202 |
| BmuI | ACTGGG | 1 cut(s) | 315 |
| BpmI | CTGGAG | 2 cut(s) | 1793, 2097 |
| Bpu10I | CCTNAGC | 1 cut(s) | 117 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 2130 |
| Bpu14I | TTCGAA | 1 cut(s) | 1139 |
| BpuEI | CTTGAG | 1 cut(s) | 2131 |
| BsaBI | GATNNNNATC | 1 cut(s) | 751 |
| BsaWI | WCCGGW | 1 cut(s) | 544 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 64, 704, 1700, 1952, 1975, 1981 |
| Bse118I | RCCGGY | 2 cut(s) | 526, 544 |
| Bse1I | ACTGG | 9 cut(s) | 206, 310, 409, 462, 689, 748, 982, 1826, 2202 |
| Bse3DI | GCAATG | 2 cut(s) | 39, 1351 |
| Bse8I | GATNNNNATC | 1 cut(s) | 751 |
| BseBI | CCWGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| BseGI | GGATG | 4 cut(s) | 283, 1207, 1252, 1580 |
| BseJI | GATNNNNATC | 1 cut(s) | 751 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 64, 704, 1700, 1952, 1975, 1981 |
| BseMI | GCAATG | 2 cut(s) | 39, 1351 |
| BseMII | CTCAG | 7 cut(s) | 131, 506, 873, 1605, 1648, 1992, 2121 |
| BseNI | ACTGG | 9 cut(s) | 206, 310, 409, 462, 689, 748, 982, 1826, 2202 |
| BseRI | GAGGAG | 1 cut(s) | 951 |
| BseSI | GKGCMC | 1 cut(s) | 1969 |
| Bsh1285I | CGRYCG | 1 cut(s) | 2215 |
| BshFI | GGCC | 2 cut(s) | 1232, 1967 |
| BshNI | GGYRCC | 1 cut(s) | 407 |
| BshTI | ACCGGT | 1 cut(s) | 544 |
| BsiEI | CGRYCG | 1 cut(s) | 2215 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1635, 2272 |
| BsiSI | CCGG | 2 cut(s) | 527, 545 |
| BslFI | GGGAC | 1 cut(s) | 754 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 64, 704, 1700, 1952, 1975, 1981 |
| BsmFI | GGGAC | 1 cut(s) | 754 |
| BsmI | GAATGC | 3 cut(s) | 210, 949, 2014 |
| BsnI | GGCC | 2 cut(s) | 1232, 1967 |
| Bsp119I | TTCGAA | 1 cut(s) | 1139 |
| Bsp120I | GGGCCC | 1 cut(s) | 1965 |
| Bsp1286I | GDGCHC | 4 cut(s) | 1635, 1779, 1969, 2272 |
| Bsp1407I | TGTACA | 1 cut(s) | 535 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 2130 |
| Bsp19I | CCATGG | 4 cut(s) | 1332, 1359, 1467, 1494 |
| BspACI | CCGC | 2 cut(s) | 311, 788 |
| BspANI | GGCC | 2 cut(s) | 1232, 1967 |
| BspCNI | CTCAG | 7 cut(s) | 130, 505, 874, 1606, 1647, 1993, 2122 |
| BspHI | TCATGA | 1 cut(s) | 1891 |
| BspLI | GGNNCC | 8 cut(s) | 409, 451, 669, 814, 1605, 1776, 1967, 2330 |
| BspPI | GGATC | 5 cut(s) | 49, 196, 1685, 1775, 2177 |
| BspT104I | TTCGAA | 1 cut(s) | 1139 |
| BspT107I | GGYRCC | 1 cut(s) | 407 |
| BsrDI | GCAATG | 2 cut(s) | 39, 1351 |
| BsrFI | RCCGGY | 2 cut(s) | 526, 544 |
| BsrGI | TGTACA | 1 cut(s) | 535 |
| BsrI | ACTGG | 9 cut(s) | 206, 310, 409, 462, 689, 748, 982, 1826, 2202 |
| BssAI | RCCGGY | 2 cut(s) | 526, 544 |
| BssSI | CACGAG | 1 cut(s) | 580 |
| BssT1I | CCWWGG | 7 cut(s) | 361, 640, 1332, 1359, 1467, 1494, 1946 |
| Bst2BI | CACGAG | 1 cut(s) | 580 |
| Bst2UI | CCWGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| Bst6I | CTCTTC | 1 cut(s) | 1175 |
| BstAUI | TGTACA | 1 cut(s) | 535 |
| BstBI | TTCGAA | 1 cut(s) | 1139 |
| BstC8I | GCNNGC | 5 cut(s) | 574, 1706, 2127, 2199, 2272 |
| BstDEI | CTNAG | 9 cut(s) | 117, 492, 819, 882, 1614, 1634, 2001, 2130, 2280 |
| BstDSI | CCRYGG | 9 cut(s) | 1332, 1359, 1386, 1413, 1440, 1467, 1494, 1521, 1548 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1973 |
| BstF5I | GGATG | 4 cut(s) | 283, 1207, 1252, 1580 |
| BstH2I | RGCGCY | 2 cut(s) | 399, 2076 |
| BstHHI | GCGC | 4 cut(s) | 398, 1284, 1855, 2075 |
| BstMCI | CGRYCG | 1 cut(s) | 2215 |
| BstNI | CCWGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| BstNSI | RCATGY | 3 cut(s) | 1708, 1725, 1836 |
| BstSCI | CCNGG | 5 cut(s) | 1025, 1744, 1766, 1961, 1974 |
| BstSLI | GKGCMC | 1 cut(s) | 1969 |
| BstX2I | RGATCY | 2 cut(s) | 54, 1031 |
| BstYI | RGATCY | 2 cut(s) | 54, 1031 |
| BsuI | GTATCC | 1 cut(s) | 740 |
| BsuRI | GGCC | 2 cut(s) | 1232, 1967 |
| BtgI | CCRYGG | 9 cut(s) | 1332, 1359, 1386, 1413, 1440, 1467, 1494, 1521, 1548 |
| BtsCI | GGATG | 4 cut(s) | 283, 1207, 1252, 1580 |
| BtsI | GCAGTG | 1 cut(s) | 1164 |
| Cac8I | GCNNGC | 5 cut(s) | 574, 1706, 2127, 2199, 2272 |
| CciI | TCATGA | 1 cut(s) | 1891 |
| CfoI | GCGC | 4 cut(s) | 398, 1284, 1855, 2075 |
| Cfr10I | RCCGGY | 2 cut(s) | 526, 544 |
| Cfr13I | GGNCC | 6 cut(s) | 449, 668, 890, 1231, 1965, 1966 |
| CseI | GACGC | 1 cut(s) | 1722 |
| Csp6I | GTAC | 5 cut(s) | 408, 502, 536, 595, 2223 |
| CspAI | ACCGGT | 1 cut(s) | 544 |
| CviQI | GTAC | 5 cut(s) | 408, 502, 536, 595, 2223 |
| DdeI | CTNAG | 9 cut(s) | 117, 492, 819, 882, 1614, 1634, 2001, 2130, 2280 |
| DraI | TTTAAA | 2 cut(s) | 73, 1663 |
| Eam1104I | CTCTTC | 1 cut(s) | 1175 |
| EarI | CTCTTC | 1 cut(s) | 1175 |
| EciI | GGCGGA | 1 cut(s) | 326 |
| Ecl136II | GAGCTC | 1 cut(s) | 2270 |
| Eco130I | CCWWGG | 7 cut(s) | 361, 640, 1332, 1359, 1467, 1494, 1946 |
| Eco24I | GRGCYC | 3 cut(s) | 1779, 1969, 2272 |
| Eco32I | GATATC | 3 cut(s) | 838, 1504, 1531 |
| Eco47I | GGWCC | 3 cut(s) | 449, 668, 890 |
| Eco47III | AGCGCT | 1 cut(s) | 397 |
| Eco53kI | GAGCTC | 1 cut(s) | 2270 |
| Eco57I | CTGAAG | 5 cut(s) | 200, 822, 917, 1026, 1881 |
| EcoICRI | GAGCTC | 1 cut(s) | 2270 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1973 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1966 |
| EcoRI | GAATTC | 1 cut(s) | 907 |
| EcoRII | CCWGG | 5 cut(s) | 1025, 1744, 1766, 1961, 1974 |
| EcoRV | GATATC | 3 cut(s) | 838, 1504, 1531 |
| EcoT14I | CCWWGG | 7 cut(s) | 361, 640, 1332, 1359, 1467, 1494, 1946 |
| EcoT22I | ATGCAT | 3 cut(s) | 1050, 1195, 1723 |
| EcoT38I | GRGCYC | 3 cut(s) | 1779, 1969, 2272 |
| ErhI | CCWWGG | 7 cut(s) | 361, 640, 1332, 1359, 1467, 1494, 1946 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1721, 1753 |
| FaqI | GGGAC | 1 cut(s) | 754 |
| FbaI | TGATCA | 1 cut(s) | 1789 |
| FokI | GGATG | 4 cut(s) | 270, 1214, 1259, 1567 |
| FriOI | GRGCYC | 3 cut(s) | 1779, 1969, 2272 |
| FspBI | CTAG | 5 cut(s) | 507, 641, 1173, 1947, 2359 |
| GlaI | GCGC | 4 cut(s) | 397, 1283, 1854, 2074 |
| GsuI | CTGGAG | 2 cut(s) | 1793, 2097 |
| HaeII | RGCGCY | 2 cut(s) | 399, 2076 |
| HaeIII | GGCC | 2 cut(s) | 1232, 1967 |
| HapII | CCGG | 2 cut(s) | 527, 545 |
| HgaI | GACGC | 1 cut(s) | 1722 |
| HhaI | GCGC | 4 cut(s) | 398, 1284, 1855, 2075 |
| Hin6I | GCGC | 4 cut(s) | 396, 1282, 1853, 2073 |
| HinP1I | GCGC | 4 cut(s) | 396, 1282, 1853, 2073 |
| HindIII | AAGCTT | 1 cut(s) | 720 |
| HpaII | CCGG | 2 cut(s) | 527, 545 |
| HphI | GGTGA | 7 cut(s) | 541, 974, 1756, 1768, 1808, 2221, 2302 |
| Hpy166II | GTNNAC | 4 cut(s) | 160, 303, 504, 893 |
| Hpy8I | GTNNAC | 4 cut(s) | 160, 303, 504, 893 |
| HpyAV | CCTTC | 6 cut(s) | 172, 797, 1011, 1146, 1945, 2268 |
| HpyCH4IV | ACGT | 1 cut(s) | 779 |
| HpyF3I | CTNAG | 9 cut(s) | 117, 492, 819, 882, 1614, 1634, 2001, 2130, 2280 |
| HpySE526I | ACGT | 1 cut(s) | 779 |
| HspAI | GCGC | 4 cut(s) | 396, 1282, 1853, 2073 |
| KpnI | GGTACC | 1 cut(s) | 411 |
| Ksp22I | TGATCA | 1 cut(s) | 1789 |
| LmnI | GCTCC | 4 cut(s) | 77, 700, 812, 1774 |
| LweI | GCATC | 3 cut(s) | 626, 643, 1202 |
| MaeI | CTAG | 5 cut(s) | 507, 641, 1173, 1947, 2359 |
| MaeII | ACGT | 1 cut(s) | 779 |
| MaeIII | GTNAC | 4 cut(s) | 495, 1311, 1647, 2227 |
| MboII | GAAGA | 8 cut(s) | 206, 702, 747, 1026, 1192, 1874, 2229, 2305 |
| MflI | RGATCY | 2 cut(s) | 54, 1031 |
| MhlI | GDGCHC | 4 cut(s) | 1635, 1779, 1969, 2272 |
| MlyI | GAGTC | 4 cut(s) | 102, 612, 893, 1141 |
| MmeI | TCCRAC | 1 cut(s) | 832 |
| Mph1103I | ATGCAT | 3 cut(s) | 1050, 1195, 1723 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2346 |
| MseI | TTAA | 7 cut(s) | 72, 140, 324, 401, 438, 1188, 1662 |
| MslI | CAYNNNNRTG | 2 cut(s) | 462, 509 |
| MspA1I | CMGCKG | 1 cut(s) | 2129 |
| MspI | CCGG | 2 cut(s) | 527, 545 |
| MspR9I | CCNGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| Mva1269I | GAATGC | 3 cut(s) | 210, 949, 2014 |
| MvaI | CCWGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| NcoI | CCATGG | 4 cut(s) | 1332, 1359, 1467, 1494 |
| NlaIV | GGNNCC | 8 cut(s) | 409, 451, 669, 814, 1605, 1776, 1967, 2330 |
| NmuCI | GTSAC | 2 cut(s) | 495, 2227 |
| NsiI | ATGCAT | 3 cut(s) | 1050, 1195, 1723 |
| NspI | RCATGY | 3 cut(s) | 1708, 1725, 1836 |
| NspV | TTCGAA | 1 cut(s) | 1139 |
| OliI | CACNNNNGTG | 1 cut(s) | 509 |
| PaeI | GCATGC | 1 cut(s) | 1708 |
| PagI | TCATGA | 1 cut(s) | 1891 |
| PctI | GAATGC | 3 cut(s) | 210, 949, 2014 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2346 |
| PfeI | GAWTC | 6 cut(s) | 220, 645, 674, 685, 956, 2300 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1700 |
| PinAI | ACCGGT | 1 cut(s) | 544 |
| Ple19I | CGATCG | 1 cut(s) | 2215 |
| PleI | GAGTC | 4 cut(s) | 102, 612, 892, 1140 |
| PpsI | GAGTC | 4 cut(s) | 102, 612, 892, 1140 |
| Psp124BI | GAGCTC | 1 cut(s) | 2272 |
| Psp6I | CCWGG | 5 cut(s) | 1025, 1744, 1766, 1961, 1974 |
| PspGI | CCWGG | 5 cut(s) | 1025, 1744, 1766, 1961, 1974 |
| PspN4I | GGNNCC | 8 cut(s) | 409, 451, 669, 814, 1605, 1776, 1967, 2330 |
| PspOMI | GGGCCC | 1 cut(s) | 1965 |
| PspPI | GGNCC | 6 cut(s) | 449, 668, 890, 1231, 1965, 1966 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 141, 173 |
| PsuI | RGATCY | 2 cut(s) | 54, 1031 |
| PvuI | CGATCG | 1 cut(s) | 2215 |
| PvuII | CAGCTG | 1 cut(s) | 2129 |
| RsaI | GTAC | 5 cut(s) | 409, 503, 537, 596, 2224 |
| RsaNI | GTAC | 5 cut(s) | 408, 502, 536, 595, 2223 |
| RseI | CAYNNNNRTG | 2 cut(s) | 462, 509 |
| SacI | GAGCTC | 1 cut(s) | 2272 |
| SaqAI | TTAA | 7 cut(s) | 72, 140, 324, 401, 438, 1188, 1662 |
| Sau96I | GGNCC | 6 cut(s) | 449, 668, 890, 1231, 1965, 1966 |
| ScaI | AGTACT | 1 cut(s) | 2224 |
| SchI | GAGTC | 4 cut(s) | 102, 612, 893, 1141 |
| ScrFI | CCNGG | 5 cut(s) | 1027, 1746, 1768, 1963, 1976 |
| SduI | GDGCHC | 4 cut(s) | 1635, 1779, 1969, 2272 |
| SfaNI | GCATC | 3 cut(s) | 626, 643, 1202 |
| SfuI | TTCGAA | 1 cut(s) | 1139 |
| SinI | GGWCC | 3 cut(s) | 449, 668, 890 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 462, 509 |
| SmlI | CTYRAG | 1 cut(s) | 2146 |
| SmoI | CTYRAG | 1 cut(s) | 2146 |
| SpeI | ACTAGT | 1 cut(s) | 506 |
| SphI | GCATGC | 1 cut(s) | 1708 |
| SsiI | CCGC | 2 cut(s) | 311, 788 |
| SspI | AATATT | 1 cut(s) | 487 |
| SspMI | CTAG | 5 cut(s) | 507, 641, 1173, 1947, 2359 |
| SstI | GAGCTC | 1 cut(s) | 2272 |
| StyD4I | CCNGG | 5 cut(s) | 1025, 1744, 1766, 1961, 1974 |
| StyI | CCWWGG | 7 cut(s) | 361, 640, 1332, 1359, 1467, 1494, 1946 |
| TaiI | ACGT | 1 cut(s) | 782 |
| TaqI | TCGA | 5 cut(s) | 616, 1139, 1599, 2211, 2215 |
| TatI | WGTACW | 2 cut(s) | 535, 2222 |
| TauI | GCSGC | 1 cut(s) | 791 |
| TfiI | GAWTC | 6 cut(s) | 220, 645, 674, 685, 956, 2300 |
| Tru1I | TTAA | 7 cut(s) | 72, 140, 324, 401, 438, 1188, 1662 |
| Tru9I | TTAA | 7 cut(s) | 72, 140, 324, 401, 438, 1188, 1662 |
| TseFI | GTSAC | 2 cut(s) | 495, 2227 |
| Tsp45I | GTSAC | 2 cut(s) | 495, 2227 |
| TspDTI | ATGAA | 8 cut(s) | 252, 272, 1080, 1125, 1317, 1338, 2242, 2355 |
| TspGWI | ACGGA | 5 cut(s) | 1375, 1402, 1429, 1510, 1537 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1700 |
| VpaK11BI | GGWCC | 3 cut(s) | 449, 668, 890 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1973 |
| XapI | RAATTY | 2 cut(s) | 514, 907 |
| XceI | RCATGY | 3 cut(s) | 1708, 1725, 1836 |
| XmaJI | CCTAGG | 2 cut(s) | 640, 1946 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2346 |
| XspI | CTAG | 5 cut(s) | 507, 641, 1173, 1947, 2359 |
| ZrmI | AGTACT | 1 cut(s) | 2224 |
| Zsp2I | ATGCAT | 3 cut(s) | 1050, 1195, 1723 |