pycom04g18920

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000587
Physical Location & Seq
Reverse (-)
20750561 .. 20752876
2316 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18920.1

Sequence Viewer

Length: 2316 bp
ATGAAGGTCATCACCATAGGAGATCGTTCGAGGATCAAACAGAATCTTTCTTACAATGAAAATCTAAAAGATGATGATGATTTTGTGAGTCTGCCCAAAAGGTCGACGGATAGTCAATTTTCCAGTGATACTGAAATGGAGAGGAAGAATGCAGCTGCGGATGTTGGGGTATCAAAAAATAATAGTCAACCAGGTTGTGCTACTACTGCTGCAGTGGGTGGACATAAGAAAGAGGCAAAACAAAAAGTGAATGCCCCTCTAGAAGAATGTTTGCCCAGCAAAAGAAGCAAAACTGCTGAATTCGAGCAAAAGGTGGAGGAAGCAGCTATGCCAGATAAAGATGACGATAAGTCCAAAGCTGATAATGGTTCTGGACCAAATTCAAATGTCACATCCACATCCATACCAGCAGGCGTTCTGGTTCCTGATCCTGAATTCAATAAGTTCATGCTTGATGTGGATACGTTAGAGAATGTGTTTAGTGCTAATCAAACATGGGCTCTTTACGATCCACTAGATGGACTGCCCAGATTCTATGCTCGTATCAAGAAAGTATTCTCCCCTGGATTTAAGCTGAGATTCACCTGGTTGGAGCCCAGTCCAGATGACCAACGTGAGGTTGCTTGGTGTAACAAGGAGATGCCTGTTGCTTGTGGCAAGTACACGGTTGGTGCCACTGCAGAAGTTACAGATCATCTTATGTTTTCTCATCAGATGCACTGCATAAAAGGCAGTGGCAGAAGCTCTTTTTTCGTATATCCTAAGAAGGGAGAAACCTGGGCTCTCTACCAGAATTGGGATATTGGGTGGAGTTCTGAGCCTGAAAAGCATTTACCGTACAAGTTTGATTTTGTGGAAGTATTGTCGGATTTTGTCGAAAATTATGGTGTTGGAGTTGCTTATCTGGGCAAAGTAAAAGGTTTTGTCAGTCTATTTCAGCGAAGGGAGCAGCATGGGGTTGTCATGTTTCAAGTACTACCGAATGAGCTATATCGATTTTCTCATCGAATTCCCTCTTTCAAGATGACAGGTACTGAGAGAGAGGGTGTTCCAGAAGGATCCTTTGAGATCGATCCTGCTGCTTTGCCCACCAATTCTGACGACCTCATTGCTTCGAAGACGGACAACAGAACCATGAATACTGAAGCTAATGGTTCGTCATGTGACATAAGCGAAAGCAAAACCAAACCTGTAATGAGTTCTGGGAAAGCATGCCCCGCTGAGAAACAGGAGAAGAACTATTCAGAAAGAGAGACTTCAACACGTAGATCTCCACGAATGTCAAATGGCAAGTTTGCGAATTCTGTTAGGCTAGAAGCAACTCAAGGTATTACTGAAGACAATGGCACAAACCAGGGTAACCAAACTCAACCTAGGGGAAGTTCTACTCCGCGGGAGACTGATGAGAGTAACAACACACCAAAGAAGCATCAGAAAAATGATTCCGATGGTAAGTCTTTCAGCCTTAGAAGATCACCAAGGGATGTAAGCAAGAACATTCCGAGACCAAATGTAACTGTGAAGTGCCCAGATTCCGCCAACGATACAAGCCATGCCAGCTTCACTCCGATGAAAGTCAACTCTACTTCTAGTCAGGCACATTCAAGTGTGAGGGATCATCCTTCTGTCAGTTCCTTGAAAATCCCCGTCGCCCCTTCATCTTCATCTCCTCTCCATATTTTATCCCGATCTCAGTTCTATGACTTTGATGGGCAAAAGTCCGAGGACAAATTTCGGCTTGATCAAATCTGGGCACTGTATAGTGACAAGAATGGGATGCCTAGGACTTACGCACAAGTTAAGAAGATTGTGTTGAAGCCAAAATTTCAACTCCATGTAGCGTTGCTCGAACCTTGCTTACCAGAAGACACGACTGAACCGGTTTGTTGTGGGACATTTAAAGTCAAGAGTGGTCAAACCGTGGTCTTCTCCCGCTCGTCATTCTCTCATTGTGTGAAAGCCAAACCTATTGGGAAGACGATGTTTGAAATATACCCCAGGAAAGGCGAAGTTTGGGCATTATACAAGAACTCAGGGAAGAGTGAGTGTGAAATGGTACAAGTCCTGGAAGATAACGAGGAAAGCACAAAGGTTGCGGTTCTGCCACACGTTAACGGATCCAAGTCGTTATTTAGGGCCCCCAGAATCCACAGATCGAAGAATGGCATCATAGACATACCAAGGGCTGAGTTAGGTAGATTCTCTCACCAAGTTCCGGCTTTTCTTCATACCGGGGAGATTGACATCCAGCTTGCAAGCTGCTGGGAGGTTGATCAGTTATCAATTCCTGGTACTATTACAATCGTTTTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

772

Amino Acids

85.86

Weight (kDa)

8.04

Isoelectric Point (pI)

42.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 140 - 346 2.3e-76 Domain of unknown function (DUF3444)
DUF3444 PF11926 562 - 746 2.4e-52 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 671
AccB7I CCANNNNNTGG 1 cut(s) 518
AccBSI CCGCTC 1 cut(s) 1935
AccI GTMKAC 1 cut(s) 104
AccII CGCG 1 cut(s) 1393
AciI CCGC 7 cut(s) 158, 1218, 1391, 1393, 1536, 1933, 2096
AclWI GGATC 9 cut(s) 41, 422, 503, 1053, 1066, 1067, 1623, 2112, 2125
AcsI RAATTY 7 cut(s) 299, 379, 434, 1008, 1300, 1730, 1823
AcuI CTGAAG 2 cut(s) 1164, 1356
AfaI GTAC 6 cut(s) 662, 839, 975, 1033, 2058, 2293
AfiI CCNNNNNNNGG 6 cut(s) 518, 616, 767, 796, 2003, 2215
AflIII ACRYGT 2 cut(s) 1262, 2107
AgeI ACCGGT 1 cut(s) 1879
AhdI GACNNNNNGTC 2 cut(s) 111, 349
AjnI CCWGG 8 cut(s) 190, 562, 584, 776, 1353, 1997, 2064, 2287
Alw26I GTCTC 3 cut(s) 1247, 1391, 1498
AlwI GGATC 9 cut(s) 41, 422, 503, 1053, 1066, 1067, 1623, 2112, 2125
AlwNI CAGNNNCTG 1 cut(s) 1034
AoxI GGCC 1 cut(s) 2136
ApaI GGGCCC 1 cut(s) 2140
ApeKI GCWGC 7 cut(s) 152, 155, 209, 323, 949, 1079, 2259
ApoI RAATTY 7 cut(s) 299, 379, 434, 1008, 1300, 1730, 1823
AsiGI ACCGGT 1 cut(s) 1879
Asp700I GAANNNNTTC 1 cut(s) 554
AspA2I CCTAGG 2 cut(s) 1373, 1781
AspS9I GGNCC 3 cut(s) 374, 2136, 2137
AsuC2I CCSGG 1 cut(s) 2233
AsuHPI GGTGA 4 cut(s) 4, 574, 1467, 2198
AsuII TTCGAA 1 cut(s) 1115
AvaII GGWCC 1 cut(s) 374
AvrII CCTAGG 2 cut(s) 1373, 1781
BaeGI GKGCMC 3 cut(s) 1529, 1756, 2140
BamHI GGATCC 2 cut(s) 1058, 2117
BanI GGYRCC 1 cut(s) 671
BanII GRGCYC 4 cut(s) 502, 597, 784, 2140
BbsI GAAGAC 5 cut(s) 1124, 1344, 1872, 1918, 1982
BbvI GCAGC 7 cut(s) 142, 164, 196, 335, 961, 1066, 2246
BccI CCATC 3 cut(s) 512, 1442, 1703
BcgI CGANNNNNNTGC 4 cut(s) 1061, 1091, 1095, 1125
BciT130I CCWGG 8 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2289
BciVI GTATCC 1 cut(s) 454
BclI TGATCA 2 cut(s) 1741, 2272
BcnI CCSGG 1 cut(s) 2233
BcoDI GTCTC 3 cut(s) 1247, 1391, 1498
BfaI CTAG 6 cut(s) 260, 515, 1313, 1374, 1590, 1782
BfmI CTRYAG 2 cut(s) 210, 678
BfuI GTATCC 1 cut(s) 454
BglII AGATCT 1 cut(s) 1268
BisI GCNGC 7 cut(s) 153, 156, 210, 324, 950, 1080, 2260
BlnI CCTAGG 2 cut(s) 1373, 1781
BlsI GCNGC 7 cut(s) 154, 157, 211, 325, 951, 1081, 2261
BmcAI AGTACT 1 cut(s) 975
Bme1390I CCNGG 9 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2233, 2289
Bme18I GGWCC 1 cut(s) 374
BmeRI GACNNNNNGTC 2 cut(s) 111, 349
BmgT120I GGNCC 3 cut(s) 374, 2136, 2137
BmiI GGNNCC 7 cut(s) 423, 594, 673, 1060, 2119, 2138, 2139
BmrFI CCNGG 9 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2233, 2289
BmrI ACTGGG 1 cut(s) 591
BmsI GCATC 5 cut(s) 630, 705, 1438, 1767, 2175
BmuI ACTGGG 1 cut(s) 591
BpiI GAAGAC 5 cut(s) 1124, 1344, 1872, 1918, 1982
Bpu14I TTCGAA 1 cut(s) 1115
BpuEI CTTGAG 1 cut(s) 1308
BpuMI CCSGG 1 cut(s) 2233
Bsa29I ATCGAT 2 cut(s) 994, 1071
BsaAI YACGTR 1 cut(s) 1265
BsaBI GATNNNNATC 1 cut(s) 2243
BsaI GGTCTC 1 cut(s) 1498
BsaWI WCCGGW 1 cut(s) 1879
Bsc4I CCNNNNNNNGG 6 cut(s) 518, 616, 767, 796, 2003, 2215
Bse118I RCCGGY 1 cut(s) 1879
Bse1I ACTGG 2 cut(s) 123, 597
Bse3DI GCAATG 1 cut(s) 1107
Bse8I GATNNNNATC 1 cut(s) 2243
BseBI CCWGG 8 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2289
BseCI ATCGAT 2 cut(s) 994, 1071
BseGI GGATG 7 cut(s) 166, 392, 398, 1489, 1618, 1782, 2244
BseJI GATNNNNATC 1 cut(s) 2243
BseLI CCNNNNNNNGG 6 cut(s) 518, 616, 767, 796, 2003, 2215
BseMI GCAATG 1 cut(s) 1107
BseMII CTCAG 7 cut(s) 566, 807, 1026, 1212, 1706, 2046, 2178
BseNI ACTGG 2 cut(s) 123, 597
BseRI GAGGAG 1 cut(s) 1659
BseSI GKGCMC 3 cut(s) 1529, 1756, 2140
BseXI GCAGC 7 cut(s) 142, 164, 196, 335, 961, 1066, 2246
BseYI CCCAGC 2 cut(s) 275, 2262
Bsh1236I CGCG 1 cut(s) 1393
BshFI GGCC 1 cut(s) 2138
BshNI GGYRCC 1 cut(s) 671
BshTI ACCGGT 1 cut(s) 1879
BshVI ATCGAT 2 cut(s) 994, 1071
BsiSI CCGG 3 cut(s) 1880, 2216, 2232
BslFI GGGAC 1 cut(s) 1906
BslI CCNNNNNNNGG 6 cut(s) 518, 616, 767, 796, 2003, 2215
BsmAI GTCTC 3 cut(s) 1247, 1391, 1498
BsmFI GGGAC 1 cut(s) 1906
BsmI GAATGC 2 cut(s) 154, 256
BsnI GGCC 1 cut(s) 2138
Bso31I GGTCTC 1 cut(s) 1498
Bsp119I TTCGAA 1 cut(s) 1115
Bsp120I GGGCCC 1 cut(s) 2136
Bsp1286I GDGCHC 6 cut(s) 502, 597, 784, 1529, 1756, 2140
BspACI CCGC 7 cut(s) 158, 1218, 1391, 1393, 1536, 1933, 2096
BspANI GGCC 1 cut(s) 2138
BspCNI CTCAG 7 cut(s) 567, 808, 1027, 1213, 1705, 2045, 2179
BspDI ATCGAT 2 cut(s) 994, 1071
BspFNI CGCG 1 cut(s) 1393
BspLI GGNNCC 7 cut(s) 423, 594, 673, 1060, 2119, 2138, 2139
BspMAI CTGCAG 2 cut(s) 214, 682
BspPI GGATC 9 cut(s) 41, 422, 503, 1053, 1066, 1067, 1623, 2112, 2125
BspT104I TTCGAA 1 cut(s) 1115
BspT107I GGYRCC 1 cut(s) 671
BspTNI GGTCTC 1 cut(s) 1498
BsrBI CCGCTC 1 cut(s) 1935
BsrDI GCAATG 1 cut(s) 1107
BsrFI RCCGGY 1 cut(s) 1879
BsrI ACTGG 2 cut(s) 123, 597
BssAI RCCGGY 1 cut(s) 1879
BssT1I CCWWGG 4 cut(s) 1373, 1478, 1781, 2180
Bst2UI CCWGG 8 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2289
Bst4CI ACNGT 5 cut(s) 667, 837, 1519, 1758, 1921
Bst6I CTCTTC 1 cut(s) 2033
BstBAI YACGTR 1 cut(s) 1265
BstBI TTCGAA 1 cut(s) 1115
BstC8I GCNNGC 5 cut(s) 412, 1213, 1558, 2253, 2257
BstDEI CTNAG 9 cut(s) 575, 762, 816, 1035, 1221, 1466, 1692, 2032, 2187
BstDSI CCRYGG 2 cut(s) 1391, 1920
BstEII GGTNACC 1 cut(s) 1358
BstF5I GGATG 7 cut(s) 166, 392, 398, 1489, 1618, 1782, 2244
BstFNI CGCG 1 cut(s) 1393
BstMAI GTCTC 3 cut(s) 1247, 1391, 1498
BstMWI GCNNNNNNNGC 7 cut(s) 206, 285, 729, 826, 946, 1217, 1557
BstNI CCWGG 8 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2289
BstNSI RCATGY 1 cut(s) 1215
BstPI GGTNACC 1 cut(s) 1358
BstSCI CCNGG 9 cut(s) 190, 562, 584, 776, 1353, 1997, 2064, 2231, 2287
BstSFI CTRYAG 2 cut(s) 210, 678
BstSLI GKGCMC 3 cut(s) 1529, 1756, 2140
BstUI CGCG 1 cut(s) 1393
BstV1I GCAGC 7 cut(s) 142, 164, 196, 335, 961, 1066, 2246
BstV2I GAAGAC 5 cut(s) 1124, 1344, 1872, 1918, 1982
BstX2I RGATCY 3 cut(s) 1058, 1268, 2117
BstYI RGATCY 3 cut(s) 1058, 1268, 2117
Bsu15I ATCGAT 2 cut(s) 994, 1071
BsuI GTATCC 1 cut(s) 454
BsuRI GGCC 1 cut(s) 2138
BsuTUI ATCGAT 2 cut(s) 994, 1071
BtgI CCRYGG 2 cut(s) 1391, 1920
BtsCI GGATG 7 cut(s) 166, 392, 398, 1489, 1618, 1782, 2244
BtsI GCAGTG 4 cut(s) 219, 675, 718, 739
BtsIMutI CAGTG 6 cut(s) 130, 219, 675, 718, 739, 1754
Cac8I GCNNGC 5 cut(s) 412, 1213, 1558, 2253, 2257
CaiI CAGNNNCTG 1 cut(s) 1034
Cfr10I RCCGGY 1 cut(s) 1879
Cfr13I GGNCC 3 cut(s) 374, 2136, 2137
Cfr42I CCGCGG 1 cut(s) 1394
ClaI ATCGAT 2 cut(s) 994, 1071
CsiI ACCWGGT 2 cut(s) 190, 584
Csp6I GTAC 6 cut(s) 661, 838, 974, 1032, 2057, 2292
CspAI ACCGGT 1 cut(s) 1879
CviAII CATG 9 cut(s) 448, 495, 953, 964, 1135, 1161, 1212, 1553, 1835
CviQI GTAC 6 cut(s) 661, 838, 974, 1032, 2057, 2292
DdeI CTNAG 9 cut(s) 575, 762, 816, 1035, 1221, 1466, 1692, 2032, 2187
DraI TTTAAA 1 cut(s) 1900
DriI GACNNNNNGTC 2 cut(s) 111, 349
Eam1104I CTCTTC 1 cut(s) 2033
Eam1105I GACNNNNNGTC 2 cut(s) 111, 349
EarI CTCTTC 1 cut(s) 2033
EciI GGCGGA 1 cut(s) 1525
Eco130I CCWWGG 4 cut(s) 1373, 1478, 1781, 2180
Eco24I GRGCYC 4 cut(s) 502, 597, 784, 2140
Eco31I GGTCTC 1 cut(s) 1498
Eco47I GGWCC 1 cut(s) 374
Eco57I CTGAAG 2 cut(s) 1164, 1356
Eco91I GGTNACC 1 cut(s) 1358
EcoO109I RGGNCCY 2 cut(s) 2136, 2137
EcoO65I GGTNACC 1 cut(s) 1358
EcoRI GAATTC 4 cut(s) 299, 434, 1008, 1300
EcoRII CCWGG 8 cut(s) 190, 562, 584, 776, 1353, 1997, 2064, 2287
EcoT14I CCWWGG 4 cut(s) 1373, 1478, 1781, 2180
EcoT38I GRGCYC 4 cut(s) 502, 597, 784, 2140
ErhI CCWWGG 4 cut(s) 1373, 1478, 1781, 2180
FaeI CATG 9 cut(s) 451, 498, 956, 967, 1138, 1164, 1215, 1556, 1838
FalI AAGNNNNNCTT 2 cut(s) 1240, 1272
FaqI GGGAC 1 cut(s) 1906
FatI CATG 9 cut(s) 447, 494, 952, 963, 1134, 1160, 1211, 1552, 1834
FauI CCCGC 3 cut(s) 1225, 1386, 1940
FbaI TGATCA 2 cut(s) 1741, 2272
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 7 cut(s) 153, 156, 210, 324, 950, 1080, 2260
FokI GGATG 7 cut(s) 173, 379, 385, 1496, 1605, 1789, 2231
FriOI GRGCYC 4 cut(s) 502, 597, 784, 2140
Fsp4HI GCNGC 7 cut(s) 153, 156, 210, 324, 950, 1080, 2260
FspBI CTAG 6 cut(s) 260, 515, 1313, 1374, 1590, 1782
GluI GCNGC 7 cut(s) 153, 156, 210, 324, 950, 1080, 2260
GsaI CCCAGC 2 cut(s) 279, 2266
HaeIII GGCC 1 cut(s) 2138
HapII CCGG 3 cut(s) 1880, 2216, 2232
Hin1II CATG 9 cut(s) 451, 498, 956, 967, 1138, 1164, 1215, 1556, 1838
HincII GTYRAC 4 cut(s) 105, 188, 1579, 2113
HindII GTYRAC 4 cut(s) 105, 188, 1579, 2113
HinfI GANTC 8 cut(s) 43, 88, 531, 579, 1442, 1532, 2145, 2199
HpaI GTTAAC 1 cut(s) 2113
HpaII CCGG 3 cut(s) 1880, 2216, 2232
HphI GGTGA 4 cut(s) 4, 574, 1467, 2198
Hpy166II GTNNAC 6 cut(s) 105, 188, 221, 663, 1579, 2113
Hpy8I GTNNAC 6 cut(s) 105, 188, 221, 663, 1579, 2113
Hpy99I CGWCG 2 cut(s) 109, 1652
HpyAV CCTTC 5 cut(s) 760, 936, 1049, 1632, 1665
HpyCH4III ACNGT 5 cut(s) 667, 837, 1519, 1758, 1921
HpyCH4IV ACGT 4 cut(s) 464, 613, 1264, 2109
HpyCH4V TGCA 6 cut(s) 152, 212, 680, 718, 723, 2255
HpyF10VI GCNNNNNNNGC 7 cut(s) 206, 285, 729, 826, 946, 1217, 1557
HpyF3I CTNAG 9 cut(s) 575, 762, 816, 1035, 1221, 1466, 1692, 2032, 2187
HpySE526I ACGT 4 cut(s) 464, 613, 1264, 2109
Hsp92II CATG 9 cut(s) 451, 498, 956, 967, 1138, 1164, 1215, 1556, 1838
Ksp22I TGATCA 2 cut(s) 1741, 2272
KspAI GTTAAC 1 cut(s) 2113
KspI CCGCGG 1 cut(s) 1394
LmnI GCTCC 2 cut(s) 592, 946
Lsp1109I GCAGC 7 cut(s) 142, 164, 196, 335, 961, 1066, 2246
LweI GCATC 5 cut(s) 630, 705, 1438, 1767, 2175
MabI ACCWGGT 2 cut(s) 190, 584
MaeI CTAG 6 cut(s) 260, 515, 1313, 1374, 1590, 1782
MaeII ACGT 4 cut(s) 464, 613, 1264, 2109
MaeIII GTNAC 8 cut(s) 388, 629, 685, 1163, 1358, 1409, 1513, 1763
MbiI CCGCTC 1 cut(s) 1935
MflI RGATCY 3 cut(s) 1058, 1268, 2117
MhlI GDGCHC 6 cut(s) 502, 597, 784, 1529, 1756, 2140
MlyI GAGTC 1 cut(s) 97
MmeI TCCRAC 3 cut(s) 570, 846, 871
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 5 cut(s) 570, 1800, 1899, 2112, 2314
MslI CAYNNNNRTG 2 cut(s) 1568, 1605
MspA1I CMGCKG 3 cut(s) 155, 1220, 1393
MspI CCGG 3 cut(s) 1880, 2216, 2232
MspR9I CCNGG 9 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2233, 2289
Mva1269I GAATGC 2 cut(s) 154, 256
MvaI CCWGG 8 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2289
MvnI CGCG 1 cut(s) 1393
MwoI GCNNNNNNNGC 7 cut(s) 206, 285, 729, 826, 946, 1217, 1557
NciI CCSGG 1 cut(s) 2233
NlaIII CATG 9 cut(s) 451, 498, 956, 967, 1138, 1164, 1215, 1556, 1838
NlaIV GGNNCC 7 cut(s) 423, 594, 673, 1060, 2119, 2138, 2139
NmuCI GTSAC 3 cut(s) 388, 1163, 1763
NspI RCATGY 1 cut(s) 1215
NspV TTCGAA 1 cut(s) 1115
PaeI GCATGC 1 cut(s) 1215
PctI GAATGC 2 cut(s) 154, 256
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 7 cut(s) 43, 531, 579, 1442, 1532, 2145, 2199
PflMI CCANNNNNTGG 1 cut(s) 518
PfoI TCCNGGA 1 cut(s) 2064
PinAI ACCGGT 1 cut(s) 1879
PkrI GCNGC 7 cut(s) 154, 157, 211, 325, 951, 1081, 2261
PleI GAGTC 1 cut(s) 96
PpsI GAGTC 1 cut(s) 96
Ppu21I YACGTR 1 cut(s) 1265
Psp6I CCWGG 8 cut(s) 190, 562, 584, 776, 1353, 1997, 2064, 2287
PspEI GGTNACC 1 cut(s) 1358
PspFI CCCAGC 2 cut(s) 275, 2262
PspGI CCWGG 8 cut(s) 190, 562, 584, 776, 1353, 1997, 2064, 2287
PspN4I GGNNCC 7 cut(s) 423, 594, 673, 1060, 2119, 2138, 2139
PspOMI GGGCCC 1 cut(s) 2136
PspPI GGNCC 3 cut(s) 374, 2136, 2137
PsrI GAACNNNNNNTAC 4 cut(s) 1123, 1155, 1842, 1874
PstI CTGCAG 2 cut(s) 214, 682
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 3 cut(s) 1058, 1268, 2117
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 6 cut(s) 662, 839, 975, 1033, 2058, 2293
RsaNI GTAC 6 cut(s) 661, 838, 974, 1032, 2057, 2292
RseI CAYNNNNRTG 2 cut(s) 1568, 1605
SacII CCGCGG 1 cut(s) 1394
SalI GTCGAC 1 cut(s) 103
SaqAI TTAA 5 cut(s) 570, 1800, 1899, 2112, 2314
SatI GCNGC 7 cut(s) 153, 156, 210, 324, 950, 1080, 2260
Sau96I GGNCC 3 cut(s) 374, 2136, 2137
ScaI AGTACT 1 cut(s) 975
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 9 cut(s) 192, 564, 586, 778, 1355, 1999, 2066, 2233, 2289
SduI GDGCHC 6 cut(s) 502, 597, 784, 1529, 1756, 2140
SexAI ACCWGGT 2 cut(s) 190, 584
SfaNI GCATC 5 cut(s) 630, 705, 1438, 1767, 2175
SfcI CTRYAG 2 cut(s) 210, 678
Sfr303I CCGCGG 1 cut(s) 1394
SfuI TTCGAA 1 cut(s) 1115
SgrBI CCGCGG 1 cut(s) 1394
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 1568, 1605
SmlI CTYRAG 1 cut(s) 1323
SmoI CTYRAG 1 cut(s) 1323
SphI GCATGC 1 cut(s) 1215
SsiI CCGC 7 cut(s) 158, 1218, 1391, 1393, 1536, 1933, 2096
SspMI CTAG 6 cut(s) 260, 515, 1313, 1374, 1590, 1782
StyD4I CCNGG 9 cut(s) 190, 562, 584, 776, 1353, 1997, 2064, 2231, 2287
StyI CCWWGG 4 cut(s) 1373, 1478, 1781, 2180
TaaI ACNGT 5 cut(s) 667, 837, 1519, 1758, 1921
TaiI ACGT 4 cut(s) 467, 616, 1267, 2112
TatI WGTACW 2 cut(s) 660, 973
TfiI GAWTC 7 cut(s) 43, 531, 579, 1442, 1532, 2145, 2199
Tru1I TTAA 5 cut(s) 570, 1800, 1899, 2112, 2314
Tru9I TTAA 5 cut(s) 570, 1800, 1899, 2112, 2314
TscAI CASTG 6 cut(s) 130, 219, 682, 725, 739, 1761
TseFI GTSAC 3 cut(s) 388, 1163, 1763
TseI GCWGC 7 cut(s) 152, 155, 209, 323, 949, 1079, 2259
Tsp45I GTSAC 3 cut(s) 388, 1163, 1763
TspDTI ATGAA 8 cut(s) 17, 72, 436, 1151, 1586, 1647, 1653, 2216
TspGWI ACGGA 3 cut(s) 122, 1136, 2130
TspRI CASTG 6 cut(s) 130, 219, 682, 725, 739, 1761
Van91I CCANNNNNTGG 1 cut(s) 518
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 7 cut(s) 299, 379, 434, 1008, 1300, 1730, 1823
XbaI TCTAGA 1 cut(s) 259
XceI RCATGY 1 cut(s) 1215
XmaJI CCTAGG 2 cut(s) 1373, 1781
XmiI GTMKAC 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 6 cut(s) 260, 515, 1313, 1374, 1590, 1782
ZrmI AGTACT 1 cut(s) 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.