RchiOBHm_Chr5g0001351

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000587
Physical Location & Seq
Forward (+)
718105 .. 721336
3232 bp
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UTR
Exon/CDS
Intron
PRQ28279

Sequence Viewer

Length: 1614 bp
ATGGGGCGCAAAGGGAGCGGTGTTGATACAGAAATTCAGGAGAAACCAAGCACATTCTGGACACAGTGTCCCATTTGTTGGGCAAAACTGCAATACTACACGGAGTTTCTGGATCGGTTGCTCCGTTGTCAAATATGCCATAACACATTTCAAGCTCGTGAATTGTTAGAAGATGTTGATGCAGAAGCTCTCAGGAATCAATGTCCAAACCATAAAGAGCCTCCAAAACAGTGTCCACAGGACCCCCCAAACACATTCTGGACACAGTGCTCCTTTTGTTGCGCAAAATTTCAATACTACAGTGATACTCTGGGTCGATTGCTTTGTTGTCAAATATGTCAGAAAGCATTTGAGGCTCATGAATTGAAGAACGATGTTCATCCAGAATTTTTCAGGAATCAATTTTCGAATACTGTGGAGCCTCCAAAACAGGGTCCATCAAACTTAGCCTCAGAAAGTAGTGGTGGAAGTAAAAAGGGAAATATCACAAGATTTCAAGACGGAGATGGAACCTTCAAAAGTTTGAGGAAGGGTCAATCTTCTGGTGTCGCTGAGAAGAAGAGGAATATTGGAGCTGCTGGTGTACATAAGAAAGAGGCAAGAGAAAATGTTAGTTTCCCTCTGGATAAAAATTTTCCAAACAAGAAGAGGAAAACAGAAGAATTTGAGTTAAAAGCAAAGGAAGCCACCATGCTAGATAAAGAGGAAAGCAACTTCAAAGCTGATGTCAGTTCTGCAGCAAATGTAGCCACTCTGGAGAGCATTGAGGTTCCTGATCCTCAATTTCATAAGTTTGTAGTTGATGCAGACATGTTGGCAAATCTTTGTCAAGCTAATCAAACATGGGCTCTTTATGATACAGCAGATGGCATGCCCAGATTCTATGCTATTGTCAAGAAGGTCCTCACCCCTGGATTTAAGCTGAAAGTCAGCTGGCTGGACGCGGATCCAGATGACCAAGGTGAGATTGATTGGTGTGAAAAGGAGTTGCCAGTTGCTTGCGGTAAGTTCAGACTTGGAGACTCTGCAGAAATTACTGATCACCTTATGTTTTCTCATCAGATGCACGCCACAACAGGGAGTCTTACAGGCTCTGTTTTGGTATATCCTAGGAAGGGAGAAACTTGGGCCATCTACCAGAATTGGGATATTGGGTGGAGTTCTGAGCCGGAAAAGCACGTATCTTACAAATATGAATTTGTTGAAGTCTTGTCAGATTATGTGGAAGGTGATGGAATTGGAGTTTCTTATTTAGCCAAAGTAAAAGGATTTGTTAGTTTATTTCAGCAAACCGAGCAGCATGGGTTTCAAGTACCACCTGATGAGCTTTATAGATTTTCTCATCGAATTCCCTCTTTCAAGATGACTGGTCATGAAGGATATGGTGTTCCCTCAGGATCCTTTGAGCTAGATCCTGCTTCTTTGCCCTCCAGTATTTTGATTCATGATGTACGTGATTTGGAAATCGATAACAGAAGGAAGAATACTGAACCTAGTGATTTGTCTGGTATGCAACAGAAGAATATGTGGATTCTAAATGTGAGTTCTATGAAAGAGAAGTGCACAGAAAACTTAAGCCAGGGAAACGTCTCTCAACAAAGTGATATTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

537

Amino Acids

60.71

Weight (kDa)

5.63

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3444 PF11926 253 - 459 2.2e-75 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 283
AccB7I CCANNNNNTGG 1 cut(s) 78
AccBSI CCGCTC 1 cut(s) 18
AccII CGCG 1 cut(s) 944
AciI CCGC 3 cut(s) 18, 944, 1002
AclWI GGATC 7 cut(s) 120, 770, 941, 954, 1392, 1405, 1406
AcsI RAATTY 7 cut(s) 33, 287, 386, 631, 662, 1196, 1347
AfaI GTAC 3 cut(s) 585, 1314, 1452
AfiI CCNNNNNNNGG 5 cut(s) 78, 431, 1077, 1115, 1144
AflII CTTAAG 1 cut(s) 1573
AflIII ACRYGT 1 cut(s) 810
AgsI TTSAA 9 cut(s) 152, 293, 367, 497, 517, 718, 1205, 1310, 1360
AhdI GACNNNNNGTC 1 cut(s) 66
AjnI CCWGG 2 cut(s) 910, 1578
AloI GAACNNNNNNTCC 2 cut(s) 1371, 1403
AluBI AGCT 9 cut(s) 155, 188, 575, 722, 833, 922, 933, 1327, 1408
AluI AGCT 9 cut(s) 155, 188, 575, 722, 833, 922, 933, 1327, 1408
Alw21I GWGCWC 2 cut(s) 272, 1565
Alw26I GTCTC 2 cut(s) 1014, 1594
Alw44I GTGCAC 1 cut(s) 1561
AlwI GGATC 7 cut(s) 120, 770, 941, 954, 1392, 1405, 1406
AlwNI CAGNNNCTG 1 cut(s) 1094
AoxI GGCC 1 cut(s) 1128
ApaLI GTGCAC 1 cut(s) 1561
ApeKI GCWGC 3 cut(s) 575, 737, 1297
ApoI RAATTY 7 cut(s) 33, 287, 386, 631, 662, 1196, 1347
ArsI GACNNNNNNTTYG 2 cut(s) 711, 743
AspA2I CCTAGG 1 cut(s) 1109
AspLEI GCGC 2 cut(s) 9, 284
AspS9I GGNCC 4 cut(s) 241, 434, 901, 1128
AsuHPI GGTGA 4 cut(s) 898, 974, 1034, 1241
AsuII TTCGAA 1 cut(s) 407
AvaII GGWCC 3 cut(s) 241, 434, 901
AvrII CCTAGG 1 cut(s) 1109
AxyI CCTNAGG 1 cut(s) 1393
BaeGI GKGCMC 1 cut(s) 1565
BamHI GGATCC 2 cut(s) 946, 1397
BanII GRGCYC 1 cut(s) 850
BarI GAAGNNNNNNTAC 2 cut(s) 1468, 1500
BauI CACGAG 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 272, 1565
BbvI GCAGC 3 cut(s) 562, 749, 1309
BccI CCATC 5 cut(s) 445, 500, 860, 1139, 1226
BciT130I CCWGG 2 cut(s) 912, 1580
BclI TGATCA 1 cut(s) 1039
BcoDI GTCTC 2 cut(s) 1014, 1594
BfaI CTAG 4 cut(s) 695, 1110, 1409, 1494
BfmI CTRYAG 3 cut(s) 298, 735, 1026
BfrI CTTAAG 1 cut(s) 1573
BisI GCNGC 3 cut(s) 576, 738, 1298
BlnI CCTAGG 1 cut(s) 1109
BlsI GCNGC 3 cut(s) 577, 739, 1299
Bme1390I CCNGG 2 cut(s) 912, 1580
Bme18I GGWCC 3 cut(s) 241, 434, 901
BmeRI GACNNNNNGTC 1 cut(s) 66
BmgT120I GGNCC 4 cut(s) 241, 434, 901, 1128
BmiI GGNNCC 7 cut(s) 243, 420, 435, 511, 771, 948, 1399
BmrFI CCNGG 2 cut(s) 912, 1580
BmsI GCATC 3 cut(s) 169, 793, 1053
BpmI CTGGAG 2 cut(s) 776, 1414
Bpu14I TTCGAA 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 1467
BsaAI YACGTR 2 cut(s) 1180, 1454
BsaBI GATNNNNATC 1 cut(s) 378
BsaJI CCNNGG 4 cut(s) 910, 958, 1109, 1579
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 431, 1077, 1115, 1144
Bse1I ACTGG 3 cut(s) 992, 1372, 1431
Bse21I CCTNAGG 1 cut(s) 1393
Bse8I GATNNNNATC 1 cut(s) 378
BseBI CCWGG 2 cut(s) 912, 1580
BseCI ATCGAT 1 cut(s) 1467
BseDI CCNNGG 4 cut(s) 910, 958, 1109, 1579
BseGI GGATG 1 cut(s) 379
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 5 cut(s) 78, 431, 1077, 1115, 1144
BseMII CTCAG 5 cut(s) 205, 465, 543, 1155, 1407
BseNI ACTGG 3 cut(s) 992, 1372, 1431
BseSI GKGCMC 1 cut(s) 1565
BseXI GCAGC 3 cut(s) 562, 749, 1309
Bsh1236I CGCG 1 cut(s) 944
BshFI GGCC 1 cut(s) 1130
BshVI ATCGAT 1 cut(s) 1467
BsiHKAI GWGCWC 2 cut(s) 272, 1565
BsiSI CCGG 1 cut(s) 1169
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 5 cut(s) 78, 431, 1077, 1115, 1144
BsmAI GTCTC 2 cut(s) 1014, 1594
BsmBI CGTCTC 1 cut(s) 1594
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 1 cut(s) 1130
Bsp119I TTCGAA 1 cut(s) 407
Bsp1286I GDGCHC 3 cut(s) 272, 850, 1565
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
BspACI CCGC 3 cut(s) 18, 944, 1002
BspANI GGCC 1 cut(s) 1130
BspCNI CTCAG 5 cut(s) 204, 464, 544, 1156, 1406
BspDI ATCGAT 1 cut(s) 1467
BspFNI CGCG 1 cut(s) 944
BspHI TCATGA 3 cut(s) 358, 1372, 1444
BspLI GGNNCC 7 cut(s) 243, 420, 435, 511, 771, 948, 1399
BspMAI CTGCAG 2 cut(s) 739, 1030
BspPI GGATC 7 cut(s) 120, 770, 941, 954, 1392, 1405, 1406
BspT104I TTCGAA 1 cut(s) 407
BspTI CTTAAG 1 cut(s) 1573
BsrBI CCGCTC 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 583
BsrI ACTGG 3 cut(s) 992, 1372, 1431
BssECI CCNNGG 4 cut(s) 910, 958, 1109, 1579
BssMI GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 2 cut(s) 958, 1109
Bst2BI CACGAG 1 cut(s) 156
Bst2UI CCWGG 2 cut(s) 912, 1580
Bst4CI ACNGT 5 cut(s) 66, 231, 267, 302, 415
Bst6I CTCTTC 2 cut(s) 554, 641
BstAFI CTTAAG 1 cut(s) 1573
BstAUI TGTACA 1 cut(s) 583
BstBAI YACGTR 2 cut(s) 1180, 1454
BstBI TTCGAA 1 cut(s) 407
BstC8I GCNNGC 4 cut(s) 872, 935, 1000, 1068
BstDEI CTNAG 6 cut(s) 191, 445, 451, 552, 1164, 1393
BstF5I GGATG 1 cut(s) 379
BstFNI CGCG 1 cut(s) 944
BstHHI GCGC 2 cut(s) 9, 284
BstKTI GATC 6 cut(s) 115, 778, 949, 1042, 1400, 1414
BstMAI GTCTC 2 cut(s) 1014, 1594
BstMBI GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
BstMWI GCNNNNNNNGC 6 cut(s) 15, 353, 683, 746, 1174, 1294
BstNI CCWGG 2 cut(s) 912, 1580
BstNSI RCATGY 2 cut(s) 814, 874
BstSCI CCNGG 2 cut(s) 910, 1578
BstSFI CTRYAG 3 cut(s) 298, 735, 1026
BstSLI GKGCMC 1 cut(s) 1565
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 3 cut(s) 562, 749, 1309
BstX2I RGATCY 3 cut(s) 946, 1397, 1411
BstYI RGATCY 3 cut(s) 946, 1397, 1411
Bsu15I ATCGAT 1 cut(s) 1467
Bsu36I CCTNAGG 1 cut(s) 1393
BsuRI GGCC 1 cut(s) 1130
BsuTUI ATCGAT 1 cut(s) 1467
BtsCI GGATG 1 cut(s) 379
BtsIMutI CAGTG 4 cut(s) 71, 236, 272, 307
Cac8I GCNNGC 4 cut(s) 872, 935, 1000, 1068
CaiI CAGNNNCTG 1 cut(s) 1094
CciI TCATGA 3 cut(s) 358, 1372, 1444
CfoI GCGC 2 cut(s) 9, 284
Cfr13I GGNCC 4 cut(s) 241, 434, 901, 1128
ClaI ATCGAT 1 cut(s) 1467
CseI GACGC 1 cut(s) 950
Csp6I GTAC 3 cut(s) 584, 1313, 1451
CviAII CATG 8 cut(s) 359, 691, 811, 843, 871, 1301, 1373, 1445
CviQI GTAC 3 cut(s) 584, 1313, 1451
DdeI CTNAG 6 cut(s) 191, 445, 451, 552, 1164, 1393
DpnI GATC 6 cut(s) 114, 777, 948, 1041, 1399, 1413
DpnII GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
DriI GACNNNNNGTC 1 cut(s) 66
Eam1104I CTCTTC 2 cut(s) 554, 641
Eam1105I GACNNNNNGTC 1 cut(s) 66
EarI CTCTTC 2 cut(s) 554, 641
Eco130I CCWWGG 2 cut(s) 958, 1109
Eco24I GRGCYC 1 cut(s) 850
Eco47I GGWCC 3 cut(s) 241, 434, 901
Eco81I CCTNAGG 1 cut(s) 1393
EcoO109I RGGNCCY 2 cut(s) 241, 901
EcoRI GAATTC 1 cut(s) 1347
EcoRII CCWGG 2 cut(s) 910, 1578
EcoT14I CCWWGG 2 cut(s) 958, 1109
EcoT38I GRGCYC 1 cut(s) 850
ErhI CCWWGG 2 cut(s) 958, 1109
Esp3I CGTCTC 1 cut(s) 1594
FaeI CATG 8 cut(s) 362, 694, 814, 846, 874, 1304, 1376, 1448
FaqI GGGAC 1 cut(s) 54
FatI CATG 8 cut(s) 358, 690, 810, 842, 870, 1300, 1372, 1444
FbaI TGATCA 1 cut(s) 1039
Fnu4HI GCNGC 3 cut(s) 576, 738, 1298
FokI GGATG 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 850
Fsp4HI GCNGC 3 cut(s) 576, 738, 1298
FspBI CTAG 4 cut(s) 695, 1110, 1409, 1494
FspI TGCGCA 1 cut(s) 283
GlaI GCGC 2 cut(s) 8, 283
GluI GCNGC 3 cut(s) 576, 738, 1298
GsuI CTGGAG 2 cut(s) 776, 1414
HaeIII GGCC 1 cut(s) 1130
HapII CCGG 1 cut(s) 1169
HgaI GACGC 1 cut(s) 950
HhaI GCGC 2 cut(s) 9, 284
Hin1II CATG 8 cut(s) 362, 694, 814, 846, 874, 1304, 1376, 1448
Hin6I GCGC 2 cut(s) 7, 282
HinP1I GCGC 2 cut(s) 7, 282
HinfI GANTC 7 cut(s) 196, 397, 879, 1022, 1081, 1441, 1531
HpaII CCGG 1 cut(s) 1169
HphI GGTGA 4 cut(s) 898, 974, 1034, 1241
Hpy166II GTNNAC 3 cut(s) 236, 584, 1563
Hpy188I TCNGA 6 cut(s) 342, 454, 1013, 1062, 1165, 1216
Hpy8I GTNNAC 3 cut(s) 236, 584, 1563
HpyAV CCTTC 7 cut(s) 523, 523, 892, 1108, 1220, 1370, 1470
HpyCH4III ACNGT 5 cut(s) 66, 231, 267, 302, 415
HpyCH4IV ACGT 3 cut(s) 1179, 1453, 1587
HpyCH4V TGCA 8 cut(s) 91, 182, 737, 806, 1028, 1066, 1513, 1563
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 353, 683, 746, 1174, 1294
HpyF3I CTNAG 6 cut(s) 191, 445, 451, 552, 1164, 1393
HpySE526I ACGT 3 cut(s) 1179, 1453, 1587
Hsp92II CATG 8 cut(s) 362, 694, 814, 846, 874, 1304, 1376, 1448
HspAI GCGC 2 cut(s) 7, 282
Ksp22I TGATCA 1 cut(s) 1039
Kzo9I GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
LmnI GCTCC 5 cut(s) 15, 126, 275, 418, 572
Lsp1109I GCAGC 3 cut(s) 562, 749, 1309
LweI GCATC 3 cut(s) 169, 793, 1053
MaeI CTAG 4 cut(s) 695, 1110, 1409, 1494
MaeII ACGT 3 cut(s) 1179, 1453, 1587
MalI GATC 6 cut(s) 114, 777, 948, 1041, 1399, 1413
MbiI CCGCTC 1 cut(s) 18
MboI GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
MboII GAAGA 9 cut(s) 182, 379, 531, 568, 571, 658, 671, 1492, 1531
MflI RGATCY 3 cut(s) 946, 1397, 1411
MhlI GDGCHC 3 cut(s) 272, 850, 1565
MlyI GAGTC 2 cut(s) 1016, 1090
MseI TTAA 3 cut(s) 671, 918, 1574
MspA1I CMGCKG 1 cut(s) 933
MspCI CTTAAG 1 cut(s) 1573
MspI CCGG 1 cut(s) 1169
MspR9I CCNGG 2 cut(s) 912, 1580
MvaI CCWGG 2 cut(s) 912, 1580
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 6 cut(s) 15, 353, 683, 746, 1174, 1294
NdeII GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
NlaIII CATG 8 cut(s) 362, 694, 814, 846, 874, 1304, 1376, 1448
NlaIV GGNNCC 7 cut(s) 243, 420, 435, 511, 771, 948, 1399
NsbI TGCGCA 1 cut(s) 283
NspI RCATGY 2 cut(s) 814, 874
NspV TTCGAA 1 cut(s) 407
PaeI GCATGC 1 cut(s) 874
PagI TCATGA 3 cut(s) 358, 1372, 1444
PciI ACATGT 1 cut(s) 810
PcsI WCGNNNNNNNCGW 1 cut(s) 121
PfeI GAWTC 5 cut(s) 196, 397, 879, 1441, 1531
PflMI CCANNNNNTGG 1 cut(s) 78
PkrI GCNGC 3 cut(s) 577, 739, 1299
PleI GAGTC 2 cut(s) 1016, 1089
PpsI GAGTC 2 cut(s) 1016, 1089
Ppu21I YACGTR 2 cut(s) 1180, 1454
PpuMI RGGWCCY 2 cut(s) 241, 901
PscI ACATGT 1 cut(s) 810
Psp5II RGGWCCY 2 cut(s) 241, 901
Psp6I CCWGG 2 cut(s) 910, 1578
PspGI CCWGG 2 cut(s) 910, 1578
PspN4I GGNNCC 7 cut(s) 243, 420, 435, 511, 771, 948, 1399
PspPI GGNCC 4 cut(s) 241, 434, 901, 1128
PspPPI RGGWCCY 2 cut(s) 241, 901
PstI CTGCAG 2 cut(s) 739, 1030
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 3 cut(s) 946, 1397, 1411
PvuII CAGCTG 1 cut(s) 933
RsaI GTAC 3 cut(s) 585, 1314, 1452
RsaNI GTAC 3 cut(s) 584, 1313, 1451
SaqAI TTAA 3 cut(s) 671, 918, 1574
SatI GCNGC 3 cut(s) 576, 738, 1298
Sau3AI GATC 6 cut(s) 112, 775, 946, 1039, 1397, 1411
Sau96I GGNCC 4 cut(s) 241, 434, 901, 1128
SchI GAGTC 2 cut(s) 1016, 1090
ScrFI CCNGG 2 cut(s) 912, 1580
SduI GDGCHC 3 cut(s) 272, 850, 1565
SfaNI GCATC 3 cut(s) 169, 793, 1053
SfcI CTRYAG 3 cut(s) 298, 735, 1026
SfuI TTCGAA 1 cut(s) 407
SinI GGWCC 3 cut(s) 241, 434, 901
SmlI CTYRAG 1 cut(s) 1573
SmoI CTYRAG 1 cut(s) 1573
SphI GCATGC 1 cut(s) 874
SsiI CCGC 3 cut(s) 18, 944, 1002
SspI AATATT 1 cut(s) 568
SspMI CTAG 4 cut(s) 695, 1110, 1409, 1494
StyD4I CCNGG 2 cut(s) 910, 1578
StyI CCWWGG 2 cut(s) 958, 1109
TaaI ACNGT 5 cut(s) 66, 231, 267, 302, 415
TaiI ACGT 3 cut(s) 1182, 1456, 1590
TaqI TCGA 4 cut(s) 316, 407, 1345, 1467
TatI WGTACW 1 cut(s) 583
TfiI GAWTC 5 cut(s) 196, 397, 879, 1441, 1531
Tru1I TTAA 3 cut(s) 671, 918, 1574
Tru9I TTAA 3 cut(s) 671, 918, 1574
TscAI CASTG 4 cut(s) 71, 236, 272, 307
TseI GCWGC 3 cut(s) 575, 737, 1297
TspDTI ATGAA 7 cut(s) 368, 375, 776, 1209, 1389, 1433, 1565
TspGWI ACGGA 3 cut(s) 113, 116, 516
TspRI CASTG 4 cut(s) 71, 236, 272, 307
Van91I CCANNNNNTGG 1 cut(s) 78
Vha464I CTTAAG 1 cut(s) 1573
VneI GTGCAC 1 cut(s) 1561
VpaK11BI GGWCC 3 cut(s) 241, 434, 901
XapI RAATTY 7 cut(s) 33, 287, 386, 631, 662, 1196, 1347
XceI RCATGY 2 cut(s) 814, 874
XcmI CCANNNNNNNNNTGG 2 cut(s) 54, 255
XmaJI CCTAGG 1 cut(s) 1109
XspI CTAG 4 cut(s) 695, 1110, 1409, 1494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.