RLG00000025533

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000587
Physical Location & Seq
Reverse (-)
46515247 .. 46520388
5142 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025533

Sequence Viewer

Length: 2490 bp
ATGGAGTGCAACAAAGATGAGGCAGAAAGGGCAATGCAACTTTCGGAGAAAAAGATCCAAAATAAGGACTTTAAAGGAGCAAAGGAGATGGCACTAAAGGCTCAACGACTCTACCCTCAGCTTGACAACATTTCTCGCTTAATTACCGTATGTGAAGTTCACTGTTCAGCCGAAAAGAAGGTAGGGGGATCAGAAATGGACTGGTATGGCATTCTTCAGATTCAGCAATTTGATGATGAAGCAACGATCAAGAAACAATACAGAAAACTTGGGCTGCTACTTCATCCCGATAAGAATAGGTTTACTGGGGCGGAGGCTGCTTTTAAGCTGATTGGGGAAGCAAACAGGGTGCTGACTGACCAAGGAAGTCGTTCTAAATATGACATCAAGTTGAGCGCTTTAACTGGTACCAAATCATTGGGTCATCAGTCATATTTTAACTTCTTTGGACCCAAACATTCCAGTGCTGCAAGTAGCGTTCCGAATATTCCTCAGTCACGGTACACTAGTGTGAATTTACATCAGCCGGTGAATGTACAACAACCGGTGCAACCAGACACAATCAAGATAGCTTGCCCCTCGTGTAAAAGAATGTACGATTACAACTTTGCAAATCGACTCATATTTTGTCAAAGATGCCTAGGAATCTTTGATGCCTCTGATTTGGGTCCAGAATCTCTTGGGAATCCAGTAGCTCCAAGTCAAGGTTCTTCAAAAGAAGCTTCACAAAGTAATGGAGGGACTGGATACCCATCTTCAACAACTAATGGAAATGCAACGTCAAAGGCAGCAAGTGCTACTGAAGGTGGTGGAGCCTCTAAGACTGTGAAGAGAGATATCGAGAATGGAGTTGGAATTGATAAGCAAGGGGGTGAAAGGTCCGAGTCTGGTCCACTGAAGTCAAAGAATTCAAGAAAATCAAGAAACAGAAAAAGAGGAGAGAATGCAACTTTTGATTCTGGTGAAAGTTCCAAGACTGGAAAAGAAAAGGTTGGTACTCCTTCTGAAGTCAGTGAGCATCACACCAGGAGATCTTCAAGGAGTATGCATAAACATTCCTTTTATGAAACTGTAAATGATGATGATGATGATGAAAATGATGATGATGTTGGCAATGTCAAGGTGAATGATGATGTCAATGTGAGTCCTTCGAAAGTGAGGGAAAGCCCATTTCCCACTGCTACTAGAAAGAAGAGGGTTAATGCATCTGCGGATGGTGGAGTGTATAGGCAAACTGTCTTGGCCCTTTGTGCTATGGATGAGTATAGGAAATTAGGAAAACAAAGAGTGAGGCGCAAAAGAAGCAACACTGATGAAATTGAGTTACAGGTAAATGAATTATCCATGGCAGATAACAGTGGCAGCAAGTCCGCGGCAGATAACAGTGGCAGCAAGTCCGCGGCAGATAACAGTGGCAGCAAGTCCGCGGCAGATAACAGTGGCAGCAAGTCCGCGGCAGATAACAGTGGCAGCAAGTCCGCGGCAGATAACAATGGCAGCAAGTCCGCGGCAGATAACAGTGGCAGCAAGTCCGTGGCAGATAACAGTGGCAGCAAGTCCATGACATCCATGCGAAAGATCATTGAGGTTCCTGAACCTGAGTTCCATAGGTTTGTGCTCAGTAAAGATGTGTTACAGAAAACTTTTAAAGCCAATCAAACATGGGTTCTTTATGATCCAGCAGATGGCATGCCAAGACGCTATGCATGTATCAAGAAAGTCTTACACCCTGGATTTAGGGTGAAAATCACCTGGCTGGAGCCCGATCCCGATGACCAAGGTGAGGTTGATTGGTGTAGCAAAAAGTTGCCAGTTGCATGTGGTAAGTTTGGACTTGCGCTCACTGAAGAAGTTGCAGATCATACTGTGTTTTCTCATGAGTATATACACTCCACAAAAGGCAAGGATAAAAGCTCTCTTCGGATATATCCTAGGAAGGGAGAAACCTGGGCCCTCTACCGGGATTGGGATTTGGGGTGGAGTTCTGAGCCTGAAAAGCATTCACAATACAAGTTTGATTTTGTAGAAGTCCTGTCAGATTTTTCTGAAAAGTTTGGCGCTGGAGTTGCTTATTTAGGCAAAGTAAAAGGATTTGTCAGTTTGTTTGAGCCAGCTGAGCAGCAGGGGGTTCTCAAGTTTCAAGTTCCGCCTAATGAGTTGTATAGATTTTCTCATCAAATTCCCTGTTACAAGATGTGTGGTAGTGAAGGATATGGTGTTCCTGCTGGTTCTTTTGAGTTTGATCCTGCTTCTTTGCCTGCCAGTCTTTTCGATCGAAGAAGCACTGTCACCATAGCCAAACCTTCTGGCTTCATACCTTCTGGTAGAGCTCGCCCTGCTAAGAAAGAGCACATCATCATCATCAGGTTTGAGAGCTCGCGTTTAGGCTTCGGTGTTGGGTGGTGCTTTTGTGTCGATGGGGATCTCCTGAATCTCTTGGTTGATTGCTTTTGCTCATACCTTTACCACATCCCCTTACTGAATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

830

Amino Acids

91.2

Weight (kDa)

8.74

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 128 1.3e-17 DnaJ domain
DUF3444 PF11926 526 - 736 6.3e-72 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 407
AccB1I GGYRCC 1 cut(s) 407
AccB7I CCANNNNNTGG 1 cut(s) 1685
AccII CGCG 7 cut(s) 1373, 1400, 1427, 1454, 1481, 1508, 2380
AclWI GGATC 6 cut(s) 49, 196, 1670, 1760, 2237, 2430
AcsI RAATTY 3 cut(s) 514, 907, 2178
AcuI CTGAAG 5 cut(s) 200, 822, 917, 1026, 1866
AfaI GTAC 5 cut(s) 409, 503, 537, 596, 997
AfeI AGCGCT 1 cut(s) 397
AfiI CCNNNNNNNGG 8 cut(s) 64, 704, 1685, 1783, 1937, 1959, 1960, 1966
AgeI ACCGGT 1 cut(s) 544
AgsI TTSAA 5 cut(s) 714, 759, 912, 1038, 2141
AhlI ACTAGT 1 cut(s) 506
AjnI CCWGG 4 cut(s) 1025, 1729, 1751, 1946
AjuI GAANNNNNNNTTGG 2 cut(s) 691, 723
AleI CACNNNNGTG 1 cut(s) 509
AloI GAACNNNNNNTCC 4 cut(s) 1587, 1619, 2202, 2234
AluBI AGCT 9 cut(s) 121, 328, 572, 695, 722, 1914, 2114, 2330, 2376
AluI AGCT 9 cut(s) 121, 328, 572, 695, 722, 1914, 2114, 2330, 2376
Alw21I GWGCWC 4 cut(s) 1620, 2332, 2352, 2378
AlwI GGATC 6 cut(s) 49, 196, 1670, 1760, 2237, 2430
Aor51HI AGCGCT 1 cut(s) 397
AoxI GGCC 2 cut(s) 1242, 1950
ApaI GGGCCC 1 cut(s) 1954
ApoI RAATTY 3 cut(s) 514, 907, 2178
ArsI GACNNNNNNTTYG 2 cut(s) 609, 641
AsiGI ACCGGT 1 cut(s) 544
Asp700I GAANNNNTTC 1 cut(s) 370
Asp718I GGTACC 1 cut(s) 407
AspA2I CCTAGG 2 cut(s) 640, 1931
AspLEI GCGC 4 cut(s) 398, 1296, 1840, 2060
AspS9I GGNCC 7 cut(s) 449, 668, 879, 890, 1243, 1950, 1951
AsuC2I CCSGG 1 cut(s) 1961
AsuHPI GGTGA 8 cut(s) 541, 884, 974, 1135, 1741, 1753, 1793, 2281
AsuII TTCGAA 1 cut(s) 1151
AvaII GGWCC 4 cut(s) 449, 668, 879, 890
AvrII CCTAGG 2 cut(s) 640, 1931
BaeGI GKGCMC 1 cut(s) 1954
BaeI ACNNNNGTAYC 2 cut(s) 493, 526
BanI GGYRCC 1 cut(s) 407
BanII GRGCYC 4 cut(s) 1764, 1954, 2332, 2378
BauI CACGAG 1 cut(s) 580
Bbv12I GWGCWC 4 cut(s) 1620, 2332, 2352, 2378
BbvCI CCTCAGC 1 cut(s) 117
BccI CCATC 5 cut(s) 82, 760, 1208, 1679, 2411
BciT130I CCWGG 4 cut(s) 1027, 1731, 1753, 1948
BciVI GTATCC 1 cut(s) 740
BcnI CCSGG 1 cut(s) 1961
BcuI ACTAGT 1 cut(s) 506
BfaI CTAG 4 cut(s) 507, 641, 1185, 1932
BfoI RGCGCY 2 cut(s) 399, 2061
BfuI GTATCC 1 cut(s) 740
BglII AGATCT 1 cut(s) 1031
BlnI CCTAGG 2 cut(s) 640, 1931
BlpI GCTNAGC 1 cut(s) 2115
Bme1390I CCNGG 5 cut(s) 1027, 1731, 1753, 1948, 1961
Bme18I GGWCC 4 cut(s) 449, 668, 879, 890
BmgT120I GGNCC 7 cut(s) 449, 668, 879, 890, 1243, 1950, 1951
BmiI GGNNCC 7 cut(s) 409, 451, 669, 814, 1590, 1761, 1952
BmrFI CCNGG 5 cut(s) 1027, 1731, 1753, 1948, 1961
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 4 cut(s) 626, 643, 1027, 1214
BmuI ACTGGG 1 cut(s) 315
BpmI CTGGAG 2 cut(s) 1778, 2082
Bpu10I CCTNAGC 1 cut(s) 117
Bpu1102I GCTNAGC 1 cut(s) 2115
Bpu14I TTCGAA 1 cut(s) 1151
BpuEI CTTGAG 1 cut(s) 2117
BpuMI CCSGG 1 cut(s) 1961
BsaBI GATNNNNATC 2 cut(s) 751, 2421
BsaWI WCCGGW 1 cut(s) 544
Bsc4I CCNNNNNNNGG 8 cut(s) 64, 704, 1685, 1783, 1937, 1959, 1960, 1966
Bse118I RCCGGY 2 cut(s) 526, 544
Bse1I ACTGG 9 cut(s) 206, 310, 409, 462, 689, 748, 982, 1811, 2262
Bse3DI GCAATG 2 cut(s) 39, 1120
Bse8I GATNNNNATC 2 cut(s) 751, 2421
BseBI CCWGG 4 cut(s) 1027, 1731, 1753, 1948
BseGI GGATG 5 cut(s) 283, 1219, 1264, 1565, 2469
BseJI GATNNNNATC 2 cut(s) 751, 2421
BseLI CCNNNNNNNGG 8 cut(s) 64, 704, 1685, 1783, 1937, 1959, 1960, 1966
BseMI GCAATG 2 cut(s) 39, 1120
BseMII CTCAG 6 cut(s) 131, 506, 1590, 1633, 1977, 2106
BseNI ACTGG 9 cut(s) 206, 310, 409, 462, 689, 748, 982, 1811, 2262
BseRI GAGGAG 1 cut(s) 951
BseSI GKGCMC 1 cut(s) 1954
Bsh1236I CGCG 7 cut(s) 1373, 1400, 1427, 1454, 1481, 1508, 2380
Bsh1285I CGRYCG 1 cut(s) 2275
BshFI GGCC 2 cut(s) 1244, 1952
BshNI GGYRCC 1 cut(s) 407
BshTI ACCGGT 1 cut(s) 544
BsiEI CGRYCG 1 cut(s) 2275
BsiHKAI GWGCWC 4 cut(s) 1620, 2332, 2352, 2378
BsiSI CCGG 3 cut(s) 527, 545, 1960
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 8 cut(s) 64, 704, 1685, 1783, 1937, 1959, 1960, 1966
BsmFI GGGAC 1 cut(s) 754
BsmI GAATGC 3 cut(s) 210, 949, 1999
BsnI GGCC 2 cut(s) 1244, 1952
Bsp119I TTCGAA 1 cut(s) 1151
Bsp120I GGGCCC 1 cut(s) 1950
Bsp1286I GDGCHC 6 cut(s) 1620, 1764, 1954, 2332, 2352, 2378
Bsp1407I TGTACA 1 cut(s) 535
Bsp1720I GCTNAGC 1 cut(s) 2115
Bsp19I CCATGG 1 cut(s) 1344
BspANI GGCC 2 cut(s) 1244, 1952
BspCNI CTCAG 6 cut(s) 130, 505, 1591, 1632, 1978, 2107
BspFNI CGCG 7 cut(s) 1373, 1400, 1427, 1454, 1481, 1508, 2380
BspHI TCATGA 1 cut(s) 1876
BspLI GGNNCC 7 cut(s) 409, 451, 669, 814, 1590, 1761, 1952
BspPI GGATC 6 cut(s) 49, 196, 1670, 1760, 2237, 2430
BspT104I TTCGAA 1 cut(s) 1151
BspT107I GGYRCC 1 cut(s) 407
BsrDI GCAATG 2 cut(s) 39, 1120
BsrFI RCCGGY 2 cut(s) 526, 544
BsrGI TGTACA 1 cut(s) 535
BsrI ACTGG 9 cut(s) 206, 310, 409, 462, 689, 748, 982, 1811, 2262
BssAI RCCGGY 2 cut(s) 526, 544
BssSI CACGAG 1 cut(s) 580
BssT1I CCWWGG 5 cut(s) 361, 640, 1344, 1777, 1931
Bst2BI CACGAG 1 cut(s) 580
Bst2UI CCWGG 4 cut(s) 1027, 1731, 1753, 1948
Bst6I CTCTTC 3 cut(s) 824, 1187, 1923
BstAPI GCANNNNNTGC 1 cut(s) 794
BstAUI TGTACA 1 cut(s) 535
BstBI TTCGAA 1 cut(s) 1151
BstC8I GCNNGC 6 cut(s) 574, 1691, 2112, 2259, 2332, 2378
BstDEI CTNAG 8 cut(s) 117, 492, 819, 1599, 1619, 1986, 2115, 2340
BstDSI CCRYGG 8 cut(s) 1344, 1371, 1398, 1425, 1452, 1479, 1506, 1533
BstF5I GGATG 5 cut(s) 283, 1219, 1264, 1565, 2469
BstFNI CGCG 7 cut(s) 1373, 1400, 1427, 1454, 1481, 1508, 2380
BstH2I RGCGCY 2 cut(s) 399, 2061
BstHHI GCGC 4 cut(s) 398, 1296, 1840, 2060
BstMCI CGRYCG 1 cut(s) 2275
BstNI CCWGG 4 cut(s) 1027, 1731, 1753, 1948
BstNSI RCATGY 3 cut(s) 1693, 1710, 1821
BstSCI CCNGG 5 cut(s) 1025, 1729, 1751, 1946, 1959
BstSLI GKGCMC 1 cut(s) 1954
BstUI CGCG 7 cut(s) 1373, 1400, 1427, 1454, 1481, 1508, 2380
BstX2I RGATCY 3 cut(s) 54, 1031, 2422
BstXI CCANNNNNNTGG 1 cut(s) 418
BstYI RGATCY 3 cut(s) 54, 1031, 2422
BsuI GTATCC 1 cut(s) 740
BsuRI GGCC 2 cut(s) 1244, 1952
BtgI CCRYGG 8 cut(s) 1344, 1371, 1398, 1425, 1452, 1479, 1506, 1533
BtsCI GGATG 5 cut(s) 283, 1219, 1264, 1565, 2469
BtsI GCAGTG 1 cut(s) 1176
Cac8I GCNNGC 6 cut(s) 574, 1691, 2112, 2259, 2332, 2378
CciI TCATGA 1 cut(s) 1876
CfoI GCGC 4 cut(s) 398, 1296, 1840, 2060
Cfr10I RCCGGY 2 cut(s) 526, 544
Cfr13I GGNCC 7 cut(s) 449, 668, 879, 890, 1243, 1950, 1951
Cfr42I CCGCGG 6 cut(s) 1374, 1401, 1428, 1455, 1482, 1509
CseI GACGC 1 cut(s) 1707
Csp6I GTAC 5 cut(s) 408, 502, 536, 595, 996
CspAI ACCGGT 1 cut(s) 544
CspCI CAANNNNNGTGG 2 cut(s) 2179, 2214
CviAII CATG 8 cut(s) 1345, 1561, 1570, 1662, 1690, 1707, 1818, 1877
CviQI GTAC 5 cut(s) 408, 502, 536, 595, 996
DdeI CTNAG 8 cut(s) 117, 492, 819, 1599, 1619, 1986, 2115, 2340
DraI TTTAAA 2 cut(s) 73, 1648
Eam1104I CTCTTC 3 cut(s) 824, 1187, 1923
EarI CTCTTC 3 cut(s) 824, 1187, 1923
EciI GGCGGA 2 cut(s) 326, 2136
Ecl136II GAGCTC 2 cut(s) 2330, 2376
Eco130I CCWWGG 5 cut(s) 361, 640, 1344, 1777, 1931
Eco24I GRGCYC 4 cut(s) 1764, 1954, 2332, 2378
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 4 cut(s) 449, 668, 879, 890
Eco47III AGCGCT 1 cut(s) 397
Eco53kI GAGCTC 2 cut(s) 2330, 2376
Eco57I CTGAAG 5 cut(s) 200, 822, 917, 1026, 1866
EcoICRI GAGCTC 2 cut(s) 2330, 2376
EcoO109I RGGNCCY 1 cut(s) 1951
EcoRI GAATTC 1 cut(s) 907
EcoRII CCWGG 4 cut(s) 1025, 1729, 1751, 1946
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 5 cut(s) 361, 640, 1344, 1777, 1931
EcoT22I ATGCAT 3 cut(s) 1050, 1207, 1708
EcoT38I GRGCYC 4 cut(s) 1764, 1954, 2332, 2378
ErhI CCWWGG 5 cut(s) 361, 640, 1344, 1777, 1931
FaeI CATG 8 cut(s) 1348, 1564, 1573, 1665, 1693, 1710, 1821, 1880
FalI AAGNNNNNCTT 2 cut(s) 1706, 1738
FaqI GGGAC 1 cut(s) 754
FatI CATG 8 cut(s) 1344, 1560, 1569, 1661, 1689, 1706, 1817, 1876
FokI GGATG 5 cut(s) 270, 1226, 1271, 1552, 2456
FriOI GRGCYC 4 cut(s) 1764, 1954, 2332, 2378
FspBI CTAG 4 cut(s) 507, 641, 1185, 1932
GlaI GCGC 4 cut(s) 397, 1295, 1839, 2059
GsuI CTGGAG 2 cut(s) 1778, 2082
HaeII RGCGCY 2 cut(s) 399, 2061
HaeIII GGCC 2 cut(s) 1244, 1952
HapII CCGG 3 cut(s) 527, 545, 1960
HgaI GACGC 1 cut(s) 1707
HhaI GCGC 4 cut(s) 398, 1296, 1840, 2060
Hin1II CATG 8 cut(s) 1348, 1564, 1573, 1665, 1693, 1710, 1821, 1880
Hin6I GCGC 4 cut(s) 396, 1294, 1838, 2058
HinP1I GCGC 4 cut(s) 396, 1294, 1838, 2058
HindIII AAGCTT 1 cut(s) 720
HpaII CCGG 3 cut(s) 527, 545, 1960
HphI GGTGA 8 cut(s) 541, 884, 974, 1135, 1741, 1753, 1793, 2281
Hpy166II GTNNAC 4 cut(s) 160, 303, 504, 893
Hpy8I GTNNAC 4 cut(s) 160, 303, 504, 893
HpyAV CCTTC 8 cut(s) 172, 797, 1011, 1158, 1930, 2201, 2313, 2328
HpyCH4IV ACGT 1 cut(s) 779
HpyF3I CTNAG 8 cut(s) 117, 492, 819, 1599, 1619, 1986, 2115, 2340
HpySE526I ACGT 1 cut(s) 779
Hsp92II CATG 8 cut(s) 1348, 1564, 1573, 1665, 1693, 1710, 1821, 1880
HspAI GCGC 4 cut(s) 396, 1294, 1838, 2058
KpnI GGTACC 1 cut(s) 411
KspI CCGCGG 6 cut(s) 1374, 1401, 1428, 1455, 1482, 1509
LmnI GCTCC 4 cut(s) 77, 700, 812, 1759
LweI GCATC 4 cut(s) 626, 643, 1027, 1214
MaeI CTAG 4 cut(s) 507, 641, 1185, 1932
MaeII ACGT 1 cut(s) 779
MaeIII GTNAC 5 cut(s) 495, 1323, 1632, 2186, 2287
MboII GAAGA 9 cut(s) 206, 702, 747, 841, 1026, 1204, 1859, 1910, 2289
MflI RGATCY 3 cut(s) 54, 1031, 2422
MhlI GDGCHC 6 cut(s) 1620, 1764, 1954, 2332, 2352, 2378
MluCI AATT 9 cut(s) 141, 227, 514, 855, 907, 1271, 1317, 1337, 2178
MlyI GAGTC 4 cut(s) 102, 612, 893, 1153
MmeI TCCRAC 1 cut(s) 832
Mph1103I ATGCAT 3 cut(s) 1050, 1207, 1708
MroXI GAANNNNTTC 1 cut(s) 370
MseI TTAA 7 cut(s) 72, 140, 324, 401, 438, 1200, 1647
MslI CAYNNNNRTG 2 cut(s) 462, 509
MspA1I CMGCKG 7 cut(s) 1373, 1400, 1427, 1454, 1481, 1508, 2114
MspI CCGG 3 cut(s) 527, 545, 1960
MspR9I CCNGG 5 cut(s) 1027, 1731, 1753, 1948, 1961
Mva1269I GAATGC 3 cut(s) 210, 949, 1999
MvaI CCWGG 4 cut(s) 1027, 1731, 1753, 1948
MvnI CGCG 7 cut(s) 1373, 1400, 1427, 1454, 1481, 1508, 2380
NciI CCSGG 1 cut(s) 1961
NcoI CCATGG 1 cut(s) 1344
NlaIII CATG 8 cut(s) 1348, 1564, 1573, 1665, 1693, 1710, 1821, 1880
NlaIV GGNNCC 7 cut(s) 409, 451, 669, 814, 1590, 1761, 1952
NmuCI GTSAC 2 cut(s) 495, 2287
NsiI ATGCAT 3 cut(s) 1050, 1207, 1708
NspI RCATGY 3 cut(s) 1693, 1710, 1821
NspV TTCGAA 1 cut(s) 1151
OliI CACNNNNGTG 1 cut(s) 509
PaeI GCATGC 1 cut(s) 1693
PagI TCATGA 1 cut(s) 1876
PctI GAATGC 3 cut(s) 210, 949, 1999
PdmI GAANNNNTTC 1 cut(s) 370
PfeI GAWTC 6 cut(s) 220, 645, 674, 685, 956, 2431
PflMI CCANNNNNTGG 1 cut(s) 1685
PinAI ACCGGT 1 cut(s) 544
Ple19I CGATCG 1 cut(s) 2275
PleI GAGTC 4 cut(s) 102, 612, 892, 1152
PpsI GAGTC 4 cut(s) 102, 612, 892, 1152
Psp124BI GAGCTC 2 cut(s) 2332, 2378
Psp6I CCWGG 4 cut(s) 1025, 1729, 1751, 1946
PspGI CCWGG 4 cut(s) 1025, 1729, 1751, 1946
PspN4I GGNNCC 7 cut(s) 409, 451, 669, 814, 1590, 1761, 1952
PspOMI GGGCCC 1 cut(s) 1950
PspPI GGNCC 7 cut(s) 449, 668, 879, 890, 1243, 1950, 1951
PsrI GAACNNNNNNTAC 2 cut(s) 141, 173
PsuI RGATCY 3 cut(s) 54, 1031, 2422
PvuI CGATCG 1 cut(s) 2275
PvuII CAGCTG 1 cut(s) 2114
RsaI GTAC 5 cut(s) 409, 503, 537, 596, 997
RsaNI GTAC 5 cut(s) 408, 502, 536, 595, 996
RseI CAYNNNNRTG 2 cut(s) 462, 509
SacI GAGCTC 2 cut(s) 2332, 2378
SacII CCGCGG 6 cut(s) 1374, 1401, 1428, 1455, 1482, 1509
SaqAI TTAA 7 cut(s) 72, 140, 324, 401, 438, 1200, 1647
Sau96I GGNCC 7 cut(s) 449, 668, 879, 890, 1243, 1950, 1951
SchI GAGTC 4 cut(s) 102, 612, 893, 1153
ScrFI CCNGG 5 cut(s) 1027, 1731, 1753, 1948, 1961
SduI GDGCHC 6 cut(s) 1620, 1764, 1954, 2332, 2352, 2378
SfaNI GCATC 4 cut(s) 626, 643, 1027, 1214
Sfr303I CCGCGG 6 cut(s) 1374, 1401, 1428, 1455, 1482, 1509
SfuI TTCGAA 1 cut(s) 1151
SgrBI CCGCGG 6 cut(s) 1374, 1401, 1428, 1455, 1482, 1509
SinI GGWCC 4 cut(s) 449, 668, 879, 890
SmiMI CAYNNNNRTG 2 cut(s) 462, 509
SmlI CTYRAG 1 cut(s) 2132
SmoI CTYRAG 1 cut(s) 2132
SpeI ACTAGT 1 cut(s) 506
SphI GCATGC 1 cut(s) 1693
Sse9I AATT 9 cut(s) 141, 227, 514, 855, 907, 1271, 1317, 1337, 2178
SspI AATATT 1 cut(s) 487
SspMI CTAG 4 cut(s) 507, 641, 1185, 1932
SstI GAGCTC 2 cut(s) 2332, 2378
StyD4I CCNGG 5 cut(s) 1025, 1729, 1751, 1946, 1959
StyI CCWWGG 5 cut(s) 361, 640, 1344, 1777, 1931
TaiI ACGT 1 cut(s) 782
TaqI TCGA 6 cut(s) 616, 840, 1151, 2271, 2275, 2415
TasI AATT 9 cut(s) 141, 227, 514, 855, 907, 1271, 1317, 1337, 2178
TatI WGTACW 1 cut(s) 535
TauI GCSGC 6 cut(s) 1376, 1403, 1430, 1457, 1484, 1511
TfiI GAWTC 6 cut(s) 220, 645, 674, 685, 956, 2431
Tru1I TTAA 7 cut(s) 72, 140, 324, 401, 438, 1200, 1647
Tru9I TTAA 7 cut(s) 72, 140, 324, 401, 438, 1200, 1647
TseFI GTSAC 2 cut(s) 495, 2287
Tsp45I GTSAC 2 cut(s) 495, 2287
TspDTI ATGAA 7 cut(s) 252, 272, 1080, 1107, 1329, 1350, 2302
TspGWI ACGGA 1 cut(s) 1522
Van91I CCANNNNNTGG 1 cut(s) 1685
VpaK11BI GGWCC 4 cut(s) 449, 668, 879, 890
XapI RAATTY 3 cut(s) 514, 907, 2178
XceI RCATGY 3 cut(s) 1693, 1710, 1821
XmaJI CCTAGG 2 cut(s) 640, 1931
XmnI GAANNNNTTC 1 cut(s) 370
XspI CTAG 4 cut(s) 507, 641, 1185, 1932
Zsp2I ATGCAT 3 cut(s) 1050, 1207, 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.