Rw3G003830

Domain of unknown function (DUF3444)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000587
Physical Location & Seq
Forward (+)
3294508 .. 3299654
5147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003830.1

Sequence Viewer

Length: 1359 bp
ATGGACTGCAACAAAGAGGAGGCTATCAGAGCAATGCAGCTGTCAGAAGAAAAGATGCAGCTTAATGATTTTGCAGGAGCAAGGAAGTTGGCTCAAACAGTTCAACGCCTCTGCCCTGAGCTTCAGAACATTTCTCAGCTACTAACAGCCTGTGAGGTTCACTGTTCAGCAGAAAACAAGATAGCTGGATGTGGAATGGACTGGTATGGGATTCTTCAGGTTCAGCAATCTGATAATGATGAGAGCATAAAGAAACAATTCAGAAAACTAGCGCTATTACTTCATCCTGATAAGAATAAATTTGCTGGAGCTGAGGCTGCTTTTAAGCTGATCATGGATGCAAACAGGGTGCTGGCGGACCAAGGAACTCGTTCTAAGTATGACAAGAAGTATAGAAAAGCTTTAGCAAAAGCTGGTAGAATTGGAAGCTCTTCTGTACCAATACACAAAGGAAATGCAACCTTGAATCCGGAGTCAAAGGCAGGATTTGCTGCTGATGTTGGGACTTCTAACACTGTAAAGAAAGATATTGGGCATGCAGATGGAGTAGGCAAGCAAGGTGTTGATTCTTCCAGGTCTGGTCCAAGGTCCAAGGATCTAGGAACTTTGAGAAATATGAATAAAAAGAGAGGAAGAAGTTCAAGTTTTGAATGCACTGAAAATTCCAACAGAGGAAAGAGAGCTAGAGCTGATTCTGAGCTGAATGGAGGTGATCACCTTAGGAGATCTTCAAGGAGCAAGCAGAATCTTTCGTCCAGTGAAAAGCTAAATAATAATGGTGCTATGAATGGTGGGATATTAAAAAATGATAGGCCAACTGATTCTGGAGCCAATTCAGTTGTTGCATCCGTACCAAAGGTCTTTGAGGTTCCTGAACCTGAATTCAATAAGTTTGCGCCCGAGGGAAATATTTTTAATGCTAATCAAACATGGGCACTTTATGATCAAGTAGACGGCATGCCAAGGTTCAATGCTCGAGTTAAGAAAGTGCACACTCCCGGATTTAAGGTGAAAATCACCTGGCTCATTCCCAATCCAGATGACAAAGGTGAGATTGGTTGGGTTAAAGAAGACTTGCCAGTTGCTTGTGGTAAGTACACAATTGGGCTCACTGAAGACATTACAGATCATCTATCGCTAATAGTTTTTCAGTTTTATCTTGGGAAATTCTTAAAATGGCAGACCAAAATGGGCCACTTACAAAAATGGTATTTCGTGATTGATGCTTCATCCCCGGTTTGGCCTTATGTACTTGTGGGTGGACACATGGATGGAGCTCTGCTGAACCTTTTAGTGGGGAAAAGCCAGATTGTTGCTCCAAGTATTTTTACATGCATCTTCGATTTTGATACTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3444 DnaJ

Protein Analysis

452

Amino Acids

49.89

Weight (kDa)

9.22

Isoelectric Point (pI)

44.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 67 - 128 2.2e-18 DnaJ domain
DUF3444 PF11926 286 - 378 1.3e-25 Domain of unknown function (DUF3444)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 951
AccIII TCCGGA 1 cut(s) 469
AciI CCGC 1 cut(s) 356
AclWI GGATC 1 cut(s) 603
AcsI RAATTY 4 cut(s) 299, 661, 881, 1166
AcuI CTGAAG 3 cut(s) 107, 200, 1134
AfaI GTAC 4 cut(s) 438, 852, 1097, 1251
AfeI AGCGCT 1 cut(s) 273
AfiI CCNNNNNNNGG 2 cut(s) 1239, 1294
AgsI TTSAA 7 cut(s) 104, 466, 642, 650, 732, 886, 970
AjnI CCWGG 2 cut(s) 572, 1019
Alw21I GWGCWC 2 cut(s) 993, 1279
Alw44I GTGCAC 1 cut(s) 989
AlwI GGATC 1 cut(s) 603
Ama87I CYCGRG 2 cut(s) 899, 975
Aor13HI TCCGGA 1 cut(s) 469
Aor51HI AGCGCT 1 cut(s) 273
AoxI GGCC 3 cut(s) 812, 1192, 1241
ApaLI GTGCAC 1 cut(s) 989
ApeKI GCWGC 4 cut(s) 37, 58, 317, 491
ApoI RAATTY 4 cut(s) 299, 661, 881, 1166
Asp700I GAANNNNTTC 4 cut(s) 257, 370, 430, 637
AspLEI GCGC 2 cut(s) 274, 898
AspS9I GGNCC 4 cut(s) 358, 581, 588, 1192
AsuC2I CCSGG 2 cut(s) 999, 1235
AsuHPI GGTGA 5 cut(s) 707, 722, 1009, 1021, 1061
AvaI CYCGRG 2 cut(s) 899, 975
AvaII GGWCC 3 cut(s) 358, 581, 588
AxyI CCTNAGG 1 cut(s) 719
BaeGI GKGCMC 2 cut(s) 937, 993
BanII GRGCYC 2 cut(s) 1110, 1279
BbsI GAAGAC 2 cut(s) 1077, 1122
Bbv12I GWGCWC 2 cut(s) 993, 1279
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 4 cut(s) 49, 70, 304, 478
BccI CCATC 2 cut(s) 536, 1265
BceAI ACGGC 1 cut(s) 970
BciT130I CCWGG 2 cut(s) 574, 1021
BclI TGATCA 3 cut(s) 330, 712, 943
BcnI CCSGG 2 cut(s) 999, 1235
BfaI CTAG 3 cut(s) 269, 599, 684
BfoI RGCGCY 1 cut(s) 275
BglII AGATCT 1 cut(s) 725
BisI GCNGC 4 cut(s) 38, 59, 318, 492
BlsI GCNGC 4 cut(s) 39, 60, 319, 493
Bme1390I CCNGG 4 cut(s) 574, 999, 1021, 1235
Bme18I GGWCC 3 cut(s) 358, 581, 588
BmeT110I CYCGRG 2 cut(s) 899, 975
BmgT120I GGNCC 4 cut(s) 358, 581, 588, 1192
BmiI GGNNCC 2 cut(s) 829, 870
BmrFI CCNGG 4 cut(s) 574, 999, 1021, 1235
BmsI GCATC 5 cut(s) 45, 328, 854, 1213, 1344
BpiI GAAGAC 2 cut(s) 1077, 1122
BpmI CTGGAG 2 cut(s) 327, 846
Bpu10I CCTNAGC 2 cut(s) 117, 312
BpuMI CCSGG 2 cut(s) 999, 1235
BsaJI CCNNGG 6 cut(s) 361, 584, 591, 900, 962, 1233
BsaWI WCCGGW 1 cut(s) 469
Bsc4I CCNNNNNNNGG 2 cut(s) 1239, 1294
Bse1I ACTGG 3 cut(s) 206, 756, 1079
Bse21I CCTNAGG 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 39
BseAI TCCGGA 1 cut(s) 469
BseBI CCWGG 2 cut(s) 574, 1021
BseDI CCNNGG 6 cut(s) 361, 584, 591, 900, 962, 1233
BseGI GGATG 6 cut(s) 194, 283, 343, 845, 1229, 1276
BseLI CCNNNNNNNGG 2 cut(s) 1239, 1294
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 4 cut(s) 108, 149, 303, 687
BseNI ACTGG 3 cut(s) 206, 756, 1079
BseRI GAGGAG 1 cut(s) 32
BseSI GKGCMC 2 cut(s) 937, 993
BseXI GCAGC 4 cut(s) 49, 70, 304, 478
BshFI GGCC 3 cut(s) 814, 1194, 1243
BsiHKAI GWGCWC 2 cut(s) 993, 1279
BsiHKCI CYCGRG 2 cut(s) 899, 975
BsiSI CCGG 3 cut(s) 470, 999, 1235
BslFI GGGAC 1 cut(s) 517
BslI CCNNNNNNNGG 2 cut(s) 1239, 1294
BsmFI GGGAC 1 cut(s) 517
BsmI GAATGC 1 cut(s) 656
BsnI GGCC 3 cut(s) 814, 1194, 1243
BsoBI CYCGRG 2 cut(s) 899, 975
Bsp1286I GDGCHC 4 cut(s) 937, 993, 1110, 1279
Bsp13I TCCGGA 1 cut(s) 469
Bsp143I GATC 6 cut(s) 330, 595, 712, 725, 943, 1126
BspACI CCGC 1 cut(s) 356
BspANI GGCC 3 cut(s) 814, 1194, 1243
BspCNI CTCAG 4 cut(s) 109, 148, 304, 688
BspEI TCCGGA 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 829, 870
BspPI GGATC 1 cut(s) 603
BspQI GCTCTTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 3 cut(s) 206, 756, 1079
BssECI CCNNGG 6 cut(s) 361, 584, 591, 900, 962, 1233
BssMI GATC 6 cut(s) 330, 595, 712, 725, 943, 1126
BssT1I CCWWGG 4 cut(s) 361, 584, 591, 962
Bst2UI CCWGG 2 cut(s) 574, 1021
Bst4CI ACNGT 3 cut(s) 100, 164, 517
Bst6I CTCTTC 1 cut(s) 436
BstAPI GCANNNNNTGC 1 cut(s) 488
BstC8I GCNNGC 5 cut(s) 354, 537, 554, 740, 959
BstDEI CTNAG 6 cut(s) 117, 135, 312, 375, 696, 719
BstF5I GGATG 6 cut(s) 194, 283, 343, 845, 1229, 1276
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 2 cut(s) 274, 898
BstKTI GATC 6 cut(s) 333, 598, 715, 728, 946, 1129
BstMBI GATC 6 cut(s) 330, 595, 712, 725, 943, 1126
BstMWI GCNNNNNNNGC 3 cut(s) 29, 317, 488
BstNI CCWGG 2 cut(s) 574, 1021
BstNSI RCATGY 3 cut(s) 539, 961, 1335
BstSCI CCNGG 4 cut(s) 572, 997, 1019, 1233
BstSLI GKGCMC 2 cut(s) 937, 993
BstV1I GCAGC 4 cut(s) 49, 70, 304, 478
BstV2I GAAGAC 2 cut(s) 1077, 1122
BstX2I RGATCY 2 cut(s) 595, 725
BstYI RGATCY 2 cut(s) 595, 725
Bsu36I CCTNAGG 1 cut(s) 719
BsuRI GGCC 3 cut(s) 814, 1194, 1243
BtsCI GGATG 6 cut(s) 194, 283, 343, 845, 1229, 1276
BtsIMutI CAGTG 5 cut(s) 160, 513, 654, 763, 1110
Cac8I GCNNGC 5 cut(s) 354, 537, 554, 740, 959
CfoI GCGC 2 cut(s) 274, 898
Cfr13I GGNCC 4 cut(s) 358, 581, 588, 1192
Csp6I GTAC 4 cut(s) 437, 851, 1096, 1250
CviAII CATG 6 cut(s) 334, 536, 930, 958, 1267, 1332
CviQI GTAC 4 cut(s) 437, 851, 1096, 1250
DdeI CTNAG 6 cut(s) 117, 135, 312, 375, 696, 719
DpnI GATC 6 cut(s) 332, 597, 714, 727, 945, 1128
DpnII GATC 6 cut(s) 330, 595, 712, 725, 943, 1126
Eam1104I CTCTTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 436
EciI GGCGGA 1 cut(s) 371
Ecl136II GAGCTC 1 cut(s) 1277
Eco130I CCWWGG 4 cut(s) 361, 584, 591, 962
Eco24I GRGCYC 2 cut(s) 1110, 1279
Eco47I GGWCC 3 cut(s) 358, 581, 588
Eco47III AGCGCT 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 1277
Eco57I CTGAAG 3 cut(s) 107, 200, 1134
Eco81I CCTNAGG 1 cut(s) 719
Eco88I CYCGRG 2 cut(s) 899, 975
EcoICRI GAGCTC 1 cut(s) 1277
EcoRI GAATTC 1 cut(s) 881
EcoRII CCWGG 2 cut(s) 572, 1019
EcoT14I CCWWGG 4 cut(s) 361, 584, 591, 962
EcoT22I ATGCAT 1 cut(s) 1337
EcoT38I GRGCYC 2 cut(s) 1110, 1279
ErhI CCWWGG 4 cut(s) 361, 584, 591, 962
FaeI CATG 6 cut(s) 337, 539, 933, 961, 1270, 1335
FaqI GGGAC 1 cut(s) 517
FatI CATG 6 cut(s) 333, 535, 929, 957, 1266, 1331
FbaI TGATCA 3 cut(s) 330, 712, 943
FblI GTMKAC 1 cut(s) 951
Fnu4HI GCNGC 4 cut(s) 38, 59, 318, 492
FokI GGATG 6 cut(s) 201, 270, 350, 832, 1216, 1283
FriOI GRGCYC 2 cut(s) 1110, 1279
Fsp4HI GCNGC 4 cut(s) 38, 59, 318, 492
FspBI CTAG 3 cut(s) 269, 599, 684
GlaI GCGC 2 cut(s) 273, 897
GluI GCNGC 4 cut(s) 38, 59, 318, 492
GsuI CTGGAG 2 cut(s) 327, 846
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 3 cut(s) 814, 1194, 1243
HapII CCGG 3 cut(s) 470, 999, 1235
HhaI GCGC 2 cut(s) 274, 898
Hin1II CATG 6 cut(s) 337, 539, 933, 961, 1270, 1335
Hin6I GCGC 2 cut(s) 272, 896
HinP1I GCGC 2 cut(s) 272, 896
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 7 cut(s) 211, 466, 473, 566, 692, 745, 821
HpaII CCGG 3 cut(s) 470, 999, 1235
HphI GGTGA 5 cut(s) 707, 722, 1009, 1021, 1061
Hpy166II GTNNAC 5 cut(s) 160, 952, 991, 1098, 1262
Hpy188I TCNGA 6 cut(s) 29, 46, 126, 232, 263, 697
Hpy188III TCNNGA 6 cut(s) 287, 470, 825, 872, 1037, 1216
Hpy8I GTNNAC 5 cut(s) 160, 952, 991, 1098, 1262
HpyCH4III ACNGT 3 cut(s) 100, 164, 517
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 317, 488
HpyF3I CTNAG 6 cut(s) 117, 135, 312, 375, 696, 719
Hsp92II CATG 6 cut(s) 337, 539, 933, 961, 1270, 1335
HspAI GCGC 2 cut(s) 272, 896
Kpn2I TCCGGA 1 cut(s) 469
Ksp22I TGATCA 3 cut(s) 330, 712, 943
Kzo9I GATC 6 cut(s) 330, 595, 712, 725, 943, 1126
LguI GCTCTTC 1 cut(s) 436
LmnI GCTCC 6 cut(s) 77, 308, 735, 827, 1274, 1321
Lsp1109I GCAGC 4 cut(s) 49, 70, 304, 478
LweI GCATC 5 cut(s) 45, 328, 854, 1213, 1344
MaeI CTAG 3 cut(s) 269, 599, 684
MalI GATC 6 cut(s) 332, 597, 714, 727, 945, 1128
MboI GATC 6 cut(s) 330, 595, 712, 725, 943, 1126
MboII GAAGA 9 cut(s) 59, 206, 423, 561, 645, 720, 1082, 1127, 1330
MfeI CAATTG 1 cut(s) 1101
MflI RGATCY 2 cut(s) 595, 725
MhlI GDGCHC 4 cut(s) 937, 993, 1110, 1279
MluCI AATT 8 cut(s) 257, 299, 420, 661, 832, 881, 1101, 1166
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 690
Mph1103I ATGCAT 1 cut(s) 1337
MroI TCCGGA 1 cut(s) 469
MroXI GAANNNNTTC 4 cut(s) 257, 370, 430, 637
MseI TTAA 8 cut(s) 63, 324, 800, 915, 981, 1005, 1065, 1172
MslI CAYNNNNRTG 2 cut(s) 540, 1269
MspA1I CMGCKG 1 cut(s) 40
MspI CCGG 3 cut(s) 470, 999, 1235
MspR9I CCNGG 4 cut(s) 574, 999, 1021, 1235
MunI CAATTG 1 cut(s) 1101
Mva1269I GAATGC 1 cut(s) 656
MvaI CCWGG 2 cut(s) 574, 1021
MwoI GCNNNNNNNGC 3 cut(s) 29, 317, 488
NciI CCSGG 2 cut(s) 999, 1235
NdeII GATC 6 cut(s) 330, 595, 712, 725, 943, 1126
NlaIII CATG 6 cut(s) 337, 539, 933, 961, 1270, 1335
NlaIV GGNNCC 2 cut(s) 829, 870
NsiI ATGCAT 1 cut(s) 1337
NspI RCATGY 3 cut(s) 539, 961, 1335
PaeI GCATGC 2 cut(s) 539, 961
PaeR7I CTCGAG 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 436
PctI GAATGC 1 cut(s) 656
PdmI GAANNNNTTC 4 cut(s) 257, 370, 430, 637
PfeI GAWTC 6 cut(s) 211, 466, 566, 692, 745, 821
PfoI TCCNGGA 1 cut(s) 997
PkrI GCNGC 4 cut(s) 39, 60, 319, 493
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
Psp124BI GAGCTC 1 cut(s) 1279
Psp6I CCWGG 2 cut(s) 572, 1019
PspGI CCWGG 2 cut(s) 572, 1019
PspN4I GGNNCC 2 cut(s) 829, 870
PspPI GGNCC 4 cut(s) 358, 581, 588, 1192
PspXI VCTCGAGB 1 cut(s) 975
PsuI RGATCY 2 cut(s) 595, 725
PvuII CAGCTG 1 cut(s) 40
RsaI GTAC 4 cut(s) 438, 852, 1097, 1251
RsaNI GTAC 4 cut(s) 437, 851, 1096, 1250
RseI CAYNNNNRTG 2 cut(s) 540, 1269
SacI GAGCTC 1 cut(s) 1279
SapI GCTCTTC 1 cut(s) 436
SaqAI TTAA 8 cut(s) 63, 324, 800, 915, 981, 1005, 1065, 1172
SatI GCNGC 4 cut(s) 38, 59, 318, 492
Sau3AI GATC 6 cut(s) 330, 595, 712, 725, 943, 1126
Sau96I GGNCC 4 cut(s) 358, 581, 588, 1192
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 4 cut(s) 574, 999, 1021, 1235
SduI GDGCHC 4 cut(s) 937, 993, 1110, 1279
SfaNI GCATC 5 cut(s) 45, 328, 854, 1213, 1344
Sfr274I CTCGAG 1 cut(s) 975
SinI GGWCC 3 cut(s) 358, 581, 588
SlaI CTCGAG 1 cut(s) 975
SmiMI CAYNNNNRTG 2 cut(s) 540, 1269
SmlI CTYRAG 1 cut(s) 975
SmoI CTYRAG 1 cut(s) 975
SphI GCATGC 2 cut(s) 539, 961
Sse9I AATT 8 cut(s) 257, 299, 420, 661, 832, 881, 1101, 1166
SsiI CCGC 1 cut(s) 356
SspI AATATT 1 cut(s) 910
SspMI CTAG 3 cut(s) 269, 599, 684
SstI GAGCTC 1 cut(s) 1279
StyD4I CCNGG 4 cut(s) 572, 997, 1019, 1233
StyI CCWWGG 4 cut(s) 361, 584, 591, 962
TaaI ACNGT 3 cut(s) 100, 164, 517
TaqI TCGA 2 cut(s) 976, 1341
TasI AATT 8 cut(s) 257, 299, 420, 661, 832, 881, 1101, 1166
TatI WGTACW 2 cut(s) 1095, 1249
TfiI GAWTC 6 cut(s) 211, 466, 566, 692, 745, 821
Tru1I TTAA 8 cut(s) 63, 324, 800, 915, 981, 1005, 1065, 1172
Tru9I TTAA 8 cut(s) 63, 324, 800, 915, 981, 1005, 1065, 1172
TscAI CASTG 5 cut(s) 167, 520, 661, 763, 1117
TseI GCWGC 4 cut(s) 37, 58, 317, 491
TspDTI ATGAA 4 cut(s) 272, 632, 800, 1218
TspGWI ACGGA 1 cut(s) 838
TspRI CASTG 5 cut(s) 167, 520, 661, 763, 1117
VneI GTGCAC 1 cut(s) 989
VpaK11BI GGWCC 3 cut(s) 358, 581, 588
XapI RAATTY 4 cut(s) 299, 661, 881, 1166
XceI RCATGY 3 cut(s) 539, 961, 1335
XhoI CTCGAG 1 cut(s) 975
XmiI GTMKAC 1 cut(s) 951
XmnI GAANNNNTTC 4 cut(s) 257, 370, 430, 637
XspI CTAG 3 cut(s) 269, 599, 684
Zsp2I ATGCAT 1 cut(s) 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.